CN105595022A

CN105595022A - Making method of dairy cow feed additives

Info

Publication number: CN105595022A
Application number: CN201610007390.4A
Authority: CN
Inventors: 吕庆茂
Original assignee: Harbin Weiping Technology Development Co Ltd
Current assignee: Harbin Weiping Technology Development Co Ltd
Priority date: 2016-01-07
Filing date: 2016-01-07
Publication date: 2016-05-25

Abstract

The invention belongs to the technical field of feed additives, and particularly relates to a making method of dairy cow feed additives. Bran, corn flour and pine needle meal are weighed and evenly mixed, water is added, sterilization is performed, russula vinosa seed liquid is inoculated after cooling, the mixture is placed into an artificial climate chamber to be cultured for 4 days and then is taken out, rhodotorula mucilaginosa seed liquid is inoculated, then the mixture is placed into the artificial climate chamber to continue to be cultured for 4 days, then fermented materials are dried and smashed, feed-level bile acid is added into the smashed materials, and the finished products are made through pelleting after even mixing. The dairy cow feed additives contain lots of carotene which can increase the milk yield of dairy cows, improve the quality of milk and the immunity of dairy cows and reduce diseases and the using amount of antibiotics, meanwhile, the dairy cow feed additives contain various digestive enzymes which can improve the digestive capacity of diary cows and further increase the milk yield.

Description

The preparation method of cow feed additive

Technical field

The invention belongs to field of feed additive technology, be specifically related to the preparation method of cow feed additive.

Background technology

Along with the development of Chinese society and the change of people's consumption habit, the consumption figure of dairy products increases year by year, and dairy products particularly milk become the important foodstuffs in people's daily life day by day. In recent years, along with improving constantly of living standards of the people, the requirement of the quality to dairy products is also more and more higher. In dairy cow diet, adding carotene preparation can increase the output of milk of milk cow, improve the quality of milk, butterfat percnetage and content of milk protein in milk are obviously increased, carrotene can improve the phagocytic activity of neutrocyte simultaneously, reduce the incidence of disease of mastadenitis of cow, reduce antibiotic consumption, avoid antibiotic residual and affect health in milk. At present, many carotene preparations come from artificial synthetic or biological extraction, and its cost is higher, expensive, and its application is greatly limited.

Summary of the invention

The object of this invention is to provide a kind of low cost, be rich in the preparation method of the cow feed additive of carrotene and multiple digestive ferment.

The object of the present invention is achieved like this:

Take 5 grams of sucrose, 1 gram of peptone, 0.15 gram, urea, 0.1 gram of potassium dihydrogen phosphate, 0.05 gram, magnesium sulfate, adds 100 milliliters of clear water stirring and dissolving, sterilizing 20 minutes at 121 DEG C, be cooled to 28～30 DEG C, inoculation rhodotorula mucilaginosa is cultivated and within 20～24 hours, is obtained rhodotorula mucilaginosa seed liquor under 28～30 DEG C, the condition of rotary shaker 160～200rpm.

Take 3 grams of pine needle meals, 2 grams of maltose, 1 gram, wheat bran, 0.2 gram of potassium dihydrogen phosphate, 0.15 gram, magnesium sulfate, 0.03 gram, ferrous sulfate, join in 100 ml waters, stir, 121 DEG C of sterilizings 20 minutes, are cooled to 26～28 DEG C, access Russula vinosa inclined-plane seed, at 26～28 DEG C, under rotary shaker 180～200rpm condition, cultivate and within 7～9 days, obtain Russula vinosa seed liquor.

Take respectively wheat bran according to the ratio of mass ratio 5:4:1, corn flour and pine needle meal, after mixing, add water, it is 60～65% that water content is controlled at, under 121 DEG C of conditions, sterilizing is after 30 minutes, be cooled to 26～28 DEG C, the Russula vinosa seed liquor of access 5%, mix thoroughly, tiling is to tray, 4～6 centimetres of thickness, cover 6 layers of sterile gauze above, putting into growth cabinet cultivates, 26～28 DEG C of cultivation temperature, growth cabinet relative humidity 85～90%, cultivate and take out afterwards for 4 days, the rhodotorula mucilaginosa seed liquor of access 5%, mix thoroughly, again tile on tray, 4～6 centimetres of thickness, cover 6 layers of sterile gauze above, putting into growth cabinet cultivates, 28 DEG C of cultivation temperature, growth cabinet relative humidity 90%, continue to cultivate was dried to moisture below 10% by fermentation material after 4 days under 50 DEG C of conditions, then pulverize, in crushed material, add 0.1% feed grade bile acid, 2～4% feed grade fatty powder, after mixing, get product through granulation.

Cow feed additive of the present invention, contain a large amount of carrotene that Russula vinosa and rhodotorula mucilaginosa metabolism produce, can increase the output of milk of milk cow, improve the quality of milk, improve the immunity of milk cow, reduce the generation of disease, reduce antibiotic consumption, also multiple digestive enzymes of this cow feed additive simultaneously, comprise cellulase, amylase, protease, lipase, phytase etc., these enzymes with difference in functionality can will be difficult for the cellulose of degraded in feed, carbohydrate, protein, fat etc. is degraded into the small-molecule substance easily being absorbed by milk cow, improve the digestion power of milk cow and further improve the output of milk. when feeding cow, feed addictive of the present invention is joined in cow concentrated feed and mixed in 0.8～1.2% ratio.

Specific embodiments

The bacterial strain rhodotorula mucilaginosa CICC31029 that the present invention adopts is purchased from Chinese industrial microorganism fungus kind preservation administrative center, and Russula vinosa CFCC86931 is purchased from China Forest microorganism fungus kind preservation administrative center.

Take 5 grams of sucrose, 1 gram of peptone, 0.15 gram, urea, 0.1 gram of potassium dihydrogen phosphate, 0.05 gram, magnesium sulfate, adds 100 milliliters of clear water stirring and dissolving, sterilizing 20 minutes at 121 DEG C, be cooled to 29 DEG C, inoculation rhodotorula mucilaginosa is cultivated and within 22 hours, is obtained rhodotorula mucilaginosa seed liquor under 29 DEG C, the condition of rotary shaker 180rpm.

Take 3 grams of pine needle meals, 2 grams of maltose, 1 gram, wheat bran, 0.2 gram of potassium dihydrogen phosphate, 0.15 gram, magnesium sulfate, 0.03 gram, ferrous sulfate, join in 100 ml waters, stir, 121 DEG C of sterilizings 20 minutes, are cooled to 27 DEG C, access Russula vinosa inclined-plane seed, at 27 DEG C, under rotary shaker 190rpm condition, cultivate and within 8 days, obtain Russula vinosa seed liquor.

Take respectively wheat bran according to the ratio of mass ratio 5:4:1, corn flour and pine needle meal, after mixing, add water, it is 65% that water content is controlled at, under 121 DEG C of conditions, sterilizing is after 30 minutes, be cooled to 27 DEG C, the Russula vinosa seed liquor of access 5%, mix thoroughly, tiling is to tray, 5 centimetres of thickness, cover 6 layers of sterile gauze above, putting into growth cabinet cultivates, 27 DEG C of cultivation temperature, growth cabinet relative humidity 85%, cultivate and take out afterwards for 4 days, the rhodotorula mucilaginosa seed liquor of access 5%, mix thoroughly, again tile on tray, 5 centimetres of thickness, cover 6 layers of sterile gauze above, putting into growth cabinet cultivates, 28 DEG C of cultivation temperature, growth cabinet relative humidity 90%, continue to cultivate was dried to moisture below 10% by fermentation material after 4 days under 50 DEG C of conditions, then pulverize, in crushed material, add 0.1% feed grade bile acid, 3% feed grade fatty powder, after mixing, get product through granulation.

Claims

1. the preparation method of cow feed additive, is characterized in that being prepared from through following process:

(1) take 5 grams of sucrose, 1 gram of peptone, 0.15 gram, urea, 0.1 gram of potassium dihydrogen phosphate, 0.05 gram, magnesium sulfate, adds 100 milliliters of clear water stirring and dissolving, sterilizing 20 minutes at 121 DEG C, be cooled to 28～30 DEG C, inoculation rhodotorula mucilaginosa is cultivated and within 20～24 hours, is obtained rhodotorula mucilaginosa seed liquor under 28～30 DEG C, the condition of rotary shaker 160～200rpm;

(2) take 3 grams of pine needle meals, 2 grams of maltose, 1 gram, wheat bran, 0.2 gram of potassium dihydrogen phosphate, 0.15 gram, magnesium sulfate, 0.03 gram, ferrous sulfate, join in 100 ml waters, stir, 121 DEG C of sterilizings 20 minutes, are cooled to 26～28 DEG C, access Russula vinosa inclined-plane seed, at 26～28 DEG C, under rotary shaker 180～200rpm condition, cultivate and within 7～9 days, obtain Russula vinosa seed liquor;

(3) take respectively wheat bran according to the ratio of mass ratio 5:4:1, corn flour and pine needle meal, after mixing, add water, it is 60～65% that water content is controlled at, under 121 DEG C of conditions, sterilizing is after 30 minutes, be cooled to 26～28 DEG C, the Russula vinosa seed liquor of access 5%, mix thoroughly, tiling is to tray, 4～6 centimetres of thickness, cover 6 layers of sterile gauze above, putting into growth cabinet cultivates, 26～28 DEG C of cultivation temperature, growth cabinet relative humidity 85～90%, cultivate and take out afterwards for 4 days, the rhodotorula mucilaginosa seed liquor of access 5%, mix thoroughly, again tile on tray, 4～6 centimetres of thickness, cover 6 layers of sterile gauze above, putting into growth cabinet cultivates, 28 DEG C of cultivation temperature, growth cabinet relative humidity 90%, continue to cultivate was dried to moisture below 10% by fermentation material after 4 days under 50 DEG C of conditions, then pulverize, in crushed material, add 0.1% feed grade bile acid, 2～4% feed grade fatty powder, after mixing, get product through granulation.