CN106561977A - Composite type synbiotic microecological preparation for cattle and preparation method of composite type synbiotic microecological preparation - Google Patents
Composite type synbiotic microecological preparation for cattle and preparation method of composite type synbiotic microecological preparation Download PDFInfo
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- CN106561977A CN106561977A CN201610995509.3A CN201610995509A CN106561977A CN 106561977 A CN106561977 A CN 106561977A CN 201610995509 A CN201610995509 A CN 201610995509A CN 106561977 A CN106561977 A CN 106561977A
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- 235000019722 synbiotics Nutrition 0.000 title abstract 8
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- 239000006041 probiotic Substances 0.000 claims abstract description 86
- 235000018291 probiotics Nutrition 0.000 claims abstract description 86
- 229920001542 oligosaccharide Polymers 0.000 claims abstract description 29
- 150000002482 oligosaccharides Chemical class 0.000 claims abstract description 28
- 235000013406 prebiotics Nutrition 0.000 claims abstract description 19
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims abstract description 18
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- WQZGKKKJIJFFOK-QTVWNMPRSA-N D-mannopyranose Chemical compound OC[C@H]1OC(O)[C@@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-QTVWNMPRSA-N 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/175—Rhamnosus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/51—Bifidobacterium
Abstract
The invention discloses a composite type synbiotic microecological preparation for cattle and a preparation method of the composite type synbiotic microecological preparation. The composite type synbiotic microecological preparation consists of probiotics and prebiotics, wherein the probiotics and the prebiotics are mixed in the mass ratio of the probiotics to the prebiotics being 1 to (1-5); the probiotics consist of bacterium lacticum, bifidobacteria, bacillus subtilis and lactobacillus rhamnosus; and the prebiotics consist of oligosaccharides and an acidulant, wherein the oligosaccharides are manoligosaccharides, fructo-oligosaccharides, isomaltooligosacharides and xylo-oligosaccharides, and the acidulant is a composite acidulant consisting of lactic acid, citric acid and fumaric acid. According to the composite type synbiotic microecological preparation and the preparation method thereof disclosed by the invention, the disadvantage of singly using probiotics products when commodity beef cattle, cows and the like are raised is overcome, the composite type synbiotic microecological preparation is suitable for all kinds of commodity cattle, and the colonization of exogenous probiotics in intestinal tracts of animals is promoted; and besides, the rapid growth and propagation of various probiotics in cattle bodies are selectively stimulated, so that the purpose of promoting the health of the cattle is achieved, the production properties of the cattle are also greatly improved, the influence of conventional animal edible additives on the quality of beef and the quality of milk is reduced, and the composite type synbiotic microecological preparation is economic and practical.
Description
Technical field
The invention belongs to field of biological, more particularly to a kind of cattle compound symphysis unit microbial ecological agent and its preparation
Method.
Background technology
The appearance of microbial ecological agent is that, due to the improvement of people's living standards, people gradually recognize that antibiotic adds for a long time
The health of the mankind can be worked the mischief by food chain in feedstuff.At present, life of China's symphysis unit microbial ecological agent in animal
Applied research in product is also less, and in farm animals edible additive, the service condition of microbial ecological agent is also less, people couple
Microbial ecological agent is suitable for addition and relatively obscures, that is, obscure, cause the effect of growth of animal often low
In Expected Results.The research application of microbial ecological agent overcomes a variety of drawbacks that life-time service antibiotic is caused to aquaculture, right
Development green feed additive has broad application prospects, and oneself is classified as 21st century emphasis promotional technique item by the Ministry of Agriculture
Mesh.This means that the research to microbial ecological agent, popularization meet the general requirement of China's sustainable development.In document " Kang Feng, Lu
Win bright, Zhang Haifeng, An Ligang, Hu Jianwu. functional oligosaccharide and its mechanism of action. Laboratory Animal Science .2011 (28). " in, refer to
The functional oligosaccharide gone out in microbial ecological agent is a kind of safe, new green environment protection additive for having no toxic side effect, Neng Gougai
Microecological environment in kind animal intestinal, promotes profitable strain growth, suppresses the breeding of pathogen, improve animal immunizing power, from
And promote animal health growth.In document model on one's own account. application of the functional oligose in husbandry sector. grain and feedstuff work
Industry, 2000 (11):In 32-33. ", author points out oligosaccharide as the excellent succedaneum of antibiotic, for the production of cattle,
Improve its premunition, reduce feedstuff-meat ratio, raising and give milk in terms of rate and weightening etc. that there is preferably effect.
The defect of prior art is that the stability of bacterial strain in microbial ecological agent is not ensured well, is thrown necessary to bacterium
Gut metabolism is reached by stomach in the form of viable bacteria, and colonization is trained in intestinal and grown, this antibacterial to general spore form comes
Say and be not easily achieved.Furthermore microbial ecological agent does not targetedly add object, and the domestic application to microbial ecological agent is also not
Enough abundant, not enough, the not a height of solution abuse of antibiotics of the degree that received by raiser causes increasing pathogenic popularizations degree
Bacterium develops immunity to drugs, drug residue, reduces beef, dairy product quality and as food chain is accumulated to human body health care belt
That what is come has a strong impact on, and produces the microbial ecological agent of substitute antibiotics.
The content of the invention
It is an object of the invention to provide a kind of cattle compound symphysis unit microbial ecological agent and preparation method thereof, it is intended to solve
Certainly animal is low with symphysis unit microbial ecological agent absorption rate at present, the problem of application effect difference.
The present invention is achieved in that a kind of compound symphysis unit microbial ecological agent of cattle, the compound symphysis unit of the cattle
Microbial ecological agent component by weight is cattle gastrointestinal tract probiotic bacteria, oligosaccharide；Cattle gastrointestinal tract probiotic bacteria is by weight component by Fructus rhamni (Rhamnus davurica Pall.)
(reference culture is purchased from Beijing North and receives to create and joins biological skill sugared lactobacilluss, bacillus subtilises+lactobacilluss and bacillus bifiduss composition
Art academy)；
Cattle gastrointestinal tract probiotic bacteria is 0.2-1.5g/kg, and probiotic bacteria nutrient substance is 0.5-8g/kg, and oligosaccharide is 0.1-9g/
kg。
Another object of the present invention is to a kind of preparation method of cattle with compound symphysis unit microbial ecological agent is provided,
The cattle preparation method of compound symphysis unit microbial ecological agent, specifically includes：
Step one, the soil from the plant of cattle, feed for nursing groove, gastrointestinal region separate rhamnose breast bar
Isolated probiotic bacteria is carried out biological characteristicses, function and peace by bacterium, bacillus subtilises, lactobacilluss and bacillus bifiduss
The identification of full property, and carry out external probiotic effects evaluation, preliminary screening probiotic bacteria；
Step 2, using cattle enteric microorganism and carbohydrate metabolism, the relation of vitamin, filter out with obtaining for energy can be improved
Take and store, improve the high-quality probiotic bacteria that cattle obtains the ability of energy from daily ration, using raising of the probiotic bacteria in Intestinum Bovis seu Bubali road
The acquisition and storage of energy, raising cattle obtain the ability of energy from daily ration, according to microbial technique index screening high-quality cattle stomach
Beneficial bacteria of intestinal tract；
Step 3, the probiotic bacteria in the cattle gastrointestinal fluid of manual simulation after culture preliminary screening, determine all kinds of probiotic bacterias
Viable count, reproductive order of generation, it is double that selection obtains 1 plant of lactobacillus rhamnosus, 1 bacillus subtilis, 3 plants of lactobacilluss and 1 plant
Discrimination bacillus；
Step 4, the species according to probiotic bacteria, the functional characteristic of all kinds of prebioticses and the utilization journey with corresponding probiotic bacteria
Degree selects the prebioticses being made up of oligosaccharide, draws the optimum addition of all kinds of oligosaccharide；
Step 5, to add oligosaccharide after probiotic bacteria according to mass ratio 0.1%-0.5% add by 3 parts of weight breast
Acid, the citric acid of 1 part of weight, the compound acidulant of 1 part of Fumaric acid composition；
Step 6, the prebioticses of 6 probiotics and mass ratio 5% after addition compound acidulant are combined, preparation
Go out cattle with compound symphysis unit microbial ecological agent.
Further, in the optimal bacterial strain of step 3 screening preferably：1 plant of lactobacillus rhamnosus, 1 bacillus subtilis, 3 plants
Lactobacilluss and 1 plant of bacillus bifidus.
Further, in step 4, all kinds of oligosaccharide are：Xylooligosaccharide, MOS, fruit oligose, oligomeric isomaltose；
The preferred xylooligosaccharide 0.05-5g/kg of all kinds of oligosaccharide optimum additions, MOS 0.25-2g/kg, fruit oligose
0.02-2g/kg, oligomeric isomaltose 0.5-3g/kg；
Nutrient substance：Each accounting example is the Vc of 0.001%-2%, VE, zinc, iodine, chromium, NaHCO3、KHCO3, Ramulus Uncariae Cum Uncis, chlorination
Sodium, bile saltss, Fructus Crataegi and Fructus Hordei Germinatus.
The cattle of the present invention is avoided that the weakness that probiotic products are used alone, phase with compound symphysis unit microbial ecological agent
It is auxiliary to coordinate, maximize favourable factors and minimize unfavourable ones, promote field planting of the exogenous probiotic bacteria in Intestinum Bovis seu Bubali road, and the life of the various probiotic bacterias of selective stimulating
Long and breeding, so as to reach the purpose for promoting animal health, reduces impact of the animal edible antibiotic to beef matter, is more good for
Kang Anquan, it is nuisanceless, growth of animal not only can be promoted, moreover it is possible to improve the conversion ratio of feedstuff, increase measurement techniques for quality detection of meat and improve food
The safe mass of product, selects suitable prebioticses according to all kinds of probiotic bacterias, solves the medicine brought by traditional antibiotic
Thing is remained, is developed immunity to drugs, animal intestinal micro-ecology balance is destroyed and reduces the food safetys such as meat productss quality and quality
Problem.
Description of the drawings
Fig. 1 is preparation method flow chart of the cattle provided in an embodiment of the present invention with compound symphysis unit microbial ecological agent.
Specific embodiment
For the content of the invention, feature and effect of the present invention can be further appreciated that, following examples are hereby enumerated, and coordinates accompanying drawing
Describe in detail as follows：
Preparation method of the cattle provided in an embodiment of the present invention with compound symphysis unit microbial ecological agent, first respectively in difference
In the environment of filter out cattle gastrointestinal tract probiotic bacteria, by the function and biological characteristicses ratio of the cattle gastrointestinal tract probiotic bacteria to filtering out
Compared with, and simulation intestinal environment under compare probiotic bacteria viable count change, filter out optimal bacterial strain, all kinds of oligosaccharide contained
Amount is measured, and selects optimum addition, and cattle gastrointestinal tract probiotic bacteria and oligosaccharide are mixed the micro- life of compound symphysis unit
State preparation.
As shown in figure 1, cattle provided in an embodiment of the present invention is concrete with the preparation method of compound symphysis unit microbial ecological agent
Including：
S101, the soil from the plant of cattle, feed for nursing groove, gastrointestinal region separate rhamnose breast bar
Isolated probiotic bacteria is carried out biological characteristicses, function and peace by bacterium, bacillus subtilises, lactobacilluss and bacillus bifiduss
The identification of full property, and carry out external probiotic effects evaluation, preliminary screening probiotic bacteria；
S102, using cattle enteric microorganism and carbohydrate metabolism, the relation of vitamin, filter out with the acquisition that can improve energy
The high-quality probiotic bacteria of the ability of energy, the raising energy using probiotic bacteria in Intestinum Bovis seu Bubali road are obtained with storage, raising cattle from daily ration
The acquisition and storage of amount, raising cattle obtain the ability of energy from daily ration, according to microbial technique index screening high-quality cattle gastrointestinal
Road probiotic bacteria；
S103, the probiotic bacteria in the cattle gastrointestinal fluid of manual simulation after culture preliminary screening, determine all kinds of probiotic bacterias
Viable count, reproductive order of generation are further screened, and final selection obtains 1 plant of lactobacillus rhamnosus, 1 plant of bacillus subtilis
Bacterium, 3 plants of lactobacilluss and 1 plant of bacillus bifidus；
S104, the species according to probiotic bacteria, the functional characteristic of all kinds of prebioticses and the producing level with corresponding probiotic bacteria
The prebioticses that selection is made up of oligosaccharide, show that the optimum addition of all kinds of oligosaccharide is xylooligosaccharide 0.05-5g/kg, few manna
Sugared 0.25-2g/kg, fruit oligose 0.02-2g/kg, oligomeric isomaltose 0.5-3g/kg；
S105, to add oligosaccharide after probiotic bacteria according to mass ratio 0.1%-0.5% add by 3 parts of weight breast
Acid, the citric acid of 1 part of weight, the compound acidulant of 1 part of Fumaric acid composition；
S106,6 probiotics and the prebioticses of mass ratio 5% after addition compound acidulant are combined, are made
Cattle is with compound symphysis unit microbial ecological agent.
Using cattle gastrointestinol microorganism and carbohydrate metabolism, the relation of vitamin in this embodiment, raising cattle is filtered out
The high-quality probiotic bacteria of energy can be obtained from daily ration, the acquisition and storage of the raising energy using high-quality probiotic bacteria in cattle gastrointestinal tract
The ability deposited, further according to microbial technique index selection-breeding probiotic bacteria；1. probiotic bacteria is separated on selective medium, then to institute
Every plant of antibacterial of choosing carries out the physiological property detection of acidproof, bile tolerance, digestion resistant enzyme etc. one by one, and other are special finally to carry out probiotic bacteria
Property experiment.2. a small amount of content is taken in animal alimentary canal first, and be diluted, under anaerobic state, entered in MRS culture medium
Row enrichment culture, then obtain can gradually increase the acidity of culture medium by gradient culture again as the antibacterial of prebiotic strain
(pH is 3.0 or so) and bile salt concentration, select the bacterial strain with acidproof and resistance to bile characteristic, wanting further according to probiotic properties
Ask, add other inhibitor or pathogen in the medium, filter out and grow fine and the bacterial strain for killing pathogen can be pressed down, finally
Carry out the forms such as Gram’s staining, catalase and biochemical identification again to the bacterial strain, and determine its kind.Screening high-quality cattle gastrointestinal tract benefit
Raw bacterium；3. the identification that isolated probiotic bacteria is carried out biological characteristicses, the comparison of function and safety is screened and determines,
And carry out external probiotic effects evaluation, preliminary screening high-quality probiotic bacteria；1) lactobacilluss (as a example by screening from animal gastrointestinal tract)：Point
Bis- Zhi Chang ﹑ Fei Chang ﹑ Kong Chang ﹑ cecal contents of other Qu Wei ﹑ Shi are a certain amount of serial in 10 times of 50mL anaerobism diluent Zhong ﹑ Jiao Ban ﹑ works
Dilution, takes each 0.1mL of 3 dilution factors respectively and is coated with LBS flat boards, 37-38 DEG C, anaerobic jar culture.Chosen from flat board with inoculating loop
Taking all kinds bacterium colony carries out gram stain microscopy, and the bacterium colony with typical lactobacilluss morphological characteristic is screened.In LBS
, in 0.5mm-2mm, smooth Shi Run ﹑ edges Zheng Qi ﹑ are raised on Bai Se ﹑ Yuan Xing ﹑ surfaces for the diameter that culture 48-72h is observed on flat board
Bacterium colony be lactobacilluss.2) bacillus bifiduss (as a example by screening from animal gastrointestinal tract)：Qu Wei ﹑ bis- Zhi Chang ﹑ Fei Chang ﹑ of Shi are empty respectively
Chang ﹑ cecal contents are a certain amount of to be dissolved in 100mL modified MRS culture mediums, and enrichment culture 24h takes 1mL gradient dilutions, coats flat
On plate, 48h being cultivated under anaerobic, being purified 3 times with inoculating loop picking blue colonies, obtain bacillus bifiduss, picking is strictly detested
The bacterial strain of oxygen carries out whether Gram’s staining, microscopy observation thalline Yan Se ﹑ great little ﹑ shapes etc. meet the substantially special of Bifidobacterium
Levy.3) bacillus subtilises (as a example by screening from animal gastrointestinal tract) take the finger intestinal ﹑ fertilizer intestinal ﹑ sky intestinal ﹑ caecal contents of stomach ﹑ 12 respectively
Thing is a certain amount of to be made 10 times in 50mL anaerobism diluent Zhong ﹑ Jiao Ban ﹑ and is serially diluted, and takes each 0.1mL of 3 dilution factors respectively and is coated with LBS
Flat board, 37 DEG C of culture 48h-72h, is suspected to be bacterium colony with inoculating loop picking, first carries out azaleine and simply dye observation, then carry out gram
Dyeing and spore staining, picking bacillus subtilises bacterium colony carry out pure culture, and preserve in inclined-plane, then carry out catalase survey
It is fixed, will each separation strains be inoculated in LB culture medium slants, 37 DEG C of cultures
18h-24h, takes a clean slide, in the H for dripping 10% above2O2, the cultured lawn of one ring of picking,
H2O2In smear, if there is bubble, for the positive, otherwise for feminine gender.4) lactobacillus rhamnosuss (Lactobacillus rhamnosus
GG) LGG be Grain-positive anaerobe, plasmid-free；Lactose can not be utilized, but can metabolism monosaccharide；Can give birth to very well under anaerobic conditions
It is long, there is CO2In the presence of can also grow.It is special that LGG grows presentation in LBS tomato juice agars and MRS agar culture mediums
Fixed morphological feature, this makes it easier to identification, in both culture medium LGG in big, creamy-white, opaque colony,
And distribute creamy taste.Its concrete isolation identification step participates in 1) lactobacilluss (by taking the soil near plant as an example)：
(1) acquisition process of soil
Sample in soil using five point sampling near plant.As the microorganism in soil is typically in 5-25cm
Soil layer in, so first rooting out the epidermis of soil with sterilizing, then take soil 200-300g, as in aseptic plastic bag,
And indicate and take place, date.Preserve as in refrigerator after being transported back laboratory, it is standby.
(2) in soil strain separation
The sample adopted back is obtained into sample 20-30g with quartering, grinding is uniform in being placed on mortar.1g soil is weighed,
It is placed in fluid medium sterilizing Sucus Vitis viniferae, 28 DEG C of Jing, 12h, 180rpm shaking table enrichment culture.1mL enrichment culture liquid is taken, is entered respectively
Five gradient dilutions (1 of row:1000、1:5000、1:10000、1:50000、1:100000), then with liquid-transfering gun respectively from each dilute
Degree of releasing respectively takes 0.1mL diluents, and on injection flat board, sample is uniformly coated in media surface using spreading rod, and (attention can not
Rupture media surface), culture dish is covered, then culture dish is inverted, in preference temperature (generally 25 DEG C or 28 DEG C)
Cultivate in calorstat, each gradient does three repetitions.Observation culture medium, till strain forms the bacterium colony clearly easily debated.For
The interference of antibacterial is excluded in screening process, in the medium plus 30ug/mL streptomycin or 100mg/L chloromycetin.
Colony colour and form are observed and record, and then cell size, form are recorded in microexamination, are analyzed and return with preliminary
Class.
The application principle of the present invention is further described with reference to specific embodiment.
Embodiment 1：Soil preliminary screening bacillus category probiotic bacteria near plant.
The first step：Collection containing bacillus cereuss pedotheque.The soil near large-scale plant that raises is selected to be collection destination,
3-5 cell of random selection, each cell take 20-50g initial soils, load in sterilized valve bag, take back laboratory and put
Save backup in 4 DEG C of refrigerators；
Second step：The separation of bacillus cereuss.Aseptically, enrichment culture liquid is added in pedotheque, form bacterium
Fall, then strain is inoculated into into middle addition physiological saline solution and make bacteria suspension, then be diluted to 10 sequence diminishing methods different dense
Degree gradient, different solubility gradient samples is carried out into plate count, each dilution factor do 3 it is parallel, thus made by put down
Plate cultivates 24-72h in being put into couveuse；
3rd step：Bacillus cereuss are isolated and purified.Aseptically, the probiotic bacteria after discriminating is connect with inoculating loop
Plant in physiological saline solution, make bacteria suspension, be inoculated on flat board, observe after culture, circulate operation, until obtaining pure bacterium
Fall, inclined plane inoculating is saved backup；
4th step：The discriminating of bacillus cereuss.Bacillus cereuss after isolating and purifying are carried out into morphologic observation, physio-biochemical characteristics
Determine and 16S rDNA sequencings etc. method, respectively different bacterium colonies is identified, the mesh for knowing that to which class distinguishes name is reached
's.
Embodiment 2：Preliminary screening lactobacilluss, bacillus bifiduss probiotic bacteria from animal intestinal.
The first step：Collection containing probiotic bacteria sample.Select each large-scale slaughterhouse to be sample collecting destination, cut at random
Different health now kill the intestinal tissues of animal, load in sterilized valve bag, and quickly take back laboratory and are placed in 4 DEG C of refrigerators
Save backup；
Second step：The enrichment of probiotic bacteria, separation.Aseptically, intestinal tissue sample inner surface material is added to
Cultivate in enrichment culture liquid, form bacterium colony, then strain is inoculated into into addition physiological saline solution and make bacteria suspension, then use 10 sequences
Diminishing method is diluted to different Concentraton gradient, and different solubility gradient samples is carried out plate count, and each dilution factor does 3
It is individual parallel, thus made by flat board be put into couveuse in cultivate 24-72h；
3rd step：Probiotic bacteria is isolated and purified.Aseptically, the probiotic bacteria after discriminating is inoculated with inoculating loop
In physiological saline solution, bacteria suspension is made, be inoculated on flat board, observed after culture, circulate operation, until obtaining pure bacterium
Fall, inclined plane inoculating is saved backup；
4th step：The discriminating of probiotic bacteria.Lactobacilluss after isolating and purifying are carried out into morphologic observation, physio-biochemical characteristics are determined
With 16S rDNA sequencings etc. method, respectively different bacterium colonies is identified, the purpose for knowing that to which class distinguishes name is reached.
Embodiment 3：Lactobacillus rhamnosuss are separated from the Pickles of natural fermentation.
The first step：The selection of the Pickles of natural fermentation.The Pickles that farmers' voluntarily rises are selected for collection destination, selection use
Make Pickles sour water as sample 30-50mL, load in sterilized valve bag, take back laboratory and be placed in 4 DEG C of refrigerators and preserve standby
With；
Second step：The enrichment of lactobacillus rhamnosuss, initial gross separation.Aseptically, sour water sample is added to into enrichment
In culture fluid, bacterium colony is formed, then strain is inoculated into into physiological saline solution and make bacteria suspension, then be diluted to 10 sequence diminishing methods
Different solubility gradient samples is carried out plate count by different Concentraton gradient, each dilution factor do 3 it is parallel, thus
Made by flat board be put into couveuse in cultivate 24-72h；
3rd step：Lactobacillus rhamnosuss are isolated and purified.Aseptically, by the lactobacillus rhamnosuss after discriminating
It is inoculated in physiological saline solution with inoculating loop, makes bacteria suspension, be inoculated on flat board, is observed after culture, circulate operation, directly
To the bacterium colony that purebred lactobacillus rhamnosuss are formed is obtained, inclined plane inoculating is saved backup；
4th step：The discriminating of lactobacillus rhamnosuss.Lactobacillus rhamnosuss after isolating and purifying are carried out into morphologic observation, physiology
Biochemical characteristic determine and 16S rDNA sequencings etc. method, respectively different bacterium colonies is identified, is reached and class is known to which
Distinguish the purpose of name.
Embodiment 4：The further screening of probiotics strain.
The first step：The probiotic bacteria of preliminary screening is carried out into function test measure respectively, for example, the probiotic bacteria of new culture is connect
Plant in the bile salt culture-medium of variable concentrations, determine Bile salt resistance；Probiotic bacteria is cultivated 3-5 days in the environment of low pH, then
Judge the acidproof degree of probiotic bacteria；
Second step：The probiotic bacteria after preliminary screening is cultivated in manual simulation gastrointestinal microecological environment, to all kinds of benefits
The survival rate of raw bacterium, reproductive order of generation are measured, and filter out the preferable probiotic bacteria of upgrowth situation；
3rd step：The above is every to determine the probiotic bacteria that qualified probiotic bacteria is final screening, finally gives 1 plant of rhamnose breast
Bacillus, 3 plants of lactobacilluss, 1 plant of bacillus bifidus and 1 bacillus subtilis；
Embodiment 5：The preparation of compound symphysis unit microbial ecological agent.
The first step：Species, the functional characteristic of all kinds of prebioticses according to probiotic bacteria and the utilization journey with corresponding probiotic bacteria
Degree selects the prebioticses that are made up of oligosaccharide and acidulant, and selectable oligosaccharide is xylooligosaccharide, MOS, and fruit oligose is low
IMO etc., acidulant is using the compound acidulant by material compositions such as lactic acid, citric acid, Fumaric acids
Second step：The addition of all kinds of oligosaccharide is tested, the addition of all kinds of oligosaccharide is xylooligosaccharide 0.05-5g/kg, few
Mannose 0.5-2g/kg, fruit oligose 0.01-3g/kg, oligomeric isomaltose 0.01-2g/kg.Add by lactic acid, citric acid, prolong
The compound acidulant 0.1%-0.5% of the material compositions such as fumarate；
3rd step：Five kinds of probiotic bacterias and the prebioticses selected are combined, adding is beneficial to be conducive to benefit
The nutrient substance of raw bacteria growing, makes compound symphysis unit microbial ecological agent；
4th step：The product being eventually fabricated carries out zoopery in being added to animal edible material, according to the increasing of laboratory animal
Weight, feed consumption and feedstuff-meat ratio etc. carry out the production performance situation of judgment experiment animal.
The compound symphysis unit microbial ecological agent of the cattle of the present invention is avoided that the weakness that probiotic products are used alone, mutually auxiliary
Coordinate, maximize favourable factors and minimize unfavourable ones, promote field planting of the exogenous probiotic bacteria in Intestinum Bovis seu Bubali road, and the growth of the various probiotic bacterias of selective stimulating
And breeding, so as to reach the purpose for promoting animal health, impact of the animal edible antibiotic to beef matter is reduced, it is more healthy
Safety, it is nuisanceless, growth of animal not only can be promoted, moreover it is possible to improve the conversion ratio of feedstuff, increase measurement techniques for quality detection of meat and improve food
Safe mass, select suitable prebioticses according to all kinds of probiotic bacterias, solve the medicine brought by traditional antibiotic
Remain, develop immunity to drugs, animal intestinal micro-ecology balance is destroyed and reduces the food safetys such as meat productss quality and quality is asked
Topic.
The above is only, to presently preferred embodiments of the present invention, not to make any pro forma restriction to the present invention,
Every technical spirit according to the present invention is belonged to any simple modification made for any of the above embodiments, equivalent variations and modification
In the range of technical solution of the present invention.
Claims (4)
1. a kind of cattle is with compound symphysis unit microbial ecological agent, it is characterised in that the cattle is with compound symphysis unit microbial ecological agent
Component is cattle gastrointestinal tract probiotic bacteria, oligosaccharide by weight；Cattle gastrointestinal tract probiotic bacteria is by weight component by lactobacillus rhamnosuss, withered
Careless bacillus cereuss+lactobacilluss and bacillus bifiduss composition；
Cattle gastrointestinal tract probiotic bacteria is 0.2-1.5g/kg, and probiotic bacteria nutrient substance is 0.5-8g/kg, and oligosaccharide is 0.1-9g/kg.
2. preparation method of a kind of cattle as claimed in claim 1 with compound symphysis unit microbial ecological agent, it is characterised in that described
Cattle is specifically included with the preparation method of compound symphysis unit microbial ecological agent：
Step one, the soil from the plant of cattle, feed for nursing groove, gastrointestinal region separate lactobacillus rhamnosuss,
Isolated probiotic bacteria is carried out biological characteristicses, function and safety by bacillus subtilises, lactobacilluss and bacillus bifiduss
Identification, and carry out external probiotic effects evaluation, preliminary screening probiotic bacteria；
Step 2, using cattle enteric microorganism and carbohydrate metabolism, the relation of vitamin, filter out with the acquisition that can improve energy and
Storage, raising cattle obtain the high-quality probiotic bacteria of the ability of energy, the raising energy using probiotic bacteria in Intestinum Bovis seu Bubali road from daily ration
Acquisition and storage, improve cattle the ability of energy obtained from daily ration, according to microbial technique index screening high-quality cattle gastrointestinal tract
Probiotic bacteria；
Step 3, the probiotic bacteria in the cattle gastrointestinal fluid of manual simulation after culture preliminary screening, determine the work of all kinds of probiotic bacterias
Bacterium number, reproductive order of generation, selection obtain 1 plant of lactobacillus rhamnosus, 1 bacillus subtilis, 3 plants of lactobacilluss and 1 plant of bifid bar
Bacterium；
Step 4, the species according to probiotic bacteria, the functional characteristic of all kinds of prebioticses and the producing level choosing with corresponding probiotic bacteria
The prebioticses being made up of oligosaccharide are selected, the optimum addition of all kinds of oligosaccharide is drawn；
Step 5, to add oligosaccharide after probiotic bacteria according to mass ratio 0.1%-0.5% add by 3 parts of weight lactic acid, 1
The compound acidulant of the citric acid of part weight, 1 part of Fumaric acid composition；
Step 6,6 probiotics and the prebioticses of mass ratio 5% after addition compound acidulant are combined, make cattle
With compound symphysis unit microbial ecological agent.
3. preparation method of the cattle as claimed in claim 2 with compound symphysis unit microbial ecological agent, it is characterised in that step 3
In the optimal bacterial strain of screening preferably：1 plant of lactobacillus rhamnosus, 1 bacillus subtilis, 3 plants of lactobacilluss and 1 plant of bifid bar
Bacterium.
4. preparation method of the cattle as claimed in claim 2 with compound symphysis unit microbial ecological agent, it is characterised in that step 4
In all kinds of oligosaccharide be：Xylooligosaccharide, MOS, fruit oligose, oligomeric isomaltose；
The preferred xylooligosaccharide 0.05-5g/kg of all kinds of oligosaccharide optimum additions, MOS 0.25-2g/kg, fruit oligose 0.02-
2g/kg, oligomeric isomaltose 0.5-3g/kg；
Nutrient substance：Each accounting example is the Vc of 0.001%-2%, VE, zinc, iodine, chromium, NaHCO3、KHCO3, Ramulus Uncariae Cum Uncis, Sodium Chloride, gallbladder
Juice salt, Fructus Crataegi and Fructus Hordei Germinatus.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109287874A (en) * | 2018-11-29 | 2019-02-01 | 佛山市南海东方澳龙制药有限公司 | For promoting the additive and its preparation method and application of ruminant cub growth and development |
| CN111733107A (en) * | 2020-07-07 | 2020-10-02 | 安徽农业大学 | Bovine-derived composite micro-ecological preparation and application thereof |
| CN116473193A (en) * | 2023-04-27 | 2023-07-25 | 安徽省豆谷生物工程有限公司 | Bean yogurt and preparation method thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101406489A (en) * | 2008-11-28 | 2009-04-15 | 黑龙江省科学院微生物研究所 | Micro-ecological complex bacterial agent and method of preparing micro-ecological complex bacterial agent |
| CN102488114A (en) * | 2011-12-15 | 2012-06-13 | 天津工业大学 | Microbial feed additive production method |
| CN102726606A (en) * | 2012-03-20 | 2012-10-17 | 重庆市畜牧科学院 | Micro-ecological synbiotic feed addictive, preparation method and application thereof |
| CN103262954A (en) * | 2013-04-19 | 2013-08-28 | 安徽强农牧业有限公司 | Fermented dairy feed and preparation method thereof |
| CN103315139A (en) * | 2013-07-01 | 2013-09-25 | 乌鲁木齐思科康达科技有限责任公司 | Compound microbial ecological agent for monogastric animals and preparation method thereof |
-
2016
- 2016-11-10 CN CN201610995509.3A patent/CN106561977A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101406489A (en) * | 2008-11-28 | 2009-04-15 | 黑龙江省科学院微生物研究所 | Micro-ecological complex bacterial agent and method of preparing micro-ecological complex bacterial agent |
| CN102488114A (en) * | 2011-12-15 | 2012-06-13 | 天津工业大学 | Microbial feed additive production method |
| CN102726606A (en) * | 2012-03-20 | 2012-10-17 | 重庆市畜牧科学院 | Micro-ecological synbiotic feed addictive, preparation method and application thereof |
| CN103262954A (en) * | 2013-04-19 | 2013-08-28 | 安徽强农牧业有限公司 | Fermented dairy feed and preparation method thereof |
| CN103315139A (en) * | 2013-07-01 | 2013-09-25 | 乌鲁木齐思科康达科技有限责任公司 | Compound microbial ecological agent for monogastric animals and preparation method thereof |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109287874A (en) * | 2018-11-29 | 2019-02-01 | 佛山市南海东方澳龙制药有限公司 | For promoting the additive and its preparation method and application of ruminant cub growth and development |
| CN111733107A (en) * | 2020-07-07 | 2020-10-02 | 安徽农业大学 | Bovine-derived composite micro-ecological preparation and application thereof |
| CN116473193A (en) * | 2023-04-27 | 2023-07-25 | 安徽省豆谷生物工程有限公司 | Bean yogurt and preparation method thereof |
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