CN105193905A - Traditional Chinese medicine processing technology of ganoderma-astragalus membranaceus bidirectional fermentation - Google Patents
Traditional Chinese medicine processing technology of ganoderma-astragalus membranaceus bidirectional fermentation Download PDFInfo
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- CN105193905A CN105193905A CN201410296409.2A CN201410296409A CN105193905A CN 105193905 A CN105193905 A CN 105193905A CN 201410296409 A CN201410296409 A CN 201410296409A CN 105193905 A CN105193905 A CN 105193905A
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- astragalus membranaceus
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Abstract
The invention discloses a traditional Chinese medicine processing technology of ganoderma-astragalus membranaceus bidirectional fermentation. The traditional Chinese medicine processing technology comprises the procedures of astragalus membranaceus pre-processing, ganoderma strain activation, bidirectional fermentation culture medium preparation, bidirectional fermentation, medicine property change and fermentation mycoplasm processing. The traditional Chinese medicine processing technology has the advantages that the biological activities and medicine effects of both ganoderma and astragalus membranaceus are enhanced according to a bidirectional fermentation technology, the antioxidant activity of astragalus membranaceus is improved by 20% or above after fermentation, and astragalus membranaceus is easy to absorb. Therefore, the processed traditional Chinese medicine can serve as a functional food or medicine for enhancing resistance and prolonging the lifetime, and has a good application prospect.
Description
Technical field
The invention belongs to technical field of bioengineering, relating to a kind of take Chinese medicine as the two-way fermentation technique of microorganism of raw material.
Background technology
Gansu has abundant medicine resource, but is generally still at present based on crude drug and elementary processed goods supply market, and active constituent content is low, and value-rising is few, also cannot ensure that market is to high-end and multifarious demand; The task of top priority is the tackling of key scientific and technical problems carrying out genuine medicinal materials, improves the value of Chinese crude drug; The Radix Angelicae Sinensis in Gansu, Radix Codonopsis, Huang (red) stilbene, Radix Et Rhizoma Rhei, Radix Glycyrrhizae output account for 95%, 60%, 50%, 60%, 25% of the whole nation respectively, and the export volume of above 5 kinds of medical materials accounts for more than 80% of the whole nation; The Radix Astragali
radixAstragalibeing the common medicine of tcm clinical practice, is the dry root of leguminous plant Radix Astagali or Radix Astragali; The Radix Astragali has effect of diuresis detoxification, evacuation of pus, expelling pus and promoting granulation.Main chemical compositions has flavonoid, saponins and polysaccharide, in addition also containing compositions such as monosaccharide, aminoacid, protein, folic acid, vitamin D, linoleic acid, Palmic acid, cetyl alcohol;
Ganoderma
ganodermaLucidumbe the famous and precious fungus of China's tradition, be the valuable ingredient of Chinese medicine with strengthening by means of tonics, strengthening the body resistance, clothes have effect of life lengthening for a long time; Scientific research confirm Ganoderma have improve immunity, antitumor, anticancer, ease pain, protect the liver, hypermnesis, antibacterial, antioxidation, antiinflammatory, the pharmacologically active such as antimutagenic; The auxiliary treatment etc. of Ganoderma to chronic bronchitis, hat sexually transmitted disease (STD), hepatitis, liver cirrhosis, arteriosclerosis, hyperlipidemia, hypertension, neurasthenia, oncosis has curative effect in various degree clinically; The various active material such as polysaccharide, triterpenes, furans, alkaloids, aminoacid protein class, oils, inorganic ions, sterols contained in these pharmacologically actives and Ganoderma is closely related; The demand of Ganoderma constantly increases in recent years, and wild Ganoderma is very rare, can not meet the needs of people far away, and Ganoderma Lucidum growth cycle is long; Utilize fermentation engineering can make Ganoderma raising with short production cycle, active; Namely two-way fermentation is seeded in certain substrate with certain medicinal strain, under certain environment condition, through certain hour fermentation, controls to be issued to fermentation termination and produce mycoplasma in specific quality index; Chinese medicine can be utilized as the ingredient of culture medium, build medicinal fungal substance, for break-through point is found in the combination of Chinese medicine and medicinal fungi, and exploitation has the medicine of good effects.
Summary of the invention
Chinese medicine two-way fermentation technique system adopts different Chinese crude drugs to be that substrate forms different medicinal mycoplasma, or in nutrient matrix, add the Chinese crude drug composition wholeness matrix with active component, or become multiformity (compound recipe) substrate with two or more Chinese crude drug through combined crosswise, by inoculating different strains as medicinal fungi, make the effect of fermentation from originally unidirectional only by C, the nutritional labelings such as N source, the enzyme developing into application strain makes tissue and the ingredient breakdown of medicinal mycoplasma, change, produce new composition, Fermentation is made to develop into amphicheirality, thus form new nature and flavor, the medicinal fungal substance that function is different or full property mycoplasma, therefore there is amphicheirality or multiformity, the Radix Astragali of this problem using main product in Minxian County, Gansu, as dominant fermentation substrate, with Ganoderma as strain, adopts the two-way fermentation technique of fungus to ferment, is intended to the bioactive ingredients of both increases and strengthens drug effect, especially strengthening its anti-oxidation efficacy, utilize Ganderma lucidum can multiple exoenzyme such as eccrine fiber element enzyme, peroxidase, superoxide dismutase, esterase etc. in fermentation, make it act on the Radix Astragali to improve its medicine eduction rate, increase the change of secondary metabolite, the object of the invention is to realize by following technical scheme, it is characterized in that it comprises the following steps:
(1) Radix Astragali pretreatment: after Radix Astragali clean dry, section, grinds into 4-40 order size powder;
(2) Ganderma lucidum strain activation: Linzhi is activated by PDA slant medium, 28 DEG C of constant temperature culture 7 days; Semen Maydis powder fluid medium amplification culture 4 days, treats that mycelia covers with inclined-plane for subsequent use;
(3) two-way fermentation medium preparation: astragalus membranaceus powder is 5-15%, and Semen Maydis and corn cob compound are 83%-93%(corn cob: Semen Maydis powder=1:3), MgSO
4(0.5%), (NH
4)
2sO
4(0.5%), KH
2pO
4(1%), mass/mass concentration is more than; By above-mentioned substance mix after, add its gross weight 65% water stir after sterilizing for subsequent use;
(4) two-way fermentation: the Ganoderma after activation is accessed above-mentioned culture medium, with solid-state or liquid form fermentation, optimum temperature 28 degree, Ganoderma inoculum concentration 12%(12mL/100g), pH=5.5-6.5, ventilation dark culturing 7-13d;
(5) property of medicine change: when Ganoderma covers with whole fermentation tank, and its mycelium pellet is spherical in shape, when outward appearance is dense, pure white, namely fermentation comes to an end; Before and after Radix Astragali by Solid Fermentation, DPPH free radical scavenging activity is respectively 61.21%, 75.41%, improves 23.20% before comparatively fermenting after fermentation; Hydroxyl radical free radical clearance rate is respectively 59.24%, 71.08%, improves 19.99% before comparatively fermenting after fermentation; Ganoderma Biomass (g)=10.58/100ml, mycelium pellet diameter (mm)=4.9; Mycelia outward appearance is dense, pure white;
(6) process of Zymocyte: to the centrifugal 15min of fermentation liquid under 4000r/min condition, getting supernatant is final products; The homogenizing process of solid fermentation thing: under 45-65 DEG C of condition, tunning (comprising mixture and the mycoplasma of mycelia and culture matrix composition) is dried to constant weight, cross 40 mesh sieves after pulverizing, permeate is final products;
beneficial effect of the present invention
(1) technique is simple, and equipment requirements is not high, is suitable for industrialization promotion;
(2) utilize the property of medicine and functional component specific to the Radix Astragali and Ganoderma, interacted by the profound level of the two medical health care functions such as lifting its antioxidation, free radical resisting, enhancing immunity;
(3) the above packing of product is become glossy ganoderma fermentation dry powder and oral liquid can as building up resistance, the merit medicine of slow down aging, life lengthening and functional food, application prospect is good.
Detailed description of the invention
The following examples can further illustrate the present invention, but do not limit the present invention in any way
embodiment 1
Solid Double is to fermentation---and take the drying Radix Astragali some, grind to form 40 order size powder by pulverizer; Take Ganoderma as fermentation medicinal fungi seed, PDA slant medium activates, and adopts Semen Maydis powder fluid medium amplification culture; By adding the Radix Astragali powder of 15% (accounting for the full weight amount of culture medium) concentration in the basal medium of setting, make full property culture medium (corn cob: Semen Maydis powder=1:3, MgSO
4(0.5%), (NH
4)
2sO
4(0.5%), KH
2pO
4(1%), mass concentration is).Access Ganoderma seed liquor, is placed at 28 DEG C, controls oxygen-supply quantity by force ventilation, under natural Ph condition, cultivates 13d; When Ganoderma covers with whole fermentation tank, and its mycelium pellet is spherical in shape, and when outward appearance is dense, pure white, namely fermentation comes to an end;
embodiment 2
Liquid two-way fermentation---take the drying Radix Astragali some, be cut into the square size bar of 0.5cm*2cm by microtome, then add 9 times of mass aqueous solution, stir and soak 3 hours; Then the centrifugal 15min of 1000r-3000r/min, gets supernatant; Take Ganoderma as fermentation medicinal fungi seed, give birth to Ganderma lucidum strain two pieces with aseptic inoculation, be inoculated in slant medium (PDA culture medium) middle part, be placed in 28 DEG C of constant incubators and cultivate 7 days; Adopt Semen Maydis powder fluid medium amplification culture; By adding the Radix Astragali aqueous extract of 30% concentration in the basal medium of setting, make full property culture medium (corn cob: Semen Maydis powder=1:3, MgSO
4(0.5%), (NH
4)
2sO
4(0.5%), KH
2pO
4(1%), mass concentration is); Access Ganoderma seed liquor, is placed at 28 DEG C, shaking speed 150-180r/min, under natural Ph condition, cultivates 7d; When Ganoderma covers with whole fermentation tank, and its mycelium pellet is spherical in shape, and when outward appearance is dense, pure white, namely fermentation comes to an end;
embodiment 3
Liquid two-way fermentation---take the drying Radix Astragali some, grind to form 40 order size powder by pulverizer; Take Ganoderma as fermentation medicinal fungi seed, give birth to Ganderma lucidum strain two pieces with aseptic inoculation, be inoculated in slant medium (PDA culture medium) middle part, be placed in 28 DEG C of constant incubators and cultivate 7 days; Adopt Semen Maydis powder fluid medium amplification culture; By adding the Radix Astragali powder of 5% (accounting for the full weight amount of culture medium) concentration in the basal medium of setting, make full property culture medium (corn cob: Semen Maydis powder=1:3, MgSO
4(0.5%), (NH
4)
2sO
4(0.5%), KH
2pO
4(1%), mass concentration is); Access Ganoderma seed liquor, is placed at 28 DEG C, shaking speed 150r/min, under natural Ph condition, cultivates 7d; When Ganoderma covers with whole fermentation tank, and its mycelium pellet is spherical in shape, and when outward appearance is dense, pure white, namely fermentation comes to an end.
Claims (1)
1. a processing technique of Chinese herbs for the two-way fermentation of Ganoderma-Radix Astragali, is characterized in that step is as follows:
(1) Radix Astragali pretreatment: after Radix Astragali clean dry, section, grinds into 4-40 order size powder;
(2) Ganderma lucidum strain activation: Linzhi is activated by PDA slant medium, 28 DEG C of constant temperature culture 7 days; Semen Maydis powder fluid medium amplification culture 4 days, treats that mycelia covers with inclined-plane for subsequent use;
(3) two-way fermentation medium preparation: astragalus membranaceus powder is 5-15%, and Semen Maydis and corn cob compound are 83%-93%(corn cob: Semen Maydis powder=1:3), MgSO
4(0.5%), (NH
4)
2sO
4(0.5%), KH
2pO
4(1%), mass/mass concentration is more than; By above-mentioned substance mix after, add its gross weight 65% water stir after sterilizing for subsequent use
(4) two-way fermentation: the Linzhi after activation is accessed above-mentioned culture medium, with solid-state or liquid form fermentation, optimum temperature 28 degree, Ganoderma inoculum concentration 12%(12mL/100g), pH=5.5-6.5,65 DEG C of ventilation dark culturing 7-13d;
(5) property of medicine change: when Ganoderma covers with whole fermentation tank, and its mycelium pellet is spherical in shape, when outward appearance is dense, pure white, namely fermentation comes to an end; Before and after Radix Astragali by Solid Fermentation, DPPH free radical scavenging activity is respectively 61.21%, 75.41%, improves 23.20% before comparatively fermenting after fermentation; Hydroxyl radical free radical clearance rate is respectively 59.24%, 71.08%, improves 19.99% before comparatively fermenting after fermentation; Ganoderma Biomass (g)=10.58/100ml, mycelium pellet diameter (mm)=4.9; Mycelia outward appearance is dense, pure white;
(6) process of Zymocyte: to the centrifugal 15min of fermentation liquid under 4000r/min condition, getting supernatant is final products; The homogenizing process of solid fermentation thing: dried to constant weight by tunning (comprising mixture and the mycoplasma of mycelia and culture matrix composition) under 45-65 DEG C of condition, cross 40 mesh sieves after pulverizing, permeate is final products.
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106820134A (en) * | 2017-02-21 | 2017-06-13 | 南京晓庄学院 | A kind of preparation method and purposes of the immunopotentiator based on sesame stilbene mycoplasma |
| CN106852954A (en) * | 2017-02-19 | 2017-06-16 | 哈尔滨伟平科技开发有限公司 | A kind of preparation method of ganoderma lucidum and radix astragali oral liquid |
| CN107549449A (en) * | 2017-10-20 | 2018-01-09 | 遵义医学院 | A kind of astragalus root dregs biological feed additive and preparation method and application |
| CN109439719A (en) * | 2018-11-27 | 2019-03-08 | 广东中测食品化妆品安全评价中心有限公司 | A kind of fungi two-way solid-fermented technique and its application based on Cortex Eucommiae |
| CN110218656A (en) * | 2019-05-31 | 2019-09-10 | 南京工业大学 | The two-way solid fermentation method of ganoderma lucidum-dregs of a decoction and application using air pressure pulsation |
| CN110302129A (en) * | 2019-08-21 | 2019-10-08 | 云南澳玛生物技术有限公司 | One kind educating hair anticreep scalp nursing solution |
| CN110755344A (en) * | 2019-10-25 | 2020-02-07 | 北京京瑜科技有限公司 | Ganoderma lucidum-rhizoma polygonati bidirectional fermentation process and composition |
| CN112586604A (en) * | 2020-08-12 | 2021-04-02 | 淮阴师范学院 | Method and device for preparing feed additive through ganoderma-astragalus bidirectional fermentation based on pressure pulsation technology |
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| CN103316058A (en) * | 2013-05-22 | 2013-09-25 | 甘肃农业大学 | Technology for improving drug effects of Mythic Fungus and Chinese angelica through utilizing Mythic Fungus-Chinese angelica bidirectional fermentation |
| CN103565944A (en) * | 2013-11-20 | 2014-02-12 | 青岛农业大学 | Bi-directional fermentation technology for traditional Chinese medicine |
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2014
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Patent Citations (2)
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| CN103316058A (en) * | 2013-05-22 | 2013-09-25 | 甘肃农业大学 | Technology for improving drug effects of Mythic Fungus and Chinese angelica through utilizing Mythic Fungus-Chinese angelica bidirectional fermentation |
| CN103565944A (en) * | 2013-11-20 | 2014-02-12 | 青岛农业大学 | Bi-directional fermentation technology for traditional Chinese medicine |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106852954A (en) * | 2017-02-19 | 2017-06-16 | 哈尔滨伟平科技开发有限公司 | A kind of preparation method of ganoderma lucidum and radix astragali oral liquid |
| CN106820134A (en) * | 2017-02-21 | 2017-06-13 | 南京晓庄学院 | A kind of preparation method and purposes of the immunopotentiator based on sesame stilbene mycoplasma |
| CN107549449A (en) * | 2017-10-20 | 2018-01-09 | 遵义医学院 | A kind of astragalus root dregs biological feed additive and preparation method and application |
| CN109439719A (en) * | 2018-11-27 | 2019-03-08 | 广东中测食品化妆品安全评价中心有限公司 | A kind of fungi two-way solid-fermented technique and its application based on Cortex Eucommiae |
| CN110218656A (en) * | 2019-05-31 | 2019-09-10 | 南京工业大学 | The two-way solid fermentation method of ganoderma lucidum-dregs of a decoction and application using air pressure pulsation |
| CN110218656B (en) * | 2019-05-31 | 2022-05-17 | 南京工业大学 | Ganoderma lucidum-herb residue bidirectional solid fermentation method utilizing air pressure pulsation and application |
| CN110302129A (en) * | 2019-08-21 | 2019-10-08 | 云南澳玛生物技术有限公司 | One kind educating hair anticreep scalp nursing solution |
| CN110755344A (en) * | 2019-10-25 | 2020-02-07 | 北京京瑜科技有限公司 | Ganoderma lucidum-rhizoma polygonati bidirectional fermentation process and composition |
| CN112586604A (en) * | 2020-08-12 | 2021-04-02 | 淮阴师范学院 | Method and device for preparing feed additive through ganoderma-astragalus bidirectional fermentation based on pressure pulsation technology |
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