CN103535511A - Method for producing feed with rich peptide and rich prebiotics by fermenting high-temperature soybean meal - Google Patents

Method for producing feed with rich peptide and rich prebiotics by fermenting high-temperature soybean meal Download PDF

Info

Publication number
CN103535511A
CN103535511A CN201310469753.2A CN201310469753A CN103535511A CN 103535511 A CN103535511 A CN 103535511A CN 201310469753 A CN201310469753 A CN 201310469753A CN 103535511 A CN103535511 A CN 103535511A
Authority
CN
China
Prior art keywords
rich
beans
dregs
temperature
fermentation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CN201310469753.2A
Other languages
Chinese (zh)
Other versions
CN103535511B (en
Inventor
杨瑞金
李舒宇
徐张贤
金妙仁
张燕鹏
郑婷婷
赵伟
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Jiangnan University
Original Assignee
Jiangnan University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Jiangnan University filed Critical Jiangnan University
Priority to CN201310469753.2A priority Critical patent/CN103535511B/en
Publication of CN103535511A publication Critical patent/CN103535511A/en
Application granted granted Critical
Publication of CN103535511B publication Critical patent/CN103535511B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Abstract

The invention discloses a method for producing feed with rich peptide and rich prebiotics by fermenting high-temperature soybean meal, and relates to the technical field of farm products bioprocess. A stain of high-yield protease bacillus subtilis which is sorted from conventional Jiangxi fermented food is used for being matched with brewing yeast and lactobacillus plantarum to carry out the mixed solid fermentation on high-temperature soybean meal, slant activation and liquid enlarge cultivation are carried out, aerobic fermentation is carried out for single inoculation, anaerobic fermentation is carried out for the mixed inoculation, then the high-temperature soybean meal is dried at the temperature of 60 DEG C to obtain a fermented soybean meal feed product in which with protein nutrients are rich, antinutritional factors are passivated almost, the palatability is improved and the bioactivity is high. The method has the advantages that the constitution of the protein and the antinutritional factors of the high-temperature soybean meal fermented product is further improved, the palatability of the fermented product is improved, and the fermented product has the characteristics of rich nutrients, low resistance and palatability.

Description

A kind of high temperature dregs of beans that ferments produces the preparation method of the rich prebiotics feed of rich peptide
Technical field
What the present invention relates to is agricultural product biological processing technical field, is specifically related to the preparation method that a kind of high temperature dregs of beans that ferments produces the rich prebiotics feed of rich peptide.
Background technology
Current feeding dregs of beans is mainly high temperature dregs of beans, high temperature dregs of beans is due to its process high temperature carrying out precipitation treatment in process, and protein denaturation degree is wherein larger, and dissolubility is low, also contain some ANFs, animal is lower to the digestibility of nutriment wherein simultaneously.Fermented bean dregs is a kind of green, efficient dregs of beans treatment technology.Many improvement to fermented bean dregs technique in recent years, the Chinese patent that is 200810219198.7 as application number adopts 3 bacillus subtilis, saccharomyces cerevisiae and lactic acid bacteria are fermented to dregs of beans, the protein in dregs of beans of having degraded to a certain extent, promoted its nutritive value, but the amplitude that gross protein value promotes is not high.Liu Xiaoyan etc. adopt bacillus subtilis, aspergillus oryzae and saccharomyces cerevisiae mixed culture fermentation high temperature dregs of beans, make polypeptide yield in final tunning reach 54.89%, in tunning, content of peptides is 21.47% (butt), than raw material, 4.1 times have been increased, there is preferably nutritive value, but this method inoculum concentration is 25%, fermentation raw material need add wheat bran and molasses to adjust the carbon-nitrogen ratio of culture medium, cost is larger, though the local flavor of fermented bean dregs product is improved, but do not there is sour Flavor, feed before raising and still need acidification, palatability still has the space (Liu Xiaoyan of lifting, Yang Guoli, state-run east. mixed culture solid state fermentation method is produced the research [J] of soya-bean polypeptides feed. feed industry, 2012.33 (6): 51-56).Generally speaking, still there is the problems such as cost is higher, product nutritive value is not high, palatability is lower in high temperature fermentation process of bean dregs at present.
Summary of the invention
For the deficiency existing in prior art, the present invention seeks to be to provide a kind of high temperature dregs of beans that ferments to produce the preparation method of the rich prebiotics feed of rich peptide, have with low cost, product is nutritious, the feature that palatability is good.Can further promote the efficient utilization of protein resource in high temperature dregs of beans.
To achieve these goals, the present invention realizes by the following technical solutions: a kind of high temperature dregs of beans that ferments produces the preparation method of the rich prebiotics feed of rich peptide, its preparation process is: (1) gets respectively a ring on beef extract-peptone slant medium, YPD slant medium and MRS slant medium from original strain bacillus subtilis, saccharomyces cerevisiae and Lactobacillus plantarum, at 30 ℃-37 ℃, cultivate 18h-24h, until grow obvious bacterium colony on inclined-plane, complete actication of culture;
(2) picking one ring in bacterial strain bacillus subtilis and saccharomyces cerevisiae from activating, be inoculated into respectively in beef extract-peptone fluid nutrient medium and YPD fluid nutrient medium, at 30 ℃ of-37 ℃ of temperature, shaking table is cultivated 18h-48h, until the concentration of microorganism reaches 106CFU/mL-108CFU/mL in nutrient solution, stop cultivating, seed liquor is successfully prepared.From activating, picking one ring in bacterial strain Lactobacillus plantarum, is inoculated in MRS fluid nutrient medium, and at 30 ℃ of-37 ℃ of temperature, standing cultivation 24h-48h, makes cell concentration in seed liquor reach 107CFU/mL-108CFU/mL, makes seed liquor;
(3) dregs of beans is carried out to batch mixing, by material-water ratio 1: 0.6-1: 1.2 carry out batch mixing, the dregs of beans pH that initially ferments is 6.0-7.0, then with bacillus subtilis seed liquor in inoculum concentration (by dry Soybean Meal before mixing with water) the access step (2) of 3%-6%, after mixing, aerobic fermentation 24h-48h.After aerobic fermentation, the seed liquor with saccharomyces cerevisiae and Lactobacillus plantarum in the inoculum concentration access step (2) of 1%-3%, seals anaerobic fermentation 48-72h;
(4) after fermentation, sample dry, pulverize at 60 ℃, finally dregs of beans before and after fermentation is carried out to Analysis of Nutritive Composition.
In such scheme, bacillus subtilis slant medium described in described step (1) contains the sodium chloride of 0.3%-0.5% by weight, the peptone of 0.5%-1.0%, the beef extract of 0.1%-0.3% and the agar powder of 1.8%-2.0%, YPD slant medium contains the peptone of 0.8%-1.0% by weight, the yeast extract of 0.3%-0.5%, the glucose of 1.5%-2.0% and the agar powder of 1.8%-2.0%, MRS slant medium contains the peptone of 0.8%-1.0% by weight, the beef extract of 0.5%-0.8%, the yeast extract of 0.1%-0.4%, the glucose of 1.5%-2.0%, the dipotassium hydrogen phosphate of 0.1%-0.2%, the bitter salt of 0.03%-0.06%, the Manganous sulfate monohydrate of 0.01%-0.03% and the agar powder of 1.8%-2.0%, the Tween 80 that simultaneously contains 0.05%-0.1% by volume.Liquid activation medium described in described step (2) is as do not add the culture medium of agar powder on the basis of slant medium.
The present invention adopts many bacterial strains mixed solid fermentation high temperature dregs of beans, make the nutritive peculiarity of dregs of beans obtain remarkable improvement, gross protein value has improved 25.7%, protein degradation is comparatively obvious, nitrogen soluble index has improved 4.02 times, and sour molten protein content has improved 4.89 times, and VBN has reduced by 57.2% compared with the single strain fermentation of bacillus subtilis, the relative raw material dregs of beans of trypsin inhibitor vigor has reduced by 95.6%, also contains 3.86% organic acid and 3.25 * 10 simultaneously 8the active probiotic of CFU/g.Product of the present invention proves through livestock and poultry young baby's culture experiment, this product can fine replacement dregs of beans or part replace fish meal, improve growth of animal performance, promote the utilization rate of feed, there is significant economic benefit.
The specific embodiment
For technological means, creation characteristic that the present invention is realized, reach object and effect is easy to understand, below in conjunction with the specific embodiment, further set forth the present invention.
Embodiment 1: the embodiment of this specific embodiment by the following technical solutions: a kind of high temperature dregs of beans that ferments produces the preparation method of the rich prebiotics feed of rich peptide, its preparation process is: (1) gets respectively a ring on beef extract-peptone slant medium, YPD slant medium and MRS slant medium from original strain bacillus subtilis, saccharomyces cerevisiae and Lactobacillus plantarum, at 30 ℃-37 ℃, cultivate 18h-24h, until grow obvious bacterium colony on inclined-plane, complete actication of culture;
(2) picking one ring in bacterial strain bacillus subtilis and saccharomyces cerevisiae from activating, be inoculated into respectively in beef extract-peptone fluid nutrient medium and YPD fluid nutrient medium, at 30 ℃ of-37 ℃ of temperature, shaking table is cultivated 18h-48h, until the concentration of microorganism reaches 106CFU/mL-108CFU/mL in nutrient solution, stop cultivating, seed liquor is successfully prepared.From activating, picking one ring in bacterial strain Lactobacillus plantarum, is inoculated in MRS fluid nutrient medium, and at 30 ℃ of-37 ℃ of temperature, standing cultivation 24h-48h, makes cell concentration in seed liquor reach 107CFU/mL-108CFU/mL, makes seed liquor;
(3) dregs of beans is carried out to batch mixing, by material-water ratio 1: 0.6-1: 1.2 carry out batch mixing, the dregs of beans pH that initially ferments is 6.0-7.0, then with bacillus subtilis seed liquor in inoculum concentration (by dry Soybean Meal before mixing with water) the access step (2) of 3%-6%, after mixing, aerobic fermentation 24h-48h.After aerobic fermentation, the seed liquor with saccharomyces cerevisiae and Lactobacillus plantarum in the inoculum concentration access step (2) of 1%-3%, seals anaerobic fermentation 48-72h;
(4) after fermentation, sample dry, pulverize at 60 ℃, finally dregs of beans before and after fermentation is carried out to Analysis of Nutritive Composition; Be analyzed as follows:
Testing result 1
Remarks: above content of material all calculates with dry.
Embodiment 2:
Difference from Example 1 is, aerobic fermentation 24h, and sealing anaerobic fermentation 72h, the inoculum concentration of bacillus subtilis seed liquor is 5.4%, the seed liquor inoculum concentration of saccharomyces cerevisiae and Lactobacillus plantarum is 1.8%.Dregs of beans before and after fermentation is carried out to Analysis of Nutritive Composition as follows:
Testing result 2
Remarks: above content of material all calculates with dry.
Embodiment 3:
Difference from Example 2 is, the initial pH of dregs of beans is 6.5, and the total amount that connects of bacillus subtilis seed liquor is 6%, and the inoculum concentration of saccharomyces cerevisiae and Lactobacillus plantarum seed liquor is 3%.Dregs of beans before and after fermentation is carried out to Analysis of Nutritive Composition as follows:
Testing result 3
Remarks: above content of material all calculates with dry.
More than show and described basic principle of the present invention and principal character and advantage of the present invention.The technical staff of the industry should understand; the present invention is not restricted to the described embodiments; that in above-described embodiment and description, describes just illustrates principle of the present invention; without departing from the spirit and scope of the present invention; the present invention also has various changes and modifications, and these changes and improvements all fall in the claimed scope of the invention.The claimed scope of the present invention is defined by appending claims and equivalent thereof.

Claims (5)

1. the high temperature dregs of beans that ferments produces the preparation method of the rich prebiotics feed of rich peptide, it is characterized in that, its preparation process is: (1) gets respectively a ring on beef extract-peptone slant medium, YPD slant medium and MRS slant medium from original strain bacillus subtilis, saccharomyces cerevisiae and Lactobacillus plantarum, at 30 ℃-37 ℃, cultivate 18h-24h, until grow obvious bacterium colony on inclined-plane, complete actication of culture;
(2) picking one ring in bacterial strain bacillus subtilis and saccharomyces cerevisiae from activating, be inoculated into respectively in beef extract-peptone fluid nutrient medium and YPD fluid nutrient medium, at 30 ℃ of-37 ℃ of temperature, shaking table is cultivated 18h-48h, until the concentration of microorganism reaches 106CFU/mL-108CFU/mL in nutrient solution, stop cultivating, seed liquor is successfully prepared.From activating, picking one ring in bacterial strain Lactobacillus plantarum, is inoculated in MRS fluid nutrient medium, and at 30 ℃ of-37 ℃ of temperature, standing cultivation 24h-48h, makes cell concentration in seed liquor reach 107CFU/mL-108CFU/mL, makes seed liquor;
(3) dregs of beans is carried out to batch mixing, by material-water ratio 1: 0.6-1: 1.2 carry out batch mixing, the dregs of beans pH that initially ferments is 6.0-7.0, then with bacillus subtilis seed liquor in inoculum concentration (by dry Soybean Meal before mixing with water) the access step (2) of 3%-6%, after mixing, aerobic fermentation 24h-48h.After aerobic fermentation, the seed liquor with saccharomyces cerevisiae and Lactobacillus plantarum in the inoculum concentration access step (2) of 1%-3%, seals anaerobic fermentation 48-72h;
(4) after fermentation, sample dry, pulverize at 60 ℃, finally dregs of beans before and after fermentation is carried out to Analysis of Nutritive Composition.
2. a kind of high temperature dregs of beans that ferments according to claim 1 produces the preparation method of the rich prebiotics feed of rich peptide, it is characterized in that, bacillus subtilis slant medium described in described step (1) contains the sodium chloride of 0.3%-0.5% by weight, the peptone of 0.5%-1.0%, the beef extract of 0.1%-0.3% and the agar powder of 1.8%-2.0%, YPD slant medium contains the peptone of 0.8%-1.0% by weight, the yeast extract of 0.3%-0.5%, the glucose of 1.5%-2.0% and the agar powder of 1.8%-2.0%, MRS slant medium contains the peptone of 0.8%-1.0% by weight, the beef extract of 0.5%-0.8%, the yeast extract of 0.1%-0.4%, the glucose of 1.5%-2.0%, the dipotassium hydrogen phosphate of 0.1%-0.2%, the bitter salt of 0.03%-0.06%, the Manganous sulfate monohydrate of 0.01%-0.03% and the agar powder of 1.8%-2.0%, the Tween 80 that simultaneously contains 0.05%-0.1% by volume.
3. a kind of high temperature dregs of beans that ferments according to claim 1 produces the preparation method of the rich prebiotics feed of rich peptide, it is characterized in that, the liquid activation medium described in described step (2) is as do not add the culture medium of agar powder on the basis of slant medium.
4. according to a kind of high temperature dregs of beans that ferments described in claim 1,2,3, produce the preparation method of the rich prebiotics feed of rich peptide, it is characterized in that, the dregs of beans after fermentation dried can be used as the feed of pig, chicken and fish etc.
5. a kind of high temperature dregs of beans that ferments according to claim 1 produces the preparation method of the rich prebiotics feed of rich peptide, it is characterized in that, described bacterial classification is bacillus subtilis (Bacil1us Subtilis) 1 strain, saccharomyces cerevisiae (Saccharomyces cerevisiae) 1 strain and Lactobacillus plantarum (Lactobacillus plantarum) 1 strain.
CN201310469753.2A 2013-09-25 2013-09-25 A kind of high temperature dregs of beans that ferments produces the preparation method of the rich prebiotics feed of rich peptide Active CN103535511B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201310469753.2A CN103535511B (en) 2013-09-25 2013-09-25 A kind of high temperature dregs of beans that ferments produces the preparation method of the rich prebiotics feed of rich peptide

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201310469753.2A CN103535511B (en) 2013-09-25 2013-09-25 A kind of high temperature dregs of beans that ferments produces the preparation method of the rich prebiotics feed of rich peptide

Publications (2)

Publication Number Publication Date
CN103535511A true CN103535511A (en) 2014-01-29
CN103535511B CN103535511B (en) 2016-05-18

Family

ID=49959674

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201310469753.2A Active CN103535511B (en) 2013-09-25 2013-09-25 A kind of high temperature dregs of beans that ferments produces the preparation method of the rich prebiotics feed of rich peptide

Country Status (1)

Country Link
CN (1) CN103535511B (en)

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103829040A (en) * 2014-03-18 2014-06-04 广东轻工职业技术学院 Method for compounding and fermenting soybean meal by Chenxiang tea and bacillus subtilis and application
CN104012755A (en) * 2014-06-12 2014-09-03 江南大学 Two-step fermentation method for efficient production of fermented soybean meal
CN104664080A (en) * 2015-02-16 2015-06-03 青岛九和宜生生物科技有限公司 Lactobacillus plantarum fermented soybean meal and production method thereof as well as method for preparing compound feed for scophthalmus maximus
CN105795109A (en) * 2016-03-10 2016-07-27 东北农业大学 Solid-state fermentation method of high-temperature soybean meal by mixed bacterial strains
CN107348097A (en) * 2017-07-31 2017-11-17 浦江县协盈动物饲料技术开发有限公司 Penaeus Vannmei children phase feed
CN108651762A (en) * 2018-04-03 2018-10-16 中国水产科学研究院珠江水产研究所 It is a kind of to improve processing method and broad bean fermentate of the fish to broad bean digestive utilization ratio
CN109156608A (en) * 2018-09-18 2019-01-08 青岛今墨堂生物技术有限公司 A kind of experimental animal special feed and preparation method thereof
CN109329564A (en) * 2018-11-24 2019-02-15 管振平 One breeding hen feed preparation bean pulp fermentation mixed method

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108378223A (en) * 2018-03-19 2018-08-10 安徽希普生物科技有限公司 A kind of preparation method of the feeding fermented dregs of beans of low antigen

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2243678C1 (en) * 2003-07-02 2005-01-10 Общество с ограниченной ответственностью "Биотех-Инжиниринг" Method for preparing protein-vitamin fodder
CN1663422A (en) * 2005-04-19 2005-09-07 单德章 Zymophyte of plant straw feedstuff and its preparation
CN102488089A (en) * 2011-11-18 2012-06-13 吉林省农业科学院 Preparation method of microecological culture feed additive

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2243678C1 (en) * 2003-07-02 2005-01-10 Общество с ограниченной ответственностью "Биотех-Инжиниринг" Method for preparing protein-vitamin fodder
CN1663422A (en) * 2005-04-19 2005-09-07 单德章 Zymophyte of plant straw feedstuff and its preparation
CN102488089A (en) * 2011-11-18 2012-06-13 吉林省农业科学院 Preparation method of microecological culture feed additive

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
朱平军: "多菌种固态生料仿生发酵豆粕的研究", 《中国优秀硕士学位论文全文数据库》 *
胡永娜,等: "微生物固态发酵菜籽粕营养特性的研究", 《中国粮油学报》 *
陈炳钿,等: "复合微生物固态发酵豆粕的工艺条件研究", 《福建畜牧兽医》 *

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103829040A (en) * 2014-03-18 2014-06-04 广东轻工职业技术学院 Method for compounding and fermenting soybean meal by Chenxiang tea and bacillus subtilis and application
CN103829040B (en) * 2014-03-18 2015-10-07 广东轻工职业技术学院 A kind of with the method for Chen Xiangcha and bacillus subtilis composite fermentation dregs of beans and application
CN104012755A (en) * 2014-06-12 2014-09-03 江南大学 Two-step fermentation method for efficient production of fermented soybean meal
CN104664080A (en) * 2015-02-16 2015-06-03 青岛九和宜生生物科技有限公司 Lactobacillus plantarum fermented soybean meal and production method thereof as well as method for preparing compound feed for scophthalmus maximus
CN105795109A (en) * 2016-03-10 2016-07-27 东北农业大学 Solid-state fermentation method of high-temperature soybean meal by mixed bacterial strains
CN107348097A (en) * 2017-07-31 2017-11-17 浦江县协盈动物饲料技术开发有限公司 Penaeus Vannmei children phase feed
CN108651762A (en) * 2018-04-03 2018-10-16 中国水产科学研究院珠江水产研究所 It is a kind of to improve processing method and broad bean fermentate of the fish to broad bean digestive utilization ratio
CN109156608A (en) * 2018-09-18 2019-01-08 青岛今墨堂生物技术有限公司 A kind of experimental animal special feed and preparation method thereof
CN109329564A (en) * 2018-11-24 2019-02-15 管振平 One breeding hen feed preparation bean pulp fermentation mixed method

Also Published As

Publication number Publication date
CN103535511B (en) 2016-05-18

Similar Documents

Publication Publication Date Title
CN103535511B (en) A kind of high temperature dregs of beans that ferments produces the preparation method of the rich prebiotics feed of rich peptide
CN104996722B (en) A kind of method of the step combined ferment feed of multi-cultur es two
CN102823726B (en) It is a kind of improve cottonseed meal content and can detoxification biofermentation method
CN102934736B (en) Method for preparing sweet potato skin/ sweet potato powder dreg fermented feed
CN104472879A (en) Tea residue feed additive and preparation method thereof
CN104824337A (en) Preparation method of fermented soybean meal for feeding
CN103911323A (en) Bacillus licheniformis, bacillus subtilis and lactobacillus plantarum preparation and preparation
CN104054902A (en) Process for producing fermented soybean meal through solid state fermentation of mixed culture
CN104757267A (en) Apple pomace microbial culture starter and method for producing biological feed by apple pomace microbial culture starter
CN101690540A (en) Method for preparing composite protein feed by mixed fermentation
CN106376725A (en) Biological fermented feed and preparation method thereof
CN103598415B (en) Sunflower seed meal microbial protein feed and preparation method thereof
CN103468594B (en) Candidautilis strain and application thereof
CN105494890A (en) Enzymolysis and fermentation combined method for improving feeding nutritional value of rapeseed meal
CN106173225A (en) A kind of method that solid fermentation Plant protein feed prepares additive of protein feed
CN106222114B (en) The bacillus of efficient degradation dregs of beans antigen protein and the method for bacterium enzyme mixed fermentation
CN107455578A (en) The method that cattle and sheep ensilage is produced using waste vegetable leaves as raw material
CN103535525B (en) Production method of biological feed additive rich in amino acids and proteins
CN103355477B (en) Production method for feed through fermentation of soy sauce residues
CN104161172A (en) Method for producing probiotic peptides by using two-step solid-state fermentation of peanut meal
CN103960463A (en) Silage tangerine pulp feed and preparation technique thereof
CN104872376A (en) Method for preparing probitics through two-step fermentation method by using degreased rice bran as raw material
CN107373034A (en) Using waste vegetable leaves as raw material production chicken, the method for duck feed
CN106551132A (en) A kind of fermentative feedstuff of microbe preparation method that can improve pork quality
CN106260661A (en) A kind of fermentative feedstuff of microbe manufacture method that can improve pork quality

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
C14 Grant of patent or utility model
GR01 Patent grant