CN1104249A - Deep fermentation technology for cordyceps sinensis sacc - Google Patents

Deep fermentation technology for cordyceps sinensis sacc Download PDF

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CN1104249A
CN1104249A CN 94115054 CN94115054A CN1104249A CN 1104249 A CN1104249 A CN 1104249A CN 94115054 CN94115054 CN 94115054 CN 94115054 A CN94115054 A CN 94115054A CN 1104249 A CN1104249 A CN 1104249A
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俞永信
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Zhang Xuefeng
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Abstract

The present invention provides a commercial culture propagation process of Chinese pilose spore of asexual stage of Chinese caterpillar fungus, and the cultured product mycelia of Chinese caterpillar fungus have the pharmacological action identical to that of wild Chinese caterpillar fungus, and the necessary amino acid content of artificial-cultured Chinese caterpillar fungus is more than that of natural Chinese caterpillar fungus by times.

Description

Deep fermentation technology for cordyceps sinensis sacc
The present invention be Cordyceps sinensis the imperfect stage-(on November 3rd, 1987 was preserved in Chinese typical culture collection center, and preserving number is: deep layer fermenting process technology CCTCC NO:M87101), batch production are produced winter worm summer herb thallus by a hair spore in China.
Cordyceps sinensis is the health-care tonic treasure that only originates in the Southwest China northwest plateau.Wild Cordyceps sinensis, the producing region limitation, the output rareness, resource shortage costs an arm and a leg.China is by the hair spore and finish the technology of perfect stage Cordyceps sinensis, with China by mao spore under given conditions, infects the host-Hepialus oblifurcus Chu et Wang larva of artificial breeding, finishes the perfect stage, and the cycle is long, the requirement condition strictness, and cost is higher.Advantage of the present invention is: the deep layer fermenting process condition is controlled easily, cycle is shorter, cost is lower, the winter worm summer herb thallus composition of fermentation gained and natural cordyceps constituent class are together, and can induce the meta-bolites that produces the human needs, and can control mycelium production artificially, be used to the invigorant that keeps healthy, delays senility as raw material production, the medicine of being used for the treatment of property, can satisfy to greatest extent that people increase day by day to health care, diseases prevention, the needs of curing the disease.
Below be described in detail main contents of the present invention:
1, slant culture:
Slant medium: 10 milligrams of MgSO of wheat bran 30 gram peptones, 10 gram milk powder 10 gram dried silkworm chrysalis meals, 3 gram VITAMIN 47H 21000 milliliters in O0.5 gram potassium primary phosphate 1 gram agar 20 gram water.
With China by a hair spore (Hirsutella sinensis yu, Liu, Guo et Fang, sp.nov) (CCTCC NO:M87101) inoculation was cultivated under 15-18 ℃ condition 30 days on slant medium;
2, liquid culture:
Liquid nutrient medium: 10 milligrams of MgSO of wheat bran 30 gram milk powder, 10 gram dried silkworm chrysalis meal juice 3 gram beef broths, 10 gram VITAMIN 47H 21000 milliliters in O0.5 gram potassium primary phosphate 1 gram water, PH6.5, the packing triangular flask, volume is 1/5 of a triangular flask capacity, the rearmounted shaking table of sterilizing, under 18 ± 2 ℃ temperature, shaking culture is after 8 days on 90 times/minute the reciprocating type shaking table, mycelium promptly enters the growth animated period, can carry out enlarged culturing again.
3, fermentative production
(1) seed tank culture
Substratum: 1000 liters of PH6.5 of 10 kilograms of VITAMIN of 3 kilograms of yeast of 10 kilograms of dried silkworm chrysalis meal juice of 30 kilograms of milk powder of wheat bran, 10 gram water, insert the liquid culture mycelia with 5% inoculum size, 18 ± 2 ℃ of temperature, air flow 1: under condition 1(V/V/V), continuously fermented 168 hours.This moment, mycelial growth was vigorous, through the microscopy no bacteria pollution, can change fermentor tank production over to.
(2) fermentor tank production
Substratum: 10 kilograms of VITAMIN of 3 kilograms of yeast powders of 10 kilograms of dried silkworm chrysalis meal juice of 30 kilograms of milk powder of wheat bran, 10 gram MgSO 47H 21000 kilograms of O0.5 kilogram potassium primary phosphate 1 kg water.Fermentation condition: 18 ± 2 ℃ of temperature, air flow 1: 1(V/V/V), PH6.5 continuously fermented 7 days, behind the 24th hour of fermentation beginning, got the fermented liquid sample every 16 hours, measure pH value, reducing sugar and ammonia-state nitrogen, when PH drops to 5.5-5.0, reducing sugar is below 1%, ammonia-state nitrogen when the microscopy mycelium has produced a large amount of conidiophores and conidium, just can go out jar below 0.1 ‰.
Embodiment 1 cultural mycelium and Cordyceps sinensis composition are relatively
Figure 941150542_IMG1
The comparison of embodiment 2 natural cordyceps and Chinese caterpillar fungus thalline essential amino acids content
It is as follows that Zhejiang Agricultural Univ is analyzed the result with the 835-50 of Hitachi amino acidanalyser to natural cordyceps and Chinese caterpillar fungus thalline:
Figure 941150542_IMG2
* tryptophane all destroys in hydrolytic process, can't measure.
From The above results, indispensable amino acid is all at double in natural cordyceps.

Claims (3)

1, China is by hair spore (Hirsutella sinensis yu, Liu, Guo et Fang, sp.nov. preserving number: zymotechnique CCTCC NO:M87101), it is characterized in that getting fresh Cordyceps sinensis polypide (sclerotium) partly, remove epidermis, thinly slice, deposit and state culture medium culturing, or the picking thecaspore is cultivated
Slant medium: 1000 milliliters in 10 milligrams of sal epsom of wheat bran 30 gram peptones, 10 gram milk powder 10 gram dried silkworm chrysalis meals, 3 gram VITAMIN, 0.5 gram potassium primary phosphate, 1 gram agar, 20 gram water, PH6.5 cultivated 30 days under 15-18 ℃ temperature;
Liquid nutrient medium: 1000 milliliters in wheat bran 30 gram milk powder 10 gram dried silkworm chrysalis meal juice 3 gram beef broths 10 gram VITAMIN 3 gram sal epsom 0.5 gram potassium primary phosphate 1 gram water, PH 6.5, under 18 ± 2 ℃ temperature, shaking culture enlarged culturing after 8 days on 90 times/minute the reciprocating type shaking table.
2, according to claim 1, China is by hair spore zymotechnique technology, it is characterized in that the seeding tank fermention medium: 1000 milliliters of PH6.5 air flows 1 of wheat bran 30 gram milk powder, 10 gram dried silkworm chrysalis meal juice 3 gram yeast powders, 10 gram 10 milligrams of water of VITAMIN: 1(V/V) the condition bottom fermentation 18 ± 2 ℃ of temperature changes jar, inoculum size 5% after 7 days.
3, according to a claim 1 and the described China of claim 2 a quilt hair spore fermentation technique, it is characterized in that fermentation tank culture medium: 18 ± 2 ℃ of air flows 1 of 10 kilograms of VITAMIN of 3 kilograms of yeast powders of 10 kilograms of dried silkworm chrysalis meal juice of 30 kilograms of milk powder of wheat bran, 10 gram sal epsom 1000 kilograms of temperature of 0.5 kilogram of potassium primary phosphate, 1 kg water: 1(V/V) continuously fermenting went out jar in 168 hours.
CN94115054A 1994-08-25 1994-08-25 Deep fermentation technology for cordyceps sinensis sacc Expired - Lifetime CN1036531C (en)

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Cited By (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1078051C (en) * 1998-07-21 2002-01-23 李健 Method for preparing health-care food contg. chinese caterpillar fungus
CN1082089C (en) * 1996-08-16 2002-04-03 俞永信 Fermentation technology of xylaria nigripes
CN1312271C (en) * 2004-02-27 2007-04-25 俞永信 Method of whole manual culture for chinese caterpillar fungus
CN1320101C (en) * 2005-09-19 2007-06-06 王永年 Aweto cytosol preparing process
CN1323157C (en) * 2005-09-23 2007-06-27 田向荣 Chinese caterpillar fungus bionic culturing method
CN100344748C (en) * 2005-04-07 2007-10-24 雷明 Culture medium for improving fermentation yield of aweto mycelium and method for preparing same
CN100374542C (en) * 2003-08-19 2008-03-12 俞永信 Cordyceps vegetative stage continuous fermentation technology
CN102106235A (en) * 2010-12-24 2011-06-29 王梅平 Total artificial method for cultivating cordyceps sinensis
CN103005402A (en) * 2012-12-31 2013-04-03 厦门金达威集团股份有限公司 Method for producing cordyceps sinensis strain powder through liquid fermentation
CN103483037A (en) * 2013-09-05 2014-01-01 东方中科生命科学有限责任公司 Cordyceps liquid culture medium and application thereof
CN105000958A (en) * 2015-06-23 2015-10-28 中山鼎晟生物科技有限公司 Solid medium of cordyceps sinensis and method of solid fermentation
CN105505784A (en) * 2015-12-11 2016-04-20 辽宁省农业科学院食用菌研究所 Cordyceps militaris culture medium capable of improving cordycepin content, and preparation method of cordyceps militaris culture medium
WO2016082327A1 (en) * 2014-11-26 2016-06-02 南京中科药业有限公司 Processing method of cordyceps sinensis mycelium
CN107118003A (en) * 2017-05-20 2017-09-01 天津市东方中滨农业科技有限公司 A kind of breeding method of Cordyceps militaris fermented bacterium and application

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN100448456C (en) * 2006-05-22 2009-01-07 安徽农业大学 Chinese pilose spore concentrated oral liquid
CN102835245B (en) * 2011-06-24 2014-03-26 浙江泛亚生物医药股份有限公司 Bionic culture method for cordyceps sobolifera

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN85107991A (en) * 1985-11-01 1987-05-06 北京市营养源研究所 The industrial process of Cordyceps mycelium

Cited By (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1082089C (en) * 1996-08-16 2002-04-03 俞永信 Fermentation technology of xylaria nigripes
CN1078051C (en) * 1998-07-21 2002-01-23 李健 Method for preparing health-care food contg. chinese caterpillar fungus
CN100374542C (en) * 2003-08-19 2008-03-12 俞永信 Cordyceps vegetative stage continuous fermentation technology
CN1312271C (en) * 2004-02-27 2007-04-25 俞永信 Method of whole manual culture for chinese caterpillar fungus
CN100344748C (en) * 2005-04-07 2007-10-24 雷明 Culture medium for improving fermentation yield of aweto mycelium and method for preparing same
CN1320101C (en) * 2005-09-19 2007-06-06 王永年 Aweto cytosol preparing process
CN1323157C (en) * 2005-09-23 2007-06-27 田向荣 Chinese caterpillar fungus bionic culturing method
CN102106235A (en) * 2010-12-24 2011-06-29 王梅平 Total artificial method for cultivating cordyceps sinensis
CN103005402A (en) * 2012-12-31 2013-04-03 厦门金达威集团股份有限公司 Method for producing cordyceps sinensis strain powder through liquid fermentation
CN103005402B (en) * 2012-12-31 2014-07-23 厦门金达威集团股份有限公司 Method for producing cordyceps sinensis strain powder through liquid fermentation
CN103483037A (en) * 2013-09-05 2014-01-01 东方中科生命科学有限责任公司 Cordyceps liquid culture medium and application thereof
CN103483037B (en) * 2013-09-05 2015-06-24 东方中科生命科学有限责任公司 Cordyceps liquid culture medium and application thereof
WO2016082327A1 (en) * 2014-11-26 2016-06-02 南京中科药业有限公司 Processing method of cordyceps sinensis mycelium
CN105000958A (en) * 2015-06-23 2015-10-28 中山鼎晟生物科技有限公司 Solid medium of cordyceps sinensis and method of solid fermentation
CN105505784A (en) * 2015-12-11 2016-04-20 辽宁省农业科学院食用菌研究所 Cordyceps militaris culture medium capable of improving cordycepin content, and preparation method of cordyceps militaris culture medium
CN107118003A (en) * 2017-05-20 2017-09-01 天津市东方中滨农业科技有限公司 A kind of breeding method of Cordyceps militaris fermented bacterium and application

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