CN104605138A - Poultry feed additive containing fermented Chinese yew, and preparation method and applications thereof - Google Patents
Poultry feed additive containing fermented Chinese yew, and preparation method and applications thereof Download PDFInfo
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- CN104605138A CN104605138A CN201310535958.6A CN201310535958A CN104605138A CN 104605138 A CN104605138 A CN 104605138A CN 201310535958 A CN201310535958 A CN 201310535958A CN 104605138 A CN104605138 A CN 104605138A
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Abstract
The invention belongs to the field of biological feed, and relates to a poultry feed additive containing fermented Chinese yew, and a preparation method and applications thereof. The poultry feed additive is a dried product obtained via fermentation of Chinese yew using a compound microbial agent, and the compound microbial agent is composed of lactobacillus plantarum fermentation broth, lactobacillus acidophilus fermentation broth, and candida utilis fermentation broth. The compound microbial agent is used for biodegradation of active ingredients of Chinese yew; treatment method is mild; the active ingredients of Chinese yew are protected as far as possible; poultry absorption rate is increased; a poultry feed prepared from the poultry feed additive contains Chinese yew ingredient, lactic acid bacteria, and saccharomycetes, and is capable of increasing poultry production efficiency; a Chinese yew feed obtained via microbial fermentation is mixed with poultry daily feed at a ratio of 1 to 5%, and an obtained mixture can be used for feeding after uniform stirring.
Description
Technical field
The invention belongs to biological feedstuff field, relate to a kind of livestock fodder additives containing fermentation Chinese yew and its preparation method and application.
Background technology
Chinese yew is one of plants for anticancer studying at most in world wide in recent years.Find (mainly at bark) anti-cancer active matter containing mechanism of action uniqueness in Chinese yew body after deliberation, wherein the strongest with the activity of taxol, be the autonomic drug uniquely promoting microtubule polymerization now, suppress tubulin depolymerization, its medical value is widely deployed.At the initial stage, the heavy demand of pharmaceuticals industry to taxol result in Chinese yew by a large amount of artificial cultivation, but due to the maturation of taxol chemical synthesising technology in recent years, the price of its plant extracts reduces again and again, and the Chinese yew of many artificial growths becomes landscape tree.
For expanding the development and utilization level of Chinese yew further, the effect of its each effective constituents of ingredient exerts.Chinese yew, by the way of fermentable process, is made poultry feed by the present invention.Existing part researcher has carried out the Primary Study of Chinese yew feed at present, if number of patent application is disclose one in " nano-taxaceae series poultry feed and preparation method thereof " of 201110191928.9 to utilize pure physical method that taxus chinensis powder is broken to nanoscale, then mix with poultry feed; And for example that to be 201010153961.8 disclose in " a kind of organic feeds containing taxol component with anticancer function and preparation method thereof " number of patent application is a kind of using the method as animal feed after Chinese yew, pteridophyte mix in proportion.Chinese yew animal feed involved by above-mentioned patent all adopts physics way to prepare, and does not relate to any biological treatment means, relies on animal digestion to absorb the active ingredient of Chinese yew completely.
Summary of the invention
The object of the invention is the above-mentioned deficiency for prior art, a kind of livestock fodder additives containing fermentation Chinese yew is provided.
Another object of the present invention is to provide the preparation method of this feed addictive.
Another object of the present invention is to provide the application of this feed addictive.
Object of the present invention realizes by following technical scheme:
A kind of livestock fodder additives containing fermentation Chinese yew, for Chinese yew through complex micro organism fungicide fermentation and through wheat bran absorption after dry thing, wherein said complex micro organism fungicide is made up of Lactobacillus plantarum (Lactobacillusplantarum) zymotic fluid, lactobacillus acidophilus (Lactobacillus acidophilus) zymotic fluid, candida utili (Candida utilis) zymotic fluid.
In described complex micro organism fungicide, preferably the zymotic fluid living bacteria count of 3 kinds of bacterium is distinguished all 10
9more than individual/milliliter, in the complex micro organism fungicide formed after mixing, the living bacteria count of 3 kinds of bacterium is all not less than 5 × 10 respectively
7individual/milliliter.
The preferred branches and leaves of yew of described Chinese yew.
The described livestock fodder additives containing fermentation Chinese yew prepares preferably by following methods:
(1) Chinese yew is first through 40 ~ 65 DEG C of oven dry, and then is crushed to 50 ~ 80 orders;
(2) prepare its zymotic fluid according to the normal fermentation of 3 kinds of microorganisms, and be mixed with into complex micro organism fungicide;
(3) the Chinese yew powder after pulverizing is added in complex micro organism fungicide zymotic fluid, dosage is 150 ~ 250g Chinese yew powder/L zymotic fluid, add 8 ~ 15g/L brown sugar and 10 ~ 20g/L peptone simultaneously, after mixing, at 30 DEG C, 100r/min condition bottom fermentation 15 ~ 18 hours, when zymotic fluid pH lower than 5.0 time, fermentation ends;
(4) adsorb by adding wheat bran in zymotic fluid the Chinese yew solid content obtained containing thalline, solid content is through 50 DEG C of oven dry;
(5) the Chinese yew solid content of gained is dried through pulverizing and obtain the described livestock fodder additives containing fermentation Chinese yew.
The preparation method of the livestock fodder additives containing fermentation Chinese yew of the present invention, comprises following steps:
(1) Chinese yew is first through 50 DEG C of oven dry, and then is crushed to 50 orders;
(2) prepare its zymotic fluid according to the normal fermentation of 3 kinds of microorganisms, and be mixed with into complex micro organism fungicide, in the complex micro organism fungicide wherein formed after mixing, the living bacteria count of 3 kinds of bacterium is all not less than 5 × 10 respectively
7individual/milliliter;
(3) the Chinese yew powder after pulverizing is added in complex micro organism fungicide zymotic fluid, dosage is 150 ~ 250g Chinese yew powder/L zymotic fluid, add 8 ~ 15g/L brown sugar and 10 ~ 20g/L peptone simultaneously, after mixing, at 30 DEG C, 100r/min condition bottom fermentation 15 ~ 18 hours, when zymotic fluid pH lower than 5.0 time, fermentation ends;
(4) adsorb by adding wheat bran in zymotic fluid the Chinese yew solid content obtained containing thalline, solid content is through 50 DEG C of oven dry;
(5) the Chinese yew solid content of gained is dried through pulverizing and obtain the described livestock fodder additives containing fermentation Chinese yew.
Wherein, described Chinese yew is branches and leaves of yew.
Livestock fodder additives of the present invention is preparing the application in poultry feed.
A kind of poultry feed, adds the livestock fodder additives gained containing fermentation Chinese yew of the present invention according to the ratio of 1% ~ 5% in the daily feed of poultry.
Microorganism used therefor bacterial strain of the present invention is all included in The Ministry of Agriculture of the People's Republic of China, MOA " feed addictive kind catalogue ", meets national pertinent regulation system.The special requirement in the source of three kinds of microorganisms, Lactobacillus plantarum that is commercially available or each large Spawn preservation organization, lactobacillus acidophilus, candida utili are all applicable to the present invention.
Beneficial effect:
The present invention is by the organic assembling to the microbial strains of 3 kinds of excellent performances, make complex micro organism fungicide, this complex micro organism fungicide has carried out biodegradation to active ingredient in Chinese yew, wherein in Chinese yew, content of cellulose reduces, polysaccharide in fermentation liquid content increases, and can improve poultry absorptivity.
Poultry feed made by the present invention, both containing Chinese yew composition, again containing lactic acid bacteria and saccharomycete two class probio, can improve the absorbability of animal to Chinese yew active ingredient, again can probiotic supplemented.After both being combined, effectively can promote the production efficiency of poultry animal.
The present invention is gentle to Chinese yew processing mode, can at utmost retain its active ingredient.
The Chinese yew that the present invention utilizes is branches and leaves of yew.In Chinese yew cultivating process, the discarded branches and leaves pruned are become poultry feed, turn waste into wealth by the regular trimming leaf of meeting after microbiological treatment, economical and practical.
Detailed description of the invention
Below in conjunction with embodiment, the present invention is elaborated, in embodiment for describe in detail all by state of the art
The preparation of embodiment 1 zymotic fluid
The present embodiment is only described in detail for Lactobacillus plantarum (ACCC11016), lactobacillus acidophilus (ACCC03955), the particular content of candida utili (ACCC20060) to invention, not to limit in complex micro organism fungicide of the present invention three kinds of microbe-derived selections.
(1) above Lactobacillus plantarum (ACCC11016), lactobacillus acidophilus (ACCC03955), candida utili (ACCC20060) are activated to respective preservation slant medium respectively, 28 ?30 DEG C cultivate 24 ~ 48 hours.
The preservation slant medium formula of three kinds of microorganisms is as follows:
Lactobacillus plantarum and lactobacillus acidophilus all adopt MRS culture medium: casein peptone 10.0 grams, beef extract 10.0 grams, yeast extract 5.0g, glucose 5.0g, sodium acetate 5.0 grams, citric acid diamino 2.0 grams, tween Tween801.0 gram, K
2hPO
42.0 grams, MgSO47H
2o0.02 gram, MnSO
4h
2o0.05 gram, 15 grams, agar, 1000 milliliters, running water, pH6.8;
Candida utili adopts culture medium: 100 grams, potato, glucose 5 grams, yeast extract 5 grams, peptone 2 grams, 15 grams, agar, 1000 milliliters, running water, pH nature.
(2) be inoculated in respective fluid nutrient medium respectively by the bacterial classification activated, 28 ?30 DEG C, 110 revs/min of cultivation 18 ?24 are little of exponential phase, obtain seed liquor.
The Liquid Culture based formulas of three kinds of microorganisms is filled a prescription identical with slant medium, does not need to add agar in process for preparation.
(3) fermentation tank 200 liters, charge 150 liters, feed intake by above-mentioned 3 kinds of microbial liquid culture medium prescriptions respectively, then 121 DEG C of sterilizings 20 minutes, add 4 after cooling respectively and go up the seed liquor stated and be cultured to logarithmic phase, the fermentation condition of each bacterial strain is respectively: Lactobacillus plantarum and lactobacillus acidophilus, fermented and cultured temperature 35 DEG C, rotating speed 90 revs/min, stuffiness, incubation time 48 hours; Candida utili, fermented and cultured temperature 30 DEG C, rotating speed 150 revs/min, throughput 1:1.2(and fermentating liquid volume ratio), incubation time 24 hours.
In the zymotic fluid of above 3 kinds of bacterium, living bacteria count is respectively all 10
9more than individual/milliliter.
Embodiment 2 is fermented containing the present invention the poultry feed of Chinese yew livestock fodder additives
1, three kinds of microbial fermentation solutions that prepared by embodiment 1 mix by following proportioning and become complex micro organism fungicide: Lactobacillus plantarum zymotic fluid 8L, lactobacillus acidophilus zymotic fluid 10L, candida utili zymotic fluid 12L, add distilled water to 50L after mixing.Wherein the living bacteria count of 3 kinds of bacterium is all not less than 5 × 10 respectively
7individual/milliliter.
2, select and be crushed to 50 object branches and leaves of yew powder 10kg and join in bacterial classification retort, measuring its content of cellulose is 38.2g/g dry powder;
3, in retort, add complex micro organism fungicide 50L prepared by step 1, add 500g brown sugar and 750g peptone, after mixing, measuring its polyoses content is 13.2mg/L simultaneously, at 30 DEG C, and 100r/min condition bottom fermentation 18 hours;
4, measuring zymotic fluid pH is 4.8, stops fermentation, and measuring polysaccharide in fermentation liquid content is 89.7mg/L, and in zymotic fluid, the content of cellulose of solid content is 32.4g/g dry powder.The sediment that wheat bran absorption obtains Chinese yew and thalline is added in zymotic fluid;
5, sediment is placed in baking oven, dries rear pulverizing for 50 DEG C.
6, the solid content 5kg taken after pulverizing in step 5 adds the daily feed of 100kg poultry (corn 64%, dregs of beans 22%, cotton dregs 5%, fish meal 2%, stone flour 6.7%, salt 0.3%) in stir, obtain the poultry feed of the Chinese yew livestock fodder additives that to ferment containing the present invention.
Comparative example 1
After meal, air-flow crushing, nanometer pulverization, made by branches and leaves of yew its average grain diameter reach 100 ~ 300nm, nano-taxus powder 2Kg is added in the daily feed of 100kg poultry and stirs, obtain the poultry feed containing nano-taxus.
Comparative example 2
1) 5kg bark of Ramulus et folium taxi cuspidatae is pulverized by the specification that length is 1 ~ 2cm;
2) dried by 45kg Azolla imbricata, the specification being 1 ~ 2cm by length is pulverized;
3) by step 1) and step 2) gained pulverize after Chinese yew and Azolla imbricata mixing;
4) by step 3) mixture with and 50kg auxiliary material (mixture of wheat bran, wheat and sugarcane stalk and rape) mix and obtain a kind of organic feed containing taxol component with anticancer function in 201010153961.8.
Contrast effect example
Choose 1200 25 week age laying hen test, be divided into 4 groups at random, often organize 300, all laying hens are positioned at samely raises chicken house scattered, with the isolation of purse seine subregion, ensures that experiment laying hen is not intersected.Use feed made by embodiment 1, comparative example 1, comparative example 2 and the daily forage feed of poultry respectively.Other carry out feeding and management routinely, free choice feeding and drinking-water.Experiment lasts 45 days.Chicken is raised by special messenger in experiment, free choice feeding, drinking-water, gravity-flow ventilation, illumination every day 17h, and day feeds 2 times.Record egg number every day, egg size, add up laying rate weekly.Experimental result is in table 1.As can be seen from Table 1, when feed consumption is suitable, each compared with all the other group of embodiment 1 egg production all has remarkable increase, and wherein more daily feed group improves 4.68, and comparatively comparative example improves 4.29% and 3.91% respectively.
Table 1 contrast and experiment
Claims (8)
1. the livestock fodder additives containing fermentation Chinese yew, it is characterized in that for Chinese yew through complex micro organism fungicide fermentation and through wheat bran absorption after dry thing, wherein said complex micro organism fungicide is made up of Lactobacillus plantarum (Lactobacillus plantarum) zymotic fluid, lactobacillus acidophilus (Lactobacillus acidophilus) zymotic fluid, candida utili (Candida utilis) zymotic fluid.
2. livestock fodder additives according to claim 1, is characterized in that the zymotic fluid living bacteria count of 3 kinds of bacterium in described complex micro organism fungicide is respectively all 10
9more than individual/milliliter, in the complex micro organism fungicide formed after mixing, the living bacteria count of 3 kinds of bacterium is all not less than 5 × 10 respectively
7individual/milliliter.
3. livestock fodder additives according to claim 1, is characterized in that described Chinese yew is branches and leaves of yew.
4. the livestock fodder additives according to any one of claims 1 to 3, is characterized in that the described livestock fodder additives containing fermentation Chinese yew prepares by the following method:
(1) Chinese yew is first through 40 ~ 65 DEG C of oven dry, and then is crushed to 50 ~ 80 orders;
(2) prepare its zymotic fluid according to the normal fermentation of 3 kinds of microorganisms, and be mixed with into complex micro organism fungicide;
(3) the Chinese yew powder after pulverizing is added in complex micro organism fungicide zymotic fluid, dosage is 150 ~ 250g Chinese yew powder/L zymotic fluid, add 8 ~ 15g/L brown sugar and 10 ~ 20g/L peptone simultaneously, after mixing, at 30 DEG C, 100r/min condition bottom fermentation 15 ~ 18 hours, when zymotic fluid pH lower than 5.0 time, fermentation ends;
(4) adsorb by adding wheat bran in zymotic fluid the Chinese yew solid content obtained containing thalline, solid content is through 50 DEG C of oven dry;
(5) the Chinese yew solid content of gained is dried through pulverizing and obtain the described livestock fodder additives containing fermentation Chinese yew.
5. the preparation method of the livestock fodder additives containing fermentation Chinese yew according to claim 1, is characterized in that comprising following steps:
(1) Chinese yew is first through 40 ~ 65 DEG C of oven dry, and then is crushed to 50 ~ 80 orders;
(2) prepare its zymotic fluid according to the normal fermentation of 3 kinds of microorganisms, and be mixed with into complex micro organism fungicide, in the complex micro organism fungicide wherein formed after mixing, the living bacteria count of 3 kinds of bacterium is all not less than 5 × 10 respectively
7individual/milliliter;
(3) the Chinese yew powder after pulverizing is added in complex micro organism fungicide zymotic fluid, dosage is 150 ~ 250g Chinese yew powder/L zymotic fluid, add 8 ~ 15g/L brown sugar and 10 ~ 20g/L peptone simultaneously, after mixing, at 30 DEG C, 100r/min condition bottom fermentation 15 ~ 18 hours, when zymotic fluid pH lower than 5.0 time, fermentation ends;
(4) adsorb by adding wheat bran in zymotic fluid the Chinese yew solid content obtained containing thalline, solid content is through 50 DEG C of oven dry;
(5) the Chinese yew solid content of gained is dried through pulverizing and obtain the described livestock fodder additives containing fermentation Chinese yew.
6. preparation method according to claim 5, is characterized in that described Chinese yew is branches and leaves of yew.
7. livestock fodder additives according to claim 1 is preparing the application in poultry feed.
8. a poultry feed, is characterized in that adding the livestock fodder additives gained containing fermentation Chinese yew according to claim 1 according to the ratio of 1% ~ 5% in the daily feed of poultry.
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Cited By (5)
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| CN108815208A (en) * | 2018-04-02 | 2018-11-16 | 孙向勇 | Health care fermentation wood powder |
| WO2022162101A1 (en) * | 2021-01-28 | 2022-08-04 | Phyto-Technics Bv | Production of taxol from taxus cuttings |
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