CN105852104A - Preparation method of health-care instantly-dissolved protein powder - Google Patents
Preparation method of health-care instantly-dissolved protein powder Download PDFInfo
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- CN105852104A CN105852104A CN201610241962.5A CN201610241962A CN105852104A CN 105852104 A CN105852104 A CN 105852104A CN 201610241962 A CN201610241962 A CN 201610241962A CN 105852104 A CN105852104 A CN 105852104A
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- protein powder
- bacillus subtilis
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- seed liquor
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- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 21
- 102000004169 proteins and genes Human genes 0.000 title claims abstract description 21
- 239000000843 powder Substances 0.000 title claims abstract description 19
- 238000002360 preparation method Methods 0.000 title claims abstract description 7
- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 14
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 13
- 238000000855 fermentation Methods 0.000 claims abstract description 13
- 230000004151 fermentation Effects 0.000 claims abstract description 13
- 230000001954 sterilising effect Effects 0.000 claims abstract description 13
- 239000011268 mixed slurry Substances 0.000 claims abstract description 8
- 239000000203 mixture Substances 0.000 claims abstract description 8
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims abstract description 7
- 238000009835 boiling Methods 0.000 claims abstract description 7
- 239000000706 filtrate Substances 0.000 claims abstract description 7
- 239000008103 glucose Substances 0.000 claims abstract description 7
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 7
- 240000007594 Oryza sativa Species 0.000 claims abstract description 6
- 235000007164 Oryza sativa Nutrition 0.000 claims abstract description 6
- 235000009566 rice Nutrition 0.000 claims abstract description 6
- 240000008042 Zea mays Species 0.000 claims abstract description 5
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims abstract description 5
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims abstract description 5
- 235000005822 corn Nutrition 0.000 claims abstract description 5
- 240000004922 Vigna radiata Species 0.000 claims abstract description 4
- 235000010721 Vigna radiata var radiata Nutrition 0.000 claims abstract description 4
- 235000011469 Vigna radiata var sublobata Nutrition 0.000 claims abstract description 4
- 239000000084 colloidal system Substances 0.000 claims abstract description 4
- 238000000034 method Methods 0.000 claims abstract description 4
- 239000000047 product Substances 0.000 claims abstract description 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 6
- 239000007788 liquid Substances 0.000 claims description 6
- 239000001888 Peptone Substances 0.000 claims description 3
- 108010080698 Peptones Proteins 0.000 claims description 3
- 235000015278 beef Nutrition 0.000 claims description 3
- 239000001963 growth medium Substances 0.000 claims description 3
- 239000002054 inoculum Substances 0.000 claims description 3
- 235000019319 peptone Nutrition 0.000 claims description 3
- 239000011780 sodium chloride Substances 0.000 claims description 3
- 238000003756 stirring Methods 0.000 claims description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 3
- 108090000765 processed proteins & peptides Proteins 0.000 abstract description 11
- BTCSSZJGUNDROE-UHFFFAOYSA-N gamma-aminobutyric acid Chemical compound NCCCC(O)=O BTCSSZJGUNDROE-UHFFFAOYSA-N 0.000 abstract description 6
- 229960003692 gamma aminobutyric acid Drugs 0.000 abstract description 5
- 235000013305 food Nutrition 0.000 abstract description 4
- 150000001413 amino acids Chemical class 0.000 abstract description 3
- 230000036772 blood pressure Effects 0.000 abstract description 3
- 210000004556 brain Anatomy 0.000 abstract description 3
- 230000000694 effects Effects 0.000 abstract description 3
- 230000036039 immunity Effects 0.000 abstract description 3
- 238000012545 processing Methods 0.000 abstract description 2
- OGNSCSPNOLGXSM-UHFFFAOYSA-N (+/-)-DABA Natural products NCCC(N)C(O)=O OGNSCSPNOLGXSM-UHFFFAOYSA-N 0.000 abstract 1
- 238000001816 cooling Methods 0.000 abstract 1
- 239000004615 ingredient Substances 0.000 abstract 1
- 238000001694 spray drying Methods 0.000 abstract 1
- 238000005728 strengthening Methods 0.000 abstract 1
- 235000018102 proteins Nutrition 0.000 description 14
- 229920001184 polypeptide Polymers 0.000 description 9
- 102000004196 processed proteins & peptides Human genes 0.000 description 9
- 108010082495 Dietary Plant Proteins Proteins 0.000 description 7
- 208000001953 Hypotension Diseases 0.000 description 3
- 235000020776 essential amino acid Nutrition 0.000 description 3
- 239000003797 essential amino acid Substances 0.000 description 3
- 208000021822 hypotensive Diseases 0.000 description 3
- 230000001077 hypotensive effect Effects 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 2
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 2
- 244000046052 Phaseolus vulgaris Species 0.000 description 2
- 229920002494 Zein Polymers 0.000 description 2
- 235000021120 animal protein Nutrition 0.000 description 2
- 235000013339 cereals Nutrition 0.000 description 2
- 230000001256 tonic effect Effects 0.000 description 2
- 239000005019 zein Substances 0.000 description 2
- 229940093612 zein Drugs 0.000 description 2
- QWCKQJZIFLGMSD-UHFFFAOYSA-N 2-Aminobutanoic acid Natural products CCC(N)C(O)=O QWCKQJZIFLGMSD-UHFFFAOYSA-N 0.000 description 1
- QWCKQJZIFLGMSD-GSVOUGTGSA-N D-alpha-aminobutyric acid Chemical compound CC[C@@H](N)C(O)=O QWCKQJZIFLGMSD-GSVOUGTGSA-N 0.000 description 1
- 108010068370 Glutens Proteins 0.000 description 1
- 101100020212 Mus musculus Klhdc3 gene Proteins 0.000 description 1
- 108010064851 Plant Proteins Proteins 0.000 description 1
- 230000003556 anti-epileptic effect Effects 0.000 description 1
- 230000003276 anti-hypertensive effect Effects 0.000 description 1
- 239000001961 anticonvulsive agent Substances 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 210000004204 blood vessel Anatomy 0.000 description 1
- 230000002490 cerebral effect Effects 0.000 description 1
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 230000000295 complement effect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 230000003371 gabaergic effect Effects 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 238000009629 microbiological culture Methods 0.000 description 1
- 230000003020 moisturizing effect Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 235000021118 plant-derived protein Nutrition 0.000 description 1
- 210000000278 spinal cord Anatomy 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
- 230000001457 vasomotor Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/30—Working-up of proteins for foodstuffs by hydrolysis
- A23J3/32—Working-up of proteins for foodstuffs by hydrolysis using chemical agents
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/14—Vegetable proteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Nutrition Science (AREA)
- Virology (AREA)
- Biomedical Technology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Microbiology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- General Chemical & Material Sciences (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention belongs to the technical field of food processing, and particularly relates to a preparation method of health-care instantly-dissolved protein powder. The method comprises the steps that food-grade rice protein powder and food-grade corn protein powder are weighed and mixed, a glucose solution is added into the mixture and stirred to be uniform, sterilization is conducted, bacillus subtilis is inoculated for fermentation, boiling and cooling are conducted after fermentation is completed, and sterilized fermentation liquor is obtained; mung bean sprouts and the sterilized fermentation liquor are mixed and sent into a colloid mill, a mixed slurry is obtained, the mixed slurry is hydrolyzed and filtered, filtrate is obtained and subjected to concentrating and spray-drying, and the finished product is obtained. The health-care instantly-dissolved protein powder prepared through the method is rich in amino acid and small peptide, contains a large number of functional ingredients of gamma-aminobutyric acid and the like, is easy to absorb and has multiple effects of improving the organism immunity, lowering blood pressure, strengthening the brain and the like.
Description
Technical field
The invention belongs to food processing technology field, be specifically related to the preparation method of a kind of health instant protein powder.
Background technology
Protein is mainly derived from animal food and plant food, the essential amino acid A wide selection of colours and designs of animal protein, and ratio is reasonable, is therefore easier to digestion than general vegetable protein, absorbs and utilize, and nutritive value is the most relatively high.And vegetable protein is owing to lacking certain essential amino acid, the degree causing vegetable protein to be absorbed and utilized by the body also can differ from.The approach improving plant protein nutrition effect has two, and first,
By different types of vegetable protein mixed edible, such as mix edible by corn gluten protein and peas protein, then the two contained essential amino acid can be learnt from other's strong points to offset one's weaknesses, and is complementary to one another, can reach or close to the level of animal protein, the utilization rate of protein can be improved；Second, vegetable protein is degraded into small molecular protein polypeptide, after cheap vegetable protein is degraded into small molecular protein polypeptide, although the amino acid content of polypeptide is consistent with kind protein corresponding thereto, but polypeptide has its corresponding unexistent biologically actives of protein many, such as rice protein polypeptide can be hypotensive, promote fat metabolism, reduce serum cholesterol, rice protein does not then have this to act on, zein polypeptide has raising immunity of organisms, protect the liver, sober up, hypotensive grade acts on, and zein does not has this to act on.
Most plants polypeptide has the effect of suppression Angiotensin-Converting, thus reduces blood pressure, and Gabanergic acts on the vasomotor center of spinal cord, effectively facilitates blood vessel dilatation, reduces the purpose of blood pressure.GABA and vegetable protein polypeptide act synergistically, and can reach obvious antihypertensive effect, additionally, GABA also has brain tonic and intelligence development, anti-epileptic, promote sleep, moisturizing of improving looks, and delay cerebral senility to wait for a long time function.GABA is distributed widely in the seed of beans, cereal etc., and the beans after particularly germinateing and cereal, its alpha-aminobutyric acid content can increase several times to decades of times.
Summary of the invention
It is an object of the invention to provide the preparation method of a kind of health instant protein powder rich in the functional component such as polypeptide and GABA.
The object of the present invention is achieved like this:
Food-grade rice protein powder and food-grade corn albumen powder is weighed respectively according to the ratio of 1:1, mix, add the glucose solution of 1% concentration of egg mix 15-20 times of quality of white powder, stir, pH is adjusted to 6.8-7.2, sterilizing 20 minutes under the conditions of 121 DEG C, cool to 36-38 DEG C, inoculate bacillus subtilis seed liquor by the inoculum concentration of 5-10%, shaking table top fermentation 30-36 hour of 180-200rpm, 36-38 DEG C, be cooled to after then boiling 10 minutes 35-40 DEG C sterilization fermentation liquid.
Weigh the mung bean sprouts of certain mass, add the sterilization fermentation liquid of 10-15 times of quality, cross colloid mill and obtain mixed slurry, mixed slurry pH is adjusted to 4.8-5.2, maintain 4-5 hour under the conditions of 37 DEG C, then be warmed up to 50-55 DEG C, keep 2-4 hour, then filter to get filtrate after boiling 10 minutes, filtrate is the most concentrated, be spray-dried after get product.
Present invention additionally comprises following feature:
The preparation method of described bacillus subtilis seed liquor is: weighs peptone 5g, beef extract 3g, glucose 4g, sodium chloride 5g, adds water and be settled to 1000ml, pH is adjusted to 6.8-7.2, obtains bacillus subtilis seed liquor culture medium.Take 100ml seed liquor to cultivate based in 500ml triangular flask, 121 DEG C of sterilizings 20 minutes, be cooled to 36-38 DEG C, inoculate bacillus subtilis, the shaking table of 180-200rpm, 36-38 DEG C is cultivated 18-22 hour.
The health instant protein powder made by the inventive method, containing abundant amino acid and little peptide, simultaneously possibly together with functional components such as substantial amounts of GABAs, the most easily absorbs, and has the raising multiple efficacies such as immunity of organisms, hypotensive, brain tonic.
Specific embodiments
The Strains B. subtilis CICC21095 that the present invention uses purchases in Chinese industrial Microbiological Culture Collection administrative center.
Weigh peptone 5g, beef extract 3g, glucose 4g, sodium chloride 5g, add water and be settled to 1000ml, pH is adjusted to 7.0, obtain bacillus subtilis seed liquor culture medium.Take 100ml seed liquor to cultivate based in 500ml triangular flask, 121 DEG C of sterilizings 20 minutes, be cooled to 37 DEG C, inoculate bacillus subtilis, 190rpm, the shaking table of 37 DEG C are cultivated and within 20 hours, i.e. obtains bacillus subtilis seed liquor.
Food-grade rice protein powder and food-grade corn albumen powder is weighed respectively according to the ratio of 1:1, mix, add the glucose solution of 1% concentration of 18 times of quality of egg mix white powder, stir, pH is adjusted to 7.0, sterilizing 20 minutes under the conditions of 121 DEG C, cool to 37 DEG C, inoculum concentration by 8% inoculates bacillus subtilis seed liquor, in 190rpm, the shaking table top fermentation 33 hours of 37 DEG C, be cooled to after then boiling 10 minutes 37 DEG C sterilization fermentation liquid.
Weigh the mung bean sprouts of certain mass, add the sterilization fermentation liquid of 10 times of quality, cross colloid mill and obtain mixed slurry, mixed slurry pH is adjusted to 5.0, maintain 4.5 hours under the conditions of 37 DEG C, then be warmed up to 50 DEG C, keep 3 hours, then filter to get filtrate after boiling 10 minutes, filtrate is the most concentrated, be spray-dried after get product.
Claims (1)
1. a preparation method for health instant protein powder, is characterized in that being prepared from through following process:
(1) weigh peptone 5g, beef extract 3g, glucose 4g, sodium chloride 5g, add water and be settled to 1000ml, pH is adjusted to 6.8-7.2, obtains bacillus subtilis seed liquor culture medium, take 100ml seed liquor and cultivate based in 500ml triangular flask, 121 DEG C of sterilizings 20 minutes, it is cooled to 36-38 DEG C, inoculates bacillus subtilis, the shaking table of 180-200rpm, 36-38 DEG C is cultivated and within 18-22 hour, i.e. obtains bacillus subtilis seed liquor；
(2) weigh food-grade rice protein powder and food-grade corn albumen powder respectively according to the ratio of 1:1, mix, add the glucose solution of 1% concentration of egg mix 15-20 times of quality of white powder, stir, pH is adjusted to 6.8-7.2, sterilizing 20 minutes under the conditions of 121 DEG C, cool to 36-38 DEG C, bacillus subtilis seed liquor is inoculated by the inoculum concentration of 5-10%, shaking table top fermentation 30-36 hour of 180-200rpm, 36-38 DEG C, be cooled to after then boiling 10 minutes 35-40 DEG C sterilization fermentation liquid；
(3) weigh the mung bean sprouts of certain mass, add the sterilization fermentation liquid of 10-15 times of quality, cross colloid mill and obtain mixed slurry, mixed slurry pH is adjusted to 4.8-5.2, maintain 4-5 hour under the conditions of 37 DEG C, then be warmed up to 50-55 DEG C, keep 2-4 hour, then filter to get filtrate after boiling 10 minutes, filtrate is the most concentrated, be spray-dried after get product.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610241962.5A CN105852104A (en) | 2016-04-19 | 2016-04-19 | Preparation method of health-care instantly-dissolved protein powder |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610241962.5A CN105852104A (en) | 2016-04-19 | 2016-04-19 | Preparation method of health-care instantly-dissolved protein powder |
Publications (1)
| Publication Number | Publication Date |
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| CN105852104A true CN105852104A (en) | 2016-08-17 |
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Family Applications (1)
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| CN201610241962.5A Pending CN105852104A (en) | 2016-04-19 | 2016-04-19 | Preparation method of health-care instantly-dissolved protein powder |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106722325A (en) * | 2017-01-14 | 2017-05-31 | 哈尔滨伟平科技开发有限公司 | The preparation method of chicken meal |
| CN106819956A (en) * | 2017-01-14 | 2017-06-13 | 哈尔滨伟平科技开发有限公司 | A kind of preparation method of shrimp med |
| CN106889466A (en) * | 2017-01-15 | 2017-06-27 | 哈尔滨伟平科技开发有限公司 | A kind of preparation method of compound nutritional soy meal |
| CN107772142A (en) * | 2016-08-26 | 2018-03-09 | 海盐县金利达饲料有限公司 | A kind of feed grade fermentation rice meal rich in neutral proteinase and preparation method thereof |
| CN108283259A (en) * | 2018-01-02 | 2018-07-17 | 中国农业科学院农产品加工研究所 | Health-care instant corn meal and preparation method thereof |
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| CN105104978A (en) * | 2015-09-08 | 2015-12-02 | 福建农林大学 | Sprouting cereals flour and preparation method thereof |
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2016
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107772142A (en) * | 2016-08-26 | 2018-03-09 | 海盐县金利达饲料有限公司 | A kind of feed grade fermentation rice meal rich in neutral proteinase and preparation method thereof |
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| CN106819956A (en) * | 2017-01-14 | 2017-06-13 | 哈尔滨伟平科技开发有限公司 | A kind of preparation method of shrimp med |
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| CN108283259A (en) * | 2018-01-02 | 2018-07-17 | 中国农业科学院农产品加工研究所 | Health-care instant corn meal and preparation method thereof |
| CN108283259B (en) * | 2018-01-02 | 2021-04-27 | 中国农业科学院农产品加工研究所 | Instant health sweet corn flour and its preparation method |
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