CN107494929A - A kind of pure natural feed addictive of layer chicken and preparation method thereof - Google Patents
A kind of pure natural feed addictive of layer chicken and preparation method thereof Download PDFInfo
- Publication number
- CN107494929A CN107494929A CN201710941750.2A CN201710941750A CN107494929A CN 107494929 A CN107494929 A CN 107494929A CN 201710941750 A CN201710941750 A CN 201710941750A CN 107494929 A CN107494929 A CN 107494929A
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- CN
- China
- Prior art keywords
- feed addictive
- thalline
- rhodotorula
- wheat bran
- acid treatment
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/70—Feeding-stuffs specially adapted for particular animals for birds
- A23K50/75—Feeding-stuffs specially adapted for particular animals for birds for poultry
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Abstract
The invention discloses a kind of preparation method of pure natural feed addictive of layer chicken, belong to biofermentation and feed additive field.The present invention includes the steps such as liquid fermentation Rhodotorula mucilaginose, thalline acid treatment, the mixing of wheat bran zymotic fluid, mixing drying；The present invention uses liquid fermentation rhodotorula mucilaginosa, using acetum pyrolignosum rectificatum liquid acid treatment rhodotorula mucilaginosa cell, cracks its cell so that the active ingredient such as pigment in cell fully discharges；Simultaneously using the antifungal substance in the natural disinfection function and rhodotorula mucilaginosa zymotic fluid of acetum pyrolignosum rectificatum liquid, suppress the growth of harmful bacteria, so as to substantially increase the content of pigment content and antibacterial material in feed additives products.
Description
Technical field
The invention belongs to biofermentation and feed additive field, is related to a kind of preparation of pure natural feed addictive of layer chicken
Method.
Background technology
With the food-safe great attention of consumer, bigger challenge and opportunity are proposed to feed industry, feed adds
Agent is added to turn into whole industry focus of attention, countries in the world all make in the feed addictive such as numerous and confused disabling or limitation antibiotic
With.
Natural feed addictive is the development trend of current feed industry, can either in supplement feed the deficiency of nutritional ingredient or
Person protects the nutritional ingredient in feed, and environment will not be polluted again；It is to realize green cultivation, ensure that animal husbandry continues health
Development, the fundamental way for ensureing human health.
Efficient, natural feed addictive is developed so needing badly at present.
The content of the invention
The technical problems to be solved by the invention are to provide a kind of preparation method of pure natural feed addictive of layer chicken, with gram
The shortcomings of existing feed addictive function is single is taken, has the feed addition of " natural antibiotics " and " natural pigment " dual-use function concurrently
Agent.
In order to solve the above technical problems, the technical solution used in the present invention is：A kind of pure natural feed addictive of layer chicken
Preparation method, it is characterised in that comprise the following steps：
(1) liquid fermentation Rhodotorula mucilaginose
By the bacteria suspension that fermented to obtain in the Rhodotorula mucilaginose liquid medium within of activation, separated through bacterium solution, obtain rhodotorula mucilaginosa
Thalline and zymotic fluid；
(2) thalline acid treatment
Acid treatment is carried out to step (1) the rhodotorula mucilaginosa thalline with acetum pyrolignosum rectificatum liquid, destroys cell, is obtained at thalline acid
Manage thing；
(3) wheat bran zymotic fluid mixes
Wheat bran is added in step (1) described zymotic fluid, obtains wheat bran fermentation liquid mixture；
(4) mixing drying
Thalline acid treatment thing described in step (2), (3), wheat bran fermentation liquid mixture are sufficiently mixed, dries, obtains
Obtain feed addictive of layer chicken.
Further technical scheme is that step (1) separates Rhodotorula mucilaginose body and zymotic fluid using centrifugation mode,
Centrifugal rotational speed be the 4000-10000r/min. centrifugal treating times be 1-10min.
Further technical scheme is that the incubation of step (1) is that the Rhodotorula mucilaginose of activation is inoculated in into pH value
For in 3-6 PDA liquid medium, bacteria suspension is obtained after cultivating 24-180h at 25-35 DEG C；Wherein inoculum density is 0.1-
1%.
Further technical scheme is that step (2) carries out rhodotorula mucilaginosa thalline used and acetum pyrolignosum rectificatum liquid during acid treatment
Volume ratio be 1:5-10；Carry out acid treatment time 10-30min.
Further technical scheme is, zymotic fluid and wheat bran weight used in step (3) the wheat bran fermentation liquid mixture
Than for 1:1-3.
Further technical scheme is that the drying temperature of step (4) is 45 DEG C.
It is using beneficial effect caused by above-mentioned technical proposal：
(1) method of the invention for preparing feed addictive uses liquid fermentation rhodotorula mucilaginosa, at acetum pyrolignosum rectificatum liquid acid
Rhodotorula mucilaginosa cell is managed, cracks its cell so that carotenoid active ingredient fully discharges in cell.
(2) feed addictive prepared by the present invention utilizes the natural disinfection function and rhodotorula mucilaginosa zymotic fluid of acetum pyrolignosum rectificatum liquid
In antifungal substance, suppress the growth of harmful bacteria, so as to substantially increase pigment content and antibacterial in feed additives products
The content of material.
(3) the pure natural feed addictive of layer chicken that the inventive method is prepared, has " natural pigment " and " natural antibiosis concurrently
Element " dual-use function, safety and environmental protection, it is a kind of new, green, efficient natural feed addictive of layer chicken.
Embodiment
The technical scheme in the embodiment of the present invention is clearly and completely described below, it is clear that described embodiment
Only part of the embodiment of the invention, rather than whole embodiments.It is common based on the embodiment in the present invention, this area
The every other embodiment that technical staff is obtained under the premise of creative work is not made, belong to the model that the present invention protects
Enclose.
Many details are elaborated in the following description to facilitate a thorough understanding of the present invention, still the present invention can be with
It is different from other manner described here using other to implement, those skilled in the art can be without prejudice to intension of the present invention
In the case of do similar popularization, therefore the present invention is not limited by following public specific embodiment.
The invention provides a kind of preparation method of pure natural feed addictive of layer chicken, it is characterised in that including following step
Suddenly：
(1) liquid fermentation Rhodotorula mucilaginose
By the bacteria suspension that fermented to obtain in the Rhodotorula mucilaginose liquid medium within of activation, separated through bacterium solution, obtain rhodotorula mucilaginosa
Thalline and zymotic fluid；
(2) thalline acid treatment
Acid treatment is carried out to step (1) the rhodotorula mucilaginosa thalline with acetum pyrolignosum rectificatum liquid, destroys cell, is obtained at thalline acid
Manage thing；
(3) wheat bran zymotic fluid mixes
Wheat bran is added in step (1) described zymotic fluid, obtains wheat bran fermentation liquid mixture；
(4) mixing drying
Thalline acid treatment thing described in step (2), (3), wheat bran fermentation liquid mixture are sufficiently mixed, dries, obtains
Obtain feed addictive of layer chicken.
Preferably, step (1) separates Rhodotorula mucilaginose body and zymotic fluid using centrifugation mode, and centrifugal rotational speed is
4000-10000r/min. the centrifugal treating time is 1-10min.
Preferably, the incubation of step (1) is that the Rhodotorula mucilaginose of activation is inoculated in into the PDA liquid that pH value is 3-6
In culture medium, bacteria suspension is obtained after cultivating 24-180h at 25-35 DEG C；Wherein inoculum density is 0.1-1%.
Preferably, it is 1 that step (2), which carries out the volume ratio of rhodotorula mucilaginosa thalline used and acetum pyrolignosum rectificatum liquid during acid treatment,:5-
10；Carry out acid treatment time 10-30min.
Preferably, zymotic fluid and wheat bran weight ratio are 1 used in step (3) the wheat bran fermentation liquid mixture:1-3.
Preferably, the drying temperature of step (4) is 45 DEG C.
Embodiment 1
(1) liquid fermentation Rhodotorula mucilaginose
By the Rhodotorula mucilaginose inoculation 0.1% of activation in the PDA liquid medium that pH value is 3.5,25 DEG C of fermented and cultureds
180h, obtain bacteria suspension；The 4000r/min centrifugal treating times are 10min, bacterium solution separation, obtain rhodotorula mucilaginosa thalline and zymotic fluid；
(2) thalline acid treatment
By rhodotorula mucilaginosa thalline：Acetum pyrolignosum rectificatum liquid proportional is 1:5 handle rhodotorula mucilaginosa thalline 30min with acetum pyrolignosum rectificatum, break
Bad cell, obtain thalline acid treatment thing；
(3) wheat bran zymotic fluid mixes
By zymotic fluid and wheat bran 1:1 ratio, wheat bran is added in zymotic fluid, obtain wheat bran fermentation liquid mixture；
(4) mixing drying
Thalline acid treatment thing described in step (2), (3), wheat bran fermentation liquid mixture are sufficiently mixed, 45 DEG C of bakings
It is dry, obtain feed addictive of layer chicken.
Embodiment 2
(1) liquid fermentation Rhodotorula mucilaginose
By the Rhodotorula mucilaginose inoculation 0.5% of activation in the PDA liquid medium that pH value is 4.5,28 DEG C of fermented and cultureds
150h, obtain bacteria suspension；The 6000r/min centrifugal treating times are 8min, bacterium solution separation, obtain rhodotorula mucilaginosa thalline and zymotic fluid；
(2) thalline acid treatment
By thalline：Acetum pyrolignosum rectificatum liquid proportional is 1:8 handle rhodotorula mucilaginosa thalline 20min with acetum pyrolignosum rectificatum liquid, destroy cell,
Obtain thalline acid treatment thing；
(3) wheat bran zymotic fluid mixes
By zymotic fluid and wheat bran 1:2 ratio, wheat bran is added in zymotic fluid, obtain wheat bran fermentation liquid mixture；
(4) mixing drying
Thalline acid treatment thing described in step (2), (3), wheat bran fermentation liquid mixture are sufficiently mixed, 45 DEG C of bakings
It is dry, obtain feed addictive of layer chicken.
Embodiment 3
(1) liquid fermentation Rhodotorula mucilaginose
By the Rhodotorula mucilaginose inoculation 1% of activation in the PDA liquid medium that pH value is 5.5,35 DEG C of fermented and cultureds
24h, obtain bacteria suspension；The 10000r/min centrifugal treating times are 3min, bacterium solution separation, obtain rhodotorula mucilaginosa thalline and zymotic fluid；
(2) thalline acid treatment
By rhodotorula mucilaginosa thalline：Acetum pyrolignosum rectificatum liquid proportional is 1:10 handle rhodotorula mucilaginosa thalline 10min with acetum pyrolignosum rectificatum liquid,
Cell is destroyed, obtains thalline acid treatment thing；
(3) wheat bran zymotic fluid mixes
By zymotic fluid and wheat bran 1:3 ratio, wheat bran is added in zymotic fluid, obtain wheat bran fermentation liquid mixture；
(4) mixing drying
Thalline acid treatment thing described in step (2), (3), wheat bran fermentation liquid mixture are sufficiently mixed, 45 DEG C of bakings
It is dry, obtain feed addictive of layer chicken.
Contrast test
With common feed as a control group, feed addictive described in embodiment 1-3 point is added with common feed respectively
Zuo Wei not example 1-3；
Feed described in control group, example 1-3 is tested on breeding layer chicken：Under equal ambient, body is utilized
Function identical laying hen carries out check experiment, and under other identical implementation processes, effect is as shown in the table：
Pyrolkigneous liquid, also referred to as plant acid, be the byproduct of carbonization of wood process, be one kind based on acetic acid, containing alcohol, phenol,
The mixture of many kinds of substance such as ketone, aldehyde and its derivative.Present invention applicant by refined pyrolkigneous liquid feed addictive poultry,
Applied in livestock cultivation, there is sterilization, anti-inflammatory, expelling parasite, kill the virus, permeate, suppressing and activating cell etc. acts on.Given birth to improving
Performance is produced, the effect of strengthen immunity.
Rhodotorula mucilaginosa (Rhodotorula mucilaginosa) is eucaryote, Rhodotorula.Present invention applicant grinds
Study carefully discovery, rhodotorula mucilaginosa produces carotenoid, and peanut oil, apricot kernel oil and vitamin B, C etc., which have, promotes rhodotorula mucilaginosa pigment to increase
The effect of production, rhodotorula mucilaginosa can improve performance in layers, immunocompetence and resistance against diseases.Carotenoid in rhodotorula mucilaginosa
In breeding layer chicken, laying hen yolk color can be improved, improve Egg Quality.It there is no at present with " natural antibiotics --- acetum pyrolignosum rectificatum
The feed additives products of liquid " and " natural pigment --- rhodotorula mucilaginosa carotenoid " dual-use function.Therefore, the present invention is to raising
Expect that industry is significant.
Claims (7)
1. a kind of preparation method of pure natural feed addictive of layer chicken, it is characterised in that comprise the following steps：
(1) liquid fermentation Rhodotorula mucilaginose
By the bacteria suspension that fermented to obtain in the Rhodotorula mucilaginose liquid medium within of activation, separated through bacterium solution, obtain rhodotorula mucilaginosa thalline
And zymotic fluid；
(2) thalline acid treatment
Acid treatment is carried out to step (1) the rhodotorula mucilaginosa thalline with acetum pyrolignosum rectificatum liquid, destroys cell, obtains thalline acid treatment
Thing；
(3) wheat bran zymotic fluid mixes
Wheat bran is added in step (1) described zymotic fluid, obtains wheat bran fermentation liquid mixture；
(4) mixing drying
Thalline acid treatment thing described in step (2), (3), wheat bran fermentation liquid mixture are sufficiently mixed, dries, obtains egg
Chicken feed addictive.
A kind of 2. preparation method of pure natural feed addictive of layer chicken according to claim 1, it is characterised in that step
(1) Rhodotorula mucilaginose body and zymotic fluid are separated using centrifugation mode, centrifugal rotational speed is at 4000-10000r/min. centrifugations
The reason time is 1-10min.
A kind of 3. preparation method of pure natural feed addictive of layer chicken according to claim 1, it is characterised in that step
(1) incubation is to be inoculated in the Rhodotorula mucilaginose of activation in the PDA liquid medium that pH value is 3-6, at 25-35 DEG C
Bacteria suspension is obtained after culture 24-180h；Wherein inoculum density is 0.1-1%.
A kind of 4. preparation method of pure natural feed addictive of layer chicken according to claim 1, it is characterised in that step
(2) volume ratio of rhodotorula mucilaginosa thalline used and acetum pyrolignosum rectificatum liquid is 1 when carrying out acid treatment:5-10；Carry out acid treatment time 10-
30min。
A kind of 5. preparation method of pure natural feed addictive of layer chicken according to claim 1, it is characterised in that step
(3) zymotic fluid and wheat bran weight ratio are 1 used in the wheat bran fermentation liquid mixture:1-3.
A kind of 6. preparation method of pure natural feed addictive of layer chicken according to claim 1, it is characterised in that step
(4) drying temperature is 45 DEG C.
A kind of 7. pure natural feed addictive of layer chicken that method as described in claim any one of 1-6 is prepared.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710941750.2A CN107494929A (en) | 2017-10-11 | 2017-10-11 | A kind of pure natural feed addictive of layer chicken and preparation method thereof |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710941750.2A CN107494929A (en) | 2017-10-11 | 2017-10-11 | A kind of pure natural feed addictive of layer chicken and preparation method thereof |
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| CN107494929A true CN107494929A (en) | 2017-12-22 |
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| CN201710941750.2A Pending CN107494929A (en) | 2017-10-11 | 2017-10-11 | A kind of pure natural feed addictive of layer chicken and preparation method thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108185125A (en) * | 2018-02-12 | 2018-06-22 | 平南县亿翔环保能源有限公司 | A kind of wood vinegar feed addictive for breeding layer chicken |
| CN108522794A (en) * | 2018-03-23 | 2018-09-14 | 哈尔滨伟平科技开发有限公司 | A kind of preparation method of feed addictive of layer chicken |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108185125A (en) * | 2018-02-12 | 2018-06-22 | 平南县亿翔环保能源有限公司 | A kind of wood vinegar feed addictive for breeding layer chicken |
| CN108522794A (en) * | 2018-03-23 | 2018-09-14 | 哈尔滨伟平科技开发有限公司 | A kind of preparation method of feed addictive of layer chicken |
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Application publication date: 20171222 |
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