CN105852104A - Preparation method of health-care instantly-dissolved protein powder - Google Patents

Preparation method of health-care instantly-dissolved protein powder Download PDF

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Publication number
CN105852104A
CN105852104A CN201610241962.5A CN201610241962A CN105852104A CN 105852104 A CN105852104 A CN 105852104A CN 201610241962 A CN201610241962 A CN 201610241962A CN 105852104 A CN105852104 A CN 105852104A
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CN
China
Prior art keywords
protein powder
bacillus subtilis
food
minutes
seed liquor
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201610241962.5A
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Chinese (zh)
Inventor
吕庆茂
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Harbin Weiping Technology Development Co Ltd
Original Assignee
Harbin Weiping Technology Development Co Ltd
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Priority to CN201610241962.5A priority Critical patent/CN105852104A/en
Publication of CN105852104A publication Critical patent/CN105852104A/en
Pending legal-status Critical Current

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Classifications

    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23JPROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
    • A23J3/00Working-up of proteins for foodstuffs
    • A23J3/30Working-up of proteins for foodstuffs by hydrolysis
    • A23J3/32Working-up of proteins for foodstuffs by hydrolysis using chemical agents
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23JPROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
    • A23J3/00Working-up of proteins for foodstuffs
    • A23J3/14Vegetable proteins
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20Bacteria; Culture media therefor
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs

Abstract

The invention belongs to the technical field of food processing, and particularly relates to a preparation method of health-care instantly-dissolved protein powder. The method comprises the steps that food-grade rice protein powder and food-grade corn protein powder are weighed and mixed, a glucose solution is added into the mixture and stirred to be uniform, sterilization is conducted, bacillus subtilis is inoculated for fermentation, boiling and cooling are conducted after fermentation is completed, and sterilized fermentation liquor is obtained; mung bean sprouts and the sterilized fermentation liquor are mixed and sent into a colloid mill, a mixed slurry is obtained, the mixed slurry is hydrolyzed and filtered, filtrate is obtained and subjected to concentrating and spray-drying, and the finished product is obtained. The health-care instantly-dissolved protein powder prepared through the method is rich in amino acid and small peptide, contains a large number of functional ingredients of gamma-aminobutyric acid and the like, is easy to absorb and has multiple effects of improving the organism immunity, lowering blood pressure, strengthening the brain and the like.

Description

A kind of preparation method of health instant protein powder
Technical field
The invention belongs to food processing technology field, be specifically related to the preparation method of a kind of health instant protein powder.
Background technology
Protein is mainly derived from animal food and plant food, the essential amino acid A wide selection of colours and designs of animal protein, and ratio is reasonable, is therefore easier to digestion than general vegetable protein, absorbs and utilize, and nutritive value is the most relatively high.And vegetable protein is owing to lacking certain essential amino acid, the degree causing vegetable protein to be absorbed and utilized by the body also can differ from.The approach improving plant protein nutrition effect has two, and first,
By different types of vegetable protein mixed edible, such as mix edible by corn gluten protein and peas protein, then the two contained essential amino acid can be learnt from other's strong points to offset one's weaknesses, and is complementary to one another, can reach or close to the level of animal protein, the utilization rate of protein can be improved;Second, vegetable protein is degraded into small molecular protein polypeptide, after cheap vegetable protein is degraded into small molecular protein polypeptide, although the amino acid content of polypeptide is consistent with kind protein corresponding thereto, but polypeptide has its corresponding unexistent biologically actives of protein many, such as rice protein polypeptide can be hypotensive, promote fat metabolism, reduce serum cholesterol, rice protein does not then have this to act on, zein polypeptide has raising immunity of organisms, protect the liver, sober up, hypotensive grade acts on, and zein does not has this to act on.
Most plants polypeptide has the effect of suppression Angiotensin-Converting, thus reduces blood pressure, and Gabanergic acts on the vasomotor center of spinal cord, effectively facilitates blood vessel dilatation, reduces the purpose of blood pressure.GABA and vegetable protein polypeptide act synergistically, and can reach obvious antihypertensive effect, additionally, GABA also has brain tonic and intelligence development, anti-epileptic, promote sleep, moisturizing of improving looks, and delay cerebral senility to wait for a long time function.GABA is distributed widely in the seed of beans, cereal etc., and the beans after particularly germinateing and cereal, its alpha-aminobutyric acid content can increase several times to decades of times.
Summary of the invention
It is an object of the invention to provide the preparation method of a kind of health instant protein powder rich in the functional component such as polypeptide and GABA.
The object of the present invention is achieved like this:
Food-grade rice protein powder and food-grade corn albumen powder is weighed respectively according to the ratio of 1:1, mix, add the glucose solution of 1% concentration of egg mix 15-20 times of quality of white powder, stir, pH is adjusted to 6.8-7.2, sterilizing 20 minutes under the conditions of 121 DEG C, cool to 36-38 DEG C, inoculate bacillus subtilis seed liquor by the inoculum concentration of 5-10%, shaking table top fermentation 30-36 hour of 180-200rpm, 36-38 DEG C, be cooled to after then boiling 10 minutes 35-40 DEG C sterilization fermentation liquid.
Weigh the mung bean sprouts of certain mass, add the sterilization fermentation liquid of 10-15 times of quality, cross colloid mill and obtain mixed slurry, mixed slurry pH is adjusted to 4.8-5.2, maintain 4-5 hour under the conditions of 37 DEG C, then be warmed up to 50-55 DEG C, keep 2-4 hour, then filter to get filtrate after boiling 10 minutes, filtrate is the most concentrated, be spray-dried after get product.
Present invention additionally comprises following feature:
The preparation method of described bacillus subtilis seed liquor is: weighs peptone 5g, beef extract 3g, glucose 4g, sodium chloride 5g, adds water and be settled to 1000ml, pH is adjusted to 6.8-7.2, obtains bacillus subtilis seed liquor culture medium.Take 100ml seed liquor to cultivate based in 500ml triangular flask, 121 DEG C of sterilizings 20 minutes, be cooled to 36-38 DEG C, inoculate bacillus subtilis, the shaking table of 180-200rpm, 36-38 DEG C is cultivated 18-22 hour.
The health instant protein powder made by the inventive method, containing abundant amino acid and little peptide, simultaneously possibly together with functional components such as substantial amounts of GABAs, the most easily absorbs, and has the raising multiple efficacies such as immunity of organisms, hypotensive, brain tonic.
Specific embodiments
The Strains B. subtilis CICC21095 that the present invention uses purchases in Chinese industrial Microbiological Culture Collection administrative center.
Weigh peptone 5g, beef extract 3g, glucose 4g, sodium chloride 5g, add water and be settled to 1000ml, pH is adjusted to 7.0, obtain bacillus subtilis seed liquor culture medium.Take 100ml seed liquor to cultivate based in 500ml triangular flask, 121 DEG C of sterilizings 20 minutes, be cooled to 37 DEG C, inoculate bacillus subtilis, 190rpm, the shaking table of 37 DEG C are cultivated and within 20 hours, i.e. obtains bacillus subtilis seed liquor.
Food-grade rice protein powder and food-grade corn albumen powder is weighed respectively according to the ratio of 1:1, mix, add the glucose solution of 1% concentration of 18 times of quality of egg mix white powder, stir, pH is adjusted to 7.0, sterilizing 20 minutes under the conditions of 121 DEG C, cool to 37 DEG C, inoculum concentration by 8% inoculates bacillus subtilis seed liquor, in 190rpm, the shaking table top fermentation 33 hours of 37 DEG C, be cooled to after then boiling 10 minutes 37 DEG C sterilization fermentation liquid.
Weigh the mung bean sprouts of certain mass, add the sterilization fermentation liquid of 10 times of quality, cross colloid mill and obtain mixed slurry, mixed slurry pH is adjusted to 5.0, maintain 4.5 hours under the conditions of 37 DEG C, then be warmed up to 50 DEG C, keep 3 hours, then filter to get filtrate after boiling 10 minutes, filtrate is the most concentrated, be spray-dried after get product.

Claims (1)

1. a preparation method for health instant protein powder, is characterized in that being prepared from through following process:
(1) weigh peptone 5g, beef extract 3g, glucose 4g, sodium chloride 5g, add water and be settled to 1000ml, pH is adjusted to 6.8-7.2, obtains bacillus subtilis seed liquor culture medium, take 100ml seed liquor and cultivate based in 500ml triangular flask, 121 DEG C of sterilizings 20 minutes, it is cooled to 36-38 DEG C, inoculates bacillus subtilis, the shaking table of 180-200rpm, 36-38 DEG C is cultivated and within 18-22 hour, i.e. obtains bacillus subtilis seed liquor;
(2) weigh food-grade rice protein powder and food-grade corn albumen powder respectively according to the ratio of 1:1, mix, add the glucose solution of 1% concentration of egg mix 15-20 times of quality of white powder, stir, pH is adjusted to 6.8-7.2, sterilizing 20 minutes under the conditions of 121 DEG C, cool to 36-38 DEG C, bacillus subtilis seed liquor is inoculated by the inoculum concentration of 5-10%, shaking table top fermentation 30-36 hour of 180-200rpm, 36-38 DEG C, be cooled to after then boiling 10 minutes 35-40 DEG C sterilization fermentation liquid;
(3) weigh the mung bean sprouts of certain mass, add the sterilization fermentation liquid of 10-15 times of quality, cross colloid mill and obtain mixed slurry, mixed slurry pH is adjusted to 4.8-5.2, maintain 4-5 hour under the conditions of 37 DEG C, then be warmed up to 50-55 DEG C, keep 2-4 hour, then filter to get filtrate after boiling 10 minutes, filtrate is the most concentrated, be spray-dried after get product.
CN201610241962.5A 2016-04-19 2016-04-19 Preparation method of health-care instantly-dissolved protein powder Pending CN105852104A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201610241962.5A CN105852104A (en) 2016-04-19 2016-04-19 Preparation method of health-care instantly-dissolved protein powder

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201610241962.5A CN105852104A (en) 2016-04-19 2016-04-19 Preparation method of health-care instantly-dissolved protein powder

Publications (1)

Publication Number Publication Date
CN105852104A true CN105852104A (en) 2016-08-17

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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106722325A (en) * 2017-01-14 2017-05-31 哈尔滨伟平科技开发有限公司 The preparation method of chicken meal
CN106819956A (en) * 2017-01-14 2017-06-13 哈尔滨伟平科技开发有限公司 A kind of preparation method of shrimp med
CN106889466A (en) * 2017-01-15 2017-06-27 哈尔滨伟平科技开发有限公司 A kind of preparation method of compound nutritional soy meal
CN107772142A (en) * 2016-08-26 2018-03-09 海盐县金利达饲料有限公司 A kind of feed grade fermentation rice meal rich in neutral proteinase and preparation method thereof
CN108283259A (en) * 2018-01-02 2018-07-17 中国农业科学院农产品加工研究所 Health-care instant corn meal and preparation method thereof

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CN102719505A (en) * 2012-05-14 2012-10-10 梁金钟 Method for preparing vegetable protein small peptide by microoprganism fermentation
CN103919231A (en) * 2014-05-06 2014-07-16 恩施州益寿果业股份有限公司 Preparation method of vitamin C-rich composite kiwi fruit powder
CN104082522A (en) * 2014-07-29 2014-10-08 哈尔滨伟平科技开发有限公司 Preparation method for healthcare instant soybean powder
CN104087637A (en) * 2014-07-29 2014-10-08 哈尔滨伟平科技开发有限公司 Method for preparing vegetable protein polypeptide by virtue of microbiological fermentation
CN104394319A (en) * 2014-11-24 2015-03-04 广州市浩云安防科技股份有限公司 Embedded HD network video recorder
CN105104978A (en) * 2015-09-08 2015-12-02 福建农林大学 Sprouting cereals flour and preparation method thereof

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102719505A (en) * 2012-05-14 2012-10-10 梁金钟 Method for preparing vegetable protein small peptide by microoprganism fermentation
CN103919231A (en) * 2014-05-06 2014-07-16 恩施州益寿果业股份有限公司 Preparation method of vitamin C-rich composite kiwi fruit powder
CN104082522A (en) * 2014-07-29 2014-10-08 哈尔滨伟平科技开发有限公司 Preparation method for healthcare instant soybean powder
CN104087637A (en) * 2014-07-29 2014-10-08 哈尔滨伟平科技开发有限公司 Method for preparing vegetable protein polypeptide by virtue of microbiological fermentation
CN104394319A (en) * 2014-11-24 2015-03-04 广州市浩云安防科技股份有限公司 Embedded HD network video recorder
CN105104978A (en) * 2015-09-08 2015-12-02 福建农林大学 Sprouting cereals flour and preparation method thereof

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107772142A (en) * 2016-08-26 2018-03-09 海盐县金利达饲料有限公司 A kind of feed grade fermentation rice meal rich in neutral proteinase and preparation method thereof
CN106722325A (en) * 2017-01-14 2017-05-31 哈尔滨伟平科技开发有限公司 The preparation method of chicken meal
CN106819956A (en) * 2017-01-14 2017-06-13 哈尔滨伟平科技开发有限公司 A kind of preparation method of shrimp med
CN106889466A (en) * 2017-01-15 2017-06-27 哈尔滨伟平科技开发有限公司 A kind of preparation method of compound nutritional soy meal
CN108283259A (en) * 2018-01-02 2018-07-17 中国农业科学院农产品加工研究所 Health-care instant corn meal and preparation method thereof
CN108283259B (en) * 2018-01-02 2021-04-27 中国农业科学院农产品加工研究所 Instant health sweet corn flour and its preparation method

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Application publication date: 20160817

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