CN104403973B - It is a kind of that there is the digestion lactobacillus for removing patulin effect and its application - Google Patents
It is a kind of that there is the digestion lactobacillus for removing patulin effect and its application Download PDFInfo
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- CN104403973B CN104403973B CN201410734478.7A CN201410734478A CN104403973B CN 104403973 B CN104403973 B CN 104403973B CN 201410734478 A CN201410734478 A CN 201410734478A CN 104403973 B CN104403973 B CN 104403973B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/225—Lactobacillus
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
- A23L5/20—Removal of unwanted matter, e.g. deodorisation or detoxification
- A23L5/28—Removal of unwanted matter, e.g. deodorisation or detoxification using microorganisms
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/34—Nature of the water, waste water, sewage or sludge to be treated from industrial activities not provided for in groups C02F2103/12 - C02F2103/32
Abstract
There is the digestion lactobacillus for removing patulin effect and its application the invention discloses a kind of, belong to microbial technology field.Digestion lactobacillus (Lactobacillus alimentris) provided by the present invention has acid resistance, patulin that can be in the fast eliminating aqueous solution, is 1 × 10 by thalline number10CFU/mL digestion lactobacillus co-cultures with the patulin that concentration is 1000 μ g/L, co-cultures 4h, can reach more than 80% to the clearance rate of patulin, after co-culturing 8h, more than 90% is can reach to its clearance rate.The patulin in cider and mouldy pomace can be significantly reduced simultaneously.Described digestion lactobacillus is used to remove the patulin in food or feed, has very extensive application prospect.
Description
Technical field
There is the digestion lactobacillus for removing patulin effect and its application the present invention relates to a kind of, belong to microbial technique
Field.
Background technology
Patulin is also known as clavacin, is a kind of poisonous caused by the mould metabolism such as Penicillium, aspergillus, the mould category of silk clothes
Fungus metabolite.It has extensive and strong toxic action to people and animal.Acute toxicity show as twitch, spasm,
Hypodermis oedema, lung enteremia, enteritis, anuria are until dead；Chronic toxicity can cause gastric ulcer, and be immunized with suppression,
Teratogenesis and potential carcinogenicity etc..Patulin can be dissolved in water, ethanol, acetone, ethyl acetate and chloroform, be slightly soluble in second
Ether, benzene, insoluble in petroleum ether, chemical property is stable in acid condition.
Patulin is a kind of worldwide pollutant, and the pollution of patulin is quite general in food and Feed Manufacturing
Time, be widely present in the products such as apple, grape, orange, pineapple, corn, cheese and animal feed, particularly fruit and with
Fruit is that the patulin pollution in the product to process raw material is the most serious.Patulin is mainly by penicillium expansum in fruit
Caused by (Penicillium expansum), this germ can not only cause fruit rot, can also be secreted into fruit tissue
Substantial amounts of patulin.In fruit juice production industry, if rotten fruit is mixed into raw material, then the fruit juice of output will
There is the residual of different degrees of patulin, the health to consumer (particularly children) brings serious harm.It is based on
Patulin is to the potential hazard of health, and much country all does to the maximum limitation of patulin in fruit juice product in the world
Regulation, EU, USFDA provides that maximum limit the quantity of the patulin in fruit juice product is 50 μ g/kg, maximum limit in infant's product
Measure and suggest that patulin intake is no more than 0.4 μ g/kg body weight for each person every day for 10 μ g/kg, WHO.
Mainly entered at present by the chemical means such as the physical means such as absorption, microwave treatment or addition additive, ozone processing
Row removes patulin, but physical method effect is not very good, and is easy to make patulin gather sorbing material
In, the potential threat to environment structure.And the problems such as high cost, security and cutting mechanisms are still not clear be present in chemical method,
It yet there are no and be applied in produce reality.
In view of traditional method has various problems, need badly and research and develop new patulin removal means.
It is universal not high that the efficiency of patulin is removed by microorganism at present, and is confined to remove in the aqueous solution or nutrient solution
Patulin, be seldom applied in food and feed.It is contemplated that filter out the microorganism that energy high-efficient cleaning removes patulin
And use it for removing the patulin in food and feed.
The content of the invention
It is an object of the invention to provide one kind digestion lactobacillus (Lactobacillus alimentris), the bacterial strain in
It is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center within 09 15th, 2014, preservation address is Beijing
No. 3 Institute of Microorganism, Academia Sinica of institute of city Chaoyang District North Star West Road 1, deposit number are CGMCC No.9663.
The digestion lactobacillus has following characteristic：
(1) colony characteristicses：The well-grown on MRS culture mediums, form milky, translucent, circular colonies, bacterium colony surface
Moistening.Ne ar is in middle elongated rod shape, and both ends are oval, and Gram's staining reaction is positive, without motion, does not produce bud and embraces.
(2) growth characteristics：The minimum growth temperature of the bacterial strain is 15 DEG C, and maximum growth temperature is 40 DEG C, and the bacterial strain is with 30-
37 DEG C of growth temperature is preferred, and highest and minimum initial growth pH are 9.0 and 3.0, and the initial pH of the most suitable growth is 6.0；The digestion
The period of delay of lactobacillus is relatively short, and 4h or so initially enters exponential phase 12h and just reaches stationary phase.
(3) there is acid resistance, the well-grown under pH3.0-9.0 environmental conditions, survived under the environment of pH 2.5 good.
(4) in the aqueous solution containing 1000 μ g/L patulins, patulin concentration can be dropped to 11.1 μ g/L.
(5), can be to the clearance rate of patulin up to more than 90% in the culture medium containing 1000 μ g/L patulins.
(6), can be by the content of patulin from 966.95 μ g/L in the cider containing 966.95 μ g/L patulins
Drop to 54.41 μ g/L, clearance rate is up to 94.37%.
The present invention also provides the biology system of digestion lactobacillus CGMCC No.9663 prepared for removing patulin a kind of
Agent, it is that centrifugation after digestion lactobacillus CGMCC No.9663 activation cultures is obtained into digestion lactobacillus thalline, thalline is collected after washing
Obtain digesting lactobacillus biological agent.Or the thalline for obtaining collection is resuspended with protective agent reaches concentration 10 again10CFU/mL,
Suspension culture is freeze-dried afterwards for a period of time to obtain digestion lactobacillus bacterium powder biological agent.
Described protective agent can be skimmed milk power, trehalose or sucrose etc..
The digestion lactobacillus CGMCC No.9663 bacterium have following purposes：
(1) biological agent for removing patulin is made；
(2) it is used for removing the patulin in the aqueous solution, fruit juice and food and feed.
The digestion lactobacillus CGMCC No.9663 of the present invention have acid resistance, and good tolerance is known as to Penicillium patulum,
It is resistant to the patulin solution that initial concentration is 10000 μ g/L.And stronger scavenging action is known as to Penicillium patulum, can
Significantly reduce the content of patulin in the aqueous solution, cider and mouldy pomace.Described digestion lactobacillus CGMCC
No.9663 is used to remove the patulin in food or feed, has very extensive application prospect.
Biomaterial preservation
Lactobacillus (Lactobacillus alimentris) is digested, has been preserved in the micro- life of China on 09 15th, 2014
Thing culture presevation administration committee common micro-organisms center, preservation address are Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3 China
Institute of microbiology of the academy of sciences, deposit number are CGMCC No.9663.
Brief description of the drawings
Fig. 1 is patulin to digestion lactobacillus CGMCC No.9663 growth effects analysis.
Fig. 2 is that different time digests elimination efficiency figures of the lactobacillus CGMCC No.9663 to patulin.
After Fig. 3 is addition digestion lactobacillus CGMCC No.9663 in cider patulin content variation diagram.
Fig. 4 is the content of addition digestion lactobacillus CGMCC No.9663 mouldy pomace and patulin in control group
Figure.
Embodiment
Experimental method described in following embodiments, it is conventional method unless otherwise specified；The reagent and biological material
Material, unless otherwise specified, is commercially obtained.
The preparation of the patulin standard items mother liquor of embodiment 1
The patulin is patulin standard items, is purchased from Beijing Tai Leqi Science and Technology Ltd.s, is configured to during use
100mg/L mother liquor.Collocation method is：The accurate patulin standard items for weighing 5mg, are dissolved in 50mL ethyl acetate, fully
Dissolving is mixed, and -20 DEG C are stored in after carrying out filtration sterilization using 0.22 μm of miillpore filter.
Embodiment 2 prepares the biological agent of the digestion lactobacillus CGMCC No.9663 for removing patulin
Digestion lactobacillus CGMCC No.9663 are inoculated in MRS culture mediums with 2% inoculum concentration, are 30~37 DEG C in temperature,
After cultivating 18-24h in the shaking table that shaking speed is 150r/min-200r/min, through 5000r/min, 10min centrifugations are digested
Lactobacillus thalline, the thalline after centrifugation is cleaned into three times centrifugation afterwards with 0.85% physiological saline and obtains digesting lactobacillus CGMCC
No.9663 biological agents.
Or thalline is resuspended with protective agent again and reaches concentration 1010CFU/mL.Described protective agent is 100g/L defatted milks
Powder, 100g/L trehaloses and 150g/L sucrose.Then, suspension preculture 60min under conditions of 37 DEG C of temperature is allowed, then
In -20 DEG C of pre-freeze 2-4h, finally it is freeze-dried to obtain digestion lactobacillus CGMCC No.9663 bacterium powder biological agents.
Influence of the patulin of embodiment 3 to digestion lactobacillus CGMCC No.9663 growths
1st, the preparation of the MRS nutrient solutions of the patulin containing various concentrations
As needed, the patulin standard items mother liquor that concentration in certain volume embodiment 1 is 100mg/L is accurately measured,
MRS nutrient solutions are dissolved in after being dried up with nitrogen, and are settled to 100mL.Such as the patulin nutrient solution that compound concentration is 1000 μ g/L
When, the patulin standard items 1mL that concentration is 100mg/L is accurately measured first, is dissolved in after being dried up with nitrogen in MRS nutrient solutions,
100mL is settled to, that is, obtains the patulin nutrient solution that concentration is 1000 μ g/L.
2nd, patulin is to digestion lactobacillus CGMCC No.9663 growth effects analysis
Digestion lactobacillus CGMCC No.9663 through overactivation are inoculated into the difference that step 1 prepares by 2% inoculum concentration
In patulin MRS nutrient solutions containing 0 μ g/L, 2000 μ g/L, 5000 μ g/L and 10000 μ g/L, 96 holes are sub-packed in after inoculation
Plate, temperature is placed in be cultivated in 37 DEG C of incubators, each handle 4 Duplicate Samples, while blank group is set.Total co-cultivation 24 is small
When, take out 96 orifice plates every two hours and use the OD values under ELIASA survey 600nm.
As a result such as Fig. 1, it can be seen that digestion lactobacillus CGMCC No.9663 give birth in the culture medium of various concentrations
Long curve essentially coincides with blank group, illustrates that digest lactobacillus CGMCC No.9663 has stronger tolerance energy to patulin
Power.
Embodiment 4 digests lactobacillus CGMCC No.9663 to patulin scavenging action in the aqueous solution
1st, the preparation of patulin standard items mother liquor
With embodiment 1.
2nd, the preparation of the solution example of the patulin containing various concentrations
As needed, the patulin standard items mother liquor of certain volume is accurately measured, pH 4.0 is dissolved in after being dried up with nitrogen
In water, and it is settled to 100mL.As compound concentration be 1000 μ g/L patulin nutrient solution when, accurately measuring concentration first is
100mg/L patulin standard items 1mL, is dissolved in the water of pH 4.0 after being dried up with nitrogen, is settled to 100mL, that is, obtains concentration
For 1000 μ g/L patulin solution example；Compound concentration is that 500 μ g/L, 1500 μ g/L and 2000 μ g/L patulin are molten
During liquid sample, it is respectively necessary for taking 0.5mL, 1.5mL and 2mL patulin standard mother liquor.
3rd, analyses of the lactobacillus CGMCC No.9663 to patulin Scavenging activity is digested under different time
Collect 1 × 1010CFU thalline and 1mL concentration are that 1000 μ g/L patulins solution examples in temperature are 25 DEG C, are turned
Speed is co-culturing taking-up in 0.5 hour, 4 hours, 8 hours, 12 hours and 24 hours respectively to be co-cultured in 150r/min shaking table
Reacted sample, supernatant is taken in 4 DEG C, 12000rpm rotating speed centrifugation 10min, with 0.22 μm of membrane filtration, using efficient
The residual condition of patulin in liquid chromatographic detection sample.3 Duplicate Samples are done in the processing of each time.It is meanwhile each different
The patulin treatment group of time is respectively provided with a control group for being not added with digesting lactobacillus CGMCC No.9663.
Testing conditions：Chromatographic column Unitary C18,150mm × 4.6mm × 5 μm；Mobile phase is methanol：Water=10:90,
Flow velocity 1ml/min, detector are UV-detector, Detection wavelength 276nm, and applied sample amount is 20 μ L, the appearance time of patulin
For 8.1min-10.1min.
Experimental result：Digest scavenging action versus time curves of the lactobacillus CGMCC No.9663 to patulin
Such as Fig. 2, digested at 4 hours lactobacillus treatment group to the clearance rate of patulin be 88.93%, 8 hours after it is reachable
Digest lactobacillus CGMCC No.9663 after 94.29%, 12 hours not change significantly the elimination effect of patulin, 24
Clearance rate during hour is 98.89%.
Embodiment 5, which digests lactobacillus CGMCC No.9663, reduces the content of patulin in cider
It is 1000 μ g/mL that patulin is added by solvent of commercially available cider and adjusts its concentration, adds 1010CFU/mL digests
Lactobacillus CGMCC No.9663 biological agents, mix and taken out after 24h is incubated in 25 DEG C, the shaking table that rotating speed is 150r/min.
The patulin extracted after taking-up in cider determines its content using high performance liquid chromatography.Set simultaneously and do not add digestion lactobacillus
The blank group of CGMCC No.9663 biological agents.Specific extracting method is as follows：
Ciders of the 5-10mL containing patulin is taken, adds 20mL ethyl acetate, 2min is shaken, is collected after static layering
Upper organic phase, repeat above step twice, merge 3 organic phases, add 4mL 1.5% sodium carbonate liquor shaking 1min
(this step will fast, prevent that penicillium expansum element is unstable in the basic conditions), after stratification, sodium carbonate layer uses 10mL acetic acid again
Ethyl ester extracts once.Scavenging solution after extraction is poured into 100mL pear shape bottle, in rotary evaporation in 40 DEG C of water-baths to 1~
2mL, concentrate is moved in centrifuge tube, dried up in 40 DEG C of nitrogen, with 1.0mL pH 4.0 acetic acid aqueous solution dissolution residual substance,
After 0.45 μm of membrane filtration, for liquid chromatogram measuring.
Experimental result such as Fig. 3, digestion lactobacillus CGMCC No.9663 can significantly reduce containing for patulin in cider
Amount, the content of patulin in apple can be dropped to 54.41 μ g/L from 966.95 μ g/L, clearance rate is up to 94.37%.
Embodiment 6, which digests lactobacillus CGMCC No.9663, reduces the content of patulin in mouldy pomace
A certain amount of pomace is weighed, is put into plate, is wrapped with brown paper, high pressure steam sterilization；Pomace sterilizing is cold
But after, the penicillium expansum element spore suspension of patulin can be produced by inoculum concentration 2-5% accesses, is uniformly mixed；By nothing
Bacterium water presses material-water ratio 1:0.5~1 adds in pomace, is averagely dispensed into sterilizing plates after the mixing that stirs, wherein three
1-2% digestion lactobacillus CGMCC No.9663 are inoculated with individual plate respectively, digestion lactobacillus is added without in blank plate, will connect
Kind digests the plate of lactobacillus and does not access the blank plate for digesting lactobacillus heat-preservation fermentation 3-5 days at a temperature of 25-35 DEG C,
After having fermented, the patulin in pomace, extracting method such as embodiment 5 are extracted.With high performance liquid chromatography detection exhibition after extraction
The content of penicillin.
Experimental result such as Fig. 4, it can be seen that adding digestion lactobacillus CGMCC in mouldy pomace
No.9663 can effectively reduce the content of patulin, and the amount of patulin in pomace is dropped to from 652.72 μ g/kg
105.09μg/kg。
Embodiment 7 prepares the lactobacillus CGMCC No.9663 cornstalk silages containing digestion
Corn straw feedstuff raw material is rubbed and is switched to stand-by after 1-4cm, then weighs 50kg corn straw feedstuff raw materials,
0.6g digestion lactobacillus CGMCC No.9663 working stock cultures are well mixed with 220mL sterilized waters, are then charged into ensilage bucket
In be pressed and sealed, after then being stored 10 months under normal temperature condition i.e. obtain containing digestion lactobacillus CCFM 636 maize straw raise
Material, compared with being not added with digesting the corn straw feedstuff of lactobacillus, the content of patulin significantly reduces.
Embodiment 8 digests lactobacillus CGMCC No.9663 acquisition
Take 1mL to pat the homogenate of uniform fermentation vegetables and be added to enrichment culture in 30mL MRS culture mediums, 37 DEG C of culture 20h.
Gradient dilution is coated on the MRS flat boards of 0.02% bromocresol purple of addition.Picking becomes the obvious single bacterium colony of chromosphere, rules repeatedly
To the single bacterium colony of purifying, choosing colony diameter is between 1~3mm, rounded protuberance, and surface is smooth or slightly coarse, it is milky white, greyish white or
Dark yellow etc. meets the bacterial strain of lactic acid bacteria colony characteristicses, carries out Gram's staining, selects Gram-positive, without gemma bacterial strain.
With 2% inoculum concentration by strain passage twice after, centrifugation 10min (5000g ×, 4 DEG C) collects thalline, then uses phosphate
Buffer solution (pH 7.2) washing centrifugation 2 times.The thalline of acquisition is resuspended in the physiology salt that 1mL concentration containing PAT is 1000 μ g L-1
In water (pH 4.0), adjustment bacteria concentration is placed in 37 DEG C of trainings in the shaking table that rotating speed is 150r min-1 in 1010CFU mL-1 or so
Sampled after supporting 24h, centrifugation 10min (12000g ×, 4 DEG C), take supernatant to use height after being filtered using 0.22 μm of miillpore filter
Effect liquid phase chromatogram instrument (HPLC) detects PAT remaining concentration.The same concentration PAT solution for not adding thalline is set simultaneously as negative right
According to.So as to filter out the stronger patulin bacterial strain CCFM 636 of patulin Scavenging activity, identified preservation, digestion breast is obtained
Bacillus CGMCC No.9663.
Although the present invention is disclosed as above with preferred embodiment, it is not limited to the present invention, any to be familiar with this skill
The people of art, without departing from the spirit and scope of the present invention, it can all do various change and modification, therefore the protection model of the present invention
Enclose being defined of being defined by claims.
Claims (10)
1. one kind digestion lactobacillus (Lactobacillus alimentarius), the bacterial strain is in preservation on the 15th in 09 month in 2014
In China Committee for Culture Collection of Microorganisms's common micro-organisms center, preservation address is BeiChen West Road, Chaoyang District, BeiJing City 1
Number No. 3 Institute of Microorganism, Academia Sinica of institute, deposit number is CGMCC No.9663.
A kind of 2. biology prepared using digestion lactobacillus CGMCC No.9663 described in claim 1 for removing patulin
The method of preparation, it is characterised in that be that centrifugation after the digestion lactobacillus activation culture is obtained into digestion lactobacillus thalline, washing
Thalline is collected afterwards to obtain digesting lactobacillus biological agent.
3. according to the method for claim 2, it is characterised in that the thalline protective agent for further obtaining collection, which is resuspended, to be reached
To concentration 1010CFU/mL, suspension culture is freeze-dried afterwards for a period of time to obtain digestion lactobacillus bacterium powder biological agent.
4. according to the method for claim 3, it is characterised in that described protective agent is skimmed milk power, trehalose or sucrose.
5. a kind of contain the biological agent that lactobacillus CGMCC No.9663 are digested described in claim 1.
6. answering in terms of patulin of the biological agent described in claim 5 in the removing aqueous solution, fruit juice and food and feed
With.
7. lactobacillus CGMCC No.9663 answering in the patulin in removing food or feed is digested described in claim 1
Use method.
8. application processes of the lactobacillus CGMCC No.9663 in animal feed is produced is digested described in claim 1.
9. according to the method for claim 8, it is characterised in that the animal feed is that fermentation of apple pulp feed or ensiling are raised
Material.
10. applications of the lactobacillus CGMCC No.9663 in patulin antidote is prepared is digested described in claim 1.
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| CN106148210B (en) * | 2015-03-23 | 2019-12-13 | 中国海洋大学 | Korotka ohmer yeast for degrading patulin and application thereof |
| CN109456917B (en) * | 2018-11-21 | 2020-09-18 | 中国科学院植物研究所 | TT-09 bacteria for degrading patulin and application thereof |
| CN109527339A (en) * | 2018-11-30 | 2019-03-29 | 东北农业大学 | A method of utilizing patulin in Lactobacillus rhamnosus removal fluid sample |
| CN112391297B (en) * | 2019-08-13 | 2021-12-31 | 中国农业大学 | Candida utilis for degrading patulin, biological preparation and application thereof |
| CN111909878A (en) * | 2020-08-22 | 2020-11-10 | 扬州大学 | Lactobacillus casei with function of removing patulin |
| CN111909884B (en) * | 2020-09-22 | 2022-05-17 | 内蒙古农业大学 | Lactobacillus plantarum and application thereof |
| CN113558159A (en) * | 2021-07-09 | 2021-10-29 | 西北农林科技大学 | Preparation process of fermented apple juice |
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|---|---|---|---|---|
| EP0973416B1 (en) * | 1997-04-03 | 2004-09-22 | Bucher-Alimentech Ltd. | Process for reducing the patulin concentration in fruit juices |
| CN102578676A (en) * | 2012-02-24 | 2012-07-18 | 中国科学院植物研究所 | Application of Candida guilliermondii strain to elimination of patulin |
| CN102578675A (en) * | 2012-02-24 | 2012-07-18 | 中国科学院植物研究所 | Application of Pichia membranaefaciens strain to elimination of patulin |
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2014
- 2014-12-05 CN CN201410734478.7A patent/CN104403973B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0973416B1 (en) * | 1997-04-03 | 2004-09-22 | Bucher-Alimentech Ltd. | Process for reducing the patulin concentration in fruit juices |
| CN102578676A (en) * | 2012-02-24 | 2012-07-18 | 中国科学院植物研究所 | Application of Candida guilliermondii strain to elimination of patulin |
| CN102578675A (en) * | 2012-02-24 | 2012-07-18 | 中国科学院植物研究所 | Application of Pichia membranaefaciens strain to elimination of patulin |
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