CN105210669B - It is a kind of using wild Chinese silvergrass as the method for major ingredient factory culture ganoderma lucidum - Google Patents

It is a kind of using wild Chinese silvergrass as the method for major ingredient factory culture ganoderma lucidum Download PDF

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CN105210669B
CN105210669B CN201510534238.7A CN201510534238A CN105210669B CN 105210669 B CN105210669 B CN 105210669B CN 201510534238 A CN201510534238 A CN 201510534238A CN 105210669 B CN105210669 B CN 105210669B
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ganoderma lucidum
chinese silvergrass
days
bacterium bag
wild chinese
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CN105210669A (en
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彭程
李健
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HUNAN ZHIYUAN AGRICULTURE TECHNOLOGY Co Ltd
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HUNAN ZHIYUAN AGRICULTURE TECHNOLOGY Co Ltd
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Abstract

The present invention relates to a kind of method using wild Chinese silvergrass as major ingredient factory culture ganoderma lucidum.At present, for the formula that most domestic cultivation of glossy ganoderma uses using Duan Mu, sawdust, cotton seed hulls as primary carbon source, wheat bran, corn flour are main nitrogen.Wherein primary carbon source of the cotton seed hulls as Lucid ganoderma pocket planting, not only price is higher, and its middle peasant it is residual content it is also very high, it can not ensure the quality of ganoderma lucidum and the safety of food, containing abundant nutriment in Chinese silvergrass, wherein crude fiber content is up to 38.96%, and thick protein is up to 10.36%, Ca is up to 0.31%, P up to 0.57%;These nutriments are not utilized.The present invention is a kind of using wild Chinese silvergrass as the method for major ingredient factory culture ganoderma lucidum, includes the following steps:--- raw material weighs, and --- --- --- --- --- cultural hypha --- management of producing mushroom --- harvests fermenting raw materials for inoculation for cooling for sterilizing for spice pack.

Description

It is a kind of using wild Chinese silvergrass as the method for major ingredient factory culture ganoderma lucidum
Technical field:
The present invention relates to a kind of method of cultivating ganoderma, more particularly, to one kind using wild Chinese silvergrass as major ingredient factory culture The method of ganoderma lucidum.
Background technology:
1st, ganoderma lucidum has a long history culture, is the foremost edible and medicinal fungi in China.As legal Chinese medicine, ganoderma lucidum It is had been developed that in China and is developed into Chinese patent medicine preparation or health food, for preventing various diseases;It is external also ganoderma lucidum with going bail for Health food is eaten extensively.
2nd, in recent years, the cultivation of this rare ganoderma lucidum is at home and abroad concerned, and the cultural method of ganoderma lucidum is to its quality, production Amount and production cost etc. have direct influence.
3rd, at present, the method that cultivating ganoderma uses has with the segment wood cultivated method of Duan Muwei primary raw materials and with cotton seed hulls, wood Consider the bagging method for primary raw material to be worth doing, this two methods is greenhouse gardening.On the one hand, cotton seed hulls is as the main of Lucid ganoderma pocket planting Raw material, not only price is higher, and its middle peasant is residual and the content of heavy metal is all very high, can not ensure the quality and its food of ganoderma lucidum Safety.And as the wide segment wood cultivated method of production ganoderma lucidum, need to consume a large amount of timber, more than supply shortage, The forest reserves can be also greatly destroyed, the serious consequence that can not be reversed is caused to environment;On the other hand, traditional greenhouse cultivation spirit The method of sesame, pest and disease damage can not be effectively controlled, be can hardly be avoided using pesticide and chemical agent.And use wild Chinese silvergrass conduct Major ingredient factory culture ganoderma lucidum, can not only prevent the use of various pesticides and chemical agent, ensure that zero agriculture of product is residual, and It also is able to be significantly reduced the destruction to environment, wherein wild Chinese silvergrass, generally most of to be grown among wild deep woods, without any The pollution of pesticide, it may be said that belong to a kind of pure natural renewable organic plant, enormous amount, but at present due to not knowing its use On the way, most of cut by peasant household goes home to be used as burning of igniting.According to《Grass cultivation and herding》It reports, abundant nutrition is contained in Chinese silvergrass Substance, wherein crude fiber content are up to 38.96%, and thick protein is up to 10.36%, Ca up to 0.31%, P up to 0.57%;These nutrition Substance is not utilized, causes the waste of resource.Up to the present, there are no any one about wild Chinese silvergrass is used to make Report for major ingredient cultivating ganoderma method.
Invention content:
The technical problem to be solved by the present invention is to overcome the deficiencies of the prior art and provide a kind of novel material and realize factory Change the method for cultivating ganoderma.
The present invention solve its technical problem the technical solution adopted is that, it is a kind of clever by major ingredient factory culture of wild Chinese silvergrass The method of sesame, includes the following steps:--- raw material weighs, and --- --- sterilizing --- cooling --- connects fermenting raw materials for spice pack --- cultural hypha --- management of producing mushroom --- harvests kind;
Concrete operation step is as follows:
(1)Fermenting raw materials:Wild Chinese silvergrass and sawdust are crushed to average particle size as 10-20 mesh, after being irrigated with water, respectively into Row builds heap fermentation, wild Chinese silvergrass fermentation time 15-25 days, sawdust fermentation time 30-50 days, as ganoderma lucidum production medium master Material;
(2)Raw material weighs:Following raw materials according is weighed by the published proportioning of another patent of invention of our company:Wild Chinese silvergrass, wood Bits, wheat bran, corn flour, sucrose, land plaster, pulverized limestone;
(3)Spice, pack:The edible mushroom blender of the raw material weighed profession and sack filling machine are stirred and filled Bag, bacterium bag specification are Φ 15 ~ 25, and L25 ~ 45cm polypropylene plastics pockets, one is open, the high 15 ~ 25cm of material, material weight 1000g ± 1500g;
(4)Sterilizing:The bacterium bag installed is subjected to high pressure sterilization, temperature is 100 ~ 130 DEG C, 1.5 ~ 2.5h;
(5)Cooling:Bacterium bag after sterilizing is cooled to room temperature;
(6)Inoculation:Bacterium bag is sent into the inoculation indoor inoculation under hundred grades of purification conditions;Method is bacterium bag sterilizing cooling Afterwards, sack is opened, strain is put into bag, inoculum concentration is be equivalent to compost dry weight 3 ~ 8%;
(7)Cultural hypha:Culturing rack carries out cultural hypha, controlled at 20-28 DEG C;
(8)Go out sesame management:When mycelia covers with bacterium bag after a week, you can move into sesame room and be managed.
Sesame real estate management reason is as follows:
A) decylization lid, sack do not loosen, and front end is cut off with scissors, stay the sack of 3cm high;
B) mycelia is gone up 2 ~ 3 days, and temperature is 22-29 DEG C, is humidified to 85%, is ensured sack surface mycelia moistening, titanium dioxide Concentration of carbon is 1500-2500ppm, is protected from light culture;
C) flower bud, temperature maintain 22-29 DEG C, humidify to 90%-95%, and gas concentration lwevel is reduced to 600-1500ppm, The daily 600-1000 Lx of illumination;
D) after existing former base, temperature remains unchanged, and humidity is reduced to 85%-90%, and gas concentration lwevel is promoted to 1200- 1800ppm, the daily 600-1000 Lx of illumination;
E) former base is broken up, and temperature is continually maintained in 22-29 DEG C, and humidity maintains 80%-85%, and gas concentration lwevel is reduced to 600-1000ppm, the daily 800-1200 Lx of illumination;
(9)Harvesting:After going out sesame management 15-20 days, when ganoderma lucidum fruitbody cap no longer expands, cap edge is the same as cap color and luster Consistent spores flying can harvest.
Using the present invention, it on the one hand can make full use of and fail the renewable wild resource Chinese silvergrass developed completely now, greatly Reduction the forest reserves destruction;The ganoderma lucidum that another aspect is factory produced can prevent the invasion of pest and disease damage, improve product Quality reduces cost, improves cultivation benefit.
Specific embodiment:
The present invention is described in further detail below in conjunction with specific embodiment.
Embodiment 1
(1)Fermenting raw materials:Wild Chinese silvergrass and sawdust are crushed to average particle size as 10 mesh, after being irrigated with water, carried out respectively Heap fermentation is built, wild Chinese silvergrass fermentation time is 15 days minimum, sawdust fermentation time is used as ganoderma lucidum production medium major ingredient in 35 days;
(2)Raw material weighs:Raw material is weighed according to the following ratio:Wild Chinese silvergrass 50%, sawdust 20%, wheat bran 17%, corn flour 8%, Sucrose 1%, land plaster 1%, pulverized limestone 3%;
(3)Spice, pack:The edible mushroom blender of the raw material weighed profession and sack filling machine are stirred and filled Bag handles 4 baskets, and 12 bags every basket, bacterium bag specification is Φ 17*L36cm polypropylene plastics pockets, and an opening expects high 18cm, material weight 1175g±25g;
(4)Sterilizing:The bacterium bag installed is subjected to high pressure sterilization, temperature is 121 DEG C, 2.5h;
(5)Cooling:Bacterium bag after sterilizing is cooled to room temperature;
(6)Inoculation:Bacterium bag is sent into the inoculation indoor inoculation under hundred grades of purification conditions;Method is bacterium bag sterilizing cooling Afterwards, sack is opened, strain is put into bag, inoculum concentration is be equivalent to compost dry weight 5%;
(7)Cultural hypha:Cultural hypha is carried out with dedicated ganoderma lucidum culturing rack, controlled at 20 DEG C;
(8)Go out sesame management:Bacterium bag is covered with by the culture mycelia of 35 days, after a week, you can it moves into out sesame room and is managed, It is as follows:
A, decylization lid, sack do not loosen, and front end is cut off with scissors, stay the sack of 3cm high;
B, mycelia is gone up 3 days, and temperature is 22 DEG C, is humidified to 85%, is ensured sack surface mycelia moistening, gas concentration lwevel For 1500ppm, it is protected from light culture;
C, flower bud, temperature maintain 22 DEG C, humidify to 90%, gas concentration lwevel is reduced to 600ppm, illumination daily 600 Lx;
D, after existing former base, temperature remains unchanged, and humidity is reduced to 85%, and gas concentration lwevel is promoted to 1200ppm, illumination Daily 600Lx;
E, former base is broken up, and temperature is continually maintained in 22 DEG C, and humidity maintains 80%%, and gas concentration lwevel is reduced to 600ppm, Daily 800 Lx of illumination;
(9)Harvesting:After going out sesame management 22 days, when ganoderma lucidum fruitbody cap no longer expands, cap edge is the same as cap color and luster one Cause spores flying that can harvest;
(10)Every basket of total output is counted, and the shape of sample and (1 basket is taken out from 4 baskets) single bag of yield of statistics and fructification State shape (substance, cap length and width);
(11)Every basket of total output is respectively:A, 615g, B, 618g, C, 620g, D, 619g.Average total output is:618g.It takes out B samples count single bag of yield and form shapes, and single bag of equal yield is 51.5 g;The form of fructification:Ellipse;Substance 22-45 g; Cap length:9.0 -12.0cm;Cap width:6.0-9.0cm;
Embodiment 2
(1)Fermenting raw materials:Wild Chinese silvergrass and sawdust are crushed to average particle size as 15 mesh, after being irrigated with water, carried out respectively Heap fermentation is built, wild Chinese silvergrass fermentation time 15 days, sawdust fermentation time are used as ganoderma lucidum production medium major ingredient in 40 days;
(2)Raw material weighs:Raw material is weighed according to the following ratio:Wild Chinese silvergrass 50%, sawdust 20%, wheat bran 17%, corn flour 8%, Sucrose 1%, land plaster 1%, pulverized limestone 3%;.
(3)Spice, pack:The edible mushroom blender of the raw material weighed profession and sack filling machine are stirred and filled Bag handles 4 baskets, and 12 bags every basket, bacterium bag specification is Φ 17*36cm polypropylene plastics pockets, and an opening expects high 18cm, material weight 1175g±25g;
(4)Sterilizing:The bacterium bag installed is subjected to high pressure sterilization, temperature is 121 DEG C, 2.5h;
(5)Cooling:Bacterium bag after sterilizing is cooled to room temperature;
(6)Inoculation:Bacterium bag is sent into the inoculation indoor inoculation under hundred grades of purification conditions;Method is bacterium bag sterilizing cooling Afterwards, sack is opened, strain is put into bag, inoculum concentration is be equivalent to compost dry weight 7%;
(7)Cultural hypha:Cultural hypha is carried out with dedicated ganoderma lucidum culturing rack, controlled at 23 DEG C;
(8)Go out sesame management:Bacterium bag is covered with by the culture mycelia of 30 days, after a week, you can it moves into out sesame room and is managed, It is as follows:
A, decylization lid, sack do not loosen, and front end is cut off with scissors, stay the sack of 3cm high;
B, mycelia is gone up 3 days, and temperature is 25 DEG C, is humidified to 85%, is ensured sack surface mycelia moistening, gas concentration lwevel For 1500-2000ppm, it is protected from light culture;
C, flower bud, temperature maintain 25 DEG C, humidify to 90%%, gas concentration lwevel is reduced to 800ppm, illumination daily 800 Lx;
D, after existing former base, temperature remains unchanged, and humidity is reduced to 85%, and gas concentration lwevel is promoted to 1600ppm, illumination Daily 800 Lx;
E, former base is broken up, and temperature is continually maintained in 25 DEG C, and humidity maintains 85%, and gas concentration lwevel is reduced to 800ppm, light According to daily 1000 Lx;
(9)Harvesting:After going out sesame management 15-20 days, when ganoderma lucidum fruitbody cap no longer expands, cap edge is the same as cap color and luster Consistent spores flying can harvest;
(10)Every basket of total output is counted, and the shape of sample and (1 basket is taken out from 4 baskets) single bag of yield of statistics and fructification State shape (substance, cap length and width);
(11)Every basket of total output is respectively:A, 610g, B, 628g, C, 624g, D, 634g.Average total output is:624g.It takes out C samples count single bag of yield and form shapes, and single bag of equal yield is 52 g;The form of fructification:Ellipse;Substance 22-48 g;Bacterium Cover length:10.0 -13.5cm;Cap width:7.0-9.5cm.
Embodiment 3
(1)Fermenting raw materials:Wild Chinese silvergrass and sawdust are crushed to average particle size as 20 mesh, after being irrigated with water, carried out respectively Heap fermentation is built, wild Chinese silvergrass fermentation time 15 days, sawdust fermentation time are used as ganoderma lucidum production medium major ingredient in 45 days;
(2)Raw material weighs:Raw material is weighed according to the following ratio:Wild Chinese silvergrass 50%, sawdust 20%, wheat bran 17%, corn flour 8%, Sucrose 1%, land plaster 1%, pulverized limestone 3%;
(3)Spice, pack:The edible mushroom blender of the raw material weighed profession and sack filling machine are stirred and filled Bag handles 4 baskets, and 12 bags every basket, bacterium bag specification is 17*36cm polypropylene plastics pockets, and an opening expects high 18cm, expects weight 1175g ±25g;
(4)Sterilizing:The bacterium bag installed is subjected to high pressure sterilization, temperature is 130 DEG C, 2h;
(5)Cooling:Bacterium bag after sterilizing is cooled to room temperature;
(6)Inoculation:Bacterium bag is sent into the inoculation indoor inoculation under hundred grades of purification conditions;Method is bacterium bag sterilizing cooling Afterwards, sack is opened, strain is put into bag, inoculum concentration is be equivalent to compost dry weight 8%;
(7)Cultural hypha:Cultural hypha is carried out with dedicated ganoderma lucidum culturing rack, controlled at 28 DEG C;
(8)Go out sesame management:Bacterium bag is covered with by the culture mycelia of 28 days, after a week, you can it moves into out sesame room and is managed, It is as follows:
A, decylization lid, sack do not loosen, and front end is cut off with scissors, stay the sack of 3cm high;
B, mycelia is gone up 3 days, and temperature is 28 DEG C, is humidified to 85%, is ensured sack surface mycelia moistening, gas concentration lwevel For 2000ppm, it is protected from light culture;
C, flower bud, temperature maintain 28 DEG C, humidify to 95%, gas concentration lwevel is reduced to 1000ppm, illumination daily 1000 Lx;
D, after existing former base, temperature remains unchanged, and humidity is reduced to 90%, and gas concentration lwevel is promoted to 1800ppm, illumination Daily 1000 Lx;
E, former base is broken up, and temperature is continually maintained in 28 DEG C, and humidity maintains 85%, and gas concentration lwevel is reduced to 1000ppm, Daily 1200 Lx of illumination;
(9)Harvesting:After going out sesame management 14-19 days, when ganoderma lucidum fruitbody cap no longer expands, cap edge is the same as cap color and luster Consistent spores flying can harvest;
(10)Every basket of total output is counted, and the shape of sample and (1 basket is taken out from 4 baskets) single bag of yield of statistics and fructification State shape (substance, cap length and width);
(11)Every basket of total output is respectively:A, 588g, B, 590g, C, 598g, D, 600g.Average total output is:594g.It takes out A samples count single bag of yield and form shapes, and single bag of equal yield is 49 g;The form of fructification:Ellipse;Substance 21-45 g;Bacterium Cover length:10.0 -14.0cm;Cap width:7.0-10.0cm.

Claims (1)

  1. It is 1. a kind of using wild Chinese silvergrass as the method for major ingredient factory culture ganoderma lucidum, which is characterized in that include the following steps:
    (1)Fermenting raw materials:Wild Chinese silvergrass and sawdust are crushed to average particle size as 20 mesh, after being irrigated with water, carry out building heap respectively Fermentation, wild Chinese silvergrass fermentation time 15 days, sawdust fermentation time are used as ganoderma lucidum production medium major ingredient in 45 days;
    (2)Raw material weighs:Raw material is weighed according to the following ratio:Wild Chinese silvergrass 50%, sawdust 20%, wheat bran 17%, corn flour 8%, sucrose 1%, land plaster 1%, pulverized limestone 3%;
    (3)Spice, pack:The edible mushroom blender of the raw material weighed profession and sack filling machine are stirred and packed, is located 4 baskets of reason, 12 bags every basket, bacterium bag specification is 17*36cm polypropylene plastics pockets, and an opening expects high 18cm, expect weight 1175g ± 25g;
    (4)Sterilizing:The bacterium bag installed is subjected to high pressure sterilization, temperature is 130 DEG C, 2h;
    (5)Cooling:Bacterium bag after sterilizing is cooled to room temperature;
    (6)Inoculation:Bacterium bag is sent into the inoculation indoor inoculation under hundred grades of purification conditions;Method is after bacterium bag sterilizing cools down, to beat Strain is put into bag by opened mouth, and inoculum concentration is be equivalent to compost dry weight 8%;
    (7)Cultural hypha:Cultural hypha is carried out with dedicated ganoderma lucidum culturing rack, controlled at 28 DEG C;
    (8)Go out sesame management:Bacterium bag is covered with by the culture mycelia of 28 days, after a week, you can move into out sesame room and be managed, specifically Step is as follows:
    A, decylization lid, sack do not loosen, and front end is cut off with scissors, stay the sack of 3cm high;
    B, mycelia is gone up 3 days, and temperature is 28 DEG C, is humidified to 85%, is ensured sack surface mycelia moistening, gas concentration lwevel is 2000ppm is protected from light culture;
    C, flower bud, temperature maintain 28 DEG C, humidify to 95%, gas concentration lwevel is reduced to 1000ppm, the daily 1000Lx of illumination;
    D, after existing former base, temperature remains unchanged, and humidity is reduced to 90%, and gas concentration lwevel is promoted to 1800ppm, and illumination is daily 1000Lx;
    E, former base is broken up, and temperature is continually maintained in 28 DEG C, and humidity maintains 85%, and gas concentration lwevel is reduced to 1000ppm, illumination Daily 1200Lx;
    (9)Harvesting:After going out sesame management 14-19 days, when ganoderma lucidum fruitbody cap no longer expands, cap edge is consistent with cap color and luster Spores flying can harvest.
CN201510534238.7A 2015-08-28 2015-08-28 It is a kind of using wild Chinese silvergrass as the method for major ingredient factory culture ganoderma lucidum Expired - Fee Related CN105210669B (en)

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CN106518353A (en) * 2016-10-27 2017-03-22 广东雄达实业发展有限公司 Lucid ganoderma culture medium and preparation method thereof and lucid ganoderma planting method
CN106495806A (en) * 2016-10-27 2017-03-15 广东雄达实业发展有限公司 A kind of implantation methods of Medium for Ganoderma lucidum, preparation method and glossy ganoderma
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