CN103583795A - Microbial fermentation bean dreg feed - Google Patents
Microbial fermentation bean dreg feed Download PDFInfo
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- CN103583795A CN103583795A CN201210290529.2A CN201210290529A CN103583795A CN 103583795 A CN103583795 A CN 103583795A CN 201210290529 A CN201210290529 A CN 201210290529A CN 103583795 A CN103583795 A CN 103583795A
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Abstract
The invention relates to microbial fermentation bean dreg feed. The feed is prepared by inoculation of a bean dreg mixture to a microbial mother liquor and fermentation. Based on one ton of the feed, strains of the microbial mother liquor comprise 400-450 mL of saccharomycetes, 150-250 mL of bacillus coagulans, 300-400 mL of lactobacillus acidophilus and 10-100 mL of an acetobacter. The bean dreg mixture per ton comprises following materials by weight: 14-34% of bean dreg, 10.7-18.7% of sesame powder, 10-18% of dandelion powder, 0.3-0.6% of sodium chloride, 0.7-1.0% of ammonium bicarbonate and 40-52% of water. According to the bean dreg feed, a portion of other plant pigment extraction offal is added into the bean dreg left after pigment extraction, and converted into the feed for pigs, chickens, goose, and the like by microbial fermentation. The feed can improve feed palatability, increase enzyme components in the feed, and reduce the feed cost. The feed has reasonable formula design, simple operation and manufacturing convenience, and is suitable for being used by farmers and breeding factories.
Description
Technical field
The invention belongs to technical field of biological fermentation, relate to a kind of animal feed, relate in particular to bean dregs feed of a kind of microorganism fermentation and preparation method thereof.
Background technology
Bean dregs are byproducts of producing in soymilk or bean curd process, and the output of annual global bean dregs is all very large.China is the cradle that bean curd is produced, and has long bean curd production history, and the producing and selling amount of bean curd is all larger, and corresponding bean dregs output is also very large.Along with the reach of science, the raising of human culture quality, people start to re-recognize soybean residue from the angle of nutrition.Prove after deliberation, in soybean, some nutritional labeling remains in bean dregs, general soybean residue water content 85%,
protein3.0%,
fat0.5%,
carbohydrate(cellulose, polysaccharide etc.) 8.0%, in addition, also contain the mineral matters such as calcium, phosphorus, iron.
At present lower to the utilization rate of bean dregs, traditional mode of utilizing is using bean dregs as animal feed, direct nutrition purposes, especially for feeding animals, and in bean dregs, the components utilising rate of a lot of high nutritive values is very low.In Japan, a large amount of bean dregs are incinerated.In Hong Kong, bean dregs are stacked usually used as discarded object, do not do any utilization.The visible bean dregs that how to utilize better become the problem of being badly in need of research and solving.
Summary of the invention
In order to achieve the above object, the present invention after soybean is extracted add other phytochrome of part to extract offal in remaining bean dregs, and by the microorganism feeds such as being translated into pig, chicken, goose that ferments, improve feed palatability, increase feed enzymes composition, reduce the cost of concentrated feed, improve efficiency of feed utilization and nutritive value, reach and save food, the object of resource circulation utilization.
The technical solution adopted for the present invention to solve the technical problems is:
The bean dregs feed of microorganism fermentation, is characterized in that it is through fermentation, to make feed by inoculate bean dregs mixture in microorganism mother liquor;
The bacterial classification that wherein said microorganism mother liquor is selected, by the proportioning of feedstuff per ton is: saccharomycete 400-450 milliliter, bacillus coagulans 150-250 milliliter, lactobacillus acidophilus 300-400 milliliter, acetic acid bacteria 10-100 milliliter;
Wherein said bean dregs mixture by weight proportion per ton is: bean dregs 14-34%, black sesame powder 10.7-18.7%, dandelion powder 10-18%, sodium chloride 0.3-0.6%, carbonic hydroammonium 0.7-1.0%, water 40-52%.
The preparation method of the bean dregs feed of microorganism fermentation of the present invention is to be undertaken by following step:
First carry out slant strains cultivation, then through the cultivation of liquid one-level shake-flask seed, liquid two stage, cultivate, bacterial classification is combined according to the above ratio and makes microorganism mother liquor, be then inoculated into bean dregs mixing species, process by fermentation, make fermenting bean dregs feed.
The culture medium of the present invention's bacterial classification used and the corresponding use of bacterial classification sees the following form:
Table 1
| Sequence number | Bacterium name | Numbering | Culture medium |
| 1 | Saccharomycete | AS1.1226 | Peptone 1%, beef extract 0.5%, sodium chloride 0.5%, pH7.2-7.4 |
| 2 | Bacillus coagulans | IFFI10067 | Peptone 1%, beef extract 0.5%, sodium chloride 0.5%, pH7.2-7.4 |
| 3 | Lactobacillus acidophilus | IFFI06005 | Skimmed milk power 10%, pH6.0 |
| 4 | Acetic acid bacteria | AS2.120 | Peptone 2%, yeast extract 1%, glucose 2%, pH5.5 |
Slant strains of the present invention is cultivated: each bacterial classification is used corresponding culture medium and adds 1.5-2.5% agar, inoculum concentration 5-10%, and under 25-35 ℃ of condition, static cultivation 20-48 hour, puts into 4 ℃ of Refrigerator stores; Described shaking flask condition of culture: temperature 25-40 ℃, rotating speed 80-180 beat/min, inoculum concentration 1-3%, cultivates 19-30h.
The bacterial classification that microorganism mother liquor of the present invention is selected is by the proportioning of feedstuff per ton, to form: saccharomycete 400-450 milliliter, bacillus coagulans 150-250 milliliter, lactobacillus acidophilus 300-400 milliliter, acetic acid bacteria 20-100 milliliter;
Fermenting bean dregs feed of the present invention forms by weight proportion per ton: bean dregs 14-34%, black sesame powder 10.7-18.7%, dandelion powder 10-18%, sodium chloride 0.3-0.6%, carbonic hydroammonium 0.7-1.0%, water 40-52%.
Fermenting bean dregs finished feed proterties of the present invention: color is isabelline strong acid fragrant and chrysanthemum fragrance and ester fragrance, quality softness, palatability is strong.
Fermenting bean dregs feed performance of the present invention: the nutritional labeling of having improved raw material, make raw material pH value 2.0 bring up to 4.5-5.0, wherein thick protein is brought up to 15-17%, organic acid content reaches 1.4-1.8%, and 70-80% is Pfansteihl, can directly substitutes bran feed and be absorbed by livestock.
By the poultry to different, bird respectively control group and experimental group test nursing, with the experiment of fermenting bean dregs feed, feed.Feeding effect sees the following form 2:
Table 2
| The growing and fattening pigs daily output (kilogram) | Broiler chicken (jin) | Fish (jin) | |
| Control group | 0.23 | 1.45 | 2.57 |
| Test group | 0.35 | 1.96 | 3.83 |
The present invention meets standard GB/T/T6432-94 after testing; GB/T6433-94; GB/T6434-94; GB/T6436-92; GB/T6437-92; GB/T6438-92; GB/T6435-86; Wherein detect total moisture: 54.4%; Thick protein: 16.5%; Crude fat: 8.70%; Coarse ash: 8.76%; Total phosphorus: 0.349%; Calcium: 0.62%; Crude fibre; 6.85%.
Reasonable recipe of the present invention, preparation method science, the scope of application is extensive, simple to operate, easily grasps, and cost is low, and feedstuff-meat ratio is low, and dressing percentage is high, and nursing egg bird laying rate is high.And have and strengthen the disease-resistant effect of livestock and poultry.The present invention has following characteristics:
1, in fermenting bean dregs feed, contain multiple natural colouring matter, as anthocyanidin, and anthocyanidin is jinx's delaying sanility of oxygen radical and the effect that increases immunologic function.Black sesame powder is the leftover bits and pieces after lactobacillus-fermented extracts its pigment by fresh sesame, so be rich in natural yellow carotenoid, as feed, can make the jaundice of the poultry colour of skin, delicious meat.
2, in fermenting bean dregs feed, containing a large amount of digestive ferments comprises: amylase, pectase, 1,4 beta-glucanase etc., by above-mentioned enzyme material hydrolysis, by Partial digestions such as starch, pectin, beta glucans in stalk, improve the nutritive value of feed, and can make nutritional labeling enter into the digestive system of livestock, assist the digestion of livestock endogenous enzymes, directly improved digesting and assimilating of feed, in addition, can effectively reduce the viscosity of enteron aisle Dissolve things inside, prevent from stopping up intestinal cell fenestra, promote transmission and the absorption of nutriment, improve efficiency of feed utilization;
3, the microbial cells that contains the above-mentioned digestive ferment of a large amount of secretions in fermenting bean dregs feed, can be colonizated in livestock alimentary canal, for livestock provides a large amount of digestive ferments and improves the more efficiently utilization rate that improves feed of environment in alimentary canal.
4, in fermenting bean dregs feed, contain and have in a large number acid-producing bacteria, can produce the softening stalk of a large amount of organic acids and improve palatability, the concentrated feed after fermentation is fragrant and sesame fragrance and ester fragrance with acid, stimulates livestock appetite, increases feed intake.Acid can reduce pH value and suppress various spoilage organisms growths, extends the shelf-life of feed.Can stimulate digestion liquid secretion of the Pfansteihl producing, promotes gastrointestinal peristalsis, slows down gastrointestinal emptying speed, makes nutriment have digestion time more fully, improves digestibility.
5, fermenting bean dregs feed contains multiple probio, is added to pasture in feed, can improve micro-ecological environment in livestock body, suppresses the growth of pathogenic bacteria in livestock body, improves physique and the resistance against diseases of livestock, obviously extends livestock production capacity and time.
Fermenting bean dregs feed safety of the present invention is nontoxic, and the strong adaptability of fermentation condition can ferment under the condition of 10-40 ℃ of yeasting, and fermenting speed is directly proportional to ambient temperature, and 20 ℃ with top fermentation 7-10 days, 15 ℃ of fermentation 10-15 days.
Fermenting bean dregs feed quality of the present invention is soft, palatability is strong, and moisture reaches 40-60%, and aromatic flavour contains a large amount of natural colouring matters and has nutrition and health care function.Formula Design is reasonable, simple to operate, easy to make, suitable wide farmers and cultivation factory, and fermented feed has improved wherein moisture 54.4% of nutritional labeling; Thick protein: 16.5%; Crude fat: 8.70%; Coarse ash: 8.76%; Total phosphorus: 0.349%; Calcium: 0.62%; Crude fibre; 6.85%; Organic acid content reaches 1.4-1.8%, and 70-80% is Pfansteihl, can directly by livestock, be absorbed.The feeding effect of fermented feed is fine, and feed palatability is better, and the digestibility of feed improves, and daily gain and meat feed ratio increase, and livestock resistance against diseases increases.
Below in conjunction with test, further illustrate fermented soybean dregs feed of the present invention aspect cultivation, the poultry of feeding, domestic animal and strengthening the application aspect disease-resistant of poultry, domestic animal.
(1) the big porker test examples of feeding:
Fermenting bean dregs feed, with 30% of finishing pigs diets weight, is made an addition to join in its daily ration and stirred.By 50 growing and fattening pigs, divide A, two groups of B, A group is control group, B group is experimental group, every group each 25, carries out the contrast experiment of growing-finishing pigs speed.The B group ratio by weight 30% is to fermenting bean dregs feed in feed, and experimental period is 30 days.Result of the test is that the average daily gain of B group is organized 52.17%, P < 0.01 higher than A.
(2) feeding dorking test examples:
Fermenting bean dregs feed, with 15% of daily ration of broiler weight, is made an addition to join in its daily ration and stirred.By 200 broiler chicken, divide A, two groups of B, A group is control group, B group is experimental group, every group each 100, carries out the contrast experiment of the speed of growth.The B group ratio by weight 15% is to fermenting bean dregs feed in feed, and experimental period is 20 days.Result of the test is that the average daily gain of B group is organized 35.17%, P < 0.01 higher than A.
The specific embodiment
Below in conjunction with embodiment, the present invention will be further described.
Embodiment 1
(1) first carry out slant strains cultivation: each bacterial classification is used corresponding culture medium and adds 1.5% agar, inoculum concentration 5%, under 25 ℃ of conditions, static cultivation is 20 hours, puts into 4 ℃ of Refrigerator stores.The culture medium of bacterial classification used and the corresponding use of bacterial classification sees the following form 1:
Table 1
| Sequence number | Bacterium name | Numbering | Culture medium |
| 1 | Saccharomycete | AS1.1226 | Peptone 1%, beef extract 0.5%, sodium chloride 0.5%, pH7.2-7.4 |
| 2 | Bacillus coagulans | IFFI10067 | Peptone 1%, beef extract 0.5%, sodium chloride 0.5%, pH7.2-7.4 |
| 3 | Lactobacillus acidophilus | IFFI06005 | Skimmed milk power 10%, pH6.0 |
| 4 | Acetic acid bacteria | AS2.120 | Peptone 2%, yeast extract 1%, glucose 2%, pH5.5 |
(2) liquid one-level shake-flask seed is cultivated, and proceeds in shaking flask, wherein shaking flask condition of culture: 25 ℃ of temperature, and 80 beats/min of rotating speeds, inoculum concentration 1%, cultivates 19h.
(3) proceed in seed fermentation tank, the bacterial classification that liquid two stage fermentation tank culture, microorganism mother liquor are selected is by the proportioning of feedstuff per ton, to form: 400 milliliters, saccharomycete, 100 milliliters of bacillus coagulanses, 300 milliliters of lactobacillus acidophilus, 10 milliliters of acetic acid bacterias.Bacterial classification is combined according to the above ratio, and 10 revs/min of mixing speeds, cultivate 30h, make microorganism mother liquor.
(4) preparation of bean dregs mixture: form by weight proportion per ton: bean dregs 29%, black sesame powder 13%, dandelion powder 14%, sodium chloride 0.3%, carbonic hydroammonium 0.7%, water 43%, make fermenting bean dregs feedstuff soln.
(5) on microorganism mother liquor, inoculate bean dregs mixture, stirring at normal temperature mixes, and packs polybag or Plastic Drum into, vacuumizes, be stacked at indoorly to maintain the temperature at 10 ℃ with top fermentation 15-30 days, and 15 ℃ of fermentation 10-15 days, 20 ℃ with top fermentation 7-10 days.Make fermenting bean dregs feed (finished product).
Finished color is isabelline the fragrant and sesame fragrance of strong acid.PH value 4.5, wherein thick protein brings up to 15, and organic acid content reaches 1.4% and 70% for Pfansteihl, can directly substitute bran feed and be absorbed by livestock.
Embodiment 2
(1) first carry out slant strains cultivation: each bacterial classification is used corresponding culture medium and adds 2% agar, inoculum concentration 8%, under 30 ℃ of conditions, static cultivation is 40 hours, puts into 4 ℃ of Refrigerator stores.The culture medium of bacterial classification used and the corresponding use of bacterial classification sees the following form 1:
Table 1
| Sequence number | Bacterium name | Numbering | Inclined-plane is cultivated |
| 1 | Saccharomycete | AS1.1226 | Peptone 1%, beef extract 0.5%, sodium chloride 0.5%, pH7.4 |
| 2 | Bacillus coagulans | IFFI10067 | Peptone 1%, beef extract 0.5%, sodium chloride 0.5%, pH7.2 |
| 3 | Lactobacillus acidophilus | IFFI06005 | Skimmed milk power 10%, pH6.0 |
| 4 | Acetic acid bacteria | AS2.120 | Peptone 2%, yeast extract 1%, glucose 2%, pH5.5 |
(2) liquid one-level shake-flask seed is cultivated, wherein shaking flask condition of culture: 35 ℃ of temperature, and 160 beats/min of rotating speeds, inoculum concentration 2%, cultivates 25h.
(3) bacterial classification that liquid two stage fermentation tank culture, microorganism mother liquor are selected is by the proportioning of feedstuff per ton, to form: 400 milliliters, saccharomycete, 150 milliliters of bacillus coagulanses, 350 milliliters of lactobacillus acidophilus, 50 milliliters of acetic acid bacterias.Bacterial classification is combined according to the above ratio and makes microorganism mother liquor,
(4) preparation of bean dregs mixture: form by weight proportion per ton: bean dregs 23%, black sesame powder 12.4%, dandelion powder 16%, sodium chloride 0.6%, carbonic hydroammonium 1.0%, water 47%, mix, make fermenting bean dregs feedstuff soln.
(5) on microorganism mother liquor, inoculate bean dregs mixture, through processing, vacuum packaging, makes fermenting bean dregs feed (finished product).
Finished color is isabelline the fragrant and sesame fragrance of strong acid.PH value 4.5, wherein thick protein brings up to 17%, and organic acid content reaches 1.8%, and 80% be Pfansteihl, can directly substitute bran feed and be absorbed by livestock.
Claims (2)
1. bean dregs feed of a microorganism fermentation and preparation method thereof, is characterized in that, it is through fermentation, to make feed by inoculate bean dregs mixture in microorganism mother liquor; The bacterial classification that wherein said microorganism mother liquor is selected, by the proportioning of feedstuff per ton is: saccharomycete 400-450 milliliter, bacillus coagulans 150-250 milliliter, lactobacillus acidophilus 300-400 milliliter, acetic acid bacteria 10-100 milliliter; Wherein said bean dregs mixture by weight proportion per ton is: bean dregs 14-34%, black sesame powder 10.7-18.7%, dandelion powder 10-18%, sodium chloride 0.3-0.6%, carbonic hydroammonium 0.7-1.0%, water 40-52%.
2. bean dregs feed of preparing a kind of microorganism fermentation described in claim 1 and preparation method thereof, it is characterized in that being undertaken by following step: (1) first carries out slant strains cultivation, through the cultivation of liquid one-level shake-flask seed, liquid two stage, cultivate, bacterial classification is combined according to the above ratio and makes microorganism mother liquor, then be inoculated in bean dregs mixture, process by fermentation, make fermenting bean dregs feed; Wherein said slant strains culture medium is: 1) saccharomycete: peptone 1%, beef extract 0.5%, sodium chloride 0.5%, pH7.2-7.4; 2) bacillus coagulans: peptone 1%, beef extract 0.5%, sodium chloride 0.5%, pH7.2-7.4; 3) lactobacillus acidophilus: skimmed milk power 10%, pH6.0; 4) acetic acid bacteria: peptone 2%, yeast extract 1%, glucose 2%, pH5.5; (2) each bacterial classification is used corresponding culture medium and adds 1.5-2.5% agar, inoculum concentration 5-10%, and under 25-35 ℃ of condition, static cultivation 20-48 hour, puts into 4 ℃ of Refrigerator stores; Described shaking flask condition of culture: temperature 25-40 ℃, rotating speed 80-180 beat/min, inoculum concentration 1-3%, cultivates 19-30h; (3) by the proportioning of feedstuff per ton, form: saccharomycete 400-500 milliliter, bacillus coagulans 100-200 milliliter, lactobacillus acidophilus 300-400 milliliter, acetic acid bacteria 10-90 milliliter; (4) preparation of bean dregs mixture: form bean dregs 14-34%, black sesame powder 10.7-18.7%, dandelion powder 10-18%, sodium chloride 0.3-0.6%, carbonic hydroammonium 0.7-1.0%, water 40-52% by weight proportion per ton; (5) microbe inoculation mother liquor on bean dregs mixture, stirring at normal temperature mixes, and packs polybag or Plastic Drum into, vacuumize, be stacked at and indoorly maintain the temperature at 10 ℃ with top fermentation 15-30 days, 15 ℃ of fermentation 10-15 days, 20 ℃ with top fermentation 7-10 days, makes fermenting bean dregs feed.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104026333A (en) * | 2014-05-22 | 2014-09-10 | 王海兴 | Beef flavor chicken feed |
| CN110301526A (en) * | 2019-08-08 | 2019-10-08 | 陕西文岭微生物科技有限公司 | Complex micro organism fungicide and its method for preparing bioactive feed |
| CN110973348A (en) * | 2019-11-11 | 2020-04-10 | 华南农业大学 | Method for fermenting bean dregs by mixed strains |
| CN112790273A (en) * | 2020-12-31 | 2021-05-14 | 安徽丰原发酵技术工程研究有限公司 | Method for recovering L-lactic acid fermentation thallus and continuously producing fermented feed |
| CN115715563A (en) * | 2022-08-29 | 2023-02-28 | 吉林大学 | Fermented bean dregs, preparation method thereof and application thereof in reducing pig raising cost |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104026333A (en) * | 2014-05-22 | 2014-09-10 | 王海兴 | Beef flavor chicken feed |
| CN110301526A (en) * | 2019-08-08 | 2019-10-08 | 陕西文岭微生物科技有限公司 | Complex micro organism fungicide and its method for preparing bioactive feed |
| CN110973348A (en) * | 2019-11-11 | 2020-04-10 | 华南农业大学 | Method for fermenting bean dregs by mixed strains |
| CN112790273A (en) * | 2020-12-31 | 2021-05-14 | 安徽丰原发酵技术工程研究有限公司 | Method for recovering L-lactic acid fermentation thallus and continuously producing fermented feed |
| CN115715563A (en) * | 2022-08-29 | 2023-02-28 | 吉林大学 | Fermented bean dregs, preparation method thereof and application thereof in reducing pig raising cost |
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Application publication date: 20140219 |