CN106518353A - Lucid ganoderma culture medium and preparation method thereof and lucid ganoderma planting method - Google Patents
Lucid ganoderma culture medium and preparation method thereof and lucid ganoderma planting method Download PDFInfo
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- CN106518353A CN106518353A CN201610955329.2A CN201610955329A CN106518353A CN 106518353 A CN106518353 A CN 106518353A CN 201610955329 A CN201610955329 A CN 201610955329A CN 106518353 A CN106518353 A CN 106518353A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D3/00—Calcareous fertilisers
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Abstract
The invention discloses a lucid ganoderma culture medium and a preparation method thereof and a lucid ganoderma planting method. The culture medium is prepared from, by mass, 45%-55% of Chinese silvergrass, 35%-45% of chopped sawdust and 6%-14% of a mixture of wheat bran, corn flour, gypsum powder and brown sugar, wherein the contents of the wheat bran, the corn flour, the gypsum powder and the brown sugar are all higher than 0. The materials are proportioned and mixed according to the proportion, the mixed materials are contained in polypropylene or polyethylene film bags with the specification of 17 cm*33 cm*0.05 cm, 0.5-1.5 kg of the mixed materials are contained in each film bag, and lucid ganoderma is planted with the prepared culture medium. The lucid ganoderma produced through the method is high in nutrition content and productivity.
Description
Technical field
Fungus growing technique field of the present invention, especially a kind of Medium for Ganoderma lucidum and preparation method thereof.
Background technology
Ganoderma is the famous and precious kind in medicinal and edible health funguses, is sealed as Herba mesonae chinensis since ancient times.Modern medicine confirmation,
Take Ganoderma and can improve immunity of organisms, reach the effect prevented and cured diseases.It is for meeting wilderness demand of the people to Ganoderma, modern big
Amount culture Ganoderma by the way of artificial.The compost of prior art culture Ganoderma mainly has basswood, wood flour, cotton seed hullss to be equipped with wheat
Bran, analysis for soybean powder, Gypsum Fibrosum powder etc..Use basswood.Wood flour culture Ganoderma will consume the forest reserves in a large number；Although with cotton seed hullss culture Ganoderma
It is the recycling of agricultural byproducts, but cotton seed hullss there are pesticide residues, which utilizes and is subject to certain restrictions.Additionally, Medium for Ganoderma lucidum
Build environment require higher, need one to pollute the culture that extremely low Environment space can just smoothly complete culture medium.
The content of the invention
The technical problem to be solved in the present invention is to provide a kind of Medium for Ganoderma lucidum and preparation method thereof, and is based on the culture
The Ganoderma implantation methods of base, the ganoderma lucidum content that plantation is obtained are higher, and yield is bigger.
In order to solve above-mentioned technical problem, the present invention takes technical scheme below：
A kind of Medium for Ganoderma lucidum, the culture medium are made up of the following raw material according to mass percent meter：Miscanthus 45%～
55%, weed tree sawdust 35%～45%, and the mixture 6%～14% of Testa Tritici, Semen Maydis powder, Gypsum Fibrosum powder and remelt sugar.
The content of the Testa Tritici, Semen Maydis powder, Gypsum Fibrosum powder and remelt sugar is all higher than 0.
The culture medium is made up of the following raw material according to mass percent meter：Miscanthus 50%, weed tree sawdust 40%, Yi Jimai
The mixture 10% of skin, Semen Maydis powder, Gypsum Fibrosum powder and remelt sugar.
A kind of preparation method of Medium for Ganoderma lucidum, comprises the following steps：
The mixture of Miscanthus, weed tree sawdust and Testa Tritici, Semen Maydis powder, Gypsum Fibrosum powder and remelt sugar is mixed according to ratio
Uniformly, then carry out crushing to form powder and be uniformly mixing to obtain mixed dry material；
Pack, mixed dry material is loaded in thin film poly-bag, and the bag mouth of the thin film poly-bag is tightened, and in the bag fills in cotton
Flower；
Thin film poly-bag to installing mixed dry material carries out disinfection sterilization treatment.
The polypropylene or polythene film bag of the poly-bag preferably 17 centimetres of * 33 centimetres of * 0.05 centimetre of specifications, wherein 17 lis
Width of the rice for the poly-bag, 33 centimetres of height for the poly-bag, 0.05 centimetre of thickness for the poly-bag.
The quality of the mixed dry material loaded in the poly-bag is 0.5～1.5kg.
The bag mouth of the poly-bag is tightened using rope or lasso banding.
In the sterilization processing procedure, the poly-bag for installing mixed dry material is put in atmospheric sterilizing stove, 100 DEG C with
On temperature under kept for 20～25 hours, then vexed stove 4～6 hours naturally, complete the preparation of culture medium.
A kind of implantation methods of base Ganoderma, comprise the following steps：
Inoculation, culture medium is put to aseptic inoculation room, aseptically Ganoderma mycelium is seeded in culture medium；
Bacterium is sent out, postvaccinal culture medium is emitted on the indoor bedstead of culture, culture medium is completely covered in Ganoderma mycelium
In stage before charge level, indoor temperature control is cultivated at 22～25 DEG C；When Ganoderma mycelium be completely covered seal culture charge level it
Afterwards, by culture indoor temperature control at 25～28 DEG C, humid control is 60%～70%；
When there is overshooting shape former base and swell 1.5～1.6 centimetres to poly-bag bag mouth, and the Ganderma lucidum of culture medium charge level
When the Ganoderma mycelium portions turn color and fibrosiss of the bag wall of silk and poly-bag, poly-bag bag opening part is cut off, retain diameter
For 2～3 centimetres of passage, now Ganoderma sends out bacterium and completes, and the Medium for Ganoderma lucidum for sending out well bacterium is moved into cultivation in booth；
During the Medium for Ganoderma lucidum that bacterium is sent out in cultivation good in the booth, trench digging is carried out to cultivation area first and does furrow and sterilization, so
Afterwards Medium for Ganoderma lucidum is put in ditch and soil is covered；
When growing in the booth, the light brown of the white when the cap of Ganoderma is by initial growth to growth stage and growth
During the brown for completing, the air humidity in booth is kept 85～95%；
Go out the sesame stage in Ganoderma, keep the temperature in booth at 25～28 DEG C, humidity 90～95% often uses day ultrasonic fog
Change humidifier humidifies 4～6 times, each humidification periods are 30 minutes, complete the plantation of Ganoderma.
During described bacterium, when the temperature of culturing room is higher than 22 DEG C, daily to cultivating chamber ventilated twice, divulge information every time
Time is 10～12 hours；When the temperature of culturing room is less than 22 DEG C, every other day to cultivating chamber ventilated once, ventilation time is
2～3 hours.
The Ganoderma that the inventive method is obtained, nutrient content are higher, and yield is bigger.
Specific embodiment
For the ease of the understanding of those skilled in the art, the present invention is further retouched with reference to specific embodiment
State.
Embodiment one
Step 1, selects the raw material of following ratio according to mass percent：Miscanthus 50%, weed tree sawdust 40%, and Testa Tritici,
The mixture 10% of Semen Maydis powder, Gypsum Fibrosum powder and remelt sugar.The content of Testa Tritici, Semen Maydis powder, Gypsum Fibrosum powder and remelt sugar is all higher than 0, and protects
In the case where summation is 10%, the content of Testa Tritici, Semen Maydis powder, Gypsum Fibrosum powder and remelt sugar flexibly arbitrarily can be selected card.Will be upper
The raw material mix homogeneously of proportioning is stated, crushing is then carried out and is formed powder and be uniformly mixing to obtain mixed dry material.
Step 2, pack load mixed dry material in thin film poly-bag, and the bag mouth of the thin film poly-bag is tightened, and in the bag
Fill in Cotton Gossypii.The polypropylene or polythene film bag of poly-bag preferably 17 centimetres of * 33 centimetres of * 0.05 centimetre of specifications, wherein 17 centimetres
For the width of the poly-bag, 33 centimetres of height for the poly-bag, 0.05 centimetre of thickness for the poly-bag.The mixing loaded in poly-bag is done
The quality of material is 0.5kg.Wherein, the bag mouth of poly-bag is tightened using rope or lasso banding, convenient to operate.
Step 3, the poly-bag to installing mixed dry material carry out disinfection sterilization treatment.The poly-bag for installing mixed dry material is put into often
In pressure sterilizing kitchen, 23 hours are kept at a temperature of 100 DEG C, then vexed stove 6 hours naturally, complete the preparation of culture medium, obtain
Medium for Ganoderma lucidum.
The Medium for Ganoderma lucidum obtained through above-mentioned culture is carried out Ganderma lucidum as matrix by a kind of implantation methods of base Ganoderma
The growth planted, specifically includes following steps：
S1, inoculation, culture medium is put to aseptic inoculation room, Ganoderma mycelium is seeded in culture medium.Generally will culture
Base maintains less than 30 DEG C of temperature and carries out inoculation operation.
S2, sends out bacterium, postvaccinal culture medium is emitted on the indoor bedstead of culture, culture is completely covered in Ganoderma mycelium
In stage before base material face, indoor temperature control is cultivated at 22 DEG C, it is to avoid Ganoderma mycelium is dirty by miscellaneous bacteria at a higher temperature
Dye；After Ganoderma mycelium is completely covered and seals culture charge level, the indoor temperature of culture is promoted to into 25 DEG C from 22 DEG C before,
Humid control is 60%.When the temperature of culturing room is higher than 22 DEG C, daily to cultivating chamber ventilated twice, each ventilation time is 10
Hour；When the temperature of culturing room is less than 22 DEG C, every other day to cultivating chamber ventilated once, ventilation time is 2 hours.In ventilation
During, in addition it is also necessary to the big ups and downs of temperature are avoided, the low photoenvironment to culturing room is kept.It is close for putting for culture medium
Degree needs to leave suitable space, it is ensured that the circulation and the growth of mycelia of air.After being generally inoculated with 10 days, Ganoderma mycelium can be walked
The charge level of full Medium for Ganoderma lucidum, and deeply grow in the material of the Medium for Ganoderma lucidum, fruit body primordium can be formed within 30～35 days.
S3, after Ganoderma mycelium walks full culture medium charge level, it may appear that white projection, when the white overshooting shape former base and
1.52 centimetres are swelled to poly-bag bag mouth, and the Ganoderma mycelium portions turn of the bag wall of the Ganoderma mycelium and poly-bag of culture medium charge level
When color and fibrosiss, poly-bag bag opening part is cut off, retains a diameter of 2 centimetres of passage, now Ganoderma sends out bacterium and completes,
The Medium for Ganoderma lucidum for sending out bacterium good is moved in booth and is cultivated.
S4, during the Medium for Ganoderma lucidum that bacterium is sent out in cultivation good in the booth, first carries out trench digging to cultivation area and does furrow and sterilization,
Then Medium for Ganoderma lucidum is put in ditch and covers soil.During sterilization, can be carried out disinfection with water dissolution using potassium permanganate, or
After person other disinfectant water dissolutioies, sterilization is realized in sprinkling.
For booth place place, need to ensure that place surrounding is substantially free of contaminants.Water quality reaches requirement, especially
In water, heavy metal or pesticide can not all play standard.
S5, when growing in the booth, the light brown of the white when the cap of Ganoderma is by initial growth to growth stage and
During the brown that completes of growth, air humidity in booth is kept 85%.Medium for Ganoderma lucidum is buried after soil, needs to carry out
Moisturizing and ventilation, and avoid sunlight from shining directly in culture medium.According to sesame density is gone out, can suitably remove some connecting handles
Sesame, mutually extrudes after being grown up with bacteria-proof cover, affects quality.Generally stem grows cap, the bacterium successively Jing after the growth of 13 days
The color of lid progressively switchs to light brown by white, and then deepens into brown.Temperature control in canopy is at 25 DEG C.
S6, goes out the sesame stage in Ganoderma, keeps the temperature in booth at 25 DEG C, and humidity 90% is often added with day ultrasonic atomization
Wet device is humidified 4 times, and each humidification periods are 30 minutes, complete the plantation of Ganoderma.
Then Ganoderma sporophore Later growth will be into the spores release stage, and in good time carries out bagging to cap.The set
During the bag time should select the white edge of Ganoderma sporophore to be wholly absent afterwards, bag made of paper is generally used, prevents spore powder outside
Disperse.Now indoor temperature is normally controlled in 24 DEG C, air humidity 85%, and in good time ventilation, keeps the new of room air
It is fresh, prevent gas concentration lwevel from increasing.
Embodiment two
Step 1, selects the raw material of following ratio according to mass percent：Miscanthus 45%, weed tree sawdust 41%, and Testa Tritici,
The mixture 14% of Semen Maydis powder, Gypsum Fibrosum powder and remelt sugar.The content of Testa Tritici, Semen Maydis powder, Gypsum Fibrosum powder and remelt sugar is all higher than 0, and protects
In the case where summation is 14%, the content of Testa Tritici, Semen Maydis powder, Gypsum Fibrosum powder and remelt sugar flexibly arbitrarily can be selected card.Will be upper
The raw material mix homogeneously of proportioning is stated, crushing is then carried out and is formed powder and be uniformly mixing to obtain mixed dry material.
Step 2, pack load mixed dry material in thin film poly-bag, and the bag mouth of the thin film poly-bag is tightened, and in the bag
Fill in Cotton Gossypii.The polypropylene or polythene film bag of poly-bag preferably 17 centimetres of * 33 centimetres of * 0.05 centimetre of specifications, wherein 17 centimetres
For the width of the poly-bag, 33 centimetres of height for the poly-bag, 0.05 centimetre of thickness for the poly-bag.The mixing loaded in poly-bag is done
The quality of material is 1kg.Wherein, the bag mouth of poly-bag is tightened using rope or lasso banding, convenient to operate.
Step 3, the poly-bag to installing mixed dry material carry out disinfection sterilization treatment.The poly-bag for installing mixed dry material is put into often
In pressure sterilizing kitchen, 22 hours are kept at a temperature of 110 DEG C, then vexed stove 5 hours naturally, complete the preparation of culture medium, obtain
Medium for Ganoderma lucidum.
The Medium for Ganoderma lucidum obtained through above-mentioned culture is carried out Ganderma lucidum as matrix by a kind of implantation methods of base Ganoderma
The growth planted, specifically includes following steps：
S1, inoculation, culture medium is put to aseptic inoculation room, Ganoderma mycelium is seeded in culture medium.Generally will culture
Base maintains less than 30 DEG C of temperature and carries out inoculation operation.
S2, sends out bacterium, postvaccinal culture medium is emitted on the indoor bedstead of culture, culture is completely covered in Ganoderma mycelium
In stage before base material face, indoor temperature control is cultivated at 24 DEG C, it is to avoid Ganoderma mycelium is dirty by miscellaneous bacteria at a higher temperature
Dye；After Ganoderma mycelium is completely covered and seals culture charge level, the indoor temperature of culture is promoted to into 27 DEG C from 24 DEG C before,
Humid control is 65%.When the temperature of culturing room is higher than 22 DEG C, daily to cultivating chamber ventilated twice, each ventilation time is 11
Hour；When the temperature of culturing room is less than 22 DEG C, every other day to cultivating chamber ventilated once, ventilation time is 2 hours.In ventilation
During, in addition it is also necessary to the big ups and downs of temperature are avoided, the low photoenvironment to culturing room is kept.It is close for putting for culture medium
Degree needs to leave suitable space, it is ensured that the circulation and the growth of mycelia of air.After being generally inoculated with 10 days, Ganoderma mycelium can be walked
The charge level of full Medium for Ganoderma lucidum, and deeply grow in the material of the Medium for Ganoderma lucidum, fruit body primordium can be formed within 30～35 days.
S3, after Ganoderma mycelium walks full culture medium charge level, it may appear that white projection, when the white overshooting shape former base and
1.55 centimetres are swelled to poly-bag bag mouth, and the Ganoderma mycelium portions turn of the bag wall of the Ganoderma mycelium and poly-bag of culture medium charge level
When color and fibrosiss, poly-bag bag opening part is cut off, retains a diameter of 3 centimetres of passage, now Ganoderma sends out bacterium and completes,
The Medium for Ganoderma lucidum for sending out bacterium good is moved in booth and is cultivated.
S4, during the Medium for Ganoderma lucidum that bacterium is sent out in cultivation good in the booth, first carries out trench digging to cultivation area and does furrow and sterilization,
Then Medium for Ganoderma lucidum is put in ditch and covers soil.During sterilization, can be carried out disinfection with water dissolution using potassium permanganate, or
After person other disinfectant water dissolutioies, sterilization is realized in sprinkling.
For booth place place, need to ensure that place surrounding is substantially free of contaminants.Water quality reaches requirement, especially
In water, heavy metal or pesticide can not all play standard.
S5, when growing in the booth, the light brown of the white when the cap of Ganoderma is by initial growth to growth stage and
During the brown that completes of growth, air humidity in booth is kept 90%.Medium for Ganoderma lucidum is buried after soil, needs to carry out
Moisturizing and ventilation, and avoid sunlight from shining directly in culture medium.According to sesame density is gone out, can suitably remove some connecting handles
Sesame, mutually extrudes after being grown up with bacteria-proof cover, affects quality.Generally stem grows cap, the bacterium successively Jing after the growth of 13 days
The color of lid progressively switchs to light brown by white, and then deepens into brown.Temperature control in canopy is at 28 DEG C.
S6, goes out the sesame stage in Ganoderma, keeps the temperature in booth at 27 DEG C, and humidity 93% is often added with day ultrasonic atomization
Wet device is humidified 5 times, and each humidification periods are 30 minutes, complete the plantation of Ganoderma.
Then Ganoderma sporophore Later growth will be into the spores release stage, and in good time carries out bagging to cap.The set
During the bag time should select the white edge of Ganoderma sporophore to be wholly absent afterwards, bag made of paper is generally used, prevents spore powder outside
Disperse.Now indoor temperature is normally controlled in 24 DEG C, air humidity 85%, and in good time ventilation, keeps the new of room air
It is fresh, prevent gas concentration lwevel from increasing.
After Ganoderma full maturity, it is possible to carry out harvesting work.Generally forming harvesting from cap about needs 20 days, lid bacterium
The color at lid edge switchs to brown by white, when the spore powder of coffee color is indistinctly seen at the cap back side by harvested.First
Criticize after harvesting is finished and sterilized once, be separated by 7 days and spray water afterwards again, make Ganoderma mycelium restoration ecosystem, promote new Ganderma lucidum
Flower bud is formed, and is generally separated by the harvesting for being capable of achieving second batch Ganoderma for 2 months.
Embodiment three
Step 1, selects the raw material of following ratio according to mass percent：Miscanthus 55%, weed tree sawdust 35%, and Testa Tritici,
The mixture 10% of Semen Maydis powder, Gypsum Fibrosum powder and remelt sugar.The content of Testa Tritici, Semen Maydis powder, Gypsum Fibrosum powder and remelt sugar is all higher than 0, and protects
In the case where summation is 10%, the content of Testa Tritici, Semen Maydis powder, Gypsum Fibrosum powder and remelt sugar flexibly arbitrarily can be selected card.Will be upper
The raw material mix homogeneously of proportioning is stated, crushing is then carried out and is formed powder and be uniformly mixing to obtain mixed dry material.
Step 2, pack load mixed dry material in thin film poly-bag, and the bag mouth of the thin film poly-bag is tightened, and in the bag
Fill in Cotton Gossypii.The polypropylene or polythene film bag of poly-bag preferably 17 centimetres of * 33 centimetres of * 0.05 centimetre of specifications, wherein 17 centimetres
For the width of the poly-bag, 33 centimetres of height for the poly-bag, 0.05 centimetre of thickness for the poly-bag.The mixing loaded in poly-bag is done
The quality of material is 1.5kg.Wherein, the bag mouth of poly-bag is tightened using rope or lasso banding, convenient to operate.
Step 3, the poly-bag to installing mixed dry material carry out disinfection sterilization treatment.The poly-bag for installing mixed dry material is put into often
In pressure sterilizing kitchen, 20 hours are kept at a temperature of 120 DEG C, then vexed stove 4 hours naturally, complete the preparation of culture medium, obtain
Medium for Ganoderma lucidum.
The Medium for Ganoderma lucidum obtained through above-mentioned culture is carried out Ganderma lucidum as matrix by a kind of implantation methods of base Ganoderma
The growth planted, specifically includes following steps：
S1, inoculation, culture medium is put to aseptic inoculation room, Ganoderma mycelium is seeded in culture medium.Generally will culture
Base maintains less than 30 DEG C of temperature and carries out inoculation operation.
S2, sends out bacterium, postvaccinal culture medium is emitted on the indoor bedstead of culture, culture is completely covered in Ganoderma mycelium
In stage before base material face, indoor temperature control is cultivated at 25 DEG C, it is to avoid Ganoderma mycelium is dirty by miscellaneous bacteria at a higher temperature
Dye；After Ganoderma mycelium is completely covered and seals culture charge level, the indoor temperature of culture is promoted to into 28 DEG C from 25 DEG C before,
Humid control is 70%.When the temperature of culturing room is higher than 22 DEG C, daily to cultivating chamber ventilated twice, each ventilation time is 12
Hour；When the temperature of culturing room is less than 22 DEG C, every other day to cultivating chamber ventilated once, ventilation time is 2 hours.In ventilation
During, in addition it is also necessary to the big ups and downs of temperature are avoided, the low photoenvironment to culturing room is kept.It is close for putting for culture medium
Degree needs to leave suitable space, it is ensured that the circulation and the growth of mycelia of air.After being generally inoculated with 10 days, Ganoderma mycelium can be walked
The charge level of full Medium for Ganoderma lucidum, and deeply grow in the material of the Medium for Ganoderma lucidum, fruit body primordium can be formed within 30～35 days.
S3, after Ganoderma mycelium walks full culture medium charge level, it may appear that white projection, when the white overshooting shape former base and
1.6 centimetres are swelled to poly-bag bag mouth, and the Ganoderma mycelium portions turn of the bag wall of the Ganoderma mycelium and poly-bag of culture medium charge level
When color and fibrosiss, poly-bag bag opening part is cut off, retains a diameter of 2 centimetres of passage, now Ganoderma sends out bacterium and completes,
The Medium for Ganoderma lucidum for sending out bacterium good is moved in booth and is cultivated.
S4, during the Medium for Ganoderma lucidum that bacterium is sent out in cultivation good in the booth, first carries out trench digging to cultivation area and does furrow and sterilization,
Then Medium for Ganoderma lucidum is put in ditch and covers soil.During sterilization, can be carried out disinfection with water dissolution using potassium permanganate, or
After person other disinfectant water dissolutioies, sterilization is realized in sprinkling.
For booth place place, need to ensure that place surrounding is substantially free of contaminants.Water quality reaches requirement, especially
In water, heavy metal or pesticide can not all play standard.
S5, when growing in the booth, the light brown of the white when the cap of Ganoderma is by initial growth to growth stage and
During the brown that completes of growth, air humidity in booth is kept 85%.Medium for Ganoderma lucidum is buried after soil, needs to carry out
Moisturizing and ventilation, and avoid sunlight from shining directly in culture medium.According to sesame density is gone out, can suitably remove some connecting handles
Sesame, mutually extrudes after being grown up with bacteria-proof cover, affects quality.Generally stem grows cap, the bacterium successively Jing after the growth of 13 days
The color of lid progressively switchs to light brown by white, and then deepens into brown.Temperature control in canopy is at 25 DEG C.
S6, goes out the sesame stage in Ganoderma, keeps the temperature in booth at 28 DEG C, and humidity 95% is often added with day ultrasonic atomization
Wet device is humidified 6 times, and each humidification periods are 30 minutes, complete the plantation of Ganoderma.
Then Ganoderma sporophore Later growth will be into the spores release stage, and in good time carries out bagging to cap.The set
During the bag time should select the white edge of Ganoderma sporophore to be wholly absent afterwards, bag made of paper is generally used, prevents spore powder outside
Disperse.Now indoor temperature is normally controlled in 24 DEG C, air humidity 85%, and in good time ventilation, keeps the new of room air
It is fresh, prevent gas concentration lwevel from increasing.
It should be noted that the above is not limited to the present invention, in the creation design without departing from the present invention
Under the premise of, any obvious replacement is within protection scope of the present invention.
Claims (10)
1. a kind of Medium for Ganoderma lucidum, it is characterised in that the culture medium is made up of the following raw material according to mass percent meter：Awns
Grass 45%～55%, weed tree sawdust 35%～45%, and the mixture 6%～14% of Testa Tritici, Semen Maydis powder, Gypsum Fibrosum powder and remelt sugar.
2. Medium for Ganoderma lucidum according to claim 1, it is characterised in that the Testa Tritici, Semen Maydis powder, Gypsum Fibrosum powder and remelt sugar
Content is all higher than 0.
3. Medium for Ganoderma lucidum according to claim 2, it is characterised in that the culture medium is by according to mass percent meter
Following raw material is constituted：The mixture 10% of Miscanthus 50%, weed tree sawdust 40%, and Testa Tritici, Semen Maydis powder, Gypsum Fibrosum powder and remelt sugar.
4. a kind of preparation method of the Medium for Ganoderma lucidum according to claim 1 or 2 or 3, it is characterised in that including following step
Suddenly：
The mixture of Miscanthus, weed tree sawdust and Testa Tritici, Semen Maydis powder, Gypsum Fibrosum powder and remelt sugar is carried out mixing according to ratio
It is even, then carry out crushing and form powder and be uniformly mixing to obtain mixed dry material；
Pack, mixed dry material is loaded in thin film poly-bag, and the bag mouth of the thin film poly-bag is tightened, and in the bag fills in Cotton Gossypii；
Thin film poly-bag to installing mixed dry material carries out disinfection sterilization treatment.
5. the preparation method of Medium for Ganoderma lucidum according to claim 4, it is characterised in that preferably 17 centimetres * of the poly-bag
The polypropylene or polythene film bag of 33 centimetres of * 0.05 centimetre of specifications, the wherein 17 centimetres width for the poly-bag, 33 centimetres is to be somebody's turn to do
The height of poly-bag, 0.05 centimetre of thickness for the poly-bag.
6. the preparation method of Medium for Ganoderma lucidum according to claim 5, it is characterised in that the mixing loaded in the poly-bag
The quality of siccative is 0.5～1.5kg.
7. the preparation method of Medium for Ganoderma lucidum according to claim 6, it is characterised in that the bag mouth of the poly-bag is using rope
Son is tightened or lasso banding.
8. the preparation method of Medium for Ganoderma lucidum according to claim 7, it is characterised in that the sterilization processing procedure
In, the poly-bag for installing mixed dry material is put in atmospheric sterilizing stove, more than 100 DEG C at a temperature of keep 20～25 hours, so
Naturally vexed stove 4～6 hours, complete the preparation of culture medium afterwards.
9. a kind of implantation methods of the Ganoderma of the Medium for Ganoderma lucidum based on according to any one of claim 1～8, including with
Lower step：
Inoculation, culture medium is put to aseptic inoculation room, aseptically Ganoderma mycelium is seeded in culture medium；
Bacterium is sent out, postvaccinal culture medium is emitted on the indoor bedstead of culture, culture medium charge level is completely covered in Ganoderma mycelium
In the stage before, indoor temperature control is cultivated at 22～25 DEG C；After Ganoderma mycelium is completely covered and seals culture charge level, will
At 25～28 DEG C, humid control is 60%～70% for the indoor temperature control of culture；
When occur overshooting shape former base and to poly-bag bag mouth swell 1.5～1.6 centimetres, and the Ganoderma mycelium of culture medium charge level and
When the Ganoderma mycelium portions turn color and fibrosiss of the bag wall of poly-bag, poly-bag bag opening part is cut off, retain it is a diameter of 2～
3 centimetres of passage, now Ganoderma send out bacterium and complete, the Medium for Ganoderma lucidum for sending out well bacterium is moved into into cultivation in booth；
During the Medium for Ganoderma lucidum that bacterium is sent out well in cultivation in the booth, trench digging is carried out to cultivation area first and does furrow and sterilization, then general
During Medium for Ganoderma lucidum is put into ditch and cover soil；
When growing in the booth, the light brown of the white when the cap of Ganoderma is by initial growth to growth stage and growth are completed
Brown during, keep the air humidity in booth 85～95%；
Go out the sesame stage in Ganoderma, keep the temperature in booth at 25～28 DEG C, humidity 90～95% is often added with day ultrasonic atomization
Wet device is humidified 4～6 times, and each humidification periods are 30 minutes, complete the plantation of Ganoderma.
10. implantation methods of Ganoderma according to claim 9, it is characterised in that during described bacterium, when culturing room
When temperature is higher than 22 DEG C, daily to cultivating chamber ventilated twice, each ventilation time is 10～12 hours；When the temperature of culturing room it is low
When 22 DEG C, every other day to cultivating chamber ventilated once, ventilation time is 2～3 hours.
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| CN107873394A (en) * | 2017-12-05 | 2018-04-06 | 罗成喜 | A kind of implantation methods of ganoderma lucidum |
| CN108901629A (en) * | 2018-07-03 | 2018-11-30 | 辽宁省林业科学研究院 | A kind of lucid ganoderma stock culture cultural method, culture medium and culture medium preparation method |
| CN108934764A (en) * | 2018-08-16 | 2018-12-07 | 册亨县布依酒业有限公司 | A kind of implantation methods of semi-wild Black Ganoderma |
| CN112493054A (en) * | 2020-11-23 | 2021-03-16 | 安徽农业大学 | Ganoderma lucidum culture material based on moso bamboo sawdust and preparation method and application thereof |
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| CN108934764A (en) * | 2018-08-16 | 2018-12-07 | 册亨县布依酒业有限公司 | A kind of implantation methods of semi-wild Black Ganoderma |
| CN112493054A (en) * | 2020-11-23 | 2021-03-16 | 安徽农业大学 | Ganoderma lucidum culture material based on moso bamboo sawdust and preparation method and application thereof |
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