CN102786338A - Preparation method for pleurotus nebrodensis culture medium - Google Patents
Preparation method for pleurotus nebrodensis culture medium Download PDFInfo
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- CN102786338A CN102786338A CN2011101258214A CN201110125821A CN102786338A CN 102786338 A CN102786338 A CN 102786338A CN 2011101258214 A CN2011101258214 A CN 2011101258214A CN 201110125821 A CN201110125821 A CN 201110125821A CN 102786338 A CN102786338 A CN 102786338A
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Abstract
The invention relates to a preparation method for a pleurotus nebrodensis culture medium, and belongs to the technical field of edible fungi culture. According to the present invention, components in an medium formula and a preparation method for the medium are provided, wherein the components in the medium formula comprise, by mass, 60% of cottonseed hulls, 25% of corn cob, 5% of corn powder, 6% of wheat bran, 1% of gypsum, 2% of lime, and 1% of calcium superphosphate, a mass ratio of the materials to water is 1:1.2-1.3, and the preparation method comprises: selecting culture materials with no toxic change and no insect biting according to the ratio, treating the culture materials, and carrying out piling fermentation to achieve purposes of the present invention. The preparation method of the present invention has the following advantages that: corn cob and other agricultural wastes are utilized so as to easily achieve agricultural clean production, complete resource utilization and sustainable development; and the prepared culture medium is applicable for high quality rapid growth of pleurotus nebrodensis variety.
Description
Technical field
The invention belongs to the fungus growing technique field, be specially a kind of preparation method of Pleurotus nebrodensis culture substrate.
Background technology
Pleurotus nebrodensis is claimed Pleurotus eryngii var. nebrodensis again, is the white mutation that eryngo is picked up the ears, be again develop in recent years treasure the edible mushrooms new variety; Its mushroom body hypertrophy of Pleurotus nebrodensis is pure white, meat is fine and smooth, mouthfeel good, thick flavor, and nutritious, according to surveying and determination; The Pleurotus nebrodensis protein contnt contains amino acid and reaches 18 kinds up to 14.7%, and lipid content is low; And containing abundant vitamins D and multiple mineral element and trace element, selenium content is rich in fungus polysaccharide isoreactivity material simultaneously again up to 6.8%; The edible long-pending stagnation resolvation, clearing heat and detoxicating that can disappear, be used as medicine then have treatment stomach trouble and typhoid fever, bring high blood pressure down, enhances human body immunological competence and adjusting human body physiological equilibrium.The Methionin that Pleurotus nebrodensis is rich in can make the child increase height, and l-arginine can be improved looks, and L-glutamic acid can make the mushroom flavor more delicious, is a kind of rare environmental protective hygienic article, welcome by consumers in general.
The biological nature of Pleurotus nebrodensis is special, cultivates the DDA that edible fungus species such as bisporous mushroom, flat mushroom, mushroom, auricularia auriculajudae, needle mushroom, white fungus, Coprinus comatus are used with conventional, or comprehensive PDA culture medium, can not turn out good quality strain.DDA cultivates composition: 1000 milliliters of 20% murphy juices, glucose 20 grams, agar 15～20 grams; Combine and to contain PDA substratum composition and be: 1000 milliliters of 20% murphy juices, glucose 20 grams, sal epsom 1.5 grams, di(2-ethylhexyl)phosphate radon potassium 3 grams, 0 milligram of VB11, agar 15～20 grams.Use above-mentioned two kinds of culture medium culturing Pleurotus nebrodensis strains, mycelial growth is very thin, a little less than, climb the wall ability, directly influence output.
The cultivation Pleurotus nebrodensis adopts above-mentioned substrate culture all undesirable, have in addition cause cultivation failure.With cultivation A mode, after culture material inserted Pleurotus nebrodensis strain, mycelia did not grow, and assorted bacterium grows very soon and spreads, the cultivation failure.With cultivation B mode, after inserting Pleurotus nebrodensis strain, the mycelium germination growth, but mycelia a little less than, pollution rate 70%-80% living contaminants appears, after cultivating certain hour.The remainder fruiting is late, and the mushroom body is little, and output is extremely low.
Summary of the invention
The objective of the invention is to biological nature, a kind of culture medium sterilization method that is suitable for the Pleurotus nebrodensis growth is provided, improve edible fungus species culture medium commonly used and cultivating materials sterilising method, improve the benefit of Pleurotus nebrodensis cultivation according to Pleurotus nebrodensis.
To achieve these goals, the present invention adopts following steps:
One, the substrate formula mass ratio that is adopted:
Cotton seed hulls 55～65%,
Corn cob 22～28%,
Semen Maydis powder 3～6%,
Wheat bran 5～8%,
Gypsum 0.5～1.2%,
Lime 1～2.5%,
Superphosphate of lime 0.5～1.5%,
Material, water are than 1: 1.2～1.3.
Two, the preparation method of matrix
1. select not have by formula rate go mouldy, the culture material of free from insect pests, and before using culture material to through being exposed to the sun, pre-treatment such as pulverizing, immersion, then culture material being added water mixes; Fully mix thoroughly; The culture material water cut is reached about 60%, and potential of hydrogen 8～9 is built the heap fermentation then.
2. with the culture material of handling well, pile wide 1.1～1.5m, high 1.2～1.5m, the long strip shape stockpile that length is not limit is got through pore after heap is good, and pitch-row 0.4～0.5m, aperture 6cm make a call to 5 rounds, and 2～3 rows are made a call in the side, heap loam cake plastics film.Ferment, the culture material that ferments should be brown, become thoroughly decomposed evenly, material water cut 60%, potential of hydrogen 7.5～8.5, after can pack cultivation.
The invention has the beneficial effects as follows: the high-quality that 1) is suitable for the Pleurotus nebrodensis kind is grown fast; 2) in the cultivation prescription, utilized agriculture wastes such as corn cob, intended to realize that Agricultural Clean, resource make full use of and Sustainable development; 3) improve the biologicak efficiency of cultivating Pleurotus nebrodensis, realized Pleurotus nebrodensis large-scale planting and high yield and high quality.
Embodiment
Instance 1, combination embodiment elaborate to a kind of preparation method of Pleurotus nebrodensis culture substrate:
(1). the substrate formula mass ratio that is adopted: cotton seed hulls 60%, corn cob 25%, Semen Maydis powder 5%, wheat bran 6%, gypsum 1%, lime 2%, superphosphate of lime 1%, material, water were than 1: 1.2.(2). matrix preparation: press formula rate; The Semen Maydis powder 5% of the cotton seed hulls 60% that the selection nothing is gone mouldy, corn cob 25%, wheat bran 6%, free from insect pests is pulverized cotton seed hulls 60%, corn cob 25% through being exposed to the sun, and then with after gypsum 1%, lime 2%, the superphosphate of lime 1% immersion pre-treatment culture material is added the water mixing; Fully mix thoroughly; Make the culture material water cut reach 60%, potential of hydrogen 8.5 is built the heap fermentation then.(3). the processing of matrix: with the culture material of handling well, pile wide 1.1～1.5m, high 1.2～1.5m, the long strip shape stockpile that length is not limit is got through pore after heap is good, and pitch-row 0.4～0.5m, aperture 6cm make a call to 5 rounds, and 2～3 rows are made a call in the side, heap loam cake plastics film.Ferment, the culture material that ferments should be brown, become thoroughly decomposed evenly, material water cut 60%, potential of hydrogen 7.5～8.5, after can pack cultivation.
The cultivation concrete steps that instance 2, employing prescription of the present invention carry out Pleurotus nebrodensis are following:
(1). select cultivation season, cultivation place.In northern area well-ventilated, clean environment, free of contamination local cultivation, be the mushroom room in the green house of vegetables.In August 20 to September 30 inoculation, November 20 to April in next year fruiting.
(2). the prescription of matrix.Prescription: cotton seed hulls 60%, corn cob 25%, Semen Maydis powder 5%, wheat bran 6%, gypsum 1%, lime 2%, superphosphate of lime 1%, material, water were than 1: 1.2.
(3). the preparation of matrix.By the prescription requirement; The Semen Maydis powder 5% of the cotton seed hulls 60% that the selection nothing is gone mouldy, corn cob 25%, wheat bran 6%, free from insect pests is pulverized cotton seed hulls 60%, corn cob 25% through being exposed to the sun, and then with after gypsum 1%, lime 2%, the superphosphate of lime 1% immersion pre-treatment culture material is added the water mixing; Fully mix thoroughly; Make the culture material water cut reach 60%, potential of hydrogen 8.5 is built the heap fermentation then.
(4). build the heap fermentation.With the culture material of handling well, pile wide 1.4m, high 1.3m, the long strip shape stockpile of long 5m is got through pore after heap is good, and pitch-row 0.4m, aperture 6cm make a call to 5 rounds, and 3 rows are made a call in the side, heap loam cake plastics film.Heap back 60h when the stockpile core temperature reaches 65 ℃, carries out turning first time, ferments, and the culture material that ferments should be brown, becomes thoroughly decomposed evenly, expects water cut 60%, potential of hydrogen 8.5, after can pack.
(5). the pack sterilization.Select wide 20cm, long 43cm, thick 0.04cm high-density low pressure polyethylene plastics bag for use, an end is tightened with cotton rope, and when filling with substance limit rim is pressed with have gentle hands; Elasticity will suit, and tightens sack after installing, in time sterilization; Use normal-pressure sterilization, reach 100 ℃ in the 3h, keep 9h; And then a stewing night, rob the temperature inoculation when treating bag temperature drop to 27 ℃.
(6). inoculation.Connect bacterium chamber the last week what use inoculation; Every cubic metre with 10ml formaldehyde, the sterilization of 5g potassium permanganate; To keep connecing bacterium indoor seasoning, cleaning constantly; After the bacterium bag, inoculating tool, bacterial classification, spirit lamp etc. of bacterium of going out are moved into transfer room, begin inoculation then with the stifling again 30min of aerial fog disinfectant.Connect doorway, bacterium chamber and will place lime powder; The turnover personnel want the sterilization of steppingstone ashes, and the inoculation personnel will wear working suit, band working cap, and working suit and cap will pass through sterilization; Arm will use 150 times of liquid of SANIZOL C to soak 5min, and inoculating tool must be used 75% alcohol wipe and flame sterilization.Seed bottle and bottleneck will be used 0.1% potassium permanganate solution soaking disinfection, and bacterium bag inoculation mouth will can reduce the pollution microbes probability during inoculation in spirit lamp flame aseptic area scope; Inoculum size cuts out the thick aging bacterial classification of strain bag internal skin 2cm earlier by 14% consumption, keeps flat subsequent use; Open sack, in seed bottle, cut out one of broad bean size bacterial classification, put into bag; Tying after during light the pressure; Cross then to move when pocket uses the same method inoculation, tying inoculation and want fast, the tying degree of tightness is wanted appropriateness, is beneficial to ventilate, send out bacterium.
(7). hair tube reason.Send out the bacterium place and advance bag and will clean totally in preceding 10 days, and with the sterilization of aerosol sterilizing case, and sterilized once in per 10 days later on, connect the bacterium bag of bacterial classification, transport a bacterium place after, controlled temperature at 24 ℃, atmospheric moisture 66%.And keeping air condition fresh, half-light to issue bacterium, after 2 weeks, carry out the turning first time, inspection mycelia growing way and living contaminants situation; If when finding to have point-like to mix bacterium, available alcohol is handled, and takes outdoor focusing on when serious; In inoculation 4 weeks of back, mycelial growth is accelerated, and breathes vigorous; This thing will suitably be become flexible tying rope or around sack, prick hole oxygenation with the sterilization toothpick, and notes the room ventilated cooling, grasps the bag temperature at 25 ℃.After 5 weeks, mycelia sends out purseful basically, can carry out the turning second time; The mycelia bag of sending out full He do not send out full is separately put management, and the mycelia bag of sending out full is fruiting immediately not, also will be 16 ℃ of bag temperature; Continue under the condition of relative air humidity 70% to cultivate 60 days; Dense white when mycelia, the bacterium bag is solid, urges the flower bud fruiting when reaching physiological maturity.
(8). management of producing mushroom.Go into the bacterium bag of canopy fruiting, carry out the alternating temperature processing and urge flower bud, temperature to be controlled at 13 ℃, the canopy of can raising evening cooling; Vexed canopy temperature raising on daytime is also given scattered light, after stimulating in 8 days, and the sack charge level mycelia formation fruit body primordium of can twisting together, will keep than constant temperature, humidity this moment; When original hase length is big to silkworm, unclamp sack and dredge flower bud, every bag is stayed 2 healthy and strong mushroom flower buds, and young mushroom is long to expose original hase to the in season sack of walnut size, during the sporophore growth; Mushroom canopy temperature is controlled at 16 ℃, and humidity is controlled at 90%, adopts atomizer spraying and moisturizing earthward, water is not sprayed directly on on the mushroom body; Prevent the flavescence of mushroom body, often ventilation keeps with fresh air in the canopy, gives light scattering.
(9). gather.When the Pleurotus nebrodensis cap fully launches, it is involute that the edge still keeps, and spore not under the bullet, cuts away the root substratum, Dan Duoyong tin foil wrapping vanning as yet.
(10). adopt the quality of the present invention's spirit mushroom bacterium to improve 23%, output has improved 32.6%.
Claims (3)
1. Pleurotus nebrodensis culture substrate, it is characterized in that this matrix prescription comprise: cotton seed hulls 60%, corn cob 25%, Semen Maydis powder 5%, wheat bran 6%, gypsum 1%, lime 2%, superphosphate of lime 1%, material, water are than 1: 1.2～1.3.
2. a kind of according to claim 1 Pleurotus nebrodensis culture substrate; The preparation method who it is characterized in that matrix is: the selection nothing is gone mouldy, the culture material of free from insect pests; And before using culture material to through being exposed to the sun, pre-treatment such as pulverizing, immersion, then culture material being added water mixes, and fully mixes thoroughly; Make the culture material water cut reach 55%～65%, potential of hydrogen 8～9.
3. the preparation method of a kind of Pleurotus nebrodensis culture substrate as claimed in claim 2 is characterized in that: with the culture material of handling well, pile wide 1.1～1.5m, high 1.2～1.5m, the long strip shape stockpile that length is not limit; Get through pore after heap is good, pitch-row 0.4～0.5m, aperture 6cm make a call to 5 rounds, and 2～3 rows are made a call in the side; Heap loam cake plastics film, heap back 48～72h when the stockpile core temperature reaches 65～70 ℃, carries out the turning first time; Ferment, the culture material that ferments should be brown, becomes thoroughly decomposed evenly; The material water cut 60%, potential of hydrogen 7.5～8.5, after can pack.
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Cited By (15)
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| CN103319277A (en) * | 2013-07-18 | 2013-09-25 | 司天戎 | Wild-flavor pleurotus eryngii medium and its preparation method |
| CN103387470A (en) * | 2013-08-08 | 2013-11-13 | 邬金飞 | Pleurotus nebrodensis culture compatible product and preparation method of culture |
| CN103539504A (en) * | 2013-10-24 | 2014-01-29 | 邬方成 | Method for producing pleurotus nebrodensis cultivation material by using waste produced by producing and processing hickory |
| CN103641555A (en) * | 2013-12-06 | 2014-03-19 | 邬金梅 | Method for manufacturing pleurotus nebrodensis culture material from sunflower byproducts |
| CN103880515A (en) * | 2014-04-16 | 2014-06-25 | 山西奥格姆农业科技有限公司 | Cultivation formula of pleurotus nebrodensis |
| CN104177180A (en) * | 2014-08-15 | 2014-12-03 | 陈付峰 | Culture medium capable of improving calcium content of mushroom |
| CN104261942A (en) * | 2014-09-28 | 2015-01-07 | 永和县旺达食用菌有限责任公司 | Pleurotus nebrodensis liquid strain culture medium and propagation method of strain |
| CN104350952A (en) * | 2014-11-25 | 2015-02-18 | 李柳强 | Cultivation method for increasing polysaccharide content of tremella |
| CN104541994A (en) * | 2015-02-06 | 2015-04-29 | 邬方成 | Pleurotus nebrodensis culture method |
| CN104557322A (en) * | 2015-01-28 | 2015-04-29 | 邬金飞 | Production method of pleurotus nebrodensis cultivation material |
| CN106278645A (en) * | 2016-08-22 | 2017-01-04 | 龚恩军 | A kind of organic peat of straw, preparation method and application |
| CN107434690A (en) * | 2016-05-26 | 2017-12-05 | 桂林市粮铭农业科技开发有限公司 | Application of the corncob mulberry tree branch in Pleurotus nebrodensis compost is made |
| CN107434648A (en) * | 2016-05-26 | 2017-12-05 | 黄加丰 | A kind of compost that can improve Pleurotus nebrodensis yield |
| CN108040748A (en) * | 2017-11-29 | 2018-05-18 | 穆世鹏 | A kind of culture materials of edible fungi |
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| CN103319277B (en) * | 2013-07-18 | 2014-10-15 | 司天戎 | Wild-flavor pleurotus eryngii medium and its preparation method |
| CN103319277A (en) * | 2013-07-18 | 2013-09-25 | 司天戎 | Wild-flavor pleurotus eryngii medium and its preparation method |
| CN103387470A (en) * | 2013-08-08 | 2013-11-13 | 邬金飞 | Pleurotus nebrodensis culture compatible product and preparation method of culture |
| CN103387470B (en) * | 2013-08-08 | 2015-03-04 | 邬金飞 | Pleurotus nebrodensis culture compatible product and preparation method of culture |
| CN103539504A (en) * | 2013-10-24 | 2014-01-29 | 邬方成 | Method for producing pleurotus nebrodensis cultivation material by using waste produced by producing and processing hickory |
| CN103539504B (en) * | 2013-10-24 | 2014-12-10 | 邬方成 | Method for producing pleurotus nebrodensis cultivation material by using waste produced by producing and processing hickory |
| CN103641555A (en) * | 2013-12-06 | 2014-03-19 | 邬金梅 | Method for manufacturing pleurotus nebrodensis culture material from sunflower byproducts |
| CN103641555B (en) * | 2013-12-06 | 2015-02-11 | 邬金梅 | Method for manufacturing pleurotus nebrodensis culture material from sunflower byproducts |
| CN103880515A (en) * | 2014-04-16 | 2014-06-25 | 山西奥格姆农业科技有限公司 | Cultivation formula of pleurotus nebrodensis |
| CN104177180B (en) * | 2014-08-15 | 2016-04-06 | 陈付峰 | A kind of cultivation matrix that can improve mushroom calcium contents |
| CN104177180A (en) * | 2014-08-15 | 2014-12-03 | 陈付峰 | Culture medium capable of improving calcium content of mushroom |
| CN104261942A (en) * | 2014-09-28 | 2015-01-07 | 永和县旺达食用菌有限责任公司 | Pleurotus nebrodensis liquid strain culture medium and propagation method of strain |
| CN104350952A (en) * | 2014-11-25 | 2015-02-18 | 李柳强 | Cultivation method for increasing polysaccharide content of tremella |
| CN104350952B (en) * | 2014-11-25 | 2016-09-21 | 上海塞翁福农业发展有限公司 | A kind of cultural method improving tremella polysaccharide content |
| CN104557322A (en) * | 2015-01-28 | 2015-04-29 | 邬金飞 | Production method of pleurotus nebrodensis cultivation material |
| CN104541994A (en) * | 2015-02-06 | 2015-04-29 | 邬方成 | Pleurotus nebrodensis culture method |
| CN107434690A (en) * | 2016-05-26 | 2017-12-05 | 桂林市粮铭农业科技开发有限公司 | Application of the corncob mulberry tree branch in Pleurotus nebrodensis compost is made |
| CN107434648A (en) * | 2016-05-26 | 2017-12-05 | 黄加丰 | A kind of compost that can improve Pleurotus nebrodensis yield |
| CN106278645A (en) * | 2016-08-22 | 2017-01-04 | 龚恩军 | A kind of organic peat of straw, preparation method and application |
| CN108040748A (en) * | 2017-11-29 | 2018-05-18 | 穆世鹏 | A kind of culture materials of edible fungi |
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Application publication date: 20121121 |