CN105543105A - Fungus strain capable of promoting salidroside accumulation of rhodiola crenulata and application of fungus strain - Google Patents

Fungus strain capable of promoting salidroside accumulation of rhodiola crenulata and application of fungus strain Download PDF

Info

Publication number
CN105543105A
CN105543105A CN201610011872.7A CN201610011872A CN105543105A CN 105543105 A CN105543105 A CN 105543105A CN 201610011872 A CN201610011872 A CN 201610011872A CN 105543105 A CN105543105 A CN 105543105A
Authority
CN
China
Prior art keywords
substratum
radix rhodiolae
mycelia
seedling
accumulation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CN201610011872.7A
Other languages
Chinese (zh)
Other versions
CN105543105B (en
Inventor
崔晋龙
焦晋
王梦亮
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Shanxi University
Original Assignee
Shanxi University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Shanxi University filed Critical Shanxi University
Priority to CN201610011872.7A priority Critical patent/CN105543105B/en
Publication of CN105543105A publication Critical patent/CN105543105A/en
Application granted granted Critical
Publication of CN105543105B publication Critical patent/CN105543105B/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12RINDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00Microorganisms ; Processes using microorganisms
    • C12R2001/645Fungi ; Processes using fungi
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14Fungi; Culture media therefor
    • C12N1/145Fungal isolates
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00Preparation of compounds containing saccharide radicals
    • C12P19/44Preparation of O-glycosides, e.g. glucosides

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Botany (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Biomedical Technology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The invention discloses a trimmatostroma betulinum fungus strain, and the preservation number is CGMCC No.11417. By co-culturing the fungus strain and a rhodiola crenulata tissue culture seedling or adding a mycelium or a fermentation liquor extract generated through liquid fermentation into a culture medium of the rhodiola crenulata tissue culture seedling, salidroside accumulation of the rhodiola crenulata tissue culture seedling can be promoted. The fungus strain has the important application prospect on rhodiola crenulata resource introduction, promotion of salidroside accumulation of the rhodiola crenulata and the like.

Description

Promote fungal bacterial strain and the application thereof of Radix Rhodiolae accumulation rhodioside
Technical field
The present invention relates to functionally active microorganism, be specifically related to birch powder seat spore fungal bacterial strain, and this fungal bacterial strain and tunning thereof are promoting the application in Radix Rhodiolae tissue cultured seedling accumulation rhodioside.
Background technology
Radix Rhodiolae [Rhodiolacrenulata (Hook.f.etThoms.) S.H.Fu] is the per nnial herb of Rosales Crassulaceae rhodiola.Mainly being distributed in Himalaya area, being grown on height above sea level more than high and cold, anoxic, the intense light irradiation of 3000m, windy mountain area, is the national medicinal plants using more than one thousand years such as local Tibetan.Along with going deep into of research, Radix Rhodiolae medicinal material is widely used in the fields such as medicine, healthcare products, makeup, is the unique Original plant going through the Root of Kirilow Rhodiola medicinal material that version " Chinese Pharmacopoeia " is included.
The chemical composition of Radix Rhodiolae comprises benzene alkylglycosides as styroyl glycoside, tyrosol, rhodioside; Phenylpropanoid Glycosides glycoside (network plug dimension); Phenolic glycoside (kaempferol 7-O--alpha-L-rhamnoside); Flavonoid (glycosides compound of flavonol and formation thereof, as herbaceous stem element 7-O-alpha-L-rhamnoside); Organic acid (as gallic acid), polysaccharide, amino acid, terpenes, alcohols and the inorganic elements such as fats and selenium.Traditional drugs with in, Radix Rhodiolae is mainly used in the treatment of the aspects such as anti-hypoxia, anti-oxidant, antifatigue.Pharmacology activity research shows, rhodioside is one of main active ingredient of Radix Rhodiolae, and the height of its content is as evaluating, and Root of Kirilow Rhodiola kind is good and bad, the important indicator of the Root of Kirilow Rhodiola medicinal material true and false.
At present, the supply of Radix Rhodiolae medicinal material is mainly derived from wild resource, but only has the Determination of Salidroside of perennial rhodiola plant just can reach medicinal standard; Another source be alpine region introduce a fine variety plantation product, even but the Determination of Salidroside accumulation medicinal material of too slow more than 5 years, also lower than wild samples contg; Along with the development of tissue culture technique, people attempt in the wild condition of low altitude area condition Imitating, carry out the extensive tissue culture of Root of Kirilow Rhodiola, but the subject matter faced remain the subject matters such as Determination of Salidroside is on the low side, and accumulating rate is too slow.Therefore, the supply of Root of Kirilow Rhodiola medicinal material is seriously hindered.
The present invention (namely relies at breakthrough conventional art and changes temperature, pH value, illumination, CO 2the condition such as concentration, humidity), utilize fungi and plant symbiosis, the strain that screening obtains is under low altitude area condition, the activity fungal of Root of Kirilow Rhodiola accumulation rhodioside is promoted in the shorter time, this technology has the features such as green is controlled, with low cost, method is novel, and this fungal strain is a kind of biotic induce factor of effective promotion rhodioside accumulation.
Summary of the invention
The invention reside in the biological activity fungi providing a strain can promote Radix Rhodiolae tissue cultured seedling accumulation main active ingredient rhodioside within a short period of time.
A fungal strain ZPRS-R-11 provided by the invention, through being accredited as birch powder seat spore (Trimmatostromabetulinum) bacterium, this fungal strain is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center on November 6th, 2015, deposit number is CGMCCNo.11417, depositary institution address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica.
The cultural characters of this fungal strain is: cultivate on PDA substratum, and bacterium colony is grey, and mycelia is velveteen shape, central uplift, and mycelia is flourishing, in hair shape edge, without visible secretory product; Bacterium colony back side chocolate, central authorities are dark, and edge is shallow; This bacteria growing speed is partially slow, and the colony diameter growing two weeks is 5cm; Under microscopy conditions, mycelia is in dark-coloured and light color two kinds, and light color mycelia is very thin, and dark-coloured mycelia is sturdy, and diameter, between 1-2 μm, often exists rhizomorph, and mycelia content is high-visible, mycelia without every, tool branch, does not produce spore.
A kind of method promoting the rhodioside Rapid Accumulation organizing training Radix Rhodiolae seedling provided by the invention, it is the bacterial strain activation of CGMCCNo.11417 by deposit number, be inoculated in PDA solid plate substratum, when growth to about plate diameter 2/3 time, with punch tool, bacterium cake is broken into the bacterium cake that diameter is 5 ~ 15mm, get 3 ~ 5 pieces, be evenly inverted on the substratum be put in around Root of Kirilow Rhodiola tissue cultured seedling stem, this substratum is containing 0.3 ~ 1.0mg/LTDZ; 0.3 ~ 1.0mg/LNAA, carries out Dual culture, 15 ~ 25 days, and compared to contrast, the Determination of Salidroside of symbiosis seedling improves greatly.
A kind of method promoting the rhodioside Rapid Accumulation organizing training Radix Rhodiolae seedling provided by the invention, it is the bacterial strain activation of CGMCCNo.11417 by deposit number, be inoculated in PDA solid plate substratum, when growth to about plate diameter 2/3 time, with punch tool, bacterium cake is broken into the bacterium cake that diameter is 5 ~ 15mm, 5 ~ 6 ferfas cakes are inoculated in the triangular flask that the sterilized PDA liquid nutrient medium of 200mL is housed, 25 DEG C ~ 30 DEG C, cultivate 7 ~ 10 days under 150-200rpm condition, by filtered through gauze, obtain mycelia and culture fermentation broth; Mycelia is made mycelia powder under 45 DEG C of conditions, culture fermentation broth is made paste under 45 DEG C of conditions; According to the ratio that weight and volume are 1 ~ 10 ︰ 1000 (g ︰ mL), mycelia and fermented liquid are joined MS substratum respectively (containing 0.3-1.0mg/LTDZ; 0.3-1.0mg/LNAA), inoculation Radix Rhodiolae tissue cultured seedling, cultivate 15 ~ 25 days, compared to contrast, Determination of Salidroside improves greatly.
Compared with prior art, advantage of the present invention and effect:
The present invention adopts deposit number to be birch powder seat spore (Trimmatostromabetulinum) fungi of CGMCCNo.11417 and culture thereof and Root of Kirilow Rhodiola seedling Dual culture, can make Determination of Salidroside Rapid Accumulation.The method breaks through conventional means, adopts microorganism and plant Coculture techniques, and novel, convenient, controlled, development potentiality greatly, is solution Determination of Salidroside good method on the low side.
Embodiment
In embodiment, fungi ZPRS-R-11 is birch powder seat spore (Trimmatostromabetulinum) fungi that deposit number is CGMCCNo.11417.
Embodiment 1
Bacterial strain is activated, be inoculated in PDA solid plate substratum, when growth to about plate diameter 2/3 time, with punch tool, bacterium cake is broken into the bacterium cake that diameter is 5mm, get 3 pieces, be evenly inverted the substratum that is put in around Root of Kirilow Rhodiola tissue cultured seedling stem (containing 0.3mg/LTDZ; 0.3mg/LNAA), carry out Dual culture, after 15 days, the HPLC method according to " Chinese Pharmacopoeia " P144 page record in 2010 measures Determination of Salidroside.Concrete outcome is in table 1.
Embodiment 2
Bacterial strain is activated, be inoculated in PDA solid plate substratum, when growth to about plate diameter 2/3 time, with punch tool, bacterium cake is broken into the bacterium cake that diameter is 15mm, get 5 pieces, be evenly inverted the substratum that is put in around Root of Kirilow Rhodiola tissue cultured seedling stem (containing 1.0mg/LTDZ; 1.0mg/LNAA), carry out Dual culture, after 25 days, the HPLC method according to " Chinese Pharmacopoeia " P144 page record in 2010 measures Determination of Salidroside.Concrete outcome is in table 1.
Embodiment 3
Be that the pure culture biscuits involvng inoculation of 5mm is in the triangular flask that the sterilized PDA liquid nutrient medium of 200mL is housed by 5 pieces of diameters, 25 DEG C, cultivate 7 days under 150rpm condition, by 4 layers of filtered through gauze, obtain mycelium culture thing, dry under 45 DEG C of conditions, grind into powder, is the ratio of 1 ︰ 1000 (g ︰ mL) according to weight and volume, mycelia powder is joined MS substratum (containing 1.0mg/LTDZ; 0.3mg/LNAA), inoculation Radix Rhodiolae tissue cultured seedling, cultivates 15 days, and the HPLC method according to " Chinese Pharmacopoeia " P144 page record in 2010 measures Determination of Salidroside.Concrete outcome is in table 1.
Embodiment 4
Be that the pure culture biscuits involvng inoculation of 15mm is in the triangular flask that the sterilized PDA liquid nutrient medium of 200mL is housed by 6 pieces of diameters, 30 DEG C, cultivate 10 days under 200rpm condition, by 4 layers of filtered through gauze, obtain mycelium culture thing, dry under 45 DEG C of conditions, grind into powder, is the ratio of 10 ︰ 1000 (g ︰ mL) according to weight and volume, mycelia powder is joined MS substratum (containing 3.0mg/LTDZ; 1.0mg/LNAA), inoculation Radix Rhodiolae tissue cultured seedling, cultivates 25 days, and the HPLC method according to " Chinese Pharmacopoeia " P144 page record in 2010 measures Determination of Salidroside.Concrete outcome is in table 1.
Embodiment 5
Be that the pure culture biscuits involvng inoculation of 5mm is in the triangular flask that the sterilized PDA liquid nutrient medium of 200mL is housed by 5 pieces of diameters, 25 DEG C, cultivate 7 days under 150rpm condition, by 4 layers of filtered through gauze, obtain fermented liquid, 45 DEG C of condition rotary evaporations become paste, according to the ratio that weight and volume are 1 ︰ 1000 (g ︰ mL), mycelia powder is joined MS substratum (containing 1.0mg/LTDZ; 0.3mg/LNAA), inoculation Radix Rhodiolae tissue cultured seedling, cultivates 15 days, and the HPLC method according to " Chinese Pharmacopoeia " P144 page record in 2010 measures Determination of Salidroside.Concrete outcome is in table 1.
Embodiment 6
Be that the pure culture biscuits involvng inoculation of 5mm is in the triangular flask that the sterilized PDA liquid nutrient medium of 200mL is housed by 6 pieces of diameters, 30 DEG C, cultivate 10 days under 200rpm condition, by 4 layers of filtered through gauze, obtain fermented liquid, 45 DEG C of condition rotary evaporations become paste, according to the ratio that weight and volume are 10 ︰ 1000 (g ︰ mL), mycelia powder is joined MS substratum (containing 0.3mg/LTDZ; 1.0mg/LNAA), inoculation Radix Rhodiolae tissue cultured seedling, cultivates 25 days, and the HPLC method according to " Chinese Pharmacopoeia " P144 page record in 2010 measures Determination of Salidroside.Concrete outcome is in table 1.
Comparative example 1
Blank: adopt MS substratum (containing 0.5mg/LTDZ; 0.5mg/LNAA), inoculation Radix Rhodiolae tissue cultured seedling, cultivates 20 days, and the HPLC method according to " Chinese Pharmacopoeia " P144 page record in 2010 measures Determination of Salidroside.Concrete outcome is in table 1.
Comparative example 2
PDA liquid nutrient medium 45 DEG C of condition rotary evaporations are become paste by negative control: when not connecing fungi, be the ratio of 5 ︰ 1000 (g ︰ mL), PDA paste joined MS substratum (containing 0.5mg/LTDZ according to weight and volume; 0.5mg/LNAA), inoculation Radix Rhodiolae tissue cultured seedling, cultivates 20 days, and the HPLC method according to " Chinese Pharmacopoeia " P144 page record in 2010 measures Determination of Salidroside.Concrete outcome is in table 1.
Table 1. fungi promotes that Radix Rhodiolae improves rhodioside and tyrosol content
Rhodioside (mg/g)
Embodiment 1 2.216
Embodiment 1 2.309
Embodiment 1 2.782
Embodiment 1 2.752
Embodiment 1 1.963
Embodiment 6 2.351
Comparative example 1 1.450
Comparative example 2 1.487

Claims (5)

1. birch powder seat spore (Trimmatostromabetulinum) fungal bacterial strain, its deposit number is CGMCCNo.11417.
2. the application of fungal bacterial strain in the accumulation promoting Determination of Salidroside in Radix Rhodiolae tissue cultured seedling as claimed in claim 1.
3. one kind promotes the method for the rhodioside Rapid Accumulation organizing training Radix Rhodiolae seedling, step comprises: by fungal bacterial strain activation described in claim 1, be inoculated in PDA solid plate substratum, when growth to about plate diameter 2/3 time, with punch tool, bacterium cake is broken into the bacterium cake that diameter is 5 ~ 15mm, get 3 ~ 5 pieces, be evenly inverted on the substratum be put in around Root of Kirilow Rhodiola tissue cultured seedling stem, this substratum is containing 0.3 ~ 1.0mg/LTDZ; 0.3 ~ 1.0mg/LNAA, carries out Dual culture, 15 ~ 25 days.
4. one kind promotes the method for the rhodioside Rapid Accumulation organizing training Radix Rhodiolae seedling, step comprises: by fungal bacterial strain activation described in claim 1, be inoculated in PDA solid plate substratum, when growth to about plate diameter 2/3 time, with punch tool, bacterium cake is broken into the bacterium cake that diameter is 5 ~ 15mm, 5 ~ 6 ferfas cakes are inoculated in the triangular flask that the sterilized PDA liquid nutrient medium of 200mL is housed, 25 DEG C ~ 30 DEG C, cultivate 7 ~ 10 days under 150-200rpm condition, by filtered through gauze, obtain mycelia and culture fermentation broth; Mycelia is made mycelia powder under 45 DEG C of conditions, is the ratio of 1 ~ 10 ︰ 1000 (g ︰ mL), is joined by mycelia powder in MS substratum according to weight and volume, this substratum is containing 0.3-1.0mg/LTDZ; 0.3-1.0mg/LNAA, inoculation Radix Rhodiolae tissue cultured seedling, cultivates 15 ~ 25 days.
5. one kind promotes the method for the rhodioside Rapid Accumulation organizing training Radix Rhodiolae seedling, step comprises: by fungal bacterial strain activation described in claim 1, be inoculated in PDA solid plate substratum, when growth to about plate diameter 2/3 time, with punch tool, bacterium cake is broken into the bacterium cake that diameter is 5 ~ 15mm, 5 ~ 6 ferfas cakes are inoculated in the triangular flask that the sterilized PDA liquid nutrient medium of 200mL is housed, 25 DEG C ~ 30 DEG C, cultivate 7 ~ 10 days under 150-200rpm condition, by filtered through gauze, obtain mycelia and culture fermentation broth; Culture fermentation broth is made paste under 45 DEG C of conditions; According to the ratio that weight and volume are 1 ~ 10 ︰ 1000 (g ︰ mL), joined respectively by paste in MS substratum, this substratum is containing 0.3-1.0mg/LTDZ, 0.3-1.0mg/LNAA, and inoculation Radix Rhodiolae tissue cultured seedling, cultivates 15 ~ 25 days.
CN201610011872.7A 2016-01-08 2016-01-08 Promote fungal bacterial strain and its application of rhodiola accumulation rhodioside Expired - Fee Related CN105543105B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201610011872.7A CN105543105B (en) 2016-01-08 2016-01-08 Promote fungal bacterial strain and its application of rhodiola accumulation rhodioside

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201610011872.7A CN105543105B (en) 2016-01-08 2016-01-08 Promote fungal bacterial strain and its application of rhodiola accumulation rhodioside

Publications (2)

Publication Number Publication Date
CN105543105A true CN105543105A (en) 2016-05-04
CN105543105B CN105543105B (en) 2018-10-16

Family

ID=55822664

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201610011872.7A Expired - Fee Related CN105543105B (en) 2016-01-08 2016-01-08 Promote fungal bacterial strain and its application of rhodiola accumulation rhodioside

Country Status (1)

Country Link
CN (1) CN105543105B (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109207375A (en) * 2018-10-09 2019-01-15 山西大学 A kind of method for preserving of plant endogenesis epiphyte strain
CN109797107A (en) * 2019-01-29 2019-05-24 西北大学 A kind of preparation method of hypha,hyphae fine powder
CN114891639A (en) * 2021-11-23 2022-08-12 河北省科学院生物研究所 Microbial strain for improving salidroside content and fermentation method thereof

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104212725A (en) * 2014-09-15 2014-12-17 山西大学 Method for improving content of salidroside and tyrosol in rhodiola rosea tissue culture seedling
CN104388497A (en) * 2014-12-01 2015-03-04 山西大学 Method for producing salidroside and tyrosol employing phialocephala fortinii

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104212725A (en) * 2014-09-15 2014-12-17 山西大学 Method for improving content of salidroside and tyrosol in rhodiola rosea tissue culture seedling
CN104388497A (en) * 2014-12-01 2015-03-04 山西大学 Method for producing salidroside and tyrosol employing phialocephala fortinii

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
CUI JL 等: "Fungal endophyte-induced salidroside and tyrosol biosynthesis combined with signal cross-talk and the mechanism of enzyme gene expression in Rhodiola crenulata", 《SCIENTIFIC REPORTS》 *
焦晋: "内生真菌对红景天次生代谢产物积累的调控及信号转导研究", 《中国》 *
王梦亮 等: "微生物催化D-葡萄糖与酪醇葡糖基转移合成红景天甙的初步研究", 《催化学报》 *

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109207375A (en) * 2018-10-09 2019-01-15 山西大学 A kind of method for preserving of plant endogenesis epiphyte strain
CN109797107A (en) * 2019-01-29 2019-05-24 西北大学 A kind of preparation method of hypha,hyphae fine powder
CN109797107B (en) * 2019-01-29 2021-06-04 西北大学 Preparation method of fungus mycelium fine powder
CN114891639A (en) * 2021-11-23 2022-08-12 河北省科学院生物研究所 Microbial strain for improving salidroside content and fermentation method thereof

Also Published As

Publication number Publication date
CN105543105B (en) 2018-10-16

Similar Documents

Publication Publication Date Title
CN101392227B (en) Bacillus prodigiosus and prodigiosin produced thereby
CN109593681B (en) Composite microbial inoculum for preventing and treating cyanobacterial bloom in aquaculture and preparation method and application thereof
CN104164367B (en) Dried silkworm cordyceps militaris and culture method thereof
CN104212725B (en) Improve rhodioside and the method for butyl alcohol content in Radix Rhodiolae tissue cultured seedling
CN105494715B (en) A kind of technique of inocalation method production dark green tea
CN106497806A (en) A kind of coronoid process dissipate capsule bacterium strain and its application
CN100510061C (en) Method for raising quality of protocorm of Tiepi stem of noble dendrobium cultivated in liquid through inductor of mycorrhizal fungi
CN104871821A (en) Phellinus igniarius strain and culturing method for same
CN105193905A (en) Traditional Chinese medicine processing technology of ganoderma-astragalus membranaceus bidirectional fermentation
CN103756911A (en) Hypocrea virens and its application
CN101248799B (en) Verticillium lecanii pesticides and uses thereof
CN103865800B (en) The Ramulus et folium taxi cuspidatae endogenetic fungus of baccatin III is produced in one strain
CN108676755A (en) A kind of microbial liquid fertilizer and its preparation method and application containing bacillus
CN109706084A (en) A kind of Radix Salviae Miltiorrhizae endogenetic fungus and its application in promotion Salvia miltiorrhiza Growth/or effective component synthesis
CN103483040A (en) Culture medium for large-scale submerged fermentation for cordyceps sinensis and fermentation production method thereof
CN105543105B (en) Promote fungal bacterial strain and its application of rhodiola accumulation rhodioside
CN104250624B (en) A kind of preparation method of the active flora of HyM soil remediations
CN104087645A (en) Solid fermentation ginseng flower and preparation method thereof
CN114032190A (en) Lactobacillus reuteri capable of fermenting dendrobium and effectively repairing solar dermatitis by fermentation liquor of dendrobium
CN103555616B (en) One bacillus amyloliquefaciens and the application of liquid preparation in control walnut root rot thereof
CN103966139B (en) A kind of short lactobacillus of highly producing gamma-aminobutyric acid in Pickles, Sichuan Style
CN100412186C (en) Tinder fungus and process for deep liquid fermentation preparation of tinder fungus
CN101407767A (en) Method for producing Chinese caterpillar fungus by fermentation
CN100457891C (en) New method for cultivating umbellate pore fungus mycelium to form umbellate pore fungus sclerotium
CN106748456A (en) The anti-corn stalk rot disease organic fertilizer and preparation method prepared as raw material with Chinese medicine dreg

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant
CF01 Termination of patent right due to non-payment of annual fee
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20181016

Termination date: 20220108