CN105724054A - Liquid culture solution for dried cordyceps militaris and culture method for dried cordyceps militaris - Google Patents
Liquid culture solution for dried cordyceps militaris and culture method for dried cordyceps militaris Download PDFInfo
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- CN105724054A CN105724054A CN201610097333.XA CN201610097333A CN105724054A CN 105724054 A CN105724054 A CN 105724054A CN 201610097333 A CN201610097333 A CN 201610097333A CN 105724054 A CN105724054 A CN 105724054A
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- China
- Prior art keywords
- silkworm
- cordyceps sinensis
- cordyceps militaris
- useless
- vitamin
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
Abstract
The invention discloses a liquid culture solution for dried cordyceps militaris and a culture method for dried cordyceps militaris. The culture solution is prepared from 9-10 g/L of a nitrogen source, 20-25 g/L of glucose, 0.9-1.2 g/L of citric acid triamine, 0.9-1.2 g/L of magnesium sulfate, 3-3.5 g/L of monopotassium phosphate, 1.2-1.8 g/L of salt and 60-80 mg/L of vitamins. The culture method comprises the following steps that waste silkworm bodies generated after a silk wadding quilt is processed are collected; the culture solution is dropwise added into the waste silkworm bodies, a bottle is sealed, and the waste silkworm bodies are boiled, disinfected and sterilized; inoculation is performed; cordyceps militaris grow. Due to the fact that the salt is added into the adopted culture solution, the C-N ratio in the culture solution is more balanced, and growth of cordyceps militaris is promoted. Meanwhile, by the adoption of the culture method, compared with the prior art, the production cost of cordyceps militaris is greatly lowered, in addition, the cultured cordyceps militaris contains no infectious microbes, and the nutritive value of infectious microbe is high.
Description
Technical field
The present invention relates to a kind of Cordyceps Cultivating techniques field, particularly to the cultural method of the liquid medium of a kind of dry silkworm chrysalis Cordyceps sinensis and dry silkworm chrysalis Cordyceps sinensis.
Background technology
In prior art, in order to solve the problems such as natural resources parasitic rate rare, expensive, natural existing for wild cordyceps is low, at present, country vigorously supports artificial culture's silkworm chrysalis Cordyceps sinensis.And in the process of artificial culture's silkworm chrysalis Cordyceps sinensis, it is necessary to adopt living silkworm chrysalises, and the price of living silkworm chrysalises is approximately in 200 yuan/kilogram, and technical matters is complicated, technology is difficult to popularize., inoculating on living silkworm chrysalises meanwhile, require higher to inoculation maneuver, the rigid rate of significant living silkworm chrysalises, to go out carelessness all relatively low, thus result in adopt the method cultivate needed for silkworm chrysalis Cordyceps sinensis relatively costly, be not easy to popularize.And when Cordyceps militaris (L.) Link. is cultivated in inoculation on dry Pupa bombycis, the dry Pupa bombycis because collecting contains certain alkali, causing that a bacterium rate of Cordyceps is low, in the culture fluid simultaneously adopted, C, N ratio is difficult to equilibrium, is also unfavorable for the growth of Cordyceps.
Summary of the invention
It is an object of the invention to overcome the deficiencies in the prior art, the liquid medium of a kind of dry silkworm chrysalis Cordyceps sinensis and the cultural method of dry silkworm chrysalis Cordyceps sinensis are provided, in this liquid medium, C, N are than equilibrium, be conducive to the growth of Cordyceps, adopt the silkworm chrysalis Cordyceps sinensis that this breeding method prepares simultaneously, its nutritional labeling is high, input cost is low, has a extensive future, has simultaneously effective carried out waste material utilization.
For reaching above-mentioned purpose, the technical solution used in the present invention is: the liquid medium of a kind of dry silkworm chrysalis Cordyceps sinensis, and described culture fluid is made up of each component of following content: nitrogenous source 9 ~ 10g/L, glucose 20 ~ 25g/L, triammonium citrate 0.9 ~ 1.2g/L, magnesium sulfate 0.9 ~ 1.2g/L, potassium dihydrogen phosphate 3 ~ 3.5g/L, Sal 1.2 ~ 1.8g/L, vitamin 60 ~ 80mg/L.
Preferably, the concentration of described Sal is 1.2 ~ 1.5g/L.
Preferably, one or more the combination in peptone, Carnis Bovis seu Bubali cream, yeast extract of the described nitrogenous source.
Preferably, described vitamin includes vitamin B
1
With vitamin B
12
, described vitamin B
1
With vitamin B
12
The mass ratio that feeds intake be 0.75 ~ 1.3:1.
It is a further object to provide the cultural method of a kind of dry silkworm chrysalis Cordyceps sinensis, specifically comprise the following steps that
1) collect the useless silkworm body after processed filament cotton quilt, be placed in clear water to soak, alkali and impurity are removed in rinsing, choose except the useless silkworm body gone rotten；
2) the useless silkworm body after step 1) being processed is put in the inoculation bottle after sterilization, and drips culture fluid, makes useless silkworm body absorb described culture fluid, then seals bottle；
3) will be equipped with the described inoculation bottle heated and boiled of useless silkworm body, and be incubated 10 ~ 15h, be then cooled to 20 ~ 15 DEG C, and sterilization treatment；
4) strain is seeded on the useless silkworm body in the described inoculation bottle after step 3) processes, temperature be 22 ~ 25 DEG C, humidity be 50 ~ 70% aseptic darkroom in a bacterium, cover with mycelia to silkworm surface；
5) the useless silkworm body covering with mycelia is delivered in cultivation workshop and carry out cultivation and grow grass.
Preferably, in step 5), in period of growing grass, control intensity of illumination is 2000 ~ 2500lx, and light application time is every 2 ~ 3 days, irradiates 48 ~ 72 hours.
Utilization due to technique scheme, the present invention compared with prior art has the advantage that the liquid medium of the dry silkworm chrysalis Cordyceps sinensis of the present invention, by adding Sal in its formula, effectively have adjusted C, N ratio in culture fluid so that Cordyceps more easily absorbs nutrient in growth course, simultaneously the breeding method of the dry silkworm chrysalis Cordyceps sinensis of the present invention, have employed in processed filament cotton quilt process, the useless silkworm body discarded, washes away its contained alkali and impurity by clear water, and what be conducive to the raising later stage goes out carelessness；Meanwhile, before inoculation, useless silkworm body is immersed in culture fluid, and heating disinfection, sterilizing, not only increase the content of the nutritional labeling of later stage silkworm chrysalis Cordyceps sinensis, be also effectively prevented from the generation of miscellaneous bacteria simultaneously.
Detailed description of the invention
Below in conjunction with specific embodiment, technical scheme is further elaborated.
Embodiment 1
The liquid medium of a kind of dry silkworm chrysalis Cordyceps sinensis, this culture fluid is prepared as follows: add peptone 9g, glucose 22g, triammonium citrate 1g, magnesium sulfate 0.9g, potassium dihydrogen phosphate 3.5g, Sal 1.5g, vitamin B in 1L water
1
30mg, vitamin B
12
40mg。
The incubation that above-mentioned culture fluid carries out dry silkworm chrysalis Cordyceps sinensis is adopted to be:
1) collect the useless silkworm body after processed filament cotton quilt, be placed in clear water to soak, alkali and impurity are removed in rinsing, choose except the useless silkworm body gone rotten；
2) the useless silkworm body after step 1) being processed is put in the inoculation bottle after sterilization, and drips culture fluid, makes useless silkworm body absorb culture fluid, then seals bottle；
3) will be equipped with the inoculation bottle heated and boiled of useless silkworm body, and be incubated 10 ~ 15h, be then cooled to 20 DEG C, and sterilization treatment；
4) strain is seeded on the useless silkworm body in the inoculation bottle after step 3) processes, temperature be 22 ~ 25 DEG C, humidity be 60 ~ 70% aseptic darkroom in a bacterium, cover with mycelia to silkworm surface；
5) being delivered to by the useless silkworm body covering with mycelia in cultivation workshop and carry out cultivation and grow grass, grow grass period, controls intensity of illumination is 2000 ~ 2200lx, and light application time is every irradiation in 3 days 72 hours.
After tested, without miscellaneous bacteria in silkworm chrysalis Cordyceps sinensis obtained in embodiment 1, containing cordycepin 9.35mg/g, adenosine 1.93mg/g, polysaccharide 3.89mg/g.
Embodiment 2
The liquid medium of a kind of dry silkworm chrysalis Cordyceps sinensis, this culture fluid is prepared as follows: add Carnis Bovis seu Bubali cream 10g, glucose 20g, triammonium citrate 1.2g, magnesium sulfate 1g, potassium dihydrogen phosphate 3.2g, Sal 1.8g, vitamin B in 1L water
1
40mg, vitamin B
12
40mg。
The incubation that above-mentioned culture fluid carries out dry silkworm chrysalis Cordyceps sinensis is adopted to be:
1) collect the useless silkworm body after processed filament cotton quilt, be placed in clear water to soak, alkali and impurity are removed in rinsing, choose except the useless silkworm body gone rotten；
2) the useless silkworm body after step 1) being processed is put in the inoculation bottle after sterilization, and drips culture fluid, makes useless silkworm body absorb culture fluid, then seals bottle；
3) will be equipped with the inoculation bottle heated and boiled of useless silkworm body, and be incubated 10 ~ 15h, be then cooled to 15 DEG C, and sterilization treatment；
4) strain is seeded on the useless silkworm body in the inoculation bottle after step 3) processes, temperature be 23 ~ 25 DEG C, humidity be 50 ~ 60% aseptic darkroom in a bacterium, cover with mycelia to silkworm surface；
5) being delivered to by the useless silkworm body covering with mycelia in cultivation workshop and carry out cultivation and grow grass, grow grass period, controls intensity of illumination is 2200 ~ 2500lx, and light application time is every irradiation in 2 days 72 hours.
After tested, without miscellaneous bacteria in silkworm chrysalis Cordyceps sinensis obtained in embodiment 2, containing cordycepin 9.41mg/g, adenosine 1.88mg/g, polysaccharide 3.92mg/g.
Embodiment 3
The liquid medium of a kind of dry silkworm chrysalis Cordyceps sinensis, this culture fluid is prepared as follows: add yeast extract 10g, glucose 25g, triammonium citrate 1g, magnesium sulfate 0.9g, potassium dihydrogen phosphate 3g, Sal 1.2g, vitamin B in 1L water
1
40mg, vitamin B
12
30mg。
The incubation that above-mentioned culture fluid carries out dry silkworm chrysalis Cordyceps sinensis is adopted to be:
1) collect the useless silkworm body after processed filament cotton quilt, be placed in clear water to soak, alkali and impurity are removed in rinsing, choose except the useless silkworm body gone rotten；
2) the useless silkworm body after step 1) being processed is put in the inoculation bottle after sterilization, and drips culture fluid, makes useless silkworm body absorb culture fluid, then seals bottle；
3) will be equipped with the inoculation bottle heated and boiled of useless silkworm body, and be incubated 10 ~ 15h, be then cooled to 18 DEG C, and sterilization treatment；
4) strain is seeded on the useless silkworm body in the inoculation bottle after step 3) processes, temperature be 22 ~ 25 DEG C, humidity be 60 ~ 65% aseptic darkroom in a bacterium, cover with mycelia to silkworm surface；
5) being delivered to by the useless silkworm body covering with mycelia in cultivation workshop and carry out cultivation and grow grass, grow grass period, controls intensity of illumination is 2300 ~ 2500lx, and light application time is every irradiation in 2 days 48 hours.
After tested, without miscellaneous bacteria in silkworm chrysalis Cordyceps sinensis obtained in embodiment 3, containing cordycepin 9.22mg/g, adenosine 2.01mg/g, polysaccharide 3.89mg/g.
Above-described embodiment is only for illustrating technology design and the feature of the present invention; its object is to allow person skilled in the art will appreciate that present disclosure and to be carried out; can not limit the scope of the invention with this; all equivalences made according to spirit of the invention change or modify, and all should be encompassed in protection scope of the present invention.
Claims (6)
1. the liquid medium of a dry silkworm chrysalis Cordyceps sinensis, it is characterized in that, described culture fluid is made up of each component of following content: nitrogenous source 9 ~ 10g/L, glucose 20 ~ 25g/L, triammonium citrate 0.9 ~ 1.2g/L, magnesium sulfate 0.9 ~ 1.2g/L, potassium dihydrogen phosphate 3 ~ 3.5g/L, Sal 1.2 ~ 1.8g/L, vitamin 60 ~ 80mg/L.
2. the liquid medium of dry silkworm chrysalis Cordyceps sinensis according to claim 1, it is characterised in that the concentration of described Sal is 1.2 ~ 1.5g/L.
3. the liquid medium of dry silkworm chrysalis Cordyceps sinensis according to claim 1, it is characterised in that the described nitrogenous source one or more combination in peptone, Carnis Bovis seu Bubali cream, yeast extract.
4. the liquid medium of dry silkworm chrysalis Cordyceps sinensis according to claim 1, it is characterised in that described vitamin includes vitamin B1With vitamin B12, described vitamin B1With vitamin B12The mass ratio that feeds intake be 0.75 ~ 1.3:1.
5. adopt as arbitrary in claim 1 ~ 4 as described in the liquid medium of dry silkworm chrysalis Cordyceps sinensis carry out the cultural method of dry silkworm chrysalis Cordyceps sinensis, it is characterised in that specifically comprise the following steps that
1) collect the useless silkworm body after processed filament cotton quilt, be placed in clear water to soak, alkali and impurity are removed in rinsing, choose except the useless silkworm body gone rotten；
2) the useless silkworm body after step 1) being processed is put in the inoculation bottle after sterilization, and drips culture fluid, makes useless silkworm body absorb described culture fluid, then seals bottle；
3) will be equipped with the described inoculation bottle heated and boiled of useless silkworm body, and be incubated 10 ~ 15h, be then cooled to 20 ~ 15 DEG C, and sterilization treatment；
4) strain is seeded on the useless silkworm body in the described inoculation bottle after step 3) processes, temperature be 22 ~ 25 DEG C, humidity be 50 ~ 70% aseptic darkroom in a bacterium, cover with mycelia to silkworm surface；
5) the useless silkworm body covering with mycelia is delivered in cultivation workshop and carry out cultivation and grow grass.
6. the cultural method of dry silkworm chrysalis Cordyceps sinensis according to claim 5, it is characterised in that in step 5), in period of growing grass, control intensity of illumination is 2000 ~ 2500lx, and light application time is every 2 ~ 3 days, irradiates 48 ~ 72 hours.
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Citations (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1094091A (en) * | 1994-02-05 | 1994-10-26 | 李春燕 | The seed selection of cordyceps militaris excellent species and high yield cultivating method |
| CN1304995A (en) * | 2001-01-19 | 2001-07-25 | 浙江大学 | Process for culturing cordyceps with domestic silkworm |
| CN1772876A (en) * | 2005-09-30 | 2006-05-17 | 袁有宝 | Formulation of aweto culture medium and oxygenating culture method of aweto |
| CN1793317A (en) * | 2005-11-16 | 2006-06-28 | 广东省微生物研究所 | Pupa Cordyceps sinensis fruiting body using sulphur pupa as host and cultivating process thereof |
| CN101720627A (en) * | 2009-12-16 | 2010-06-09 | 江苏江南生物科技有限公司 | Method for culturing cordyceps militaris by living silkworm chrysalises |
| CN102301912A (en) * | 2011-07-01 | 2012-01-04 | 西藏月王生物技术有限公司 | Method for culturing Cordyceps militaris by using highland barley as main raw material |
| CN103270887A (en) * | 2013-05-13 | 2013-09-04 | 玄永男 | Cordyceps militaris factory-like cultivation technology |
| CN103430776A (en) * | 2013-09-04 | 2013-12-11 | 鲁东大学 | Silkworm pupa-worm grass culture method capable of improving yield and quality |
| CN104160873A (en) * | 2014-08-06 | 2014-11-26 | 赵胜华 | Cultivation and management method for cordyceps militaris |
| CN104164367A (en) * | 2014-08-01 | 2014-11-26 | 沁阳市西向食用菌研究所 | Dried silkworm cordyceps militaris and culture method thereof |
| CN104823714A (en) * | 2015-05-04 | 2015-08-12 | 聂晶石 | Large-scale cultivation method for cordyceps militaris |
| CN104982225A (en) * | 2015-07-02 | 2015-10-21 | 溧阳市天目湖农业发展有限公司 | Novel male silkworm cordyceps culturing process and method for culturing silkworm cordyceps parent species |
-
2016
- 2016-02-23 CN CN201610097333.XA patent/CN105724054A/en active Pending
Patent Citations (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1094091A (en) * | 1994-02-05 | 1994-10-26 | 李春燕 | The seed selection of cordyceps militaris excellent species and high yield cultivating method |
| CN1304995A (en) * | 2001-01-19 | 2001-07-25 | 浙江大学 | Process for culturing cordyceps with domestic silkworm |
| CN1772876A (en) * | 2005-09-30 | 2006-05-17 | 袁有宝 | Formulation of aweto culture medium and oxygenating culture method of aweto |
| CN1793317A (en) * | 2005-11-16 | 2006-06-28 | 广东省微生物研究所 | Pupa Cordyceps sinensis fruiting body using sulphur pupa as host and cultivating process thereof |
| CN101720627A (en) * | 2009-12-16 | 2010-06-09 | 江苏江南生物科技有限公司 | Method for culturing cordyceps militaris by living silkworm chrysalises |
| CN102301912A (en) * | 2011-07-01 | 2012-01-04 | 西藏月王生物技术有限公司 | Method for culturing Cordyceps militaris by using highland barley as main raw material |
| CN103270887A (en) * | 2013-05-13 | 2013-09-04 | 玄永男 | Cordyceps militaris factory-like cultivation technology |
| CN103430776A (en) * | 2013-09-04 | 2013-12-11 | 鲁东大学 | Silkworm pupa-worm grass culture method capable of improving yield and quality |
| CN104164367A (en) * | 2014-08-01 | 2014-11-26 | 沁阳市西向食用菌研究所 | Dried silkworm cordyceps militaris and culture method thereof |
| CN104160873A (en) * | 2014-08-06 | 2014-11-26 | 赵胜华 | Cultivation and management method for cordyceps militaris |
| CN104823714A (en) * | 2015-05-04 | 2015-08-12 | 聂晶石 | Large-scale cultivation method for cordyceps militaris |
| CN104982225A (en) * | 2015-07-02 | 2015-10-21 | 溧阳市天目湖农业发展有限公司 | Novel male silkworm cordyceps culturing process and method for culturing silkworm cordyceps parent species |
Non-Patent Citations (1)
| Title |
|---|
| 严泽湘等: "《虫草蛹虫草灰树花》", 31 July 2013 * |
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Application publication date: 20160706 |