CN107712266A - Secondary fermentation grain slag produces the method and application method of high activity high nutrition feed - Google Patents
Secondary fermentation grain slag produces the method and application method of high activity high nutrition feed Download PDFInfo
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Abstract
The invention discloses the method and application method of a kind of secondary fermentation grain slag production high activity high nutrition feed, it is made up of seven kinds of raw materials of the siccatives such as the fodder yeasts such as the edible mushrooms such as flat mushroom, Pleurotus nebrodensis, agrocybe, pleurotus eryngii, delicious lactarius, candida utili, geotrichum candidum, Bacillus, lactic acid bacteria, saccharomyces cerevisiae, waste matter, grass meal, rice bran, wheat bran；The secondary fermentation grain slag production high activity high nutrition feed that the present invention obtains further degrades cellulose and high molecular weight protein in anaerobic fermentation, generates cellulase, organic acid, probiotic, antibacterial peptide etc.；After aerobic and anaerobism two-step fermentation, vegetable protein changes into edible fungi silk-fibroin and candida utili albumen；Contain substantial amounts of biology enzyme, active probiotic, amino acid, edible fungi polysaccharide, edible mushroom polypeptide, probiotic in fermented feed, biology enzyme is favorably improved digestion, edible fungi polysaccharide helps raising immunity, and edible fungi polysaccharide polypeptide and probiotic reduce pathogen and improve speed of growth etc..
Description
Technical field：
The invention belongs to field of microbial fermentation, and in particular to a kind of side of secondary fermentation grain slag production high activity high nutrition feed
Method and application method.
Background technology：
China is a bean product, drinks, soy sauce, vinegar production and consumption big country, bean dregs, schlempe, residue of soya, acid-sludge etc. distillers grains and dregs money
Source very abundant.Current all kinds of poor slags it is main be directly used in animal-breeding or biofermentation after feed.Various poor residue proteins
Content is low, and fiber content is high, it is difficult to be decomposed by the various digestive ferments in animal body, the utilization rate of active components in various poor slags
It is low, cause the waste matter class wasting of resources.At present in the bio-fermentation agent sold on the market due to strain self character（As lactic acid bacteria produces acid
Too high, saccharomyces cerevisiae production alcohol is too high, the biological enzyme activity of Bacillus is low）Or the proportioning between probiotics is improper, fermenting
During poor slag, in addition to forming substantial amounts of active probiotic etc., substantial amounts of organic acid, alcohol etc. are also created, is produced simultaneously
Raw biology enzyme is few, not thorough to the cellulose in waste matter, high molecular weight protein degraded.The feed fermented passes through in feeding animals
Often problems with occur there is problems with.1）Bacillus generation enzyme activity is low（Cellulase activity, protease activity etc.）, in waste matter
Cellulose and high molecular weight protein degraded it is not thorough, cause animal digestion it is bad, diarrhoea the problems such as；2）Lactic acid bacteria production organic acid is high,
Palatability is poor, and 3）Saccharomyces cerevisiae production alcoholic strength height causes the thing of animal alcoholism to occur again and again.
Most of fermentation feedstuff strain is Bacillus（Bacillus subtilis, bacillus licheniformis, bafillus natto）、
Saccharomycete（Candida tropicalis, candida utili and saccharomyces cerevisiae, geotrichum candidum）, lactic acid bacteria.Fermentation is used as using edible mushroom
The report of feed is less, and edible mushroom enzyme activity is high, can be simultaneously more containing edible fungi polysaccharide with decomposition of cellulose and macromolecule protein
Peptide, it can both improve protein content in feed, improve immunity（Reduce disease）, improve the speed of growth（Improve economic effect
Benefit）, the quality of meat can also be improved.
It is most of to use a step anaerobic ferment process at present in the production technology of biological fermentation feed（Typically using lactic acid bacteria,
The strains such as Bacillus, saccharomyces cerevisiae are put into anaerobic fermentation in sealing container after preparing according to a certain percentage）, due to being anaerobism hair
Ferment, caused biology enzyme is fewer during the fermentation, and crude fibre, high molecular weight protein are degraded the problems such as not thorough.
It is more to the report of its preparation technology at present in biological fermentation feed, and to its feeding method and its feeding effect
The report of fruit is less, and this patent does a more comprehensive report of ratio to the application method of fermented feed and the feeding effect of fermented feed
Road.
The content of the invention：
It is an object of the invention to provide a kind of secondary fermentation grain slag production high activity high nutrition feed method and application method,
Such a feed greatly degrades the crude fibre and ANFs of waste matter class, contains abundant probiotics, polysaccharide polypeptide, biology
Enzyme, amino acid, small peptide, containing a certain amount of organic acid, alcohol, letting animals feed diarrhoea can be greatly lowered, improve to raise and move
The thing speed of growth, improve food conversion ratio, improve meat etc., poor slag is fully utilized as feed.
The technical problems to be solved by the invention are achieved through the following technical solutions：Secondary fermentation grain slag produces high activity
The method and application method of high nutrition feed, it is by the edible mushrooms such as flat mushroom, Pleurotus nebrodensis, agrocybe, pleurotus eryngii, delicious lactarius 1, production protein
The fodder yeasts such as Candida, geotrichum candidum 2, Bacillus 3, lactic acid bacteria 4, saccharomyces cerevisiae 5, waste matter 6, grass meal, rice bran, wheat bran etc. are dry
Expect that 7 seven kinds of raw materials pass through the preparation 8 of strain, the mixing 9 of raw material, aerobic fermentation 10, anaerobic fermentation 11, dry 12 5 technique streams
Journey completes its production method, and the proportioning of its raw material is：The ︰ of the edible mushrooms such as flat mushroom, Pleurotus nebrodensis, agrocybe, pleurotus eryngii, delicious lactarius 1
The ︰ grass meals of 5 ︰ waste matters of the fodder yeasts such as candida utili, geotrichum candidum 2 ︰ Bacillus, 3 ︰ lactic acid bacterias, 4 ︰ saccharomyces cerevisiaes 6, rice bran, wheat bran
Deng the ︰ 10~20 of 0~1 ︰ of siccative 7=10~15 ︰, 1~5 ︰, 1~2 ︰, 0~1 ︰ 60~70；
The preparation 8 of strain：（1）The preparation of liquid spawn
One piece of 1 pickings of edible mushroom such as the flat mushroom activated by flat board, Pleurotus nebrodensis, agrocybe, pleurotus eryngii, delicious lactarius are linked into
In seed culture medium, its condition of culture is：PDA culture medium, 26 DEG C -28 DEG C, shaking speed 150-180r/min, cultivate 48h；
The rings of 2 picking of fodder yeast one such as the candida utili by flat board activation, geotrichum candidum are linked into seed culture medium,
Its condition of culture is：PDA culture medium, 26 DEG C -28 DEG C, shaking speed 150-180r/min, cultivate 24h；
The ring of 3 picking of Bacillus one activated by flat board is linked into seed culture medium, its condition of culture is：Nutrient broth
Culture medium, 35 DEG C -37 DEG C, shaking speed 150-180r/min, cultivate 24h；
The ring of 4 picking of lactic acid bacteria one by flat board activation is linked into seed culture medium, its condition of culture is：MRS culture mediums,
35 DEG C -37 DEG C, natural pH, with quiescent culture 24h after sealed membrane good seal seed culture bottle bottleneck；
The ring of 5 picking of saccharomyces cerevisiae one by flat board activation is linked into seed culture medium, its condition of culture is：PDA is cultivated
Base, 35 DEG C -37 DEG C, natural pH, shaking speed 150-180r/min, cultivate 24h；
（2）The preparation of solid spawn
The liquid seeds of the edible mushrooms such as the flat mushroom prepared, Pleurotus nebrodensis, agrocybe, pleurotus eryngii, delicious lactarius 1 are inoculated with by 10%-15%
In amount access solid medium, solid medium is：30% corn flour, 40% wheat bran, 20% rice bran, 10% dregs of beans, 0.1%MgSO4、
0.1%KH2PO4, quiescent culture time 48h, 26 DEG C -28 DEG C of cultivation temperature；
The liquid seeds of the fodder yeasts such as the candida utili prepared, geotrichum candidum 2 are linked into solid medium by 1%-5%
In；
The liquid seeds of Bacillus 3 prepared are linked into solid fermentation culture medium by 1%-5%, solid medium is：50% bran
Skin, 37.5% dregs of beans, 12.5% corn flour, 1% glucose, 1% ammonium sulfate；Incubation time 24h, 37 DEG C of cultivation temperature；
The liquid seeds of saccharomyces cerevisiae 5 prepared are linked into solid medium by 1%-5%, wheat bran is base-material, 1.5% grape
Sugar, K2HPO40.1%, incubation time 12-24h, 32-37 DEG C of cultivation temperature；
The seed liquor of lactic acid bacteria 4 prepared is linked into solid medium by 1%-5%, solid medium is：65% corn flour,
20% dregs of beans, 15% rice bran,
The mixing 9 of raw material：The siccatives 7 such as grass meal, rice bran, wheat bran are added in fresh waste matter 6 and are mixed, its water content is existed
50%-70%；
Aerobic fermentation 10：Flat mushroom, Bai Ling are added in the raw material mixed to the siccative 7 such as above-mentioned waste matter 6 and grass meal, rice bran, wheat bran
The solid fermentation seed of the fodder yeast such as the edible mushrooms such as mushroom, agrocybe, pleurotus eryngii, delicious lactarius 1 and candida utili, geotrichum candidum 2,
Edible fungi inoculation amount 10%-15%, fodder yeast inoculum concentration 1%-5%, standing for fermentation 48h, 25 DEG C -28 DEG C of fermentation temperature, proferment
Expect thickness 20cm-40cm；
Anaerobic fermentation 11：The good raw material of aerobic fermentation in upper step is added into probiotics solid seed, its ratio is Bacillus：Ferment
Female bacterium：Lactic acid bacteria=1:0.8:0.8, it is put into anaerobic fermentation pond or anaerobic fermentation tower and ferments after stirring and evenly mixing, fermentation time is
24-48h, 32-37 DEG C of temperature；
Dry 12：Processing is dried in upper step fermentation material, makes its water content between 20%-30%.
As a result of above technical scheme, its goal of the invention is preferably realized, the secondary fermentation grain that the present invention obtains
Make use of the moisture of waste matter class in slag production high activity high nutrition feed biological fermentation process, avoid the drying of waste matter class into
This；Edible mushroom being combined with candida utili is employed during aerobic fermentation, edible mushroom cellulase activity is high, fully divides
The cellulose for having solved waste matter class forms small-molecule substance, and produces edible fungi polysaccharide polypeptide, and candida utili makes full use of
The small-molecule substance that edible mushroom decomposition of cellulose is formed, form mycoprotein.Therefore fully decomposed during aerobic fermentation
Cellulose, generate cellulase, hemicellulase, protease, edible mushroom polypeptide, edible fungi polysaccharide, mycoprotein（Including food
With bacterium mycelian protein, candida utili albumen）；Probiotic combinations are used in anaerobic fermentation process（Bacillus, saccharomyces cerevisiae,
Lactic acid bacteria）, Bacillus makes full use of oxygen and produces biology enzyme during the fermentation（Protease, cellulase etc.）, further drop
The cellulose and macro-molecular protein not decomposed completely in solution aerobic fermentation form small-molecule substance, are lactic acid bacteria and saccharomycete
Growth provides anaerobic environment and nutriment, lactic acid bacteria and saccharomycete produce organic acid in fermentation（Lactic acid, acetic acid, propionic acid etc.）
With alcohol and other metabolites.Therefore, cellulose and high molecular weight protein are further degraded in anaerobic fermentation, generates fibre
Tie up plain enzyme, protease, organic acid, alcohol, probiotic, antibacterial peptide, amino acid, small peptide, vitamin etc.；Pass through aerobic and anaerobism two
For pH in step fermentation after fermentation feed between 4.5-5.5, alcoholic strength is moderate, solves caused by organic acid content height in fermentation
Palatability difference the problem of and alcoholic strength height caused by alcoholism problem；After aerobic and anaerobism two-step fermentation, plant egg
Edible fungi silk-fibroin and candida utili albumen are changed into vain, protein content is greatly improved；By aerobic with detesting
Oxygen two-step fermentation after fermentation feed nutrient is high, and cellulose and high molecular weight protein are degraded to greatest extent, fermented feed
In containing substantial amounts of biology enzyme, active probiotic, amino acid, edible fungi polysaccharide, edible mushroom polypeptide, probiotic, biology enzyme helps
Digested in improving, edible fungi polysaccharide helps raising immunity, and edible fungi polysaccharide polypeptide and probiotic reduce pathogen and improve growth
Speed etc..
Brief description of the drawings：
Accompanying drawing 1 is present invention process flow chart.
Description of reference numerals is shown in specification last page form.
Embodiment：
The content of the invention is described further with reference to embodiment：
Embodiment 1：
From the content of the invention, secondary fermentation grain slag produces the method and application method of high activity high nutrition feed, it by flat mushroom,
The fodder yeasts such as the edible mushrooms such as Pleurotus nebrodensis, agrocybe, pleurotus eryngii, delicious lactarius 1, candida utili, geotrichum candidum 2, Bacillus 3,
7 seven kinds of raw materials of the siccatives such as lactic acid bacteria 4, saccharomyces cerevisiae 5, waste matter 6, grass meal, rice bran, wheat bran by the preparation 8 of strain, raw material it is mixed
Its production method is completed in conjunction 9, aerobic fermentation 10, anaerobic fermentation 11, dry 12 5 technological processes, and the proportioning of its raw material is：
The ︰ gemma of fodder yeast 2 such as the ︰ candida utili of the edible mushrooms such as flat mushroom, Pleurotus nebrodensis, agrocybe, pleurotus eryngii, delicious lactarius 1, geotrichum candidum
The ︰ of 0~1 ︰ of siccative 7=10~15 ︰, 1~5 ︰, 1~2 ︰ such as the ︰ grass meals of 5 ︰ waste matters of bacterium 3 ︰ lactic acid bacterias, 4 ︰ saccharomyces cerevisiaes 6, rice bran, wheat bran 0~1
60~70 ︰ 10~20；
The preparation 8 of strain：（1）The preparation of liquid spawn
One piece of 1 pickings of edible mushroom such as the flat mushroom activated by flat board, Pleurotus nebrodensis, agrocybe, pleurotus eryngii, delicious lactarius are linked into
In seed culture medium, its condition of culture is：PDA culture medium, 26 DEG C -28 DEG C, shaking speed 150-180r/min, cultivate 48h；
The rings of 2 picking of fodder yeast one such as the candida utili by flat board activation, geotrichum candidum are linked into seed culture medium,
Its condition of culture is：PDA culture medium, 26 DEG C -28 DEG C, shaking speed 150-180r/min, cultivate 24h；
The ring of 3 picking of Bacillus one activated by flat board is linked into seed culture medium, its condition of culture is：Nutrient broth
Culture medium, 35 DEG C -37 DEG C, shaking speed 150-180r/min, cultivate 24h；
The ring of 4 picking of lactic acid bacteria one by flat board activation is linked into seed culture medium, its condition of culture is：MRS culture mediums,
35 DEG C -37 DEG C, natural pH, with quiescent culture 24h after sealed membrane good seal seed culture bottle bottleneck；
The ring of 5 picking of saccharomyces cerevisiae one by flat board activation is linked into seed culture medium, its condition of culture is：PDA is cultivated
Base, 35 DEG C -37 DEG C, natural pH, shaking speed 150-180r/min, cultivate 24h；
（2）The preparation of solid spawn
The liquid seeds of the edible mushrooms such as the flat mushroom prepared, Pleurotus nebrodensis, agrocybe, pleurotus eryngii, delicious lactarius 1 are inoculated with by 10%-15%
In amount access solid medium, solid medium is：30% corn flour, 40% wheat bran, 20% rice bran, 10% dregs of beans, 0.1%MgSO4、
0.1%KH2PO4, quiescent culture time 48h, 26 DEG C -28 DEG C of cultivation temperature；
The liquid seeds of the fodder yeasts such as the candida utili prepared, geotrichum candidum 2 are linked into solid medium by 1%-5%
In；
The liquid seeds of Bacillus 3 prepared are linked into solid fermentation culture medium by 1%-5%, solid medium is：50% bran
Skin, 37.5% dregs of beans, 12.5% corn flour, 1% glucose, 1% ammonium sulfate；Incubation time 24h, 37 DEG C of cultivation temperature；
The liquid seeds of saccharomyces cerevisiae 5 prepared are linked into solid medium by 1%-5%, wheat bran is base-material, 1.5% grape
Sugar, K2HPO40.1%, incubation time 12-24h, 32-37 DEG C of cultivation temperature；
The seed liquor of lactic acid bacteria 4 prepared is linked into solid medium by 1%-5%, solid medium is：65% corn flour,
20% dregs of beans, 15% rice bran,
The mixing 9 of raw material：The siccatives 7 such as grass meal, rice bran, wheat bran are added in fresh waste matter 6 and are mixed, its water content is existed
50%-70%；
Aerobic fermentation 10：Flat mushroom, Bai Ling are added in the raw material mixed to the siccative 7 such as above-mentioned waste matter 6 and grass meal, rice bran, wheat bran
The solid fermentation seed of the fodder yeast such as the edible mushrooms such as mushroom, agrocybe, pleurotus eryngii, delicious lactarius 1 and candida utili, geotrichum candidum 2,
Edible fungi inoculation amount 10%-15%, fodder yeast inoculum concentration 1%-5%, standing for fermentation 48h, 25 DEG C -28 DEG C of fermentation temperature, proferment
Expect thickness 20cm-40cm；
Anaerobic fermentation 11：The good raw material of aerobic fermentation in upper step is added into probiotics solid seed, its ratio is Bacillus：Ferment
Female bacterium：Lactic acid bacteria=1:0.8:0.8, it is put into anaerobic fermentation pond or anaerobic fermentation tower and ferments after stirring and evenly mixing, fermentation time is
24-48h, 32-37 DEG C of temperature；
Dry 12：Processing is dried in upper step fermentation material, makes its water content between 20%-30%.
The edible mushrooms 1 such as flat mushroom of the present invention, Pleurotus nebrodensis, agrocybe, pleurotus eryngii, delicious lactarius refer to：Edible mushroom；It is edible
Bacterium is grown with the mycelium of its white or light color in the place containing abundant organic matter, and protein content is generally fresh mushroom 1.5-
6%th, dry mushroom 15-35%, higher than general vegetables, and its amino acid ratio of components is more comprehensive, contains abundant protein and amino
Acid, its content are several times to tens times of general vegetables and fruit.
The fodder yeasts 2 such as candida utili of the present invention, geotrichum candidum refer to：Saccharomycete as animal and fowl fodder
Body；The composition of yeast is relevant with strain, condition of culture.It is general to contain protein 40~65%, fat 1~8%, carbohydrate 25~40%, ash
Divide 6~9%, wherein about 20 kinds of amino acid.The less lysine of content in cereal, tryptophan are richer in yeast
It is rich；It is about higher than soybean by 30% using ammonia particularly when adding methionine.The caloric value of yeast equivalent to beef, and due to containing
There is abundant B family vitamin, usually as protein and the addition feed of vitamin.For raising pig, ox, chicken, duck, mink fish
Class, can receive build up health, reduce disease, weightening is fast, lays eggs and the good economic effects such as more than give milk.
Bacillus 3 of the present invention refers to：A section for bacterium, can form gemma（Endospore）Bacillus or coccus；
By strengthening digestion and absorption function, the natural pigment using nutrition in feed and raw material is fully absorbed, without adding chemistry
Pigment tonyred, Carophyll red cause the harmful substance and influence animal products natural edible flavor on human body, can match in excellence or beauty domestic
Livestock meat.Animal product degree of staining and edible flavor can naturally be increased, pig skin is ruddy, and hair color is shinny.
Lactic acid bacteria 4 of the present invention refers to：A kind of bacterium that fermentable carbohydrate can be utilized to produce a large amount of lactic acid
Common name；Growth of animal can be promoted, regulation composition of gut flora, microecological balance is maintained, so as to improve gastrointestinal function；Carry
High food digestion rate and biological value；Serum cholesterol is reduced, controls endotoxin；Suppress corrupt bacteria growing in enteron aisle：Raising machine
Body immunity etc..
Saccharomyces cerevisiae 5 of the present invention refers to：Also known as Saccharomyces cerevisiae or budding yeast；It can effectively improve livestock and poultry alimentary tract
Colony balance, enhancing immunity of organisms, abundant nutriment is provided, so as to reach preventing and treating disease of digestive tract and promote growth etc.
Multiple action, have the characteristics that it is nontoxic, have no side effect, be pollution-free, not developing immunity to drugs, be a kind of green feed addictive.
Waste matter 6 of the present invention refers to：Brew alcoholic beverages remaining dregs；For a kind of raw material of the present invention.
The siccatives 7 such as grass meal of the present invention, rice bran, wheat bran refer to：Feedstuff；The appropriate collocation rice bran in pig diet
The dregs of rice can reduce feeding cost, and its feeding value is equivalent to corn；Wheat bran is the outermost epidermis of wheat, is contained in wheat bran more
Protein, nutritive value is higher, and biological value is at a relatively high, can be matched in excellence or beauty with Chicken Albumin.
| Reference numerals | Mark title | Reference numerals | Mark title |
| 1 | The edible mushrooms such as flat mushroom, Pleurotus nebrodensis, agrocybe, pleurotus eryngii, delicious lactarius 1 | 7 | The siccatives such as grass meal, rice bran, wheat bran 7 |
| 2 | The fodder yeasts such as candida utili, geotrichum candidum 2 | 8 | The preparation 8 of strain |
| 3 | Bacillus 3 | 9 | The mixing 9 of raw material |
| 4 | Lactic acid bacteria 4 | 10 | Aerobic fermentation 10 |
| 5 | Saccharomyces cerevisiae 5 | 11 | Anaerobic fermentation 11 |
| 6 | Waste matter 6 | 12 | Dry 12 |
Claims (1)
1. secondary fermentation grain slag produces the method and application method of high activity high nutrition feed, it is characterized in that it is by flat mushroom, Bai Ling
The fodder yeast such as the edible mushrooms such as mushroom, agrocybe, pleurotus eryngii, delicious lactarius (1), candida utili, geotrichum candidum (2), Bacillus
(3), the system that (7) seven kinds of raw materials of the siccative such as lactic acid bacteria (4), saccharomyces cerevisiae (5), waste matter (6), grass meal, rice bran, wheat bran pass through strain
Its production is completed in standby (8), the mixing (9) of raw material, aerobic fermentation (10), anaerobic fermentation (11), dry (12) five technological processes
Method, the proportioning of its raw material are：The edible mushrooms such as flat mushroom, Pleurotus nebrodensis, agrocybe, pleurotus eryngii, delicious lactarius (1) ︰ Candida utilis ferment
The fodder yeasts such as mother, geotrichum candidum (2) ︰ Bacillus (3) ︰ lactic acid bacterias (4) ︰ saccharomyces cerevisiaes (5) ︰ waste matters (6) ︰ grass meals, rice bran, wheat bran
Deng the ︰ 10~20 of 0~1 ︰ of siccative (7)=10~15 ︰ 1~5 ︰, 1~2 ︰, 0~1 ︰ 60~70；
The preparation (8) of strain：（1）The preparation of liquid spawn
By the one piece of access of edible mushroom (1) pickings such as the flat mushroom activated by flat board, Pleurotus nebrodensis, agrocybe, pleurotus eryngii, delicious lactarius
Into seed culture medium, its condition of culture is：PDA culture medium, 26 DEG C -28 DEG C, shaking speed 150-180r/min, cultivate 48h；
The rings of fodder yeast (2) picking one such as the candida utili by flat board activation, geotrichum candidum are linked into seed culture medium
In, its condition of culture is：PDA culture medium, 26 DEG C -28 DEG C, shaking speed 150-180r/min, cultivate 24h；
The ring of Bacillus (3) picking one activated by flat board is linked into seed culture medium, its condition of culture is：Nutrient meat
Soup culture medium, 35 DEG C -37 DEG C, shaking speed 150-180r/min, cultivate 24h；
The ring of lactic acid bacteria (4) picking one by flat board activation is linked into seed culture medium, its condition of culture is：MRS is cultivated
Base, 35 DEG C -37 DEG C, natural pH, with quiescent culture 24h after sealed membrane good seal seed culture bottle bottleneck；
The ring of saccharomyces cerevisiae (5) picking one by flat board activation is linked into seed culture medium, its condition of culture is：PDA is trained
Base is supported, 35 DEG C -37 DEG C, natural pH, shaking speed 150-180r/min, cultivates 24h；
（2）The preparation of solid spawn
The edible mushrooms such as the flat mushroom prepared, Pleurotus nebrodensis, agrocybe, pleurotus eryngii, delicious lactarius (1) liquid seeds are connect by 10%-15%
In kind amount access solid medium, solid medium is：30% corn flour, 40% wheat bran, 20% rice bran, 10% dregs of beans, 0.1%
MgSO4、0.1%KH2PO4, quiescent culture time 48h, 26 DEG C -28 DEG C of cultivation temperature；
The fodder yeasts such as the candida utili prepared, geotrichum candidum (2) liquid seeds are linked into solid medium by 1%-5%
In；
The Bacillus prepared (3) liquid seeds are linked into solid fermentation culture medium by 1%-5%, solid medium is：50%
Wheat bran, 37.5% dregs of beans, 12.5% corn flour, 1% glucose, 1% ammonium sulfate；Incubation time 24h, 37 DEG C of cultivation temperature；
The saccharomyces cerevisiae prepared (5) liquid seeds are linked into solid medium by 1%-5%, wheat bran is base-material, 1.5% Portugal
Grape sugar, K2HPO40.1%, incubation time 12-24h, 32-37 DEG C of cultivation temperature；
The lactic acid bacteria prepared (4) seed liquor is linked into solid medium by 1%-5%, solid medium is：65% corn
Powder, 20% dregs of beans, 15% rice bran,
The mixing (9) of raw material：The siccatives (7) such as grass meal, rice bran, wheat bran are added in fresh waste matter (6) and are mixed, make its aqueous
Amount is in 50%-70%；
Aerobic fermentation (10)：Added in the raw material mixed to the siccative such as above-mentioned waste matter (6) and grass meal, rice bran, wheat bran (7) flat
The fodder yeast such as the edible mushrooms such as mushroom, Pleurotus nebrodensis, agrocybe, pleurotus eryngii, delicious lactarius (1) and candida utili, geotrichum candidum (2) is solid
Body fermentation seed, edible fungi inoculation amount 10%-15%, fodder yeast inoculum concentration 1%-5%, standing for fermentation 48h, 25 DEG C of fermentation temperature-
28 DEG C, fermentation raw material thickness 20cm-40cm；
Anaerobic fermentation (11)：The good raw material of aerobic fermentation in upper step is added into probiotics solid seed, its ratio is Bacillus：
Saccharomycete：Lactic acid bacteria=1:0.8:0.8, it is put into anaerobic fermentation pond or anaerobic fermentation tower and ferments after stirring and evenly mixing, fermentation time
For 24-48h, 32-37 DEG C of temperature；
Dry (12)：Processing is dried in upper step fermentation material, makes its water content between 20%-30%.
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| CN108703267A (en) * | 2018-04-20 | 2018-10-26 | 广东壹号地方猪研究院有限公司 | Application of the fermentation pomace in terms of improving meat quality and Development of Digestive Organs |
| CN110037173A (en) * | 2019-05-14 | 2019-07-23 | 史峥 | A kind of pomace and dreg fodder and preparation method thereof |
| CN110074252A (en) * | 2019-05-20 | 2019-08-02 | 浙江康星生物科技有限公司 | A kind of the fermented feed processing method and application of the high efficiency, low cost based on bean dregs |
| CN113892556A (en) * | 2021-09-29 | 2022-01-07 | 四川大学青岛研究院 | Biological protein feed prepared from waste vinasse of Jimo old wine and preparation method thereof |
| CN115868581A (en) * | 2023-01-07 | 2023-03-31 | 山东省农业科学院畜牧兽医研究所 | Beef cattle fermented feed and preparation method thereof |
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| CN113892556A (en) * | 2021-09-29 | 2022-01-07 | 四川大学青岛研究院 | Biological protein feed prepared from waste vinasse of Jimo old wine and preparation method thereof |
| CN115868581A (en) * | 2023-01-07 | 2023-03-31 | 山东省农业科学院畜牧兽医研究所 | Beef cattle fermented feed and preparation method thereof |
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