CN104261942A - Pleurotus nebrodensis liquid strain culture medium and propagation method of strain - Google Patents
Pleurotus nebrodensis liquid strain culture medium and propagation method of strain Download PDFInfo
- Publication number
- CN104261942A CN104261942A CN201410504385.5A CN201410504385A CN104261942A CN 104261942 A CN104261942 A CN 104261942A CN 201410504385 A CN201410504385 A CN 201410504385A CN 104261942 A CN104261942 A CN 104261942A
- Authority
- CN
- China
- Prior art keywords
- liquid
- solid
- pleurotus nebrodensis
- parts
- culture medium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Mycology (AREA)
- Environmental Sciences (AREA)
- Mushroom Cultivation (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a pleurotus nebrodensis liquid strain culture medium, comprising the following raw materials in parts by weight: 4-4.5 parts of bran, 2-2.5 parts of corn flour, 1.2-1.6 parts of soybean cake powder, 1.5-2 parts of glucose, 0.1-0.12 part of peptone, 0.1-0.12 part of KH2PO4, 0.05-0.06 part of MgSO4 and 0.01 part of VB1 in 100 parts of water. A propagation method of a pleurotus nebrodensis strain comprises the following steps: preparing a liquid culture medium; adjusting the pH value of the liquid culture medium; sterilizing, and inoculating solid strain seeds; cultivating and preparing a solid fungus bag medium; cultivating solid strains; and inoculating and cultivating a fruiting fungus bag. The liquid culture medium disclosed by the invention is abundant in raw material source, and low in price; and due to a solid-liquid-solid production mode, compared with a traditional complete solid culture method, hyphae are even in growth speed in the fungus bag, regular and consistent in fruiting, even in sporocarp size, good in mushroom shape, good in quality, short in fermentation cycle, high in yield, and high in economic benefits, and thus the method is very suitable for popularization in small and medium-sized enterprises and peasant households.
Description
Technical field
The present invention relates to bacterium culture medium and bacterial classification expanding propagation technical field, particularly relate to a kind of efficient, Pleurotus nebrodensis liquid spawn culture medium of less contamination and the expanding propagation method of bacterial classification.
Background technology
Pleurotus nebrodensis is a kind of dietotherapeutic and the very high edible mushrooms of nutritive value.The pharmaceutical value of Pleurotus nebrodensis has disappear long-pending, desinsection, antibechic, the effect such as anti-inflammatory and control gynecological tumor.According to surveying and determination, the protein content of Pleurotus nebrodensis accounts for 20% of dry mushroom, containing 17 seed amino acids, and physiologically active substance and the several mineral materials such as multivitamin, inorganic salt, fungus polysaccharide and VITAMIN, have and regulate human body physiological equilibrium, strengthen the effect of immune function of human body.The mushroom body colour pool of Pleurotus nebrodensis is pure white, meat is thin
Greasy, delicious flavour, is described as " bolete on grassland ", quite by the favor of human consumer.
In the prior art, the bacterial classification of edible mushrooms has solid spawn and liquid spawn, traditional method expands numerous with solid form strain inoculation, liquid spawn is low with cost, incubation time is short, send out the advantages such as bacterium is fast, biological transformation ratio is high, bacterial classification purity is high, in modern times extensive edible mushrooms cultivation, take vaccination ways as the leading factor, this is also the trend of mushroom industry batch production technical development.But the practical situation that China Pleurotus nebrodensis is cultivated are small scale and dispersion, and this management mode can not short in a twinkling in be able to basic change.Therefore, study a kind of be suitable for the current existing Pleurotus nebrodensis training mode of China bacterium culture medium and preparation method have important practical significance.
Summary of the invention
The present invention is directed to prior art Problems existing, there is provided a kind of and be suitable for middle and small scale cultivation Pleurotus nebrodensis liquid spawn culture medium and the expanding propagation method of bacterial classification, namely introduce solid spawn seed and add " solid, liquid, solid " pattern that liquid spawn expanding propagation adds cultivar solid spawn.
The present invention is achieved through the following technical solutions.
Pleurotus nebrodensis liquid spawn culture medium is the raw material composition adding following mass parts in 100 parts of water, wheat bran 4-4.5 part, Semen Maydis powder 2-2.5 part, soybean cake powder 1.2-1.6 part, glucose 1.5-2 part, peptone 0.1-0.12 part, KH
2pO
40.1-0.12 part, MgSO
40.05-0.06 part, VB
10.01 part.
In the cultivation of Pleurotus nebrodensis bacterial strain, have multiple carbon nitrogen source to utilize, but find that wherein carbon source is best with wheat bran or Semen Maydis powder through applicant's years of researches, nitrogenous source take soybean cake powder as the best.
The expanding propagation method of Pleurotus nebrodensis strain, comprises the steps:
(1) preparation of liquid nutrient medium is by wheat bran 4-4.5 part, Semen Maydis powder 2-2.5 part, soybean cake powder 1.2-1.6 part, glucose 1.5-2 part, peptone 0.1-0.12 part, KH
2pO
40.1-0.12 part, MgSO
40.05-0.06 part, VB
10.01 part adds successively in 100 parts of water, from less to more until stir in stirring;
(2) pH value of regulates liquid substratum is 6 ~ 6.5;
(3) ready medium liquid is poured in seeding tank by sterilizing, sends in sterilizing device and carries out at least 30 points
The sterilizing of clock;
(4) access solid spawn seed and cultivate the Pleurotus nebrodensis solid spawn seed accessing outsourcing after the medium liquid after sterilizing is cooled to room temperature, then cultivation liquid is adjusted to 24-25 DEG C, within venting pressure >=0.3Kg/cm, fermentation culture 7-8 days, Pleurotus nebrodensis liquid culture bacterial classification can be obtained;
(5) while preparation liquid culture bacterial classification, the preparation work of the adjustment of solid bacterium bag culture medium batching, heap fermentation, pH value and moisture, pack sterilizing, cooling bacterium bag is carried out;
(6) solid spawn is cultivated in the liquid spawn (4) made aseptically use aseptic inoculation rifle to access fast part bacterium bag that (5) make, select to grow fine as solid state cultivation bacterial classification, find that there is managing as fruiting bag of pollution;
(7) solid spawn that (6) make aseptically to be accessed in the bacterium bag that (5) make, by the cultivation of Pleurotus nebrodensis conventional culture conditions, to fruiting by the inoculation and cultivating of fruiting bacterium bag.
Beneficial effect of the present invention
(1) liquid medium starting material that adopts of the present invention, abundance, cheap.The Pleurotus nebrodensis liquid spawn mycelial growth adopting liquid nutrient medium of the present invention to turn out fast, sturdy energetic, hyphae content is high, postvaccinal bacterial classification germination rate is good, fermentation period is short, yield rate is high.
(2) the present invention is according to current medium and small sized enterprises of China and the working condition of peasant household and the use habit to solid spawn, have employed the production model that " Gu solid-liquid-" combines, namely spawn culture is required that the strictest seed culture is produced by large-lot producer or R&D institution, medium and small sized enterprises and peasant household directly purchase seed, medium and small sized enterprises and peasant household directly carry out the cultivation of liquid spawn, first be inoculated in cultivar after liquid fungus seed is good to cultivate, and then cultured pollution-free, that mycelia is healthy and strong High-quality Cultivation kind is inoculated fruiting bag as solid spawn.The advantage of this production model: one is the rational division of labor in society achieving Cultivation of Pleurotus nebrodensis according to the advantage of constituent parts, avoid and pursue backward production model small and comprehensive, reduce the production cost of seed culture, solid seed can be stored, portable, handled easily; Two is because liquid spawn quality not easily differentiates general peasant household, and high to the quality degree of awareness of cultivar, therefore, cultivar inoculation fruiting bag peasant household is relieved, the use habit of peasant household can be met, more can avoid because liquid spawn quality or the improper big area that causes of operation link are lost.
(3) the complete solid culture method high financial profit that the inventive method is more traditional, show: one is that liquid spawn mycelium bacterial classification cost only has 0.02 ~ 0.03 yuan, and traditional solid spawn production cost is more than 0.3 yuan, makes bacterial classification cost and reduce 10 times; Two is that liquid spawn mycelium passes through screening, purifies, and equipment runs completely under gnotobasis condition in process of production, makes bacterium property purer, purity is higher, and traditional frequent expansion of solid spawn production switches through generation, mycelia vigor is reduced greatly, very easily pollutes; Three is with short production cycle, liquid spawn mycelium from submerged fermentation to access bacterium bag after mycelia cover with bacterium bag and only need about 25d, only need 1.5 ~ 2 months to fruiting, and traditional bacterial classification is produced and is at least needed more than 3 months from the production of hybrid seeds to fruiting one-period; Four is quality betters, and the cultivating bag cell age of liquid spawn is short, and mycelia speed of growth in bacterium bag is even, fruiting neat and consistent, and sporophore size is even, and mushroom type is good, quality is good, and yield rate is high.
Embodiment
embodiment 1
Certain Edible Fungi cooperative society, the liquid fungus seed equipment of existing 500L, the expanding propagation method making Pleurotus nebrodensis liquid spawn culture medium and bacterial classification is as follows;
(1) wheat bran of 16 kilograms, the Semen Maydis powder of 10 kilograms, the soybean cake powder of 4.8 kilograms, the glucose of 8 kilograms, the peptone of 0.4 kilogram, the KH of 0.48 kilogram is taken first respectively
2pO
4, the MgSO of 0.2 kilogram
4, the VB of 0.04 kilogram
1by weight, add successively in the water of 400L in stirring from less to more, after regulating the pH value to 6 of mixed solution, proceed in liquid spawn fermentation tank, sterilizing, the Pleurotus nebrodensis solid spawn seed of outsourcing is accessed after the medium liquid after sterilizing is cooled to room temperature, then cultivation liquid is adjusted to 24-25 DEG C, venting pressure>=0.3Kg/cm, namely fermentation culture obtains the bacterial classification of Pleurotus nebrodensis liquid culture for 7 days.
(2) while preparation liquid culture bacterial classification, carry out the preparation work of the adjustment of solid bacterium bag culture medium batching, heap fermentation, pH value and moisture, pack sterilizing, cooling bacterium bag, then aseptic inoculation rifle is aseptically used by the liquid spawn that (1) makes to access in part bacterium bag fast, select grow fine as solid state cultivation bacterial classification, find that there is managing as fruiting bag of pollution; Again the solid spawn of making is aseptically accessed and (in other bacterium bags, cultivate, to fruiting by Pleurotus nebrodensis conventional culture conditions.
embodiment 2
The liquid fungus seed equipment of the existing 100L of certain peasant household, the expanding propagation method making Pleurotus nebrodensis liquid spawn culture medium and bacterial classification is as follows;
(1) wheat bran of 4.5 kilograms, the Semen Maydis powder of 2 kilograms, the soybean cake powder of 1.6 kilograms, the glucose of 1.5 kilograms, the peptone of 0.12 kilogram, the KH of 0.1 kilogram is taken first respectively
2pO
4, the MgSO of 0.06 kilogram
4, the VB of 0.01 kilogram
1by weight, add successively in the water of 100L in stirring from less to more, after regulating the pH value to 6.5 of mixed solution, proceed in liquid spawn fermentation tank, sterilizing, the Pleurotus nebrodensis solid spawn seed of outsourcing is accessed after the medium liquid after sterilizing is cooled to room temperature, then cultivation liquid is adjusted to 24-25 DEG C, venting pressure>=0.3Kg/cm, namely fermentation culture obtains the bacterial classification of Pleurotus nebrodensis liquid culture for 8 days.
(2) while preparation liquid culture bacterial classification, carry out the preparation work of the adjustment of solid bacterium bag culture medium batching, heap fermentation, pH value and moisture, pack sterilizing, cooling bacterium bag, then aseptic inoculation rifle is aseptically used by the liquid spawn that (1) makes to access in part bacterium bag fast, select grow fine as solid state cultivation bacterial classification, find that there is managing as fruiting bag of pollution; Again the solid spawn of making is aseptically accessed and (in other bacterium bags, cultivate, to fruiting by Pleurotus nebrodensis conventional culture conditions.
Claims (2)
1. a Pleurotus nebrodensis liquid spawn culture medium, is characterized in that the raw material composition adding following mass parts in 100 parts of water, wheat bran 4-4.5 part, Semen Maydis powder 2-2.5 part, soybean cake powder 1.2-1.6 part, glucose 1.5-2 part, peptone 0.1-0.12 part, KH
2pO
40.1-0.12 part, MgSO
40.05-0.06 part, VB
10.01 part.
2. the expanding propagation method of Pleurotus nebrodensis strain, comprises the steps:
(1) preparation of liquid nutrient medium is by wheat bran 4-4.5 part, Semen Maydis powder 2-2.5 part, soybean cake powder 1.2-1.6 part, glucose 1.5-2 part, peptone 0.1-0.12 part, KH
2pO
40.1-0.12 part, MgSO
40.05-0.06 part, VB
10.01 part adds successively in 100 parts of water, from less to more until stir in stirring;
(2) pH value of regulates liquid substratum is 6 ~ 6.5;
(3) ready medium liquid is poured in seeding tank by sterilizing, sends in sterilizing device and carries out at least 30 points
The sterilizing of clock;
(4) access solid spawn seed and cultivate the Pleurotus nebrodensis solid spawn seed accessing outsourcing after the medium liquid after sterilizing is cooled to room temperature, then cultivation liquid is adjusted to 24-25 DEG C, within venting pressure >=0.3Kg/cm, fermentation culture 7-8 days, Pleurotus nebrodensis liquid culture bacterial classification can be obtained;
(5) while preparation liquid culture bacterial classification, the preparation work of the adjustment of solid bacterium bag culture medium batching, heap fermentation, pH value and moisture, pack sterilizing, cooling bacterium bag is carried out;
(6) solid spawn is cultivated in the liquid spawn (4) made aseptically use aseptic inoculation rifle to access fast part bacterium bag that (5) make, select to grow fine as solid state cultivation bacterial classification, find that there is managing as fruiting bag of pollution;
(7) solid spawn that (6) make aseptically to be accessed in the bacterium bag that (5) make, by the cultivation of Pleurotus nebrodensis conventional culture conditions, to fruiting by the inoculation and cultivating of fruiting bacterium bag.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410504385.5A CN104261942A (en) | 2014-09-28 | 2014-09-28 | Pleurotus nebrodensis liquid strain culture medium and propagation method of strain |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410504385.5A CN104261942A (en) | 2014-09-28 | 2014-09-28 | Pleurotus nebrodensis liquid strain culture medium and propagation method of strain |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104261942A true CN104261942A (en) | 2015-01-07 |
Family
ID=52153564
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410504385.5A Pending CN104261942A (en) | 2014-09-28 | 2014-09-28 | Pleurotus nebrodensis liquid strain culture medium and propagation method of strain |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104261942A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104782384A (en) * | 2015-03-19 | 2015-07-22 | 黄秀英 | Method for recovering ganoderma lucidum solid strain into liquid strain |
| CN107258320A (en) * | 2017-06-27 | 2017-10-20 | 安徽诺亚农业有限公司 | A kind of Pleurotus nebrodensis high-yield cultivating method |
| CN109076883A (en) * | 2018-08-28 | 2018-12-25 | 重庆市药物种植研究所 | A kind of liquid spawn-solid state cultivation kind cultural method of the rotten edible mushroom of grass |
| CN110278828A (en) * | 2019-07-10 | 2019-09-27 | 新疆农业科学院植物保护研究所 | A kind of method of Pleurotus nebrodensis field child care on the spot |
| CN112341268A (en) * | 2020-10-29 | 2021-02-09 | 吴迪 | Culture solution for cultivating mushrooms and preparation method thereof |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1644011A (en) * | 2005-02-22 | 2005-07-27 | 余兆丰 | Secondary fermentation for culturing mycelium by binary microbial liquid |
| CN101085981A (en) * | 2006-06-08 | 2007-12-12 | 河南农业大学 | Edible mushroom liquid strain solidifying processing method |
| CN101147451A (en) * | 2007-11-06 | 2008-03-26 | 李程才 | Bailing mushroom field cultivating method using with mulch film to cover and cover soil |
| CN101647370A (en) * | 2009-08-24 | 2010-02-17 | 陈恒雷 | Standardized high-yield culture technique based on biological characteristics of asafetida mushroom |
| CN101755909A (en) * | 2009-12-14 | 2010-06-30 | 天津市林业果树研究所 | Making method of pleurotus edible fungus flavor yoghourt |
| CN101773050A (en) * | 2009-12-22 | 2010-07-14 | 唐山师范学院 | Method for rejuvenating and detoxifying edible fungal strain by using protoplast regeneration technology |
| CN102786338A (en) * | 2011-05-14 | 2012-11-21 | 郭长瑶 | Preparation method for pleurotus nebrodensis culture medium |
| CN102898207A (en) * | 2012-09-12 | 2013-01-30 | 崔学恒 | Liquid medium for cordyceps militaris strain, and preparation and application methods thereof |
| CN102964156A (en) * | 2012-08-30 | 2013-03-13 | 上海雪榕生物科技股份有限公司 | Culture medium formula and preparation method of Pleurotus nebrodensis liquid spawn |
-
2014
- 2014-09-28 CN CN201410504385.5A patent/CN104261942A/en active Pending
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1644011A (en) * | 2005-02-22 | 2005-07-27 | 余兆丰 | Secondary fermentation for culturing mycelium by binary microbial liquid |
| CN101085981A (en) * | 2006-06-08 | 2007-12-12 | 河南农业大学 | Edible mushroom liquid strain solidifying processing method |
| CN101147451A (en) * | 2007-11-06 | 2008-03-26 | 李程才 | Bailing mushroom field cultivating method using with mulch film to cover and cover soil |
| CN101647370A (en) * | 2009-08-24 | 2010-02-17 | 陈恒雷 | Standardized high-yield culture technique based on biological characteristics of asafetida mushroom |
| CN101755909A (en) * | 2009-12-14 | 2010-06-30 | 天津市林业果树研究所 | Making method of pleurotus edible fungus flavor yoghourt |
| CN101773050A (en) * | 2009-12-22 | 2010-07-14 | 唐山师范学院 | Method for rejuvenating and detoxifying edible fungal strain by using protoplast regeneration technology |
| CN102786338A (en) * | 2011-05-14 | 2012-11-21 | 郭长瑶 | Preparation method for pleurotus nebrodensis culture medium |
| CN102964156A (en) * | 2012-08-30 | 2013-03-13 | 上海雪榕生物科技股份有限公司 | Culture medium formula and preparation method of Pleurotus nebrodensis liquid spawn |
| CN102898207A (en) * | 2012-09-12 | 2013-01-30 | 崔学恒 | Liquid medium for cordyceps militaris strain, and preparation and application methods thereof |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104782384A (en) * | 2015-03-19 | 2015-07-22 | 黄秀英 | Method for recovering ganoderma lucidum solid strain into liquid strain |
| CN107258320A (en) * | 2017-06-27 | 2017-10-20 | 安徽诺亚农业有限公司 | A kind of Pleurotus nebrodensis high-yield cultivating method |
| CN109076883A (en) * | 2018-08-28 | 2018-12-25 | 重庆市药物种植研究所 | A kind of liquid spawn-solid state cultivation kind cultural method of the rotten edible mushroom of grass |
| CN110278828A (en) * | 2019-07-10 | 2019-09-27 | 新疆农业科学院植物保护研究所 | A kind of method of Pleurotus nebrodensis field child care on the spot |
| CN112341268A (en) * | 2020-10-29 | 2021-02-09 | 吴迪 | Culture solution for cultivating mushrooms and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103168613B (en) | Fast manufacture method for liquid strain | |
| CN103155785B (en) | Establishment method for brown mushroom three-level strain propagation system | |
| CN104261942A (en) | Pleurotus nebrodensis liquid strain culture medium and propagation method of strain | |
| CN104025903A (en) | Liquid fermentation medium for culturing edible mushroom liquid microbial strains and liquid microbial strain preparation method | |
| CN104761386B (en) | The compost and preparation method of a kind of utilization giant knotweed rhizome residue Xinbao mushroom culturing | |
| CN105441337A (en) | Preparation method of cultivated strain of tremella aurantialba | |
| CN102783356A (en) | Method for converting edible mushroom solid strain into liquid strain | |
| CN105110961A (en) | Liquid fermentation culture medium for shiitake mushrooms and method for producing shiitake mushrooms through the same | |
| CN105543104A (en) | Optimization method of solid culture medium for artificial acclimation and cultivation of wild Isaria cicadae Miquel | |
| CN102249752A (en) | Microbial fertilizer and preparation method and application thereof | |
| CN109566268A (en) | Effective original seed of a kind of gold ear and preparation method thereof | |
| CN102630483A (en) | Method for shortening cultivation period of needle mushroom during production | |
| CN105237248A (en) | Grifola frondosa production culture medium and application thereof | |
| CN101302127B (en) | Special fertilizer for M edible fungus and preparing method thereof | |
| CN103004453A (en) | Manufacturing method of edible fungi cultivar and culture medium manufacturing raw materials for edible fungi cultivar | |
| CN109526552A (en) | A kind of sclerotium industrial planting method of hickory chick | |
| CN103864537A (en) | Preparation method for morchella formula | |
| CN101717309A (en) | Culture medium for straw rotting edible fungi solid strain and method for preparing solid strain | |
| CN105925495A (en) | Highly-active Pichia pastoris powder and preparation method thereof | |
| CN103975763A (en) | Agrocybe cylindracea cultivation method | |
| CN108522147A (en) | The culture medium and cultural method of economical short cycle cultivation Pleurotus eryngii | |
| CN107867934A (en) | A kind of culture medium for being used for true pleurotus cornucopiae and white beech mushroom and its preparation method and application | |
| CN104838886A (en) | Industrial edible fungi cultivation method | |
| CN101828480B (en) | Pleurotus ferulae iarovization post-maturation low-temperature treatment artificial cultivation method | |
| CN104177182A (en) | Cultural method for producing cordyceps militaris fruiting body by use of corn cobs |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20150107 |
|
| RJ01 | Rejection of invention patent application after publication |