CN1320101C - Aweto cytosol preparing process - Google Patents
Aweto cytosol preparing process Download PDFInfo
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- CN1320101C CN1320101C CNB2005100128330A CN200510012833A CN1320101C CN 1320101 C CN1320101 C CN 1320101C CN B2005100128330 A CNB2005100128330 A CN B2005100128330A CN 200510012833 A CN200510012833 A CN 200510012833A CN 1320101 C CN1320101 C CN 1320101C
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Abstract
The present invention discloses a method for preparing Chinese caterpillar fungus cell sap. The method comprises the following steps: (1) Chinese caterpillar fungus slant strains are cultured, and a culture medium used by the culture contains 0.1% to 1% of dried silkworm chrysalis meal represented according to mass percentage; (2) liquid strains are prepared; (3) submerged fermentation enlarging culture is carried out, three stages of submerged fermentation culture solution all contain the water extract of dried silkworm chrysalis meal; (4) living body bacterium liquid cell wall is broken; after the pH value of the living body bacterium liquid is adjusted to 6.1 to 6.7, the living body bacterium liquid is heated to 40 DEG C to 50 DEG C, and the temperature is maintained for 15 to 5 hours, and cell sap raw liquor is obtained; (5) after the cell sap raw liquor is filtered, degassed, bottled and sterilized, a Chinese caterpillar fungus cell sap finished product is obtained. The preparation method of the present invention has the advantage of simple technology, the effective components of the prepared Chinese caterpillar fungus wall breaking cell sap are close to those of natural Chinese caterpillar fungus, and the prepared Chinese caterpillar fungus wall breaking cell sap has rich nutrition, is easily absorbed by a human body, and is safe to drink. The present invention is easy to implement in industrialization production.
Description
Technical field
The present invention relates to the preparation method of Cordyceps sinensis enchylema, belong to technical field of bioengineering.
Background technology
Cordyceps sinensis is a kind of rare rare Chinese medicine, and numerous disease is had the treatment effect, can improve human body immunologic function and delay senility, obtain the especially attention of domestic and international medical treatment, food circle.At present, research and development medicinal to Chinese caterpillar fungus and health-care products both at home and abroad is just in the ascendant, particularly domestic, and research and development in this respect are like a raging fire especially, with Cordyceps sporophore and mycelium thereof is existing tens kinds of the medicinal preparations of raw material production and health-care preparation, and in continuous release.These medicinal preparationss are in treatment cardiovascular disorder, hepatic diseases, kidney disease, respiratory system disease, hemopathy and as the aspects such as adjuvant drug of antineoplaston all having obtained gratifying curative effect.These Products Development have further been widened the clinical application range of Chinese caterpillar fungus preparation, have expanded the Application Areas of Chinese caterpillar fungus.The most representative in the above-mentioned preparation is Chinese caterpillar fungus oral liquid.But disclosed Chinese caterpillar fungus oral liquid has the following disadvantages in the prior art: at first be that its culture medium prescription is unreasonable.Because Cordyceps sinensis is to be the host of Cordyceps with bat moth two instars, and two instars are complicated organism aggregates, its contained nutritive ingredient is very complicated, comprehensive, so far there is not definite composition report, therefore, substratum with present routine is cultivated Cordyceps sinensis, can't reach Cordyceps sinensis desired nutritional composition; Secondly, public use to will mycetocyte and culture residue binding substances being precipitated in the lump with chemical reagent in the treatment process of fermented liquid in the prior art, destroyed the due composition of Cordyceps sinensis and made it change character, nature can bring incompatibility and insecurity to the user, but also has lost the natural sex of Cordyceps sinensis matter; Moreover the Chinese caterpillar fungus oral liquid of prior art for preparing is unfavorable for absorption of human body, and especially concerning old or patient, curative effect and nourishing function are not obvious.The existence of above-mentioned defective has influenced the normal performance that Cordyceps sinensis is medicinal and health care is worth, and having restricted is the technological development and the industry development of main raw material with Cordyceps sinensis.
Summary of the invention
The preparation method who the purpose of this invention is to provide a kind of Cordyceps sinensis enchylema, its product is similar to the Cordyceps sinensis natural sex, solve the security of effective constituent and the stability problem of content, and improved drug effect, the health-care effect of Cordyceps fungus and the security of taking.
Design of the present invention is such.In order to guarantee the nutrition of Cordyceps sinensis enchylema, at first to satisfy the culture condition of Cordyceps, particularly the nutrition of substratum should be close with the contained nutrition of its host bat moth two instars, and the bacterial classification that so just can make cultivation is near natural Cordyceps sinensis composition.Through repeated detection and test, the present invention determines to select dried silkworm chrysalis meal or dried silkworm chrysalis meal water extract as additive, in order to the nutritive ingredient of rich medium matter, and is added on respectively in solid spawn and the liquid spawn culture medium.
In addition, in order to improve the assimilation effect of product, the present invention has abandoned micronizing idea in the past makes Cordyceps sinensis enchylema become broken wall enchylema, because ultramicrotechnique is applicable to solid dielectric, and be not suitable for the cracking of live body bacterium liquid cell walls, particularly micronizing produces certain temperature and will destroy the effective constituent of machining object to a certain extent.Given this, the biological enzyme that the present invention utilizes live body bacterium liquid self to produce makes its cell wall breaking, specific descriptions are that live body bacterium liquid is under the certain temperature condition, substratum in the live body bacterium liquid produces the glucosan enzyme and these two kinds of biological enzymes of chitinase then connect cell key-hydrogen bond blocking-up, cell walls is cracked, obtain broken wall enchylema thus.
Feature of the present invention also is the processing of fermented liquid is not used as chemical reagent such as zinc acetates, to guarantee that special Chemical Composition in the Cordyceps such as cordycepin, D-N.F,USP MANNITOL, Cordyceps polysaccharide, adenosine, uridine, SOD etc. are not damaged and modification, its drug effect and security have been guaranteed.
Particularly, method of the present invention comprises the steps:
(1) cultivation of Cordyceps sinensis slant strains
Get natural cordyceps and carry out separate tissue, on slant medium, cultivate, make slant strains;
The dried silkworm chrysalis meal that contains the 0.1-1% that represents by mass percent in the used substratum;
Culture condition is: temperature 20-26 ℃, and incubation time 15-7 days;
(2) preparation of liquid spawn
Get solid spawn fill in the container of the nutrient solution of sterilising treatment, cultivate 7 days after, make liquid spawn;
Contain dried silkworm chrysalis meal water extract in the used nutrient solution;
(3) submerged fermentation enlarged culturing
Liquid spawn inserting successively under the aseptic condition in the one-level fill through the nutrient solution of sterilising treatment, secondary, the three grades of submerged fermentation incubators, is obtained live body bacterium liquid through cultivation;
Three grades of submerged fermentation liquid spawn access amounts are respectively in 10%, three grade of submerged fermentation nutrient solution cultivating liquid measure and all contain dried silkworm chrysalis meal water extract;
(4) live body bacterium liquid cell wall breaking
After live body bacterium liquid pH value transferred to 6.1-6.7, be warming up to 40-50 ℃, kept 15-5 hour, obtain enchylema stoste;
(5) filter, outgas, bottle, sterilize
Above-mentioned enchylema stoste through filter just filter, after the smart filter, the vacuum decompression degassing, bottling capping, sterilization broken wall enchylema finished product.
Used strain cultivation liquid is such in step (2) and step (3): contain the water extract that useful 1-10 gram dried silkworm chrysalis meal makes in the per kilogram nutrient solution.
The effect that the present invention obtains is: the mycelium that utilizes method of the present invention to turn out, 8.3 times of demarcating content above state-promulgated pharmacopoeia; Product Cordyceps sinensis enchylema stoste surpasses 2.7～3.9 times of state-promulgated pharmacopoeia demarcation component content; Cordyceps sinensis enchylema both can dilute as required, also can concentrate as required, and can add other liquor as required; Present method can also conveniently be extracted wherein a certain special ingredient or comprehensively use its herb ingredient, processes very convenient.
It is pointed out that cell wall breaking Technology of the present invention is equally applicable to as the cell wall breaking of the live body bacterium liquid of all rare fungies such as hedgehog hydnum, glossy ganoderma and makes its enchylema.
Embodiment
Following examples are in order to explanation the present invention.
Embodiment 1
(1) cultivation of Cordyceps sinensis slant strains
The substratum moiety is: glucose 2%, peptone 0.1%, dried silkworm chrysalis meal 0.1%, potassium primary phosphate 0.1%, sal epsom 0.05%, agar 2%, and all the other are sterilized water (tap water of available sterilization);
Culture condition is: 20 ℃ of temperature, incubation time 15 days;
Get natural cordyceps and carry out separate tissue, on substratum, cultivate, make slant strains;
(2) preparation of liquid spawn
By 1 kilogram of nutrient solution total mass, it consists of: the water extract of glucose 2%, peptone 0.1%, potassium primary phosphate 0.1%, sal epsom 0.05%, 1 gram dried silkworm chrysalis meal, and all the other are sterilized water (tap water of available sterilization);
Get 30 minutes postcooling to 25 of about 1/4, the 121 ℃ of sterilization of above-mentioned substratum dress triangular flask ℃, with solid spawn 1cm
2Some are inserted under aseptic condition, then become bacteria suspension and show that the mycelia fragment is full of fermentation with a large amount of mycelium pellets and becomes clear liquid through about 7 days, are liquid spawn:
(3) submerged fermentation enlarged culturing
Liquid spawn is inserted under aseptic condition in the one-level submerged fermentation incubator, and the access amount is 10% of a nutrient solution, and nutrient solution inserts under aseptic condition after sterilizing.Secondary then, three grades of submerged fermentations cultivate that the rest may be inferred, end to carry out next program when nutrient solution becomes clear and has a large amount of mycelia fragments and mycelium pellet to occur again;
(4) live body bacterium liquid cell wall breaking
After live body bacterium liquid pH value transferred to 6.1, be warming up to 40 ℃, kept 15 hours, obtain broken wall enchylema stoste;
(5) filter, outgas, bottle, sterilize
Above-mentioned enchylema stoste is just filtered, smart filter, the vacuum decompression degassing, taken off charcoal and handle through filter, and the sterilization of can bottling obtains the Chinese caterpillar fungus oral liquid stoste that can directly take.
This stoste can be added jujube juice or Sucus Ziziphi Spinosae etc. and be regulated mouthfeel.
Embodiment 2
(1) cultivation of Cordyceps sinensis slant strains
Medium component: glucose 2%, peptone 0.1%, dried silkworm chrysalis meal 0.1%, potassium primary phosphate 0.1%, sal epsom 0.05%, agar 2%, all the other are sterilized water (available tap water through sterilization);
Culture condition is: 24 ℃ of temperature, incubation time 10 days; Get natural cordyceps and carry out separate tissue, on substratum, cultivate, make slant strains;
(2) preparation of liquid spawn
By 1 kilogram of nutrient solution total mass, consist of: glucose 2%, peptone 0.1%, potassium primary phosphate 0.1%, sal epsom 0.05%, 5 gram dried silkworm chrysalis meal water extracts, all the other are sterilized water (tap water of available sterilization);
Get 30 minutes postcooling to 25 of about 1/4, the 121 ℃ of sterilization of above-mentioned substratum dress triangular flask ℃, with solid spawn 1cm
2Some are inserted under aseptic condition, then become bacteria suspension and show that the mycelia fragment is full of fermentation with a large amount of mycelium pellets and becomes clear liquid through about 7 days, are liquid spawn:
(3) submerged fermentation enlarged culturing
Liquid spawn is inserted under aseptic condition in the one-level submerged fermentation incubator, and the access amount is 10% of a nutrient solution, and nutrient solution inserts under aseptic condition after sterilizing.Secondary then, three grades of submerged fermentations cultivate that the rest may be inferred, end to carry out next program when nutrient solution becomes clear and has a large amount of mycelia fragments and mycelium pellet to occur again;
(4) live body bacterium liquid cell wall breaking
After live body bacterium liquid pH value transferred to 6.4, be warming up to 45 ℃, kept 10 hours, obtain broken wall enchylema stoste;
(5) filter, outgas, bottle, sterilize
Above-mentioned enchylema stoste is just filtered, smart filter, the vacuum decompression degassing, taken off charcoal and handle through filter, and the sterilization of can bottling obtains the Chinese caterpillar fungus oral liquid stoste that can directly take.
This stoste can be added jujube juice or Sucus Ziziphi Spinosae etc. and be regulated mouthfeel.
Embodiment 3
(1) cultivation of Cordyceps sinensis slant strains
Medium component is: glucose 2%, peptone 0.1%, dried silkworm chrysalis meal 0.1%, potassium primary phosphate 0.1%, sal epsom 0.05%, agar 2%, and all the other are sterilized water (tap water of available sterilization);
Culture condition is: 26 ℃ of temperature, incubation time 7 days;
Get natural cordyceps and carry out separate tissue, on substratum, cultivate, make slant strains;
(2) preparation of liquid spawn
By 1 kilogram of nutrient solution total mass, form and divide: glucose 2%, peptone 0.1%, potassium primary phosphate 0.1%, sal epsom 0.05%, 10 gram dried silkworm chrysalis meal water extracts, all the other are sterilized water (tap water of available sterilization);
Get 25 ℃ of 30 minutes postcooling systems of about 1/4, the 121 ℃ of sterilization of above-mentioned substratum dress triangular flask, with solid spawn 1cm
2Some are inserted under aseptic condition, then become bacteria suspension and show that the mycelia fragment is full of fermentation with a large amount of mycelium pellets and becomes clear liquid through about 7 days, are liquid spawn:
(3) submerged fermentation enlarged culturing
Liquid spawn is inserted under aseptic condition in the one-level submerged fermentation incubator, and the access amount is 10% of a nutrient solution, and nutrient solution inserts under aseptic condition after sterilizing.Secondary then, three grades of submerged fermentations cultivate that the rest may be inferred, end to carry out next program when nutrient solution becomes clear and has a large amount of mycelia fragments and mycelium pellet to occur again;
(4) live body bacterium liquid cell wall breaking
After live body bacterium liquid pH value transferred to 6.7, be warming up to 50 ℃, kept 5 hours, obtain broken wall enchylema stoste;
(5) filter, outgas, bottle, sterilize
Above-mentioned enchylema stoste is just filtered, smart filter, the vacuum decompression degassing, taken off charcoal and handle through filter, and the sterilization of can bottling obtains the Chinese caterpillar fungus oral liquid stoste that can directly take.
This stoste can be added jujube juice or Sucus Ziziphi Spinosae etc. and be regulated mouthfeel.
Claims (2)
1, the preparation method of Cordyceps sinensis enchylema is characterized in that may further comprise the steps:
(1) cultivation of Cordyceps sinensis slant strains
Get natural cordyceps and carry out separate tissue, on slant medium, cultivate, make slant strains;
The dried silkworm chrysalis meal that contains the 0.1-1% that represents by mass percent in the used substratum;
Culture condition is: controlled temperature 20-26 ℃, and incubation time 15-7 days;
(2) preparation of liquid spawn
Get solid spawn and cultivated 7 days filling in the container of the nutrient solution of sterilising treatment, make liquid spawn;
Contain dried silkworm chrysalis meal water extract in the used nutrient solution;
(3) submerged fermentation enlarged culturing
Liquid spawn inserting successively under the aseptic condition in the one-level fill through the nutrient solution of sterilising treatment, secondary, the three grades of submerged fermentation incubators, is obtained live body bacterium liquid through cultivation;
Three grades of submerged fermentation liquid spawn access amounts are respectively in 10%, three grade of submerged fermentation nutrient solution cultivating liquid measure and all contain dried silkworm chrysalis meal water extract;
(4) live body bacterium liquid cell wall breaking
After live body bacterium liquid pH value transferred to 6.1-6.7, be warming up to 40-50 ℃, kept 15-5 hour, make self to produce dextranase in the live body bacterium liquid and chitinase cracks its cell walls, obtain broken wall enchylema stoste;
(5) filter, outgas, bottle, sterilize
Above-mentioned enchylema stoste through filter just filter, after the smart filter, the vacuum decompression degassing, bottling capping, sterilization the Cordyceps sinensis enchylema finished product.
2, by the preparation method of the described Cordyceps sinensis enchylema of claim 1, it is characterized in that: used strain cultivation liquid is such in step (2) and step (3): the water extract that contains useful 1-10 gram dried silkworm chrysalis meal in the per kilogram nutrient solution.
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| CNB2005100128330A CN1320101C (en) | 2005-09-19 | 2005-09-19 | Aweto cytosol preparing process |
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| CNB2005100128330A CN1320101C (en) | 2005-09-19 | 2005-09-19 | Aweto cytosol preparing process |
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| CN1320101C true CN1320101C (en) | 2007-06-06 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104357282A (en) * | 2014-11-21 | 2015-02-18 | 西宁意格知识产权咨询服务有限公司 | Preparation method of fermented health liquor with cordyceps sinensis and nitrariatangutorum |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102273379A (en) * | 2011-08-08 | 2011-12-14 | 曾树生 | Method for producing cordyceps moon cakes |
| CN102746974B (en) * | 2012-07-25 | 2014-01-22 | 陈合龙 | Cordyceps sinensis health wine and preparation method thereof |
| CN103169065A (en) * | 2013-03-21 | 2013-06-26 | 武汉蜀泰科技有限公司 | Wall breaking method of cordyceps sinensis |
| CN103271951B (en) * | 2013-05-23 | 2015-04-01 | 台建祥 | Method for preparing cordyceps preparations with high adenosine contents |
| CN112795493B (en) * | 2021-04-02 | 2023-07-07 | 青海珠峰冬虫夏草原料有限公司 | Method for improving yield of fermented cordyceps sinensis mycelia |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1095103A (en) * | 1993-05-07 | 1994-11-16 | 中国中医研究院 | Producing process of Chinese caterpillar fungus hypha fermentation |
| CN1104249A (en) * | 1994-08-25 | 1995-06-28 | 俞永信 | Deep fermentation technology for cordyceps sinensis sacc |
| CN1640488A (en) * | 2004-01-09 | 2005-07-20 | 蒋朝宗 | Method for preparing supermicro powder preparation of cordyceps sinensis mycelium |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1095103A (en) * | 1993-05-07 | 1994-11-16 | 中国中医研究院 | Producing process of Chinese caterpillar fungus hypha fermentation |
| CN1104249A (en) * | 1994-08-25 | 1995-06-28 | 俞永信 | Deep fermentation technology for cordyceps sinensis sacc |
| CN1640488A (en) * | 2004-01-09 | 2005-07-20 | 蒋朝宗 | Method for preparing supermicro powder preparation of cordyceps sinensis mycelium |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104357282A (en) * | 2014-11-21 | 2015-02-18 | 西宁意格知识产权咨询服务有限公司 | Preparation method of fermented health liquor with cordyceps sinensis and nitrariatangutorum |
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