CN110755344A - Ganoderma lucidum-rhizoma polygonati bidirectional fermentation process and composition - Google Patents
Ganoderma lucidum-rhizoma polygonati bidirectional fermentation process and composition Download PDFInfo
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Abstract
The invention relates to the technical field of lucid ganoderma-polygonatum rhizome, and discloses a lucid ganoderma-polygonatum rhizome bidirectional fermentation composition, which comprises the following raw materials: the method comprises the following raw materials: 200.13 to 0.23 percent of distilled water U, 0.4 to 0.5 percent of 10 percent NaOH, 20 to 30 percent of lucid ganoderma-polygonatum odoratum/polygonatum sibiricum fermentation liquid compound liquid, 0.2 to 1.2 percent of carnation ketone, 0.03 to 0.08 percent of xanthan gum, and a lucid ganoderma-polygonatum sibiricum bidirectional fermentation process, which comprises the following steps: s1: the optimal bidirectional fermentation process of ganoderma lucidum and polygonatum sibiricum. According to the ganoderma-polygonatum bidirectional fermentation process and the composition, ganoderma and polygonatum are fermented by a bidirectional fermentation technology, the DPPH removing capacity of ganoderma-polygonatum bidirectional fermentation liquid is obviously superior to that of polygonatum stock solution, the ganoderma-polygonatum bidirectional fermentation liquid can be used as a potential cosmetic anti-aging raw material, the ganoderma-polygonatum bidirectional fermentation liquid has an obvious effect on inhibiting hyaluronidase, can be developed and used as a potential anti-inflammation or anti-acne cosmetic raw material, and the application of the ganoderma-polygonatum bidirectional fermentation liquid in a mask has the effects of moisturizing, moisturizing and whitening.
Description
Technical Field
The invention relates to the technical field of lucid ganoderma-polygonatum rhizome, in particular to a lucid ganoderma-polygonatum rhizome bidirectional fermentation process and a composition.
Background
The bidirectional fermentation technology is a mode of fermenting by using fungi as fermentation strains and using medicinal materials or dregs as medicinal substrates to jointly form a fermentation combination. Compared with the original medicinal materials, the mycoplasm after bidirectional fermentation mainly has effects in several aspects: enhance the drug effect and expand the application. The bidirectional fermentation engineering shows the organic combination of medicinal fungi (containing other fungi) and Chinese medicinal materials, and well overcomes the problem of utilization of wild medicinal plants. The rhizoma Polygonati extract has low polyphenol content, low DPPH removal rate, and poor efficacy. The rhizoma Polygonati polysaccharide obtained from rhizoma Polygonati extract has high molecular weight and is not easy to be absorbed by skin.
Ganoderma lucidum (Ganoderma lucidum) is a precious Chinese medicinal material called 'strengthening body resistance and consolidating constitution, nourishing and strengthening', belongs to the medical fungus of Polyporaceae, and has been used for treating diseases such as liver disease, high cholesterol, hyperlipemia, etc. The main antioxidant active ingredients in ganoderma are considered ganoderma triterpene and ganoderma polysaccharide. The ganoderan can also significantly promote DNA synthesis in nucleus, promote cell division, delay aging, and enhance immunity. The ganoderma lucidum is divided into three growth stages: spores, hypha, sporocarp and ganoderma lucidum are taken as a second growth stage, in recent years, the ganoderma lucidum mycelium and fermentation liquor are fully utilized, and a new application approach is developed for ganoderma lucidum.
Rhizoma Polygonati (Polygonatum sibiricum) is a rare Chinese herbal medicine in China, is a dry tuber of various plants in Polygonatum of Liliaceae, has mild nature and sweet taste, enters lung, spleen and kidney channels, and has the effects of invigorating qi, nourishing yin, moistening lung, tonifying spleen, tonifying kidney and the like. Rhizoma Polygonati contains various chemical components including saccharide, protein, vitamins, saponin, anthraquinone compounds, alkaloid, lignan and various amino acids beneficial to human body. The health-care tea is a high-quality resource integrating nutrition, health-care and medicinal functions, has a long clinical medicinal history, and has the effects of improving immunity, reducing blood sugar, reducing blood fat, resisting tumors, resisting bacteria and viruses, enhancing immunity, delaying senescence and the like as proved by modern pharmacological tests. Sealwort is an important medicinal and edible Chinese medicinal material in China, so the application of sealwort in functional raw materials of medicines, health-care foods and cosmetics is highly valued by researchers, doctors, health preserving nationalities and cosmetic engineers.
There are about 40 thousands of natural fungi, wherein thousands of high-grade fungi, hundreds of fungi for medical use and food use at present, the enzymes of known fungi are nearly 400, the enzyme systems of different fungi are different, and about ten thousand different components of traditional Chinese medicinal materials are different. If the two parties are combined in a crossed way, a large number of different fermentation combinations can be formed, the mycoplasm produced by the different fermentation combinations, which are almost unlimited and have different components and properties, respectively has certain change characteristics, can be screened by using corresponding indexes such as immunity, drug effect and the like according to established clinical treatment targets, and can be obtained into fermentation products with certain efficacy, and then selected varieties are further researched to develop into novel fungus products.
The invention discloses a composition obtained by bidirectional fermentation technology of ganoderma lucidum and polygonatum officinale, and develops application of the composition in the field of cosmetics.
Disclosure of Invention
Technical problem to be solved
Aiming at the defects of the prior art, the invention provides a ganoderma-polygonatum bidirectional fermentation process and a composition, which have the advantages of application in the field of cosmetics and the like, and solve the problem that bidirectional fermentation products of ganoderma and polygonatum officinale are not applied to the cosmetic industry through literature and patent research.
(II) technical scheme
In order to achieve the purpose of the application in the field of cosmetics, the invention provides the following technical scheme: a ganoderma-polygonatum bidirectional fermentation composition comprises the following raw materials: 200.13 to 0.23 percent of distilled water U, 0.4 to 0.5 percent of 10 percent NaOH, 20 to 30 percent of lucid ganoderma, polygonatum odoratum and sealwort fermentation liquor compound liquid, 0.2 to 1.2 percent of fragrant fresh ketone and 0.03 to 0.08 percent of xanthan gum.
Preferably, the lucid ganoderma and polygonatum odoratum compound liquid is prepared by the following method: respectively weighing 1, 3-butanediol and glycerol at a mass ratio of 1:1, uniformly mixing, preparing into polyol, respectively weighing polyol and lucid ganoderma and polygonatum odoratum fermentation liquor at a mass ratio of 3:7, and uniformly mixing.
Preferably, the polygonatum sibiricum fermentation liquor compound liquid is prepared by the following method: respectively weighing 1, 3-butanediol and glycerol in a mass ratio of 1:1, uniformly mixing, and preparing into polyol; respectively weighing polyalcohol and ganoderma lucidum and rhizoma polygonati fermentation liquor according to the mass ratio of 3:7, and uniformly mixing.
A bidirectional fermentation process of Ganoderma lucidum-rhizoma Polygonati comprises the following steps:
s1: a liquid culture medium suitable for ganoderma lucidum is used as a culture medium for bidirectional fermentation, a polygonatum extract is added for bidirectional fermentation, and the concentration, the bacterial liquid inoculation amount and the fermentation pH value of the added polygonatum extract are determined through a one-factor experiment.
S2: performing enlarged culture on the ganoderma lucidum and polygonatum sibiricum by using a 5L fermentation tank.
S3: and (3) detecting the antioxidant effect, namely respectively measuring the DPPH free radical removal capacity of sealwort stock solution, lucid ganoderma-sealwort fermentation liquor, lucid ganoderma-sealwort bidirectional fermentation liquor and culture medium which is not subjected to bidirectional fermentation with different concentrations, and respectively detecting the content of total polyphenol in the lucid ganoderma-sealwort bidirectional fermentation liquor and the culture medium which is not subjected to bidirectional fermentation.
S4: and (3) detecting the anti-inflammatory effect, namely detecting the hyaluronidase inhibition capability of the bidirectional fermentation broth of the ganoderma lucidum and the polygonatum sibiricum.
S5: and (4) detecting the molecular weight of the polysaccharide.
S6: evaluating the human body moisturizing effect, and detecting the effect of the ganoderma lucidum and polygonatum sibiricum bidirectional fermentation liquid in cosmetics.
Preferably, the inoculation percentage content of the bacterial liquid in the S1 is 2% -10%, the pH value in the S1 is 5.2-9.2, and the concentration of the additive liquid of the yellow essence in the S1 is 20% -100%.
Preferably, the culture medium without bidirectional fermentation in S2: 5.0g of peptone, 10.0g of glucose, 5.0g of sodium chloride, 0.2g of calcium carbonate, 1.0L of distilled water, and pH7.2-7.4, wherein in S2, the bidirectional fermentation culture medium of ganoderma lucidum and polygonatum sibiricum: peptone 12.5g, glucose 25.0g, sodium chloride 12.5g, calcium carbonate 0.5g, distilled water 2.5L, rhizoma Polygonati powder: 50g, pH7.2-7.4, putting the prepared culture medium into a refrigerator at 4 ℃, and culturing in a ganoderma lucidum and polygonatum sibiricum fermentation tank in the same time as the bidirectional fermentation: inoculating the cultured secondary seed liquid into a 5L fermentation tank containing 2.5L culture medium in an inoculation amount of 10% (V/V), culturing at 26 deg.C and dissolved oxygen amount of 50-100% at 200r/min for 141h, and fermenting with Ganoderma and rhizoma Polygonati bidirectional fermentation liquid: culturing in fermentation tank, sterilizing at 120 deg.C under high temperature and high pressure for 30min, cooling, filtering with 0.2 μm filter plate, and adding 0.8% PEHG into the obtained fermentation broth for storage.
Preferably, in the step S3, the ganoderma lucidum and polygonatum sibiricum fermentation liquid subjected to bidirectional submerged fermentation is diluted to a dilution concentration of 1% -25%, and the culture medium not subjected to bidirectional fermentation is diluted to a dilution concentration of 25% -100%, 1mL of a sample to be tested is taken, 5mL of 10% forskolin phenol reagent is respectively added, the mixture is uniformly mixed, after 7min, 4mL of 7.5% sodium carbonate solution is added, and after 1h, the light absorption value is measured at 765 nm.
Preferably, the concentration of the bidirectional fermentation broth of ganoderma lucidum and polygonatum sibiricum in the S4 is 10-100%.
Preferably, the molecular weight of the polysaccharide in the S5 culture medium without bidirectional fermentation is 21%, and the molecular weight of the polysaccharide in the ganoderma lucidum and polygonatum sibiricum bidirectional fermentation liquor is 60%.
Preferably, in S6, after cleaning the face, the subject sits still in a constant-temperature and constant-humidity laboratory environment for 20min, and then the data of the water content, the water dispersion loss and the glossiness of the skin background are tested, the subject uses the ganoderma lucidum and polygonatum sibiricum fermentation liquor mask, the mask is removed after 15min, the situation that the absorption of the mask liquor is not easy is avoided, and the water content, the water dispersion loss, the skin chromaticity and the glossiness of the skin are respectively tested when the mask is used for 0-4h after 15 min.
Compared with the prior art, the invention provides a ganoderma-polygonatum bidirectional fermentation process and a composition, which have the following beneficial effects:
1. the ganoderma lucidum-polygonatum sibiricum bidirectional fermentation process and the composition are characterized in that ganoderma lucidum and polygonatum sibiricum are fermented by a bidirectional fermentation technology, the DPPH removing capacity of a ganoderma lucidum-polygonatum sibiricum bidirectional fermentation liquid is obviously superior to that of a polygonatum sibiricum stock solution, the DPPH removing capacity of the ganoderma lucidum-polygonatum sibiricum bidirectional fermentation liquid is superior to that of a culture medium which is not subjected to bidirectional fermentation, and the total polyphenol content of the ganoderma lucidum-polygonatum sibiricum bidirectional fermentation liquid is higher than that of the culture medium which is not subjected to bidirectional fermentation, so that the content of effective components of traditional Chinese medicines is improved through bidirectional fermentation, the curative effect is improved, the synergistic effect is achieved, the ganoderma lucidum-polygonatum sibiricum bidirectional fermentation liquid can be used as a potential cosmetic aging delaying raw material, the ganoderma lucidum-polygonatum sibiricum bidirectional fermentation liquid has an obvious effect on, Moisturizing and whitening effects.
Detailed Description
The first embodiment is as follows:
a ganoderma-polygonatum bidirectional fermentation composition comprises the following raw materials: the preparation method comprises the following steps of preparing distilled water U200.18%, 10% of NaOH0.45%, a lucid ganoderma and polygonatum odoratum/polygonatum sibiricum fermentation liquor compound liquid 25%, jasminones 0.5% and xanthan gum 0.05%, wherein the lucid ganoderma and polygonatum sibiricum compound liquid is prepared by the following method: respectively weighing 1, 3-butanediol and glycerol in a mass ratio of 1:1, uniformly mixing, preparing into polyol, respectively weighing polyol and lucid ganoderma and polygonatum odoratum fermentation liquor in a mass ratio of 3:7, uniformly mixing, and preparing the polygonatum sibiricum fermentation liquor compound liquor by the following method: respectively weighing 1, 3-butanediol and glycerol in a mass ratio of 1:1, uniformly mixing, and preparing into polyol; respectively weighing polyhydric alcohol and ganoderma lucidum and polygonatum sibiricum fermentation liquor according to the mass ratio of 3:7, uniformly mixing, weighing raw materials required for preparing 100g of facial mask liquor, adding a proper amount of distilled water into a 150mL beaker, slightly scattering U20 on the water surface without stirring, soaking U20 in water, placing the beaker in an electric ceramic furnace, heating to 85 ℃, keeping the temperature for 30min, adding menthone when cooling to 65 ℃, uniformly stirring, dropwise adding 10% NAOH after cooling to room temperature, ensuring that the ratio of U20 to 10% NAOH is 1:2.5, uniformly stirring, adding ganoderma lucidum and polygonatum sibiricum fermentation liquor compound liquid, filling the facial mask liquor into facial mask bags, and ensuring that the liquid loading amount of each facial mask is 25 g.
A bidirectional fermentation process of Ganoderma lucidum-rhizoma Polygonati comprises the following steps:
s1: a process for preparing the dual-direction fermented ganoderma-siberian solomonseal rhizome includes such steps as adding the extract of siberian solomonseal rhizome to the liquid culture medium, dual-direction fermenting, determining the concentration, inoculation quantity and pH value of the extract, changing the percentage content of inoculation to 4%, comparing the growth of ganoderma, determining the inoculation quantity, growing for 54 hr, controlling the pH value to 7.2, concentration of extract to 100%, and exploring the change rule of pH value in fermenting ganoderma, the pH value is 6.2, the growth characters of the ganoderma are observed and compared, the optimal fermentation pH is determined, according to the existing documents, the invariants are controlled to be a ganoderma growth culture medium which is verified and optimized, the inoculum size is 6%, the concentration of the polygonatum extract is 100%, the ganoderma growth culture medium grows for 54h, the ganoderma grows into large balls which are not split, the higher the pH is, the larger the spawn balls are, the growth of fungi can be inhibited due to the addition of high-concentration traditional Chinese medicines, therefore, the concentration of the polygonatum additive liquid in the bidirectional fermentation substrate needs to be determined, the concentration of the polygonatum additive liquid is changed to be 100%, and the concentration of the polygonatum additive liquid is determined by observing and comparing the growth characters of the ganoderma.
S2: performing fermentation tank amplification culture of bidirectional fermentation of ganoderma lucidum and polygonatum sibiricum, performing amplification culture on the fermentation of the ganoderma lucidum and polygonatum sibiricum by using a 5L fermentation tank, wherein a culture medium which is not subjected to bidirectional fermentation: 5.0g of peptone, 10.0g of glucose, 5.0g of sodium chloride, 0.2g of calcium carbonate, 1.0L of distilled water, pH7.2-7.4, and a bidirectional fermentation culture medium of ganoderma lucidum and polygonatum sibiricum: peptone 12.5g, glucose 25.0g, sodium chloride 12.5g, calcium carbonate 0.5g, distilled water 2.5L, rhizoma Polygonati powder: 50g, pH7.2-7.4, putting the prepared culture medium into a refrigerator at 4 ℃, and culturing in a ganoderma lucidum and polygonatum sibiricum fermentation tank in the same time as the bidirectional fermentation: inoculating the cultured secondary seed liquid into a 5L fermentation tank containing 2.5L culture medium in an inoculation amount of 10% (V/V), culturing at 26 deg.C and dissolved oxygen amount of 50-100% at 200r/min for 141h, and fermenting with Ganoderma and rhizoma Polygonati bidirectional fermentation liquid: culturing in fermentation tank, sterilizing at 120 deg.C under high temperature and high pressure for 30min, cooling, filtering with 0.2 μm filter plate, and adding 0.8% PEHG into the obtained fermentation broth for storage.
S3: detecting the antioxidant effect, namely respectively measuring the capacity of removing DPPH free radicals of sealwort stock solution, lucid ganoderma-sealwort fermentation liquor, lucid ganoderma sealwort bidirectional fermentation liquor and culture medium which is not subjected to bidirectional fermentation at different concentrations, and respectively detecting the content of total polyphenol in the lucid ganoderma sealwort bidirectional fermentation liquor and the culture medium which is not subjected to bidirectional fermentation, wherein the preparation method comprises the following steps: 0.2mM/L of DPPH ethanol solution preparation: weighing 20mgDPPH, adding absolute ethyl alcohol to dissolve, fixing the volume in a 250mL volumetric flask, and storing at 4 ℃ in a dark place; preparing 100 mu g/mL VC solution: weighing 25mgVC, adding distilled water for dissolving, dissolving in a 250mL volumetric flask, and storing in dark place; sample concentration: diluting the ganoderma lucidum and polygonatum sibiricum fermentation liquor subjected to bidirectional submerged fermentation to a dilution concentration of 10%, and diluting a culture medium which is not subjected to bidirectional fermentation to a dilution concentration of 25%; the experimental process comprises the following steps: mixing 1.0mL of the solution to be tested with 1.0mL of DPPH solution (tube A); 1.0mL of water was mixed with 1.0mL of DPPH solution (tube B); uniformly mixing 1.0mL of absolute ethyl alcohol solution with 1.0mL of solution to be detected (tube C); after reacting for 30min in the dark, measuring the absorbance value of an A, B, C tube at 517 nm; calculating the clearance rate: clearance (%) [ (B + C) -a ]/B100%; the clearance rate of fermentation liquor obtained by deep fermentation of polygonatum sibiricum powder by ganoderma lucidum on DPPH (DPPH) at different concentrations is utilized to detect the content of total polyphenol in ganoderma lucidum and polygonatum sibiricum bidirectional fermentation liquor and a culture medium which is not subjected to bidirectional fermentation: reagent preparation: 10% forinophenol reagent (ready mix): 15mL of forinophenol is added into 135mL of deionized water and is uniformly mixed to obtain the product; 7.5% sodium carbonate: weighing 15g of sodium carbonate, and fixing the volume to 200mL by using deionized water; the experimental process comprises the following steps: taking 1mL of a sample to be detected, respectively adding 5mL of 10% forskolin phenol reagent, uniformly mixing, adding 4mL of 7.5% sodium carbonate solution after 7min, and measuring the light absorption value at 765nm after 1 h; the experimental results are as follows: through experimental detection, the total polyphenol content of the two solutions to be detected is from small to large as the bidirectional fermentation broth of the lucid ganoderma and the polygonatum sibiricum > culture medium which is not subjected to bidirectional fermentation, and the total polyphenol content of the fermentation broth subjected to the bidirectional fermentation of the lucid ganoderma and the polygonatum sibiricum is higher by comparing the fermentation broth subjected to the bidirectional fermentation of the lucid ganoderma and the culture medium which is not subjected to the bidirectional fermentation.
S4: detecting anti-inflammatory efficacy, namely detecting the hyaluronidase inhibition capability of bidirectional fermentation broth of ganoderma lucidum and polygonatum sibiricum, and preparing a reagent: hyaluronidase (I): the concentration is 500U/mL, the solution can be used as it is and can not stay overnight, and acetic acid buffer solution is used as solvent; sodium hyaluronate: 0.5mg/mL, prepared once and used for many times, and acetic acid buffer is used as a solvent; buffer: 4.8mL of solution A (0.2mol/L acetic acid 11.55mL glacial acetic acid dissolved in 1L distilled water); 45.2mL of solution B (0.2mol/L of sodium acetate 16.4g of anhydrous sodium acetate or 27.2g of sodium acetate trihydrate dissolved in 1L of distilled water) is mixed and diluted to 100mL to prepare an acetic acid buffer solution with the pH value of 5.6; acetylacetone solution: 50mL of 1.0mol/L sodium carbonate solution and 3.5mL of acetylacetone solution are mixed uniformly (prepared immediately before use); P-DAB color developing agent: dissolving 0.8g of p-dimethylaminobenzaldehyde in 15mL of concentrated hydrochloric acid and 15mL of absolute ethyl alcohol, and uniformly mixing; calcium chloride solution CaCl 2: 2.5 mol/L; sodium hydroxide solution NaOH: 5mol/L, the inhibition rate of the ganoderma lucidum and polygonatum sibiricum bidirectional fermentation broth on hyaluronidase increases along with the increase of the concentration of the ganoderma lucidum and polygonatum sibiricum bidirectional fermentation broth, iridin has an obvious inhibition effect on hyaluronidase, the inhibition rate reaches up to 82.3 percent, and the ganoderma lucidum and polygonatum sibiricum bidirectional fermentation broth has a good in-vitro anti-inflammatory effect.
S5: detecting the molecular weight of polysaccharide, and treating a sample: removing protein by adopting a sevage method, taking 5mL of a sample, adding 1mL of a prepared sevage reagent, oscillating, centrifuging, taking out an upper layer solution, and repeating the operation until no white substance appears in the middle; and (3) separation and purification of polysaccharide: pre-treating the gel, loading into a chromatographic column with the specification of 50 × 1.6cm and the flow rate of a pump of 0.5mL/min, and pumping a mobile phase to balance the chromatographic column; after the column is balanced, carrying out sample loading operation, taking samples to be separated, respectively preparing the samples into polysaccharide solutions of 5mg/mL, wherein the sample loading volume is 4mL, ultrapure water is used as an elution solvent, the flow rate is 1mL/min, collecting one tube every 10min, and collecting eluent; and (3) detecting the purity of the fraction: detecting the purity of the sample by adopting a gel permeation chromatography-eighteen-angle laser scattering combined system (GPC-MALS system), and measuring the purified sample by a GPC-MALS method through a water system filter head with the diameter of 0.22 mu m; a transfusion system: waters e 2695 system; gel chromatography column: shodex SUGER KS-805/KS-803; a detector: the eighteen-angle laser light scattering instrument is used together with a differential detector; sample introduction volume: 0.1 mL; flow rate: 1 mL/min; column temperature: 60 ℃; refractive index detector temperature: 50 ℃; mobile phase: a mixed solution of 0.1mol/L sodium nitrate solution and 0.02% sodium azide solution (mobile phase is filtered by a 0.22 mu m water system filter membrane in low vacuum degree); the experimental result shows that the molecular weight of the polysaccharide without the bidirectional fermentation culture medium is 21 percent, and the molecular weight of the polysaccharide of the bidirectional fermentation broth of the ganoderma lucidum and the polygonatum sibiricum is 60 percent.
S6: evaluating the human body moisturizing effect, and detecting the effect of the ganoderma lucidum and polygonatum sibiricum bidirectional fermentation liquid in cosmetics; after a subject cleans the face, the subject sits statically for 20min in a constant-temperature constant-humidity laboratory environment, data of water content, water dispersion loss and glossiness of the skin background are tested, the subject uses the lucid ganoderma and polygonatum sibiricum fermentation liquor mask, the mask is uncovered after 15min and is easy to absorb by the mask liquid, the moisture content value, the moisture dispersion loss, the skin chromaticity and the glossiness of the skin are respectively detected in time, 0.5h, 2h, 3h and 4h after the mask is used for 15min, and the experimental result shows that the lucid ganoderma and polygonatum sibiricum mask has a remarkable effect on improving the moisture content of the skin, the moisture content of the skin in time when the mask is used is improved from 46 to 72, and the moisture content of the skin is still higher than the moisture content of the skin when the mask is not used for 4h, so that the lucid ganoderma and polygonatum sibiricum fermentation liquor mask has a certain; after the mask with the lucid ganoderma and polygonatum sibiricum fermentation liquor is used, the water dispersion loss of the skin is slightly reduced, and the rising trend is avoided, so that a certain moisturizing effect is achieved; the skin glossiness of the ganoderma lucidum and polygonatum sibiricum fermentation liquor mask is improved before and after use; the lucid ganoderma and polygonatum sibiricum fermentation liquor mask can improve the skin whiteness within 4 hours; the ganoderma lucidum and polygonatum sibiricum fermentation liquor mask has a certain effect on reducing skin erythema; the effect of the ganoderma lucidum and rhizoma polygonati fermentation liquor mask on removing yellow is not obvious.
Example two:
a ganoderma-polygonatum bidirectional fermentation composition comprises the following raw materials: the preparation method comprises the following steps of preparing distilled water U200.19%, 10% of NaOH0.46%, a lucid ganoderma and polygonatum odoratum/polygonatum sibiricum fermentation liquor compound liquid 27%, jasminon 0.6% and xanthan gum 0.07%, wherein the lucid ganoderma and polygonatum sibiricum compound liquid is prepared by the following method: respectively weighing 1, 3-butanediol and glycerol in a mass ratio of 1:1, uniformly mixing, preparing into polyol, respectively weighing polyol and lucid ganoderma and polygonatum odoratum fermentation liquor in a mass ratio of 3:7, uniformly mixing, and preparing the polygonatum sibiricum fermentation liquor compound liquor by the following method: respectively weighing 1, 3-butanediol and glycerol in a mass ratio of 1:1, uniformly mixing, and preparing into polyol; respectively weighing polyhydric alcohol and ganoderma lucidum and polygonatum sibiricum fermentation liquor according to the mass ratio of 3:7, uniformly mixing, weighing raw materials required for preparing 100g of facial mask liquor, adding a proper amount of distilled water into a 150mL beaker, slightly scattering U20 on the water surface without stirring, soaking U20 in water, placing the beaker in an electric ceramic furnace, heating to 85 ℃, keeping the temperature for 30min, adding menthone when cooling to 65 ℃, uniformly stirring, dropwise adding 10% NAOH after cooling to room temperature, ensuring that the ratio of U20 to 10% NAOH is 1:2.5, uniformly stirring, adding ganoderma lucidum and polygonatum sibiricum fermentation liquor compound liquid, filling the facial mask liquor into facial mask bags, and ensuring that the liquid loading amount of each facial mask is 25 g.
A bidirectional fermentation process of Ganoderma lucidum-rhizoma Polygonati comprises the following steps:
s1: a process for preparing the optimal bidirectional fermenting liquid culture medium of ganoderma includes such steps as adding the liquid culture medium suitable for ganoderma to obtain the culture medium, bidirectional fermenting, determining the concentration, inoculation amount and pH value of the liquid culture medium, changing the percentage of inoculation to 8%, comparing the growth of ganoderma, determining the inoculation amount of liquid culture medium, growing for 54 hr, controlling the pH value to 7.2 and 100%, determining the pH value to greatly influence the metabolism of microbes, and exploring the change rule of pH value in fermenting ganoderma, the pH value is 8.2, the growth characteristics of the ganoderma are observed and compared, the optimal fermentation pH is determined, according to the existing documents, the invariants are controlled to be a ganoderma growth culture medium which is verified and optimized, the inoculum size is 6%, the concentration of rhizoma polygonati extract is 100%, the ganoderma growth culture medium grows for 54h, the ganoderma grows into large balls which are not split, the higher the pH is, the larger the bacterial balls are, the conventional pH7.2 of the documents is determined, and the growth of fungi is inhibited due to the addition of high-concentration traditional Chinese medicines, so that the concentration of the additive solution of the polygonatum is necessarily determined, the concentration of the additive solution of the polygonatum is changed to 80%, and the concentration of the additive solution of the polygonatum is determined by observing and comparing the growth characteristics of the ganoderma.
S2: performing fermentation tank amplification culture of bidirectional fermentation of ganoderma lucidum and polygonatum sibiricum, performing amplification culture on the fermentation of the ganoderma lucidum and polygonatum sibiricum by using a 5L fermentation tank, wherein a culture medium which is not subjected to bidirectional fermentation: 5.0g of peptone, 10.0g of glucose, 5.0g of sodium chloride, 0.2g of calcium carbonate, 1.0L of distilled water, pH7.2-7.4, and a bidirectional fermentation culture medium of ganoderma lucidum and polygonatum sibiricum: peptone 12.5g, glucose 25.0g, sodium chloride 12.5g, calcium carbonate 0.5g, distilled water 2.5L, rhizoma Polygonati powder: 50g, pH7.2-7.4, putting the prepared culture medium into a refrigerator at 4 ℃, and culturing in a ganoderma lucidum and polygonatum sibiricum fermentation tank in the same time as the bidirectional fermentation: inoculating the cultured secondary seed liquid into a 5L fermentation tank containing 2.5L culture medium in an inoculation amount of 10% (V/V), culturing at 26 deg.C and dissolved oxygen amount of 50-100% at 200r/min for 141h, and fermenting with Ganoderma and rhizoma Polygonati bidirectional fermentation liquid: culturing in fermentation tank, sterilizing at 120 deg.C under high temperature and high pressure for 30min, cooling, filtering with 0.2 μm filter plate, and adding 0.8% PEHG into the obtained fermentation broth for storage.
S3: detecting the antioxidant effect, namely respectively measuring the capacity of removing DPPH free radicals of sealwort stock solution, lucid ganoderma-sealwort fermentation liquor, lucid ganoderma sealwort bidirectional fermentation liquor and culture medium which is not subjected to bidirectional fermentation at different concentrations, and respectively detecting the content of total polyphenol in the lucid ganoderma sealwort bidirectional fermentation liquor and the culture medium which is not subjected to bidirectional fermentation, wherein the preparation method comprises the following steps: 0.2mM/L of DPPH ethanol solution preparation: weighing 20mgDPPH, adding absolute ethyl alcohol to dissolve, fixing the volume in a 250mL volumetric flask, and storing at 4 ℃ in a dark place; preparing 100 mu g/mL VC solution: weighing 25mgVC, adding distilled water for dissolving, dissolving in a 250mL volumetric flask, and storing in dark place; sample concentration: diluting the fermentation liquor of ganoderma lucidum and polygonatum sibiricum obtained by bidirectional submerged fermentation with the dilution concentration of 20%, and diluting the culture medium which is not subjected to bidirectional fermentation with the dilution concentration of 25%; the experimental process comprises the following steps: mixing 1.0mL of the solution to be tested with 1.0mL of DPPH solution (tube A); 1.0mL of water was mixed with 1.0mL of DPPH solution (tube B); uniformly mixing 1.0mL of absolute ethyl alcohol solution with 1.0mL of solution to be detected (tube C); after reacting for 30min in the dark, measuring the absorbance value of an A, B, C tube at 517 nm; calculating the clearance rate: clearance (%) [ (B + C) -a ]/B100%; the clearance rate of fermentation liquor obtained by deep fermentation of polygonatum sibiricum powder by ganoderma lucidum on DPPH (DPPH) at different concentrations is utilized to detect the content of total polyphenol in ganoderma lucidum and polygonatum sibiricum bidirectional fermentation liquor and a culture medium which is not subjected to bidirectional fermentation: reagent preparation: 10% forinophenol reagent (ready mix): 15mL of forinophenol is added into 135mL of deionized water and is uniformly mixed to obtain the product; 7.5% sodium carbonate: weighing 15g of sodium carbonate, and fixing the volume to 200mL by using deionized water; the experimental process comprises the following steps: taking 1mL of a sample to be detected, respectively adding 5mL of 10% forskolin phenol reagent, uniformly mixing, adding 4mL of 7.5% sodium carbonate solution after 7min, and measuring the light absorption value at 765nm after 1 h; the experimental results are as follows: through experimental detection, the total polyphenol content of the two solutions to be detected is from small to large as the bidirectional fermentation broth of the lucid ganoderma and the polygonatum sibiricum > culture medium which is not subjected to bidirectional fermentation, and the total polyphenol content of the fermentation broth subjected to the bidirectional fermentation of the lucid ganoderma and the polygonatum sibiricum is higher by comparing the fermentation broth subjected to the bidirectional fermentation of the lucid ganoderma and the culture medium which is not subjected to the bidirectional fermentation.
S4: detecting anti-inflammatory efficacy, namely detecting the hyaluronidase inhibition capability of bidirectional fermentation broth of ganoderma lucidum and polygonatum sibiricum, and preparing a reagent: hyaluronidase (I): the concentration is 500U/mL, the solution can be used as it is and can not stay overnight, and acetic acid buffer solution is used as solvent; sodium hyaluronate: 0.5mg/mL, prepared once and used for many times, and acetic acid buffer is used as a solvent; buffer: 4.8mL of solution A (0.2mol/L acetic acid 11.55mL glacial acetic acid dissolved in 1L distilled water); 45.2mL of solution B (0.2mol/L of sodium acetate 16.4g of anhydrous sodium acetate or 27.2g of sodium acetate trihydrate dissolved in 1L of distilled water) is mixed and diluted to 100mL to prepare an acetic acid buffer solution with the pH value of 5.6; acetylacetone solution: 50mL of 1.0mol/L sodium carbonate solution and 3.5mL of acetylacetone solution are mixed uniformly (prepared immediately before use); P-DAB color developing agent: dissolving 0.8g of p-dimethylaminobenzaldehyde in 15mL of concentrated hydrochloric acid and 15mL of absolute ethyl alcohol, and uniformly mixing; calcium chloride solution CaCl 2: 2.5 mol/L; sodium hydroxide solution NaOH: 5mol/L, the inhibition rate of the ganoderma lucidum and polygonatum sibiricum bidirectional fermentation broth on hyaluronidase increases along with the increase of the concentration of the ganoderma lucidum and polygonatum sibiricum bidirectional fermentation broth, iridin has an obvious inhibition effect on hyaluronidase, the inhibition rate reaches up to 82.3 percent, and the ganoderma lucidum and polygonatum sibiricum bidirectional fermentation broth has a good in-vitro anti-inflammatory effect.
S5: detecting the molecular weight of polysaccharide, and treating a sample: removing protein by adopting a sevage method, taking 5mL of a sample, adding 1mL of a prepared sevage reagent, oscillating, centrifuging, taking out an upper layer solution, and repeating the operation until no white substance appears in the middle; and (3) separation and purification of polysaccharide: pre-treating the gel, loading into a chromatographic column with the specification of 50 × 1.6cm and the flow rate of a pump of 0.5mL/min, and pumping a mobile phase to balance the chromatographic column; after the column is balanced, carrying out sample loading operation, taking samples to be separated, respectively preparing the samples into polysaccharide solutions of 5mg/mL, wherein the sample loading volume is 4mL, ultrapure water is used as an elution solvent, the flow rate is 1mL/min, collecting one tube every 10min, and collecting eluent; and (3) detecting the purity of the fraction: detecting the purity of the sample by adopting a gel permeation chromatography-eighteen-angle laser scattering combined system (GPC-MALS system), and measuring the purified sample by a GPC-MALS method through a water system filter head with the diameter of 0.22 mu m; a transfusion system: waters e 2695 system; gel chromatography column: shodex SUGER KS-805/KS-803; a detector: the eighteen-angle laser light scattering instrument is used together with a differential detector; sample introduction volume: 0.1 mL; flow rate: 1 mL/min; column temperature: 60 ℃; refractive index detector temperature: 50 ℃; mobile phase: a mixed solution of 0.1mol/L sodium nitrate solution and 0.02% sodium azide solution (mobile phase is filtered by a 0.22 mu m water system filter membrane in low vacuum degree); the experimental result shows that the molecular weight of the polysaccharide without the bidirectional fermentation culture medium is 21 percent, and the molecular weight of the polysaccharide of the bidirectional fermentation broth of the ganoderma lucidum and the polygonatum sibiricum is 60 percent.
S6: evaluating the human body moisturizing effect, and detecting the effect of the ganoderma lucidum and polygonatum sibiricum bidirectional fermentation liquid in cosmetics; after a subject cleans the face, the subject sits statically for 20min in a constant-temperature constant-humidity laboratory environment, data of water content, water dispersion loss and glossiness of the skin background are tested, the subject uses the lucid ganoderma and polygonatum sibiricum fermentation liquor mask, the mask is uncovered after 15min and is easy to absorb by the mask liquid, the moisture content value, the moisture dispersion loss, the skin chromaticity and the glossiness of the skin are respectively detected in time, 0.5h, 2h, 3h and 4h after the mask is used for 15min, and the experimental result shows that the lucid ganoderma and polygonatum sibiricum mask has a remarkable effect on improving the moisture content of the skin, the moisture content of the skin in time when the mask is used is improved from 46 to 72, and the moisture content of the skin is still higher than the moisture content of the skin when the mask is not used for 4h, so that the lucid ganoderma and polygonatum sibiricum fermentation liquor mask has a certain; after the mask with the lucid ganoderma and polygonatum sibiricum fermentation liquor is used, the water dispersion loss of the skin is slightly reduced, and the rising trend is avoided, so that a certain moisturizing effect is achieved; the skin glossiness of the ganoderma lucidum and polygonatum sibiricum fermentation liquor mask is improved before and after use; the lucid ganoderma and polygonatum sibiricum fermentation liquor mask can improve the skin whiteness within 4 hours; the ganoderma lucidum and polygonatum sibiricum fermentation liquor mask has a certain effect on reducing skin erythema; the effect of the ganoderma lucidum and rhizoma polygonati fermentation liquor mask on removing yellow is not obvious.
Example three:
a ganoderma-polygonatum bidirectional fermentation composition comprises the following raw materials: the preparation method comprises the following steps of preparing distilled water U200.17%, 10% of NaOH0.43%, a lucid ganoderma and polygonatum odoratum/polygonatum sibiricum fermentation liquor compound liquid 24%, jasminones 0.3% and xanthan gum 0.03%, wherein the lucid ganoderma and polygonatum sibiricum compound liquid is prepared by the following method: respectively weighing 1, 3-butanediol and glycerol in a mass ratio of 1:1, uniformly mixing, preparing into polyol, respectively weighing polyol and lucid ganoderma and polygonatum odoratum fermentation liquor in a mass ratio of 3:7, uniformly mixing, and preparing the polygonatum sibiricum fermentation liquor compound liquor by the following method: respectively weighing 1, 3-butanediol and glycerol in a mass ratio of 1:1, uniformly mixing, and preparing into polyol; respectively weighing polyhydric alcohol and ganoderma lucidum and polygonatum sibiricum fermentation liquor according to the mass ratio of 3:7, uniformly mixing, weighing raw materials required for preparing 100g of facial mask liquor, adding a proper amount of distilled water into a 150mL beaker, slightly scattering U20 on the water surface without stirring, soaking U20 in water, placing the beaker in an electric ceramic furnace, heating to 85 ℃, keeping the temperature for 30min, adding menthone when cooling to 65 ℃, uniformly stirring, dropwise adding 10% NAOH after cooling to room temperature, ensuring that the ratio of U20 to 10% NAOH is 1:2.5, uniformly stirring, adding ganoderma lucidum and polygonatum sibiricum fermentation liquor compound liquid, filling the facial mask liquor into facial mask bags, and ensuring that the liquid loading amount of each facial mask is 25 g.
A bidirectional fermentation process of Ganoderma lucidum-rhizoma Polygonati comprises the following steps:
s1: a process for preparing the optimal bidirectional fermenting liquid culture medium of ganoderma includes such steps as adding the liquid culture medium suitable for ganoderma to obtain the culture medium, bidirectional fermenting, determining the concentration, inoculation amount and pH value of the liquid culture medium, changing the percentage of inoculation to 6%, comparing the growth of ganoderma, determining the inoculation amount of liquid culture medium, growing for 54 hr, controlling the pH value to 7.2 and 100%, determining the pH value to greatly influence the metabolism of microbes, and exploring the change rule of pH value in fermenting ganoderma, the pH value is 7.2, the growth characteristics of the ganoderma are observed and compared to determine the optimal fermentation pH, according to the existing literature, the invariables are controlled to be a ganoderma growth culture medium which is verified and optimized, the inoculation amount of the fungus liquid is 6 percent, the concentration of the polygonatum extract is 100 percent, the ganoderma grows for 54 hours in the optimal growth time of the ganoderma, the ganoderma grows into large balls which are not split, the higher the pH is, the larger the fungus balls are, the growth of the fungus can be inhibited due to the addition amount of high-concentration traditional Chinese medicines, therefore, the concentration of the polygonatum concentrate additive liquid in the bidirectional fermentation substrate needs to be determined, the concentration of the polygonatum additive liquid is changed to be 20 percent, the concentration of the polygonatum additive liquid is determined by observing and comparing the growth characteristics of the ganoderma, and according to the existing literature, the invariables are controlled to be the ganoderma growth culture medium which is verified and optimized, the inoculation amount of the fungus, The pH value is 7.2, the ganoderma lucidum grows for 54h according to the optimal growth time of the ganoderma lucidum, the mycelium of the polygonatum sibiricum extracting solution with the concentration of 20% is more, small and uniform, the batch is optimal, therefore, the polygonatum sibiricum extracting solution with the concentration of 20% is selected for repeated experiments, and the optimized culture medium comprises the following components: 0.5g of peptone, 1.0g of glucose, 0.5g of NaCl, 30.02g of CaCO, 6mL of bacterial liquid inoculation amount, 20% of sealwort stock solution and 7.2-7.4 of pHs.
S2: performing fermentation tank amplification culture of bidirectional fermentation of ganoderma lucidum and polygonatum sibiricum, performing amplification culture on the fermentation of the ganoderma lucidum and polygonatum sibiricum by using a 5L fermentation tank, wherein a culture medium which is not subjected to bidirectional fermentation: 5.0g of peptone, 10.0g of glucose, 5.0g of sodium chloride, 0.2g of calcium carbonate, 1.0L of distilled water, pH7.2-7.4, and a bidirectional fermentation culture medium of ganoderma lucidum and polygonatum sibiricum: peptone 12.5g, glucose 25.0g, sodium chloride 12.5g, calcium carbonate 0.5g, distilled water 2.5L, rhizoma Polygonati powder: 50g, pH7.2-7.4, putting the prepared culture medium into a refrigerator at 4 ℃, and culturing in a ganoderma lucidum and polygonatum sibiricum fermentation tank in the same time as the bidirectional fermentation: inoculating the cultured secondary seed liquid into a 5L fermentation tank containing 2.5L culture medium in an inoculation amount of 10% (V/V), culturing at 26 deg.C and dissolved oxygen amount of 50-100% at 200r/min for 141h, and fermenting with Ganoderma and rhizoma Polygonati bidirectional fermentation liquid: culturing in fermentation tank, sterilizing at 120 deg.C under high temperature and high pressure for 30min, cooling, filtering with 0.2 μm filter plate, and adding 0.8% PEHG into the obtained fermentation broth for storage.
S3: detecting the antioxidant effect, namely respectively measuring the capacity of removing DPPH free radicals of sealwort stock solution, lucid ganoderma-sealwort fermentation liquor, lucid ganoderma sealwort bidirectional fermentation liquor and culture medium which is not subjected to bidirectional fermentation at different concentrations, and respectively detecting the content of total polyphenol in the lucid ganoderma sealwort bidirectional fermentation liquor and the culture medium which is not subjected to bidirectional fermentation, wherein the preparation method comprises the following steps: 0.2mM/L of DPPH ethanol solution preparation: weighing 20mgDPPH, adding absolute ethyl alcohol to dissolve, fixing the volume in a 250mL volumetric flask, and storing at 4 ℃ in a dark place; preparing 100 mu g/mL VC solution: weighing 25mgVC, adding distilled water for dissolving, dissolving in a 250mL volumetric flask, and storing in dark place; sample concentration: diluting the ganoderma lucidum and polygonatum sibiricum fermentation liquor subjected to bidirectional submerged fermentation to the dilution concentration of 11.63%, and diluting the culture medium which is not subjected to bidirectional fermentation to the dilution concentration of 66.16%; the experimental process comprises the following steps: mixing 1.0mL of the solution to be tested with 1.0mL of DPPH solution (tube A); 1.0mL of water was mixed with 1.0mL of DPPH solution (tube B); uniformly mixing 1.0mL of absolute ethyl alcohol solution with 1.0mL of solution to be detected (tube C); after reacting for 30min in the dark, measuring the absorbance value of an A, B, C tube at 517 nm; calculating the clearance rate: clearance (%) [ (B + C) -a ]/B100%; the method comprises the following steps of utilizing ganoderma lucidum to carry out submerged fermentation on sealwort powder to obtain fermentation liquor, wherein the clearance rate of the fermentation liquor to DPPH (dehydroepiandrosterone) is different concentrations, when the clearance rate of DPPH is calculated to be 50%, the concentration of the fermentation liquor is 11.63%, the clearance rate of a culture medium which is not subjected to bidirectional fermentation to DPPH is different concentrations, when the clearance rate of DPPH is calculated to be 50%, the concentration of the fermentation liquor is 66.16%, when the clearance rate of DPPH is 50%, the concentration of the required fermentation liquor of ganoderma lucidum and sealwort is 11.63%, the concentration of the culture medium which is not subjected to bidirectional fermentation is 66.16%, the antioxidant capacity of the fermentation of ganoderma lucidum and sealwort is higher than that of the culture medium which is not subjected to bidirectional fermentation, and detecting the content of total polyphenols in the: reagent preparation: 10% forinophenol reagent (ready mix): 15mL of forinophenol is added into 135mL of deionized water and is uniformly mixed to obtain the product; 7.5% sodium carbonate: weighing 15g of sodium carbonate, and fixing the volume to 200mL by using deionized water; the experimental process comprises the following steps: taking 1mL of a sample to be detected, respectively adding 5mL of 10% forskolin phenol reagent, uniformly mixing, adding 4mL of 7.5% sodium carbonate solution after 7min, and measuring the light absorption value at 765nm after 1 h; the experimental results are as follows: through experimental detection, the total polyphenol content of the two solutions to be detected is from small to large as the bidirectional fermentation broth of the lucid ganoderma and the polygonatum sibiricum > culture medium which is not subjected to bidirectional fermentation, and the total polyphenol content of the fermentation broth subjected to the bidirectional fermentation of the lucid ganoderma and the polygonatum sibiricum is higher by comparing the fermentation broth subjected to the bidirectional fermentation of the lucid ganoderma and the culture medium which is not subjected to the bidirectional fermentation.
S4: detecting anti-inflammatory efficacy, namely detecting the hyaluronidase inhibition capability of bidirectional fermentation broth of ganoderma lucidum and polygonatum sibiricum, and preparing a reagent: hyaluronidase (I): the concentration is 500U/mL, the solution can be used as it is and can not stay overnight, and acetic acid buffer solution is used as solvent; sodium hyaluronate: 0.5mg/mL, prepared once and used for many times, and acetic acid buffer is used as a solvent; buffer: 4.8mL of solution A (0.2mol/L acetic acid 11.55mL glacial acetic acid dissolved in 1L distilled water); 45.2mL of solution B (0.2mol/L of sodium acetate 16.4g of anhydrous sodium acetate or 27.2g of sodium acetate trihydrate dissolved in 1L of distilled water) is mixed and diluted to 100mL to prepare an acetic acid buffer solution with the pH value of 5.6; acetylacetone solution: 50mL of 1.0mol/L sodium carbonate solution and 3.5mL of acetylacetone solution are mixed uniformly (prepared immediately before use); P-DAB color developing agent: dissolving 0.8g of p-dimethylaminobenzaldehyde in 15mL of concentrated hydrochloric acid and 15mL of absolute ethyl alcohol, and uniformly mixing; calcium chloride solution CaCl 2: 2.5 mol/L; sodium hydroxide solution NaOH: 5mol/L, the inhibition rate of the ganoderma lucidum and polygonatum sibiricum bidirectional fermentation broth on hyaluronidase increases along with the increase of the concentration of the ganoderma lucidum and polygonatum sibiricum bidirectional fermentation broth, iridin has an obvious inhibition effect on hyaluronidase, the inhibition rate reaches up to 82.3 percent, and the ganoderma lucidum and polygonatum sibiricum bidirectional fermentation broth has a good in-vitro anti-inflammatory effect.
S5: detecting the molecular weight of polysaccharide, and treating a sample: removing protein by adopting a sevage method, taking 5mL of a sample, adding 1mL of a prepared sevage reagent, oscillating, centrifuging, taking out an upper layer solution, and repeating the operation until no white substance appears in the middle; and (3) separation and purification of polysaccharide: pre-treating the gel, loading into a chromatographic column with the specification of 50 × 1.6cm and the flow rate of a pump of 0.5mL/min, and pumping a mobile phase to balance the chromatographic column; after the column is balanced, carrying out sample loading operation, taking samples to be separated, respectively preparing the samples into polysaccharide solutions of 5mg/mL, wherein the sample loading volume is 4mL, ultrapure water is used as an elution solvent, the flow rate is 1mL/min, collecting one tube every 10min, and collecting eluent; and (3) detecting the purity of the fraction: detecting the purity of the sample by adopting a gel permeation chromatography-eighteen-angle laser scattering combined system (GPC-MALS system), and measuring the purified sample by a GPC-MALS method through a water system filter head with the diameter of 0.22 mu m; a transfusion system: waters e 2695 system; gel chromatography column: shodex SUGER KS-805/KS-803; a detector: the eighteen-angle laser light scattering instrument is used together with a differential detector; sample introduction volume: 0.1 mL; flow rate: 1 mL/min; column temperature: 60 ℃; refractive index detector temperature: 50 ℃; mobile phase: a mixed solution of 0.1mol/L sodium nitrate solution and 0.02% sodium azide solution (mobile phase is filtered by a 0.22 mu m water system filter membrane in low vacuum degree); the experimental result shows that the molecular weight of the polysaccharide without the bidirectional fermentation culture medium is 21 percent, and the molecular weight of the polysaccharide of the bidirectional fermentation broth of the ganoderma lucidum and the polygonatum sibiricum is 60 percent.
S6: evaluating the human body moisturizing effect, and detecting the effect of the ganoderma lucidum and polygonatum sibiricum bidirectional fermentation liquid in cosmetics; after a subject cleans the face, the subject sits statically for 20min in a constant-temperature constant-humidity laboratory environment, data of water content, water dispersion loss and glossiness of the skin background are tested, the subject uses the lucid ganoderma and polygonatum sibiricum fermentation liquor mask, the mask is uncovered after 15min and is easy to absorb by the mask liquid, the moisture content value, the moisture dispersion loss, the skin chromaticity and the glossiness of the skin are respectively detected in time, 0.5h, 2h, 3h and 4h after the mask is used for 15min, and the experimental result shows that the lucid ganoderma and polygonatum sibiricum mask has a remarkable effect on improving the moisture content of the skin, the moisture content of the skin in time when the mask is used is improved from 46 to 72, and the moisture content of the skin is still higher than the moisture content of the skin when the mask is not used for 4h, so that the lucid ganoderma and polygonatum sibiricum fermentation liquor mask has a certain; after the mask with the lucid ganoderma and polygonatum sibiricum fermentation liquor is used, the water dispersion loss of the skin is slightly reduced, and the rising trend is avoided, so that a certain moisturizing effect is achieved; the skin glossiness of the ganoderma lucidum and polygonatum sibiricum fermentation liquor mask is improved before and after use; the lucid ganoderma and polygonatum sibiricum fermentation liquor mask can improve the skin whiteness within 4 hours; the ganoderma lucidum and polygonatum sibiricum fermentation liquor mask has a certain effect on reducing skin erythema; the effect of the ganoderma lucidum and rhizoma polygonati fermentation liquor mask on removing yellow is not obvious.
Through comparison of multiple sets of data, the third embodiment in the above embodiment is the best embodiment.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.
Claims (10)
1. A ganoderma-rhizoma polygonati bidirectional fermentation composition is characterized in that: the method comprises the following raw materials: 200.13 to 0.23 percent of distilled water U, 0.4 to 0.5 percent of 10 percent NaOH, 20 to 30 percent of lucid ganoderma, polygonatum odoratum and sealwort fermentation liquor compound liquid, 0.2 to 1.2 percent of fragrant fresh ketone and 0.03 to 0.08 percent of xanthan gum.
2. The ganoderma-polygonatum bidirectional fermentation composition according to claim 1, wherein the ganoderma-polygonatum bidirectional fermentation composition comprises: the lucid ganoderma and polygonatum odoratum compound liquid is prepared by the following method: respectively weighing 1, 3-butanediol and glycerol at a mass ratio of 1:1, uniformly mixing, preparing into polyol, respectively weighing polyol and lucid ganoderma and polygonatum odoratum fermentation liquor at a mass ratio of 3:7, and uniformly mixing.
3. The ganoderma-polygonatum bidirectional fermentation composition according to claim 1, wherein the ganoderma-polygonatum bidirectional fermentation composition comprises: the polygonatum sibiricum fermentation liquor compound liquid is prepared by the following method: respectively weighing 1, 3-butanediol and glycerol in a mass ratio of 1:1, uniformly mixing, and preparing into polyol; respectively weighing polyalcohol and ganoderma lucidum and rhizoma polygonati fermentation liquor according to the mass ratio of 3:7, and uniformly mixing.
4. A bidirectional fermentation process of Ganoderma lucidum-rhizoma Polygonati comprises the following steps:
s1: a ganoderma lucidum and polygonatum sibiricum bidirectional fermentation optimal process is characterized in that a liquid culture medium suitable for ganoderma lucidum is used as a bidirectional fermentation culture medium, polygonatum sibiricum extracting solution is added for bidirectional fermentation, and the concentration, the bacterial liquid inoculation amount and the fermentation pH value of the added polygonatum sibiricum extracting solution are determined through a single-factor experiment;
s2: performing expanded culture on the ganoderma lucidum and polygonatum sibiricum by using a 5L fermentation tank;
s3: detecting antioxidant effect, namely measuring the capacity of removing DPPH free radicals of sealwort stock solution, lucid ganoderma-sealwort fermentation liquor, lucid ganoderma-sealwort bidirectional fermentation liquor and culture medium which is not subjected to bidirectional fermentation at different concentrations respectively, and detecting the content of total polyphenol in the lucid ganoderma-sealwort bidirectional fermentation liquor and the culture medium which is not subjected to bidirectional fermentation respectively;
s4: detecting anti-inflammatory efficacy, namely detecting the hyaluronidase inhibition capability of the bidirectional fermentation broth of the ganoderma lucidum and the polygonatum sibiricum;
s5: detecting the molecular weight of the polysaccharide;
s6: evaluating the human body moisturizing effect, and detecting the effect of the ganoderma lucidum and polygonatum sibiricum bidirectional fermentation liquid in cosmetics.
5. The ganoderma-polygonatum bidirectional fermentation process according to claim 4, wherein the fermentation process comprises the following steps: the inoculation percentage content of the bacterial liquid in the S1 is 2% -10%, the pH value in the S1 is 5.2-9.2, and the concentration of the additive liquid of the yellow sperm in the S1 is 20% -100%.
6. The ganoderma-polygonatum bidirectional fermentation process according to claim 4, wherein the fermentation process comprises the following steps: the culture medium without bidirectional fermentation in S2: 5.0g of peptone, 10.0g of glucose, 5.0g of sodium chloride, 0.2g of calcium carbonate, 1.0L of distilled water, and pH7.2-7.4, wherein in S2, the bidirectional fermentation culture medium of ganoderma lucidum and polygonatum sibiricum: peptone 12.5g, glucose 25.0g, sodium chloride 12.5g, calcium carbonate 0.5g, distilled water 2.5L, rhizoma Polygonati powder: 50g, pH7.2-7.4, putting the prepared culture medium into a refrigerator at 4 ℃, and culturing in a ganoderma lucidum and polygonatum sibiricum fermentation tank in the same time as the bidirectional fermentation: inoculating the cultured secondary seed liquid into a 5L fermentation tank containing 2.5L culture medium in an inoculation amount of 10% (V/V), culturing at 26 deg.C and dissolved oxygen amount of 50-100% at 200r/min for 141h, and fermenting with Ganoderma and rhizoma Polygonati bidirectional fermentation liquid: culturing in fermentation tank, sterilizing at 120 deg.C under high temperature and high pressure for 30min, cooling, filtering with 0.2 μm filter plate, and adding 0.8% PEHG into the obtained fermentation broth for storage.
7. The ganoderma-polygonatum bidirectional fermentation process according to claim 4, wherein the fermentation process comprises the following steps: in the step S3, the ganoderma lucidum and polygonatum sibiricum fermentation liquor subjected to bidirectional submerged fermentation is diluted to the concentration of 1% -25%, and the culture medium which is not subjected to bidirectional fermentation is diluted to the concentration of 25% -100%, 1mL of a sample to be detected is taken, 5mL of 10% forskolin phenol reagent is respectively added into the sample to be detected, the mixture is uniformly mixed, 4mL of 7.5% sodium carbonate solution is added after 7min, and after 1h, the light absorption value is measured at 765 nm.
8. The ganoderma-polygonatum bidirectional fermentation process according to claim 4, wherein the fermentation process comprises the following steps: the concentration of the bidirectional fermentation liquor of the ganoderma lucidum and the polygonatum sibiricum in the S4 is 10-100%.
9. The ganoderma-polygonatum bidirectional fermentation process according to claim 4, wherein the fermentation process comprises the following steps: the molecular weight of the polysaccharide in the S5 culture medium without bidirectional fermentation is 21%, and the molecular weight of the polysaccharide in the ganoderma lucidum and polygonatum sibiricum bidirectional fermentation liquor is 60%.
10. The ganoderma-polygonatum bidirectional fermentation process according to claim 4, wherein the fermentation process comprises the following steps: s6, after cleaning the face, the subject sits statically for 20min in a constant-temperature and constant-humidity laboratory environment, and then data of water content, water dispersion loss and glossiness of the skin background are tested, the subject uses the ganoderma lucidum and polygonatum sibiricum fermentation liquor mask, the mask is removed after 15min, the skin is easy to absorb by the mask liquor, and the water content value, the water dispersion loss, the skin chromaticity and the glossiness of the skin are respectively tested when the mask is used for 0-4h after 15 min.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113230287A (en) * | 2021-06-01 | 2021-08-10 | 北京工商大学 | Application of Ganoderma and/or Inonotus obliquus in preparing anti-inflammatory factor product and regulating antibacterial peptide |
| CN113350251A (en) * | 2021-06-17 | 2021-09-07 | 山东福瑞达生物股份有限公司 | Fermented microemulsion with whitening effect and preparation method and application thereof |
| CN113750018A (en) * | 2021-04-22 | 2021-12-07 | 北京东方淼森生物科技有限公司 | Plant composition and preparation method and application thereof |
| CN118562914A (en) * | 2024-05-17 | 2024-08-30 | 中科药创(青岛)发酵工程有限公司 | A Ganoderma fermentation product containing selenium-enriched Ganoderma triterpene and polysaccharide for repairing liver injury, and its fermentation method |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103320474A (en) * | 2013-05-22 | 2013-09-25 | 甘肃农业大学 | Traditional Chinese medicinal bidirectional fermentation technology |
| CN103565944A (en) * | 2013-11-20 | 2014-02-12 | 青岛农业大学 | Bi-directional fermentation technology for traditional Chinese medicine |
| CN105193905A (en) * | 2014-06-27 | 2015-12-30 | 甘肃农业大学 | Traditional Chinese medicine processing technology of ganoderma-astragalus membranaceus bidirectional fermentation |
| CN105523794A (en) * | 2016-03-04 | 2016-04-27 | 王永产 | Ganoderma lucidum fungus culture medium and preparation method thereof |
| CN108066189A (en) * | 2017-12-19 | 2018-05-25 | 北京明弘科贸有限责任公司 | There is the anti-ageing ganoderma lucidum skin care compositions for dispelling yellow and its application |
| CN109528568A (en) * | 2018-12-18 | 2019-03-29 | 北京工商大学 | A kind of composition and preparation method thereof with anti-aging and white-skinned face function |
-
2019
- 2019-10-25 CN CN201911024210.3A patent/CN110755344A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103320474A (en) * | 2013-05-22 | 2013-09-25 | 甘肃农业大学 | Traditional Chinese medicinal bidirectional fermentation technology |
| CN103565944A (en) * | 2013-11-20 | 2014-02-12 | 青岛农业大学 | Bi-directional fermentation technology for traditional Chinese medicine |
| CN105193905A (en) * | 2014-06-27 | 2015-12-30 | 甘肃农业大学 | Traditional Chinese medicine processing technology of ganoderma-astragalus membranaceus bidirectional fermentation |
| CN105523794A (en) * | 2016-03-04 | 2016-04-27 | 王永产 | Ganoderma lucidum fungus culture medium and preparation method thereof |
| CN108066189A (en) * | 2017-12-19 | 2018-05-25 | 北京明弘科贸有限责任公司 | There is the anti-ageing ganoderma lucidum skin care compositions for dispelling yellow and its application |
| CN109528568A (en) * | 2018-12-18 | 2019-03-29 | 北京工商大学 | A kind of composition and preparation method thereof with anti-aging and white-skinned face function |
Non-Patent Citations (3)
| Title |
|---|
| 杨婧娟 等: "发酵对黄精主要活性成分及其抗氧化活性和刺激性的影响", 《食品工业科技》 * |
| 杨玉红 等: "《生命科学综合实验指导》", 31 July 2016, 东北大学出版社 * |
| 王建新: "《化妆品植物原料大全》", 30 June 2012, 中国纺织出版社 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113750018A (en) * | 2021-04-22 | 2021-12-07 | 北京东方淼森生物科技有限公司 | Plant composition and preparation method and application thereof |
| CN113750018B (en) * | 2021-04-22 | 2022-10-11 | 北京东方淼森生物科技有限公司 | Plant composition and preparation method and application thereof |
| CN113230287A (en) * | 2021-06-01 | 2021-08-10 | 北京工商大学 | Application of Ganoderma and/or Inonotus obliquus in preparing anti-inflammatory factor product and regulating antibacterial peptide |
| CN113350251A (en) * | 2021-06-17 | 2021-09-07 | 山东福瑞达生物股份有限公司 | Fermented microemulsion with whitening effect and preparation method and application thereof |
| CN118562914A (en) * | 2024-05-17 | 2024-08-30 | 中科药创(青岛)发酵工程有限公司 | A Ganoderma fermentation product containing selenium-enriched Ganoderma triterpene and polysaccharide for repairing liver injury, and its fermentation method |
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