CN104745650A - Preparation method of combined-state glutamine used for improving nutrients of piglet intestines - Google Patents
Preparation method of combined-state glutamine used for improving nutrients of piglet intestines Download PDFInfo
- Publication number
- CN104745650A CN104745650A CN201510171283.0A CN201510171283A CN104745650A CN 104745650 A CN104745650 A CN 104745650A CN 201510171283 A CN201510171283 A CN 201510171283A CN 104745650 A CN104745650 A CN 104745650A
- Authority
- CN
- China
- Prior art keywords
- preparation
- nutrients
- combined
- glutamine
- improving
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
The invention relates to a preparation method of combined-state glutamine used for improving nutrients of piglet intestines. The preparation method comprises the steps of by taking wheat gluten flour as a raw material, treating the raw material using amylase; then hydrolyzing using alkaline protease/papain, compound protease and trypsase in turn; acidizing zymolyte, heating, deactivating enzyme, centrifuging, filtering superate using micro-filtration membrane, concentrating at reduced pressure and carrying out spray drying to obtain active peptide rich in the combined-state glutamine. Animal experiments show that the combined-state glutamine product can be used for obviously promoting growth and development of camisia foetus of the piglet intestines, and nutrients of the piglet intestines are improved. The preparation method of the combined-state glutamine used for improving the nutrients of the piglet intestines has the characteristics of available raw materials, easy operation, high product activity and large market usage amount.
Description
Technical field
The invention belongs to piglet immunological regulation technology field, being specifically related to a kind of is raw material with gluten powder, and by multi-enzymatic hydrolysis, preparation significantly can improve the method for the combined glutamine of nutrients in intestine of young pigs.
Background technology
Enteron aisle is the main place that animal digestion absorbs nutrient.The intestinal growth of piglet especially weanling pig is still far from perfect, more responsive to the change of external factor, wean bring huge stress and the change of feed shape intestinal mucosa is destroyed, the digestibility of nutrient is caused to reduce, lower immune function, have impact on growing of piglet, and then affect health and the growth of whole growth phase later.Therefore, the intestinal feeding improving piglet is significant.
Glutamine is the indispensable amino acid of human body and animal body.Large quantity research shows, it is not only major storage form amino in body, also there is a series of physiologically active and trophic function simultaneously, main manifestations is: one is the precursor of body cell synthesizing ribonucleotide, it is the main energy sources material of cell (especially lymphocyte and intestinal mucosa cells) of rising in value fast, the immunologic function of body can be improved, promote the growth of intestinal villi film; Two is participation synthesis reduction-state gsh, and improve the resistance of oxidation of body, protection enteron aisle is from toxin infringement and peroxide injury, and minimizing disease and environmental stress condition, to the infringement of body, ensure the eubolism of body; Three is the synthesis promoting protein (especially muscle protein), has the effect of antifatigue.Therefore, important stomach and intestine class medicine is not only by glutamine, is also the conventional supplement that sportsmen regains one's strength fast, is also used widely in Animal nutrition.But free glutamine solvability is low, poor heat stability, is easily converted into L-glutamic acid or Pyrrolidonecarboxylic acid in aqueous, greatly reduces its effect.Therefore, people more expect that preparing binding peptide substitutes free glutamine.
Research shows, combined glutamine not only has the function of free glutamine, and little peptide is more easily absorbed, and utilization ratio is higher.Glutamine peptide mainly ALA-GLN dipeptides and the Glycyl-glutaminedipeptide applied at present, but owing to being synthetic, it is expensive, applies Financial cost higher in production reality.Therefore, to be rich in the protein of glutamine for raw material, preparing binding peptide glutamine by enzyme solution is the choice of technology economically and reasonably.
Gluten powder is the product after being removed by the starch in whole meal flour, and protein content is up to 80%.Glutamine content in gluten powder, up to more than 33%, is vegetable-proteins maximum containing glutamine at present.Can obtain the biological peptide containing combined glutamine by enzymolysis, compared with the glutamine dipeptide of synthetic, its price advantage clearly.
The Research Literature of current hydrolysis gluten powder (wheat protein) is more, obtain the wheat protein zymolyte with difference in functionality character or feature, as Pan Lijun " two enzyme fractional hydrolysis legal system is for the research of bioactive glutamine oligopeptide " (foodstuffs industry science and technology, 2008), paddy China " research of the little peptide of wheat-gluten enzyme-squash techniqued suitable molecular weight " (foodstuffs industry science and technology, 2013) used 4 kinds of proteolytic enzyme, directly gluten powder enzymolysis has been investigated to hydrolysis effect of different enzyme respectively with single proteolytic enzyme.Yang get Sheng adopts the combination of 2 kinds of proteolytic enzyme to study in " hydrolysis mucedin efficiently prepares the research of glutamine-bound peptides " (Food science, 2006).Academic dissertation about wheat protein enzymolysis also has some sections, as Lv Yan " enzymolysis wheat protein produces the research of the active skin of paddy ammonia phthalein amine " (Zhejiang University, 2008) acid protease hydrolysis; Zhang Haihua " enzyme-squash techniqued of wheat-gluten source glutamine peptide, structural analysis and Study on Physiological Activity " (Southern Yangtze University, 2011) middle hydrolysis by novo; Individually employ multiple protein enzyme in Kong Xiangzhen " enzymolysis of wheat mucedin and the research of opioid peptides thereof " (Southern Yangtze University, 2007) to study; Hong Jinfang " enzymolysis wheat protein is on the impact of heat-stressed mice physiological function " (University Of Agriculture and Forestry In Fujian, 2009) use composite protease hydrolysis; Zhang Li " preparation of paddy ammonia phthalein amine bioactive peptide and character research " (HeFei University of Technology, 2009) adopts Sumizyme MP and trypsinase; Li Qingli " the enzymolysis reparation technology research of gluten powder bioactive peptide " (He'nan University of Technology, 2007) adopts the single enzymolysis in trypsinase, flavor protease and compound protease and two enzyme to combine hydrolysis etc.
There is the enzymolysis also relating to wheat protein in many sections of patent documentations." a kind of industrialized preparing process CN200910065614.7 of hydrolyzed wheat protein for feeding " as Xin Wei company adopts Sumizyme MP and prozyme two step hydrolysis method; Chun Fa company " a kind of preparation method CN201110216644.0 of wheat gluten hydrolyzate " adopts phosphoric acid pre-treatment reacting by heating to add alkali lye+papain, trypsin mixture again after 2 hours, is finally hydrolyzed with food flavor enzyme again; It the nine company industrial process CN200810015396.1 of Enzymatic wheat peptide " from the gluten powder " adopts combinative enzyme hydrolysis to react 8 hours; Southern Yangtze University etc. " a kind of method CN200910231736.9 of preparing wheat protein source opioid peptide by enzymolysis of wheat protein " adopt stomach en-to react 9 ~ 24 hours, add trypsin hydrolyzing again 4 ~ 6 hours, go out enzyme centrifuged supernatant decolorizing with activated carbon, then ultrafiltration, dry product.One-tenth medicine company far away " a kind of extracting method CN201010540566.5 of wheat gluten peptide " adds ethanol and S-WAT and after boiling a few hours in material, successively with alkaline enzyme and food flavor enzyme hydrolysis, then decolour, desalination, concentrated, drying obtain wheat gluten peptide; Lian Hai company " a kind of production method CN201310191254.1 of hydrolyzed wheat protein " adopts material first to use amylase and Glucoamylase hydrolysis again with after combinative enzyme hydrolysis, last inoculation yeast bacterium fermentation.
Although above-mentioned research is the enzymolysis of gluten powder or wheat protein, because the hydrolysis properties of various proteolytic enzyme is different with enzymolysis order, the character of its enzymolysis product and function is caused also to have very big-difference.But rarely have to prepare that can to improve nutrients in intestine of young pigs be main goal in research, be detected in Liu great writer's " preparation of glutenin source glutamine peptide and intestinal feeding Effect disquisition " (Hua Zhong Agriculture University, 2008), but the document is only after being removed by the prolamine in wheat gluten flour, be raw material with glutenin wherein, use trypsinase and hydrolysis by novo again, adopt gluten powder to be raw material with the present invention, have essence different by amylase with the sequential hydrolysis method of proteolytic enzyme.
Summary of the invention
In view of glutamine to animal growth, there is material impact, free glutamine is unstable, synthesis glutamine dipeptide cost is high, be not suitable for the features such as extensive application, the invention provides a kind of preparation method of combined glutamine, to be rich in the wheat gluten flour of glutamine for raw material, adopt the method for amylase and proteolytic enzyme sequential hydrolysis, prepare and the combined glutamine having unusual effect is grown to Small Intestine of Piglets chorion.
For solving the problem, the present invention is achieved through the following technical solutions:
Be first the dextrin of suitable size by the Starch Hydrolysis in gluten powder with amylase, make it be conducive to participating in the generation of functional product in following protein enzymic hydrolysis process.On the basis of aforementioned amylase process, in a certain order, successively use the different loci in different proteolytic enzyme directionally hydrolyzing gluten powder albumen, be degraded to the little peptide molecule with specific function.Specifically comprise the following steps:
(1) get gluten powder to mix by the weight ratio of 1:2 ~ 5 with 65 ~ 75 DEG C of water, after 15 ~ 50 minutes, amylase is added again by 0.1 ~ 0.3wt% of current material gross weight, react after 0.5 ~ 2.0 hour, this material is cooled to 50 ~ 55 DEG C, with alkali lye adjustment pH to 8.0 ~ 10.0, then adds the mixed enzyme of Sumizyme MP and papoid by 0.2 ~ 1.2wt% of material, the two weight ratio is 1:1, then insulation reaction 1.0 ~ 2.5 hours; Add compound protease according to 0.2 ~ 1.0wt% of upper step gained material gross weight again, react 1.0 ~ 2.5 hours under agitation condition; Upper step gained temperature of charge is adjusted to 35 ~ 38 DEG C, with alkali lye adjustment pH to 7.5 ~ 8.0, adds trypsinase according to 0.05 ~ 0.3wt% of current material gross weight, stirring reaction 2.0 ~ 3.5 hours;
(2) regulate upper step gained material pH to 4.3 ~ 4.8 with acid, be heated to 100 DEG C of enzymes 15 ~ 20 minutes of going out, the centrifugal disgorging of 2500 ~ 3500 turns/min;
(3) by the ultrafiltration of upper step gained centrifuged supernatant microfiltration membrane, then through concentrated, pressure spray dryer, the meal containing combined glutamine is obtained.
Described amylase is preferably at least one in mesophilicα-diastase, fungal alpha-amylase.
Alkali lye used is preferably the sodium hydroxide solution of 0.5 ~ 2.0 mol/L.
Acid used is preferably the hydrochloric acid of 0.5 ~ 2.0mol/L.
The preferred aperture of described microfiltration membrane is 0.45 micron.
positive beneficial effect of the present invention:
The present invention is on the basis of large quantity research, determine first with after amylase process, again according to specific order, the proteolytic enzyme deriving from animals and plants and microorganism with four kinds is hydrolyzed gluten powder successively, through the preparation such as acid precipitation, membrane separation technique process combined glutamine.Prove through animal experiment, this combined glutamine has and improves nutrients in intestine of young pigs, promotes that Small Intestine of Piglets chorion grows, improves the effects such as piglet anti-stress ability.
(1) first the present invention uses amylase that the starch polymer in gluten powder is degraded to micromolecular dextrin or reducing sugar, reduces the interaction of starch and protein, then, uses the protein in different protease hydrolysis materials successively according to particular order.
(2) reaction feed liquid is adjusted to specific pH scope and is reheated by the present invention, and this is not only conducive to the enzyme that goes out fast, the macro-molecular protein of mild hydrolysis also can be made to precipitate, be more conducive to the separation of small-molecular peptides.
(3) more than 90% be the composition that molecular weight is less than 2000 Da in products therefrom of the present invention by analysis, the content of combined glutamine is greater than 29.1%, and the content of free glutamine is less than 0.3%.Through animal feeding trials, this product to improving nutrients in intestine of young pigs, promote that its intestinal villi film has grown unusual effect.
(4) the raw materials used source of the present invention is wide, and has the advantages such as easy to operate, its lytic activity is high, wide market.
Accompanying drawing explanation
Fig. 1 is the molecular weight collection of illustrative plates (gel chromatographic columns analysis) of the invention process method 1 products therefrom;
Fig. 2 is the molecular weight collection of illustrative plates (gel chromatographic columns analysis) of the invention process method 2 products therefrom;
Fig. 3 is the molecular weight collection of illustrative plates (gel chromatographic columns analysis) of the invention process method 3 products therefrom.
Embodiment
Below in conjunction with specific embodiment, the present invention is described further, but the present invention is not limited to following examples.
embodiment 1for improving the preparation method of the combined glutamine of nutrients in intestine of young pigs, comprise the steps:
Gluten powder is mixed by 1:3 weight ratio with 70 DEG C of hot water, be incubated 45 minutes in reactor after, then adds mesophilicα-diastase by the 0.15wt% of current material (gluten powder+hot water) weight, react 1.5 hours; Material is cooled to 50 DEG C, with 1mol/L sodium hydroxide solution adjustment pH to 9.0, add Sumizyme MP and papoid (the two weight ratio is 1:1) by the 1.0wt% of current material (gluten powder+hot water+mesophilicα-diastase+sodium hydroxide solution) gross weight again, react 2.0 hours; Add compound protease by the 0.8wt% of current material (gluten powder+hot water+mesophilicα-diastase+pH adjusting agent+Sumizyme MP+papoid) gross weight again, react 1.5 hours under agitation condition; Temperature of charge is adjusted to 37 DEG C, adjustment pH to 7.8, adds trypsinase, stirring reaction 2.5 hours by the 0.1wt% of current material (gluten powder+hot water+α-middle temperature amylase+pH adjusting agent+Sumizyme MP+papoid+compound protease) gross weight; With 1mol/L hydrochloric acid joint material pH to 4.5 ~ 4.6, be heated to 100 DEG C of enzymes 18 minutes of going out, then with the centrifugal disgorging of 2800 turns/min; Supernatant liquor CMW 2000 microfiltration membrane ultrafiltration, then through concentrated, pressure spray dryer, obtains meal.
Analyze with TSKgel G2000 SWXL gel chromatographic columns, the molecular weight collection of illustrative plates of this product as shown in Figure 1.(cytochrome C is respectively, MW12500 by known molecular amount standard peptide; Trypsin inhibitor,Trasylol, MW6500; Bacillus enzyme, MW1450; Second aminoacyl-second aminoacyl-tyrosyl-arginine, MW451; Second aminoacyl-second aminoacyl-glycocoll, MW189) gel chromatography analysis and calculation under the same conditions, the component concentration that this product middle-molecular-weihydroxyethyl is less than 2000Da is 93.5%.Known through amino acid analysis, wherein the content of combined glutamine is 29.1%, and the content of free state glutamine is 0.25% (analytical procedure is with reference to Chinese patent literature CN201410131180.7).
Piglet application test: the Du great Chang three way cross weanling pig application test selecting 14 age in days wean, adds the present embodiment product 2.5wt%, 20 days test periods, with the person that adds wheat protein for contrast in daily ration.Result shows, and the average daily gain of test group piglet improves 8.05% than control group, and the price of deed improves 9.3%; Feed 5 days time, the width of test group piglet duodenum, jejunum and ileum than contrast component you can well imagine high by 12.2%, 5.3% and 11.2%, improve 12.5%, 6.6% and 14.1% highly respectively.Show that gained combined glutamine of the present invention has good intestinal feeding effect than wheat protein.
embodiment 2for improving the preparation method of the combined glutamine of nutrients in intestine of young pigs, comprise the steps:
After gluten powder being mixed 15 minutes with 65 DEG C of hot water by 1:2 weight ratio in reactor, then add fungal alpha-amylase by 0.1% of current weight of material, react 0.5 hour; After material being dropped to 55 DEG C, be 8.0 with 0.5mol/L sodium hydroxide solution adjustment pH, then add Sumizyme MP and papoid (the two weight ratio is 1:1) by 1.2% of current weight of material, react 1.0 hours; Add compound protease by 1.0% of current weight of material again, react 1.0 hours; Temperature of charge is adjusted to 35 DEG C, adjustment pH is 7.5, adds trypsinase, stirring reaction 2.0 hours by 0.05% of current weight of material; With 0.5mol/L salt acid for adjusting pH to 4.3 ~ 4.4, be heated to 100 DEG C of enzymes 15 minutes of going out, then with the centrifugal disgorging of 2500 turns/min; Supernatant liquor CMW 2000 microfiltration membrane ultrafiltration, then through concentrated, pressure spray dryer, obtains meal.
Analyze with TSKgel G2000 SWXL gel chromatographic columns, the molecular weight collection of illustrative plates of this product as shown in Figure 2.By the analysis and calculation of known molecular amount standard peptide, in this product, the component molecular amount of 92.1% is less than 2000Da.Known through amino acid analysis, wherein the content of combined glutamine is 28.9%, and the content of free state glutamine is 0.25%.
Select 14 age in days Du to greatly enhance the display of three way cross weanling pig application test, add the present embodiment product 2.5wt% in daily ration, with the person that adds wheat protein for contrast.Result shows, and in 20 days test periods, the average daily gain of test group piglet improves 8.11% than control group, and the price of deed improves 9.5%; Feed 5 days time, the width of test group piglet duodenum, jejunum and ileum than contrast component you can well imagine high by 12.1%, 5.4% and 11.3%, improve 12.7%, 6.5% and 14.3% highly respectively.Show that combined glutamine of the present invention has good intestinal feeding effect than wheat protein, and there is good stability.
embodiment 3for improving the preparation method of the combined glutamine of nutrients in intestine of young pigs, comprise the steps:
After gluten powder being mixed 50 minutes with 75 DEG C of hot water by weight 1:5 in reactor, then add mesophilicα-diastase by 0.3% of current weight of material, react 2 hours; After material being cooled to 55 DEG C, with 2mol/L sodium hydroxide solution adjustment pH to 10.0, then add Sumizyme MP and papoid (the two weight ratio is 1:1) by 0.2% of current weight of material, react 2.5 hours; Add compound protease according to 1.0% of current weight of material, react 2.5 hours; Temperature of charge is adjusted to 38 DEG C, pH is adjusted to 8.0, adds trypsinase by 0.3% of current weight of material, reacts 3.5 hours; With 2mol/L salt acid for adjusting pH to 4.8, be heated to 100 DEG C of enzymes 20 minutes of going out, then with the centrifugal disgorging of 3000 turns/min; Supernatant liquor CMW 2000 microfiltration membrane ultrafiltration, then through concentrated, pressure spray dryer, obtains meal.
Collection of illustrative plates is analyzed as shown in Figure 3 with TSKgel G2000 SWXL gel chromatographic columns.In this product, the component molecular amount of 91.6% is less than 2000Da.The content of combined glutamine is 29.4%, and the content of free state glutamine is 0.20%.
Select 14 age in days Du to greatly enhance the display of three way cross weanling pig application test, add the present embodiment product 2.5wt% in daily ration, with the person that adds soybean protein isolate for contrast.Result shows, and in 20 days test periods, the average daily gain of test group piglet improves 8.77% than control group, and the price of deed improves 10.7%; Feed 5 days time, the width of test group piglet duodenum, jejunum and ileum than contrast component you can well imagine high by 13.2%, 6.8% and 13.1%, improve 13.9%, 7.9% and 16.1% highly respectively.Show that combined glutamine of the present invention has good intestinal feeding effect than soybean separation.
Claims (5)
1., for improving a preparation method for the combined glutamine of nutrients in intestine of young pigs, it is characterized in that, comprise the following steps:
(1) get gluten powder to mix by the weight ratio of 1:2 ~ 5 with 65 ~ 75 DEG C of water, after 15 ~ 50 minutes, amylase is added again by 0.1 ~ 0.3wt% of current material gross weight, react after 0.5 ~ 2.0 hour, this material is cooled to 50 ~ 55 DEG C, with alkali lye adjustment pH to 8.0 ~ 10.0, the mixed enzyme of Sumizyme MP and papoid is added again by 0.2 ~ 1.2wt% of material, the two weight ratio is 1:1, then insulation reaction 1.0 ~ 2.5 hours, compound protease is added again according to 0.2 ~ 1.0wt% of gained material gross weight, react under agitation condition after 1.0 ~ 2.5 hours, gained temperature of charge is adjusted to 35 ~ 38 DEG C, with alkali lye adjustment pH to 7.5 ~ 8.0, trypsinase is added according to 0.05 ~ 0.3wt% of current material gross weight, stirring reaction 2.0 ~ 3.5 hours,
(2) regulate upper step gained material pH to 4.3 ~ 4.8 with acid, be heated to 100 DEG C of enzymes 15 ~ 20 minutes of going out, the centrifugal disgorging of 2500 ~ 3500 turns/min;
(3) by the ultrafiltration of upper step gained centrifuged supernatant microfiltration membrane, then through concentrated, pressure spray dryer, the meal containing combined glutamine is obtained.
2., according to claim 1 for improving the preparation method of the combined glutamine of nutrients in intestine of young pigs, it is characterized in that: described amylase is at least one in mesophilicα-diastase, fungal alpha-amylase.
3., according to claim 1 for improving the preparation method of the combined glutamine of nutrients in intestine of young pigs, it is characterized in that: in described step (1), described alkali lye is the sodium hydroxide solution of 0.5 ~ 2.0mol/L.
4., according to claim 1 for improving the preparation method of the combined glutamine of nutrients in intestine of young pigs, it is characterized in that: in described step (2), acid used is the hydrochloric acid of 0.5 ~ 2.0mol/L.
5. according to claim 1 for improving the preparation method of the combined glutamine of nutrients in intestine of young pigs, it is characterized in that: in described step (3), the aperture of microfiltration membrane used is 0.45 micron.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510171283.0A CN104745650B (en) | 2015-04-13 | 2015-04-13 | For the preparation method for the reference state glutamine for improving nutrients in intestine of young pigs |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510171283.0A CN104745650B (en) | 2015-04-13 | 2015-04-13 | For the preparation method for the reference state glutamine for improving nutrients in intestine of young pigs |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104745650A true CN104745650A (en) | 2015-07-01 |
| CN104745650B CN104745650B (en) | 2018-02-02 |
Family
ID=53585905
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510171283.0A Active CN104745650B (en) | 2015-04-13 | 2015-04-13 | For the preparation method for the reference state glutamine for improving nutrients in intestine of young pigs |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104745650B (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113317398A (en) * | 2021-02-08 | 2021-08-31 | 福建傲农生物科技集团股份有限公司 | Piglet premix and preparation method and application thereof |
| CN114990185A (en) * | 2022-07-07 | 2022-09-02 | 卫仕宠物营养科学研究院(江苏)有限公司 | Preparation method of oligopeptide capable of improving defecation function of pet dogs and cats |
| CN115521963A (en) * | 2022-10-28 | 2022-12-27 | 华南理工大学 | Glutamine peptide and preparation method and application thereof |
| CN115736092A (en) * | 2022-12-14 | 2023-03-07 | 山东泰山生力源集团股份有限公司 | Method for improving glutamine content in feed through solid state fermentation |
| CN117099869A (en) * | 2023-06-27 | 2023-11-24 | 河南工业大学 | Preparation method of wheat protein peptide chelated calcium |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101627793A (en) * | 2009-07-31 | 2010-01-20 | 郑州新威营养技术有限公司 | Industrial production method of hydrolyzed wheat protein for feeding |
| CN102326664A (en) * | 2011-07-29 | 2012-01-25 | 天津春发生物科技集团有限公司 | Preparation method of wheat gluten hydrolyzate |
| CN103642885A (en) * | 2013-11-26 | 2014-03-19 | 郑州市中食农产品加工研究院 | Method for producing wheat biological active peptide by fermentation |
| CN104263794A (en) * | 2014-09-30 | 2015-01-07 | 青岛嘉瑞生物技术有限公司 | Process technique for preparing wheat gluten powder peptide by fermentation and enzymolysis method |
-
2015
- 2015-04-13 CN CN201510171283.0A patent/CN104745650B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101627793A (en) * | 2009-07-31 | 2010-01-20 | 郑州新威营养技术有限公司 | Industrial production method of hydrolyzed wheat protein for feeding |
| CN102326664A (en) * | 2011-07-29 | 2012-01-25 | 天津春发生物科技集团有限公司 | Preparation method of wheat gluten hydrolyzate |
| CN103642885A (en) * | 2013-11-26 | 2014-03-19 | 郑州市中食农产品加工研究院 | Method for producing wheat biological active peptide by fermentation |
| CN104263794A (en) * | 2014-09-30 | 2015-01-07 | 青岛嘉瑞生物技术有限公司 | Process technique for preparing wheat gluten powder peptide by fermentation and enzymolysis method |
Non-Patent Citations (3)
| Title |
|---|
| 杨得胜: "谷氨酰胺结合肽的高效制备", 《南京农业大学学位论文》 * |
| 潘丽军等: "双酶分步水解法制备谷氨酰胺活性肽的研究", 《食品工业科技》 * |
| 郑根华: "谷氨酞胺对断奶仔猪的影响及其机理研究", 《浙江大学学位论文》 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113317398A (en) * | 2021-02-08 | 2021-08-31 | 福建傲农生物科技集团股份有限公司 | Piglet premix and preparation method and application thereof |
| CN114990185A (en) * | 2022-07-07 | 2022-09-02 | 卫仕宠物营养科学研究院(江苏)有限公司 | Preparation method of oligopeptide capable of improving defecation function of pet dogs and cats |
| CN114990185B (en) * | 2022-07-07 | 2023-08-08 | 卫仕宠物营养科学研究院(江苏)有限公司 | Preparation method of oligopeptide for improving defecation function of pet dogs and cats |
| CN115521963A (en) * | 2022-10-28 | 2022-12-27 | 华南理工大学 | Glutamine peptide and preparation method and application thereof |
| CN115736092A (en) * | 2022-12-14 | 2023-03-07 | 山东泰山生力源集团股份有限公司 | Method for improving glutamine content in feed through solid state fermentation |
| CN117099869A (en) * | 2023-06-27 | 2023-11-24 | 河南工业大学 | Preparation method of wheat protein peptide chelated calcium |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104745650B (en) | 2018-02-02 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101096696B (en) | Industrial production method of corn protein polypeptide from corn protein powder by enzymatical process | |
| US9609883B2 (en) | Method for producing wheat glutamine peptide | |
| CN102048022B (en) | Soybean polypeptide without hydrolysis and bitter tastes as well as preparation method and application thereof | |
| CN101766253B (en) | Method for preparing rice protein polypeptide powder from rice residue protein | |
| CN104059958B (en) | A kind of production and processing technology of dried porcine saluble | |
| CN104745650A (en) | Preparation method of combined-state glutamine used for improving nutrients of piglet intestines | |
| CN103271221B (en) | Method for producing hydrolyzed wheat protein | |
| CN102115734B (en) | Special compound enzyme for yeast hydrolysis and preparation method thereof | |
| CN101126103A (en) | Method for preparing corn protein peptide with high F valve by microorganism zymotechnics | |
| CN104186918A (en) | Preparing method of corn gluten polypeptide for fodder | |
| CN102660622A (en) | Method for preparing bioactive soybean peptide by mixed culture solid-state fermentation | |
| CN102907559A (en) | Process for industrial production of hydrolyzed wheat protein | |
| CN104212870A (en) | Process for fermentation production of lysine hydrochloride | |
| CN103461648B (en) | Method for preparing feed by virtue of secondary fermentation of amino acid fermentation hyphae | |
| CN106916871A (en) | A kind of bionic enzymatic prepares the production method of freshwater mussel meat small peptide | |
| CN108893515B (en) | High F value oligopeptide and preparation method thereof | |
| CN111748598A (en) | Small molecule peptide protein powder and preparation method thereof | |
| CN105494886A (en) | Hydrolyzed protein powder and preparation method thereof | |
| CN102228125B (en) | Preparation method of algal active peptide | |
| CN109601736A (en) | A kind of bacterium enzyme collaboration solid state fermentation pannage and preparation method thereof | |
| CN101613734B (en) | Method for preparing plasma polypeptide | |
| CN107080263A (en) | A kind of bionic enzymatic prepares the production method of pea Gly-His-Lys | |
| CN102669409B (en) | Method for preparing fermentation promoting peptide of fermented feed from mushroom residue | |
| CN103642885A (en) | Method for producing wheat biological active peptide by fermentation | |
| CN109486884A (en) | A kind of preparation method of soybean minor peptides |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |