CN104644538A - Scutellaria baicalensis and lactic acid bacteria yeasts, cosmetics containing yeasts, preparation method and application - Google Patents
Scutellaria baicalensis and lactic acid bacteria yeasts, cosmetics containing yeasts, preparation method and application Download PDFInfo
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Abstract
The invention belongs to the field of biological fermentation and in particular relates to scutellaria baicalensis and lactic acid bacteria yeasts, cosmetics containing yeasts, a preparation method and application. According to the technical problem to be solved, the biological transformation theory is taken as the guidance, a product with the effects of whitening, removing acnes and controlling oil is provided by adopting a liquid fermentation system of scutellaria baicalensis and lactic acid bacteria. The scutellaria baicalensis and lactic acid bacteria yeast refers to a fermented liquid mixture obtained by the step of fermenting a culture medium consisting of scutellaria baicalensis and water with lactic acid bacteria, wherein the culture medium comprises 5-10 weight percent of scutellaria baicalensis based on the weight of a crude drug, and the inoculation amount of the lactic acid bacteria is 1.0*10<4> to 1.0*10<8>cfu/ml.
Description
Technical field
The invention belongs to biological fermentation field, be specifically related to Radix Scutellariae lactobacillus fermented product, containing the cosmetics of fermented product and preparation method and purposes.
Background technology
" Chinese Pharmacopoeia " is recorded, and Radix Scutellariae nature and flavor are bitter, cold.Return lung, gallbladder, spleen, large intestine, small intestine meridian.Heat clearing and damp drying, eliminating fire and detoxication, hemostasis, antiabortive.Wet for hygropyrexia, administration, vomiting uncomfortable in chest, damp and hot feeling of fullness, dysentery, jaundice, cough due to lung-heat, high hot excessive thirst, heat in blood tells nosebleed, carbuncle sore tumefacting virus, frequent fetal movement.Compendium of Material Medica is recorded: " Radix Scutellariae is bitter, flat, nontoxic "." herbal classic " is recorded: " cure mainly all hot jaundices, intestinal lets out dysentery, relieves oedema or abdominal distension through diuresis or purgation, and hematochezia closes, malignant boil cellulitis erosion acute scleritis "." not Lu " be recorded: " the hot pathogenic heat in the stomach of main treatment expectorant, little angina abdominis, rapid digestion of food, sharp small intestinal, woman's blood closes, and drenches dew hematochezia, abdominal pain in children ".Show according to modern pharmacology research, Radix Scutellariae have antitumor, antibacterial, blood fat reducing, protect the liver, the effect such as function of gallbladder promoting, antioxidation.Radix Scutellariae, as Chinese medicine material, all has good curative effect to the treatment of various diseases clinically.In recent years, the application of Radix Scutellariae in cosmetics gets more and more, and its whitening function also has correlational study and application.
Lactobacillus is a kind of probiotic bacteria be present in mankind's body, the general name of one class without spore, gram-positive bacterium of to be fermenting carbohydrate primary product be lactic acid, at least can be divided into 18 genus at present, have kind more than 200, the overwhelming majority is all essential in human body and has the flora of important physiological function, is extensively present in the intestinal of human body.Lactobacillus to utilize the antibacterial producing lactic acid in the sweat of glucose or lactose.It is not only research classification, biochemistry, heredity, molecular biology and engineered ideal material, there is important learning value in theory, and also high with human lives's closely-related key areas using value at industry, farming and animal husbandry, food and medicine etc.At present, lactobacillus is applied all to some extent in whitening skin and preserving moisture cosmetics.
But Radix Scutellariae and lactobacillus are showed no the report of whitening and anti-acne control wet goods effect.
Summary of the invention
Technical problem solved by the invention is with " biological Transformation Theory " for instructing, and the liquid fermentation system of employing Radix Scutellariae and lactobacillus provides a kind of product with whitening, anti-acne control oil effect.
First technical problem solved by the invention is to provide the Radix Scutellariae lactobacillus fermented product of the liquid fermentation gained of Radix Scutellariae and lactobacillus.
Radix Scutellariae lactobacillus fermented product of the present invention, with Radix Scutellariae and water composition culture medium, the then liquid fermentation mixture of inoculating lactic acid bacterium fermentation gained; Wherein, culture medium is with crude drug weighing scale, and Radix Scutellariae percentage by weight is 3 ~ 8%; The inoculum concentration of lactobacillus is 1.0 × 10
4~ 1.0 × 10
8cfu/ml.
In technique scheme, culture medium is with crude drug weighing scale, and Radix Scutellariae percentage by weight is 5%.Radix Scutellariae is primary crude drug or baikal skullcap root decoction pieces.
In technique scheme, the inoculum concentration of lactobacillus is preferably 1.0 × 10
6cfu/ml.
Second technical problem solved by the invention is to provide the preparation method of Radix Scutellariae lactobacillus fermented product of the present invention, comprises the following steps:
A, with Radix Scutellariae and water composition culture medium, with crude drug weighing scale, Radix Scutellariae percentage by weight is 3 ~ 8%;
Inoculating lactic acid bacterium after B, sterilizing, inoculum concentration is 1.0 × 10
4~ 1.0 × 10
8cfu/ml;
C, cultivate under the condition of 30 DEG C ~ 40 DEG C and 0-200rpm after 24 ~ 72 hours and namely obtain Radix Scutellariae lactobacillus fermented product.
In technique scheme, culture medium is with crude drug weighing scale, and Radix Scutellariae percentage by weight is 5%.
In technique scheme, the inoculum concentration of lactobacillus is preferably 1.0 × 10
6cfu/ml.
In technique scheme, " after sterilizing inoculating lactic acid bacterium " described in step B refers at 105 DEG C-115 DEG C sterilizing 20-40min, inoculates lactobacillus after cooling.Further preferably, described " after sterilizing inoculating lactic acid bacterium " refers at 115 DEG C of sterilizing 30min, inoculates lactobacillus after cooling.
In technique scheme, step C cultivates 72 hours under being preferable over the condition of 37 DEG C.
In technique scheme, the lactic acid bacteria culturers that inoculation adopts can be cultivated the strain obtained, as the lactobacillus inoculation etc. for the manufacture of Pickles, Yoghourt by choice for use at present by various usual way.
Wherein, a kind of preferred strain source and training method are:
A, in solid medium, cultivate lactic acid bacteria culturers;
B, by steps A cultivate lactobacillus inoculum enter in lactobacillus fluid medium;
C, under the condition of 30 DEG C ~ 40 DEG C and 0 ~ 200rpm, cultivate 1 ~ 5 day gained lactic acid bacteria culturers.
In technique scheme, carbon source containing lactobacillus normal fermentation in lactobacillus fluid medium described in step B, nitrogenous source, trace element and/or inorganic salts ingredients, wherein: carbon source composition is 0.5% ~ 10% of fluid medium gross weight, nitrogenous source composition is 0.5% ~ 3%, trace element and/or inorganic salts ingredients are 0.01% ~ 1%, all the other are water, sterilizing.Wherein said carbon source, nitrogenous source can select the conventional composition in current collective media, as conventional medium constituents such as glucose, sucrose, starch, Carnis Bovis seu Bubali cream, yeast extracts.Further preferred described sterilizing is through 115 ~ 121 DEG C of sterilizing 15 ~ 30min.
In technique scheme, solid medium described in steps A is made up of the lactobacillus fluid medium 1000ml described in step B and agar 10 ~ 20g, does conventional sterilant process by the same manner.Condition of culture is: under the 30-40 DEG C of condition with 0-200rpm, cultivate 1-3 days; Preferred condition of culture is: cultivate 1 day under 37 DEG C of conditions with 50rpm.
3rd technical problem solved by the invention is to provide the purposes of Radix Scutellariae lactobacillus fermented product of the present invention, be embodied in the activity that Radix Scutellariae lactobacillus fermented product of the present invention has obvious restraint of tyrosinase, melanic generation in check melanin oncocyte, suppresses the effect that main comedo pathogenic bacterium produce.Namely Radix Scutellariae lactobacillus fermented product of the present invention has the effect of clear and definite whitening, anti-acne control oil.Meanwhile, to human keratinized cell propagation unrestraint effect.
4th technical problem solved by the invention is to provide and adopts Radix Scutellariae lactobacillus fermented product of the present invention or its extract to be active component, adds the cosmetics that on cosmetics, acceptable adjuvant is prepared from.
Cosmetics prepared by the present invention can be the common cosmetics such as cosmetic water, emulsion, essence, facial cream, facial film, eye mask.
Radix Scutellariae lactobacillus fermented product of the present invention is adopted to be used for the nursing of skin, by the activity of restraint of tyrosinase, and melanic generation in check melanin oncocyte, and then play the effect of whitening, there is clear and definite white-skinned face function.Simultaneously, by " stripping property resists (pressing down) bacterium product bacteriostasis property test method ", working sample to comedo Main Pathogenic Bacteria (e.g., propionibacterium acnes, staphylococcus aureus) bacteriostasis rate, investigate obtain the effect with obvious anti-acne control oil.Meanwhile, by studying the inhibitory action that it is bred human keratinized cell, Radix Scutellariae lactobacillus fermented product can be obtained and not there is cytotoxicity as cosmetics use.The cosmetics that Radix Scutellariae lactobacillus fermented product of the present invention is prepared according to a conventional method have clear and definite whitening and anti-acne control oil effect.
Detailed description of the invention
The detailed description of the invention of form by the following examples, is described in further detail foregoing of the present invention again, illustrates but does not limit the present invention.
Embodiment 1 lactobacillus is on the growth adaptability of Radix Scutellariae-distilled water culture medium and impact test
(1) by 1.0 × 10
5lactobacillus is inoculated in Radix Scutellariae-distilled water culture medium (Radix Scutellariae-distilled water culture medium of 3%, 4%, 5%, 6%, 7% and 8% by cfu/ml inoculum concentration respectively, with Radix Scutellariae, distilled water for raw material, sterilizing after mixing, wherein all with Radix Scutellariae crude drug weight percent meter, below herewith), cultivate 2 ~ 4 days under 37 DEG C and static conditions, adopt the lactobacillus clump count in spread plate detection fermentation liquid.Result is as shown in table 1.
Table 1 result shows, and when the concentration of Radix Scutellariae is 3-8% in Radix Scutellariae-distilled water culture medium, all to a certain degree can promote the growth of lactobacillus; When in Radix Scutellariae-distilled water culture medium, the concentration of Radix Scutellariae is 5%, the growing way of lactobacillus is better and basically identical; When in Radix Scutellariae-distilled water culture medium, the concentration of Radix Scutellariae is greater than 5%, there is downward trend in the growth of lactobacillus; Therefore preferably adopt the concentration of Radix Scutellariae in Radix Scutellariae-distilled water culture medium to be 5%.
Table 1 ferments the impact that Radix Scutellariae concentration and fermentation time are cultivated lactobacillus first
Be it can also be seen that by table 1 result, when incubation time is 24 ~ 72 hours, the growth of lactobacillus is in exponential phase, fast growth; When incubation time is 72 ~ 96 hours, lactobacillus grow into decline phase, viable count in fermentation liquid reduces.Therefore, determine that the fermentation time of lactobacillus in the present invention is 72 hours.
(2) by 1.0 × 10
4, 1.0 × 10
5with 1.0 × 10
6lactobacillus is inoculated in the Radix Scutellariae-distilled water of 5% by cfu/ml inoculum concentration respectively, cultivates after 3 days under 37 DEG C and static conditions, adopts the lactobacillus clump count in spread plate detection fermentation liquid.Result is as shown in table 2.
The impact that table 2 inoculum concentration is cultivated lactobacillus
As can be seen from table 2 result, the viable count in fermentation liquid increases along with the increase of inoculum concentration, therefore, determines that the inoculum concentration of lactobacillus in the present invention is 1.0 × 10
6cfu/ml.
The whitening of embodiment 2 Radix Scutellariae lactobacillus fermented product of the present invention and anti-acne control oil efficacy assessments method and result
1, sample preparation
Radix Scutellariae lactobacillus fermented product prepare: by with above-mentioned by containing Radix Scutellariae 5% Radix Scutellariae-distilled water mixture in inoculating lactic acid bacterium 1.0 × 10
6cfu/ml, 37 DEG C, static conditions bottom fermentation removes liquid after 3 days, after rejoining distilled water 37 DEG C, the liquid that obtains for 2 days of static conditions bottom fermentation is test specimen.
Radix Scutellariae Aqueous extracts: Radix Scutellariae 500g, water extraction, concentrated, lyophilization, becomes test desired concn by sample dissolution before test.
Streptococcus acidi lactici fermented solution: inoculating lactic acid bacterium fermentation in 5L distilled water, fermentation condition prepares according to the process of Radix Scutellariae lactate fermentation condition, the homogenate 5L of gained fermented product through stirring, and 95 DEG C of heating are concentrated into 1L, filter, get filtrate, obtain fermented product stock solution.
The preparation of Radix Scutellariae extracting solution+lactic acid bacteria fermentation liquid mixture: Radix Scutellariae extracting solution and streptococcus acidi lactici fermented solution are mixed according to different proportion, concentrated, lyophilization, becomes test desired concn by sample dissolution before test.
2, the determination of Radix Scutellariae extracting solution and lactate fermentation liquid proportional
(1) test method
After Radix Scutellariae extracting solution and lactic fermentation liquid are mixed according to the ratio of 1:2,1:1,2:1, lyophilization, obtain three kinds of sample preparations are become the concentration of 4mg/mL, be index with Tyrosinase inhibition assays, determine the optimum proportioning of Radix Scutellariae extracting solution and lactic fermentation liquid.
Tryrosinase is the key enzyme in melanin synthesis process, directly affects melanic content.There is the activity of the material restraint of tyrosinase of whitening function, and the synthesis of check melanin, play the effect of whitening.
Phosphate buffer (pH6.8,0.1mol/L), sample liquid, tyrosine solution is added respectively in each test tube.Be placed in 37 DEG C of water-bath 10min, then add tryrosinase, then react 10min in 37 DEG C of water-baths, under 475nm, survey absorbance.Test system is in table 3, and C1 is blank, and C2 is used for blank zeroing, and T1 is sample cell, and T2 is used for sample zeroing.
Table 3 Tyrosinase inhibition assays system application of sample table
Tyrosinase inhibition rate (%)=(A0-A1)/A0 × 100%
A0: blank absorbance
A1: sample absorbance value
(2) result of the test, in table 3.
As can be seen from table 3 result of the test, when Radix Scutellariae extracting solution and lactic fermentation liquid proportioning are 1:1, the strongest to the inhibit activities of tryrosinase, with the proportioning test result of 1:2 and 2:1, there is significant difference.Known, best as the proportioning of follow-up test using 1:1.
3, external Tyrosinase inhibition assays
(1) test method
Test method is with the test method of the determination of Radix Scutellariae extracting solution and lactate fermentation liquid proportional " 2, " middle Tyrosinase inhibition assays.
(2) result of the test, in table 4.
Table 4 tyrosinase inhibition rate
From table 4 result of the test, the tyrosinase inhibitory activity of Radix Scutellariae lactobacillus fermented product strengthens along with the increase of concentration, its IC
50for 2.22mg/mL, action intensity is suitable with positive control arbutin.And compared with Radix Scutellariae extracting solution, lactic fermentation liquid and both mixed liquor samples, the effect of Radix Scutellariae lactobacillus fermented product is obviously better than former three, disclose the tunning obtained after Radix Scutellariae adopts lactate fermentation, create the material with stronger tyrosinase inhibitory activity, be developed as the cosmetics with whitening function and have a high potential.
4, melanic generation in check melanin oncocyte
(1) test method
B16 cell is by 2.0 × 10
4/ 400 Μ l/ holes, add in 24 orifice plates, and after cultivating 24h, add given the test agent, each sample 3 holes, sample final concentration is 5,10,20,40,80,160 μ g/Ml.Matched group replaces given the test agent with culture fluid.Hatch 72h, abandoning supernatant for 5%CO237 DEG C.The pancreatin adding 0.25% digests, and adds culture fluid and stops digestion, blow and beat into single cell suspension, get part Cell sap and carry out cell counting.After all the other cell suspension are centrifugal, supernatant discarded, adds 1M NaOH, and concussion 5min, goes out to measure absorbance in 490nm.
Suppression ratio (%)=[1-(sample well absorbance/sample well cell density)/(control wells absorbance/control wells cell density)] × 100%
(2) result of the test, in table 5.
Table 5 B16 cell suppression ratio
From result of the test, the inhibitory action of Radix Scutellariae lactobacillus fermented product to B16 cell strengthens along with the increase of concentration, its IC
50be 5 μ g/mL, action intensity and positive control arbutin are suitable, and during high concentration, to the suppression ratio of B16 cell higher than hydroquinone.And compared with Radix Scutellariae extracting solution, lactic fermentation liquid and both mixed liquor samples, the effect of Radix Scutellariae lactobacillus fermented product is obviously better than former three, disclose the tunning obtained after Ganoderma adopts Radix Scutellariae lactate fermentation, create the material had compared with high inhibition B16 cell.Illustrate that Radix Scutellariae lactobacillus fermented product has very strong whitening effect.Be raw material by Radix Scutellariae lactobacillus fermented product, can adopt its fermented product stock solution or fermented product powder, the cosmetics of the forms such as the cosmetic water prepared according to a conventional method, emulsion, essence, facial cream, facial film, eye mask also have clear and definite white-skinned face function.
5, the In Vitro Bacteriostasis of comedo Main Pathogenic Bacteria is studied
(1) test method
A. the preparation of culture medium: prepare culture medium according to a conventional method, adjust ph is that culture medium is added small test tube dress 1.8mL by 7.1 ~ 7.2., and plug seal, after 121 DEG C of autoclaving 30min, is placed in refrigerator (4 DEG C) and saves backup.
B. the preparation of bacteria suspension: by reference culture inoculated and cultured increasing bacterium respectively, under normal conditions (anaerobic jar cultivation) cultivation 24h, the dilution of absorption bacterium liquid culture medium is adjusted to 0.5 Maxwell and (is about 1.5 × 10 than turbid unit
8cFU/mg/ml), suspension culture medium is diluted, with hemocyte plate counting, suspension is adjusted to 1 × 10
6~ 10
5cFU/ml, for subsequent use.
C. the preparation of drug sensitive test pipe: with liquid-transfering gun add respectively in the small test tube that 1.8ml culture medium is housed 0.1 ~ 3.2mg/ml concentration of 20 μ l for reagent liquid, be mixed with the drug sensitive test pipe of a series of Concentraton gradient.Prepare control tube in addition: blank pipe (1.8ml culture medium), bacterial growth control pipe (1.8ml culture medium, 0.2ml bacteria suspension), Radix Scutellariae lactobacillus fermented product positive control pipe (1.8ml culture medium, 0.2ml bacteria suspension, 20 μ lDMF) positive control pipe (1.8ml culture medium, 0.2ml bacteria suspension, positive drug), observe the impact on bacterial growth of the growing state of antibacterial, solvent and medicine.
D. the inoculation of bacteria suspension: the pipet inoculation 0.2ml bacteria suspension after the sterilizing of all drug sensitive test pipe bacterium, is placed in anaerobic jar.48h is cultivated, observed result, with the MIC not occurring that muddy least concentration is said preparation under 37 DEG C of conditions.
(2) result of the test, table 6 and table 7.
Table 6 sample is to the MIC result of propionibacterium acnes
+: represent long bacterium ,-: represent not long bacterium
From result of the test, all blank pipes, drug-positive control tube culture medium are clarified, and illustrate that the growth of drug solvent 1%DNF solution on Bacteria does not affect, do not disturb result of the test.Table 6 result can be seen, the MIC of Radix Scutellariae lactobacillus fermented product to propionibacterium acnes is respectively 4 μ g/ml, 8 μ g/ml, 16 μ g/ml, 32 μ g/ml, the MIC of Radix Scutellariae extracting solution to propionibacterium acnes is respectively 16 μ g/ml, 32 μ g/ml, and the MIC of Radix Scutellariae extracting solution+lactic fermentation liquid to propionibacterium acnes is respectively 32 μ g/ml.Streptococcus acidi lactici fermented solution to propionibacterium acnes without bacteriostasis.
Table 7 sample is to the MIC result of staphylococcus aureus
+: represent long bacterium ,-: represent not long bacterium
Sample to the MIC result of staphylococcus aureus as can be seen from Table 7, the MIC of Radix Scutellariae lactobacillus fermented product to golden yellow Fructus Vitis viniferae bacillus is respectively 8 μ g/ml, 16 μ g/ml, 32 μ g/ml, Radix Scutellariae extracting solution and Radix Scutellariae extracting solution+lactic fermentation liquid are all 32 μ g/ml to the MIC of golden yellow Fructus Vitis viniferae bacillus, same lactic fermentation liquid to golden yellow Fructus Vitis viniferae bacillus without bacteriostasis.
6, human keratinized cell proliferation test is suppressed
(1) test method
Take the logarithm the Huke cell of phase, with trypsinization, dispel, prepare cell suspension, microscopic count, adjustment cell concentration, by 6 × 10
3individual/hole is inoculated into 96 orifice plates, 37 DEG C of overnight incubation.The sample of gradient dilution is added in drug treating group.Arrange blank group and positive controls (sodium lauryl sulphate (SDS)), each concentration does 3 repetitions simultaneously.Put into 37 DEG C, 5%CO
248h is cultivated in incubator.4h before off-test, by the culture fluid sucking-off in 96 orifice plates, adds 100 μ L PBS buffer and 10 μ L 5mg/mL MTT solution, hatches 4h for 37 DEG C.Add 100 μ L 10%SDS solution.37 DEG C of overnight incubation.Measure OD
570.Calculate suppression ratio.
Suppression ratio (%)=(sample sets OD
570-blank group OD
570) * 100/ blank group OD
570
Result of the test is as table 8, and the minimum or no cytotoxicity of the toxicity of four samples to horn cell, wherein Radix Scutellariae lactobacillus fermented product is the most obvious.
Table 8 sample is to human keratinized cell proliferative effect
" acute oral and acute dermal toxicity test " and " skin and acute ocular zest/corrosion test " during Radix Scutellariae lactobacillus fermented product safety testing adopts the cosmetic material passed through to detect, result of the test all shows that Radix Scutellariae lactobacillus fermented product belongs to nontoxic, nonirritant, non-corrosive cosmetic material, can be used in the formula of cosmetics.
Claims (10)
1. Radix Scutellariae lactobacillus fermented product, is characterized in that: with Radix Scutellariae and water composition culture medium, the then liquid fermentation mixture of inoculating lactic acid bacterium fermentation gained; Wherein, culture medium is with crude drug weighing scale, and Radix Scutellariae percentage by weight is 3 ~ 8%.
2. Radix Scutellariae lactobacillus fermented product according to claim 1, is characterized in that: culture medium is with crude drug weighing scale, and Radix Scutellariae percentage by weight is 5%.
3. Radix Scutellariae lactobacillus fermented product according to claim 1 and 2, is characterized in that: described Radix Scutellariae is primary crude drug or baikal skullcap root decoction pieces.
4. the Radix Scutellariae lactobacillus fermented product according to any one of claim 1-3, is characterized in that: in culture medium, the inoculum concentration of lactobacillus is 1.0 × 10
4~ 1.0 × 10
8cfu/ml; Preferably, in culture medium, the inoculum concentration of lactobacillus is 1.0 × 10
6cfu/ml.
5. the preparation method of Radix Scutellariae lactobacillus fermented product, is characterized in that: comprise the following steps:
A, with Radix Scutellariae and water composition culture medium, with crude drug weighing scale, Radix Scutellariae percentage by weight is 3 ~ 8%;
Inoculating lactic acid bacterium after B, sterilizing, inoculum concentration is 1.0 × 10
4~ 1.0 × 10
8cfu/ml;
C, cultivate under the condition of 30 DEG C ~ 40 DEG C and 0-200rpm after 24 ~ 72 hours and namely obtain Radix Scutellariae lactobacillus fermented product.
6. the preparation method of Radix Scutellariae lactobacillus fermented product according to claim 5, is characterized in that: at least meet following any one:
Culture medium is with crude drug weighing scale, and Radix Scutellariae percentage by weight is 5%;
The inoculum concentration of lactobacillus is 1.0 × 10
6cfu/ml;
" after sterilizing inoculating lactic acid bacterium " described in step B refers at 105 DEG C-115 DEG C sterilizing 20-40min, inoculates lactobacillus after cooling; Preferably, described " after sterilizing inoculating lactic acid bacterium " refers at 115 DEG C of sterilizing 30min, inoculates lactobacillus after cooling;
Step C cultivates 72 hours under the condition of 37 DEG C.
7. the preparation method of Radix Scutellariae lactobacillus fermented product according to claim 5, is characterized in that: the cultural method of lactic acid bacteria culturers comprises the steps:
A, in solid medium, cultivate lactic acid bacteria culturers;
B, by steps A cultivate lactobacillus inoculum enter in lactobacillus fluid medium;
C, under the condition of 30 DEG C ~ 40 DEG C and 0 ~ 200rpm, cultivate 1 ~ 5 day gained lactic acid bacteria culturers.
8. the preparation method of Radix Scutellariae lactobacillus fermented product according to claim 7, is characterized in that: at least meet following any one:
Carbon source containing lactobacillus normal fermentation in lactobacillus fluid medium described in step B, nitrogenous source, trace element and/or inorganic salts ingredients; Preferably, in culture medium, carbon source composition is 0.5% ~ 10% of fluid medium gross weight, and nitrogenous source composition is 0.5% ~ 3%, and trace element and/or inorganic salts ingredients are 0.01% ~ 1%, and all the other are water;
Solid medium described in steps A is made up of the lactobacillus fluid medium 1000ml described in step B and agar 10 ~ 20g;
Preferred steps A condition of culture is: under the 30-40 DEG C of condition with 0-200rpm, cultivate 1-3 days; Preferably condition of culture is further: cultivate 1 day under 37 DEG C of conditions with 50rpm.
9. Radix Scutellariae lactobacillus fermented product has in whitening, the anti-acne control oil medicine of effect or cosmetics in preparation and applies.
10. cosmetics, is characterized in that: with Radix Scutellariae lactobacillus fermented product described in any one of claim 1-4 or its extract for active component, add the cosmetics that on cosmetics, acceptable adjuvant is prepared from;
Preferably, described cosmetics are cosmetic water, emulsion, essence, facial cream, facial film, eye mask.
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| CN105420337A (en) * | 2015-12-16 | 2016-03-23 | 四川省中医药科学院 | Matsutake, caterpillar fungus and lactic acid bacteria fermented product as well as derivative cosmetics, preparation method and application thereof |
| CN106176556A (en) * | 2016-08-31 | 2016-12-07 | 张进 | A kind of removing acnes and controlling oil whitening cream |
| CN115607495A (en) * | 2022-11-15 | 2023-01-17 | 贺松林 | Whitening acne-removing mask and preparation method thereof |
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