CN105638244A - Silkworm cordyceps militaris culturing method - Google Patents
Silkworm cordyceps militaris culturing method Download PDFInfo
- Publication number
- CN105638244A CN105638244A CN201610037980.1A CN201610037980A CN105638244A CN 105638244 A CN105638244 A CN 105638244A CN 201610037980 A CN201610037980 A CN 201610037980A CN 105638244 A CN105638244 A CN 105638244A
- Authority
- CN
- China
- Prior art keywords
- silkworm
- cordyceps militaris
- grass
- breeding method
- embedding
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Mycology (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Botany (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Environmental Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Mushroom Cultivation (AREA)
Abstract
The invention provides a silkworm cordyceps militaris culturing method. The method comprises the steps that 1, cordyceps militaris mycelia are taken and inoculated into a liquid culture medium added with 4%-8% of silkworm powder, directed culturing is conducted, and cordyceps militaris bacterial liquid is obtained; 2, sterile embedding liquid is added to the cordyceps militaris bacterial liquid, the liquid is stirred to be uniform, thermostatic standing is conducted at 25 DEG C, and cordyceps militaris embedding bacterial liquid is prepared; 3, the embedding bacterial liquid is injected into the abdomens of domestic silkworms subjected to sterilization treatment under the sterile condition, the inoculation amount is 0.2 mL-0.25 mL, and the silkworms are put into a sterile environment of which the temperature is 8 DEG C, and the humidity is 50% after inoculation till the silkworms are naturally rigidified and hardened; 4, the rigidified and hardened silkworms are vertically buried into a glass bottle filled with fine sand in a head upward mode, the heads of the silkworms are exposed, culturing is conducted for about 15 days in a simulated environment with natural temperature changing, after primordium differentiation formation of sporophores at the heads of the silkworms is achieved, the sporophores are continuously cultured to grow for 4 cm-5 cm, silkworm cordyceps militaris is taken out and dried, and the finished product is obtained. By means of the silkworm cordyceps militaris culturing method, efficient culturing of the silkworm cordyceps militaris is achieved, the production process is simple, the silkworm survival rate is high, silkworm cordyceps militaris germination is rapid, the stability is high, and silkworm cordyceps militaris bodies are full.
Description
Technical field
The invention belongs to Cordyceps Cultivating techniques field, particularly relate to the breeding method of a kind of silkworm grass.
Background technology
Silkworm grass is a kind of by Cordyceps militaris (L.) Link. fungus infected silkworm larva and a kind of entomogenous fungi complex producing sporophore. Silkworm is to be easiest to raise, cultivate the Cordyceps carrier that the polypide of largest, form and Cordyceps is close; Silkworm grass is the succedaneum that Cordyceps is suitable, and silkworm is can the lepidopteran insects supported of the artificial word of high-volume, it is easy to large-scale planting, medical value are high.
After Cordyceps militaris (L.) Link. fungus is had certain anti-infection ability and silkworm to be vaccinated Cordyceps militaris (L.) Link. fungus by silkworm body, mortality rate is higher, it it is one of the biggest problem in silkworm grass production process, although existing a large amount of research about silkworm grass breeding method at present, it is substantially all based on common Cordyceps militaris (L.) Link. fungus infection 3 ~ 5 silkworm in age, exist inoculation the survival rate of silkworm, infected rate, ossify rate and go out grass success rate relatively low, the even not first-class weak point of silkworm grass outward appearance, make profile and natural cs gap excessive, the added value of production cost largely effecting on silkworm grass and products thereof.
Therefore, for difficult problems such as survival rate, infection rate after silkworm inoculation in reply silkworm grass production process are relatively low, it is necessary to silkworm grass breeding method is improved.
Summary of the invention
In order to after overcoming silkworm inoculation, survival rate is low, infection ossifys, and rate is low, go out the not first-class deficiency in grass position, the present invention provides the breeding method of a kind of silkworm grass.
The present invention realizes the purpose of the present invention by the following technical solutions: the breeding method of a kind of silkworm grass, comprises the following steps:
(1) post-directed training: take Cordyceps militaris (L.) Link. mycelium inoculation in the fluid medium adding 4% ~ 8% silkworm powder, when lucifuge, constant-temperature table is trained for a certain area, obtains filtering through sterile gauze after adaptable liquid spawn when silkworm body fluid similar environments, collects Cordyceps militaris (L.) Link. bacterium solution;
(2) bacterium solution embedding: add aseptic embedding liquid in Cordyceps militaris (L.) Link. bacterium solution, the rear 25 DEG C of constant temperature that stir stand, and prepare Cordyceps militaris (L.) Link. embedding bacterium solution;
(3) inoculation: embedding bacterium solution being injected on the right side of the silkworm abdominal part first segment of sterilized process under aseptic condition, inoculum concentration is 0.2mL��0.25mL, puts into 8 DEG C, cultivates until silkworm body is naturally rigid hardening under the gnotobasis of humidity 50% after inoculation;
(4) it is cultured to finished product: head-up vertically imbed rigid hardening silkworm body equipped with in the vial of fine sand, expose silkworm body head, cultivate about 15 days under the environment of the natural temperature differential change of simulation, after silkworm body head fruit body primordium is differentiated to form, continue under the environment of light intensity 180lx��250lx to cultivate sporophore length 4cm��5cm, taking-up silkworm grass, dries and obtains finished product.
In described step (1), silkworm powder be five age silkworm powder. Described five age silkworm powder refer to egg-incubation collect ants after according to silkworm age reasonable adjusting temperature range 23 DEG C��28 DEG C, humidity range is under the environment of 65% ~ 85%, feed common Folium Mori to the 3rd day five ages, and after through inactivation, micronizing obtains silkworm powder after lyophilization.
In described step (1), post-directed training condition is temperature 25 DEG C, shaking speed 150r/min. Described 1L fluid medium contains glucose 2%, Rhizoma Solani tuber osi leaches powder 0.6%, peptone 0.4%, yeast extract 0.1%, KH2PO40.1%��MgSO40.05%, vitamin B1 (VB1) 0.01% and chloromycetin 0.01%. In gained bacterium solution, Cordyceps militaris (L.) Link. fungus number reaches 3.49 �� 105cfu/mL��
In described step (2), aseptic embedding liquid is made up of chitosan and 0.5% acetum of 0.1% ~ 0.2%. The time that constant temperature stands is 2 hours��4 hours.
In described step (3), silkworm cleans ultra-vioket radiation sterilizing 1h behind silkworm surface with 0.2% ClO 2 solution, namely completes silkworm body sterilizing, not feeding during sterilizing.
In described step (4), fine sand sieves through 35 orders, and sterilized process, moisture 5%. The environment of the natural temperature differential change of described simulation, particularly as follows: control humidity between 60%��85%, regulates temperature 22 DEG C��25 DEG C daytime, regulates temperature 16 DEG C��20 DEG C night.
The invention has the beneficial effects as follows:
(1) fluid present invention culture medium adds five age silkworm powder, Cordyceps militaris (L.) Link. fungus is oriented cultivation by simulation silkworm endotrophic physiological environment alive, reduce the gap of inoculation bacterium solution physicochemical property and silkworm body fluid, promote the multiplication capacity of Cordyceps militaris (L.) Link. fungus, it is thus achieved that the liquid spawn that activity is relatively big, silkworm appeal is strong.
(2) present invention by adding embedding liquid in liquid spawn, effectively reduce alkaline digest liquid in silkworm body strain is affected, extend strain time to live in silkworm body, improve the survival rate of silkworm after inoculating, rigid infection rate, by the method, silkworm survival rate after inoculation is increased to more than 98%.
(3) present invention is cultivated by simulating natural environment, goes out carelessness and reaches more than 95%, it is thus achieved that polypide is full, be directed out grass and high-quality silkworm that sporophore is longer grass.
Detailed description of the invention
Following example are used merely to explain the present invention, and protection scope of the present invention is not intended to be limited to following example. The those of ordinary skill of described technical field, according to above present disclosure, all can realize the purpose of the present invention. Following example are mass percent.
1L fluid medium contains glucose 2%, Rhizoma Solani tuber osi leaches powder 0.6%, peptone 0.4%, yeast extract 0.1%, KH2PO40.1%��MgSO40.05%, vitamin B1 (VB1) 0.01% and chloromycetin 0.01%.
Embedding liquid: chitosan and 0.5% acetum by 0.1% ~ 0.2% form.
Embodiment 1
(1) breed silkworms: egg-incubation collect ants after according to silkworm age reasonable adjusting temperature range 23 DEG C��28 DEG C, humidity range under the environment of 65%��85%, feed common Folium Mori to the 3rd day five ages.
(2) silkworm powder is prepared: gained fifth instar larva micronizing after inactivation, lyophilization obtains silkworm powder.
(3) post-directed training: by the Cordyceps militaris (L.) Link. mycelium inoculation after screening rejuvenation in the fluid medium adding 4% silkworm powder, when lucifuge, 25 DEG C, 150r/min constant-temperature table train for a certain area 6 days, obtain filtering through sterile gauze after adaptable liquid spawn when silkworm body fluid similar environments, collect Cordyceps militaris (L.) Link. bacterium solution.
(4) bacterium solution embedding: add aseptic embedding liquid in Cordyceps militaris (L.) Link. bacterium solution by 11 (W/W) mass ratio, magnetic agitation uniformly rear 25 DEG C of constant temperature stand 3h, prepare silkworm straw bag and bury bacterium solution. Aseptic embedding liquid is made up of 0.1% chitosan and 0.5% acetum.
(5) silkworm body sterilizing: with ultra-vioket radiation sterilizing 1h behind 0.2% ClO 2 solution cleaning silkworm surface, period is feeding not.
(6) inoculation: using the asepsis injector of syringe needle internal diameter 0.45mm embedding bacterium solution to be injected on the right side of silkworm abdominal part first segment, inoculum concentration is 0.25mL, and after inoculation, silkworm survival rate is 98%. Put into 8 DEG C after inoculation, cultivate until silkworm body is naturally rigid hardening under the gnotobasis of humidity 50%.
(7) it is cultured to finished product: head-up vertically imbedded by rigid hardening silkworm body and sieve equipped with 35 orders, moisture 5%, in the vial of the fine sand of 120 DEG C of sterilizing 30min process, expose silkworm body head, regulating temperature 22 DEG C��25 DEG C (daytime), change in 16 DEG C��20 DEG C (night) scopes, control humidity to cultivate about 15 days under the environment of simulating nature difference variation between 60%��85%, silkworm body head fruit body primordium is differentiated to form, then continue under the environment of light intensity 180lx ~ 250lx to cultivate sporophore length 4cm ~ 5cm, taking-up silkworm grass, dry and obtain finished product.
Embodiment 2
(1) breed silkworms: egg-incubation collect ants after according to silkworm age reasonable adjusting temperature range 23 DEG C��28 DEG C, humidity range under the environment of 65%��85%, feed common Folium Mori to the 3rd day five ages.
(2) silkworm powder is prepared: gained fifth instar larva micronizing after inactivation, lyophilization obtains silkworm powder.
(3) post-directed training: by the Cordyceps militaris (L.) Link. mycelium inoculation after screening rejuvenation in the fluid medium adding 8% silkworm powder, when lucifuge, 25 DEG C, 150r/min constant-temperature table train for a certain area 6 days, obtain filtering through sterile gauze after adaptable liquid spawn when silkworm body fluid similar environments, collect Cordyceps militaris (L.) Link. bacterium solution.
(4) bacterium solution embedding: add aseptic embedding liquid in Cordyceps militaris (L.) Link. bacterium solution by 11 (W/W) mass ratio, magnetic agitation uniformly rear 25 DEG C of constant temperature stand 3h, prepare silkworm straw bag and bury bacterium solution. Aseptic embedding liquid is made up of 0.2% chitosan and 0.5% acetum.
(5) silkworm body sterilizing: with ultra-vioket radiation sterilizing 1h behind 0.2% ClO 2 solution cleaning silkworm surface, period is feeding not.
(6) inoculation: using the asepsis injector of syringe needle internal diameter 0.45mm embedding bacterium solution to be injected on the right side of silkworm abdominal part first segment, inoculum concentration is 0.25mL, and after inoculation, silkworm survival rate is 99%. Put into 8 DEG C after inoculation, cultivate until silkworm body is naturally rigid hardening under the gnotobasis of humidity 50%.
(7) it is cultured to finished product: head-up vertically imbedded by rigid hardening silkworm body and sieve equipped with 35 orders, moisture 5%, in the vial of the fine sand of 120 DEG C of sterilizing 30min process, expose silkworm body head, regulating temperature 22 DEG C��25 DEG C (daytime), change in 16 DEG C��20 DEG C (night) scopes, control humidity to cultivate about 15 days under the environment of simulating nature difference variation between 60%��85%, silkworm body head fruit body primordium is differentiated to form, then continue under the environment of light intensity 180lx��250lx to cultivate sporophore length 4cm��5cm, taking-up silkworm grass, dry and obtain finished product.
Embodiment 3
(1) breed silkworms: egg-incubation collect ants after according to silkworm age reasonable adjusting temperature range 23 DEG C��28 DEG C, humidity range under the environment of 65%��85%, feed common Folium Mori to the 3rd day five ages.
(2) silkworm powder is prepared: gained fifth instar larva micronizing after inactivation, lyophilization obtains silkworm powder.
(3) post-directed training: by the Cordyceps militaris (L.) Link. mycelium inoculation after screening rejuvenation in the fluid medium adding 7% silkworm powder, when lucifuge, 25 DEG C, 150r/min constant-temperature table train for a certain area 6 days, obtain filtering through sterile gauze after adaptable liquid spawn when silkworm body fluid similar environments, collect Cordyceps militaris (L.) Link. bacterium solution.
(4) bacterium solution embedding: add aseptic embedding liquid in Cordyceps militaris (L.) Link. bacterium solution by 11 (W/W) mass ratio, magnetic agitation uniformly rear 25 DEG C of constant temperature stand 3h, prepare silkworm straw bag and bury bacterium solution. Aseptic embedding liquid is made up of 0.15% chitosan and 0.5% acetum.
(5) silkworm body sterilizing: with ultra-vioket radiation sterilizing 1h behind 0.2% ClO 2 solution cleaning silkworm surface, period is feeding not.
(6) inoculation: using the asepsis injector of syringe needle internal diameter 0.45mm embedding bacterium solution to be injected on the right side of silkworm abdominal part first segment, inoculum concentration is 0.35mL, and after inoculation, silkworm survival rate is 98%. Put into 8 DEG C after inoculation, cultivate until silkworm body is naturally rigid hardening under the gnotobasis of humidity 50%.
(7) cultivate, finished product: rigid hardening silkworm body is head-up vertically imbedded and sieves equipped with 35 orders, moisture 5%, in the vial of the fine sand of 120 DEG C of sterilizing 30min process, expose silkworm body head, regulating temperature 22 DEG C��25 DEG C (daytime), change in 16 DEG C��20 DEG C (night) scopes, control humidity to cultivate about 15 days under the environment of simulating nature difference variation between 60%��85%, silkworm body head fruit body primordium is differentiated to form, then continue under the environment of light intensity 180lx��250lx to cultivate sporophore length 4cm��5cm, taking-up silkworm grass, dry and obtain finished product.
Embodiment 4
(1) breed silkworms: egg-incubation collect ants after according to silkworm age reasonable adjusting temperature range 23 DEG C��28 DEG C, humidity range under the environment of 65%��85%, feed common Folium Mori to the 3rd day five ages.
(2) silkworm powder is prepared: gained fifth instar larva micronizing after inactivation, lyophilization obtains silkworm powder.
(3) post-directed training: by the Cordyceps militaris (L.) Link. mycelium inoculation after screening rejuvenation in the fluid medium adding 6% silkworm powder, when lucifuge, 25 DEG C, 150r/min constant-temperature table train for a certain area 6 days, obtain filtering through sterile gauze after adaptable liquid spawn when silkworm body fluid similar environments, collect Cordyceps militaris (L.) Link. bacterium solution.
(4) bacterium solution embedding: add aseptic embedding liquid in Cordyceps militaris (L.) Link. bacterium solution by 11 (W/W) mass ratio, magnetic agitation uniformly rear 25 DEG C of constant temperature stand 3h, prepare silkworm straw bag and bury bacterium solution. Aseptic embedding liquid is made up of 0.2% chitosan and 0.5% acetum.
(5) silkworm body sterilizing: with ultra-vioket radiation sterilizing 1h behind 0.2% ClO 2 solution cleaning silkworm surface, period is feeding not.
(6) inoculation: using the asepsis injector of syringe needle internal diameter 0.45mm embedding bacterium solution to be injected on the right side of silkworm abdominal part first segment, inoculum concentration is 0.25mL, and after inoculation, silkworm survival rate is 99%. Put into 8 DEG C after inoculation, cultivate until silkworm body is naturally rigid hardening under the gnotobasis of humidity 50%.
(7) it is cultured to finished product: head-up vertically imbedded by rigid hardening silkworm body and sieve equipped with 35 orders, moisture 5%, in the vial of the fine sand of 120 DEG C of sterilizing 30min process, expose silkworm body head, regulating temperature 22 DEG C��25 DEG C (daytime), change in 16 DEG C��20 DEG C (night) scopes, control humidity to cultivate about 15 days under the environment of simulating nature difference variation between 60%��85%, silkworm body head fruit body primordium is differentiated to form, then continue under the environment of light intensity 180lx��250lx to cultivate sporophore length 4cm��5cm, taking-up silkworm grass, dry and obtain finished product.
Claims (10)
1. the breeding method of a silkworm grass, it is characterised in that comprise the following steps:
(1) post-directed training: take Cordyceps militaris (L.) Link. mycelium inoculation in the fluid medium adding 4% ~ 8% silkworm powder, when lucifuge, constant-temperature table is trained for a certain area, obtains filtering through sterile gauze after adaptable liquid spawn when silkworm body fluid similar environments, collects Cordyceps militaris (L.) Link. bacterium solution;
(2) bacterium solution embedding: add aseptic embedding liquid in Cordyceps militaris (L.) Link. bacterium solution, the rear 25 DEG C of constant temperature that stir stand, and prepare silkworm straw bag and bury bacterium solution;
(3) inoculation: embedding bacterium solution being injected on the right side of the silkworm abdominal part first segment of sterilized process under aseptic condition, inoculum concentration is 0.2mL��0.25mL, puts into 8 DEG C, cultivates until silkworm body is naturally rigid hardening under the gnotobasis of humidity 50% after inoculation;
(4) it is cultured to finished product: head-up vertically imbed rigid hardening silkworm body equipped with in the vial of fine sand, expose silkworm body head, cultivate about 15 days under the environment of the natural temperature differential change of simulation, after silkworm body head fruit body primordium is differentiated to form, continue under the environment of light intensity 180lx��250lx to cultivate sporophore length 4cm��5cm, taking-up silkworm grass, dries and obtains finished product.
2. silkworm according to claim 1 grass breeding method, it is characterised in that in described step (1), silkworm powder be five age silkworm powder.
3. the breeding method of silkworm according to claim 2 grass, it is characterized in that, described five age silkworm powder refer to egg-incubation collect ants after according to silkworm age regulate temperature range 23 DEG C��28 DEG C, humidity range is under the environment of 65% ~ 85%, feed common Folium Mori to the 3rd day five ages, and after through inactivation, micronizing obtains silkworm powder after lyophilization.
4. the breeding method of the silkworm grass according to claim 1 or 2 or 3, it is characterised in that in described step (1), post-directed training condition is temperature 25 DEG C, shaking speed 150r/min.
5. the breeding method of silkworm according to claim 4 grass, it is characterised in that described 1L fluid medium contains glucose 2%, Rhizoma Solani tuber osi leaches powder 0.6%, peptone 0.4%, yeast extract 0.1%, KH2PO40.1%��MgSO40.05%, vitaminB10 .01% and chloromycetin 0.01%.
6. the breeding method of silkworm according to claim 4 grass, it is characterised in that in described step (2), aseptic embedding liquid is made up of chitosan and 0.5% acetum of 0.1% ~ 0.2%.
7. the breeding method of silkworm according to claim 4 grass, it is characterised in that in described step (2), the time that constant temperature stands is 2 hours ~ 4 hours.
8. the breeding method of silkworm according to claim 4 grass, it is characterised in that in described step (3), silkworm ultra-vioket radiation sterilizing 1h behind 0.2% ClO 2 solution cleaning silkworm surface, namely complete silkworm body sterilizing, not feeding during sterilizing.
9. the breeding method of silkworm according to claim 4 grass, it is characterised in that in described step (4), fine sand sieves through 35 orders, and sterilized process, moisture 5%.
10. the breeding method of silkworm according to claim 9 grass, it is characterized in that, in described step (4), the environment of the natural temperature differential change of described simulation is particularly as follows: control humidity between 60% ~ 85%, regulate temperature 22 DEG C ~ 25 DEG C daytime, regulate temperature 16 DEG C ~ 20 DEG C night.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610037980.1A CN105638244B (en) | 2016-01-21 | 2016-01-21 | A kind of breeding method of silkworm grass |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610037980.1A CN105638244B (en) | 2016-01-21 | 2016-01-21 | A kind of breeding method of silkworm grass |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105638244A true CN105638244A (en) | 2016-06-08 |
| CN105638244B CN105638244B (en) | 2018-06-29 |
Family
ID=56486816
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610037980.1A Active CN105638244B (en) | 2016-01-21 | 2016-01-21 | A kind of breeding method of silkworm grass |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105638244B (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108094047A (en) * | 2017-12-29 | 2018-06-01 | 谢远泰 | A kind of suitable bat moth larvae infection parent species bacterial strain and northern Chinese caterpillar Fungus cultural method |
| CN108112423A (en) * | 2017-12-27 | 2018-06-05 | 湖南省蚕桑科学研究所 | A kind of method that emulated ecological environment earthing cultivates silkworm grass |
| CN108293606A (en) * | 2017-10-16 | 2018-07-20 | 常德炎帝生物科技有限公司 | A kind of soil cultivation method of Cordyceps militaris potted landscape |
| CN108496694A (en) * | 2018-03-28 | 2018-09-07 | 全家百（苏州）生物科技有限公司 | By generative propagation strain by silkworm cultivation at the method for silkworm grass |
| CN110604004A (en) * | 2019-09-20 | 2019-12-24 | 新疆农垦科学院 | Method for separating strains by dried morchella sporocarp |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1051388A (en) * | 1990-11-19 | 1991-05-15 | 广东省农业科学院蚕业研究所 | The method of artificial culture Brazil Chinese caterpillar fungus |
| KR100366033B1 (en) * | 1999-04-02 | 2003-01-15 | 이상몽 | A process for cultivation of Paecilomyces japonica in pupae of the Antheraea pernyi |
| CN101215527A (en) * | 2008-01-11 | 2008-07-09 | 丁啸南 | Method for cultivating silkworm chrysalis Cordyceps sinensis |
| CN101627700A (en) * | 2009-08-14 | 2010-01-20 | 郑杰辉 | Method for culturing solid cordyceps militars strain |
| CN103371056A (en) * | 2013-07-19 | 2013-10-30 | 鹤山市中春生物科技有限公司 | Cultivation method of Cordyceps militaris (pupa) |
-
2016
- 2016-01-21 CN CN201610037980.1A patent/CN105638244B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1051388A (en) * | 1990-11-19 | 1991-05-15 | 广东省农业科学院蚕业研究所 | The method of artificial culture Brazil Chinese caterpillar fungus |
| KR100366033B1 (en) * | 1999-04-02 | 2003-01-15 | 이상몽 | A process for cultivation of Paecilomyces japonica in pupae of the Antheraea pernyi |
| CN101215527A (en) * | 2008-01-11 | 2008-07-09 | 丁啸南 | Method for cultivating silkworm chrysalis Cordyceps sinensis |
| CN101627700A (en) * | 2009-08-14 | 2010-01-20 | 郑杰辉 | Method for culturing solid cordyceps militars strain |
| CN103371056A (en) * | 2013-07-19 | 2013-10-30 | 鹤山市中春生物科技有限公司 | Cultivation method of Cordyceps militaris (pupa) |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108293606A (en) * | 2017-10-16 | 2018-07-20 | 常德炎帝生物科技有限公司 | A kind of soil cultivation method of Cordyceps militaris potted landscape |
| CN108112423A (en) * | 2017-12-27 | 2018-06-05 | 湖南省蚕桑科学研究所 | A kind of method that emulated ecological environment earthing cultivates silkworm grass |
| CN108094047A (en) * | 2017-12-29 | 2018-06-01 | 谢远泰 | A kind of suitable bat moth larvae infection parent species bacterial strain and northern Chinese caterpillar Fungus cultural method |
| CN108094047B (en) * | 2017-12-29 | 2020-08-14 | 谢远泰 | Strain suitable for infecting parent strain with hepialus armoricanus larvae and cordyceps militaris cultivation method |
| CN108496694A (en) * | 2018-03-28 | 2018-09-07 | 全家百（苏州）生物科技有限公司 | By generative propagation strain by silkworm cultivation at the method for silkworm grass |
| CN108496694B (en) * | 2018-03-28 | 2021-06-11 | 苏州雷旦生物科技有限公司 | Method for culturing silkworm into silkworm cordyceps sinensis by sexual propagation strain |
| CN110604004A (en) * | 2019-09-20 | 2019-12-24 | 新疆农垦科学院 | Method for separating strains by dried morchella sporocarp |
| CN110604004B (en) * | 2019-09-20 | 2021-08-03 | 新疆农垦科学院 | Method for separating strains by dried morchella sporocarp |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105638244B (en) | 2018-06-29 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103430776B (en) | Silkworm pupa-worm grass culture method capable of improving yield and quality | |
| CN105638244A (en) | Silkworm cordyceps militaris culturing method | |
| CN102440149A (en) | Method for breeding cordyceps militaris by utilizing silkworm larvae | |
| CN103270887B (en) | Silkworm chrysalis northern Chinese caterpillar Fungus industrial cultivation technique | |
| CN103262751A (en) | Method for cultivating cordyceps militaris by using cordyceps militaris strains to infect silkworm larvae | |
| CN102907256B (en) | Box-type cultivation method of tussah pupa cordyceps militaris | |
| CN106613359A (en) | Cordyceps liquid cultivation medium bottle cultivation mushroom fruiting cultivation method | |
| CN108450231B (en) | A kind of bionical border expanding propagation method of wild Phellinus | |
| KR101160069B1 (en) | Production method of functional rice with medicinal materials in mushrooms cordyceps militaris | |
| CN102939855B (en) | The cultivating method of imitative wild cordyceps militaris | |
| CN101366345A (en) | Method for growing clear-white gold needle mushroom with feces of chicken as cultivation material | |
| CN107810781A (en) | A kind of method that silkworm living cultivates Cordyceps militaris | |
| CN105103948B (en) | A kind of breeding method of silkworm chrysalis Cordyceps sinensis | |
| KR102265286B1 (en) | Method for manufacturing cordyceps scorched rice using grain products | |
| CN101195805A (en) | Cordyceps sinensis epiphyte and artificial cultivation method thereof | |
| CN107667776A (en) | A kind of Household edible mushroom planting method | |
| CN107216178A (en) | A kind of true pleurotus cornucopiae cultural method | |
| CN101423801A (en) | Artificial culture method of Chinese caterpillar fungus | |
| CN103688760A (en) | Method for culturing artificial cordyceps sinensis by using yellow mealworms as carriers | |
| CN106034738A (en) | Method for manually cultivating silkworm chrysalis cordyceps cicadae by using silkworm chrysalis of domestic silkworms as hosts | |
| CN103865805B (en) | Fermented black aspergillus suppresses the purposes of tobacco ralstonia solanacearum | |
| KR100723068B1 (en) | Method for culturing flammulina velutipes including ginseng saponin | |
| CN103843580A (en) | Paecilomyces hepialid mycelium and preparation method thereof | |
| CN107637390A (en) | A kind of live body silkworm and the method for the wild cicada fungus of silkworm chrysalis culture | |
| CN108370816A (en) | A kind of production method of rejuvenation Cordyceps militaris spawn |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |