CN104350952B - A kind of cultural method improving tremella polysaccharide content - Google Patents
A kind of cultural method improving tremella polysaccharide content Download PDFInfo
- Publication number
- CN104350952B CN104350952B CN201410690514.4A CN201410690514A CN104350952B CN 104350952 B CN104350952 B CN 104350952B CN 201410690514 A CN201410690514 A CN 201410690514A CN 104350952 B CN104350952 B CN 104350952B
- Authority
- CN
- China
- Prior art keywords
- temperature
- parts
- tremella
- grams
- cultivate
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 241001506047 Tremella Species 0.000 title claims abstract description 28
- 150000004676 glycans Chemical class 0.000 title claims abstract description 26
- 229920001282 polysaccharide Polymers 0.000 title claims abstract description 26
- 239000005017 polysaccharide Substances 0.000 title claims abstract description 26
- 238000000034 method Methods 0.000 title claims abstract description 22
- 239000001963 growth medium Substances 0.000 claims abstract description 27
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 claims abstract description 18
- 239000010409 thin film Substances 0.000 claims abstract description 13
- 229910002092 carbon dioxide Inorganic materials 0.000 claims abstract description 9
- 239000001569 carbon dioxide Substances 0.000 claims abstract description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 7
- 241000233866 Fungi Species 0.000 claims description 15
- 229930006000 Sucrose Natural products 0.000 claims description 12
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 12
- 239000011159 matrix material Substances 0.000 claims description 12
- 235000015097 nutrients Nutrition 0.000 claims description 12
- 239000000843 powder Substances 0.000 claims description 12
- 239000005720 sucrose Substances 0.000 claims description 12
- 235000001674 Agaricus brunnescens Nutrition 0.000 claims description 6
- 241000609240 Ambelania acida Species 0.000 claims description 6
- 241000283690 Bos taurus Species 0.000 claims description 6
- 229920000742 Cotton Polymers 0.000 claims description 6
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 6
- 244000068988 Glycine max Species 0.000 claims description 6
- 235000010469 Glycine max Nutrition 0.000 claims description 6
- 239000007836 KH2PO4 Substances 0.000 claims description 6
- 230000001580 bacterial effect Effects 0.000 claims description 6
- 239000010905 bagasse Substances 0.000 claims description 6
- OSGAYBCDTDRGGQ-UHFFFAOYSA-L calcium sulfate Chemical compound [Ca+2].[O-]S([O-])(=O)=O OSGAYBCDTDRGGQ-UHFFFAOYSA-L 0.000 claims description 6
- 210000003608 fece Anatomy 0.000 claims description 6
- OVBPIULPVIDEAO-LBPRGKRZSA-N folic acid Chemical compound C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-LBPRGKRZSA-N 0.000 claims description 6
- 239000008103 glucose Substances 0.000 claims description 6
- 235000008216 herbs Nutrition 0.000 claims description 6
- 239000010871 livestock manure Substances 0.000 claims description 6
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 6
- CSNNHWWHGAXBCP-UHFFFAOYSA-L magnesium sulphate Substances [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 6
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 6
- 210000000582 semen Anatomy 0.000 claims description 6
- 239000002893 slag Substances 0.000 claims description 6
- 239000011727 vitamin B9 Substances 0.000 claims description 6
- 235000015099 wheat brans Nutrition 0.000 claims description 6
- 241000196324 Embryophyta Species 0.000 claims description 3
- 239000000758 substrate Substances 0.000 abstract 1
- 230000001276 controlling effect Effects 0.000 description 7
- 238000009825 accumulation Methods 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 238000002649 immunization Methods 0.000 description 3
- 230000003053 immunization Effects 0.000 description 3
- 239000003795 chemical substances by application Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 1
- 208000036142 Viral infection Diseases 0.000 description 1
- 230000000118 anti-neoplastic effect Effects 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 230000000840 anti-viral effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 230000002596 correlated effect Effects 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000008260 defense mechanism Effects 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 230000002538 fungal effect Effects 0.000 description 1
- 210000000232 gallbladder Anatomy 0.000 description 1
- 229910001385 heavy metal Inorganic materials 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 230000002434 immunopotentiative effect Effects 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 210000000865 mononuclear phagocyte system Anatomy 0.000 description 1
- 239000002547 new drug Substances 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 229910052709 silver Inorganic materials 0.000 description 1
- 239000004332 silver Substances 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 229930193551 sterin Natural products 0.000 description 1
- 238000002560 therapeutic procedure Methods 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/60—Cultivation rooms; Equipment therefor
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
Abstract
The invention discloses a kind of cultural method improving tremella polysaccharide content, including the various measures such as day and night temperature and carbon dioxide control in mother culture media, Cultivar culture medium, substrate selection, the selection of thin film color, rich water quality management, booth, eventually pass through in the Tremella that the present invention processes that polyoses content is the highest adds 16%.
Description
Technical field
The present invention relates to a kind of cultural method, be specifically related to a kind of cultural method improving tremella polysaccharide content.
Background technology
In fungal studies, find that many polysaccharide materials not only have antitumor and higher immunization in recent years,
And there is good pharmacology and clinical utility, and develop into clinical medicine.Modern scientific research is demonstrate,proved
Bright, edible fungi polysaccharide all has potentiation to immunity of organism and cellular immunization；Reticuloendothelial system there is excitement
Function, effect protected to animal livers；There is the antiviral of wide spectrum, antineoplastic immunization and to gallbladder
The dissolution of sterin.At present, Therapeutic Method for the viroses of plant not yet finds suitable medicine in the world
Agent, therefore, searching immunopotentiating agent is one of direction of Developing new drug, and fungus polysaccharide is preferable
Novel immune medicament, its feature is almost without toxicity to host plant, by the defense mechanism of host own
Display curative effect.
Along with the intensification of industrialization degree, while developing the economy, also bring the pollution of environment, particularly
Heavy metal pollution is in aggravation, and edible fungus culturing raw material also have received pollution in various degree, if we are still
If conventionally cultivating, the edible fungi cultivated, also more or less may can contain dirt
Dye material, causes the nutritive value of edible fungi to decline, reduces the health-care effect of edible fungi.
Research for fungus polysaccharide at present has had a part of progress, is mainly concentrated in extracting fungus
The aspect such as polysaccharide and the extracting method how improving polyoses content, and for how improving the cultivation of tremella polysaccharide
The research of aspect is the most rarely known by the people.
Summary of the invention
For the present situation that presently, there are, the technical problem that present invention mainly solves is to provide one can improve silver
The cultural method of fungus polysaccharides, by controlling the day and night temperature of booth, and the amount of carbon dioxide improves polysaccharide
Accumulation, and also improved the content of polysaccharide by suitable cultivation technique.For solving above-mentioned technical problem,
The technical scheme that the present invention uses is:
A kind of cultural method improving tremella polysaccharide content, it is characterised in that use following methods to cultivate:
(1) following mother culture media is selected: Rhizoma Solani tuber osi 200 grams, glucose 20 grams, vitamin B11 gram,
MgSO41.5 grams, KH2PO43 grams, 1000 milliliters of water, pH value natural；
(2) Cultivar culture medium is selected: bagasse 50%, cotton seed hulls 22%, Semen Maydis powder 10%, wheat bran
12%, analysis for soybean powder 4%, sucrose 1%, Gypsum Fibrosum 1%；Tremella bacterial strain is trained in described Cultivar culture medium
Take out after supporting 2～6 days, then under the conditions of temperature is 40～50 DEG C, induce 6～12 minutes；
(3) continue to cultivate: the cultivating white fungus kind culture medium processed through above-mentioned steps (2) is placed in gas bath
In shaking table, 25 DEG C of constant temperature culture, 180 revs/min, cultivate 3～5 days；
(4) set up frame, cover red or purple thin film；
(5) selecting suitable nutrient matrix to cultivate, described nutrient matrix is by the component of following weight portion
Formulated, Caulis et Folium Oryzae 120～200 parts, cattle manure 90～160 parts, medical herbs slag 20～40 parts, Testa Tritici 40～70 parts,
Sucrose 6～12 parts；
(6) controlling temperature of shed, daytime, the plastic house of plantation Tremella, improves temperature of shed, at night
Raising thin film when temperature is minimum and reduce mushroom bed tempertaure, repeatable operation 4～6 days, keeping day and night temperature is 20～30 DEG C,
Keeping canopy humidity 85-90%, in control canopy, the concentration of carbon dioxide is 0.12～0.14%；
(7) timely collecting: when auricle is sufficiently spread out, eight to ninety percent gather time ripe.
In a preferred embodiment of the present invention be: a kind of cultural method improving tremella polysaccharide content, use with
Lower method is cultivated:
(1) following mother culture media is selected: Rhizoma Solani tuber osi 200 grams, glucose 20 grams, vitamin B11 gram,
MgSO41.5 grams, KH2PO43 grams, 1000 milliliters of water, pH value natural；
(2) Cultivar culture medium is selected: bagasse 50%, cotton seed hulls 22%, Semen Maydis powder 10%, wheat bran
12%, analysis for soybean powder 4%, sucrose 1%, Gypsum Fibrosum 1%；By Tremella bacterial strain in described Cultivar culture medium
Take out after cultivating 4 days, then under the conditions of temperature is 45 DEG C, induce 9 minutes；
(3) continue to cultivate: the cultivating white fungus kind culture medium processed through above-mentioned steps (2) is placed in gas bath
In shaking table, 25 DEG C of constant temperature culture, 180 revs/min, cultivate 4 days；
(4) set up frame, cover red or purple thin film；
(5) selecting suitable nutrient matrix to cultivate, described nutrient matrix is by the component of following weight portion
Formulated, Caulis et Folium Oryzae 160 parts, cattle manure 120 parts, medical herbs slag 30 parts, Testa Tritici 55 parts, sucrose 8 parts；
(6) controlling temperature of shed, daytime, the plastic house of plantation Tremella, improves temperature of shed, at night
Raising thin film when temperature is minimum and reduce mushroom bed tempertaure, repeatable operation 5 days, keeping day and night temperature is 25～30 DEG C,
Keeping canopy humidity 85-90%, in control canopy, the concentration of carbon dioxide is 0.13%；
(7) timely collecting: when auricle is sufficiently spread out, about eight to ninety percent gather time ripe.
The invention has the beneficial effects as follows:
The culture medium of the present invention is suitable for the polysaccharide accumulation of Tremella.By controlling the dense of day and night temperature and carbon dioxide
Degree improves the accumulation of polysaccharide, is easier to realize in booth.
The cultural method of the present invention can be effectively improved the polyoses content in Tremella, and it is low to consume energy, and cost increases
Adding few, method is simple, it is easy to control.
Detailed description of the invention
Below presently preferred embodiments of the present invention is described in detail, so that advantages and features of the invention can be more
It is prone to be readily appreciated by one skilled in the art, thus protection scope of the present invention is made apparent clear and definite boundary
Fixed.
Embodiment 1
A kind of cultural method improving tremella polysaccharide content, it is characterised in that use following methods to cultivate:
(1) following mother culture media is selected: Rhizoma Solani tuber osi 200 grams, glucose 20 grams, vitamin B11 gram,
MgSO41.5 grams, KH2PO43 grams, 1000 milliliters of water, pH value natural；
(2) Cultivar culture medium is selected: bagasse 50%, cotton seed hulls 22%, Semen Maydis powder 10%, wheat bran
12%, analysis for soybean powder 4%, sucrose 1%, Gypsum Fibrosum 1%；By Tremella bacterial strain in described Cultivar culture medium
Take out after cultivating 2 days, then under the conditions of temperature is 50 DEG C, induce 6 minutes；
(3) continue to cultivate: the cultivating white fungus kind culture medium processed through above-mentioned steps (2) is placed in gas bath
In shaking table, 25 DEG C of constant temperature culture, 180 revs/min, cultivate 3 days；
(4) set up frame, cover red or purple thin film；
(5) selecting suitable nutrient matrix to cultivate, described nutrient matrix is by the component of following weight portion
Formulated, Caulis et Folium Oryzae 120 parts, cattle manure 90 parts, medical herbs slag 20 parts, Testa Tritici 40 parts, sucrose 6 parts；
(6) controlling temperature of shed, daytime, the plastic house of plantation Tremella, improves temperature of shed, at night
Raising thin film when temperature is minimum and reduce mushroom bed tempertaure, repeatable operation 4 days, keeping day and night temperature is 20～25 DEG C,
Keeping canopy humidity 85-90%, in control canopy, the concentration of carbon dioxide is 0.12%；
(7) timely collecting: when auricle is sufficiently spread out, eight to ninety percent gather time ripe.
After measured, active polysaccharide averagely increases by 12% to the tremella polysaccharide of the present invention.
Embodiment 2
(1) following mother culture media is selected: Rhizoma Solani tuber osi 200 grams, glucose 20 grams, vitamin B11 gram,
MgSO41.5 grams, KH2PO43 grams, 1000 milliliters of water, pH value natural；
(2) Cultivar culture medium is selected: bagasse 50%, cotton seed hulls 22%, Semen Maydis powder 10%, wheat bran
12%, analysis for soybean powder 4%, sucrose 1%, Gypsum Fibrosum 1%；By Tremella bacterial strain in described Cultivar culture medium
Take out after cultivating 4 days, then under the conditions of temperature is 45 DEG C, induce 9 minutes；
(3) continue to cultivate: the cultivating white fungus kind culture medium processed through above-mentioned steps (2) is placed in gas bath
In shaking table, 25 DEG C of constant temperature culture, 180 revs/min, cultivate 4 days；
(4) set up frame, cover red or purple thin film；
(5) selecting suitable nutrient matrix to cultivate, described nutrient matrix is by the component of following weight portion
Formulated, Caulis et Folium Oryzae 160 parts, cattle manure 120 parts, medical herbs slag 30 parts, Testa Tritici 55 parts, sucrose 8 parts；
(6) controlling temperature of shed, daytime, the plastic house of plantation Tremella, improves temperature of shed, at night
Raising thin film when temperature is minimum and reduce mushroom bed tempertaure, repeatable operation 5 days, keeping day and night temperature is 25～30 DEG C,
Keeping canopy humidity 85-90%, in control canopy, the concentration of carbon dioxide is 0.13%；
After measured, active polysaccharide averagely increases by 16% to the tremella polysaccharide of the present invention.
Embodiment 3
A kind of cultural method improving tremella polysaccharide content, it is characterised in that use following methods to cultivate:
(1) following mother culture media is selected: Rhizoma Solani tuber osi 200 grams, glucose 20 grams, vitamin B11 gram,
MgSO41.5 grams, KH2PO43 grams, 1000 milliliters of water, pH value natural；
(2) Cultivar culture medium is selected: bagasse 50%, cotton seed hulls 22%, Semen Maydis powder 10%, wheat bran
12%, analysis for soybean powder 4%, sucrose 1%, Gypsum Fibrosum 1%；By Tremella bacterial strain in described Cultivar culture medium
Take out after cultivating 6 days, then under the conditions of temperature is 40 DEG C, induce 12 minutes；
(3) continue to cultivate: the cultivating white fungus kind culture medium processed through above-mentioned steps (2) is placed in gas bath
In shaking table, 25 DEG C of constant temperature culture, 180 revs/min, cultivate 5 days；
(4) set up frame, cover red or purple thin film；
(5) selecting suitable nutrient matrix to cultivate, described nutrient matrix is by the component of following weight portion
Formulated, Caulis et Folium Oryzae 200 parts, cattle manure 160 parts, medical herbs slag 40 parts, Testa Tritici 70 parts, sucrose 12 parts；
(6) controlling temperature of shed, daytime, the plastic house of plantation Tremella, improves temperature of shed, at night
Raising thin film when temperature is minimum and reduce mushroom bed tempertaure, repeatable operation 6 days, keeping day and night temperature is 25～30 DEG C,
Keeping canopy humidity 85-90%, in control canopy, the concentration of carbon dioxide is 0.14%；
(7) timely collecting: when auricle is sufficiently spread out, eight to ninety percent gather time ripe.
After measured, active polysaccharide averagely increases by 14% to the tremella polysaccharide of the present invention.
The foregoing is only embodiments of the invention, not thereby limit the scope of the claims of the present invention, every profit
The equivalent structure made by description of the invention content or equivalence flow process conversion, or directly or indirectly it is used in it
The technical field that he is correlated with, is the most in like manner included in the scope of patent protection of the present invention.
Claims (1)
1. the cultural method improving tremella polysaccharide content, it is characterised in that use following methods to plant
Training:
(1) following mother culture media is selected: Rhizoma Solani tuber osi 200 grams, glucose 20 grams, vitamin B11 gram,
MgSO41.5 grams, KH2PO43 grams, 1000 milliliters of water, pH value natural；
(2) Cultivar culture medium is selected: bagasse 50%, cotton seed hulls 22%, Semen Maydis powder 10%, wheat bran
12%, analysis for soybean powder 4%, sucrose 1%, Gypsum Fibrosum 1%；Tremella bacterial strain is trained in described Cultivar culture medium
Take out after supporting 2～6 days, then under the conditions of temperature is 40～50 DEG C, induce 6～12 minutes；
(3) continue to cultivate: the cultivating white fungus kind culture medium processed through above-mentioned steps (2) is placed in gas bath
In shaking table, 25 DEG C of constant temperature culture, 180 revs/min, cultivate 3～5 days；
(4) set up frame, cover red or purple thin film；
(5) selecting suitable nutrient matrix to cultivate, described nutrient matrix is by the component of following weight portion
Formulated, Caulis et Folium Oryzae 120～200 parts, cattle manure 90～160 parts, medical herbs slag 20～40 parts, Testa Tritici 40～70 parts,
Sucrose 6～12 parts；
(6) controlling temperature of shed, daytime, the plastic house of plantation Tremella, improves temperature of shed, at night
Raising thin film when temperature is minimum and reduce mushroom bed tempertaure, repeatable operation 4～6 days, keeping day and night temperature is 20～30 DEG C,
Keeping canopy humidity 85-90%, in control canopy, the concentration of carbon dioxide is 0.12～0.14%；
(7) timely collecting: when auricle is sufficiently spread out, eight to ninety percent gather time ripe.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410690514.4A CN104350952B (en) | 2014-11-25 | 2014-11-25 | A kind of cultural method improving tremella polysaccharide content |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410690514.4A CN104350952B (en) | 2014-11-25 | 2014-11-25 | A kind of cultural method improving tremella polysaccharide content |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104350952A CN104350952A (en) | 2015-02-18 |
| CN104350952B true CN104350952B (en) | 2016-09-21 |
Family
ID=52518349
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410690514.4A Active CN104350952B (en) | 2014-11-25 | 2014-11-25 | A kind of cultural method improving tremella polysaccharide content |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104350952B (en) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105315065A (en) * | 2015-05-29 | 2016-02-10 | 姚淑先 | Chinese herbal medicine tremella cultivation technology |
| CN105123268A (en) * | 2015-08-31 | 2015-12-09 | 佛山市高明区更合镇鹏鹄食用菌专业合作社 | High-yield tremella planting method |
| CN106305142A (en) * | 2016-08-23 | 2017-01-11 | 李军 | Method for growing tremella by utilizing fungi grass |
| CN106905064A (en) * | 2017-04-18 | 2017-06-30 | 无限极（中国）有限公司 | A kind of cultivating white fungus culture medium and preparation method thereof, cultivating white fungus method |
| CN107162808B (en) * | 2017-07-14 | 2020-06-02 | 华南农业大学 | Preparation method of culture medium for improving content of tremella polysaccharide |
| CN107493965A (en) * | 2017-08-29 | 2017-12-22 | 贵州普定印象朵贝农业开发有限公司 | A kind of implantation methods for improving white fungus protein content |
| CN112243798B (en) * | 2020-10-22 | 2022-02-22 | 山东同康农业开发有限公司 | Energy-saving and efficient tremella strain breeding and planting method |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1065572A (en) * | 1991-04-11 | 1992-10-28 | 河北省农林科学院农业物理生理生化研究所 | Silvery white edible fungus and its cultural method and culture technique |
| CN1742543A (en) * | 2005-10-29 | 2006-03-08 | 付嵘 | Commercial production method for edible mushroom liquid culture bacteria, apparatus and automatic sterilized inoculator |
| KR100823681B1 (en) * | 2006-02-23 | 2008-04-29 | 중앙대학교 산학협력단 | Composition of culture medium for Tremella fuciformis and culturing method of the same |
| CN102037849A (en) * | 2009-10-12 | 2011-05-04 | 江寿根 | Tremella fuciformis strain culture method |
| CN102786338A (en) * | 2011-05-14 | 2012-11-21 | 郭长瑶 | Preparation method for pleurotus nebrodensis culture medium |
| CN102050673B (en) * | 2010-11-11 | 2013-06-19 | 刘晋才 | Preparation method of edible mushroom wood plant mother culture medium and product thereof |
| CN103493680A (en) * | 2013-09-23 | 2014-01-08 | 重庆市中药研究院 | Tremella liquid cultivar culture method and special culture medium for cultivar |
| CN103960045A (en) * | 2014-05-14 | 2014-08-06 | 谢光玉 | Method for cultivating white funguses through fiber-shaped sugarcane stalks |
-
2014
- 2014-11-25 CN CN201410690514.4A patent/CN104350952B/en active Active
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1065572A (en) * | 1991-04-11 | 1992-10-28 | 河北省农林科学院农业物理生理生化研究所 | Silvery white edible fungus and its cultural method and culture technique |
| CN1742543A (en) * | 2005-10-29 | 2006-03-08 | 付嵘 | Commercial production method for edible mushroom liquid culture bacteria, apparatus and automatic sterilized inoculator |
| KR100823681B1 (en) * | 2006-02-23 | 2008-04-29 | 중앙대학교 산학협력단 | Composition of culture medium for Tremella fuciformis and culturing method of the same |
| CN102037849A (en) * | 2009-10-12 | 2011-05-04 | 江寿根 | Tremella fuciformis strain culture method |
| CN102050673B (en) * | 2010-11-11 | 2013-06-19 | 刘晋才 | Preparation method of edible mushroom wood plant mother culture medium and product thereof |
| CN102786338A (en) * | 2011-05-14 | 2012-11-21 | 郭长瑶 | Preparation method for pleurotus nebrodensis culture medium |
| CN103493680A (en) * | 2013-09-23 | 2014-01-08 | 重庆市中药研究院 | Tremella liquid cultivar culture method and special culture medium for cultivar |
| CN103960045A (en) * | 2014-05-14 | 2014-08-06 | 谢光玉 | Method for cultivating white funguses through fiber-shaped sugarcane stalks |
Non-Patent Citations (1)
| Title |
|---|
| 银耳菌种规范化生产工艺讲座(一);丁湖广;《浙江食用菌》;20100319;第18卷(第2期);第23-25页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104350952A (en) | 2015-02-18 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104350952B (en) | A kind of cultural method improving tremella polysaccharide content | |
| CN102100152B (en) | Artificial culture method and culture medium for fruiting bodies of cordyceps militaris | |
| WO2015196734A1 (en) | An ophiocordyceps sinensis sporocarp artificial cultivation method | |
| CN104221712B (en) | Seafood mushroom and cultural method thereof | |
| CN104285667A (en) | Cultivation method for improving polysaccharide content of grifola frondosa | |
| CN105248150B (en) | A kind of method of cordycepin content in raising fruiting bodies of cordyceps militaris | |
| CN103651151A (en) | Fungus for promoting aquilaria plants to generate agilawood and application of fungus | |
| CN104130068B (en) | A kind of composite multi-functional biological foliage fertilizer | |
| CN107624503B (en) | Shrimp feed additive and application thereof | |
| CN103460994B (en) | Cordyceps militaris nanometer selenium and preparation method thereof | |
| CN103688759A (en) | Method for culturing artificial cordyceps sinensis by using silkworm pupas as carriers | |
| CN103299826A (en) | Cordyceps militaris method capable of improving content of cordycepin | |
| CN108901615A (en) | Utilize the method for trollflower pharmacological property culture medium culture cordyceps mycelium | |
| CN105830972A (en) | Weever fry high survival rate seedling method | |
| KR20120121472A (en) | CULTIVATING METHOD OF MUSHROOM INCLUDING Acanthopanax senticosus HOT WATER EXTRACT | |
| CN107266179A (en) | The fertilizer of the method and its making of culture medium of edible fungus and culturing edible fungus | |
| CN101333550A (en) | Method for preparing CYCLIC DIPEPTIDES compounds and use thereof | |
| CN104885787A (en) | Planting method for cordyceps militaris sporocarp high in active matter content | |
| KR20130081930A (en) | Method for cultivation of cordyceps militaris with saponin or germanium | |
| CN104472208B (en) | Liquid culture method for cordyceps militaris stroma | |
| CN103688744B (en) | A kind of Cordceps militaris breeding method and using method | |
| CN106034738A (en) | Method for manually cultivating silkworm chrysalis cordyceps cicadae by using silkworm chrysalis of domestic silkworms as hosts | |
| CN104285668B (en) | A kind of cultural method improving Flammukinan content | |
| CN106550763B (en) | Artificial culture method of cordyceps sobolifera spore powder | |
| CN104130949B (en) | A kind of the liquid deep layer fermenting culture medium and cultural method of rich iron Cordyceps militaris |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C41 | Transfer of patent application or patent right or utility model | ||
| CB03 | Change of inventor or designer information |
Inventor after: Zhong Minzeng Inventor after: Lin Chengqing Inventor before: Li Liuqiang |
|
| COR | Change of bibliographic data | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20160819 Address after: 200000 Shanghai City Biotechnology Park Fengxian District Economic Development Zone Fengxian Gao Feng Road, No. 666 Applicant after: SHANGHAI SAIWENGFU FOOD CO., LTD. Address before: 545000 the Guangxi Zhuang Autonomous Region Liuzhou City 31 District Yaru road unit 3, room 307 Applicant before: Li Liuqiang |
|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: Cultivation method for increasing polysaccharide content of tremella Effective date of registration: 20170323 Granted publication date: 20160921 Pledgee: Anxin agricultural insurance Limited by Share Ltd Pledgor: SHANGHAI SAIWENGFU FOOD CO., LTD. Registration number: 2017310000010 |
|
| PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
| PC01 | Cancellation of the registration of the contract for pledge of patent right | ||
| PC01 | Cancellation of the registration of the contract for pledge of patent right |
Date of cancellation: 20181214 Granted publication date: 20160921 Pledgee: Anxin agricultural insurance Limited by Share Ltd Pledgor: SHANGHAI SAIWENGFU FOOD CO., LTD. Registration number: 2017310000010 |