CN104257826A - Traditional Chinese medicine pycnonotus sinensis fermented granule for livestock and poultry and preparation method of traditional Chinese medicine pycnonotus sinensis fermented granule - Google Patents
Traditional Chinese medicine pycnonotus sinensis fermented granule for livestock and poultry and preparation method of traditional Chinese medicine pycnonotus sinensis fermented granule Download PDFInfo
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- CN104257826A CN104257826A CN201410492219.8A CN201410492219A CN104257826A CN 104257826 A CN104257826 A CN 104257826A CN 201410492219 A CN201410492219 A CN 201410492219A CN 104257826 A CN104257826 A CN 104257826A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/71—Ranunculaceae (Buttercup family), e.g. larkspur, hepatica, hydrastis, columbine or goldenseal
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/63—Oleaceae (Olive family), e.g. jasmine, lilac or ash tree
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/71—Ranunculaceae (Buttercup family), e.g. larkspur, hepatica, hydrastis, columbine or goldenseal
- A61K36/718—Coptis (goldthread)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/75—Rutaceae (Rue family)
- A61K36/756—Phellodendron, e.g. corktree
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/14—Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles
- A61K9/16—Agglomerates; Granulates; Microbeadlets ; Microspheres; Pellets; Solid products obtained by spray drying, spray freeze drying, spray congealing,(multiple) emulsion solvent evaporation or extraction
- A61K9/1605—Excipients; Inactive ingredients
- A61K9/1617—Organic compounds, e.g. phospholipids, fats
- A61K9/1623—Sugars or sugar alcohols, e.g. lactose; Derivatives thereof; Homeopathic globules
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/14—Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles
- A61K9/16—Agglomerates; Granulates; Microbeadlets ; Microspheres; Pellets; Solid products obtained by spray drying, spray freeze drying, spray congealing,(multiple) emulsion solvent evaporation or extraction
- A61K9/1605—Excipients; Inactive ingredients
- A61K9/1629—Organic macromolecular compounds
- A61K9/1652—Polysaccharides, e.g. alginate, cellulose derivatives; Cyclodextrin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/19—Preparation or pretreatment of starting material involving fermentation using yeast, bacteria or both; enzymatic treatment
Abstract
The invention belongs to the technical field of traditional Chinese medicine preparations for livestock and poultry and particularly relates to a traditional Chinese medicine pycnonotus sinensis fermented granule for livestock and poultry and a preparation method of the traditional Chinese medicine pycnonotus sinensis fermented granule. The pycnonotus sinensis fermented granule is prepared from the following traditional Chinese medicine raw materials in parts by weight: pycnonotus sinensis, rhizoma coptidis, phellodendron amurense and ash bark. The traditional Chinese medicine pycnonotus sinensis fermented granule for livestock and poultry disclosed by the invention can be used for preventing and treating diarrhea due to damp-heat and dysentery with pus and blood of the livestock and poultry, is convenient to use, small in toxic and side effect, green and free of residues.
Description
technical field
The invention belongs to poultry technical field of traditional Chinese medicine preparation, be specifically related to a kind of poultry Chinese medicine Radix Pulsatillae ferment particulate and preparation method thereof.
background technology
Along with developing rapidly of Chinese society economy and improving constantly of people's living standard, the consciousness of health of people life is also strengthening gradually.Therefore, the production of safe and high quality livestock products just seems particularly important.The a large amount of uses in feedstuff of antibiotic, chemical synthetic drug and hormone medicine bring very large harm to breeding production, have impact on food safety and people ' s health.Chinese herbal medicine, as the rarity of Chinese medicine, has the dual function of nourishing healthy and disease treatment.But Chinese herbal medicine also has many defects, as degree of absorbing difference, onset is slow, in recent years, the fermentation technique of Chinese herbal medicine earns widespread respect, and after research finds that Chinese herbal medicine undergoes microbial fermentation, its effective ingredient is fully extracted release, make it have higher biological activity and therapeutic value, and have features such as " safe green, noresidues ".The fermentation process of Chinese herbal medicine has solid fermentation and deep fermentation two kinds, the flowing of free water is not had in solid-state fermentation culture medium, limit the kind of microorganism, and there is gradient in nutrient concentrations, ferment uneven, the growth of thalline and to the absorption of nutrient substance and the juice of metabolite uneven.
The Radix Pulsatillae is loose to be made up of the Radix Pulsatillae, Rhizoma Coptidis, Cortex Phellodendri and Cortex Fraxini, has effect of heat-clearing and toxic substances removing and eliminating pathogenic heat from blood to cure dysentery, treatment poultry diarrhea due to damp-heat and dysentery pus and blood.The Radix Pulsatillae traditional using method of faling apart is that after being pulverized by Chinese medicine, directly spice is fed, and preparation process is simple, but due to drug particle size comparatively large, wherein there is again the active component being much difficult to stripping, cause the utilization rate of medicine low, drug effect can not be not fully exerted.
summary of the invention
The object of the present invention is to provide a kind of poultry Chinese medicine Radix Pulsatillae ferment particulate and preparation method thereof.
For achieving the above object, the present invention is by the following technical solutions:
A kind of poultry Chinese medicine Radix Pulsatillae ferment particulate, with parts by weight, raw material of Chinese medicine used is Radix Pulsatillae 5-25 part, Rhizoma Coptidis 20-40 part, Cortex Phellodendri 10-30 part, Cortex Fraxini 10-30 part, and mentioned component sum is 100 parts.
Prepare the method for poultry Chinese medicine Radix Pulsatillae ferment particulate, raw material of Chinese medicine is pulverized, adds MgSO
4fermentation medium is formed with distilled water; Inoculation fermentation strain after fermentation medium sterilizing, concussion is cultivated, and cultivates and terminates rear centrifugal supernatant, add adjuvant and make granule after supernatant concentration.
Fermented bacterium is one or more in lactobacillus (Lactobacillus), yeast (Yeast), bacillus subtilis (Bacillus subtilis), Cordyceps (Cordycepssinensis).
Concussion condition of culture is 100-200r/min, and cultivation temperature is 25-37 DEG C.
Concussion incubation time is 5-10d.
Adjuvant is one or more in glucose, maltodextrin, starch.
MgSO
4consumption be the 0.1-0.5% of raw material of Chinese medicine gross weight, the consumption of distilled water be the 3-5 of raw material of Chinese medicine gross weight doubly.
The present invention adopts deep fermentation method to carry out process of preparing Chinese medicine process to Chinese herbal medicine, overcome the defect of solid fermentation, sweat is easy to control, the raw material Radix Pulsatillae adopted, Rhizoma Coptidis, Cortex Phellodendri, Cortex Fraxini are after fermentable process, its fermentation liquid is prepared into granule, be convenient to the use of poultry, main component can have greatly changed, the macromolecular substances being wherein difficult to absorb becomes through the metabolism of microorganism the micromolecule easily absorbed, the content of toxicant reduces, and is conducive to playing its pharmacologic action better.Poultry of the present invention Chinese medicine Radix Pulsatillae ferment particulate can be used for diarrhea due to damp-heat and the dysentery pus and blood of preventing and treating poultry.
Herb fermenting technology is applied in the loose extracting solution of the Radix Pulsatillae by the present invention, modern biotechnology and traditional Chinese medicine processing methods combining are got up, the mode of fermentable is adopted to carry out scientific disposal to Chinese herbs decoction, the Radix Pulsatillae fermentation liquid obtained, carry out bed spray with adjuvant again after concentrated and prepare Radix Pulsatillae ferment particulate, the Radix Pulsatillae ferment particulate of gained has the effect of better heat-clearing and toxic substances removing and eliminating pathogenic heat from blood to cure dysentery, reduce again its toxic and side effects simultaneously, reduce the use amount of medicine, expand its clinical application effect, thus make up the defect that Chinese powder medicine onset is slow and bioavailability is low, easy to use, toxic and side effects is little, green noresidue.
detailed description of the invention
embodiment 1
The poultry raw material of Chinese medicine component of Chinese medicine Radix Pulsatillae ferment particulate: the Radix Pulsatillae 25 parts, Rhizoma Coptidis 40 parts, Cortex Phellodendri 20 parts, Cortex Fraxini 15 parts.
Preparation method: the composition of (1) fermentation medium: get after each Chinese medicine ingredients pulverized 20 orders in proportion, then add 0.1 part of MgSO
4fermentation medium is formed with 300 parts of distilled water; (2) above-mentioned fermentation medium is placed in 500ml triangular flask, in 121 DEG C of sterilizing 15min; (3) by lactobacillus inoculum to MRS solid medium, 37 DEG C cultivate 24h activation after, be inoculated in MRS fluid medium, be placed in 37 DEG C, 100r/min shaking table cultivate 18h obtain lactobacillus suspension; (4) pressed by lactobacillus suspension in the fermentation medium after 10wt% inoculum concentration access step (2) sterilizing, be placed in 37 DEG C, 180r/min shaking table carries out fermentation culture, fermentation time is 10 days; (5) after having fermented, by fermentation liquid again centrifuging and taking obtain supernatant; (6) carry out fluidized-bed spray granulation by mixing with glucose, starch after supernatant concentration, namely subpackage obtains poultry Chinese medicine Radix Pulsatillae ferment particulate again.
embodiment 2
The poultry raw material of Chinese medicine component of Chinese medicine Radix Pulsatillae ferment particulate: the Radix Pulsatillae 20 parts, Rhizoma Coptidis 35 parts, Cortex Phellodendri 20 parts, Cortex Fraxini 25 parts.
Preparation method: the composition of (1) fermentation medium: get after each Chinese medicine ingredients pulverized 20 orders in proportion, then add 0.2 part of MgSO
4fermentation medium is formed with 400 parts of distilled water; (2) above-mentioned fermentation medium is placed in 500ml triangular flask, in 121 DEG C of sterilizing 15min; (3) yeast is inoculated into PDA solid medium, 37 DEG C cultivate 24h activation after, be inoculated in PDA fluid medium, be placed in 37 DEG C, 100r/min shaking table cultivate 18h obtain yeast liquid; (4) by yeast liquid by 15 wt % inoculum concentrations access fermentation medium, be placed in 37 DEG C, 180r/min shaking table carries out fermentation culture, fermentation time is 8 days; (5) after having fermented, by fermentation liquid again centrifuging and taking obtain supernatant; (6) carry out fluidized-bed spray granulation by mixing with maltodextrin, starch after supernatant concentration, namely subpackage obtains poultry Chinese medicine Radix Pulsatillae ferment particulate again.
embodiment 3
The poultry raw material of Chinese medicine component of Chinese medicine Radix Pulsatillae ferment particulate: the Radix Pulsatillae 15 parts, Rhizoma Coptidis 30 parts, Cortex Phellodendri 30 parts, Cortex Fraxini 25 parts.
Preparation method: the composition of (1) fermentation medium: get after each Chinese medicine ingredients pulverized 20 orders in proportion, then add 0.5 part of MgSO
4fermentation medium is formed with 500 parts of distilled water; (2) above-mentioned fermentation medium is placed in 500ml triangular flask, in 121 DEG C of sterilizing 15min; (3) bacillus subtilis is inoculated into PDA solid medium, 37 DEG C cultivate 24h activation after, be inoculated in PDA fluid medium, be placed in 37 DEG C, 100r/min shaking table cultivate 18h obtain bacillus subtilis liquid; (4) by bacillus subtilis liquid by 15 wt % inoculum concentrations access fermentation medium, be placed in 37 DEG C, 180r/min shaking table carries out fermentation culture, fermentation time is 8 days; (5) after having fermented, by fermentation liquid again centrifuging and taking obtain supernatant; (6) carry out fluidized-bed spray granulation by mixing with glucose, maltodextrin after supernatant concentration, namely subpackage obtains poultry Chinese medicine Radix Pulsatillae ferment particulate again.
in Vitro Bacteriostasis effect measuring
Material: escherichia coli ATCC25922, staphylococcus aureus ATCC25923, nutrient agar, broth medium, the Radix Pulsatillae loose (lot number: 20131015, Henan Yawei Animal Medicine Co., Ltd.), poultry Chinese medicine Radix Pulsatillae ferment particulate (preparing by the embodiment of the present invention 1).
Method: get escherichia coli ATCC25922, staphylococcus aureus ATCC25923 is inoculated on plain agar flat board, cultivate 18h for 37 DEG C, the single colony inoculation of picking, in broth medium, is cultivated 16h for 37 DEG C, is got 0.1ml bacterium liquid and add 100ml sterilizing meat soup original bacteria liquid is diluted to 10
6cFU/ml.Empty for 12 sterilizings test tube is divided into 2 groups, often organize 6, first dilute bacterium liquid by all adding 1ml in test tube, Radix Pulsatillae aproll solution and each 1ml(0.4g/ml water of Radix Pulsatillae ferment particulate aqueous solution of the present invention is added again respectively) in first row test tube, getting 1ml after mixing adds in secondary series test tube, and doubling dilution is to the 5th row successively, discards 1ml, drug level is respectively 0.2g/ml, 0.1 g/ml, 0.05 g/ml, 0.025 g/ml, 0.0125 g/ml, and the 6th row in contrast.Test tube is sealed, is placed in 37 DEG C and cultivates 18h, observed result, as shown in table 1.
Table 1 test specimen is to the minimum In Vitro Bacteriostasis concentration (g/ml) of common bacteria
| Sample | Escherichia coli | Staphylococcus aureus |
| The Radix Pulsatillae falls apart | 0.1 | 0.2 |
| Poultry Chinese medicine Radix Pulsatillae ferment particulate | 0.05 | 0.025 |
As can be seen from Table 1, compared with poultry of the present invention are loose with the Radix Pulsatillae with Chinese medicine Radix Pulsatillae ferment particulate, need lower concentration can reach the effect suppressing escherichia coli and staphylococcus aureus, visible fermentable process can strengthen the drug effect of the Radix Pulsatillae, improves its In Vitro Bacteriostatic.
Claims (7)
1. a poultry Chinese medicine Radix Pulsatillae ferment particulate, is characterized in that: with parts by weight, and raw material of Chinese medicine used is Radix Pulsatillae 5-25 part, Rhizoma Coptidis 20-40 part, Cortex Phellodendri 10-30 part, Cortex Fraxini 10-30 part, and mentioned component sum is 100 parts.
2. prepare the method for poultry Chinese medicine Radix Pulsatillae ferment particulate as claimed in claim 1, it is characterized in that:
Raw material of Chinese medicine is pulverized, adds MgSO
4fermentation medium is formed with distilled water; Inoculation fermentation strain after fermentation medium sterilizing, concussion is cultivated, and cultivates and terminates rear centrifugal supernatant, add adjuvant and make granule after supernatant concentration.
3. prepare as claimed in claim 2 the method for poultry Chinese medicine Radix Pulsatillae ferment particulate, it is characterized in that: fermented bacterium is one or more in lactobacillus, yeast, bacillus subtilis, Cordyceps.
4. prepare the method for poultry Chinese medicine Radix Pulsatillae ferment particulate as claimed in claim 2 or claim 3, it is characterized in that: concussion condition of culture is 100-200r/min, cultivation temperature is 25-37 DEG C.
5. prepare the method for poultry Chinese medicine Radix Pulsatillae ferment particulate as claimed in claim 2 or claim 3, it is characterized in that: concussion incubation time is 5-10d.
6. prepare as claimed in claim 2 or claim 3 the method for poultry Chinese medicine Radix Pulsatillae ferment particulate, it is characterized in that: adjuvant is one or more in glucose, maltodextrin, starch.
7. prepare the method for poultry Chinese medicine Radix Pulsatillae ferment particulate as claimed in claim 2 or claim 3, it is characterized in that: MgSO
4consumption be the 0.1-0.5% of raw material of Chinese medicine gross weight, the consumption of distilled water be the 3-5 of raw material of Chinese medicine gross weight doubly.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105664091A (en) * | 2016-01-21 | 2016-06-15 | 河南金尔康生物科技有限公司 | Traditional Chinese veterinary medicine composition with efficacies of spleen and stomach tonifying, diuresis inducing and edema alleviating, fermentation preparation and application thereof |
| CN108607013A (en) * | 2016-12-13 | 2018-10-02 | 河南后羿实业集团有限公司 | A kind of fermented type Chinese bulbul oral solution and preparation method thereof |
| CN108686021A (en) * | 2018-08-15 | 2018-10-23 | 福建傲农生物科技集团股份有限公司 | A kind of plant fermentation object and preparation method thereof of prevention diarrhea of pigs |
| CN111034857A (en) * | 2020-01-06 | 2020-04-21 | 唐人神集团股份有限公司 | Traditional Chinese medicine additive for preventing diarrhea, preparation method thereof and livestock feed for preventing diarrhea |
| CN114196592A (en) * | 2021-12-31 | 2022-03-18 | 河南农业大学 | Lactobacillus casei R and fermentation method and application thereof |
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| CN111034857A (en) * | 2020-01-06 | 2020-04-21 | 唐人神集团股份有限公司 | Traditional Chinese medicine additive for preventing diarrhea, preparation method thereof and livestock feed for preventing diarrhea |
| CN114196592A (en) * | 2021-12-31 | 2022-03-18 | 河南农业大学 | Lactobacillus casei R and fermentation method and application thereof |
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| CN104257826B (en) | 2017-07-04 |
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