CN103430776B - Silkworm pupa-worm grass culture method capable of improving yield and quality - Google Patents
Silkworm pupa-worm grass culture method capable of improving yield and quality Download PDFInfo
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Abstract
The invention discloses a silkworm pupa-worm grass culture method capable of improving yield and quality. The method is characterized by comprising the steps including preparation of liquid spawn, pupa sterilization, inoculation, cultivation in dark, wound treatment of pupas, cultivation in light, harvest and the like. Compared with the conventional culture method, the silkworm pupa-worm grass culture method disclosed by the invention is short in grass germination time, great in fruit body number, vigorous in growth and short in growth period, so that not only can the yield of the worm grass be increased, but also the growth period can be greatly shortened, and thus the economic benefits are greatly increased.
Description
Technical field
The present invention relates to a kind of method that improves silkworm chrysalis Cordyceps sinensis output and quality.
Background technology
The cultural method of traditional silkworm chrysalis Cordyceps sinensis, incubation time is long, generally needs 60-80 days, and stroma quantity is few, is generally 3-5 bar, and its output and quality all need further to be improved.
Summary of the invention
The object of the present invention is to provide a kind of method is easy, incubation time is short, output is high raising output and the silkworm chrysalis Cordyceps sinensis cultural method of quality.
Technical solution of the present invention is:
A silkworm chrysalis Cordyceps sinensis cultural method that improves output and quality, is characterized in that: comprise the following steps:
(1) the preparation of liquid spawn:
Liquid nutrient medium preparation: the component of following weight portion is mixed and made medium: 10 ~ 30 parts of glucose, potassium dihydrogen phosphate 1-5 part, magnesium sulfate 1-5 part, 5 ~ 10 parts of peptones, yeast soaks powder 2-5 part, vitaminB10 .1 ~ 0.5 part, 1000 parts, water;
Bacterial classification preparation: the liquid nutrient medium preparing is sub-packed in conical flask, 200 ~ 250 milliliters every bottle, sealing, under 120 ℃ ~ 130 ℃ conditions, sterilizing is 18 ~ 30 minutes; After sterilizing, take out medium, naturally cool to room temperature, under aseptic condition, access Cordyceps militaris spawn; At 15 ~ 30 ℃, in shaking table, 5-10 days is cultivated in concussion;
(2) pupal cell sterilization:
Get fresh and alive tussah chrysalis, with alcohol-pickled 1-3 minute, take out and be put into ultraviolet sterilization 15-30 minute on superclean bench;
(3) inoculation:
Liquid spawn is injected in pupal cell to each pupal cell 0.1-0.5 milliliter bacterial classification with syringe;
(4) dark culturing:
The pupal cell of inoculation is divided in bottle, each bottle 3-5, be placed in constant incubator, cultivate 5-10 days for 18-25 ℃, after it is stiff, take out; Under natural daylight, 18-25 ℃, cultivates 5-10 days under air humidity 50%-65% condition;
(5) pupal cell wound is processed:
At the pupal cell outside of belly, with scissors, cut long 2-3 centimetre, the openning 2-4 bar of dark 2-4 millimeters long, then each pupal cell outside of belly is placed in blake bottle upward;
(6) light is cultivated:
Under natural daylight, temperature 18-25 ℃, continues to cultivate 25-30 days under air humidity 65%-85% condition;
(7) gather
When light is cultured to fruit body and grows to 5-8 centimeter length, gather.
When in step (1) prepared by bacterial classification, shaking speed is 120 revs/min.
Step is pupal cell when sterilization (2), and the mass concentration of alcohol is 75%.
The present invention is when silkworm chrysalis ossifys latter 5-10 days, silkworm chrysalis is carried out to wound processing, the mycelia that makes to be enclosed in silkworm chrysalis body touches air and light, processing not only can be shortened going out the grass time of silkworm chrysalis Cordyceps sinensis greatly like this, also can increase grass output amount simultaneously, make fruit body look more slightly more strong, maturation time is shortened greatly.From being inoculated into stroma maturation, by normal 60-80 days, shorten to 40-50 days, more than stroma quantity is increased to 15-25 bar by 3-5 bar, length can reach 7-10 centimetre, fruit-body color after oven dry is bright-coloured, neatly, quality is high, and output improves more than 25% than common cultural method.With traditional cultural method relatively this cultural method to have the grass time short, fruit body quantity is many, grows vigorous, growth cycle shortens, and not only can improve the output of Chinese caterpillar fungus, has also greatly shortened growth cycle simultaneously, and economic benefit is improved significantly.
Below in conjunction with embodiment, the invention will be further described.
Embodiment
Embodiment 1:
A silkworm chrysalis Cordyceps sinensis cultural method that improves output and quality, comprises the following steps:
(1) the preparation of liquid spawn:
Liquid nutrient medium preparation: the component of following weight portion is mixed and made medium: 20 grams of glucose, 3 grams of potassium dihydrogen phosphates, 4 grams, magnesium sulfate, 10 grams of peptones, 0.3 gram of vitamin B1, yeast soaks 2 grams, powder, water 1000ml;
Bacterial classification preparation: the liquid nutrient medium preparing is sub-packed in conical flask, 220 milliliters every bottle, sealing, under 130 ℃ of conditions, sterilizing is 18 minutes; After sterilizing, take out medium, naturally cool to room temperature, under aseptic condition, access Cordyceps militaris spawn; At 20 ℃, in the shaking table of 120 revs/min, concussion is cultivated 7 days;
(2) pupal cell sterilization:
Get tussah chrysalis, with 75% alcohol-pickled 1 minute, take out and be put on superclean bench ultraviolet sterilization 22 minutes;
(3) inoculation:
Liquid spawn is injected in pupal cell to 0.5 milliliter of bacterial classification of each pupal cell with syringe;
(4) dark culturing:
The pupal cell of inoculation is divided in bottle, and 3, each bottle, is placed in constant incubator, cultivates 10 days for 20 ℃, after it is stiff, takes out; Under natural daylight, 22 ℃, under air humidity 60% condition, cultivate 8 days;
(5) pupal cell wound is processed:
At the pupal cell outside of belly, with scissors, cut long 2 centimetres, 4, the openning of dark 3 millimeters long, then each pupal cell outside of belly is placed in blake bottle upward;
(6) light is cultivated:
Under natural daylight, 22 ℃ of temperature, continue under air humidity 75% condition to cultivate 28 days;
(7) gather
When light is cultured to fruit body and grows to 5-8 centimeter length, gather.
Embodiment 2:
A silkworm chrysalis Cordyceps sinensis cultural method that improves output and quality, comprises the following steps:
(1) the preparation of liquid spawn:
Liquid nutrient medium preparation: the component of following weight portion is mixed and made medium: 25 grams of glucose, 5 grams of potassium dihydrogen phosphates, 1 gram, magnesium sulfate, 5 grams of peptones, 0.5 gram of vitamin B1, yeast soaks 5 grams, powder, water 1000ml;
Bacterial classification preparation: the liquid nutrient medium preparing is sub-packed in conical flask, 200 milliliters every bottle, sealing, under 125 ℃ of conditions, sterilizing is 25 minutes; After sterilizing, take out medium, naturally cool to room temperature, under aseptic condition, access Cordyceps militaris spawn; At 25 ℃, in the shaking table of 120 revs/min, concussion is cultivated 5 days;
(2) pupal cell sterilization:
Get tussah chrysalis, with 75% alcohol-pickled 2 minutes, take out and be put on superclean bench ultraviolet sterilization 30 minutes;
(3) inoculation:
Liquid spawn is injected in pupal cell to 0.3 milliliter of bacterial classification of each pupal cell with syringe;
(4) dark culturing:
The pupal cell of inoculation is divided in bottle, and 4, each bottle, is placed in constant incubator, cultivates 8 days for 25 ℃, after it is stiff, takes out; Under natural daylight, 25 ℃, under air humidity 65% condition, cultivate 10 days;
(5) pupal cell wound is processed:
At the pupal cell outside of belly, with scissors, cut long 3 centimetres, 2, the openning of dark 4 millimeters long, then each pupal cell outside of belly is placed in blake bottle upward;
(6) light is cultivated:
Under natural daylight, 20 ℃ of temperature, continue under air humidity 67% condition to cultivate 30 days;
(7) gather
When light is cultured to fruit body and grows to 5-8 centimeter length, gather.
Embodiment 3:
A silkworm chrysalis Cordyceps sinensis cultural method that improves output and quality, comprises the following steps:
(1) the preparation of liquid spawn:
Liquid nutrient medium preparation: the component of following weight portion is mixed and made medium: 30 grams of glucose, 1 gram of potassium dihydrogen phosphate, 3 grams, magnesium sulfate, 6 grams of peptones, vitaminB10 .1 gram, yeast soaks 3 grams, powder, water 1000ml;
Bacterial classification preparation: the liquid nutrient medium preparing is sub-packed in conical flask, 230 milliliters every bottle, sealing, under 120 ℃ of conditions, sterilizing is 26 minutes; After sterilizing, take out medium, naturally cool to room temperature, under aseptic condition, access Cordyceps militaris spawn; At 26 ℃, in the shaking table of 120 revs/min, concussion is cultivated 5 days;
(2) pupal cell sterilization:
Get tussah chrysalis, with 75% alcohol-pickled 2 minutes, take out and be put on superclean bench ultraviolet sterilization 30 minutes;
(3) inoculation:
Liquid spawn is injected in pupal cell to 0.3 milliliter of bacterial classification of each pupal cell with syringe;
(4) dark culturing:
The pupal cell of inoculation is divided in bottle, and 5, each bottle, is placed in constant incubator, cultivates 7 days for 18 ℃, after it is stiff, takes out; Under natural daylight, 25 ℃, under air humidity 65% condition, cultivate 9 days;
(5) pupal cell wound is processed:
At the pupal cell outside of belly, with scissors, cut long 2.5 centimetres, 3, the openning of dark 3 millimeters long, then each pupal cell outside of belly is placed in blake bottle upward;
(6) light is cultivated:
Under natural daylight, 23 ℃ of temperature, continue under air humidity 68% condition to cultivate 30 days;
(7) gather
When light is cultured to fruit body and grows to 5-8 centimeter length, gather.
Claims (3)
1. a silkworm chrysalis Cordyceps sinensis cultural method that improves output and quality, is characterized in that: comprise the following steps:
(1) the preparation of liquid spawn:
Liquid nutrient medium preparation: the component of following weight portion is mixed and made medium: 10 ~ 30 parts of glucose, 1 ~ 5 part of potassium dihydrogen phosphate, 1 ~ 5 part, magnesium sulfate, 5 ~ 10 parts of peptones, yeast soaks powder 2-5 part, vitaminB10 .1 ~ 0.5 part, 1000 parts, water;
Bacterial classification preparation: the liquid nutrient medium preparing is sub-packed in conical flask, 200 ~ 250 milliliters every bottle, sealing, under 120 ℃ ~ 130 ℃ conditions, sterilizing is 18 ~ 30 minutes; After sterilizing, take out medium, naturally cool to room temperature, under aseptic condition, access Cordyceps militaris spawn; At 15 ~ 30 ℃, in shaking table, 5-10 days is cultivated in concussion;
(2) pupal cell sterilization:
Get fresh and alive tussah chrysalis, with alcohol-pickled 1-3 minute, take out and be put into ultraviolet sterilization 15-30 minute on superclean bench;
(3) inoculation:
Liquid spawn is injected in pupal cell to each pupal cell 0.1-0.5 milliliter bacterial classification with syringe;
(4) dark culturing:
The pupal cell of inoculation is divided in bottle, each bottle 3-5, sealing, is placed in constant incubator, cultivates 5-10 days at 18-25 ℃, after it is stiff, takes out; Under natural daylight, 18-25 ℃, cultivates 5-10 days under air humidity 50%-65% condition;
(5) pupal cell wound is processed:
At the pupal cell outside of belly, with scissors, cut long 2-3 centimetre, the openning 2-4 bar of dark 2-4 millimeters long, then each pupal cell outside of belly is placed in blake bottle upward;
(6) light is cultivated:
Under natural daylight, temperature 18-25 ℃, continues to cultivate 25-30 days under air humidity 65%-85% condition;
(7) gather
When light is cultured to fruit body and grows to 5-8 centimeter length, gather.
2. the silkworm chrysalis Cordyceps sinensis cultural method of raising output according to claim 1 and quality, is characterized in that: when in step (1) prepared by bacterial classification, shaking speed is 120 revs/min.
3. the silkworm chrysalis Cordyceps sinensis cultural method of raising output according to claim 1 and quality, is characterized in that: step is (2) during pupal cell sterilization, and the mass concentration of alcohol is 75%.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108812050A (en) * | 2018-05-11 | 2018-11-16 | 江苏圣福来生态农业有限公司 | A kind of culture implantation methods improving Cordyceps militaris yield |
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| CN101849477A (en) * | 2009-03-30 | 2010-10-06 | 张天宝 | Cassava silkworm coydyceps militaris (L.) link cultivated by taking cassava silkworm chrysalis as host and cultivating method thereof |
| CN102342216A (en) * | 2010-07-29 | 2012-02-08 | 于兴军 | Method by combining physical method and chemical method to improve quality of cordyceps militaris |
| CN102100152B (en) * | 2010-11-17 | 2012-05-23 | 鲁东大学 | Artificial culture method and culture medium for fruiting bodies of cordyceps militaris |
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| CN108812050A (en) * | 2018-05-11 | 2018-11-16 | 江苏圣福来生态农业有限公司 | A kind of culture implantation methods improving Cordyceps militaris yield |
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Denomination of invention: Culture method of Cordyceps militaris for improving yield and quality Effective date of registration: 20211216 Granted publication date: 20141210 Pledgee: Yantai financing guarantee Group Co.,Ltd. Pledgor: LUDONG University Registration number: Y2021980015152 |
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Date of cancellation: 20220317 Granted publication date: 20141210 Pledgee: Yantai financing guarantee Group Co.,Ltd. Pledgor: LUDONG University Registration number: Y2021980015152 |