CN107667776A - A kind of Household edible mushroom planting method - Google Patents
A kind of Household edible mushroom planting method Download PDFInfo
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- CN107667776A CN107667776A CN201710906491.XA CN201710906491A CN107667776A CN 107667776 A CN107667776 A CN 107667776A CN 201710906491 A CN201710906491 A CN 201710906491A CN 107667776 A CN107667776 A CN 107667776A
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Abstract
The present invention relates to field of edible fungus culture, more specifically, is related to a kind of Household edible mushroom planting method, carries out edible mashroom cultivating first, obtain edible fungus species, and the edible fungus species then are carried out into growing and cultivation at planting tray both ends；Edible fungus species are produced by way of three-level progressively expands culture, and the edible mashroom cultivating includes one-level kind culture medium, second-generation culture medivm and three-level kind culture medium and prepared；Carried out in one-level kind medium slant docking kind, culture, screening purification obtain original species, determine one-level kind through overtesting；One-level kind is inoculated with second-generation culture medivm, cultivated, obtains second class inoculum；Second class inoculum is inoculated with three-level kind culture medium, cultivated, obtains edible fungus species.Present invention can assure that the quality of strain, improves edible fungus culturing success rate.
Description
Technical field
The present invention relates to field of edible fungus culture, more specifically, is related to a kind of Household edible mushroom planting method.
Background technology
The bacterial initial species making of edible mushroom is the premise of edible fungus culturing, and the original seed that purity is high, vitality is strong is edible mushroom
Cultivation obtains high-quality prerequisite of getting bumper crops, and the bacterium germination management in later stage, management of producing mushroom, harvesting and bacteria are also all built upon strain
On the basis of, thus strain manufacturing technology is highly important.Market purchasing strain or bacterium bag cost are too big, the cell age of strain
It can not also ensure with inoculum concentration, the edible mushroom quality cultivated also can not just ensure.If oneself can make qualified strain,
The success rate of cultivation can improve.
The content of the invention
In the presence of overcoming the shortcomings of prior art, the present invention provides a kind of Household edible mushroom planting method, eats
The quality of bacterium strain is related to the yield and quality of edible fungus culturing, and it is qualified that edible mushroom makes, and can improve the success rate of cultivation.
In order to solve the above-mentioned technical problem, the technical solution adopted in the present invention is：
A kind of Household edible mushroom planting method, carries out edible mashroom cultivating first, obtains edible fungus species, then by the edible fungi
Kind carries out growing and cultivation at planting tray both ends；
The edible mashroom cultivating includes one-level kind culture medium, second-generation culture medivm and three-level kind culture medium and prepared；Trained in one-level kind
Support base inclined-plane on carry out docking kind, culture, screening purification obtain original species, determine one-level kind through overtesting；By one-level kind two
It is inoculated with, cultivated on level kind culture medium, obtains second class inoculum；Second class inoculum is inoculated with three-level kind culture medium, trained
Support, obtain edible fungus species.
Prepared by the one-level kind culture medium refers to：First add boiling to boil potato, filtered through gauze is used after then picking up, is taken
Juice；Agar is added in juice, glucose is added after agar dissolve fully, filtered through gauze is used after boiling, takes its juice；By juice
Load in teat glass, and stoppered and be placed in pressure cooker with cotton, sterilized under 98~108 kPas of pressure；After sterilizing in time
Take out, while hot tiltedly arrange test tube in becoming one-level kind culture medium on desktop, after cooling.
The original species separation refers to：The kind mushroom that will be sterilized, half-and-half breaks into two with one's hands, or use blade in inoculating hood at mushroom handle
Cut, form mushroom body split；At cap and stem intersection or lamella, a fritter mushroom body is cut with vaccinating lancet, is then indulged
Flakelet is cut into, with the fritter of transfer needle picking one, the center of one-level kind culture medium is accessed, treats that it sprouts mycelia, you can obtain original
Begin kind.
Prepared by the second-generation culture medivm refers to：Weed tree sawdust, wheat bran, sucrose, land plaster and magnesium sulfate are mixed and mixed thoroughly, is filled
Enter in glass seed bottle, charging requires that lower pine tightens；Bottleneck stoppers cotton, then encases bottleneck and tampon with brown paper；Then put
In in high-pressure sterilizing pot, sterilized under 147 kpa pressures；Kept for 10~11 hours at 100 DEG C, take out, allow while hot after up to standard
It is cooled down.
Compared with prior art, the advantageous effect of present invention is that：
The present invention replaces chemicals using potato, can gather materials on the spot, and economy is convenient, rich by the use of potato as culture medium nutrition
Richness, it is easy to be absorbed by mycelia, the metabolic demand of strain can be met；Agar is added in culture medium, is advantageous to make inclined-plane
Culture medium, facilitate the preservation in strain later stage；Cotton plug is screwed in test tube mouth, is ensureing the eubolism oxygen consumption premise of purpose strain
Under, prevent miscellaneous bacteria from infecting.Present invention can assure that the quality of strain, improves edible fungus culturing success rate.
Brief description of the drawings
Fig. 1 is the process chart of the present invention.
Embodiment
Below in conjunction with the accompanying drawing in the embodiment of the present invention, the technical scheme in the embodiment of the present invention is carried out clear, complete
Site preparation describes, it is clear that described embodiment is only part of the embodiment of the present invention, rather than whole embodiments.It is based on
Embodiment in the present invention, those of ordinary skill in the art are obtained every other under the premise of creative work is not made
Embodiment, belong to the scope of protection of the invention.
As shown in figure 1, a kind of Household edible mushroom planting method, carries out edible mashroom cultivating first, edible fungus species are obtained, so
The edible fungus species are subjected to growing and cultivation at planting tray both ends afterwards；
Edible fungus species are produced by way of three-level progressively expands culture, and strain production routine is divided into parent species, original seed
And cultigen, that is, usually said one-level, two level, three-class strain.The isolation and selection of wherein parent species is technical strong, it is desirable to essence
Change；And the making of original seed and cultigen is then fairly simple.The edible mashroom cultivating includes one-level kind culture medium, second-generation culture medivm
Prepared with three-level kind culture medium；Carried out in one-level kind medium slant docking kind, culture, screening purification obtain original species, pass through
Overtesting determines one-level kind；One-level kind is inoculated with second-generation culture medivm, cultivated, obtains second class inoculum；By two level bacterium
Kind is inoculated with three-level kind culture medium, cultivated, and obtains edible fungus species.
From wild or artificial cultivation colony, representational excellent mushroom body is selected as introduces a collection.Planting the standard of mushroom is：Eight
Maturation, piece shape circle just, fleshy hypertrophy, no disease and pests harm.The 1--2 kind mushroom for meeting above-mentioned standard of collection is numbered with number, as separation
The material of parent species.Opportunistic pathogen strain code name should be indicated from cultivating chamber collection.
In the present embodiment, prepared by one-level kind culture medium refers to：First boiling is added to boil potato, with gauze mistake after then picking up
Filter, takes juice；Agar is added in juice, glucose is added after agar dissolve fully, filtered through gauze is used after boiling, takes its juice；
Juice is loaded in teat glass, and is stoppered and is placed in pressure cooker with cotton, is sterilized under 98~108 kPas of pressure；Sterilizing
Take out, while hot tiltedly arrange test tube in becoming one-level kind culture medium on desktop, after cooling, this slant medium is convenient to be protected in time afterwards
Deposit.
In the present embodiment, original species separation refers to：The kind mushroom that will be sterilized, half-and-half breaks into two with one's hands in inoculating hood at mushroom handle,
Or with cut, form mushroom body split；At cap and stem intersection or lamella, a fritter mushroom is cut with vaccinating lancet
Body, then rip cutting, with the fritter of transfer needle picking one, access the center of one-level kind culture medium, treat that it sprouts mycelia, i.e., into flakelet
It can obtain original species.
The parent species of isolation and selection, it is necessary to carry out fruiting experiment.Method is：Parent species are inoculated in bottle or the training of packed wood chip
Support on base, according to requirement of the various mushroom ear kinds to temperature, carry out thermophilic culture, until fruiting just confirms to can be used for producing.
Access strain, when material temperature is down to less than 25 DEG C, aseptically, by test tube kind be divided into it is some determine, pass through
Alcolhol burner flame is accessed on pedigree seed culture medium rapidly, and every parent species can be by 4--6 bottle original seeds.
Glass seed bottle is moved into 25 DEG C of Spawn incubation rooms in time after inoculation and cultivated.Space relative humidity control exists
More than 70 DEG C, avoid strong illumination.The Primary spawn time, general mushroom class needed 40--50 days, and straw mushroom, white fungus only need 15--20 days
.
In the present embodiment, prepared by second-generation culture medivm refers to：Weed tree sawdust 77.5%, wheat bran 20%, sucrose 1%, land plaster
1.2%, magnesium sulfate 0.3%.PH value (before sterilizing) 6.5--7, water content are adjusted to 60%.Above-mentioned former, auxiliary material is mixed and mixed thoroughly, is filled
Enter in 750 milliliters of glass seed bottle, charging requires that lower pine tightens.Bottleneck stoppers cotton, then encases bottleneck and cotton with brown paper
Plug.It is subsequently placed in high-pressure sterilizing pot, is sterilized under 147 kpa pressures 2--2.5 hours.Also normal-pressure sterilization can be carried out, 100
10--11 hours are kept at DEG C, takes out, is allowed to cool while hot after up to standard.
Original seed is expanded again and is connected on same sawdust medium, it is cultigen that 30--35 days are cultivated under the conditions of thermophilic
(being called production kind), cultivates available for production.Cultigen can use 750 milliliters of strain special-purpose bottles, because usage amount is big, so generally
Use polypropylene strain bag more.The specification of bag has 12 centimetres x24 centimetres, 15 centimetres x28 centimetres, 17 centimetres x33 centimetres etc., can root
Used according to various mushroom ear characteristics and local custom selection.Medium sterilization is sterilized using atmospheric sterilizing stove, it is desirable to which sterilising temp reaches
100 DEG C, keep 8--10 hours, it is up to standard after while hot bleed cool down.When material temperature is down to below 25 DEG C, aseptically access
The original seed of selected kind.Every bottle of original seed, which can expand, is connected into 50--60 bottle cultigens.Breeding strain room requires clean, anti glare anti static coatingses, room temperature
23--25 DEG C, and running check are rested in, it is found that living contaminants should be eliminated in time, constantly selects excellent rogue.When the long purseful of mycelia, point
End mycelia reversion swash 1--3 centimetres when, be of the right age cultigen.
Only presently preferred embodiments of the present invention is explained in detail above, but the present invention is not limited to above-described embodiment,
In those of ordinary skill in the art's possessed knowledge, it can also be made on the premise of present inventive concept is not departed from each
Kind change, various change should be included in the scope of the protection.
Claims (4)
- A kind of 1. Household edible mushroom planting method, it is characterised in that carry out edible mashroom cultivating first, obtain edible fungus species, so The edible fungus species are subjected to growing and cultivation at planting tray both ends afterwards；The edible mashroom cultivating includes one-level kind culture medium, second-generation culture medivm and three-level kind culture medium and prepared；Trained in one-level kind Support base inclined-plane on carry out docking kind, culture, screening purification obtain original species, determine one-level kind through overtesting；By one-level kind two It is inoculated with, cultivated on level kind culture medium, obtains second class inoculum；Second class inoculum is inoculated with three-level kind culture medium, trained Support, obtain edible fungus species.
- A kind of 2. Household edible mushroom planting method according to claim 1, it is characterised in that：The one-level kind culture medium system It is standby to refer to：First add boiling to boil potato, filtered through gauze is used after then picking up, takes juice；Agar is added in juice, treats that agar is abundant Glucose is added after dissolving, filtered through gauze is used after boiling, takes its juice；Juice is loaded in teat glass, and stoppered with cotton It is placed in pressure cooker, is sterilized under 98~108 kPas of pressure；Take out, while hot tiltedly arrange test tube on desktop in time after sterilizing, One-level kind culture medium is become after cooling.
- A kind of 3. Household edible mushroom planting method according to claim 1, it is characterised in that also separated including original species, The original species separation refers to：The kind mushroom that will be sterilized, half-and-half breaks into two with one's hands in inoculating hood at mushroom handle, or with cut, makes Mushroom body forms split；At cap and stem intersection or lamella, a fritter mushroom body is cut with vaccinating lancet, then rip cutting is into little Bao Piece, with the fritter of transfer needle picking one, the center of one-level kind culture medium is accessed, treats that it sprouts mycelia, you can obtain original species.
- A kind of 4. Household edible mushroom planting method according to claim 1, it is characterised in that the second-generation culture medivm system It is standby to refer to：Weed tree sawdust, wheat bran, sucrose, land plaster and magnesium sulfate are mixed and mixed thoroughly, is loaded in glass seed bottle, under charging requires Pine tightens；Bottleneck stoppers cotton, then encases bottleneck and tampon with brown paper；It is subsequently placed in high-pressure sterilizing pot, in 147 kPas of pressures Sterilized under power；Kept for 10~11 hours at 100 DEG C, take out, be allowed to cool while hot after up to standard.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108633627A (en) * | 2018-08-13 | 2018-10-12 | 梅州市佳诚食用菌有限公司 | The method of cultured mycelia |
| CN109042075A (en) * | 2018-09-14 | 2018-12-21 | 段家忠 | Fang County's local ecosystem edible fungus species raising technology |
| CN112931043A (en) * | 2019-11-26 | 2021-06-11 | 江苏红叶福茸农业科技有限公司 | A method for preparing strain of Lentinus Edodes, Hericium Erinaceus, Ganoderma, etc |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102845225A (en) * | 2012-09-26 | 2013-01-02 | 济南蓬生农业科技有限公司 | Hypsizygus marmoreus liquid strain fermenting technique |
| CN102960184A (en) * | 2012-11-22 | 2013-03-13 | 兴隆县庆旺富硒食用菌农民专业合作社 | Edible fungus strain rich in organic selenium, preparation method therefore, edible fungus rich in organic selenium and culturing method thereof |
| KR20160088731A (en) * | 2015-01-16 | 2016-07-26 | (주)에코맘의 산골이유식 농업회사법인 | Method for preparing fermentative metabolites for enhancing immune activity |
| CN205755724U (en) * | 2016-05-25 | 2016-12-07 | 禄劝琼程美农业科技发展有限公司 | A kind of drawing and pulling type domestic planting edible mushroom case |
| CN106367355A (en) * | 2016-08-31 | 2017-02-01 | 河南省新乡市农业科学院 | Formula of secondary edible fungus strain and preparation process |
| CN106856992A (en) * | 2017-04-14 | 2017-06-20 | 西南大学 | A kind of method that utilization bamboo grove discarded object produces edible mushroom |
-
2017
- 2017-09-29 CN CN201710906491.XA patent/CN107667776A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102845225A (en) * | 2012-09-26 | 2013-01-02 | 济南蓬生农业科技有限公司 | Hypsizygus marmoreus liquid strain fermenting technique |
| CN102960184A (en) * | 2012-11-22 | 2013-03-13 | 兴隆县庆旺富硒食用菌农民专业合作社 | Edible fungus strain rich in organic selenium, preparation method therefore, edible fungus rich in organic selenium and culturing method thereof |
| KR20160088731A (en) * | 2015-01-16 | 2016-07-26 | (주)에코맘의 산골이유식 농업회사법인 | Method for preparing fermentative metabolites for enhancing immune activity |
| CN205755724U (en) * | 2016-05-25 | 2016-12-07 | 禄劝琼程美农业科技发展有限公司 | A kind of drawing and pulling type domestic planting edible mushroom case |
| CN106367355A (en) * | 2016-08-31 | 2017-02-01 | 河南省新乡市农业科学院 | Formula of secondary edible fungus strain and preparation process |
| CN106856992A (en) * | 2017-04-14 | 2017-06-20 | 西南大学 | A kind of method that utilization bamboo grove discarded object produces edible mushroom |
Non-Patent Citations (2)
| Title |
|---|
| 包水明: "《香菇地栽新技术》", 31 May 2014 * |
| 蔡津生: "《中国食药用菌工程学》", 31 January 2015 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108633627A (en) * | 2018-08-13 | 2018-10-12 | 梅州市佳诚食用菌有限公司 | The method of cultured mycelia |
| CN109042075A (en) * | 2018-09-14 | 2018-12-21 | 段家忠 | Fang County's local ecosystem edible fungus species raising technology |
| CN112931043A (en) * | 2019-11-26 | 2021-06-11 | 江苏红叶福茸农业科技有限公司 | A method for preparing strain of Lentinus Edodes, Hericium Erinaceus, Ganoderma, etc |
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Application publication date: 20180209 |