CN109528568A - A kind of composition and preparation method thereof with anti-aging and white-skinned face function - Google Patents
A kind of composition and preparation method thereof with anti-aging and white-skinned face function Download PDFInfo
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- CN109528568A CN109528568A CN201811547331.1A CN201811547331A CN109528568A CN 109528568 A CN109528568 A CN 109528568A CN 201811547331 A CN201811547331 A CN 201811547331A CN 109528568 A CN109528568 A CN 109528568A
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9728—Fungi, e.g. yeasts
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
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- A61K8/9794—Liliopsida [monocotyledons]
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
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- A61Q19/08—Anti-ageing preparations
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/74—Biological properties of particular ingredients
- A61K2800/78—Enzyme modulators, e.g. Enzyme agonists
- A61K2800/782—Enzyme inhibitors; Enzyme antagonists
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/85—Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine
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Abstract
The present invention provides a kind of with the composition to delay senescence with white-skinned face function, the composition is obtained by ganoderma lucidum and radix polygonati officinalis powder by bi-directional fermentation, the addition of radix polygonati officinalis powder can significantly improve the yield of Ganoderma lucidum mycelium, its composition is removing DPPH, inhibit tyrosinase, there is significant effect in terms of improving Type I collagen content and polyoses content, and the harmful substance in Chinese medicine is effectively reduced by the product after bi-directional fermentation, it is set to effectively improve in terms of improving cell survival rate, absolutely prove that ganoderma lucidum-radix polygonati officinalis compound powder that the present invention is obtained by bi-directional fermentation has significant curative effect in terms of delaying senescence with whitening, it can be used as the functional materials for preparing related cosmetic material.
Description
Technical field
The invention belongs to bioengineering fields, are related to composition and its preparation side of a kind of anti-aging and white-skinned face function
Method, and in particular to a kind of that there is anti-aging and whitening function using Chinese medicine as what the microorganism bi-directional fermentation technology of raw material was prepared
Composition of effect and preparation method thereof.
Background technique
Despeckle beauty treatment, skin whitening, to delay senescence, remain youthful forever be thing that people dream of, people always thinking it is each
Method is planted to solve this problem.With advancing age, the ability of the natural free radical resisting of human body constantly declines, and cell constantly declines
Always, further prominent the problems such as pachylosis, pigmentation.China vast territory and abundant resources, possess miscellaneous natural resources, especially in
The traditional Chinese medicine of state it is anti-oxidant, in terms of have advantage outstanding, be widely used in delay senescence and skin whitening
Equal fields.Though Chinese medicine has advantageous advantage in this respect, how therefrom effective component to be extracted, it is maximum
Its effect of the performance of change is the target that current numerous scholars constantly study and pursue.Most commonly use the side of solvent extraction
Formula extracts active principle, separates and purifies, but its effect is limited, the low efficiency of extraction, and extraction process is complicated, at
This is very high.
Bi-directional fermentation technology refers to that with fungi be fermenting microbe, collectively forms hair using medicinal material or the dregs of a decoction as pharmacological property matrix
Ferment combines the mode fermented.Mycoplasma mainly has the effect of several aspects compared to crude drug after bi-directional fermentation: enhancing drug effect,
Expand purposes, mitigates toxicity." bi-directional fermentation engineering " illustrates combination of the medicinal fungi (containing other fungies) with Chinese medicine,
The problem that Wild Medicinal utilizes is overcome well, if the combination of this compatibility is correct, production method is reasonable, can also generate
A variety of effects such as improve effective component of chinese medicine content, generate new ingredient or drug action, increase curative effect or generate and coordinate to make
With, reduce the toxicity etc. of effective component of chinese medicine.
Patent application CN105213461A discloses a kind of utilization ganoderma lucidum-Radix Codonopsis bi-directional fermentation technology, is existed using Ganoderma Lucidum
A variety of ectoenzymes such as energy eccrine fiber element enzyme, peroxidase, superoxide dismutase, esterase, make it act on party in fermentation
Ginseng increases the variation of secondary metabolite to improve its drug eduction rate, and finally obtained product is at the anti-oxidant aspect the effect of
It significantly increases.Patent application CN105193905A discloses a kind of processing technique of Chinese herbs of ganoderma lucidum-Radix Astragali bi-directional fermentation simultaneously, yellow
It improves 20% or more after the antioxidant activity of stilbene is fermented and is easy to absorb；Patent application CN103316058 is also disclosed
A kind of ganoderma lucidum-Radix Angelicae Sinensis bi-directional fermentation technology improves 60-100 to improve its drug effect after the antioxidant activity of Radix Angelicae Sinensis is fermented
Times.The above-mentioned prior art has fully demonstrated bi-directional fermentation technology in enhancing drug effect, expands purposes, has in terms of mitigating toxicity significant
Effect, on the one hand the but above-mentioned prior art only focuses on bi-directional fermentation technology to the active raising of Chinese medicine, has ignored fungi
The application passes through bi-directional fermentation technology bring beneficial effect；Another aspect fungi and Chinese medicine carry out bi-directional fermentation and are different from fungi
Independent fermentation, wherein formation of the Chinese medicine form added for fungal mycelium, the effect of tunning has significant shadow
It rings, the above-mentioned prior art is not found the above problem to bi-directional fermentation process；Most of all, fungi and Chinese medicine
Specific choice has a significant impact for the effect of product.
Nature fungi is there are about 400,000 kinds, and wherein thousands of kinds of higher fungus, existing hyoscine reach hundreds of with edible fungi
Kind, it is known that nearly 400 kinds of the enzyme of fungi, the enzyme system of variety classes fungi is different, and Chinese medicine is at present up to 10,000 kinds of left sides
Right different types of ingredient is also different.If both sides' combined crosswise may constitute a large amount of different fementative compositions, can produce several
It is that an unbounded quantity of, ingredient, property are different " mycoplasma ", it is special that the mycoplasma that the combination of these different fermentations generates respectively has certain variation
How color is screened according to set clinical treatment target with indexs such as corresponding immune, drug effects, and obtain have effects that it is certain
Fermented product, then for further study to selected kind, exploitation is current cosmetic field research at novel fungitype product
Hot spot.
Ganoderma lucidum (Ganoderma lucidum) is referred to as the precious Chinese herbal medicine of " strengthening the body resistance to consolidate the constitution, strengthening by means of tonics ", genus polyporus bacterium
Section's medicinal fungi has been used as the diseases such as treatment hepatopathy, high cholesterol, hyperlipidemia.Main antioxidant active constituent in ganoderma lucidum
It is considered as ganodenic acid and ganoderma lucidum polysaccharide.Ganoderma lucidum polysaccharide can also remarkably promote cell nuclear dna synthesis function, and can promote thin
The division of born of the same parents to play the role of anti-aging, and has strengthen immunity.
Radix polygonati officinalis (Polygonatum odoratum (Mill.) Druce) is Liliaceae Polygonatum herbaceos perennial.Not
Name bell chain dish, tail ginseng, ground tube, Herba Hedyotis cantonensis root, Lian Zhu, Yu Can, seven, bamboo, ring Huang, hundred antidotes, luxuriant, solomon's seal etc..Because of plant
Glabrous, lotus is like small bamboo bar, the Ye Guangbao such as leaf of bamboo, and subterraneous root lotus is long and more piece, therefore named radix polygonati officinalis.First recorded in " legendary god of farming's book on Chinese herbal medicine
Through ", source area and domestication ground are China, and rhizome is one of Chinese medicine common drug, and medicinal history is long.It is advised in plain text as China
Fixed medicine and food raw materials have cultivation in most of areas of China.But some researches show that the polysaccharide of different sources radix polygonati officinalis contains
There are significant difference, quality of medicinal material is extremely unstable for amount and antioxidation activity in vitro.Its rhizome sweet in flavor and neutral in nature enters lung, stomach meridian, more
Rouge, it is soft and moist edible.Energy nourishing yin to moisten dryness, relieving restlessness are quenched the thirst.With help the five internal organs, nourishes qi and blood and dispel wind-heat and other effects.It can treat
Consumption of YIN caused by febrile disease, cough polydipsia, deficient fever, the paddy that disappears easily famine, frequent urination etc..Its adaptability of radix polygonati officinalis is wider, domestic in China
Most area can normal growth.According to investigations, China's pharmaceuticals industry exports about 3000-4000 tons of demand of radix polygonati officinalis
Ten thousand tons of about 300-500 of amount.With deep and international trade the development recognized radix polygonati officinalis effect, radix polygonati officinalis market conditions present vigorous
Situation, development and application prospect are concerned.
Summary of the invention
Based on the defect of the above-mentioned prior art, the present invention chooses ganoderma lucidum from numerous fungies and Chinese medicine and radix polygonati officinalis progress is two-way
Fermentation, wherein radix polygonati officinalis uses culture sill one of of the radix polygonati officinalis powder as bi-directional fermentation, can significantly improve ganoderma lucidum mycelium
Yield improves the oxidation resistance of glossy ganoderma fermentation product and inhibits the ability of tyrosinase, delays senescence to preferably realize
And the effect of whitening.
To achieve the goals above, the present invention provides a kind of with the composition to delay senescence with white-skinned face function, the combination
Object is prepared by ganoderma lucidum and radix polygonati officinalis powder by bi-directional fermentation technology.
Further, the radix polygonati officinalis powder is crossed 90-120 mesh and is obtained by radix polygonati officinalis grinds.
Further, the radix polygonati officinalis place of production is Hunan.
Further, the composition is prepared by following step:
(1) preparation of culture medium: peptone, glucose, sodium chloride, calcium carbonate, radix polygonati officinalis powder are dissolved in distilled water, are adjusted
pH；
(2) fermentation of ganoderma lucidum: ganoderma lucidum is inoculated in the culture medium of step (1) preparation and carries out fermented and cultured；
(3) it collects fermentation liquid: by the fermentation liquid plate-frame filtering of step (2), collecting fermentation liquid to obtain the final product.
Further, the specific preparation method of culture medium in the step (1) are as follows: in parts by weight, peptone 12-
18 parts, 27-33 parts of glucose, 12-18 parts of sodium chloride, 0.55-0.65 parts of calcium carbonate, 55-65 parts of radix polygonati officinalis powder, are dissolved in distilled water
2800-3200 parts, adjust pH to 7.2-7.4.
Further, ganoderma lucidum is being inoculated in culture medium before fermentation, is first being accessed Ganoderma Lucidum solid in the step (2)
Passage activation 3-5 generation in body slant medium.
Further, the activation passage temperature is 25-28 DEG C, and the time is 8-12 days.
Further, in the step (2) ganoderma lucidum inoculation quality be culture volume 5-8%.
Further, in the step (2) ganoderma lucidum fermentation condition specifically: ganoderma lucidum is inoculated in culture medium, in 22-
28 DEG C of culture 130-145h.
Further, the dissolved oxygen amount of fermentation process is 45-55%, revolving speed 180-250rpm in the step (2).
The present invention also provides the preparation methods of above-mentioned composition, specifically includes the following steps:
(1) preparation of culture medium: peptone, glucose, sodium chloride, calcium carbonate, radix polygonati officinalis powder are dissolved in distilled water, are adjusted
pH；
(2) fermentation of ganoderma lucidum: ganoderma lucidum is inoculated in the culture medium of step (1) preparation and carries out fermented and cultured；
(3) it collects fermentation liquid: by the fermentation liquid plate-frame filtering of step (2), collecting fermentation liquid to obtain the final product.
Further, the preparation method of the composition, comprising the following steps:
(1) preparation of culture medium: in parts by weight, 12-18 parts of peptone, 27-33 parts of glucose, sodium chloride 12-
18 parts, 0.55-0.65 parts of calcium carbonate, 55-65 parts of radix polygonati officinalis powder are dissolved in 2800-3200 parts of distilled water, adjust pH to 7.2-7.4；
(2) fermentation of ganoderma lucidum: ganoderma lucidum is inoculated in the culture medium of step (1) preparation, and ganoderma lucidum inoculation quality is culture medium
5-8%, in 22-28 DEG C of culture 130-145h, wherein dissolved oxygen amount is 45-55%, revolving speed 180-250rpm；
(3) it collects fermentation liquid: by the fermentation liquid plate-frame filtering of step (2), collecting fermentation liquid to obtain the final product.
Have function of delaying senility in preparation the present invention also provides above-mentioned composition and/or has the cosmetics of white-skinned face function
In application.
The invention has the benefit that
(1) by bi-directional fermentation technology, ganoderma lucidum and radix polygonati officinalis are fermented, discovery ganoderma lucidum obtains with radix polygonati officinalis powder bi-directional fermentation
Ability of the composition in terms of removing DPPH, inhibiting tyrosinase be substantially better than ganoderma lucidum and individually ferment, and bi-directional fermentation
The content of collagen is higher in fermentation liquid.The cell survival rate when diluted concentration is 1%, 0.5%, 0.025% adds radix polygonati officinalis
The un-added cell survival rate of the glossy ganoderma fermentation liquor ratio of powder is high, illustrates that the method for bi-directional fermentation reduces the poison of effective component of chinese medicine
Property.Ganoderma lucidum radix polygonati officinalis powder co-fermentation liquid greatly improving compared with the total starches that ganoderma lucidum is individually fermented, can be common by ganoderma lucidum radix polygonati officinalis powder
Fermentation higher efficiency obtains ganoderma lucidum polysaccharide product.Ganoderma lucidum and the product that radix polygonati officinalis powder is obtained by bi-directional fermentation are absolutely proved above
It has potential application, is can be widely applied in the preparation of cosmetics in terms of delaying senescence with whitening.
(2) present invention studies the addition manner of radix polygonati officinalis in bi-directional fermentation, and discovery is extracted compared to by radix polygonati officinalis
Object makes an addition in culture medium, and the production of Ganoderma lucidum mycelium can be significantly improved by directly making an addition in culture medium ferment using radix polygonati officinalis powder
Rate, with better effect in terms of removing DPPH, inhibiting tyrosinase, reduce.
Detailed description of the invention
Fig. 1 is that experimental example 5 is fitted obtained Type I collagen standard curve.
Specific embodiment
Embodiment 1 has anti-aging, the composition of white-skinned face function and its preparation
Composition: ganoderma lucidum, radix polygonati officinalis powder are prepared by bi-directional fermentation technique, and wherein the place of production of radix polygonati officinalis is Hubei, specifically
It is as follows:
(1) preparation of culture medium: in parts by weight, 15 parts of peptone, 30 parts of glucose, 15 parts of sodium chloride, carbonic acid
0.6 part of calcium, 60 parts of radix polygonati officinalis powder are dissolved in 3000 parts of distilled water, adjust pH to 7.2；
(2) fermentation of ganoderma lucidum: ganoderma lucidum 4 generation of secondary culture in slant medium, cultivation temperature are 28 DEG C, and incubation time is
It 10 days, is then inoculated in the fluid nutrient medium of step (1) preparation, ganoderma lucidum inoculation quality is the 6% of culture medium, is cultivated in 25 DEG C
138h, wherein dissolved oxygen amount is 50%, revolving speed 200rpm；
(3) it collects fermentation liquid: by the fermentation liquid plate-frame filtering of step (2), collecting fermentation liquid to obtain the final product.
Embodiment 2 has anti-aging, the composition of white-skinned face function and its preparation
Composition: ganoderma lucidum, radix polygonati officinalis powder are prepared by bi-directional fermentation technique, and wherein the place of production of radix polygonati officinalis is Hubei, specifically
It is as follows:
(1) preparation of culture medium: in parts by weight, 12 parts of peptone, 27 parts of glucose, 12 parts of sodium chloride, carbonic acid
0.55 part of calcium, 55 parts of radix polygonati officinalis powder are dissolved in 2800 parts of distilled water, adjust pH to 7.2；
(2) fermentation of ganoderma lucidum: ganoderma lucidum 3 generation of secondary culture in slant medium, cultivation temperature are 25 DEG C, and incubation time is
It 12 days, is then inoculated in the fluid nutrient medium of step (1) preparation, ganoderma lucidum inoculation quality is the 5% of culture medium, is cultivated in 22 DEG C
130h, wherein dissolved oxygen amount is 45%, revolving speed 180rpm；
(3) it collects fermentation liquid: by the fermentation liquid plate-frame filtering of step (2), collecting fermentation liquid to obtain the final product.
Embodiment 3 has anti-aging, the composition of white-skinned face function and its preparation
Composition: ganoderma lucidum, radix polygonati officinalis powder are prepared by bi-directional fermentation technique, and wherein the place of production of radix polygonati officinalis is Hubei, specifically
It is as follows:
(1) preparation of culture medium: in parts by weight, 18 parts of peptone, 33 parts of glucose, 18 parts of sodium chloride, carbonic acid
0.65 part of calcium, 65 parts of radix polygonati officinalis powder are dissolved in 3200 parts of distilled water, adjust pH to 7.4；
(2) fermentation of ganoderma lucidum: ganoderma lucidum 5 generation of secondary culture in slant medium, cultivation temperature are 26 DEG C, and incubation time is
It 8 days, is then inoculated in the fluid nutrient medium of step (1) preparation, ganoderma lucidum inoculation quality is the 8% of culture medium, is cultivated in 28 DEG C
145h, wherein dissolved oxygen amount is 55%, revolving speed 250rpm；
(3) it collects fermentation liquid: by the fermentation liquid plate-frame filtering of step (2), collecting fermentation liquid to obtain the final product.
1 ganoderma lucidum of comparative example individually ferment formation composition and its preparation
Composition is individually fermented using ganoderma lucidum, and composition is prepared, and wherein the place of production of radix polygonati officinalis is Hubei, specific as follows
(1) culture medium is prepared: 15 parts of peptone, 30 parts of glucose, 15 parts of sodium chloride, 0.6 part of calcium carbonate, distilled water 3000
Part, adjust pH to 7.2.
(2) fermentation of ganoderma lucidum: ganoderma lucidum is inoculated in the culture medium of step (1) preparation, and ganoderma lucidum inoculation quality is culture medium
6%, in 25 DEG C of culture 164h, wherein dissolved oxygen amount is 50%, revolving speed 200rpm；
(3) it collects fermentation liquid: by the fermentation liquid plate-frame filtering of step (2), collecting fermentation liquid to obtain the final product.
Comparative example 2 carries out the composition and its preparation that bi-directional fermentation obtains using radix polygonati officinalis extracting solution
Composition: it is prepared into the product that culture medium cultivates ganoderma lucidum by adding radix polygonati officinalis extracting solution, wherein beautiful
The place of production of bamboo is Hubei, and specific preparation process is as follows:
(1) preparation of radix polygonati officinalis extracting solution: the place of production is that the radix polygonati officinalis in Hunan is clayed into power, and be sieved 100 mesh.Fragrant Solomonseal Rhizome and distillation
The solid-liquid ratio of water is 1:60 in 80 DEG C of extraction 2.5h.
(2) ganoderma lucidum-radix polygonati officinalis extracts liquid culture medium and prepares: 12 parts of peptone, 24 parts of glucose, 12 parts of sodium chloride, calcium carbonate
0.48 part, 2400 parts of radix polygonati officinalis extracting solution adjust pH7.2.
(3) fermentation of ganoderma lucidum: ganoderma lucidum is inoculated in the culture medium of step (1) preparation, and ganoderma lucidum inoculation quality is culture medium
6%, in 25 DEG C of culture 164h, wherein dissolved oxygen amount is 50%, revolving speed 200rpm；
(4) it collects fermentation liquid: by the fermentation liquid plate-frame filtering of step (3), collecting fermentation liquid to obtain the final product.
Comparative example 3 replaces ganoderma lucidum to carry out the composition and its preparation that bi-directional fermentation is prepared using Poria cocos
In addition to replacing ganoderma lucidum with Poria cocos, remaining is with embodiment 1, and wherein the place of production of radix polygonati officinalis is Hubei, specific preparation process are as follows:
(1) preparation of culture medium: in parts by weight, 15 parts of peptone, 30 parts of glucose, 15 parts of sodium chloride, carbonic acid
0.6 part of calcium, 60 parts of radix polygonati officinalis powder are dissolved in 3000 parts of distilled water, adjust pH to 7.2；
(2) fermentation of Poria cocos: Poria cocos is inoculated in the culture medium of step (1) preparation, and Poria cocos inoculation quality is culture medium
6%, in 25 DEG C of culture 138h, wherein dissolved oxygen amount is 50%, revolving speed 200rpm；
(3) it collects fermentation liquid: by the fermentation liquid plate-frame filtering of step (2), collecting fermentation liquid to obtain the final product.
Comparative example 4 replaces radix polygonati officinalis to carry out the composition and its preparation that bi-directional fermentation is prepared using Radix Codonopsis
In addition to replacing radix polygonati officinalis with Radix Codonopsis, remaining is with embodiment 1, and wherein the place of production of radix polygonati officinalis is Hubei, specific preparation process are as follows:
(1) preparation of culture medium: in parts by weight, 15 parts of peptone, 30 parts of glucose, 15 parts of sodium chloride, carbonic acid
0.6 part of calcium, 60 parts of codonopsis pilosula powder are dissolved in 3000 parts of distilled water, adjust pH to 7.2；
(2) fermentation of ganoderma lucidum: ganoderma lucidum is inoculated in the culture medium of step (1) preparation, and ganoderma lucidum inoculation quality is culture medium
6%, in 25 DEG C of culture 138h, wherein dissolved oxygen amount is 50%, revolving speed 200rpm；
(3) it collects fermentation liquid: by the fermentation liquid plate-frame filtering of step (2), collecting fermentation liquid to obtain the final product.
Comparative example 5 uses the place of production to carry out composition and its preparation that bi-directional fermentation is prepared for the radix polygonati officinalis in Hubei
In addition to the radix polygonati officinalis for the place of production being Hubei, remaining is the same as embodiment 1, specific preparation process are as follows:
(1) preparation of culture medium: in parts by weight, 15 parts of peptone, 30 parts of glucose, 15 parts of sodium chloride, carbonic acid
0.6 part of calcium, 60 parts of codonopsis pilosula powder are dissolved in 3000 parts of distilled water, adjust pH to 7.2；
(2) fermentation of ganoderma lucidum: ganoderma lucidum is inoculated in the culture medium of step (1) preparation, and ganoderma lucidum inoculation quality is culture medium
6%, in 25 DEG C of culture 138h, wherein dissolved oxygen amount is 50%, revolving speed 200rpm；
(3) it collects fermentation liquid: by the fermentation liquid plate-frame filtering of step (2), collecting fermentation liquid to obtain the final product.
Each fermentation process mycelia yield of test example 1
Each fermentation process mycelia yield of table 1
Table 1 statistics indicate that, using radix polygonati officinalis powder carry out bi-directional fermentation, mycelium production be apparently higher than ganoderma lucidum individually ferment, jade
Bamboo extracting solution bi-directional fermentation.
2 DPPH clearance rate of test example measures and compares
2.1 preparation of reagents
(1) the DPPH ethanol solution of 0.2mM/L is prepared: weigh 20mgDPPH, dehydrated alcohol dissolution is added and constant volume in
In 250mL volumetric flask, and 4 DEG C are kept in dark place.
(2)100μg/Ml VCSolution is prepared: weighing 25mgVC, distilled water is added and dissolves and is dissolved in 250mL volumetric flask surely,
It is kept in dark place.
(3) sample concentration: 0-10mg/mL (aqueous samples with water-soluble, alcohol-soluble sample alcohol is molten)
2.2 experimentation
(1) the DPPH solution of the prepare liquid and 1.0mL that take 1.0mL mixes (A pipe)；
(2) the DPPH solution of the water and 1.0mL that take 1.0mL mixes (B pipe)；
(3) prepare liquid of the ethanol solution and 1.0mL that take 1.0mL mixes (C pipe)；
(4) after dark place reaction 30min, A, B, C pipe absorbance value are surveyed at 517nm；
(5) clearance rate: clearance rate (%)=[(B+C)-A]/B*100% is calculated
It is loaded table: (aqueous sample)
2.3 experimental result
The DPPH clearance rate of fermentation liquid is prepared in 2 different fermentations technique of table
Table 2 statistics indicate that, the even ganoderma lucidum radix polygonati officinalis fermentation liquid of low concentration, DPPH clearance rate is also relatively high, mentions
Show that ganoderma lucidum radix polygonati officinalis bi-directional fermentation liquid has better DPPH elimination effect, oxidation resistant effect can be better played.
3 tyrosinase Inhibition test of test example
The configuration of 3.1 reagents
(1) PBS phosphate buffer configures:
1. take 17.91g disodium hydrogen phosphate to be dissolved in distilled water is dissolved in 500mL surely；
2. take 7.8g sodium dihydrogen phosphate dihydrate to be dissolved in distilled water is dissolved in 500mL surely；
The solution 92.6mL and 107.4mL that 1. 2. configure is taken to be made into 200mL, the PBS phosphate buffer of pH6.8 respectively.
(2) hydrochloric acid solution of 0.1mol/L:
The HCl solution 0.862mL for being 36%-38% with HCl content, distilled water are settled to 100mL.
(3) 0.05%L- tyrosine solution:
0.05g l-tyrosine is taken, the HCl solution of 35mL 0.1mol/L is first dissolved in, adds the PBS phosphorus of 65mL pH6.8
Acid buffer, total 100ml.
(4) 0.1% arbutin solution (positive control):
The PBS phosphate buffer that 0.015g arbutin is dissolved in 15ml pH6.8 is weighed, is mixed.
3.2 experimentation
Reagent is added according to following table to be tested:
Sample is calculated to the maximum inhibition T (%) of tyrosinase.
Formula: T (%)=[(A-B)-(C-D)]/(A-B) × 100%
3.3 experimental result
The tyrosinase activity inhibitory effect of fermentation liquid is prepared in 3 different fermentations technique of table
Table 3 statistics indicate that, radix polygonati officinalis provided by the invention-ganoderma lucidum bi-directional fermentation liquid with good tyrosinase activity inhibit
Effect, the effect that its whitening can effectively be promoted in cosmetics by making an addition to are not had completely if individually fermented using ganoderma lucidum
The function of inhibiting tyrosinase activity carries out bi-directional fermentation using radix polygonati officinalis extracting solution, and inhibitory activity is significantly reduced, and beautiful
The place of production of bamboo also has a significant impact for the activity of its final fermentation liquid.
The experiment of 4 fibroblast of test example
4.1 experimental procedure
(1) bed board
Cell is taken out, cell growth state is observed, outwells culture medium, cleaned with PBS, outwell PBS, digested with pancreatin.Add
DMEM terminates digestion, blows and beats bottle wall, and cell is moved to centrifuge tube, is centrifuged 5 minutes, 210rpm.Supernatant is poured out, culture medium is added to blow
Cell is beaten, keeps cell uniform.It takes 100 microlitres to add another small test tube, therefrom takes 10 microlitres.It gets in cell counting board.It calculates
Cell density.
Take 96 orifice plates.Every hole makes a call to 100 microlitres, and about 10000 cells observe the cell density in every hole, it is ensured that every hole is thin
Born of the same parents' quantity is similar, is incubated overnight.
(2) it is administered
Extracting solution is taken to cross in film to the EP pipe to have sterilized.EP pipe after separately taking 1 sterilizing, is added 900 μ L DMEM, and 100
μ L extracting solution, diluted concentration 8%.Then double of diluted concentration is respectively 4%, 2%, 1%, 0.5%, 0.25%.
Culture solution is sucked out, every hole adds 100 μ L sample reagents, and DMEM is blank control.
(3) add CCK-8
It after for 24 hours, is protected from light, every hole adds 10 μ L CCK-8, surveys light absorption value at 450 nm after 1 hour.
4.2 experimental result
The fibroblast survival rate of fermentation liquid is prepared in 4 different fermentations technique of table
Table 4 statistics indicate that, the composition that radix polygonati officinalis provided by the invention-ganoderma lucidum bi-directional fermentation obtains can be significantly at fiber finer
Born of the same parents' survival rate has facilitation to fibroblast proliferation, is conducive to the generation of activity of fighting against senium ingredient.
The experiment of 5 collagen of test example
5.1 experimental procedure
(1) fibroblast bed board and dosing
Fibroblast is with 10 × 104The density of/mL is laid on 6 orifice plates, and 2mL cell suspending liquid is added in every hole, pastes to cell
Dosing is used for after wall.Totally 3 groups of experimental groups: negative control, 1% ganoderma lucidum radix polygonati officinalis fermentation liquid, 1% ganoderma lucidum fermented liquid are set.
(2) COL-1 is tested
After drug effect for 24 hours after, take every group of cell supernatant, be centrifuged 15min in 5 DEG C of 1000rpm, its supernatant is taken after centrifugation
For testing.It is tested according to Type I collagen detection kit operating process.Firstly, first fit standard curve, on this basis
Calculate the content of sample Type I collagen.
5.2 experimental result
(1) curve matching
It is fitted using computer, as a result such as attached drawing 1.
(2) influence of the sample to fibroblasts to secrete Type I collagen
The influence to fibroblasts to secrete Type I collagen of fermentation liquid is prepared in 5 different fermentations technique of table
| Title | Concentration | Test Type I collagen content/ngmL-1 |
| Negative control | - | 329.2933 |
| Embodiment 1 | 1% (v/v) | 223.6418 |
| Embodiment 2 | 1% (v/v) | 207.4333 |
| Embodiment 3 | 1% (v/v) | 212.7650 |
| Comparative example 1 | 1% (v/v) | 53.0629 |
| Comparative example 2 | 1% (v/v) | 153.9037 |
| Comparative example 3 | 1% (v/v) | 57.4097 |
| Comparative example 4 | 1% (v/v) | 88.4442 |
| Comparative example 5 | 1% (v/v) | 168.4693 |
It is above-mentioned statistics indicate that, in the composition that radix polygonati officinalis provided by the invention-ganoderma lucidum bi-directional fermentation obtains contain a large amount of glue
Original can be effectively improved skin elasticity, have good activity of fighting against senium, if not by the way of bi-directional fermentation or adopted
The radix polygonati officinalis for replacing radix polygonati officinalis with other Chinese medicines, all significantly reducing the collagen content in fermentation liquid, and use in bi-directional fermentation produces
Ground and addition manner have great influence for tunning.
6 determination of polysaccharide of test example
6.1 experimental principle
Polysaccharide is first hydrolyzed into monosaccharide under effect of sulfuric acid, and rapid dehydration generates alditol derivative, generates with phenol reactant
Orange solution has characteristic absorption at 490nm, quantitative compared with standard curve.
The measurement of 6.2 glucose standard curves
Glucose m=50mg is weighed, the amount v=50mL of distilled water is added, sugared concentration is 1mg/mL (first wife's Glucose Liquid)
Solution sugar concentration is 0.2mg/mL after taking first wife's liquid v=2000uL distilled water v=8000uL to be diluted.
The solution prepared is put into boiling water bath and heats 30min, it is cooling in cold water after taking-up；It is measured under 490nm wavelength
Absorbance, using concentration as abscissa, absorbance is ordinate, draws standard curve, obtains standard curve y=9.2568x-
0.0282, R2=0.9993.
6.3 experimentation
Take sample appropriate (general 100uL), 1900uL water be added, mix, be added 5% phenol, concussion uniformly, along wall plus
Enter 5mL sulfuric acid, be uniformly mixed, be put into boiling water bath and react 30min, is put into cooling in cold water after reaction, is measured under 490nm
Absorbance.
6.4 experimental result
The polyoses content in fermentation liquid is prepared in 6 different fermentations technique of table
| Title | Polyoses content/mgL-1 |
| Embodiment 1 | 10.65 |
| Embodiment 2 | 9.76 |
| Embodiment 3 | 8.44 |
| Comparative example 1 | 0.99 |
| Comparative example 2 | 6.53 |
| Comparative example 3 | 0.54 |
| Comparative example 4 | 4.87 |
| Comparative example 5 | 4.54 |
It is above-mentioned statistics indicate that, containing a large amount of more in the composition that radix polygonati officinalis provided by the invention-ganoderma lucidum bi-directional fermentation obtains
Sugar, polysaccharide have the multiple efficacies such as anti-oxidant, can also be applied to as multiple-effect function factor in cosmetics, especially oxidation resistant
Activity.But if replacing radix polygonati officinalis not by the way of bi-directional fermentation or using other Chinese medicines, it can all significantly reduce fermentation
Polyoses content in liquid, and the radix polygonati officinalis place of production used in bi-directional fermentation and addition manner have important shadow for tunning
It rings.
Above-mentioned detailed description is illustrating for one of them possible embodiments of the present invention, the embodiment not to
The scope of the patents of the invention is limited, all equivalence enforcements or change without departing from carried out by the present invention are intended to be limited solely by the technology of the present invention
In the range of scheme.
Claims (10)
1. a kind of with the composition to delay senescence with white-skinned face function, which is characterized in that the composition is by ganoderma lucidum and radix polygonati officinalis powder
It is prepared by bi-directional fermentation technology.
2. the composition of anti-aging and white-skinned face function according to claim 1, which is characterized in that the radix polygonati officinalis powder is by jade
Bamboo grinds are crossed 90-120 mesh and are obtained.
3. the composition of anti-aging and white-skinned face function according to claim 2, which is characterized in that the radix polygonati officinalis place of production is
Hunan.
4. the composition of anti-aging and white-skinned face function according to claim 1, which is characterized in that the composition passes through
Following step is prepared:
(1) preparation of culture medium: peptone, glucose, sodium chloride, calcium carbonate, radix polygonati officinalis powder are dissolved in distilled water, adjust pH；
(2) fermentation of ganoderma lucidum: ganoderma lucidum is inoculated in the culture medium of step (1) preparation and carries out fermented and cultured；
(3) it collects fermentation liquid: by the fermentation liquid plate-frame filtering of step (2), collecting fermentation liquid to obtain the final product.
5. the composition of anti-aging and white-skinned face function according to claim 4, which is characterized in that in the step (1)
The specific preparation method of culture medium are as follows: in parts by weight, 12-18 parts of peptone, 27-33 parts of glucose, sodium chloride 12-18
Part, 0.55-0.65 parts of calcium carbonate, 55-65 parts of radix polygonati officinalis powder are dissolved in 2800-3200 parts of distilled water, adjust pH to 7.2-7.4.
6. the composition of anti-aging and white-skinned face function according to claim 4, which is characterized in that in the step (2)
The inoculation quality of ganoderma lucidum is the 5-8% of culture volume.
7. the composition of anti-aging and white-skinned face function according to claim 6, which is characterized in that in the step (2)
The fermentation condition of ganoderma lucidum specifically: ganoderma lucidum is inoculated in culture medium, in 22-28 DEG C of culture 130-145h.
8. the composition of anti-aging and white-skinned face function according to claim 7, which is characterized in that in the step (2)
The dissolved oxygen amount of fermentation process is 45-55%, revolving speed 180-250rpm.
9. a kind of preparation method of claim 1-8 described in any item anti-aging and the composition of white-skinned face function, feature
It is, comprising the following steps:
(1) preparation of culture medium: peptone, glucose, sodium chloride, calcium carbonate, radix polygonati officinalis powder are dissolved in distilled water, adjust pH；
(2) fermentation of ganoderma lucidum: ganoderma lucidum is inoculated in the culture medium of step (1) preparation and carries out fermented and cultured；
(3) it collects fermentation liquid: by the fermentation liquid plate-frame filtering of step (2), collecting fermentation liquid to obtain the final product.
10. any one of the claim 1-8 composition has function of delaying senility in preparation and/or has the change of white-skinned face function
Application in cosmetic.
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| CN113101256A (en) * | 2021-05-12 | 2021-07-13 | 山东福瑞达生物股份有限公司 | Anti-aging repair nanoemulsion and application thereof |
| CN113350251A (en) * | 2021-06-17 | 2021-09-07 | 山东福瑞达生物股份有限公司 | Fermented microemulsion with whitening effect and preparation method and application thereof |
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| CN113101256A (en) * | 2021-05-12 | 2021-07-13 | 山东福瑞达生物股份有限公司 | Anti-aging repair nanoemulsion and application thereof |
| CN113350251A (en) * | 2021-06-17 | 2021-09-07 | 山东福瑞达生物股份有限公司 | Fermented microemulsion with whitening effect and preparation method and application thereof |
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