CN104257826B - A kind of livestock and poultry Chinese medicine Chinese bulbul ferment particulate and preparation method thereof - Google Patents

A kind of livestock and poultry Chinese medicine Chinese bulbul ferment particulate and preparation method thereof Download PDF

Info

Publication number
CN104257826B
CN104257826B CN201410492219.8A CN201410492219A CN104257826B CN 104257826 B CN104257826 B CN 104257826B CN 201410492219 A CN201410492219 A CN 201410492219A CN 104257826 B CN104257826 B CN 104257826B
Authority
CN
China
Prior art keywords
chinese medicine
parts
chinese
livestock
poultry
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN201410492219.8A
Other languages
Chinese (zh)
Other versions
CN104257826A (en
Inventor
赖登明
张遂平
郭芳茹
杨慧敏
吴胜耀
刘方舟
王书丽
杨果
闫树森
牛咏梅
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
HENAN YAWEI ANIMAL MEDICINE CO Ltd
Original Assignee
HENAN YAWEI ANIMAL MEDICINE CO Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by HENAN YAWEI ANIMAL MEDICINE CO Ltd filed Critical HENAN YAWEI ANIMAL MEDICINE CO Ltd
Priority to CN201410492219.8A priority Critical patent/CN104257826B/en
Publication of CN104257826A publication Critical patent/CN104257826A/en
Application granted granted Critical
Publication of CN104257826B publication Critical patent/CN104257826B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/71—Ranunculaceae (Buttercup family), e.g. larkspur, hepatica, hydrastis, columbine or goldenseal
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/63—Oleaceae (Olive family), e.g. jasmine, lilac or ash tree
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/71—Ranunculaceae (Buttercup family), e.g. larkspur, hepatica, hydrastis, columbine or goldenseal
    • A61K36/718—Coptis (goldthread)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/75—Rutaceae (Rue family)
    • A61K36/756—Phellodendron, e.g. corktree
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/14—Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles
    • A61K9/16—Agglomerates; Granulates; Microbeadlets ; Microspheres; Pellets; Solid products obtained by spray drying, spray freeze drying, spray congealing,(multiple) emulsion solvent evaporation or extraction
    • A61K9/1605—Excipients; Inactive ingredients
    • A61K9/1617—Organic compounds, e.g. phospholipids, fats
    • A61K9/1623—Sugars or sugar alcohols, e.g. lactose; Derivatives thereof; Homeopathic globules
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/14—Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles
    • A61K9/16—Agglomerates; Granulates; Microbeadlets ; Microspheres; Pellets; Solid products obtained by spray drying, spray freeze drying, spray congealing,(multiple) emulsion solvent evaporation or extraction
    • A61K9/1605—Excipients; Inactive ingredients
    • A61K9/1629—Organic macromolecular compounds
    • A61K9/1652—Polysaccharides, e.g. alginate, cellulose derivatives; Cyclodextrin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/10—Preparation or pretreatment of starting material
    • A61K2236/19—Preparation or pretreatment of starting material involving fermentation using yeast, bacteria or both; enzymatic treatment

Landscapes

  • Health & Medical Sciences (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Alternative & Traditional Medicine (AREA)
  • Biotechnology (AREA)
  • Botany (AREA)
  • Medical Informatics (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Medicines Containing Plant Substances (AREA)

Abstract

The invention belongs to livestock and poultry technical field of traditional Chinese medicine preparation, and in particular to a kind of livestock and poultry Chinese medicine Chinese bulbul ferment particulate and preparation method thereof.In parts by weight, Chinese medicine material used is the Chinese bulbul, the coptis, golden cypress and the bark of ash to the Chinese bulbul ferment particulate.Livestock and poultry of the invention Chinese medicine Chinese bulbul ferment particulate can be used to prevent and treat the diarrhea due to damp-heat and diarrhea purulence blood of livestock and poultry, and easy to use, toxic and side effect is small, green noresidue.

Description

A kind of livestock and poultry Chinese medicine Chinese bulbul ferment particulate and preparation method thereof
Technical field
The invention belongs to livestock and poultry technical field of traditional Chinese medicine preparation, and in particular to a kind of livestock and poultry Chinese medicine Chinese bulbul ferment particulate And preparation method thereof.
Background technology
With the continuous improvement developed rapidly with people's living standard of Chinese society economy, the consciousness of health of people life Also gradually strengthening.Therefore, the production of safe and high quality livestock products is just particularly important.Antibiotic, chemical synthetic drug and swash Plain class medicine is a large amount of using very big harm is brought to breeding production in feed, have impact on food security and people's health. Chinese herbal medicine as Chinese medicine rarity, the double action with nutrition and health care and disease treatment.But Chinese herbal medicine also has many lacking Fall into, such as degree of absorbing is poor, work slow etc., in recent years, the fermentation technique of Chinese herbal medicine earns widespread respect, and research finds Chinese herbal medicine After undergoing microbial fermentation, its active ingredient is fully extracted release, makes it have bioactivity and therapeutic value higher, and There is features such as " safe green, noresidues ".The fermentation process of Chinese herbal medicine has two kinds of solid state fermentation and deep fermentation, solid-state hair There is no the flowing of free water in ferment culture medium, limit the species of microorganism, and there is gradient in nutrient concentrations, fermentation is uneven Even, the juice of the growth of thalline and the absorption to nutriment and metabolite is uneven.
Chinese bulbul is made up of the Chinese bulbul, the coptis, golden cypress and the bark of ash, has functions that clearing heat and detoxicating and removes heat from the blood and relieve diarrhea, and controls Treat livestock and poultry diarrhea due to damp-heat and diarrhea purulence blood.Chinese bulbul tradition application method is direct spice feeding after Chinese medicine is crushed, and is prepared Process is simple, but because drug particle size is larger, wherein there is the active component for being much difficult to dissolution again, causes the utilization rate of medicine Low, drug effect can not be not fully exerted.
The content of the invention
It is an object of the invention to provide a kind of livestock and poultry Chinese medicine Chinese bulbul ferment particulate and preparation method thereof.
To achieve the above object, the present invention uses following technical scheme：
A kind of livestock and poultry Chinese medicine Chinese bulbul ferment particulate, in parts by weight, Chinese medicine material used is Chinese bulbul 5-25 parts, Huang Even 20-40 parts, golden cypress 10-30 parts, bark of ash 10-30 parts, mentioned component sum is 100 parts.
The livestock and poultry method of Chinese medicine Chinese bulbul ferment particulate is prepared, Chinese medicine material is crushed, add MgSO4With distilled water shape Into fermentation medium；Fermented bacterium is inoculated with after fermentation medium sterilizing, concussion and cultivate, culture is centrifuged to obtain supernatant after terminating, on Auxiliary material is added to be made particle after clear liquid concentration.
Fermented bacterium is lactic acid bacteria（Lactobacillus）, saccharomycete（Yeast）, bacillus subtilis（Bacillus subtilis）, Cordyceps Militaris（Cordycepssinensis）In one or more.
Concussion and cultivate condition is 100-200r/min, and cultivation temperature is 25-37 DEG C.
The concussion and cultivate time is 5-10d.
Auxiliary material is one or more in glucose, maltodextrin, starch.
MgSO4Consumption be the 0.1-0.5% of Chinese medicine material gross weight, the consumption of distilled water is Chinese medicine material gross weight 3-5 times.
The present invention carries out processing treatment using deep fermentation method to Chinese herbal medicine, overcomes the defect of solid state fermentation, sends out Ferment process is easily controllable, after the raw material Chinese bulbul, the coptis, golden cypress, the bark of ash for being used are through microbial fermentation treatment, by its zymotic fluid Particle is prepared into, is easy to the use of livestock and poultry, main component can have greatly changed, wherein being difficult to the macromolecular complex for absorbing Matter becomes the small molecule of easy absorption by the metabolism of microorganism, and the content reduction of toxicant is conducive to preferably playing Its pharmacologic action.Livestock and poultry of the invention Chinese medicine Chinese bulbul ferment particulate can be used to prevent and treat the diarrhea due to damp-heat and diarrhea purulence of livestock and poultry Blood.
The present invention herb fermenting technology is applied in the extract solution of Chinese bulbul, modern biotechnology and it is traditional in Medicine concocting method combines, and scientific disposal is carried out to Chinese herbs decoction by the way of microbial fermentation, the Chinese bulbul hair for obtaining Zymotic fluid, carries out bed spray and prepares Chinese bulbul ferment particulate with auxiliary material again after concentration, and the Chinese bulbul ferment particulate of gained has Effect that is preferably clearing heat and detoxicating and removing heat from the blood and relieve diarrhea, while reducing its toxic and side effect again, reduces the usage amount of medicine, expands it and faces Bed application effect, works the slow and low defect of bioavilability so as to make up traditional Chinese powder medicine, and easy to use, toxic and side effect is small, green Color noresidue.
Specific embodiment
Embodiment 1
The livestock and poultry Chinese medicine material component of Chinese medicine Chinese bulbul ferment particulate：25 parts of the Chinese bulbul, 40 parts of the coptis, 20 parts of golden cypress, 15 parts of the bark of ash.
Preparation method：（1）The composition of fermentation medium：Take in proportion after each traditional Chinese medicine ingredients crushed 20 mesh, add 0.1 Part MgSO4Fermentation medium is formed with 300 parts of distilled water；（2）Above-mentioned fermentation medium is placed in 500ml triangular flasks, in 121 DEG C sterilizing 15min；（3）By lactobacillus inoculum to MRS solid mediums, after 37 DEG C of culture 24h activation, the training of MRS liquid is inoculated in Support base in, be placed in 37 DEG C, 100r/min shaking table cultures 18h obtain lactobacillus suspension；（4）Lactobacillus suspension is connect by 10wt% inoculum concentrations Enter step（2）In fermentation medium after sterilizing, be placed in 37 DEG C, 180r/min shaking tables carry out fermented and cultured, fermentation time is 10 My god；（5）After the completion of fermentation, by zymotic fluid, centrifuging and taking obtains supernatant again；（6）To be mixed with glucose, starch again after supernatant concentration Conjunction carries out fluidized-bed spray granulation, and packing obtains final product livestock and poultry Chinese medicine Chinese bulbul ferment particulate.
Embodiment 2
The livestock and poultry Chinese medicine material component of Chinese medicine Chinese bulbul ferment particulate：20 parts of the Chinese bulbul, 35 parts of the coptis, 20 parts of golden cypress, 25 parts of the bark of ash.
Preparation method：（1）The composition of fermentation medium：Take in proportion after each traditional Chinese medicine ingredients crushed 20 mesh, add 0.2 Part MgSO4Fermentation medium is formed with 400 parts of distilled water；（2）Above-mentioned fermentation medium is placed in 500ml triangular flasks, in 121 DEG C sterilizing 15min；（3）Saccharomycete is inoculated into PDA solid mediums, after 37 DEG C of culture 24h activation, the training of PDA liquid is inoculated in Support base in, be placed in 37 DEG C, 100r/min shaking table cultures 18h obtain yeast liquid；（4）Yeast liquid is pressed into 15 wt % inoculum concentrations In accessing fermentation medium, be placed in 37 DEG C, 180r/min shaking tables carry out fermented and cultured, fermentation time is 8 days；（5）Fermentation is completed Afterwards, by zymotic fluid, centrifuging and taking obtains supernatant again；（6）Fluid bed is carried out by mixing with maltodextrin, starch again after supernatant concentration Spraying granulation, packing obtains final product livestock and poultry Chinese medicine Chinese bulbul ferment particulate.
Embodiment 3
The livestock and poultry Chinese medicine material component of Chinese medicine Chinese bulbul ferment particulate：15 parts of the Chinese bulbul, 30 parts of the coptis, 30 parts of golden cypress, 25 parts of the bark of ash.
Preparation method：（1）The composition of fermentation medium：Take in proportion after each traditional Chinese medicine ingredients crushed 20 mesh, add 0.5 Part MgSO4Fermentation medium is formed with 500 parts of distilled water；（2）Above-mentioned fermentation medium is placed in 500ml triangular flasks, in 121 DEG C sterilizing 15min；（3）Bacillus subtilis is inoculated into PDA solid mediums, after 37 DEG C of culture 24h activation, PDA is inoculated in In fluid nutrient medium, be placed in 37 DEG C, 100r/min shaking table cultures 18h obtain bacillus subtilis bacterium solution；（4）By bacillus subtilis Liquid by 15 wt % inoculum concentrations access fermentation medium in, be placed in 37 DEG C, 180r/min shaking tables carry out fermented and cultured, fermentation time It is 8 days；（5）After the completion of fermentation, by zymotic fluid, centrifuging and taking obtains supernatant again；（6）By after supernatant concentration again with glucose, malt Dextrin mixing carries out fluidized-bed spray granulation, and packing obtains final product livestock and poultry Chinese medicine Chinese bulbul ferment particulate.
In Vitro Bacteriostasis effect measuring
Material：Escherichia coli ATCC25922, staphylococcus aureus ATCC25923, nutrient agar, common meat Soup culture medium, Chinese bulbul（Lot number：20131015, Henan Yawei Animal Medicine Co., Ltd.）, livestock and poultry with the Chinese medicine Chinese bulbul send out Ferment particle（Prepared by the embodiment of the present invention 1）.
Method：Escherichia coli ATCC25922, staphylococcus aureus ATCC25923 is taken to be inoculated on plain agar flat board, 37 DEG C of culture 18h, picking single bacterium colony is inoculated in broth medium, and 37 DEG C of culture 16h take 0.1ml bacterium solutions plus 100ml goes out Original bacteria liquid is diluted to 10 by bacterial context soup6CFU/ml.12 empty test tubes of sterilizing are divided into 2 groups, every group 6, first will be in test tube all 1ml dilution bacterium solutions are added, then the Chinese bulbul aqueous solution and the Chinese bulbul of the invention fermentation are separately added into first row test tube Each 1ml of the grain aqueous solution（0.4g/ml water）, taking after mixing in 1ml addition secondary series test tubes, doubling dilution is abandoned to the 5th row successively 1ml, drug concentration is gone to be respectively 0.2g/ml, 0.1 g/ml, 0.05 g/ml, 0.025 g/ml, 0.0125 g/ml, the 6th row As control.Test tube is sealed, 37 DEG C of culture 18h are placed in, result is observed, as shown in table 1.
Minimum In Vitro Bacteriostasis concentration of the test specimen of table 1 to common bacteria（g/ml）
Sample Escherichia coli Staphylococcus aureus
Chinese bulbul 0.1 0.2
Livestock and poultry Chinese medicine Chinese bulbul ferment particulate 0.05 0.025
As can be seen from Table 1, livestock and poultry of the present invention Chinese medicine Chinese bulbul ferment particulate compared with Chinese bulbul, it is necessary to relatively low Concentration is that can reach the effect for suppressing Escherichia coli and staphylococcus aureus, it is seen that microbial fermentation treatment can strengthen hoary hair The drug effect of father-in-law, improves its In Vitro Bacteriostatic.

Claims (1)

1. a kind of method for preparing livestock and poultry Chinese medicine Chinese bulbul ferment particulate, it is characterised in that：In parts by weight, Chinese medicine used is former Expect to be 25 parts of the Chinese bulbul, 40 parts of the coptis, 20 parts of golden cypress, 15 parts of the bark of ash；
Following steps are used during preparation：（1）The composition of fermentation medium：Take in proportion after each traditional Chinese medicine ingredients crushed 20 mesh, then Add 0.1 part of MgSO4Fermentation medium is formed with 300 parts of distilled water；（2）Above-mentioned fermentation medium is placed in 500ml triangular flasks In, sterilize 15min in 121 DEG C；（3）By lactobacillus inoculum to MRS solid mediums, after 37 DEG C of culture 24h activation, it is inoculated in In MRS fluid nutrient mediums, be placed in 37 DEG C, 100r/min shaking table cultures 18h obtain lactobacillus suspension；（4）Lactobacillus suspension is pressed into 10wt% Inoculum concentration accesses step（2）In fermentation medium after sterilizing, be placed in 37 DEG C, 180r/min shaking tables carry out fermented and cultured, ferment Time is 10 days；（5）After the completion of fermentation, by zymotic fluid, centrifuging and taking obtains supernatant again；（6）By after supernatant concentration again with grape Sugar, starch mixing carry out fluidized-bed spray granulation, and packing obtains final product livestock and poultry Chinese medicine Chinese bulbul ferment particulate.
CN201410492219.8A 2014-09-24 2014-09-24 A kind of livestock and poultry Chinese medicine Chinese bulbul ferment particulate and preparation method thereof Active CN104257826B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201410492219.8A CN104257826B (en) 2014-09-24 2014-09-24 A kind of livestock and poultry Chinese medicine Chinese bulbul ferment particulate and preparation method thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201410492219.8A CN104257826B (en) 2014-09-24 2014-09-24 A kind of livestock and poultry Chinese medicine Chinese bulbul ferment particulate and preparation method thereof

Publications (2)

Publication Number Publication Date
CN104257826A CN104257826A (en) 2015-01-07
CN104257826B true CN104257826B (en) 2017-07-04

Family

ID=52149483

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201410492219.8A Active CN104257826B (en) 2014-09-24 2014-09-24 A kind of livestock and poultry Chinese medicine Chinese bulbul ferment particulate and preparation method thereof

Country Status (1)

Country Link
CN (1) CN104257826B (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105664091B (en) * 2016-01-21 2019-03-26 河南金尔康生物科技有限公司 A kind of spleen-and-stomach-invigorating, the veterinary Chinese medicinal composition of inducing diuresis for removing edema, fermentation preparation and its application
CN108607013A (en) * 2016-12-13 2018-10-02 河南后羿实业集团有限公司 A kind of fermented type Chinese bulbul oral solution and preparation method thereof
CN108686021A (en) * 2018-08-15 2018-10-23 福建傲农生物科技集团股份有限公司 A kind of plant fermentation object and preparation method thereof of prevention diarrhea of pigs
CN111034857A (en) * 2020-01-06 2020-04-21 唐人神集团股份有限公司 Traditional Chinese medicine additive for preventing diarrhea, preparation method thereof and livestock feed for preventing diarrhea
CN114196592A (en) * 2021-12-31 2022-03-18 河南农业大学 Lactobacillus casei R and fermentation method and application thereof

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1939467A (en) * 2006-09-28 2007-04-04 天津生机集团有限公司 Chinese medicine for preventing and treating fowl colibacillosis
CN1939466A (en) * 2006-09-28 2007-04-04 天津生机集团有限公司 Chinese-medicinal composition for treating domestic animal and fowl's enteritis
CN101491548B (en) * 2008-12-26 2013-05-29 天津瑞普生物技术股份有限公司 Suspension suitable for animal directly-drinking and preparation method thereof
CN101664421B (en) * 2009-09-30 2011-05-25 北京大北农科技集团股份有限公司 Traditional Chinese biological preparation for preventing and treating livestock diarrhea and preparation method and feedstuff thereof
CN101810716A (en) * 2009-12-17 2010-08-25 河南省康星药业有限公司 Preparation method of Chinese pulsatilla root submicron powder at normal temperature
CN102114113B (en) * 2010-01-05 2012-09-12 四川滇虹医药开发有限公司 Preparation method of Chinese pulsatilla root decoction granules
CN102283927A (en) * 2010-06-17 2011-12-21 苏州知微堂生物科技有限公司 Technology for preparing novel integrated dosage form of windflower decoction
CN102988552A (en) * 2012-11-02 2013-03-27 青岛康地恩药业股份有限公司 Traditional Chinese medicine compound radix pulsatillae particle for treating chicken colibacillosis and preparation method and application thereof
CN103830398A (en) * 2012-11-22 2014-06-04 青岛康地恩动物药业有限公司 Composite Chinese pulsatilla root granular preparation, and preparation method thereof

Also Published As

Publication number Publication date
CN104257826A (en) 2015-01-07

Similar Documents

Publication Publication Date Title
CN104257826B (en) A kind of livestock and poultry Chinese medicine Chinese bulbul ferment particulate and preparation method thereof
CN107446852B (en) One lactobacillus plantarum and its application in terms of fermented feed
CN104404092B (en) A kind of biological concentration method of conjugated linoleic acid isomers
CN106754619A (en) It is a kind of that the method that traditional Chinese medicinal components promote growth of probiotics is added in grain culture medium
CN105685475A (en) Method for preparing feed antibiotic substitute from subtilin producing bacillus subtilis fermented Chinese herbal medicine
CN108186784B (en) Dandelion composite enzyme and preparation method and application thereof
CN107217020B (en) Culture medium suitable for lactobacillus acidophilus and preparation method thereof
CN102703342B (en) Bacillus velezensis ZJ20 strain and liquid preparations thereof
CN103289937B (en) Method for producing bacillus laterosporus live bacteria by high density solid fermentation
JP2021503919A (en) Flavobacterium brave biocontrol strain that efficiently degrades aflatoxin and its use
CN103451144B (en) Microbial growth promoters, Preparation Method And The Use
CN107223956A (en) Antrodia camphorata ferment and preparation method thereof
CN112136965B (en) Immunity-enhancing and growth-promoting fermented Chinese herbal medicine feed additive and preparation method thereof
CN105886430A (en) Method for producing bacillus amyloliquefaciens bacterial agent through solid-state fermentation
CN114032190A (en) Lactobacillus reuteri capable of fermenting dendrobium and effectively repairing solar dermatitis by fermentation liquor of dendrobium
CN102823718A (en) Method for preparing probiotics through liquid and solid double fermentation
CN102367417B (en) Traditional Chinese medicine probiotics liquid medium used for fish feed, culturing method and feed additive
CN107048364A (en) A kind of mulberry leaf powder rich in gamma aminobutyric acid and preparation method thereof
CN104030851A (en) Lentinula edodes culture medium in which shaddock peel is added
CN107279973A (en) Honeysuckle ferment and preparation method thereof
CN110574926A (en) process for high-value utilization of innominate sunflower
CN102851334B (en) Fermentation medium and fermentation method of aflatoxin B1
CN105647808A (en) Method for preserving Lactobacillus bulgaricus with agaro-oligosaccharide used as protective agent
CN108707569A (en) Enterococcus faecium high-efficiency fermenting culture medium and its fermentation culture method
CN104604503B (en) A kind of Biological control anti-root of Rhodiola sachalinensis maize ear rot and the method for growth-promoting

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant