CN102746974B - Cordyceps sinensis health wine and preparation method thereof - Google Patents
Cordyceps sinensis health wine and preparation method thereof Download PDFInfo
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Mushroom Cultivation (AREA)
Abstract
The invention provides a cordyceps sinensis health wine which is prepared from the fermentation stock liquid of cordyceps sinensis mycelia, the extraction liquid of traditional Chinese medicines and 58-62 DEG original basic white spirit prepared from pure grains, wherein the fermentation stock liquid of cordyceps sinensis mycelia is prepared by performing sterile body filtration on cordyceps sinensis strains through the deep liquid fermentation technology; and the extraction liquid of traditional Chinese medicines is prepared by extracting dwarf lilyturf tubers, radix polygonati officinalis, pulp of dogwood fruits, cistanche and epimedium herbs. The invention also provides a preparation method of the cordyceps sinensis health wine. The fermentation stock liquid of cordyceps sinensis mycelia adopted in the formula is a whole fermentation liquid mixed with the stock liquid of fermented thallis by using the specific fermentation technology, cells are broken, and the fermentation stock liquid of cordyceps sinensis mycelia is concentrated and compounded with a plurality of traditional Chinese medicines to prepare. The cordyceps sinensis health wine not only can reserve the effective ingredients of natural cordyceps sinensis and play the medical and health-care actions, but also can control production, the yield is increased, the cost is reduced, and the cordyceps sinensis health wine is applicable to popularization.
Description
Technical field
The utility model relates to a kind of wine with nourishing function, particularly a kind of aweto health-care wine and preparation method thereof.
Background technology
China people just have a deep understanding to the medical care effect of Chinese caterpillar fungus health-care a long time ago, think that Chinese caterpillar fungus health-care has the effect of " protecting lung benefit liver, supplying vital essence and marrow, hemostasis and phlegm ", " can control hundred and need hundred damages ".Modern medicine study has further been verified the medical health care function of Chinese caterpillar fungus health-care from aspects such as pharmacology and clinical efficacies, through modern medicine study, prove, Cordyceps sinensis contains multiple health care composition, wherein crude protein 25.32%, fat 8.4%, robust fibre 18.59%, carbohydrate 28.9%, ash content 4.1%, Chinese caterpillar fungus health-care acid 7%, the every 100g of vitamin B12, containing 29 micrograms, is also rich in indolylacetic acid, the multiple nutrients materials such as the amino acid of needed by human, trace element, Chinese caterpillar fungus health-care polysaccharide, superoxide-dismutase (SOD).
Often edible Cordyceps sinensis can improve immune function of human body, and can be relieving cough and reducing sputum, treatment pulmonary tuberculosis, and worn with age, anaemia is weak, nervosa stomach trouble, soreness of waist and knee joint, impotence and seminal emission, void does not wait disease again for a long time after being ill.Modern medicine man finds, in addition anticancer, antifatigue, antiviral, hypotensive, improve the functions such as cardiac and cerebral blood-supply, liver cirrhosis and Growth of Nasopharyngeal Carcinoma are had to significant restraining effect.
At present, to prepare similar healthcare products kind a lot of as basic raw material to take Cordyceps sinensis etc., but generally all select the sporophore of natural cordyceps health care and mushroom, glossy ganoderma, after pulverizing, mix; Or carry out solid fermentation, together with substratum dry, pulverize, selection, granulation, packing; Or carry out liquid submerged fermentation, thalline is filtered out to reconcentration fermented liquid.Before a kind ofly because natural resource are limit, cause and yield poorly, cost is high, is difficult to apply, and meets the need of market.The more original backwardness of the second solid fermentation, though can reduce certain cost, because of can not scale production, and easily pollutes, and causes loss, is also eliminated gradually.The third deep fermentation, due to thalline is filtered out, affects some active ingredient and feature that natural matter has.
Summary of the invention
Technical problem to be solved by this invention is to provide and a kind ofly both can keeps natural cordyceps effective ingredient, and can play aweto health-care wine of containing of special medical health-care effect a large amount of broken wall mycelium polymkeric substance and preparation method thereof.
In order to solve the problems of the technologies described above, the invention provides a kind of aweto health-care wine, be to adopt Cordyceps mycelium fermenation raw liquid, extracts of Chinese herbal medicine and the former base white wine of 58～62 pure grain brew of degree formulated.
Described Cordyceps mycelium fermenation raw liquid is that the deep fermentation technology that Cordyceps strain is carried out filtering without thalline is made.
Described extracts of Chinese herbal medicine is to be extracted and made by the tuber of dwarf lilyturf, radix polygonati officinalis, Fructus Corni, Herba Cistanches and Herba Epimedii.
The weight percent of described Cordyceps mycelium fermenation raw liquid, extracts of Chinese herbal medicine and the former base white wine of 58～62 pure grain brew of degree is 0.8～1:0.05～0.08:1.6～1.8.
The present invention also provides the preparation method of above-mentioned aweto health-care wine, comprises the steps:
Step 1. the preparation of Cordyceps mycelium fermenation raw liquid
A. the cultivation of Cordyceps sinensis slant strains
Get natural cordyceps and carry out separate tissue, on slant medium, cultivate, make slant strains;
B. the cultivation of liquid spawn
Get in slant strains liquid medium within and cultivate, make liquid spawn;
C. submerged fermentation enlarged culturing
By liquid spawn under aseptic condition successively access fill in one-level through the nutrient solution of sterilising treatment, secondary, three grades of submerged fermentation incubators, through cultivating, obtain live body bacterium liquid;
D. active somatic cells broken wall
Live body bacterium liquid, through colloidal mill and high pressure homogenizer fracturing cell walls, is formed to suspendible glue, and at 32～42 ℃, insulation is placed 1～2 hour, and intracellular matter is fully oozed out, and obtains Cordyceps mycelium fermenation raw liquid;
Step 2. the preparation of extracts of Chinese herbal medicine
Take the Chinese medicine material of following weight: the tuber of dwarf lilyturf 30～45g, radix polygonati officinalis 30～45g, Fructus Corni 30～50g, Herba Cistanches 25～45g, Herba Epimedii 15～30g, the water extraction secondary by above-mentioned Chinese medicine material with 10 times of amounts, filters to obtain extracts of Chinese herbal medicine, extracting is for the first time to extract 120 minutes at 90～110 ℃ of temperature, and extracting is for the second time at 90～110 ℃, to extract 60 minutes.
Step 3: obtain finished product after blending the former base white wine of the pure grain brew of 60 degree
The canned acquisition finished product of homogeneous after active substance in the above-mentioned Cordyceps mycelium fermenation raw liquid making, extracts of Chinese herbal medicine and the former base white wine of the pure grain brew of 60 degree are merged.
The substratum that the cultivation of the slant strains in described step 1 adopts is PDA substratum, and culture condition for to cultivate 5～7 days at 23～25 ℃.
Composition and the weight of the liquid nutrient medium that the cultivation of the liquid spawn in described step 1 is used are as follows: 180～220 grams, potato, 18～22 grams of glucose, 2～4 grams of peptones, 0.5～1.5 gram of yeast extract paste, 0.4～0.6 gram, sal epsom, 0.5～1.5 gram of potassium primary phosphate, culture condition is nature pH value, at 23～25 ℃, incubation time is 36～42 hours.
Composition and the weight of the substratum that submerged fermentation enlarged culturing in described step 1 is used are respectively: millet 20～25g, glucose 10～15g, white sugar 2～5g, potassium dihydrogen sulfate 0.5～1.8g, sal epsom 0.1～0.25g, peptone 5～8g, yeast extract 1～2g, amino acid 0.1～0.2g, vitamin V B1 0.01g, adds water to 1000ml.
The culture condition of the submerged fermentation enlarged culturing in described step 1 is: with 10% inoculum size access liquid spawn, at 23-25 ℃, in ventilating ratio 1:0.5～0.7 and cultivation, press under the condition for 0.05MPa, 36-38 hour continuously ferments, now PH is 3.8～4.2, mycelium natural subsidence 100%.
In the extracts of Chinese herbal medicine that aweto health-care wine of the present invention is used, used raw material is mainly used in nourishing Yin and promoting production of body fluid the tuber of dwarf lilyturf, the effect that moistening lung clears away heart-fire, cures mainly that dryness of the lung dry cough, consumptive disease cough, thirsty, the vexed insomnia of Tianjin wound, interior heat are quenched one's thirst, the dry constipation of intestines and pharyngeal diphtheria; And radix polygonati officinalis has moistening lung enriching yin, the effect of nourishing the stomach to promote the production of body fluid, cures mainly cough caused by dryness-heat, and consumptive disease is coughed for a long time; Consumption of YIN caused by febrile disease is thirsty, and interior heat is quenched one's thirst; Deficiency of Yin diseases caused by external factors, fever and chills nasal obstruction; The head is giddy, muscle arteries and veins contraction pain; That Fructus Corni can tonify the liver and kidney, restrain is astringent or styptic treatment for spontaneous sweating, controlling nocturnal emission with astringent drugs contracting urine, only band only collapses, hidroschesis, promotes the production of body fluid to quench thirst, and can be used for menoxenia, profuse sweating collapse and interior heat under soreness of waist and knee joint, dizziness and tinnitus, forgetful, emission and spermatorrhea, enuresis frequent micturition, uterine bleeding band and quenches one's thirst; Herba Cistanches can kidney-replenishing, and benefiting essence-blood, relaxes bowel, for impotence, infertile, soreness of the waist and knees, and muscles and bones is unable, the dry constipation of intestines.Kidney-replenishing, strengthening the bones and muscles, the diseases such as wind-damp dispelling; Herba Epimedii is soft for impotence and seminal emission, muscles and bones impotence, rheumatic arthralgia, numbness contracture and climacteric hypertension.The present invention adopts Cordyceps mycelium fermenation raw liquid, extracts of Chinese herbal medicine and the former base white spirit mixing of the 60 pure grain brew of degree finished product, wherein Cordyceps mycelium fermenation raw liquid is to use the deep fermentation technology of filtering without thalline, the whole beer that the thalline stoste that obtains fermenting is lumped together, carry out again cytoclasis, concentrating and be equipped with several Chinese medicine modulates, in its process, retain a large amount of broken wall mycelium polymkeric substance and formed suspendible body finished product, both can keep natural cordyceps effective ingredient, play special medical health-care effect, again can production control, improve output, reduce costs, be applicable to promoting.
Embodiment
The invention provides a kind of aweto health-care wine, is to adopt Cordyceps mycelium fermenation raw liquid, extracts of Chinese herbal medicine and the former base white wine of the 60 pure grain brew of degree formulated.
The preparation method of described aweto health-care wine, comprises the steps:
Step 1. prepare Cordyceps mycelium fermenation raw liquid
A. the cultivation of Cordyceps sinensis slant strains
Get natural cordyceps and carry out separate tissue, on slant medium, cultivate, make slant strains; The substratum that slant culture adopts is PDA substratum, cultivates 5～7 days at 23～25 ℃.
B. the preparation of liquid spawn
Get in the inclined-plane solid spawn liquid medium within of above-mentioned cultivation and cultivate, make liquid spawn; Wherein composition and the weight of liquid nutrient medium (in 1000ml) are as follows: 180～220 grams, potato, 18～22 grams of glucose, 2～4 grams of peptones, 0.5～1.5 gram of yeast extract paste, 0.4～0.6 gram, sal epsom, 0.5～1.5 gram of potassium primary phosphate, natural pH value, at 23～25 ℃, incubation time is 36～42 hours.
C. submerged fermentation enlarged culturing
By liquid spawn under aseptic condition successively access fill in one-level through the nutrient solution of sterilising treatment, secondary, three grades of submerged fermentation incubators, through cultivating, obtain live body bacterium liquid.
Wherein fermention medium (substratum of one-level, secondary, three grades of submerged fermentations is all identical) composition and weight are: millet 20～25g, glucose 10～15g, white sugar 2～5g, potassium dihydrogen sulfate 0.5～1.8g, sal epsom 0.1～0.25g, peptone 5～8g, yeast extract 1～2g, amino acid 0.1～0.2g, vitamin V B1 0.01g, adds water to 1000ml.
Its process following (condition of one-level, secondary, three grades of submerged fermentations is all identical): with 10% inoculum size access liquid spawn, at 23-25 ℃, in ventilating ratio 1:0.5～0.7 and cultivation, press under the condition for 0.05MPa, 36-38 hour continuously ferments, now PH is 3.8～4.2, mycelium natural subsidence 100%.
D. active somatic cells broken wall
Live body bacterium liquid is ground to (3T/H) through colloidal mill, then use high pressure homogenizer (3T/H, processing pressure 40MPa) fracturing cell walls, form suspendible glue, at 32～42 ℃, insulation is placed 1～2 hour, and intracellular matter is fully oozed out, and obtains Cordyceps mycelium fermenation raw liquid.
Step 2. prepare extracts of Chinese herbal medicine
Take the Chinese medicine material of following weight: the tuber of dwarf lilyturf 30～45g, radix polygonati officinalis 30～45g, Fructus Corni 30～50g, Herba Cistanches 25～45g, Herba Epimedii 15～30g, the water extraction secondary by above-mentioned Chinese medicine material with 10 times of amounts, filters to obtain extracts of Chinese herbal medicine, extracting is for the first time to extract 120 minutes at 100 ℃ of temperature, and extracting is for the second time at 100 ℃, to extract 60 minutes.
Step 3: obtain finished product after blending the former base white wine of the pure grain brew of 60 degree
By the above-mentioned Cordyceps mycelium fermenation raw liquid making, extracts of Chinese herbal medicine and the former base white wine of the 60 pure grain brew of degree, according to mass ratio, be the canned acquisition suspendible of homogeneous body finished product after 0.8～1:0.05～0.08:1.6～1.8 merge.
Now the present invention is further described in conjunction with the embodiments, but the invention is not restricted to following examples.
Embodiment 1
The present embodiment provides a kind of preparation method of aweto health-care wine, comprises the steps:
Step 1. prepare Cordyceps mycelium fermenation raw liquid
A. the cultivation of Cordyceps sinensis slant strains
Get natural cordyceps and carry out separate tissue, on slant medium, cultivate, make slant strains; The substratum that slant culture adopts is PDA substratum, cultivates 6 days at 25 ℃.
B. the preparation of liquid spawn
Get in the inclined-plane solid spawn liquid medium within of above-mentioned cultivation and cultivate, make liquid spawn; Wherein composition and the weight of liquid nutrient medium (in 1000ml) are as follows: 200 grams, potato, and 20 grams of glucose, 3 grams of peptones, 1 gram of yeast extract paste, 0.5 gram, sal epsom, 1 gram of potassium primary phosphate, natural pH value, at 24 ℃, incubation time is 38 hours.
C. submerged fermentation enlarged culturing
By liquid spawn under aseptic condition successively access fill in one-level through the nutrient solution of sterilising treatment, secondary, three grades of submerged fermentation incubators, through cultivating, obtain live body bacterium liquid.
Wherein fermention medium (substratum of one-level, secondary, three grades of submerged fermentations is all identical) composition and weight are: millet 25g, glucose 15g, white sugar 5g, potassium dihydrogen sulfate 0.5g, sal epsom 0.25g, peptone 5g, yeast extract 1g, amino acid 0.1g, vitamin V B10.01g, adds water to 1000ml.
Its process following (condition of one-level, secondary, three grades of submerged fermentations is all identical): with 10% inoculum size access liquid spawn, at 25 ℃, in ventilating ratio 1:0.5 and cultivation, press under the condition for 0.05MPa, continuously ferment 38 hours, now PH is 3.9, mycelium natural subsidence 100%.
D. active somatic cells broken wall
Live body bacterium liquid is ground to (3T/H) through colloidal mill, then use high pressure homogenizer (3T/H, processing pressure 40MPa) fracturing cell walls, form suspendible glue, at 37 ℃, insulation is placed 2 hours, and intracellular matter is fully oozed out, and obtains Cordyceps mycelium fermenation raw liquid.
Step 2. prepare extracts of Chinese herbal medicine
Take the Chinese medicine material of following weight: the tuber of dwarf lilyturf 35g, radix polygonati officinalis 45g, Fructus Corni 40g, Herba Cistanches 35g, Herba Epimedii 20g, the water extraction secondary by above-mentioned Chinese medicine material with 10 times of amounts, filters to obtain extracts of Chinese herbal medicine, extracting is for the first time to extract 120 minutes at 100 ℃ of temperature, and extracting is for the second time at 100 ℃, to extract 60 minutes.
Step 3: obtain finished product after blending the former base white wine of the pure grain brew of 60 degree
By the above-mentioned Cordyceps mycelium fermenation raw liquid making, extracts of Chinese herbal medicine and the former base white wine of the 60 pure grain brew of degree, according to mass ratio, be the canned acquisition suspendible of homogeneous body finished product after 0.8:0.05:1.6 merges.
Embodiment 2
The present embodiment provides a kind of aweto health-care wine, is to adopt Cordyceps mycelium fermenation raw liquid, extracts of Chinese herbal medicine and the former base white wine of the 60 pure grain brew of degree formulated.
The preparation method of described aweto health-care wine, comprises the steps:
Step 1. prepare Cordyceps mycelium fermenation raw liquid
A. the cultivation of Cordyceps sinensis slant strains
Get natural cordyceps and carry out separate tissue, on slant medium, cultivate, make slant strains; The substratum that slant culture adopts is PDA substratum, cultivates 7 days at 25 ℃.
B. the preparation of liquid spawn
Get in the inclined-plane solid spawn liquid medium within of above-mentioned cultivation and cultivate, make liquid spawn; Wherein composition and the weight of liquid nutrient medium (in 1000ml) are as follows: 220 grams, potato, and 18 grams of glucose, 2 grams of peptones, 1.5 grams of yeast extract pastes, 0.6 gram, sal epsom, 0.5 gram of potassium primary phosphate, natural pH value, at 25 ℃, incubation time is 42 hours.
C. submerged fermentation enlarged culturing
By liquid spawn under aseptic condition successively access fill in one-level through the nutrient solution of sterilising treatment, secondary, three grades of submerged fermentation incubators, through cultivating, obtain live body bacterium liquid.
Wherein fermention medium (substratum of one-level, secondary, three grades of submerged fermentations is all identical) composition and weight are: millet 20g, glucose 15g, white sugar 5g, potassium dihydrogen sulfate 0.5g, sal epsom 0.25g, peptone 7g, yeast extract 1.5g, amino acid 0.15g, vitamin V B1 0.01g, adds water to 1000ml.
Its process following (condition of one-level, secondary, three grades of submerged fermentations is all identical): with 10% inoculum size access liquid spawn, at 23 ℃, in ventilating ratio 1:0.7 and cultivation, press under the condition for 0.05MPa, continuously ferment 36 hours, now PH is 3.8, mycelium natural subsidence 100%.
D. active somatic cells broken wall
Live body bacterium liquid is ground to (3T/H) through colloidal mill, then use high pressure homogenizer (3T/H, processing pressure 40MPa) fracturing cell walls, form suspendible glue, at 35 ℃, insulation is placed 1.5 hours, and intracellular matter is fully oozed out, and obtains Cordyceps mycelium fermenation raw liquid.
Step 2. prepare extracts of Chinese herbal medicine
Take the Chinese medicine material of following weight: the tuber of dwarf lilyturf 40g, radix polygonati officinalis 38g, Fructus Corni 40g, Herba Cistanches 35g, Herba Epimedii 19g, the water extraction secondary by above-mentioned Chinese medicine material with 10 times of amounts, filters to obtain extracts of Chinese herbal medicine, extracting is for the first time to extract 120 minutes at 100 ℃ of temperature, and extracting is for the second time at 100 ℃, to extract 60 minutes.
Step 3: obtain finished product after blending the former base white wine of the pure grain brew of 60 degree
By the above-mentioned Cordyceps mycelium fermenation raw liquid making, extracts of Chinese herbal medicine and the former base white wine of the 60 pure grain brew of degree, according to mass ratio, be the canned acquisition suspendible of homogeneous body finished product after 1:0.05:1.8 merges.
Embodiment 3
The present embodiment provides a kind of aweto health-care wine, is to adopt Cordyceps mycelium fermenation raw liquid, extracts of Chinese herbal medicine and the former base white wine of the 60 pure grain brew of degree formulated.
The preparation method of described aweto health-care wine, comprises the steps:
Step 1. prepare Cordyceps mycelium fermenation raw liquid
A. the cultivation of Cordyceps sinensis slant strains
Get natural cordyceps and carry out separate tissue, on slant medium, cultivate, make slant strains; The substratum that slant culture adopts is PDA substratum, cultivates 6 days at 23 ℃.
B. the preparation of liquid spawn
Get in the inclined-plane solid spawn liquid medium within of above-mentioned cultivation and cultivate, make liquid spawn; Wherein composition and the weight of liquid nutrient medium (in 1000ml) are as follows: 180 grams, potato, and 18 grams of glucose, 3 grams of peptones, 1 gram of yeast extract paste, 0.5 gram, sal epsom, 1.5 grams of potassium primary phosphates, natural pH value, at 24 ℃, incubation time is 36 hours.
C. submerged fermentation enlarged culturing
By liquid spawn under aseptic condition successively access fill in one-level through the nutrient solution of sterilising treatment, secondary, three grades of submerged fermentation incubators, through cultivating, obtain live body bacterium liquid.
Wherein fermention medium (substratum of one-level, secondary, three grades of submerged fermentations is all identical) composition and weight are: millet 22g, glucose 12g, white sugar 4g, potassium dihydrogen sulfate 1g, sal epsom 0.2g, peptone 7g, yeast extract 1g, amino acid 0.2g, vitamin V B1 0.01g, adds water to 1000ml.
Its process following (condition of one-level, secondary, three grades of submerged fermentations is all identical): with 10% inoculum size access liquid spawn, at 25 ℃, in ventilating ratio 1:0.6 and cultivation, press under the condition for 0.05MPa, continuously ferment 38 hours, now PH is 4.0, mycelium natural subsidence 100%.
D. active somatic cells broken wall
Live body bacterium liquid is ground to (3T/H) through colloidal mill, then use high pressure homogenizer (3T/H, processing pressure 40MPa) fracturing cell walls, form suspendible glue, at 40 ℃, insulation is placed 1.5 hours, and intracellular matter is fully oozed out, and obtains Cordyceps mycelium fermenation raw liquid.
Step 2. prepare extracts of Chinese herbal medicine
Take the Chinese medicine material of following weight: the tuber of dwarf lilyturf 42g, radix polygonati officinalis 30g, Fructus Corni 40g, Herba Cistanches 30g, Herba Epimedii 24g, the water extraction secondary by above-mentioned Chinese medicine material with 10 times of amounts, filters to obtain extracts of Chinese herbal medicine, extracting is for the first time to extract 120 minutes at 100 ℃ of temperature, and extracting is for the second time at 100 ℃, to extract 60 minutes.
Step 3: obtain finished product after blending the former base white wine of the pure grain brew of 60 degree
By the above-mentioned Cordyceps mycelium fermenation raw liquid making, extracts of Chinese herbal medicine and the former base white wine of the 60 pure grain brew of degree, according to mass ratio, be the canned acquisition suspendible of homogeneous body finished product after 0.8:0.05:1.6 merges.
Embodiment 4
The present embodiment provides a kind of aweto health-care wine, is to adopt Cordyceps mycelium fermenation raw liquid, extracts of Chinese herbal medicine and the former base white wine of the 60 pure grain brew of degree formulated.
The preparation method of described aweto health-care wine, comprises the steps:
Step 1. prepare Cordyceps mycelium fermenation raw liquid
A. the cultivation of Cordyceps sinensis slant strains
Get natural cordyceps and carry out separate tissue, on slant medium, cultivate, make slant strains; The substratum that slant culture adopts is PDA substratum, cultivates 7 days at 25 ℃.
B. the preparation of liquid spawn
Get in the inclined-plane solid spawn liquid medium within of above-mentioned cultivation and cultivate, make liquid spawn; Wherein composition and the weight of liquid nutrient medium (in 1000ml) are as follows: 200 grams, potato, and 22 grams of glucose, 4 grams of peptones, 0.5 gram of yeast extract paste, 0.6 gram, sal epsom, 1.2 grams of potassium primary phosphates, natural pH value, at 23 ℃, incubation time is 40 hours.
C. submerged fermentation enlarged culturing
By liquid spawn under aseptic condition successively access fill in one-level through the nutrient solution of sterilising treatment, secondary, three grades of submerged fermentation incubators, through cultivating, obtain live body bacterium liquid.
Wherein fermention medium (substratum of one-level, secondary, three grades of submerged fermentations is all identical) composition and weight are: millet 24g, glucose 12g, white sugar 4g, potassium dihydrogen sulfate 1.2g, sal epsom 0.1g, peptone 6g, yeast extract 2g, amino acid 0.2g, vitamin V B10.01g, adds water to 1000ml.
Its process following (condition of one-level, secondary, three grades of submerged fermentations is all identical): with 10% inoculum size access liquid spawn, at 23 ℃, in ventilating ratio 1:0.5 and cultivation, press under the condition for 0.05MPa, continuously ferment 36 hours, now PH is 3.9, mycelium natural subsidence 100%.
D. active somatic cells broken wall
Live body bacterium liquid is ground to (3T/H) through colloidal mill, then use high pressure homogenizer (3T/H, processing pressure 40MPa) fracturing cell walls, form suspendible glue, at 39 ℃, insulation is placed 1.5 hours, and intracellular matter is fully oozed out, and obtains Cordyceps mycelium fermenation raw liquid.
Step 2. prepare extracts of Chinese herbal medicine
Take the Chinese medicine material of following weight: the tuber of dwarf lilyturf 42g, radix polygonati officinalis 30g, Fructus Corni 40g, Herba Cistanches 40g, Herba Epimedii 18g, the water extraction secondary by above-mentioned Chinese medicine material with 10 times of amounts, filters to obtain extracts of Chinese herbal medicine, extracting is for the first time to extract 120 minutes at 100 ℃ of temperature, and extracting is for the second time at 100 ℃, to extract 60 minutes.
Step 3: obtain finished product after blending the former base white wine of the pure grain brew of 60 degree
By the above-mentioned Cordyceps mycelium fermenation raw liquid making, extracts of Chinese herbal medicine and the former base white wine of the 60 pure grain brew of degree, according to mass ratio, be the canned acquisition suspendible of homogeneous body finished product after 0.9:0.06:1.6 merges.
Embodiment 5
Other condition of the present embodiment is identical with embodiment 4, and its difference is in step 3 it is that Cordyceps mycelium fermenation raw liquid, extracts of Chinese herbal medicine and the former base white wine of the 60 pure grain brew of degree that employing makes is the canned acquisition suspendible of homogeneous body finished product after 0.85:0.065:1.65 merges according to mass ratio.
The content of its effective constituent cordycepin of aweto health-care wine of the present invention and adenosine meets state-promulgated pharmacopoeia completely about the quality standard requirement of Cordyceps sinensis.The aweto health-care wine of working method gained of the present invention after testing, is no less than 0.08mg containing adenosine in every 100ml, containing cordycepin, is no less than 0.42mg.This product is because being rich in amino acid, polysaccharide, adenosine and various active composition, and to diabetic subject, three high patient immunocompromised persons, sleep every day first 2 liang (also can a bu several times of drinking), take about 10 days continuously, can obviously improve symptom.
Claims (2)
1. an aweto health-care wine, it is to adopt Cordyceps mycelium fermenation raw liquid, extracts of Chinese herbal medicine and the former base white wine of the 60 pure grain brew of degree formulated, the preparation method of wherein said aweto health-care wine comprises the steps:
Step 1, prepare Cordyceps mycelium fermenation raw liquid
A. the cultivation of Cordyceps sinensis slant strains
Get natural cordyceps and carry out separate tissue, on slant medium, cultivate, make slant strains; The substratum that slant culture adopts is PDA substratum, cultivates 7 days at 25 ℃;
B. the preparation of liquid spawn
Get in the inclined-plane solid spawn liquid medium within of above-mentioned cultivation and cultivate, make liquid spawn; Wherein the composition of liquid nutrient medium and weight are as follows: 220 grams, potato, 18 grams of glucose, 2 grams of peptones, 1.5 grams of yeast extract pastes, 0.6 gram, sal epsom, 0.5 gram of potassium primary phosphate, nature pH value, at 25 ℃, incubation time is 42 hours, and wherein said liquid nutrient medium is in 1000ml;
C. submerged fermentation enlarged culturing
By liquid spawn under aseptic condition successively access fill in one-level through the nutrient solution of sterilising treatment, secondary, three grades of submerged fermentation incubators, through cultivating, obtain live body bacterium liquid;
Wherein corresponding one-level, secondary, three grades of submerged fermentation culture mediums are all identical, and its composition and weight are: millet 20g, glucose 15g, white sugar 5g, potassium dihydrogen sulfate 0.5g, sal epsom 0.25g, peptone 7g, yeast extract 1.5g, amino acid 0.15g, vitamin V B10.01g, adds water to 1000ml
Its process is as follows: with 10% inoculum size access liquid spawn, at 23 ℃, in ventilating ratio 1:0.7 and cultivation, press under the condition for 0.05MPa, continuously ferment 36 hours, now pH is 3.8, mycelium natural subsidence 100%, and in aforementioned process, the condition of one-level, secondary, three grades of submerged fermentations is all identical;
D. active somatic cells broken wall
Live body bacterium liquid is ground through 3T/H colloidal mill, then use 3T/H, the high pressure homogenizer fracturing cell walls that processing pressure is 40MPa, form suspendible glue, at 35 ℃, insulation is placed 1.5 hours, and intracellular matter is fully oozed out, and obtains Cordyceps mycelium fermenation raw liquid;
Step 2, prepare extracts of Chinese herbal medicine
Take the Chinese medicine material of following weight: the tuber of dwarf lilyturf 40g, radix polygonati officinalis 38g, Fructus Corni 40g, Herba Cistanches 35g, Herba Epimedii 19g, the water extraction secondary by above-mentioned Chinese medicine material with 10 times of amounts, filters to obtain extracts of Chinese herbal medicine, extracting is for the first time to extract 120 minutes at 100 ℃ of temperature, and extracting is for the second time at 100 ℃, to extract 60 minutes;
Step 3, obtain finished product after blending the former base white wine of the pure grain brew of 60 degree
By the above-mentioned Cordyceps mycelium fermenation raw liquid making, extracts of Chinese herbal medicine and the former base white wine of the 60 pure grain brew of degree, according to mass ratio, be the canned acquisition suspendible of homogeneous body finished product after 1:0.05:1.8 merges.
2. the preparation method of aweto health-care wine claimed in claim 1, comprises the steps:
Step 1, prepare Cordyceps mycelium fermenation raw liquid
A. the cultivation of Cordyceps sinensis slant strains
Get natural cordyceps and carry out separate tissue, on slant medium, cultivate, make slant strains; The substratum that slant culture adopts is PDA substratum, cultivates 7 days at 25 ℃;
B. the preparation of liquid spawn
Get in the inclined-plane solid spawn liquid medium within of above-mentioned cultivation and cultivate, make liquid spawn; Wherein the composition of liquid nutrient medium and weight are as follows: 220 grams, potato, 18 grams of glucose, 2 grams of peptones, 1.5 grams of yeast extract pastes, 0.6 gram, sal epsom, 0.5 gram of potassium primary phosphate, nature pH value, at 25 ℃, incubation time is 42 hours, and wherein said liquid nutrient medium is in 1000ml;
C. submerged fermentation enlarged culturing
By liquid spawn under aseptic condition successively access fill in one-level through the nutrient solution of sterilising treatment, secondary, three grades of submerged fermentation incubators, through cultivating, obtain live body bacterium liquid;
Wherein corresponding one-level, secondary, three grades of submerged fermentation culture mediums are all identical, and its composition and weight are: millet 20g, glucose 15g, white sugar 5g, potassium dihydrogen sulfate 0.5g, sal epsom 0.25g, peptone 7g, yeast extract 1.5g, amino acid 0.15g, vitamin V B10.01g, adds water to 1000ml
Its process is as follows: with 10% inoculum size access liquid spawn, at 23 ℃, in ventilating ratio 1:0.7 and cultivation, press under the condition for 0.05MPa, continuously ferment 36 hours, now pH is 3.8, mycelium natural subsidence 100%, and in aforementioned process, the condition of one-level, secondary, three grades of submerged fermentations is all identical;
D. active somatic cells broken wall
Live body bacterium liquid is ground through 3T/H colloidal mill, then use 3T/H, the high pressure homogenizer fracturing cell walls that processing pressure is 40MPa, form suspendible glue, at 35 ℃, insulation is placed 1.5 hours, and intracellular matter is fully oozed out, and obtains Cordyceps mycelium fermenation raw liquid;
Step 2, prepare extracts of Chinese herbal medicine
Take the Chinese medicine material of following weight: the tuber of dwarf lilyturf 40g, radix polygonati officinalis 38g, Fructus Corni 40g, Herba Cistanches 35g, Herba Epimedii 19g, the water extraction secondary by above-mentioned Chinese medicine material with 10 times of amounts, filters to obtain extracts of Chinese herbal medicine, extracting is for the first time to extract 120 minutes at 100 ℃ of temperature, and extracting is for the second time at 100 ℃, to extract 60 minutes;
Step 3, obtain finished product after blending the former base white wine of the pure grain brew of 60 degree
By the above-mentioned Cordyceps mycelium fermenation raw liquid making, extracts of Chinese herbal medicine and the former base white wine of the 60 pure grain brew of degree, according to mass ratio, be the canned acquisition suspendible of homogeneous body finished product after 1:0.05:1.8 merges.
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| CN104357282A (en) * | 2014-11-21 | 2015-02-18 | 西宁意格知识产权咨询服务有限公司 | Preparation method of fermented health liquor with cordyceps sinensis and nitrariatangutorum |
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| CN103160421B (en) * | 2013-04-17 | 2014-08-27 | 香格里拉县宜康宝生物科技有限公司 | Method for preparing cordyceps sinensis wine |
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| CN1320101C (en) * | 2005-09-19 | 2007-06-06 | 王永年 | Aweto cytosol preparing process |
| CN101612305B (en) * | 2009-07-13 | 2012-05-30 | 尚兰根 | Aweto health-care wine and processing method thereof |
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