CN106266229A - A kind of ferment with nourishing blood function and preparation method thereof - Google Patents

A kind of ferment with nourishing blood function and preparation method thereof Download PDF

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Publication number
CN106266229A
CN106266229A CN201610645434.6A CN201610645434A CN106266229A CN 106266229 A CN106266229 A CN 106266229A CN 201610645434 A CN201610645434 A CN 201610645434A CN 106266229 A CN106266229 A CN 106266229A
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parts
ferment
fructus
blood
raw material
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CN106266229B (en
Inventor
刘敬
李晓璐
赵斌
王琼
谢浩
麦健珍
蔡梦诗
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CHENGDU RUNXINTANG PHARMACEUTICAL Co.,Ltd.
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赵斌
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    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
    • A61K36/00Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18Magnoliophyta (angiosperms)
    • A61K36/185Magnoliopsida (dicotyledons)
    • A61K36/77Sapindaceae (Soapberry family), e.g. lychee or soapberry
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
    • A61K36/00Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18Magnoliophyta (angiosperms)
    • A61K36/185Magnoliopsida (dicotyledons)
    • A61K36/60Moraceae (Mulberry family), e.g. breadfruit or fig
    • A61K36/605Morus (mulberry)
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
    • A61K36/00Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18Magnoliophyta (angiosperms)
    • A61K36/185Magnoliopsida (dicotyledons)
    • A61K36/73Rosaceae (Rose family), e.g. strawberry, chokeberry, blackberry, pear or firethorn
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
    • A61K36/00Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18Magnoliophyta (angiosperms)
    • A61K36/185Magnoliopsida (dicotyledons)
    • A61K36/81Solanaceae (Potato family), e.g. tobacco, nightshade, tomato, belladonna, capsicum or jimsonweed
    • A61K36/815Lycium (desert-thorn)
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
    • A61K2236/00Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/10Preparation or pretreatment of starting material
    • A61K2236/19Preparation or pretreatment of starting material involving fermentation using yeast, bacteria or both; enzymatic treatment

Abstract

The invention discloses a kind of ferment with nourishing blood function, it is the raw material by following weight proportioning and microorganism is fermented is prepared from: Arillus Longan 240~360 parts, Fructus Mori 160~240 parts, Fructus Lycii 160~240 parts, Fructus Rubi 160~240 parts, yeast 0.04~0.16 part, lactic acid bacteria 1.4~5.4 parts.Efficacy experiments shows, Arillus Longan, Fructus Mori, Fructus Lycii and Fructus Rubi four taste raw material can play synergistic function under the specific consumption proportion of the present invention, have significant nourishing blood function.It addition, the raw material of above-mentioned four kinds of integration of edible and medicinal herbs is prepared as the ferment with nourishing blood function by the present invention, compared with raw material, mouthfeel is more preferable, takes more convenient, and the beneficiating ingredient such as extracellular polysaccharide, lactic acid bacteria dramatically increases, and add SOD activity, there is more preferable health-care effect.

Description

A kind of ferment with nourishing blood function and preparation method thereof
Technical field
The present invention relates to a kind of ferment with nourishing blood function and preparation method thereof, belong to field of medicaments.
Background technology
Blood deficiency belongs to Chinese medical disease category, refers to hematopenia, the nutrition of blood and moist hypofunction, so that internal organs warp Network, body organ etc. lose support pathological state, clinically with pale white complexion or sallow, hair is the dampest, words onyx is light white, head Dizzy, blurring of vision, severe palpitation, cardiopalmus, forgetful, insomnia, weak etc. be mainly to show.Syndrome of deficiency of blood is on doctor trained in Western medicine ill-distribution, not only See anemia, be also shown in other diseases, such as coronary heart disease, neurosis, viral myocarditis etc..
Modern study confirms, blood deficiency may result in the going down of hemopoietic function of bone marrow and peripheral red blood cells content and function, exempts from Epidemic disease function reduction, body Scavenging ability reduce, and the disorder of multiple hormone secretion even occur, affects fertility etc. sternly Weight consequence, very harmful (Guo's equality. syndrome of deficiency of blood pathological study progress [J]. Shandong journal of Chinese medicine, 2006,25 (9): 643~ 645).Owing to the pathogenesis of blood deficiency is complicated, often with multiple systems, the pathological change of organ, the big multiple-effect of existing treatment means Fruit is the best, and some medicines also have obvious untoward reaction.Such as, for iron deficiency anemia, the most often give iron Agent is treated;But, the ferrum major part that human body is taken in is difficult to be fully used, and can be absorbed by organisms and play the most on a small quantity Physiological function, adds iron preparation and often has intestines and stomach stimulation, more hinders the absorption of medicine, treats anemia effect Little.
Therefore it provides one has significant nourishing blood function, and without medicine, health product or the food of obvious toxic-side effects, Become a problem demanding prompt solution.
Ferment refers to one or more fresh vegetabless, fruit, mushroom, Chinese herbal medicine etc. as raw material, ferments through multiple beneficial bacterium And produce, the functional microbial fermentation containing nutritional labelings such as abundant vitamin, enzyme, mineral and secondary metabolites Product.Ferment not only saves nutrient substance intrinsic in fermentation raw material, and it is more original to improve fermentation raw material Bad flavor, it is often more important that create some new functional components, the health-care effect the best to human body during the fermentation.
But, fermentable is a complicated process, affects the many factors of ferment product quality, such as, fermentation The technological parameter such as strain, inoculum concentration is particularly evident on the impact of ferment quality, determines suitable fermentation condition, thus obtains one Excellent flavor and the higher ferment product of content of functional component are the most difficult.
Summary of the invention
It is an object of the invention to provide a kind of ferment with nourishing blood function and preparation method thereof.
The invention provides a kind of ferment with nourishing blood function, it is the raw material by following weight proportioning and microorganism warp Fermentation is prepared from: Arillus Longan 240~360 parts, Fructus Mori 160~240 parts, Fructus Lycii 160~240 parts, Fructus Rubi 160~240 Part, yeast 0.04~0.16 part, lactic acid bacteria 1.4~5.4 parts.
Preferably, it is the raw material by following weight proportioning and microorganism is fermented is prepared from: Arillus Longan 300 parts, Mulberry Mulberry 200 parts, Fructus Lycii 200 parts, Fructus Rubi 200 parts, 0.1 part of yeast, lactic acid bacteria 3 parts.
It is further preferred that described lactic acid bacteria is selected from Lactobacillus bulgaricus, streptococcus thermophilus, bacillus acidophilus, green grass or young crops One or more in spring bacillus bifidus.
The invention provides the preparation method of a kind of described ferment, comprise the steps:
A, weigh the raw material of each weight proportion, crushing and beating, pectinase enzymatic hydrolysis, isolated enzymolysis solution, enzyme denaturing, sterilizing, Cooling;
B, enzymolysis solution through yeast fermentation, isolated supernatant, standby;
Inoculating lactic acid bacterium in c, supernatant, second time fermentation, fermentation liquid is standby;
D, fermentation liquid are centrifugal, take supernatant, obtain ferment stock solution.
Further, the condition of described pectinase enzymatic hydrolysis is: regulation pH to 3~4, under the conditions of 45~50 DEG C, by weight Than addition 0.2~the pectase of 0.5%, enzymolysis 2~4 hours.
Further, the condition of described yeast fermentation is: fermentation temperature is 20~30 DEG C, ferments 24~72 hours.
Further, the condition of described second time fermentation is: fermentation temperature is 30~35 DEG C, ferments 12~36 hours.
The invention provides described ferment, in preparation, there is the purposes in the medicine of nourishing blood function, food or health product.
Further, described medicine, health product or food has treatment and/or effect of prevention blood deficiency.
Further, described medicine, health product or food has treatment and/or prevention Lost blood anemia, chemical blood Empty effect.
The invention provides a kind of ferment with nourishing blood function.This zymogen material prescription is reasonable, reuses the dragon of sweet temperature Eye meat is monarch, and invigorating the heart and spleen, nourishing blood to tranquillize the mind, for insufficiency of vital energy and blood, severe palpitation, forgetful insomnia, blood deficiency and yellow complexion;Minister with Fructus Mori, Sweet acidity is trembled with fear, and enters Liver and kidney warp, nourishing YIN and benefiting blood, promotes the production of body fluid, intestine moistening.The having a dizzy spell of main deficiency of the liver and kindey and blood deficiency damage of essence, soreness of waist tinnitus, Early whitening of beard and hair, insomnia and dreamful sleep, Tianjin wound are thirsty, quench one's thirst, dryness of the intestine constipation.Arillus Longan strongly invigorating QI and blood, Fructus Mori nourishing YIN and benefiting blood, two medicines close With, it is intended to negative and positive of qi and blood is with mending;And a Wen Yihan, clothes are the most scorching, again without the fraud of deficiency and coldness for a long time.Help with sweet flat Fructus Lycii, grow Invigorating the liver and kidney, replenishing vital essence to improve eyesight;Help with sweet acid, the Fructus Rubi of temperature, kidney tonifying, controlling nocturnal emission with astringent drugs, reducing urination.Four tastes share, and have both mended congenital kidney, have mended again The spleen of the day after tomorrow;Both reinforcing the heart, the blood of liver, the moon of invigorating the liver and kidney, and coordination of cold and heat again, negative and positive of qi and blood is taken into account, and tonification takes into account solid simultaneously Puckery, strengthen the strength of tonification.
Efficacy experiments shows, Arillus Longan, Fructus Mori, Fructus Lycii and Fructus Rubi four taste raw material are under the specific consumption proportion of the present invention Synergistic function can be played, there is significant nourishing blood function.
It addition, the raw material of above-mentioned four kinds of integration of edible and medicinal herbs is prepared as the ferment with nourishing blood function by the present invention, with raw material Comparing, mouthfeel is more preferable, takes more convenient, and the beneficiating ingredient such as extracellular polysaccharide, lactic acid bacteria dramatically increases, and adds SOD activity, There is more preferable health-care effect.
Obviously, according to the foregoing of the present invention, according to ordinary technical knowledge and the customary means of this area, without departing from Under the present invention above-mentioned basic fundamental thought premise, it is also possible to make the amendment of other various ways, replace or change.
The detailed description of the invention of form by the following examples, remakes the most specifically the foregoing of the present invention Bright.But this should not being interpreted as, the scope of the above-mentioned theme of the present invention is only limitted to Examples below.All based on foregoing of the present invention The technology realized belongs to the scope of the present invention.
Detailed description of the invention
The raw material, the equipment that use in the specific embodiment of the invention are known product, obtain by buying commercially available prod.
The preparation method of embodiment 1 ferment of the present invention
Weigh Arillus Longan 150g, Fructus Mori 100g, Fructus Lycii 100g, Fructus Rubi 100g, remove impurity, clean, add 2~4 After times amount boiling sterilization, the pure water of cooling, soaks 30 minutes, crushing and beating;
Regulation pH to 3~4, under the conditions of 45~50 DEG C, by weight addition 0.2~the pectase of 0.5%, enzymolysis 2~4 Hour, separate extracting juice, boil 10 minutes, sterilizing, be cooled to room temperature;
In above-mentioned enzymolysis solution, add yeast 0.05g, maintenance fermentation temperature 20~30 DEG C, ferment 24~72 hours, point From supernatant, standby;
In above-mentioned supernatant, inoculating lactic acid bacterium (Lactobacillus bulgaricus, streptococcus thermophilus, bacillus acidophilus, youth pair One or several mixed vaccine in discrimination bacillus) 1.5g, maintenance fermentation temperature 30~35 DEG C, ferment 12~36 hours, standby;
Centrifugal, take supernatant, fill, preserve under the conditions of 4 DEG C, obtain the ferment stock solution of the present invention.
Beneficial effects of the present invention is proved below by way of experimental example.
The effect of enriching blood of experimental example 1 raw material of the present invention
1, the preparation of test solution
Enrich blood formula extraction: take Arillus Longan 30g, Fructus Mori 20g, Fructus Lycii 20g, Fructus Rubi 20g, add 2 times of volume water loggings Steeping 30 minutes, every time with 6 times of volume water, decoct 2 times, each 30 minutes, collecting decoction, filtration, the every 1ml of concentrating under reduced pressure contained 1g The concentration of crude drug amount.
Arillus Longan extract: taking Arillus Longan 90g, ibid prepared by method,.
Fructus Mori extract: taking Arillus Longan 90g, ibid prepared by method,.
Fructus Lycii extract: taking Fructus Lycii 90g, ibid prepared by method,.
Fructus Rubi extract: taking Fructus Rubi 90g, ibid prepared by method,.
2, the research on the impact of Lost blood anemia mice
Take mice (Kunming kind, one-level, male and female half and half, body weight 18~22g) 56, male and female half and half, be randomly divided into 7 groups, point Do not gavage formula extraction of enriching blood (1.35g/kg), Arillus Longan extract (1.35g/kg), Fructus Mori extract (1.35g/kg), Fructus Lycii Seed extract (1.35g/kg), Fructus Rubi extract (1.35g/kg) or same volume normal saline, continuous 7 days, in the 7th be administered Day retroorbital venous blood-letting about 0.6ml, causes mice to lose blood model, Normal group not blood-letting.Continue after losing blood to be administered 7 days, After last is administered 1.5h, eye socket takes blood, anticoagulant heparin, measures mice RBC, Hb value with Automatic blood cell analyzer.Measurement result It is shown in Table 1.
Result of study that Lost blood anemia mice is affected by table 1 present composition (N=8)
Group RBC(1012/L) Hb(g/L)
Enrich blood formula extraction 8.72±0.73** 151.05±9.03*
Arillus Longan extract 8.14±0.75** 148.03±9.82
Fructus Mori extract 8.25±0.86** 147.88±12.86*
Fructus Lycii extract 5.33±0.88 130.84±11.28
Fructus Rubi extract 5.22±0.87 137.02±13.08
Normal control 9.27±0.51** 151.23±9.02*
Model comparison 5.28±0.87 132.26±17.21
Note: compare with model control group, * P < 0.05, * * P < 0.01
Experimental result: occur being slow in action after mice blood-letting modeling, dyspnea with rapid and short breath, hair is fluffy perpendicular and lacks gloss, and eye ear, tail are pale Blood deficiency symptom;Comparing with normal group, model group erythrocyte number and content of hemoglobin are all remarkably decreased, prompting modeling success.
The present invention side of enriching blood group mice physical agility, hair color is normal, with normal group mice without being clearly distinguished from;With model group ratio Relatively, the present invention side of enriching blood group erythrocyte number and content of hemoglobin are significantly increased, and show that the present composition is to blood loss Blood deficiency has obvious therapeutical effect.
It addition, compared with model group, it is red that alone Fructus Lycii extract or Fructus Rubi extract can not be effectively improved mice Cell quantity or content of hemoglobin;Alone Arillus Longan extract or Fructus Mori extract, mouse red blood cell quantity or hemoglobin Content is also below the present invention side of enriching blood group.
Result above shows, the present invention four taste raw material has played synergistic function, has and significantly treats blood loss blood Empty effect.
3, the research on the impact of chemical blood deficiency mice
Take mice (Kunming kind, one-level, male and female half and half, body weight 18~22g) 56, male and female half and half, be randomly divided into 7 groups, with Upper administration, in the 3rd day, subcutaneous injection acetylphenylhydrazine 20mg/kg, 40mg/kg on the 6th being administered, abdominal cavity note every day from the 3rd day Penetrating cyclophosphamide 40mg/kg continuous 5 days, after last is administered 1.5h, eye socket takes blood, anticoagulant heparin, analyzes with automatic blood cell Instrument measures WBC, RBC, Hb, HCT value.Mice, weighed spleen weight is put to death after taking blood.Measurement result is shown in Table 2.
Result of study that chemical blood deficiency mice is affected by table 2 present composition (N=8)
Note: compare with model control group, * P < 0.05, * * P < 0.01
Experimental result: mice, after acetylphenylhydrazine, cyclophosphamide combined modeling, starts being slow in action occur, breathe heavily on the 4th Promote, hair is fluffy perpendicular and few gloss, the blood deficiency symptoms such as eye, ear, tail are pale, the group mice change of the present invention side of enriching blood not quite, physical agility, Hair color is normal, with normal group mice without being clearly distinguished from.Each test group of animals is all without dead.
Model group compares with normal group, RBC number, leukocyte count, content of hemoglobin, hematocrit value and spleen weight Amount is all remarkably decreased.Compare with model group, the present invention side of enriching blood group RBC number, leukocyte count, content of hemoglobin, erythrocyte Specific volume and spleen weight are significantly increased, and show that the present invention has the bone marrow depression of antagonism caused by cyclophosphamide, promotes hematopoietic function Effect.
It addition, compared with alone Fructus Lycii extract, Fructus Rubi extract, Arillus Longan extract or Fructus Mori extract, this The invention side of the enriching blood group above-mentioned indices of mice is in optimum level, shows that four taste raw materials have played synergistic function, tool There is effect of significant therapeutical chemistry blood deficiency.
The quality evaluation of experimental example 2 ferment of the present invention
The ferment of the present invention prepared according to embodiment 1 is carried out quality evaluation, the results are shown in Table 3.
Table 3 ferment of the present invention quality evaluation result
* note: % refers to g/100ml.
Measurement result shows, ferment of the present invention is compared with raw material, and mouthfeel is more preferable, takes more convenient, extracellular polysaccharide, lactic acid bacteria Dramatically increase Deng beneficiating ingredient, and add SOD activity.

Claims (10)

1. there is a ferment for nourishing blood function, it is characterized in that: it be the raw material by following weight proportioning and microorganism fermented It is prepared from: Arillus Longan 240~360 parts, Fructus Mori 160~240 parts, Fructus Lycii 160~240 parts, Fructus Rubi 160~240 parts, ferment Female bacterium 0.04~0.16 part, lactic acid bacteria 1.4~5.4 parts.
2. ferment as claimed in claim 1, is characterized in that: it is the raw material by following weight proportioning and the fermented system of microorganism For forming: Arillus Longan 300 parts, 200 parts of Fructus Mori, Fructus Lycii 200 parts, Fructus Rubi 200 parts, 0.1 part of yeast, lactic acid bacteria 3 parts.
3. ferment as claimed in claim 1 or 2, is characterized in that: described lactic acid bacteria is selected from Lactobacillus bulgaricus, thermophilic chain One or more in coccus, bacillus acidophilus, bifidobacterium adolescentis.
4. a preparation method for ferment described in claims 1 to 3 any one, is characterized in that: comprise the steps:
A, weigh the raw material of each weight proportion, crushing and beating, pectinase enzymatic hydrolysis, isolated enzymolysis solution, enzyme denaturing, sterilizing, cooling;
B, enzymolysis solution through yeast fermentation, isolated supernatant, standby;
Inoculating lactic acid bacterium in c, supernatant, second time fermentation, fermentation liquid is standby;
D, fermentation liquid are centrifugal, take supernatant, obtain ferment stock solution.
5. preparation method as claimed in claim 4, is characterized in that: the condition of described pectinase enzymatic hydrolysis is: regulation pH to 3~4, Under the conditions of 45~50 DEG C, by weight addition 0.2~the pectase of 0.5%, enzymolysis 2~4 hours.
6. preparation method as claimed in claim 4, is characterized in that: the condition of described yeast fermentation is: fermentation temperature is 20 ~30 DEG C, ferment 24~72 hours.
7. preparation method as claimed in claim 4, is characterized in that: the condition of described second time fermentation is: fermentation temperature is 30 ~35 DEG C, ferment 12~36 hours.
8. ferment described in claims 1 to 3 any one has the use in the medicine of nourishing blood function, food or health product in preparation On the way.
9. purposes as claimed in claim 8, is characterized in that: described medicine, health product or food has treatment and/or prevention Effect of blood deficiency.
10. purposes as claimed in claim 9, is characterized in that: described medicine, health product or food has treats and/or pre- Anti-Lost blood anemia, effect of chemical blood deficiency.
CN201610645434.6A 2016-08-08 2016-08-08 Enzyme with blood replenishing effect and preparation method thereof Active CN106266229B (en)

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Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106822601A (en) * 2017-02-07 2017-06-13 中山火炬职业技术学院 A kind of dendrobium candidum ferment Ultramicro-powder and preparation method thereof
CN106962925A (en) * 2017-03-30 2017-07-21 程君 Make the composition and its method of ferment
CN107997150A (en) * 2018-01-25 2018-05-08 福建新味食品有限公司 A kind of processing method of vegetables and fruits enzyme liquid and application
CN108887674A (en) * 2018-07-10 2018-11-27 集美大学 A kind of preparation method of lycium ruthenicum composite enzyme liquid
CN109907307A (en) * 2017-12-13 2019-06-21 山东博奥克生物科技有限公司 A kind of mulberry enzyme
CN110089744A (en) * 2019-06-05 2019-08-06 重庆康菌泰生物科技股份有限公司 A kind of raspberry comprehensive enzyme and preparation method thereof

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Publication number Priority date Publication date Assignee Title
CN102399658A (en) * 2010-09-16 2012-04-04 吴刚 Health fruit wine with functions of blood nourishing and face beautifying
CN104522783A (en) * 2014-12-30 2015-04-22 北海万物盛生物技术开发有限公司 Chinese wampee enzyme drink and preparation method thereof

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102399658A (en) * 2010-09-16 2012-04-04 吴刚 Health fruit wine with functions of blood nourishing and face beautifying
CN104522783A (en) * 2014-12-30 2015-04-22 北海万物盛生物技术开发有限公司 Chinese wampee enzyme drink and preparation method thereof

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106822601A (en) * 2017-02-07 2017-06-13 中山火炬职业技术学院 A kind of dendrobium candidum ferment Ultramicro-powder and preparation method thereof
CN106962925A (en) * 2017-03-30 2017-07-21 程君 Make the composition and its method of ferment
CN109907307A (en) * 2017-12-13 2019-06-21 山东博奥克生物科技有限公司 A kind of mulberry enzyme
CN107997150A (en) * 2018-01-25 2018-05-08 福建新味食品有限公司 A kind of processing method of vegetables and fruits enzyme liquid and application
CN108887674A (en) * 2018-07-10 2018-11-27 集美大学 A kind of preparation method of lycium ruthenicum composite enzyme liquid
CN110089744A (en) * 2019-06-05 2019-08-06 重庆康菌泰生物科技股份有限公司 A kind of raspberry comprehensive enzyme and preparation method thereof

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