CN104782384A - Method for recovering ganoderma lucidum solid strain into liquid strain - Google Patents
Method for recovering ganoderma lucidum solid strain into liquid strain Download PDFInfo
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- CN104782384A CN104782384A CN201510119714.9A CN201510119714A CN104782384A CN 104782384 A CN104782384 A CN 104782384A CN 201510119714 A CN201510119714 A CN 201510119714A CN 104782384 A CN104782384 A CN 104782384A
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- ganoderma lucidum
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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Abstract
A method for recovering a ganoderma lucidum solid strain into a liquid strain particularly includes the following steps that the raw materials of a culture medium are composed of corn flour and saw dust, and the culture medium is subpackaged into a triangular bottle and then sterilized according to a common method; the ganoderma lucidum solid strain is put in the triangular bottle according to a common method, and the ganoderma lucidum mycelia is cultured to grow full of the culture medium; sterilized water is poured into the triangular bottle, the strain the bottle is smashed, and then the ganoderma lucidum solid strain is recovered into the liquid strain. A traditional ganoderma lucidum liquid strain needs a professional fermentation tank. According to the method, only the common triangular bottle is needed, investment is small, and risks are low. The safety coefficient of the method is improved by N times compared with the traditional liquid strain. In the implementation process of the method, the culture medium does not contain sugar, thereby being not prone to being polluted, and the culture medium is easy to store and convenient to convey. By means of the method, the prepared ganoderma lucidum liquid strain has the same effect as the traditional ganoderma lucidum liquid strain, but the investment is reduced by nearly 50 times.
Description
Technical field
The invention belongs to biological technical field, be specifically related to a kind of glossy ganoderma solid spawn and be reduced to liquid spawn.
Background technology
" liquid spawn " is with liquid nutrient medium, in biological fermentation tank, and the edible fungus species of the liquid form produced by deep drainpipe (the shape liquid fermentation of liquid spawn) technology.
" liquid " refers to medium physical state, and " Submerged liquid culturation " is exactly fermentation engineering." the liquid production of hybrid seeds " essence utilizes biological fermentation engineering to produce liquid spawn, replaces tradition, the simple solid production of hybrid seeds; Utilize biofermentation principle, provide a best nutrition, acid-base value, temperature, oxygen-supplying amount to mycelial growth, mycelia is grown fast, expand rapidly numerous, reach certain bacterium ball quantity in the short time, complete a fermentation period, namely cultivate complete.
Liquid spawn is applied to the production of edible mushroom, for edible mushroom industry from the very complicated traditional mode of production, the cycle is long, cost is high, by rule of thumb, spell labour, handicraft workshop formula to automation, standardization, scale upgrading be significant.During liquid spawn access solid culture medium, have again mobility, easily dispersion, sprout soon, send out the features such as bacterium point is many, preferably resolve in seeded process and sprout slow, the easy problem polluted, bacterial classification can carry out suitability for industrialized production.Liquid spawn nongraded system, can be used for doing female kind and produce original seed, can also be directly used in cultivation sowing as cultivated species.Liquid spawn also has following eight large advantages:
(1) cost is low: breeding cost is a thirtieth of solid;
(2) time is short: the production of hybrid seeds time is 1/10 of solid;
(3) sprout soon: inoculate sprouting in latter 6 hours, within 24 hours, mycelia is covered with inoculating surfaces;
(4) growth is fast: the cultivation bacteria time shortens 1/2 than solid;
(5) purity is high: the complete airtight operation of equipment, and bacterial classification purity is high, energetic;
(6) pollute less: bacterial classification germination physiology is fast, and miscellaneous bacteria does not almost infect chance;
(7) cell age is short: it is consistent that bacterium wraps lower cell age, goes out that ear is neat, output is high;
(8) automation: button operation, automaticity is high.
At present, preparation technology's flow process of the liquid spawn of glossy ganoderma is as follows: the cleaning of the preparation → fermentation tank of triangle shaking flask bacterial classification (one-level mother plant) and inspection → medium preparation → material loading tinning → medium sterilization → cooling down → access one-level mother plant (triangle shaking flask bacterial classification) → fermented and cultured → bacterial classification and export and inoculate.
Produce Liquid Strain of Ganoderma Lucidum with said method, not only link is many, complicated operation, and the cycle is long.Also need the fermentation of specialty to fill with could implement simultaneously.Have high input, risk is high.
Summary of the invention
The object of the invention is overcome the deficiencies in the prior art and provide brand-new a kind of glossy ganoderma solid spawn to be reduced to liquid spawn.Specifically comprise: culture medium raw material is made up of corn flour and wood chip, be sub-packed in by medium in triangular flask, then carry out disinfection sterilizing according to a conventional method; Then according to a conventional method by lucidum strain kind access triangular flask, and be cultured to Ganoderma lucidum mycelium and cover with medium; Finally pour into inside triangular flask with sterile water, then the Cordycepps in bottle is smashed to pieces, then glossy ganoderma solid spawn is reduced to liquid spawn.Traditional Liquid Strain of Ganoderma Lucidum needs the fermentation of specialty to fill with, and implements the present invention, and only need conventional triangular flask, less investment, risk is low.And enforcement the present invention, improve N doubly than doing traditional liquid spawn safety coefficient.The present invention is in implementation process, because medium is not containing sugar, so be not easy to pollute, and preserve easily, transport also facilitates.By implementing the present invention, prepared Liquid Strain of Ganoderma Lucidum out and traditional Liquid Strain of Ganoderma Lucidum are the same effects, but input ratio really omits 50 times nearly.
The present invention is achieved through the following technical solutions:
A kind of glossy ganoderma solid spawn is reduced to liquid spawn, is to comprise the steps: in technical scheme
1, prepare medium: raw material is made up of the corn flour of 50% and the wood chip of 50%, two kinds of raw materials mixed, then admix appropriate water the moisture of mixed material is adjusted to 45 ~ 50% and medium.
2, bottling sterilizing: be sub-packed in by medium in triangular flask, medium charge weight is 1/3rd of triangular flask, and then carry out disinfection sterilizing according to a conventional method.
3, cultivation is inoculated: according to a conventional method by lucidum strain kind access triangular flask, and be cultured to Ganoderma lucidum mycelium and cover with medium.
4, sterile water is prepared: conventionally prepare free of contamination sterile water;
5, liquid spawn is prepared: sterile water is poured into inside the triangular flask covering with Ganoderma lucidum mycelium in gnotobasis, then smashed to pieces by the bacterial classification in bottle, then glossy ganoderma solid spawn is reduced to liquid spawn.
The present invention both had the following advantages:
1, it is unique that glossy ganoderma solid spawn proposed by the invention is reduced to its method and measure of liquid spawn, and technique is simple, easily implements.
2, traditional ganoderma lucidum liquid Cordycepps needs the fermentation of specialty to fill with, and implements the present invention, and only need conventional triangular flask, less investment, risk is low.
3, by implementing the present invention, improve N doubly than doing traditional liquid spawn safety coefficient.
4, the present invention is in implementation process, because medium is not containing sugar, so be not easy to pollute, and preserve easily, transport also facilitates.
5, by implementing the present invention, prepared Liquid Strain of Ganoderma Lucidum out and traditional liquid spawn are the same effects, but input ratio really economizes 50 times nearly.
6, implement the present invention, be more suitable for Liquid Strain of Ganoderma Lucidum batch production, scale, intensive manufacture.Be worth promoting.
Embodiment
Below in conjunction with embodiment, method of the present invention is further illustrated.
Plant glossy ganoderma solid spawn and be reduced to liquid spawn, embodiment is as follows:
1, prepare medium: raw material is made up of the corn flour of 50% and the wood chip of 50%, two kinds of raw materials mixed, then admix appropriate water the moisture of mixed material is adjusted to 45 ~ 50% and medium.
2, bottling sterilizing: be sub-packed in by medium in triangular flask, medium charge weight is 1/3rd of triangular flask, and then carry out disinfection sterilizing according to a conventional method.
3, cultivation is inoculated: according to a conventional method by lucidum strain kind access triangular flask, and be cultured to Ganoderma lucidum mycelium and cover with medium.
4, sterile water is prepared: conventionally prepare free of contamination sterile water;
5, liquid spawn is prepared: sterile water is poured into inside the triangular flask covering with Ganoderma lucidum mycelium in gnotobasis, then smashed to pieces by the bacterial classification in bottle, then glossy ganoderma solid spawn is reduced to liquid spawn.
Claims (1)
1. glossy ganoderma solid spawn is reduced to a liquid spawn, it is characterized in that comprising the steps:
(1) prepare medium: raw material is made up of the corn flour of 50% and the wood chip of 50%, two kinds of raw materials mixed, then admix appropriate water the moisture of mixed material is adjusted to 45 ~ 50% and medium;
(2) bottling sterilizing: be sub-packed in by medium in triangular flask, medium charge weight is 1/3rd of triangular flask, and then carry out disinfection sterilizing according to a conventional method;
(3) cultivation is inoculated: according to a conventional method by lucidum strain kind access triangular flask, and be cultured to Ganoderma lucidum mycelium and cover with medium;
(4) sterile water is prepared: conventionally prepare free of contamination sterile water;
(5) liquid spawn is prepared: sterile water is poured into inside the triangular flask covering with Ganoderma lucidum mycelium in gnotobasis, then smashed to pieces by the bacterial classification in bottle, then glossy ganoderma solid spawn is reduced to liquid spawn.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201510119714.9A CN104782384A (en) | 2015-03-19 | 2015-03-19 | Method for recovering ganoderma lucidum solid strain into liquid strain |
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| CN201510119714.9A CN104782384A (en) | 2015-03-19 | 2015-03-19 | Method for recovering ganoderma lucidum solid strain into liquid strain |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105684731A (en) * | 2016-01-28 | 2016-06-22 | 孙继勇 | Cut-log type ganoderma lucidum culture method in imitating wild environment |
| CN105684730A (en) * | 2016-01-28 | 2016-06-22 | 孙继勇 | Ganoderma lucidum culture method in imitating wild environment |
| CN106399125A (en) * | 2016-09-09 | 2017-02-15 | 深圳市泽青源科技开发服务有限公司 | Preparation method and application of fungal suspension |
| CN107177515A (en) * | 2017-07-21 | 2017-09-19 | 宁德师范学院 | A kind of ganoderma lucidum solid spawn and its application in ganoderma lucidum liquid submerged fermentation |
| CN109220559A (en) * | 2018-09-30 | 2019-01-18 | 上海市农业科学院 | A kind of production method and growth condition of Stropharia rugoso-annulata reduction strain |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102715014A (en) * | 2012-06-25 | 2012-10-10 | 浙江常山荣昇食用菌有限公司 | Preparation method for liquefied strain of edible mushroom |
| CN103583233A (en) * | 2013-11-11 | 2014-02-19 | 黄秀英 | Preparation method for reduction type liquid spawn |
| CN104025903A (en) * | 2014-05-22 | 2014-09-10 | 河北绿地久乐生物科技股份有限公司 | Liquid fermentation medium for culturing edible mushroom liquid microbial strains and liquid microbial strain preparation method |
| CN104261942A (en) * | 2014-09-28 | 2015-01-07 | 永和县旺达食用菌有限责任公司 | Pleurotus nebrodensis liquid strain culture medium and propagation method of strain |
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2015
- 2015-03-19 CN CN201510119714.9A patent/CN104782384A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102715014A (en) * | 2012-06-25 | 2012-10-10 | 浙江常山荣昇食用菌有限公司 | Preparation method for liquefied strain of edible mushroom |
| CN103583233A (en) * | 2013-11-11 | 2014-02-19 | 黄秀英 | Preparation method for reduction type liquid spawn |
| CN104025903A (en) * | 2014-05-22 | 2014-09-10 | 河北绿地久乐生物科技股份有限公司 | Liquid fermentation medium for culturing edible mushroom liquid microbial strains and liquid microbial strain preparation method |
| CN104261942A (en) * | 2014-09-28 | 2015-01-07 | 永和县旺达食用菌有限责任公司 | Pleurotus nebrodensis liquid strain culture medium and propagation method of strain |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105684731A (en) * | 2016-01-28 | 2016-06-22 | 孙继勇 | Cut-log type ganoderma lucidum culture method in imitating wild environment |
| CN105684730A (en) * | 2016-01-28 | 2016-06-22 | 孙继勇 | Ganoderma lucidum culture method in imitating wild environment |
| CN106399125A (en) * | 2016-09-09 | 2017-02-15 | 深圳市泽青源科技开发服务有限公司 | Preparation method and application of fungal suspension |
| CN107177515A (en) * | 2017-07-21 | 2017-09-19 | 宁德师范学院 | A kind of ganoderma lucidum solid spawn and its application in ganoderma lucidum liquid submerged fermentation |
| CN109220559A (en) * | 2018-09-30 | 2019-01-18 | 上海市农业科学院 | A kind of production method and growth condition of Stropharia rugoso-annulata reduction strain |
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