CN104521557A - Cultivating method for coralliform lucid ganoderma - Google Patents
Cultivating method for coralliform lucid ganoderma Download PDFInfo
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- CN104521557A CN104521557A CN201410774474.1A CN201410774474A CN104521557A CN 104521557 A CN104521557 A CN 104521557A CN 201410774474 A CN201410774474 A CN 201410774474A CN 104521557 A CN104521557 A CN 104521557A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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Abstract
The invention discloses a cultivating method for a coralliform lucid ganoderma, and relates to the technical field of the cultivation. The cultivating method for the coralliform lucid ganoderma comprises the steps of, under the aseptic condition, placing the lucid ganoderma sporocarp in 0.1% of mercury solution and soaking for 2 minutes, peeling the external shell of the sporocarp, cutting the bacterial content to small chips, and inoculating in a slant culture medium; loading basswoods into a cylinder bag, then placing in a sterilization device, quickly increasing the temperature of the sterilization device to 100 DEG C and keeping for 12 hours, and reducing the temperature of the sterilization device below 80 DEG C; after reducing the temperature of the sterilized cylinder bag to 28-30 DEG C, inoculating the mother strain in the basswoods; keeping the room temperature during the cultivation between 25 DEG C and 28 DEG C; ripening the hypha for 60-70 days, and concentrating the basswood bacteria sticks with grown primordium in a small house with good leakproofness, wherein the carbon dioxide content in the small house is 0.1%-0.8%, and the relative air humidity is 85%-90%. The cultivating method for the coralliform lucid ganoderma is capable of solving the problem that the lucid ganoderma has not the ornamental value.
Description
Technical field
The present invention relates to Cultivating techniques field, especially a kind of coralliform glossy ganoderma breeding method.
Background technology
Glossy ganoderma, has another name called polyporus lucidus, and in normal growth situation, cap has semicircle, kidney shape, circle, the various shape such as fan-shaped, above has ring-type, cloud form rib and radiation wrinkle, and stem side is raw or wilfully.Kind is different, and color has reddish brown, purple, dark violet, white, orange, indigo naturalis, pitch-dark multiple, hard, the lignification of quality after ripe, has and paints sample natural gloss.Although glossy ganoderma has very high medical value, it does not have sight.
Summary of the invention
The object of this invention is to provide a kind of coralliform glossy ganoderma breeding method, it can solve the problem that glossy ganoderma does not have sight.
In order to solve the problem, the technical solution used in the present invention is: this coralliform glossy ganoderma breeding method, carries out according to the following steps:
A, bacterial classification are produced: aseptically, are placed on by ganoderma lucidum fruitbody in 0.1% mercury solution and soak 2 minutes, taking-up aseptic water washing, then dry moisture with sterile-cloth; The outside cot of described fruit body of pruning, is cut into small pieces bacterial context, is seeded in slant medium, under dark condition, namely obtains female kind after 10 days;
B, cultivation basswood: (1), the trees cut latter about 12 days are planted the linden for length 14 centimetres ~ 15 centimetres or 28 centimetres ~ 30 centimetres; (2), by described linden load in cylinder bag, slip-knot is pricked respectively in described cylinder bag two ends; (3), sterilizing: the described cylinder bag that described linden is housed is put into bactericidal unit, makes the temperature of described bactericidal unit be raised to 100 degrees Celsius fast and keep 12 hours, then make the temperature of described bactericidal unit drop to less than 80 degrees Celsius, described cylinder bag is taken out;
C, inoculation: in good seal, place drying, clean indoor, make the identical multiple inoculation accounts of area with plastic foil, with disinfectant, described inoculation account is carried out disinfection before inoculation; When the temperature of the described cylinder bag after sterilizing is down to 28 degrees Celsius ~ 30 degrees Celsius, described mother is planted and is seeded on described linden; With 75% alcohol or 0.05% new clean that, disinfection is planted to described mother during inoculation;
D, cultural hypha: move between cultivation by connecting the described linden of planting, select the cob wall room of good heat insulating between described cultivation, room temperature is at 25 degrees Celsius ~ 28 degrees Celsius; Send out bacterium and will carry out turning after 20 days, will illumination be increased when mycelia covers with described cylinder bag; 60 ~ 70 days mycelia maturations, now, bacterium outer surface of wood layer mycelia is pure white sturdy, is close to and not easily breaks into two with one's hands between bacterium wood, and minority bacterium wood section has the large former base of beans to occur;
E, indoor culture: the linden bacterium rod of long described former base is focused on the within doors little of a good leak tightness, and close the doors and windows, described little carbon dioxide content is within doors 0.1% ~ 0.8%, and relative air humidity is 85% ~ 90%.
In technique scheme, more specifically scheme can also be: described medium is made up of following material by its weight: weed tree sawdust 77%, wheat bran 18%, corn flour 3%, sucrose 1%, land plaster 1%.
Further: described medium is made up of following material by its weight: cotton seed hulls 44%, weed tree sawdust 44%, wheat bran 5%, corn flour 5%, sucrose 1%, land plaster 1%.
Further: described medium is made up of following material by its weight: cotton seed hulls 44%, weed tree sawdust 44%, wheat bran 5%, corn flour 5%, sucrose 1%, land plaster 1%.
Further: described linden is the age of tree 7 ~ 10 years, the robur that tree-walk is 6 ~ 15 centimetres, elm, birch any one.
Further: described cylinder bag is 3.5 ~ 6 decimillimeters, inclined footpath width is that the polyethylene of 15 ~ 30 centimetres is made.
Owing to have employed technique scheme, the present invention compared with prior art, the beneficial effect had is: coralliform glossy ganoderma is by cultivating out to the control of glossy ganoderma growing environment condition, it not only has very high medical value, and can glossy ganoderma dish garden be made, be the wonderful work viewed and admired in fungi, market prospects are had an optimistic view of.
Embodiment
Below in conjunction with embodiment, the invention will be further described:
One, coralliform glossy ganoderma grow need condition
1, nutrition: coralliform glossy ganoderma is a kind of saprophytic bacteria is also facultative parasite.Coralliform glossy ganoderma has stronger decomposition and absorbing capacity to the material such as lignin, cellulose, adaptability is wider, most of broad-leaved tree and wood chip, leaf, agricultural crop straw, cotton seed hull, corncob etc., can as the raw material of medium with addition of appropriate wheat bran or bran.
2, temperature: the high temperatures mushroom of coralliform Ganoderma, all can grow between 15 ~ 35 DEG C, thermophilic is 30 ~ 35 DEG C.Fruit body all can grow in the scope of 10 ~ 32 DEG C.
3, humidity: the humidity that coralliform glossy ganoderma growth needs is higher.Growth period of hypha, require that moisture content in medium is 55% ~ 60%, relative air humidity is 70% ~ 80%.The fruit body development phase, relative air humidity requires 85% ~ 90%.
4, air: general glossy ganoderma is aerobic fungi, all needs ozone in its whole growth and development process.Coralliform glossy ganoderma is then very strict to api request, in its sporophore growth developmental stage, requires to carbon dioxide content 0.1% ~ 0.8%, and fruit body just not parachute-opening, grows up to coralliform.Utilize this principle that the bacterium bottle of long former base, bacterium bag and Duan Mu are focused on a seal within doors little preferably, close the doors and windows and allow it grow, through a large amount of coralliform glossy ganodermas just can be obtained after a while.
Two, cultivate: the cultivation method of coralliform glossy ganoderma mainly contains that bottle is planted, bag is planted, segment wood cultivated.Introduce segment wood cultivated method below:
1, bacterial classification is produced: the normal tissue isolation that adopts of coralliform lucid ganoderma stock culture preparation obtains pure mother's kind.Choose the tender tissue of not yet suberin.Aseptically, get glossy ganoderma tissue block and be placed in 0.1% mercuric chloride solution and carry out surface sterilizing, process 2 minutes, then use aseptic water washing, dry with sterile gauze.The outside cot of fruit body of pruning with scalpel, is cut into small pieces bacterial context, is seeded in slant medium, under being placed on dark condition, can cover with test tube through 10 days mycelia, be pure mother's kind.Switching original seed can be cultivated in order to expand.Spring plant mid or late May cultivation inoculation, mid or late April original seed; Autumn plant August cultivation inoculation, July original seed.The inoculation of section wood is generally carried out in April in spring.
2, medium: the kinds of culture medium being applicable to coralliform cultivation of glossy ganoderma is a lot, only introduces the most frequently used two kinds.
(1) weed tree sawdust 77%, wheat bran 18%, corn flour 3%, sucrose 1%, land plaster 1%.
(2) cotton seed hulls 44%, weed tree sawdust 44%, wheat bran 5%, corn flour 5%, sucrose 1%, land plaster 1%.
3. cultivation basswood method
(1), linden: select the deciduous species such as robur, elm, birch, the age of tree 7 ~ 10 years, tree-walk 6 ~ 15 centimetres.For preventing timber moisture from crossing wet or overdrying after weedtree felling, be generally advisable to cut latter about 12 days, by sawed-off for weedtree be length 14 ~ 15 centimetres or 28 ~ 30 centimetres of short lindens, no matter which kind of specification, must section smooth, length is consistent.
(2), pack: according to the size of linden diameter, select the polyethylene cylinder bag of high temperature resistant, the stretching resistance of inclined footpath width 15 ~ 30 centimetres of different sizes, thickness 3.5 ~ 6 decimillimeter respectively.Put linden by packed for suitable cylinder during pack, slip-knot is pricked respectively in two; Pack, remove bag and will handle with care, prevent breakage.
(3), sterilizing: sterilizing is the key that glossy ganoderma produces success, must steam foot well-done.12 hours to be maintained after bactericidal unit or kettle temperature reach 100 DEG C do not lower the temperature.Firepower is wanted " tap holder, keep in the middle of tail, control ", and bacterium cylinder is wanted after doing the cooking, rapidly with very hot oven onslaught, make kettle temperature reach rapidly 100 DEG C, middle stable fire, and after ceasing fire, temperature drops to less than 80 DEG C and goes out stove, cooling.
(4), inoculation: transfer room can select 10 ~ 15 square metres of rooms that doors and windows sealing is good, place is dry, clean, and equal inoculation account made by interior plastic foil, carried out disinfection by every cubic metre, inoculation account for 4 ~ 8 hours before inoculation with disinfectant.Temperature control inoculation when a bag temperature drop to 28 ~ 30 DEG C.During inoculation with 75% alcohol or 0.05% new clean you bacterial classification is conscientiously processed, carry out a people kind, many people Xie Kou inoculate prick bag, special messenger stack continuous productive process.Sowing will expire section, and mouth wants sturdy, and two is inoculated, and heap is kept steady.
(5), cultural hypha: the cob wall room selecting good heat insulating between cultivation, room temperature will control at about 25 DEG C.Fire pot as too low in temperature or coal, electricity heating; Temperature is too high will suitably ventilate, and prevents sultry burning bacterium.To try one's best between cultivation lucifuge, to send out a bacterium and turning as one sees fit after 20 days, will be carried out, suitably increase illumination when mycelia covers with full bag, promote mycelial growth.As found, bacterium internal water accumulation or mycelia atrophy must cut osculum venting at cylinder bag, with ventilate, and allow ponding run off.Cultivate 60 ~ 70 days mycelia maturations at 25 ~ 28 DEG C, bacterium outer surface of wood layer mycelia is pure white sturdy, is close to and not easily breaks into two with one's hands between bacterium wood, and minority bacterium wood section has the large former base of beans to occur.
(6) indoor culture: it is within doors little preferably that the section wood fungus rod of long former base focuses on a seal, close the doors and windows and allow it grow, require that carbon dioxide content is 0.1% ~ 0.8%, relative air humidity requires 85% ~ 90%, fruit body just not parachute-opening, grows up to coralliform.At the beginning of 7 months, fruit body occurs successively.Second Year abounds with for 5 ~ July, within the 3rd year, still has fruit body to produce.
Three, pick and process
When coralliform Ganoderma Lucidum periphery white growth circle disappears, thalline is bronzing, when there is coffee-like Powdered spore, can take fruit body.Generally about needing 50 ~ 60 days from being seeded to gather, adopting rear naturally drying or low temperature drying.
Claims (5)
1. a coralliform glossy ganoderma breeding method, is characterized in that: carry out according to the following steps:
A, bacterial classification are produced: aseptically, are placed on by ganoderma lucidum fruitbody in 0.1% mercury solution and soak 2 minutes, taking-up aseptic water washing, then dry moisture with sterile-cloth; The outside cot of described fruit body of pruning, is cut into small pieces bacterial context, is seeded in slant medium, under dark condition, namely obtains female kind after 10 days;
B, cultivation basswood: (1), the trees cut latter about 12 days are planted the linden for length 14 centimetres ~ 15 centimetres or 28 centimetres ~ 30 centimetres; (2), by described linden load in cylinder bag, slip-knot is pricked respectively in described cylinder bag two ends; (3), sterilizing: the described cylinder bag that described linden is housed is put into bactericidal unit, makes the temperature of described bactericidal unit be raised to 100 degrees Celsius fast and keep 12 hours, then make the temperature of described bactericidal unit drop to less than 80 degrees Celsius, described cylinder bag is taken out;
C, inoculation: in good seal, place drying, clean indoor, make the identical multiple inoculation accounts of area with plastic foil, with disinfectant, described inoculation account is carried out disinfection before inoculation; When the temperature of the described cylinder bag after sterilizing is down to 28 degrees Celsius ~ 30 degrees Celsius, described mother is planted and is seeded on described linden; With 75% alcohol or 0.05% new clean that, disinfection is planted to described mother during inoculation;
D, cultural hypha: move between cultivation by connecting the described linden of planting, select the cob wall room of good heat insulating between described cultivation, room temperature is at 25 degrees Celsius ~ 28 degrees Celsius; Send out bacterium and will carry out turning after 20 days, will illumination be increased when mycelia covers with described cylinder bag; 60 ~ 70 days mycelia maturations, now, bacterium outer surface of wood layer mycelia is pure white sturdy, is close to and not easily breaks into two with one's hands between bacterium wood, and minority bacterium wood section has the large former base of beans to occur;
E, indoor culture: the linden bacterium rod of long described former base is focused on the within doors little of a good leak tightness, and close the doors and windows, described little carbon dioxide content is within doors 0.1% ~ 0.8%, and relative air humidity is 85% ~ 90%.
2. coralliform glossy ganoderma breeding method according to claim 1, is characterized in that: described medium is made up of following material by its weight: weed tree sawdust 77%, wheat bran 18%, corn flour 3%, sucrose 1%, land plaster 1%.
3. coralliform glossy ganoderma breeding method according to claim 1, is characterized in that: described medium is made up of following material by its weight: cotton seed hulls 44%, weed tree sawdust 44%, wheat bran 5%, corn flour 5%, sucrose 1%, land plaster 1%.
4. the coralliform glossy ganoderma breeding method according to claim 1 or 2 or 3, is characterized in that: described linden is the age of tree 7 ~ 10 years, the robur that tree-walk is 6 ~ 15 centimetres, elm, birch any one.
5. the coralliform glossy ganoderma breeding method according to claim 1 or 2 or 3, is characterized in that: described cylinder bag is 3.5 ~ 6 decimillimeters, and inclined footpath width is that the polyethylene of 15 ~ 30 centimetres is made.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104871812A (en) * | 2015-04-29 | 2015-09-02 | 王海霞 | Cultivation method of high-yield and storage-resistant ganoderma lucidum |
| CN105210669A (en) * | 2015-08-28 | 2016-01-06 | 湖南省致远农业科技发展有限公司 | A kind of with the method for wild Chinese silvergrass for major ingredient factory culture glossy ganoderma |
| CN106550769A (en) * | 2016-11-25 | 2017-04-05 | 邓小坤 | Ganoderma Lucidum rod preparation method |
| CN106797799A (en) * | 2015-11-25 | 2017-06-06 | 浙江超奇农业开发有限公司 | A kind of cultural method of ganoderma lucidum |
| CN110089345A (en) * | 2019-06-10 | 2019-08-06 | 安徽金寨仙芝灵生物科技有限公司 | A kind of linden ganoderma lucidum soilless culture method |
| CN112493054A (en) * | 2020-11-23 | 2021-03-16 | 安徽农业大学 | Ganoderma lucidum culture material based on moso bamboo sawdust and preparation method and application thereof |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104871812A (en) * | 2015-04-29 | 2015-09-02 | 王海霞 | Cultivation method of high-yield and storage-resistant ganoderma lucidum |
| CN105210669A (en) * | 2015-08-28 | 2016-01-06 | 湖南省致远农业科技发展有限公司 | A kind of with the method for wild Chinese silvergrass for major ingredient factory culture glossy ganoderma |
| CN105210669B (en) * | 2015-08-28 | 2018-07-06 | 湖南省致远农业科技发展有限公司 | It is a kind of using wild Chinese silvergrass as the method for major ingredient factory culture ganoderma lucidum |
| CN106797799A (en) * | 2015-11-25 | 2017-06-06 | 浙江超奇农业开发有限公司 | A kind of cultural method of ganoderma lucidum |
| CN106550769A (en) * | 2016-11-25 | 2017-04-05 | 邓小坤 | Ganoderma Lucidum rod preparation method |
| CN110089345A (en) * | 2019-06-10 | 2019-08-06 | 安徽金寨仙芝灵生物科技有限公司 | A kind of linden ganoderma lucidum soilless culture method |
| CN112493054A (en) * | 2020-11-23 | 2021-03-16 | 安徽农业大学 | Ganoderma lucidum culture material based on moso bamboo sawdust and preparation method and application thereof |
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Application publication date: 20150422 |