CN103688759A - Method for culturing artificial cordyceps sinensis by using silkworm pupas as carriers - Google Patents
Method for culturing artificial cordyceps sinensis by using silkworm pupas as carriers Download PDFInfo
- Publication number
- CN103688759A CN103688759A CN201310728837.3A CN201310728837A CN103688759A CN 103688759 A CN103688759 A CN 103688759A CN 201310728837 A CN201310728837 A CN 201310728837A CN 103688759 A CN103688759 A CN 103688759A
- Authority
- CN
- China
- Prior art keywords
- cordyceps sinensis
- silkworm
- silkworm chrysalis
- inoculation
- pupas
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Mushroom Cultivation (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
A method for culturing artificial cordyceps sinensis by using silkworm pupas as carriers is used for inoculating bacterial suspension prepared by using wild natural cordyceps sinensis bacterial strains native to the Tibet plateau on live silkworm pupas and artificially culturing the live silkworm pupas into silkworm pupa cordyceps sinensis. The method comprises the following steps of (1) screening of cordyceps sinensis bacterial strains, (2) preparation of the silkworm pupas, (3) preparation of bacterial suspension, (4) preparation of silkworm pupas before inoculation, (5) inoculation, (6) culturing of seed seats, and (7) collection and storage of silkworm pupa cordyceps sinensis. According to the method, silkworm pupas of domesticated silkworms serve as hosts, the cordyceps sinensis bacterial strain suspension is inoculated in the silkworm pupas artificially, and then the silkworm pupas are cultured into the silkworm pupa cordyceps sinensis. Inoculation rate and petrification rate after inoculation are above 96%, and the yield of seed entities is above 92%. The period from inoculation to mature of the seed entities takes 36-44 days. The chemical composition of the silkworm pupa cordyceps sinensis is the same with that of cordyceps sinensis basically, and the active ingredients such as cordycepin and cordycepic acids of the silkworm pupa cordyceps sinensis are 3.2-3.9 times the active ingredients of the cordyceps sinensis.
Description
Technical field
The invention belongs to medicinal fungus culture technique field, relate to and a kind ofly take silkworm chrysalis and be that carrier cultivates the method for artificial aweto, relate in particular to wild natural cordyceps bacterial strain that a kind of use originates in Qinghai-Tibet Platean and prepare bacterial suspension inoculation to form the breeding method of artificial aweto on living silkworm chrysalises through artificial culture.
Background technology
Along with the raising of people's living standard with to healthy attention, Cordyceps sinensis and auxiliary product thereof become one and develop industry rapidly.Cordyceps sinensis is classified national second class protection species as, and various places government also appearance policy taboo is dug, limit and dig, but not excavating of Cordyceps sinensis is curbed.Cordyceps sinensis is a kind of rare Chinese medicine of China, by fourth, it needs special growth conditions, vegetative period is longer, thereby very rare in occurring in nature quantity, along with the change of environmental condition, output is downward trend gradually, and social demand is increasing, therefore very famous and precious, price is also high, reach in the market every kilogram of 40,000 yuan of left and right.
Silkworm chrysalis Cordyceps sinensis (Cordyceps militaris) compares with Cordyceps sinensis (Cordyceps): about material during Chinese caterpillar fungus is famous and precious, has very high tonic and be worth and medical value.Kind China Chinese caterpillar fungus has kind more than 200, and what wherein using value was the highest is to originate in the Cordyceps sinensis in Tibet and the Cordyceps militaris that originates in Chang Zishan.The two belongs to Ascomycetes Hypocreales Clavicipitaceae Cordyceps in biological classification.Cordyceps sinensis originates from natural, is that aweto parasitizes bat moth larvae and forms.Silkworm chrysalis Cordyceps sinensis is that cordyceps is inoculated in to silkworm chrysalis body, through artificial culture, forms.The cultivation of silkworm chrysalis Cordyceps sinensis at present has formed scale.Silkworm chrysalis Cordyceps sinensis and Cordyceps sinensis medicine change into minute and pharmacological function all very close.Research shows, in silkworm chrysalis Cordyceps sinensis and Cordyceps sinensis, inorganic elements kind is basically identical, and the content of the two selenium is all higher, yellow luxuriant higher 3.3 times and 5.8 times than selenium-rich anticancer Chinese medicine respectively.Silkworm chrysalis Cordyceps sinensis and Cordyceps sinensis contain the general constituents of identical core, only have cordycepin content silkworm chrysalis Cordyceps sinensis to exceed much than Cordyceps sinensis, and cordycepic acid content silkworm chrysalis Cordyceps sinensis is lower slightly, and the two is suitable for Cordyceps sinensis polysaccharide content.It is basically identical that silkworm chrysalis Cordyceps sinensis and Cordyceps sinensis are approximately imitated function, all there is antitumor, antiviral, antifatigue, androgenic hormone sample, regulate the effects such as lipid metabolism, immune system is had and promoted or dual regulation, can protect the liver, protect liver, resistance of hepatitis B and antibechic, eliminate the phlegm, relieving asthma, spasmolysis, can treat arrhythmia cordis, chronic nephritis and renal failure.
Silkworm chrysalis Cordyceps sinensis and Cordyceps sinensis are all the combinations of insect and fungi, and worm and careless synergy make them have the magical effect of tonifying both YIN and YANG.Cordyceps sinensis wild resource is in great shortage now, and price is constantly soaring, and silkworm chrysalis Cordyceps sinensis can be used as a new health care treasure completely and is widely used.At present; this precious wild resource atrophy of Cordyceps sinensis, resource resources are unclear, regularity of occurrence and development not clear, accelerate research abroad; and silkworm chrysalis Cordyceps sinensis be take silkworm pupa as host; artificial infection cordyceps; simulation Cordyceps militaris natural ecological environment, the success of artificial organic cultivation, is strong protection and the exploitation to Cordyceps sinensis; also be the promotion to the development of silkworm industry, the expansion to plate cocoon industry especially.
At present, domestic having passed through inoculation Cordyceps Militaris by silkworm chrysalis, under the conditions such as certain temperature, humidity, illumination, through more than 40 days take pains to foster, successfully cultivated silkworm chrysalis Cordyceps sinensis.
2005, professor Jiang Xiaolu of Department of Food Engineering of Qingdao Marine University also separation obtained of the same race purebred.Through the chemical analysis analysis comparison to tame Cordyceps militaris and wild cordyceps, result is that various chemical analysis and the content of tame Cordyceps militaris and wild cordyceps is basic identical, and some effective component content is higher than wild cordyceps.
Application number is 93102809.4, and denomination of invention is the patent of " a kind of method of using baked silkworm chrysalis for artificially breeding Chinese caterpillar fungus ", its method be by inoculation on slant medium, carry out the cultivation in mycelia stage; Baking is passed to youngster's silkworm chrysalis rinses well with clear water and dries; The processing of punchinging on using baked silkworm chrysalis; By cleaning the using baked silkworm chrysalis bottling of punchinging, add suitable quantity of water, carry out autoclave sterilization; In inoculating hood, a little mycelia piece of picking is put on the pupal cell of bottle, and envelope bottle is cultivated, and temperature is 15 ℃-30 ℃, and natural lighting, approximately can grow cordyceps militaris sporocarp in two months.Using baked silkworm chrysalis is conducive to a large amount of purchases and long-term storage, and the using baked silkworm chrysalis after punchinging, through autoclave sterilization, can improve the success rate of artificially breeding Chinese caterpillar fungus.And now mostly the silkworm chrysalis of use is that purchase carrys out to obtain the rear silkworm chrysalis of producing of the misty silk of traditional cocoon, contain the chemicalss such as highly basic, not organic silkworm chrysalis, with this, do host and cannot produce organic silkworm chrysalis Cordyceps sinensis.
Application number is 200810013831.7, and denomination of invention is the patent of " a kind of breeding method of silkworm chrysalis Cordyceps sinensis ", and its method is: screening cordyceps bacterial strain; Make the organic medium of silkworm chrysalis; Make spore suspension; Prepare host; Infect cordyceps; Pupal cell is infected in management; Cultivate stroma; Collection and conservation silkworm chrysalis Cordyceps sinensis.The present invention be take silkworm pupa as host, artificial infection cordyceps, and simulation Cordyceps militaris natural ecological environment, artificial culture forms.And the bacterial classification of this invention is spore suspension, the cultivation in later stage is to cultivate under natural environment, and drying and processing adopts high temperature treatment, and this is very large on the impact of the quality of Cordyceps militaris and active ingredient.
Summary of the invention
The object of this invention is to provide the wild natural cordyceps bacterial strain spore inoculating that a kind of use originates in Qinghai-Tibet Platean and to artificial culture on organic living silkworm chrysalises, form the breeding method of silkworm chrysalis Cordyceps sinensis.
For achieving the above object, the breeding method of a kind of silkworm chrysalis Cordyceps sinensis of the present invention, is characterized in that, comprises the following steps:
1) screening of Cordyceps Militaris bacterial strain
The wild fresh Cordyceps strain of Qinghai-Tibet Platean will be originated in, first be inoculated in silkworm chrysalis powder (removing the cured matter of epidermis) as the dull and stereotyped growth of nitrogenous source, treat that mycelium covers with flat board, repeated screening is counted strain until obtain growing way than the pure bacterial strain of Cordyceps sinensis very fast, proterties is stable, usings above-mentioned bacterial strains as object bacterial strain of the present invention;
2) host prepares
Go cocoon shell to obtain live body pupa as host of the present invention the cocoon of domestic tussah, note not destroying the pupa of tussah when removing cocoon;
3) making of bacteria suspension
The Chinese caterpillar fungus strain of screening is inoculated in and is usingd on the culture medium flat plate that silkworm chrysalis powder (remove epidermis cured matter) makes as main nitrogen, put at 20 ℃ and cultivate, after there are a large amount of mycelia, a picking part is inoculated in aforesaid liquid medium and cultivates, cultivate 4-5 days, use high speed discretizer, with 10000r/min, homogenate 1min had both obtained bacteria suspension;
4) before inoculation, silkworm chrysalis is prepared
In aseptic operating platform, live body pupa, with about 75% alcohol disinfecting 6-9 minute, is used to aseptic water washing 5-6 time, dry and do host;
5) inoculation
In aseptic operating platform, with asepsis injector, draw bacteria suspension and inject above-mentioned silkworm chrysalis afterbody or mouthpart, by 0.1-0.15 milliliter/only inject bacteria suspension;
6) cultivation of stroma
Silkworm chrysalis after processing is put in to aseptic vial or large glass dish, protects to pupal cell at 20 ℃ stiff; Then the Sterile culture room of cultivating stiff pupal cell and be placed in the natural lighting that 15-25 ℃, relative air humidity are 85-95% is cultivated;
7) Collection and conservation of silkworm chrysalis Cordyceps sinensis
After stroma maturation, be placed under 60 ℃ of environment and dry 1-5 hour, put shady and cool dry place sealing and preserve, or carry out low tempertaure storage after adopting vacuum cooling drying.
Silkworm chrysalis Cordyceps sinensis of the present invention be take tussah silkworm chrysalis as host, artificial infection bacteria suspension.Success ratio of inoculation, postvaccinal rigid rate reach more than 96%, and the rate that grows of fruit body reaches more than 95%, from being inoculated into the about 35-45 days of maturation of fruit body.The silkworm chrysalis Cordyceps sinensis of the bright artificial culture of property of medicine analytical table on chemical analysis with the difference of Cordyceps sinensis without fundamental character, active ingredient cordycepin and cordycepic acid be Cordyceps sinensis 3.2-3.9 doubly.
Embodiment
Below in conjunction with specific embodiment, the invention will be further described:
Living silkworm chrysalises in the present invention independently provides for our company laboratory.
Silkworm chrysalis Cordyceps sinensis of the present invention is cultivated technical scheme: during artificial culture silkworm chrysalis Cordyceps sinensis, the Chinese caterpillar fungus strain of screening is inoculated in and usings silkworm chrysalis powder (removing the cured matter of epidermis) as on the organic medium of main nitrogen, put at 20 ℃ and cultivate, after there are a large amount of mycelia, a picking part is placed in liquid nutrient medium and makes cultivation, cultivates 4-5 days, smashes mycelium pellet both obtained bacteria suspension by high speed discretizer, by bacterial suspension inoculation in above-mentioned silkworm chrysalis afterbody or mouthpart, to pupal cell until stiff.Then stiff silkworm chrysalis body is placed under the condition that is applicable to Cordyceps militaris cultivation and cultivates.Technology concrete scheme is as follows:
1) screening of Cordyceps Militaris bacterial strain
The wild fresh Cordyceps strain of Qinghai-Tibet Platean will be originated in, first be inoculated in silkworm chrysalis powder (removing the cured matter of epidermis) as the dull and stereotyped growth of nitrogenous source, treat that mycelium covers with flat board, repeated screening is counted strain until obtain growing way than the pure bacterial strain of Cordyceps sinensis very fast, proterties is stable, usings above-mentioned bacterial strains as object bacterial strain of the present invention;
2) host prepares
Go cocoon shell to obtain live body pupa as host of the present invention the cocoon of domestic tussah, note not destroying the pupa of tussah when removing cocoon;
3) making of bacteria suspension
The Chinese caterpillar fungus strain of screening is inoculated in and is usingd on the culture medium flat plate that silkworm chrysalis powder (remove epidermis cured matter) makes as main nitrogen, put at 20 ℃ and cultivate, after there are a large amount of mycelia, a picking part is inoculated in aforesaid liquid medium and cultivates, cultivate 4-5 days, use high speed discretizer, with 10000r/min, homogenate 1min had both obtained bacteria suspension;
4) before inoculation, silkworm chrysalis is prepared
In aseptic operating platform, live body pupa, with about 75% alcohol disinfecting 6-9 minute, is used to aseptic water washing 5-6 time, dry and do host;
5) inoculation
In aseptic operating platform, with asepsis injector, draw bacteria suspension and inject above-mentioned silkworm chrysalis afterbody or mouthpart, by 0.1-0.15 milliliter/only inject bacteria suspension;
6) cultivation of stroma
Silkworm chrysalis after processing is put in to aseptic vial or large glass dish, protects to pupal cell at 20 ℃ stiff; Then the Sterile culture room of cultivating stiff pupal cell and be placed in the natural lighting that 15-25 ℃, relative air humidity are 85-95% is cultivated;
7) Collection and conservation of silkworm chrysalis Cordyceps sinensis
After stroma maturation, be placed under 60 ℃ of environment and dry 1-5 hour, put shady and cool dry place sealing and preserve, or carry out low tempertaure storage after adopting vacuum cooling drying.
Embodiment mono-:
The artificial aweto breeding method that the living silkworm chrysalises of take is medium.
Concrete technical scheme is as follows:
(1) screening of Cordyceps Militaris bacterial strain
1, bacterial classification is made
Choose high yield, high-quality, precocity, originate in Qinghai-Tibet wild fresh cordyceps sporophore, alcohol with 75% carries out surface sterilization, with sterile water wash several (general 5-6 time), the polypide in Cordyceps sinensis is removed, remainder is positioned in medium and cultivates.Repurity after mycelia is covered with flat board.Through repeatedly obtaining the female kind of number strain after purifying.
After mother is planted and expand cultivated, be placed in and using silkworm chrysalis powder (removing the cured matter of epidermis) and, as on the organic medium of nitrogenous source, at 18 ℃-25 ℃, cultivate 20-30d, observe growing state, have living contaminants if see, the female kind of reply is further purified; If without living contaminants, can continue to cultivate, after one month, there is orange red fruitbody production, female kind is reliably described.
Liquid nutrient medium is sub-packed in 500ml triangular flask, and 121 ℃, sterilizing 20 minutes, plants mother with oese, to receive in triangular flask under aseptic condition.Inoculation is placed under 18-22 ℃ of condition, shakes kind of a cultivation 6d left and right, after even bead to be formed, with high speed disperser high speed 10000r/min, processes 1min and has both obtained bacteria suspension.
2, host prepares
Go cocoon shell to obtain live body pupa as host of the present invention the cocoon of domestic tussah, note not destroying the pupa of tussah when removing cocoon.In aseptic operating platform, live body pupa, with about 75% alcohol disinfecting 6-9 minute, is used to aseptic water washing 1 minute, dry and do host;
3, inoculation
In aseptic operating platform, with asepsis injector, draw bacteria suspension and inject above-mentioned silkworm chrysalis afterbody or mouthpart, by 0.1-0.15 milliliter/only inject bacteria suspension;
4, the cultivation of stroma
Silkworm chrysalis after processing is put in to aseptic vial or large glass dish, protects to pupal cell at 20 ℃ stiff; Then the Sterile culture room of cultivating stiff pupal cell and be placed in the natural lighting that 15-25 ℃, relative air humidity are 85-95% is cultivated;
5, the Collection and conservation of silkworm chrysalis Cordyceps sinensis
After stroma maturation, be placed under 60 ℃ of environment and dry 1-5 hour, put shady and cool dry place sealing and preserve, or in order to prevent that high temperature from destroying the part nutrients in Chinese caterpillar fungus, can adopt after vacuum cooling drying low tempertaure storage.
Claims (1)
1. the method that the silkworm chrysalis of take is cultivated artificial aweto as carrier, is characterized in that, comprises the following steps:
1) screening of Cordyceps Militaris bacterial strain
The wild fresh Cordyceps strain of Qinghai-Tibet Platean will be originated in, first be inoculated in silkworm chrysalis powder (removing the cured matter of epidermis) as the dull and stereotyped growth of nitrogenous source, treat that mycelium covers with flat board, repeated screening is counted strain until obtain growing way than the pure bacterial strain of Cordyceps sinensis very fast, proterties is stable, usings above-mentioned bacterial strains as object bacterial strain of the present invention;
2) host prepares
Go cocoon shell to obtain live body pupa as host of the present invention the cocoon of domestic tussah, note not destroying the pupa of tussah when removing cocoon;
3) making of bacteria suspension
The Chinese caterpillar fungus strain of screening is inoculated in and is usingd on the culture medium flat plate that silkworm chrysalis powder (remove epidermis cured matter) makes as main nitrogen, put at 20 ℃ and cultivate, after there are a large amount of mycelia, a picking part is inoculated in aforesaid liquid medium and cultivates, cultivate 4-5 days, use high speed discretizer, with 10000r/min, homogenate 1min had both obtained bacteria suspension;
4) before inoculation, silkworm chrysalis is prepared
In aseptic operating platform, live body pupa, with about 75% alcohol disinfecting 6-9 minute, is used to aseptic water washing 5-6 time, dry and do host;
5) inoculation
In aseptic operating platform, with asepsis injector, draw bacteria suspension and inject above-mentioned silkworm chrysalis afterbody or mouthpart, by 0.1-0.15 milliliter/only inject bacterium liquid;
6) cultivation of stroma
Silkworm chrysalis after processing is put in to aseptic vial or large glass dish, protects to pupal cell at 20 ℃ stiff; Then the Sterile culture room of cultivating stiff pupal cell and be placed in the natural lighting that 15-25 ℃, relative air humidity are 85-95% is cultivated;
7) Collection and conservation of silkworm chrysalis Cordyceps sinensis
After stroma maturation, be placed under 60 ℃ of environment and dry 1-5 hour, put shady and cool dry place sealing and preserve, or carry out low tempertaure storage after adopting vacuum cooling drying.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310728837.3A CN103688759A (en) | 2013-12-20 | 2013-12-20 | Method for culturing artificial cordyceps sinensis by using silkworm pupas as carriers |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310728837.3A CN103688759A (en) | 2013-12-20 | 2013-12-20 | Method for culturing artificial cordyceps sinensis by using silkworm pupas as carriers |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103688759A true CN103688759A (en) | 2014-04-02 |
Family
ID=50350552
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310728837.3A Pending CN103688759A (en) | 2013-12-20 | 2013-12-20 | Method for culturing artificial cordyceps sinensis by using silkworm pupas as carriers |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103688759A (en) |
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103704022A (en) * | 2013-12-20 | 2014-04-09 | 凌中鑫 | Method for cultivating artificial cordyceps sinensis with five-instar silkworms as carrier |
| CN103960055A (en) * | 2014-05-15 | 2014-08-06 | 高青和润丝绸有限公司 | Method for cultivating silkworm cordyceps |
| CN104303835A (en) * | 2014-09-29 | 2015-01-28 | 苏州家和农业生物科技研究中心 | Cultivation method of cordyceps sinensis |
| CN104429596A (en) * | 2014-11-13 | 2015-03-25 | 北京天地同源生物科技有限公司 | Method for producing silkworm insect grass by injecting cordyceps militaris to matured silkworms |
| CN104904493A (en) * | 2015-04-08 | 2015-09-16 | 丹东中天柞蚕生物科技有限公司 | Method for artificially cultivating Chinese-oak-silkworm-pupa cordyceps militaris through yellow Chinese oak silkworm pupae |
| CN105875180A (en) * | 2014-05-14 | 2016-08-24 | 四川久远生物技术有限公司 | Silkworm chrysalis two-step refrigeration line production Chinese caterpillar fungus cultivation method |
| CN104429596B (en) * | 2014-11-13 | 2017-01-04 | 北京天地同源生物科技有限公司 | The method that matured silkworm injection Cordyceps militaris (L.) Link. fungus produces silkworm grass |
| CN108370816A (en) * | 2016-10-12 | 2018-08-07 | 镇江市天益生物科技有限公司 | A kind of production method of rejuvenation Cordyceps militaris spawn |
| CN109952912A (en) * | 2019-03-08 | 2019-07-02 | 广东省生物资源应用研究所 | A kind of method that Hirsutella sinensis infection host insect bat moth larvae obtains cordyceps sinensis |
| CN110754662A (en) * | 2019-11-06 | 2020-02-07 | 广东省农业科学院蚕业与农产品加工研究所 | Yellow blood silkworm cordyceps militaris chicken essence electuary suitable for people prone to fatigue and preparation method thereof |
| CN112190602A (en) * | 2020-10-27 | 2021-01-08 | 苏州家和蚕业生物科技有限公司 | Preparation method of silkworm chrysalis cordyceps sinensis tablets |
| CN114793755A (en) * | 2022-04-18 | 2022-07-29 | 常熟理工学院 | Method for cultivating silkworm cordyceps militaris with high rigidification rate |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1034390A (en) * | 1987-10-13 | 1989-08-02 | 吉林省蚕业科学研究所 | The artificial culturing method of silkworm chrysalis Cordyceps sinensis |
| CN1861782A (en) * | 2006-05-26 | 2006-11-15 | 李宏 | Artificial cultivating method of enriched selenium Cordyceps militaris and application of fruiting body |
| CN101182471A (en) * | 2007-11-16 | 2008-05-21 | 江苏科技大学 | Highly effective cultivating method for Chinese caterpillar fungus |
| CN101215527A (en) * | 2008-01-11 | 2008-07-09 | 丁啸南 | Method for cultivating silkworm chrysalis Cordyceps sinensis |
| CN102308722A (en) * | 2010-07-09 | 2012-01-11 | 王润蛟 | Factory production technology for high-cordycepin cordyceps militaris |
| CN103430776A (en) * | 2013-09-04 | 2013-12-11 | 鲁东大学 | Silkworm pupa-worm grass culture method capable of improving yield and quality |
-
2013
- 2013-12-20 CN CN201310728837.3A patent/CN103688759A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1034390A (en) * | 1987-10-13 | 1989-08-02 | 吉林省蚕业科学研究所 | The artificial culturing method of silkworm chrysalis Cordyceps sinensis |
| CN1861782A (en) * | 2006-05-26 | 2006-11-15 | 李宏 | Artificial cultivating method of enriched selenium Cordyceps militaris and application of fruiting body |
| CN101182471A (en) * | 2007-11-16 | 2008-05-21 | 江苏科技大学 | Highly effective cultivating method for Chinese caterpillar fungus |
| CN101215527A (en) * | 2008-01-11 | 2008-07-09 | 丁啸南 | Method for cultivating silkworm chrysalis Cordyceps sinensis |
| CN102308722A (en) * | 2010-07-09 | 2012-01-11 | 王润蛟 | Factory production technology for high-cordycepin cordyceps militaris |
| CN103430776A (en) * | 2013-09-04 | 2013-12-11 | 鲁东大学 | Silkworm pupa-worm grass culture method capable of improving yield and quality |
Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103704022A (en) * | 2013-12-20 | 2014-04-09 | 凌中鑫 | Method for cultivating artificial cordyceps sinensis with five-instar silkworms as carrier |
| CN105875180A (en) * | 2014-05-14 | 2016-08-24 | 四川久远生物技术有限公司 | Silkworm chrysalis two-step refrigeration line production Chinese caterpillar fungus cultivation method |
| CN103960055A (en) * | 2014-05-15 | 2014-08-06 | 高青和润丝绸有限公司 | Method for cultivating silkworm cordyceps |
| CN104303835A (en) * | 2014-09-29 | 2015-01-28 | 苏州家和农业生物科技研究中心 | Cultivation method of cordyceps sinensis |
| CN104429596B (en) * | 2014-11-13 | 2017-01-04 | 北京天地同源生物科技有限公司 | The method that matured silkworm injection Cordyceps militaris (L.) Link. fungus produces silkworm grass |
| CN104429596A (en) * | 2014-11-13 | 2015-03-25 | 北京天地同源生物科技有限公司 | Method for producing silkworm insect grass by injecting cordyceps militaris to matured silkworms |
| CN104904493A (en) * | 2015-04-08 | 2015-09-16 | 丹东中天柞蚕生物科技有限公司 | Method for artificially cultivating Chinese-oak-silkworm-pupa cordyceps militaris through yellow Chinese oak silkworm pupae |
| CN108370816A (en) * | 2016-10-12 | 2018-08-07 | 镇江市天益生物科技有限公司 | A kind of production method of rejuvenation Cordyceps militaris spawn |
| CN109952912A (en) * | 2019-03-08 | 2019-07-02 | 广东省生物资源应用研究所 | A kind of method that Hirsutella sinensis infection host insect bat moth larvae obtains cordyceps sinensis |
| CN109952912B (en) * | 2019-03-08 | 2021-06-15 | 广东省科学院动物研究所 | Method for obtaining cordyceps sinensis by infecting host insect hepialus larva with hirsutella sinensis |
| CN110754662A (en) * | 2019-11-06 | 2020-02-07 | 广东省农业科学院蚕业与农产品加工研究所 | Yellow blood silkworm cordyceps militaris chicken essence electuary suitable for people prone to fatigue and preparation method thereof |
| CN110754662B (en) * | 2019-11-06 | 2022-09-06 | 广东省农业科学院蚕业与农产品加工研究所 | Yellow blood silkworm cordyceps militaris chicken essence electuary suitable for people prone to fatigue and preparation method thereof |
| CN112190602A (en) * | 2020-10-27 | 2021-01-08 | 苏州家和蚕业生物科技有限公司 | Preparation method of silkworm chrysalis cordyceps sinensis tablets |
| CN114793755A (en) * | 2022-04-18 | 2022-07-29 | 常熟理工学院 | Method for cultivating silkworm cordyceps militaris with high rigidification rate |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103688759A (en) | Method for culturing artificial cordyceps sinensis by using silkworm pupas as carriers | |
| CN103430776B (en) | Silkworm pupa-worm grass culture method capable of improving yield and quality | |
| JP6143245B2 (en) | Artificial culture method of Cordyceps fruit body | |
| CN102100152B (en) | Artificial culture method and culture medium for fruiting bodies of cordyceps militaris | |
| CN102138437B (en) | Artificial cultivation method for Taiwan cordyceps fruiting bodies | |
| CN103651151B (en) | Fungus for promoting aquilaria plants to generate agilawood and application of fungus | |
| CN101215527A (en) | Method for cultivating silkworm chrysalis Cordyceps sinensis | |
| CN103650914B (en) | A kind of soilless culture method of Aode mushroom | |
| CN103563655A (en) | Technique for cultivating living pupation cordyceps | |
| CN103918472B (en) | The cultivation basswood method of Antrodia Camphorata | |
| CN104541977B (en) | A kind of Chinese fir board culture method of Antrodia camphorata | |
| CN101045904A (en) | Aweto sporophore culturing process | |
| CN103858676A (en) | Method for cultivating liquid cultures of cordyceps militaris | |
| CN104845892A (en) | R.vinctus and application thereof in promoting aquilaria plants to produce agilawood | |
| CN103704022A (en) | Method for cultivating artificial cordyceps sinensis with five-instar silkworms as carrier | |
| CN103688760A (en) | Method for culturing artificial cordyceps sinensis by using yellow mealworms as carriers | |
| CN103563649A (en) | Cultivation method of cordyceps militaris | |
| CN105557312A (en) | Method for cultivating Cordyceps products by taking Tenebrio molitor (L.) larvae as carriers | |
| CN113330984A (en) | Artificial cultivation method for improving cordyceps sobolifera polysaccharide content in cordyceps sobolifera sporocarp | |
| CN104982225A (en) | Novel male silkworm cordyceps culturing process and method for culturing silkworm cordyceps parent species | |
| CN103749149A (en) | Method for cultivating artificial worm grass with mealworm pupa as carrier | |
| CN104756759B (en) | A kind of Rhizoma Dioscoreae Cordyceps and cultural method thereof | |
| CN103270890A (en) | Method of culturing Taiwanofungus camphoratus by dish | |
| CN105493899A (en) | Method for cultivating cordyceps sinensis products with mealworm pupae as carriers | |
| CN105230482A (en) | Method for establishing in-vitro regeneration system of Acrostichum aureurm |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20140402 |