CN107177515A - A kind of ganoderma lucidum solid spawn and its application in ganoderma lucidum liquid submerged fermentation - Google Patents
A kind of ganoderma lucidum solid spawn and its application in ganoderma lucidum liquid submerged fermentation Download PDFInfo
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Abstract
The invention discloses a kind of ganoderma lucidum solid spawn and its application in ganoderma lucidum liquid submerged fermentation, it is that compost is made using peanut shell powder and wheatfeed or fine rice bran as raw material, ganoderma lucidum slant strains using activation is parent species, light culture is carried out in seed bottle or polypropylene plastics bacterium bag, the ganoderma lucidum solid spawn is made.With traditional liquid strain（Based on mycelium pellet）Compare, solid spawn of the present invention has that strain cost is low, long-time preservation and can be directly used for the advantage of liquid fermentation, the frequent preparation of fermented bacterium can be avoided, is conducive to improving operating efficiency, and during using it as the strain of ganoderma lucidum liquid Submerged fermentation, because its decentralization is high, a large amount of mycelial growth points can be formed, so as to which glossy ganoderma fermentation efficiency is greatly improved, significantly improve ganoderma lucidum exocellular polysaccharide and biomass, fermentation pollution rate can be also reduced, with great application prospect.
Description
Technical field
The invention belongs to medicinal fungi and application of biological engineering field, and in particular to a kind of ganoderma lucidum solid spawn and its in spirit
Application on sesame liquid deep layer fermenting.
Background technology
Ganoderma lucidum（Ganoderma lucidum）Also known as celestial grass, seocho, refreshing sesame, not old grass, it belongs to Basidiomycetes, agaric
Mesh, Polyporaceae, Ganoderma fungi, are grown on the different dead or broad leaf tree trunks in growth, on branch, ancients think it
With the magical effect come back to life.Ganoderma lucidum refers mainly to include red sesame, purple sesame and Ganoderma tsugae etc. in China has medical value
Sesame class.Ganoderma lucidum is in existing more than the 2000 years medicinal history of the oriental countries such as China, available for hepatitis, hypertension, high fat of blood and stomach
The treatment of a variety of diseases such as cancer.Ganoderma lucidum is warm-natured, sweet, slight bitter, the beneficial motive, green blood, helps heart and tranquilizing mind, beneficial lung qi, hard muscles and bones, profit
A variety of functions such as joint, tonifying liver of calming the nerves.Edible glossy ganoderma can promote longevity, strengthen physique.
Ganoderma lucidum contains abundant active component, and such as polysaccharide, triterpenes, sterol, nucleic acid, protein, alkaloid, furans derive
Thing etc., wherein polysaccharide and triterpene are the major function active components of ganoderma lucidum.GL-B have anticancer, antitumor, immunological regulation,
Anti-aging, anti-oxidant etc. are acted on；Ganodenic acid has antitumor, anti AIDS virus, antibacterial and antioxidation activity.Due to polysaccharide
And triterpene has a variety of physiologically actives, its a large amount of efficiently production turns into significant concern point.But GL-B and three so far
Terpene（Predominantly ganoderic acid）Deng the production of active components of glossy ganoderma, still to rely primarily on the fructification manually cultivated be raw material, is used
Microwave, the method for ultrasound assisted extraction are obtained, and be there is low yield, be difficult to large-scale production and the activity of different batches fructification
Composition is constituted and content has the shortcomings that larger difference, causes ganoderma lucidum product price height and product to be difficult to standardized production.
Different from traditional cultivation method, ganoderma lucidum liquid submerged fermentation has that with short production cycle, process is easy to control, production cost
It is low, occupy little space and can industrialized production the advantages of, furthermore, it has been established that Ganoderma lucidum submerged fermentation product such as GL-B and three
Terpene has the bioactivity close with fructification, therefore, deep as the liquid of target product using GL-B, triterpene and biomass
Layer fermentation turns into the focus that ganoderma lucidum is studied.At present, ganoderma lucidum liquid submerged fermentation is generally used as fermentation using bacterium ball-type liquid spawn
Seed, but Mycelium Growth of Ganoderma lucidum is slow, causes microorganism easily occurs in strain long preparation period, and operation and incubation dirty
Dye, causes strain to prepare failure, influence production, and zymotechnique requirement frequently prepares liquid spawn, and process is cumbersome.Therefore, have
Necessity exploitation is adapted to the new mushroom-seed culturing of ganoderma lucidum liquid submerged fermentation and sets up the zymotechnique adaptable with it.
The content of the invention
It is an object of the invention to provide a kind of ganoderma lucidum solid spawn and its application in ganoderma lucidum liquid submerged fermentation.
To achieve the above object, the present invention is adopted the following technical scheme that：
A kind of ganoderma lucidum solid spawn, is to be mixed in proportion after peanut shell powder is crushed with wheatfeed or fine rice bran, in terms of dry weight, mixes
Close material in weight shared by peanut shell powder be 80%, wheatfeed or fine rice bran shared by weight be 20%；Then regulation compound is aqueous
Measure as 60%, then be loaded into seed bottle or polypropylene plastics bacterium bag, sealed with cotton plug, cooled down after 121 DEG C of sterilizing 2h,
In the ganoderma lucidum slant strains for accessing a block length 2cm, width 1cm under aseptic condition on its surface, then in 25 DEG C, relative humidity 60-
Light culture is carried out under conditions of 70%, preservation at the ganoderma lucidum solid spawn, 4 DEG C is made after mycelia material feeding is complete.
The ganoderma lucidum solid spawn can be directly used for the liquid deep layer fermenting of ganoderma lucidum, i.e., aseptically by the ganoderma lucidum
Solid spawn is smashed to pieces, is inoculated in liquid fermentation medium and carries out shake flask fermentation or ferment tank；The liquid fermentation and culture
Contain 30 g/L glucose, 20 g/L corn flour, 4 g/L peptones, 1.0 g/L potassium dihydrogen phosphates, 0.5 g/L magnesium sulfate in base.
When wherein, using shake flask fermentation, 0.25g ganoderma lucidum solid spawns are inoculated with every 100mL liquid fermentation mediums；It is sent out
Ferment condition is：1 day is stood at 28 DEG C, then with 160r/min rotating speed culture 5 days.
During using ferment tank, 1.5g ganoderma lucidum solid spawns are inoculated with every 1L liquid fermentation mediums；Its fermentation condition
For：28 DEG C, the vvm of throughput 0.5,120 ~ 160r/min of rotating speed, pH it is natural under the conditions of cultivate 5 ~ 6 days.
The beneficial effects of the present invention are：
（1）Solid spawn of the present invention, can repeatedly, for a long time in preservation at 4 DEG C（In 6 months）Use, its fermenting property without substantially under
Drop；And it is using peanut shell discarded object etc. as raw material, low is required to production equipment, makes strain preparation cost low, is conducive to industry
Change and prepare.
（2）Compared with traditional bacterium ball-type liquid spawn, the aseptic condition of solid spawn of the present invention is easy to control and maintains, and can drop
Low fermentation pollution rate；And it occupies little space, easy long term storage and transport；
（3）The present invention makes it easy after being inoculated with liquid fermentation medium using powdered compost as the growth substrate of strain
It is scattered, so as to dramatically increase Fermentative growth point, accelerate fermenting speed, so that biomass and the production of exocellular polysaccharide isoreactivity composition
Amount is significantly increased.
Embodiment
The invention provides a kind of ganoderma lucidum solid spawn and its application process in ganoderma lucidum liquid submerged fermentation, below it is real
The application method that example further describes the present invention is applied, but these embodiments are only normative, not to the scope of the present invention structure
Into any limitation.In addition, without departing from the spirit and scope of the invention can be to the details and form of technical solution of the present invention
Modify or replace, but these modifications and replacement are still in the scope of the present invention.
Contain 30 g/L glucose, 20 g/L corn flour, 4 g/L peptones, 1.0 g/L phosphorus in liquid fermentation medium used
Acid dihydride potassium, 0.5 g/L magnesium sulfate
Embodiment 1 uses the liquid shake-flask fermentation technique of ganoderma lucidum solid spawn
1）Peanut shell powder and wheatfeed are crushed using medicinal herb grinder, by both by weight 8:2 mixing；
2）The water content for adjusting compound is 60%, then takes 100g to be fitted into 250 mL triangular flasks, compost is slightly compacted
（Certain voidage is left, with profit ventilation）, sealed with cotton plug；
3）By compost in 121 DEG C of sterilizing 2h；
4）After compost cooling after, under aseptic condition its surface access a block length 2cm, width 1cm ganoderma lucidum slant strains, so
Light culture is carried out under conditions of 25 DEG C, relative humidity 60-70%, ganoderma lucidum solid spawn is made after mycelia material feeding is complete；
5）Aseptically ganoderma lucidum solid spawn is smashed to pieces with sterile spoon, takes 0.2 g to be inoculated in equipped with 80 mL liquid fermentations
In 250 mL shaking flasks of culture medium, in standing 1 day at 28 DEG C, then with 160r/min rotating speed culture 5 days.
Fermentation ends collect ganoderma lucidum mycelium, analysis exocellular polysaccharide and biomass.As a result show, gained ganoderma lucidum mycelium
Biomass reaches 15.2 g/L, and exocellular polysaccharide content is 1.12 g/L；And using traditional Liquid Strain of Ganoderma Lucidum to be fermented, it is given birth to
Object amount is only 9.1 g/L, and exocellular polysaccharide content is the biomass and exocellular polysaccharide of 0.76 g/L, i.e. the present embodiment compared with prior art
Content has been respectively increased 67% and 47.4%.
Embodiment 2 uses the fermentor liquid submerged fermentation of ganoderma lucidum solid spawn
1）Peanut shell powder and fine rice bran are crushed using medicinal herb grinder, by both by weight 8:2 mixing；
2）The water content for adjusting compound is 60%, then takes 300g to be fitted into 1 L triangular flasks, compost is slightly compacted（Stay
There is certain voidage, with profit ventilation）, sealed with cotton plug；
3）By compost in 121 DEG C of sterilizing 2h；
4）After compost cooling after, under aseptic condition its surface access a block length 2cm, width 1cm ganoderma lucidum slant strains, so
Light culture is carried out under conditions of 25 DEG C, relative humidity 60-70%, ganoderma lucidum solid spawn is made after mycelia material feeding is complete；
5）Aseptically ganoderma lucidum solid spawn is smashed to pieces with sterile spoon, takes 10.5 g to be inoculated in equipped with 7 L liquid fermentations
In 10 L fermentation tanks in culture medium, press 0.1 MPa, the vvm of throughput 0.5, rotating speed 160r/min, pH natural in 28 DEG C, tank
Under the conditions of cultivate 6 days.
Fermentation ends collect ganoderma lucidum mycelium, analysis exocellular polysaccharide and biomass.As a result show, gained ganoderma lucidum mycelium
Biomass reaches 14.3 g/L, and exocellular polysaccharide content is 0.91 g/L；And using traditional Liquid Strain of Ganoderma Lucidum to be fermented, it is given birth to
Object amount is 11.4 g/L, and exocellular polysaccharide content is the biomass and exocellular polysaccharide of 0.68 g/L, i.e. the present embodiment compared with prior art
25.4% and 33.8% is respectively increased in content.
Embodiment 3 uses the ganoderma lucidum liquid shake flask fermentation of long term storage solid spawn
1）Peanut shell powder and wheatfeed are crushed using medicinal herb grinder, by both by weight 8:2 mixing；
2）The water content for adjusting compound is 60%, then takes 100g to be fitted into 250 mL triangular flasks, compost is slightly compacted
（Certain voidage is left, with profit ventilation）, sealed with cotton plug；
3）By compost in 121 DEG C of sterilizing 2h；
4）After compost cooling after, under aseptic condition its surface access a block length 2cm, width 1cm ganoderma lucidum slant strains, so
Light culture is carried out under conditions of 25 DEG C, relative humidity 60-70%, ganoderma lucidum solid spawn is made after mycelia material feeding is complete, in
Preservation in 4 DEG C of ice chests；
5）Aseptically gone bail for the g of ganoderma lucidum solid spawn 0.2 hidden 3 months with sterile spoon, is inoculated in equipped with 80 mL liquid
In 250 mL shaking flasks of body fermentation medium, in standing 1 day at 28 DEG C, then with 160r/min rotating speed culture 5 days.
Fermentation ends collect ganoderma lucidum mycelium, analysis exocellular polysaccharide and biomass.As a result show, gained ganoderma lucidum mycelium
Biomass reaches 14.6 g/L, and exocellular polysaccharide content is 1.04 g/L；And using traditional Liquid Strain of Ganoderma Lucidum to be fermented, it is given birth to
Object amount is only 9.3 g/L, and exocellular polysaccharide content is the biomass and exocellular polysaccharide of 0.80 g/L, i.e. the present embodiment compared with prior art
Content has been respectively increased 56.7% and 30%.
Embodiment 4 uses long term storage ganoderma lucidum solid spawn and its fermentor liquid deep layer fermenting process
1）Peanut shell powder and wheatfeed are crushed using medicinal herb grinder, by both by weight 8:2 mixing；
2）The water content for adjusting compound is 60%, then takes 300g to be fitted into 1L triangular flasks, compost is slightly compacted（Stay
There is certain voidage, with profit ventilation）, sealed with cotton plug；
3）By compost in 121 DEG C of sterilizing 2h；
4）After compost cooling after, under aseptic condition its surface access a block length 2cm, width 1cm ganoderma lucidum slant strains, so
Light culture is carried out under conditions of 25 DEG C, relative humidity 60-70%, ganoderma lucidum solid spawn is made after mycelia material feeding is complete, in
Preservation in 4 DEG C of ice chests；
5）Aseptically the ganoderma lucidum solid spawn of preservation 4 months is smashed to pieces with sterile glass rod, takes 10.5 g to be inoculated in equipped with 7
In 10 L fermentation tanks of L liquid fermentation mediums, 28 DEG C, tank press 0.1 MPa, the vvm of throughput 0.5, rotating speed 120r/min,
Cultivated 5 days under the conditions of pH is natural.
Fermentation ends collect ganoderma lucidum mycelium, analysis exocellular polysaccharide and biomass.As a result show, gained ganoderma lucidum mycelium
Biomass reaches 14.8 g/L, and exocellular polysaccharide content is 0.82 g/L；And using traditional Liquid Strain of Ganoderma Lucidum to be fermented, it is given birth to
Object amount is 11.7 g/L, and exocellular polysaccharide content is the biomass and exocellular polysaccharide of 0.75 g/L, i.e. the present embodiment compared with prior art
26.5% and 9% has been respectively increased in content.
The foregoing is only presently preferred embodiments of the present invention, all equivalent changes done according to scope of the present invention patent with
Modification, should all belong to the covering scope of the present invention.
Claims (6)
1. a kind of ganoderma lucidum solid spawn, it is characterised in that：After peanut shell powder and wheatfeed or fine rice bran are crushed by a certain percentage
Mixing, the water content for then adjusting compound is 60%, then is loaded into seed bottle or polypropylene plastics bacterium bag, uses cotton plug
Sealing, after sterilized, cooling, in the ganoderma lucidum slant strains that a block length 2cm, width 1cm are accessed under aseptic condition, then in 25 DEG C,
Light culture is carried out under conditions of relative humidity 60-70%, the ganoderma lucidum solid spawn is made after mycelia material feeding is complete.
2. ganoderma lucidum solid spawn according to claim 1, it is characterised in that：In terms of dry weight, in compound shared by peanut shell powder
Weight be 80%, wheatfeed or fine rice bran shared by weight be 20%.
3. a kind of application of ganoderma lucidum solid spawn as claimed in claim 1 in ganoderma lucidum liquid submerged fermentation, it is characterised in that：
The ganoderma lucidum solid spawn is smashed to pieces under aseptic condition, is inoculated in liquid fermentation medium and carries out shake flask fermentation or fermentation tank hair
Ferment.
4. application of the ganoderma lucidum solid spawn in ganoderma lucidum liquid submerged fermentation according to claim 3, it is characterised in that：Institute
State in liquid fermentation medium containing 30 g/L glucose, 20 g/L corn flour, 4 g/L peptones, 1.0 g/L potassium dihydrogen phosphates,
0.5 g/L magnesium sulfate.
5. application of the ganoderma lucidum solid spawn in ganoderma lucidum liquid submerged fermentation according to claim 3, it is characterised in that：Using
During shake flask fermentation, 0.25g ganoderma lucidum solid spawns are inoculated with every 100mL liquid fermentation mediums；
Its fermentation condition is：1 day is stood at 28 DEG C, then with 160r/min rotating speed culture 5 days.
6. application of the ganoderma lucidum solid spawn in ganoderma lucidum liquid submerged fermentation according to claim 3, it is characterised in that：Using
During ferment tank, 1.5g ganoderma lucidum solid spawns are inoculated with every 1L liquid fermentation mediums；
Its fermentation condition is：Cultivated 5 ~ 6 days under conditions of 28 DEG C, the vvm of throughput 0.5,120 ~ 160r/min of rotating speed.
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Cited By (3)
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| CN107586727A (en) * | 2017-10-26 | 2018-01-16 | 湖南和平生物科技有限公司 | A kind of fermentation process of ganoderma lucidum mycelium |
| CN109777847A (en) * | 2019-03-01 | 2019-05-21 | 宁德师范学院 | The method of body cell is affine ganoderma strain to common fermentation production strong anti-oxidative activity polysaccharide |
| CN109845586A (en) * | 2019-02-15 | 2019-06-07 | 宁德师范学院 | A kind of flammulina velutipes liquid strains preparation method of the growing point of mycelia containing high quantity |
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| CN109845586A (en) * | 2019-02-15 | 2019-06-07 | 宁德师范学院 | A kind of flammulina velutipes liquid strains preparation method of the growing point of mycelia containing high quantity |
| CN109777847A (en) * | 2019-03-01 | 2019-05-21 | 宁德师范学院 | The method of body cell is affine ganoderma strain to common fermentation production strong anti-oxidative activity polysaccharide |
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