CN106578645A - Poultry feed additive containing Taxus chinensis, preparation method thereof and prepared poultry feed - Google Patents
Poultry feed additive containing Taxus chinensis, preparation method thereof and prepared poultry feed Download PDFInfo
- Publication number
- CN106578645A CN106578645A CN201611200283.XA CN201611200283A CN106578645A CN 106578645 A CN106578645 A CN 106578645A CN 201611200283 A CN201611200283 A CN 201611200283A CN 106578645 A CN106578645 A CN 106578645A
- Authority
- CN
- China
- Prior art keywords
- bacillus
- parts
- bacterium solution
- ramulus
- fodder additives
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Landscapes
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Fodder In General (AREA)
Abstract
The invention relates to a poultry feed additive containing Taxus chinensis, a preparation method thereof and a prepared poultry feed. The poultry feed additive comprises a mixture obtained by respectively mixing a plurality of probiotics bacteria liquids with a Taxus chinensis solid medium. The poultry feed additive has the advantages that the Taxus chinensis resource is fully used, production equipment investment is little, energy consumption is low, the technology is simple and feasible; matrix water content is low, volume of a bioreactor is greatly reduced, waste water processing is not required, environment pollution is little, the post-treatment is convenient; and strict aseptic technique during a production process is not required, the method is easy to popularize and use, and provides a novel approach for effective utilization of the Taxus chinensis resource. The poultry feed can generate beneficial effect on poultry production and disease-resistant conditions, the cultured poultry as a food can be the effective component of paclitaxel which is required by human body, and the function of the feed enables further research.
Description
Technical field
The invention belongs to biological fermentation feed field, and in particular to a kind of livestock fodder additives containing Ramulus et folium taxi cuspidatae, its system
Preparation Method and its poultry feed of preparation.
Background technology
Ramulus et folium taxi cuspidatae is one of plants for anticancer most studied in world wide in recent years.It has been investigated that in Ramulus et folium taxi cuspidatae body
Anti-cancer active matter containing mechanism of action uniqueness, wherein it is most strong with the activity of paclitaxel, to become cancer therapy drug；But
As Ramulus et folium taxi cuspidatae artificial culture amount is big, paclitaxel chemical synthesising technology is ripe, and plant extract market reduces, and is fully to develop
Ramulus et folium taxi cuspidatae, plays its each effective constituents effect, therefore the present invention is by microbial treatments, as livestock fodder additives.
Solid fermentation (Solid-state fermentation, abbreviation SSF) to be referred to and do carbon source and energy using nature substrate
Source, or do solid support using inert substrate, its system are anhydrous or close to anhydrous any sweat, solid fermentation skill
Art develops for a long time, is widely used.China just has been applied in the aspects such as yeast production wine brewing, cure foods, fertilizer accumulation in ancient times.
Limited by development in science and technology, solid-fermented technique all rests on the original backwardness of comparison within past a very long time
State, or even still such fermentation technique is being continued to use in modern commercial production.Nearest two during the decade, send out with regard to solid
The research and application of ferment technology is developed rapidly, and people are in the research in solid fermentation field and its in resource environment, albumen
Application in matter feedstuff achieves progress, is mainly manifested in biological feedstuff, bio-fuel, biological pesticide, bioconversion, biological solution
The application of the aspect such as poison and biological restoration.There is presently no patent utilization microorganism and Ramulus et folium taxi cuspidatae adopts solid-fermented technique, increase
Plus the content of taxus brevifolia alcohol.
Probiotic bacteria：Derive from Greek earliest, mean " beneficial to life ", referring to improve host (such as animals or humans)
The balance of enteral Tiny ecosystem, and have the active microorganism of front benefit to host, with alleviation lactase deficiency shape, prevention reproduction
System infections, enhancing body immunity, reduce serum cholesterol, help absorb nutritional labeling, strengthen the work such as plant metabolism
With.In human body, animal body, beneficial antibacterial or funguses mainly have clostridium butyricum, lactic acid bacteria, bacillus bifiduss, bacillus acidophilus, put
Line bacterium, yeast etc..But the relevant report how to be combined probiotic bacteria and Ramulus et folium taxi cuspidatae effective ingredient is also more rare at present.
The content of the invention
The invention aims to providing one kind can improve the poultry speed of growth and immunity of poultry, production effect is improved
The poultry feed of the livestock fodder additives containing Ramulus et folium taxi cuspidatae, its preparation method and its preparation of rate.
For achieving the above object, the solution of present invention offer is：Livestock fodder additives bag containing Ramulus et folium taxi cuspidatae composition
Include following component：Including various probiotics bacterial liquids mixture respectively with the tunning of Ramulus et folium taxi cuspidatae solid medium.
Further, in above-mentioned livestock fodder additives, the Ramulus et folium taxi cuspidatae solid medium includes following content of component：
10～20 parts of Ramulus et folium taxi cuspidatae branch powders, 60～70 parts of wheat brans, 5～10 parts of rice husks, 5～10 parts of Semen Maydis powder, 5～10 parts of bean cake,
0.05～0.1 part of magnesium sulfate and 0.01～0.02 part of manganese sulfate.
Further, in above-mentioned livestock fodder additives, various probiotics bacterial liquids include：Bacillus subtilises bacterium
Liquid, bacillus acidophilus' bacterium solution and discrimination bacillus bacterium solution.
Further, in above-mentioned livestock fodder additives, the water content of the Ramulus et folium taxi cuspidatae solid medium is 50-55%.
Further, in above-mentioned livestock fodder additives, in the tunning, prebiotic bacterium solution includes following mass parts group
Point：30～40 parts of bacillus subtilises；30～40 parts of bacillus acidophilus；30～40 parts of bacillus bifiduss；Various probiotics bacterials
Liquid with the usage ratio of Ramulus et folium taxi cuspidatae solid medium is：300ml bacterium solutions/1kg solid mediums.
By compounding suitable bacterium solution and Ramulus et folium taxi cuspidatae solid medium, meet the edible preference of poultry so that using a small amount of
Additive, can effectively improve the poultry speed of growth, improve immunity of poultry, by with the culture of Ramulus et folium taxi cuspidatae solid medium benefit
Raw bacterium so that Ramulus et folium taxi cuspidatae effective ingredient is capable of the activity of its effective ingredient of storage life to the full extent by probiotic bacteria organic absorbent.
Additionally, the inventive method ecological, environmental protective, for the harm of poultry drug residue free, the meat productss and egg products safety of processing of poultry
It is reliable, and contain probioticss and Ramulus et folium taxi cuspidatae effective ingredient, can be into health food edible for humans.By various strains respectively with it is solid
The mixing of body culture medium carries out the quality that solid fermentation can greatly improve compound bacteria, so as to improve the raising of the poultry feed of preparation
Poultry production and resistances against diseases.
The preparation method of above-mentioned livestock fodder additives includes step：
Bacillus subtilis strain is seeded to cultivate in fluid medium bacillus subtilises bacterium solution is obtained, by acidophilus breast
Culture during bacillus species are seeded to MRS culture medium is obtained bacillus acidophilus' bacterium solution, and bifidobacterium species are seeded to MRS culture medium
Middle culture is obtained bacillus bifiduss bacterium solution, and above-mentioned bacterium solution is mixed homogeneously with Ramulus et folium taxi cuspidatae solid medium respectively, under the conditions of 37 DEG C, quiet
Co-grinding after culture 36h lyophilizations is put, product is obtained final product.
Actually above-mentioned bacterium solution is mixed with Ramulus et folium taxi cuspidatae solid medium again and is cultivated after can also mixing, but by above-mentioned bacterium
Liquid is mixed homogeneously with Ramulus et folium taxi cuspidatae solid medium respectively to carry out cultivating the content and matter that can greatly improve viable bacteria in final products
Amount.
Further, in the preparation method of above-mentioned livestock fodder additives, the bacillus subtilises bacterium solution is by such as lower section
It is prepared by method：
A. component is mixed by mass parts：2～5 parts of glucoses, 0.2～0.8 part of Sodium Chloride, 0.2～0.8 part of yeast extract and
Mixture pH is adjusted to 7.0 by 1～2 part of peptone；
B. Bacillus subtilis strain is seeded in sterilized fluid medium, liquid amount 20%, culture medium as
37 DEG C of constant-temperature table 220r/min, cultivate 24h, obtain final product bacillus subtilises bacterium solution.
Further, in the preparation method of above-mentioned livestock fodder additives, bacillus acidophilus' bacterium solution is as follows
Prepare：
Lactobacillus acidophilus species are seeded in MRS culture medium, liquid amount 20%, to 37 DEG C of constant-temperature table 220r/min,
Culture 24h, obtains final product bacillus acidophilus' bacterium solution.
Livestock fodder additives according to claim 6, it is characterised in that：The bacillus bifiduss bacterium solution is by such as lower section
It is prepared by method：
Bifidobacterium species are seeded in MRS culture medium, liquid amount 20%, to 37 DEG C of constant-temperature table 220r/min, training
Foster 24h, obtains final product bacillus bifiduss bacterium solution.
The present invention also provides a kind of poultry feed, and which includes following parts by weight of component：It is 98～92 parts of feedstuff, as above
2～8 parts of livestock fodder additives.
The livestock fodder additives containing Ramulus et folium taxi cuspidatae provided using the present invention, can be processed to Ramulus et folium taxi cuspidatae or effective ingredient is carried
Leftover pieces after taking are utilized, and are sufficiently used taxus resource, and small investment of production equipment, energy consumption are low, and technology is simpler
It is feasible；Water content of substrate is low, can greatly reduce the volume of bioreactor, it is not necessary to which wastewater treatment, environmental pollution are less, locate afterwards
Reason is easy to process；Production process does not need strict sterile working；Method is easily promoted the use of, the effective profit to taxus resource
With there is provided new approach.
The poultry feed that the present invention is prepared can produce beneficial effect to the production of poultry and disease-resistant aspect, what which was raised out
Poultry becomes food and becomes one of mode of human body acquisition paclitaxel effective ingredient, currently further studies which to human body
Effect.
Specific embodiment
With reference to specific embodiment, the present invention will be further described.It should be noted that the reality described in the present invention
It is only the preferred embodiments of the present invention to apply example, is not limited to the present invention, for a person skilled in the art, this
Invention can have various modifications and variations.All any modifications within the spirit and principles in the present invention, made, equivalent,
Improve etc., should be included within the scope of the present invention.Based on the embodiment in the present invention, those of ordinary skill in the art
The every other embodiment obtained under the premise of creative work is not made, belongs to the scope of protection of the invention.
The preparation of strain liquid
The present embodiment is only with bacillus subtilises (ACCC01185), bacillus acidophilus (ACCC03955), bacillus bifiduss
(ACCC11054) invention can be held as a example by and describes in detail, be not intended to limit strain source.
(1) by bacillus subtilises (ACCC01185), bacillus acidophilus (ACCC03955), bacillus bifiduss
(ACCC11054) activate respectively to regulation preservation slant medium, 30 DEG C of 24～36h of culture.
The storage medium and fermentation medium of three kinds of strains is as follows：
Bacillus subtilises (ACCC01185) storage medium and fermentation medium：Glucose 0.5%；Nacl0.5%；
Yeast extract 0.8%；Peptone 1%；Agar 2%；PH=7.0；
What bacillus acidophilus (ACCC03955), bacillus bifiduss (ACCC11054) storage medium and fermentation medium were used
It is MRS culture medium；MRS culture medium：Peptone 10.0g；Beef powder 5.0g yeast powder 4.0g；Glucose 20.0g；Tween 80
1.0mL；Dipotassium hydrogen phosphate 2.0g；Sodium acetate 5.0g；Triammonium citrate 2.0g；Magnesium sulfate 0.2g；Manganese sulfate 0.05g；Agar powder
15.0g；Distilled water 1000mL；
The fermentation medium of three kinds of strains is not added with agar for above formula.
(2) strain for having activated is inoculated in respective fluid medium respectively, liquid amount 20%, 37 DEG C, 220r/min
24h is to exponential phase for culture, obtains seed liquor, i.e. bacterium solution.
It is prepared by one solid fermentation of embodiment and additive
(1) Ramulus et folium taxi cuspidatae solid medium is prepared：Ramulus et folium taxi cuspidatae branch powder 20%, wheat bran 60%, rice husk 10%, Semen Maydis powder
5%th, bean cake 5%, magnesium sulfate 0.05%, manganese sulfate 0.01%, have weighed each component, and mix homogeneously.
(2), after above-mentioned solid medium mix homogeneously, a small amount of water-wet culture medium is added, then is separately added into fresh training
The bacterium solution supported, mix homogeneously so as to which water content is maintained at 50-55%, postvaccinal culture medium are laid in triangular flask, 37
Under the conditions of DEG C, quiescent culture 36h；
(3) by the product after culture, through lyophilization, remove excessive moisture and then crush.
(4) each tunning powder is according to bacillus subtilis (Ehrenberg) Cohn fermented product powder 40%, bacillus acidophilus' tunning
Powder 30%, the ratio mix homogeneously of bifidobacterium fermentation powder product 30%, obtains final product feed additive powder.
(5) additive powder is added in poultry conventional feed according to 5% addition, it is normal to feed.
Comparative example one
Ramulus et folium taxi cuspidatae branch is ground into into coarse powder, daily feeding is added into by 1% (Ramulus et folium taxi cuspidatae point content in additive) addition
It is in material, normal to feed；
Comparative example two
By Ramulus et folium taxi cuspidatae branch powder 20%, wheat bran 60%, rice husk 10%, Semen Maydis powder 5%, bean cake 5%, consist of fermentation and add
Plus agent, it is added in the daily culture medium of poultry by 5% addition, it is normal to feed.
Contrast effect
Selection company chicken house 400 30 weeks is randomly divided into 4 groups just in hen laying eggs as sample, and per group is fed respectively
In individual region, in addition to additive difference, other rearing conditions are unanimously tested, and are fed 60 days.Obtain following table result：
| Title | Egg quantity (individual) | Feedstuff consumption (kg) | Death toll (only) |
| Additive group | 4320 | 450 | 0 |
| One group of comparative example | 3905 | 405 | 2 |
| Two groups of comparative example | 4012 | 412 | 1 |
| It is not added with group | 3950 | 403 | 1 |
Claims (10)
1. a kind of livestock fodder additives containing Ramulus et folium taxi cuspidatae composition, it is characterised in that：Including various probiotics bacterial liquids respectively with it is red
The mixture of the tunning of bean China fir solid medium.
2. livestock fodder additives according to claim 1, it is characterised in that：The Ramulus et folium taxi cuspidatae solid medium include as
Lower content of component：10～20 parts of Ramulus et folium taxi cuspidatae branch powders, 60～70 parts of wheat brans, 5～10 parts of rice husks, 5～10 parts of Semen Maydis powder, 5
～10 parts of bean cake, 0.05～0.1 part of magnesium sulfate and 0.01～0.02 part of manganese sulfate.
3. livestock fodder additives according to claim 1, it is characterised in that：Various probiotics bacterial liquids include：It is withered
Careless bacillus cereuss bacterium solution, bacillus acidophilus' bacterium solution and discrimination bacillus bacterium solution.
4. livestock fodder additives according to claim 1, it is characterised in that：The Ramulus et folium taxi cuspidatae solid medium it is aqueous
Measure as 50-55%.
5. livestock fodder additives according to claim 1, it is characterised in that：In the tunning probiotic bacteria include as
Lower content of component：30～40 parts of bacillus subtilises；30～40 parts of bacillus acidophilus；30～40 parts of bacillus bifiduss；It is described many
Planting probiotics bacterial liquid with the usage ratio of Ramulus et folium taxi cuspidatae solid medium is：300ml bacterium solutions/1kg solid mediums.
6. the preparation method of the livestock fodder additives as described in any one in claim 1 to 5, it is characterised in that：Including
Step：
Bacillus subtilis strain is seeded to cultivate in fluid medium bacillus subtilises bacterium solution is obtained, by bacillus acidophilus
Culture during strain is seeded to MRS culture medium is obtained bacillus acidophilus' bacterium solution, bifidobacterium species is seeded in MRS culture medium and is trained
Prepared bacillus bifiduss bacterium solution is supported, above-mentioned bacterium solution is mixed homogeneously with Ramulus et folium taxi cuspidatae solid medium respectively, under the conditions of 37 DEG C, stand training
After foster 36h, co-grinding after lyophilization, obtains final product product.
7. the preparation method of livestock fodder additives according to claim 6, it is characterised in that：The bacillus subtilises
Bacterium solution is prepared as follows：
A. component is mixed by mass parts：2～5 parts of glucoses, 0.2～0.8 part of Sodium Chloride, 0.2～0.8 part of yeast extract and 1～2
Mixture pH is adjusted to 7.0 by part peptone；
B. Bacillus subtilis strain is seeded in sterilized fluid medium, liquid amount 20%, culture medium is as 37 DEG C
Constant-temperature table 220r/min, cultivates 24h, obtains final product bacillus subtilises bacterium solution.
8. the preparation method of livestock fodder additives according to claim 6, it is characterised in that：The bacillus acidophilus bacterium
Liquid is prepared as follows：
Lactobacillus acidophilus species are seeded in MRS culture medium, liquid amount 20%, to 37 DEG C of constant-temperature table 220r/min, culture
24h, obtains final product bacillus acidophilus' bacterium solution.
9. livestock fodder additives according to claim 6, it is characterised in that：The bacillus bifiduss bacterium solution is as follows
Prepare：
Bifidobacterium species are seeded in MRS culture medium, liquid amount 20%, to 37 DEG C of constant-temperature table 220r/min, culture
24h, obtains final product bacillus bifiduss bacterium solution.
10. a kind of poultry feed, it is characterised in that：Including following parts by weight of component：98～92 parts of feedstuff, such as claim 1 to 9
2～8 parts of livestock fodder additives described in middle any one.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611200283.XA CN106578645A (en) | 2016-12-22 | 2016-12-22 | Poultry feed additive containing Taxus chinensis, preparation method thereof and prepared poultry feed |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611200283.XA CN106578645A (en) | 2016-12-22 | 2016-12-22 | Poultry feed additive containing Taxus chinensis, preparation method thereof and prepared poultry feed |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106578645A true CN106578645A (en) | 2017-04-26 |
Family
ID=58600842
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201611200283.XA Pending CN106578645A (en) | 2016-12-22 | 2016-12-22 | Poultry feed additive containing Taxus chinensis, preparation method thereof and prepared poultry feed |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106578645A (en) |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102870973A (en) * | 2011-07-11 | 2013-01-16 | 粟时献 | Nano-Taxus poultry feed and its manufacturing method |
| CN103444619A (en) * | 2012-05-28 | 2013-12-18 | 浙江百年红豆杉生物科技有限公司 | Chicken raising method by utilizing Taxus chinensis wood and Taxus chinensis feed |
| CN104605138A (en) * | 2013-11-01 | 2015-05-13 | 南京黑玉特种家禽养殖专业合作社 | Poultry feed additive containing fermented Chinese yew, and preparation method and applications thereof |
-
2016
- 2016-12-22 CN CN201611200283.XA patent/CN106578645A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102870973A (en) * | 2011-07-11 | 2013-01-16 | 粟时献 | Nano-Taxus poultry feed and its manufacturing method |
| CN103444619A (en) * | 2012-05-28 | 2013-12-18 | 浙江百年红豆杉生物科技有限公司 | Chicken raising method by utilizing Taxus chinensis wood and Taxus chinensis feed |
| CN104605138A (en) * | 2013-11-01 | 2015-05-13 | 南京黑玉特种家禽养殖专业合作社 | Poultry feed additive containing fermented Chinese yew, and preparation method and applications thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102093967B (en) | Mink source enterococcus faecium and application thereof | |
| CN102586155B (en) | Lactobacillus plantarum N13 and use thereof | |
| CN104962490B (en) | A kind of Enteromorpha microbial bacterial agent and preparation method thereof | |
| CN103749966B (en) | Potato residues fermented feed and preparation method thereof | |
| CN103088010A (en) | Biological health padding and preparation method thereof | |
| CN109022333A (en) | A kind of preparation method and applications of composite microbial fermentation bacteria agent | |
| CN106011001A (en) | Livestock intestinal ecological restoration microbial inoculum with antiviral and antibacterial effects and capable of replacing antibiotics and preparation method of livestock intestinal ecological restoration microbial inoculum | |
| CN108977397A (en) | A kind of bacillus solid formulation that feeding compound selenium-rich is prebiotic and preparation method | |
| CN104719635A (en) | Method for preparing feed additive mixed and fermented by aquatic product leftover and Chinese herbal medicine | |
| CN104855684A (en) | An antibacterial traditional Chinese medicine microbial fermentation preparation and a preparation method and application thereof | |
| CN112136965B (en) | Immunity-enhancing and growth-promoting fermented Chinese herbal medicine feed additive and preparation method thereof | |
| CN105132321A (en) | Enterococcus faecium, culture medium thereof for high-density solid-state fermentation, and high-density solid-state fermentation method | |
| CN103783321A (en) | Probiotic feed additive for Chinese softshell turtles and preparation method thereof | |
| CN103098980A (en) | Microorganism straw feed fermenting agent | |
| CN104605138A (en) | Poultry feed additive containing fermented Chinese yew, and preparation method and applications thereof | |
| CN104257826A (en) | Traditional Chinese medicine pycnonotus sinensis fermented granule for livestock and poultry and preparation method of traditional Chinese medicine pycnonotus sinensis fermented granule | |
| CN103829040B (en) | A kind of with the method for Chen Xiangcha and bacillus subtilis composite fermentation dregs of beans and application | |
| CN103960463B (en) | Ensiling tangerine dregs of rice feed and preparation technology thereof | |
| CN108771050A (en) | A kind of fish feed additive and preparation method thereof that palatability is high | |
| CN102334679A (en) | Method for preparing buckwheat pollen fermented health-care food | |
| CN108913627B (en) | A kind of leavening of aflatoxin degradation, preparation method and application | |
| CN106490307A (en) | A kind of method of antimicrobial composition fermentation Entermorpha | |
| KR20110069388A (en) | Method of preparing jujube probiotics using solid-state fermentation for livestocks and use of it | |
| CN106834164A (en) | For the Chinese yew solid medium and its application method of feed | |
| CN105505808A (en) | Complex micro-ecological preparation, additive, premix compound and batch mixture |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20170426 |