CN104641941A - Method for cultivating white fungus on mulberry twigs - Google Patents
Method for cultivating white fungus on mulberry twigs Download PDFInfo
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- CN104641941A CN104641941A CN201510108015.4A CN201510108015A CN104641941A CN 104641941 A CN104641941 A CN 104641941A CN 201510108015 A CN201510108015 A CN 201510108015A CN 104641941 A CN104641941 A CN 104641941A
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- dry powder
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- ramulus mori
- uricularia polytricha
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- 238000000034 method Methods 0.000 title claims abstract description 33
- 240000000249 Morus alba Species 0.000 title claims abstract description 12
- 235000008708 Morus alba Nutrition 0.000 title claims abstract description 12
- 241000233866 Fungi Species 0.000 title abstract description 10
- 239000000843 powder Substances 0.000 claims abstract description 87
- 241000894006 Bacteria Species 0.000 claims abstract description 47
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims abstract description 26
- 240000002234 Allium sativum Species 0.000 claims abstract description 22
- 244000037364 Cinnamomum aromaticum Species 0.000 claims abstract description 22
- 235000004611 garlic Nutrition 0.000 claims abstract description 22
- 235000013399 edible fruits Nutrition 0.000 claims abstract description 16
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims abstract description 13
- 235000019341 magnesium sulphate Nutrition 0.000 claims abstract description 13
- 230000001954 sterilising effect Effects 0.000 claims abstract description 11
- 239000001963 growth medium Substances 0.000 claims abstract description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 48
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 23
- 238000011081 inoculation Methods 0.000 claims description 22
- 239000002609 medium Substances 0.000 claims description 22
- 235000014489 Cinnamomum aromaticum Nutrition 0.000 claims description 21
- 241000736078 Schisandra sphenanthera Species 0.000 claims description 17
- 229920000742 Cotton Polymers 0.000 claims description 13
- 235000015099 wheat brans Nutrition 0.000 claims description 13
- PASHVRUKOFIRIK-UHFFFAOYSA-L calcium sulfate dihydrate Chemical compound O.O.[Ca+2].[O-]S([O-])(=O)=O PASHVRUKOFIRIK-UHFFFAOYSA-L 0.000 claims description 12
- 239000007921 spray Substances 0.000 claims description 12
- 239000004615 ingredient Substances 0.000 claims description 11
- YYRMJZQKEFZXMX-UHFFFAOYSA-N calcium;phosphoric acid Chemical compound [Ca+2].OP(O)(O)=O.OP(O)(O)=O YYRMJZQKEFZXMX-UHFFFAOYSA-N 0.000 claims description 10
- 239000000284 extract Substances 0.000 claims description 10
- 239000002426 superphosphate Substances 0.000 claims description 10
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 claims description 9
- 230000005070 ripening Effects 0.000 claims description 8
- 238000004519 manufacturing process Methods 0.000 claims description 7
- 238000001816 cooling Methods 0.000 claims description 6
- 238000004659 sterilization and disinfection Methods 0.000 claims description 6
- 239000000463 material Substances 0.000 claims description 5
- -1 polypropylene Polymers 0.000 claims description 5
- 238000002360 preparation method Methods 0.000 claims description 5
- 206010004542 Bezoar Diseases 0.000 claims description 4
- 210000004209 hair Anatomy 0.000 claims description 4
- 230000008569 process Effects 0.000 claims description 4
- 241001237732 Microtia Species 0.000 claims description 3
- 239000004743 Polypropylene Substances 0.000 claims description 3
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 3
- 238000002347 injection Methods 0.000 claims description 3
- 239000007924 injection Substances 0.000 claims description 3
- 238000002156 mixing Methods 0.000 claims description 3
- 230000003020 moisturizing effect Effects 0.000 claims description 3
- 229910052760 oxygen Inorganic materials 0.000 claims description 3
- 239000001301 oxygen Substances 0.000 claims description 3
- 238000006213 oxygenation reaction Methods 0.000 claims description 3
- 229920001155 polypropylene Polymers 0.000 claims description 3
- 238000005507 spraying Methods 0.000 claims description 3
- 230000006641 stabilisation Effects 0.000 claims description 3
- 238000011105 stabilization Methods 0.000 claims description 3
- 238000009423 ventilation Methods 0.000 claims description 3
- 238000002386 leaching Methods 0.000 claims description 2
- 241000238631 Hexapoda Species 0.000 abstract description 5
- 208000035240 Disease Resistance Diseases 0.000 abstract description 4
- 240000006079 Schisandra chinensis Species 0.000 abstract 1
- YYRMJZQKEFZXMX-UHFFFAOYSA-L calcium bis(dihydrogenphosphate) Chemical compound [Ca+2].OP(O)([O-])=O.OP(O)([O-])=O YYRMJZQKEFZXMX-UHFFFAOYSA-L 0.000 abstract 1
- 229910000389 calcium phosphate Inorganic materials 0.000 abstract 1
- 235000017803 cinnamon Nutrition 0.000 abstract 1
- 235000012343 cottonseed oil Nutrition 0.000 abstract 1
- 229910052602 gypsum Inorganic materials 0.000 abstract 1
- 239000010440 gypsum Substances 0.000 abstract 1
- 208000015181 infectious disease Diseases 0.000 abstract 1
- 230000002458 infectious effect Effects 0.000 abstract 1
- 244000005700 microbiome Species 0.000 abstract 1
- 235000019691 monocalcium phosphate Nutrition 0.000 abstract 1
- 238000007493 shaping process Methods 0.000 abstract 1
- 230000001580 bacterial effect Effects 0.000 description 11
- 230000009466 transformation Effects 0.000 description 8
- 241000607479 Yersinia pestis Species 0.000 description 6
- 230000000052 comparative effect Effects 0.000 description 6
- 239000003814 drug Substances 0.000 description 6
- 241001506047 Tremella Species 0.000 description 5
- 239000002994 raw material Substances 0.000 description 5
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 description 4
- 238000011109 contamination Methods 0.000 description 4
- 238000012258 culturing Methods 0.000 description 4
- 201000010099 disease Diseases 0.000 description 4
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 4
- 239000004033 plastic Substances 0.000 description 4
- 229920003023 plastic Polymers 0.000 description 4
- 235000001674 Agaricus brunnescens Nutrition 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 241000255789 Bombyx mori Species 0.000 description 2
- 239000004698 Polyethylene Substances 0.000 description 2
- 206010042928 Syringomyelia Diseases 0.000 description 2
- 238000009395 breeding Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 229910000019 calcium carbonate Inorganic materials 0.000 description 2
- 235000019994 cava Nutrition 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 239000003112 inhibitor Substances 0.000 description 2
- 239000002054 inoculum Substances 0.000 description 2
- 230000035800 maturation Effects 0.000 description 2
- 229920000573 polyethylene Polymers 0.000 description 2
- 230000037303 wrinkles Effects 0.000 description 2
- 235000000023 Auricularia auricula Nutrition 0.000 description 1
- 240000005710 Auricularia polytricha Species 0.000 description 1
- TWFZGCMQGLPBSX-UHFFFAOYSA-N Carbendazim Natural products C1=CC=C2NC(NC(=O)OC)=NC2=C1 TWFZGCMQGLPBSX-UHFFFAOYSA-N 0.000 description 1
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 241000223261 Trichoderma viride Species 0.000 description 1
- 239000003899 bactericide agent Substances 0.000 description 1
- 230000003385 bacteriostatic effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- JNPZQRQPIHJYNM-UHFFFAOYSA-N carbendazim Chemical compound C1=C[CH]C2=NC(NC(=O)OC)=NC2=C1 JNPZQRQPIHJYNM-UHFFFAOYSA-N 0.000 description 1
- 239000006013 carbendazim Substances 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000000084 colloidal system Substances 0.000 description 1
- 238000012364 cultivation method Methods 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 235000013312 flour Nutrition 0.000 description 1
- 235000020706 garlic extract Nutrition 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 229910052749 magnesium Inorganic materials 0.000 description 1
- 239000011777 magnesium Substances 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 238000011177 media preparation Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 235000008935 nutritious Nutrition 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 238000009366 sericulture Methods 0.000 description 1
- 230000004763 spore germination Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
- 230000001256 tonic effect Effects 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/40—Fertilisers incorporated into a matrix
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Mycology (AREA)
- Environmental Sciences (AREA)
- Mushroom Cultivation (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a method for cultivating white fungus on mulberry twigs. The method comprises the following steps: (a) preparing a culture medium; (b) burdening, bagging and sterilizing; (c) inoculating; (d) performing bacterium cultivation; (e) performing management at a growth and development period; (f) performing management at a white fungus shaping period. The formula of the culture medium comprises 10 parts of mulberry branch powder, 10 parts of Chinese magnoliavine fruit dry powder, 5 parts of garlic dry powder, 5 parts of Chinese cinnamon dry powder, 5 parts of poisonnut dry powder, 10 parts of bran, 10 parts of cottonseed hull, 1 part of sugar, 1 part of gypsum powder, 0.2 part of calcium superphosphate and 0.1 part of magnesium sulfate. By adopting the method for cultivating white fungus on mulberry twigs, the problems of a large amount of infectious microbes during cultivation of white fungus in southern areas is solved, the percentage of contaminating microorganism of the cultivated white fungus is lower, and higher insect resistance and disease resistance are achieved.
Description
Technical field
The present invention relates to a kind of agricultural product production technical field, be specifically related to a kind of method that ramulus mori cultivates white Uricularia polytricha.
Background technology
White Uricularia polytricha, is also white Uricularia polytricha, tremella, has the laudatory title of " hat in bacterium ".It is famous and precious nutritious tonifying good merchantable brand, is again the strong tonic of righting.Uricularia polytricha common are black flour and carries on the back in vain or the yellow back of the body, the two-sided different cultivars such as pink or purplish red.Its fruit body is soft, and the back side covers with thin short white-colored hairs, and the fresh white slightly micro-Huang of outward appearance, meat is transparent, the tender mouthfeel of cunning had close to woodear of food.White Uricularia polytricha fruit body colloid, shallow disc, ear form irregular shape, wide 2-15 μm.Have obvious base portion, stockless, base portion slightly wrinkles, soft time fresh, dry after-contraction.The raw the inside of sporiferous layer, smoothly or slightly wrinkle, white, rear change ecru.There is longer white fluff outside, colourless, only base portion brown, 400-1100 × 4.5-6.5 μm.Normal bunchy growth.
Warm higher type mushroom in white Uricularia polytricha.Mycelial growth temperature is 10-36 DEG C, the suitableeest 20-31 DEG C; Sporophore growth temperature is 18-33 DEG C, and optimum temperature is 20-25 DEG C, and lower than 18 DEG C, fruit body is not easily formed or grows and is suppressed; Higher than 35 DEG C, fruit body stops growing.
Existing white Uricularia polytricha culture technique roughly can be summarized as follows:
(1) white Uricularia polytricha cultivation season: this bacterial strain is to the requirement of temperature: mycelial growth 22-32 DEG C, sporophore growth 23-32 DEG C, cultivation season is suitable with inoculation spring, screech owl summer.
(2) selected by: cultivation place is relatively more extensive, can make full use of indoor, mushroom room, dish canopy, field, forest land.But total requirement is shaded and is wanted to establish 6-8 layer to arrange bag frame, floor height 24 centimetres in " two sun eight are cloudy " indoor growing room.
(3) medium preparation: weed tree sawdust 68%, cotton seed hulls 15%, wheat bran 15%, land plaster 1%, calcium carbonate 1%.Material and water ratio 1:1-1:2.
(4) sterilizing is packed: adopt low-pressure polyethylene plastics cultivation bag, pocket sterilizing more than 100 DEG C keeps 20-22 hour.
(5) bacterium is sent out in inoculation: 4 inoculation caves are made a call in every bag of front, and inoculum concentration is by the bacterial classification of 13.5 × 24 centimetres of gage plastic bags, and every bag connects 25-30 cultivation bag; Bacteria developing period temperature is to be not less than 22 DEG C and do not surpass 32 DEG C as well, and after 30 days, mycelia can walk purseful.
(6) go out syrinx reason: cultivated through 30 days after inoculation, mycelia physiological ripening can start bait operation, sets up earhole in bag side, relative moisture keeps 80%, until fruit body maturation is gathered.
But due to because white Uricularia polytricha belongs to the higher type mushroom of middle temperature, although the applicable white Uricularia polytricha growth of environment that middle temperature is higher, be more conducive to spore germination and the growth and breeding of the multiple moulds such as Trichoderma viride.If cultivate white Uricularia polytricha in south, certainly exist the problem that miscellaneous bacteria is grown thickly.
Certainly the method for artificial medicament can be adopted to suppress the growth of miscellaneous bacteria, such as the agriculturally bactericidal agent such as widely used carbendazim, certain good effect is all played to suppression varied bacteria growing.But, after adding artificial medicament, how much all can there is medicament residue, make white Uricularia polytricha not reach the requirement of pollution-free food.
Therefore be necessary very much to research and develop a kind of white Uricularia polytricha culture technique newly, improve the problem that white Uricularia polytricha cultivation miscellaneous bacteria is more better.
Summary of the invention
The object of the invention is to solve the more problem of white Uricularia polytricha cultivation miscellaneous bacteria, provide a kind of ramulus mori of varied bacteria growing that can significantly suppress and cultivate the method for white Uricularia polytricha.
Ramulus mori of the present invention cultivates the method for white Uricularia polytricha, comprises the following steps:
A) medium is made: culture medium prescription is as follows: ramulus mori bits 50-65 part, Schisandra sphenanthera Rehd.et WilS dry powder 10-20 part, garlic dry powder 5-10 part, cassia bark dry powder 5-10 part, wooden small pin for the case dry powder 5-10 part, wheat bran 10-20 part, cotton seed hulls 10-20 part, sugared 1-3 part, land plaster 1-3 part, superphosphate 0.2-0.7 part, magnesium sulfate 0.1-0.3 part;
Manufacturing process is as follows: take the ramulus mori bits of respective amount, Schisandra sphenanthera Rehd.et WilS dry powder, garlic dry powder, cassia bark dry powder, wooden small pin for the case dry powder, wheat bran, cotton seed hulls be mixed into major ingredient, pour into as the sugar of clout, land plaster, superphosphate, magnesium sulfate in major ingredient after being dissolved in a small amount of water again, turn to water content reach 50-60% while add clear water limit; Adjust the pH value of white Uricularia polytricha medium between 5.5-6.5;
B) batching pack sterilizing: the medium batching of step a) gained is loaded in pocket, obtained bacterium rod; To heat under bacterium rod is placed in condition of normal pressure sterilizing, keep 10-l2 hour when the temperature stabilization in bag is at 1O0 DEG C, then cooling naturally;
C) inoculate: beat 3-5 inoculation cave in pocket front, when bacterium rod material temperature drop is to less than 25 DEG C, white Uricularia polytricha original seed is inoculated in inoculation cave;
D) cultivation: just the bacterium rod of step c gained moves into outdoor booth, the parallel adjacent stacking of bacterium rod; Inoculate after 9-16 days, in the inoculation cave place enlarging oxygenation of pocket, make mycelia obtain sufficient oxygen; Utilize spraying cooling in conjunction with forced ventilation simultaneously, control canopy temperature at 20-25 DEG C, relative moisture 60-70%; 18-25 days after inoculation, former base starts to differentiate microtia bud, enters the young ear phase;
E) management of growth and development stage: long bacterium bag is moved to agaric growing field or agaric growing room, is built into wall type structure, temperature 20-25 DEG C, relative moisture 60-70%, when young ear length is to diameter about 12cm, sprays water in right amount to bacterium bag, makes its auricle moistening;
F) become the management of ear phase: when entity grows to diameter about 12cm, fruit body comes to the ripening period, now stop water spray, temperature maintains 22-24 DEG C, and entering picking time can gather after 6-8 days.
Further illustrate as of the present invention, in step a, described ramulus mori can be fresh mulberry bar, branch, also can be the ramulus mori parched; Wherein, fresh ramulus mori is directly applied, and the ramulus mori parched is pulled out after will being soaked in water 3-5 hour to drain away the water and just applied, and ensureing that ramulus mori considers water content to be worth doing is 6-10%.
Further illustrate as of the present invention, in step a, the preparation method of Schisandra sphenanthera Rehd.et WilS dry powder, garlic dry powder, cassia bark dry powder, wooden small pin for the case dry powder is as follows: get Chinese Magnolivine Fruit, garlic, cassia bark powder, wooden small pin for the case powder, respectively adopt volumetric concentration be 60-80% ethanol leaching 2-3 time, each 1-3 hour, obtain extract, more namely gained extract removing ethanol is obtained corresponding dry powder.
Further illustrate as of the present invention, in step b, medium batching is dispensed into rapidly in polypropylene bacterium bag after mixing, in order to avoid windrow is long for standing time, causes and becomes sour; Every bag approximately loads height 18-20cm.
Further illustrate as of the present invention, in step c, seeded process operates on aseptic operating platform, needs in advance to carry out ultraviolet disinfection 0.5-1h to operating desk, before inoculation, also to carry out disinfection to the hand medicinal alcohol of the table top in operating desk, article and operator.
Further illustrate as of the present invention, in steps d, the ceiling of described booth covers black and white film, arranges sunshade net above ceiling, erection spray equipment.
Further illustrate as of the present invention, in steps d, administration period is noted checking pollution condition, as miscellaneous bacteria appears in cave mouth, kills with formaldehyde injection.
Further illustrate as of the present invention, in step e, the method keeping auricle moistening covers newspaper on bacterium bag, then carry out water spray moisturizing.The object of lid newspaper: one is that water can not directly be sprayed onto on the mouth of cave and cause ponding; Two is make the little space between newspaper and bacterium rod have high humidity of air, unlikely make white hair ball dry up, be beneficial to ear base differentiation.
Further illustrate as of the present invention, in steps d, inoculate after 4 days, cave mouth engenders white projection fine hair shape hypha body, and now temperature should control at 20-23 DEG C, and relative moisture controls at 8O-85%; Along with mycelia physiological ripening, there is light yellow globule in white hair ball, now by cave mouth turning, yellow water should be flowed out outside cave, afterwards temperature is raised to about 25 DEG C, yellow water is shunk.
The method that ramulus mori of the present invention cultivates white Uricularia polytricha has following technological progress compared to existing technology:
(1) make in medium process, be mixed into major ingredient with ramulus mori bits, Schisandra sphenanthera Rehd.et WilS dry powder, garlic dry powder, cassia bark dry powder, wooden small pin for the case dry powder, wheat bran, cotton seed hulls.Wherein the dry powder such as Schisandra sphenanthera Rehd.et WilS, garlic extracts from the plant of correspondence, containing natural bacteriostatic composition.The present invention by four kinds of Chinese medicine dry powder with the use of, make the synergy between them, not only effectively can suppress the growth of miscellaneous bacteria in the situation of lower usage amount, and the growth of dialogue Uricularia polytricha almost has no negative effect, is a kind of Chinese medicine miscellaneous bacteria inhibitor of high selectivity.This Chinese medicine miscellaneous bacteria inhibitor directly adds in white Uricularia polytricha medium the effect can played and suppress miscellaneous bacteria.
(2) China is the country maximum with mulberry cultivation area of breeding silkworms in the world, and mulberry tree plantation nationwide, mulberry tree, except picking leaves is bred silkworms, still has a large amount of ramulus moris to waste as sericulture discarded object every year.The present invention develops ramulus mori and cultivates white Uricularia polytricha technology, using ramulus mori bits as white Uricularia polytricha main medium, not only takes full advantage of accessory substance resource the abundantest in Sericultural production process, also for the cultivation matrix of white Uricularia polytricha provides abundant raw material sources.
(3) the present invention also adds magnesium sulfate in the medium, and magnesium elements can promote mycelial growth, improves in fruiting body yield, and has a certain impact to medium and small grade fruit body size and number; After adding magnesium sulfate, the biological transformation ratio of white Uricularia polytricha significantly improves, and output is also more stable.
Embodiment
Below in conjunction with embodiment, rice cultivation method of the present invention is described further.
Embodiment 1: ramulus mori of the present invention cultivates the method for white Uricularia polytricha, the white Uricularia polytricha bacterial classification selected is white fungus CA3.
A) make medium: culture medium prescription is as follows: ramulus mori consider to be worth doing 50 parts, 10 parts, Schisandra sphenanthera Rehd.et WilS dry powder, 5 parts, garlic dry powder, 5 parts, cassia bark dry powder, 5 parts, wooden small pin for the case dry powder, 10 parts, wheat bran, cotton seed hulls 10 parts, sugar 1 part, land plaster 1 part, superphosphate 0.2 part, 0.1 part, magnesium sulfate.
Manufacturing process is as follows: take the ramulus mori bits of respective amount, Schisandra sphenanthera Rehd.et WilS dry powder, garlic dry powder, cassia bark dry powder, wooden small pin for the case dry powder, wheat bran, cotton seed hulls be mixed into major ingredient, pour into as the sugar of clout, land plaster, superphosphate, magnesium sulfate in major ingredient after being dissolved in a small amount of water again, turn to water content reach 50% while add clear water limit.Adjust the pH value of white Uricularia polytricha medium 5.5.
Described ramulus mori can be fresh mulberry bar, branch, also can be the ramulus mori parched.Wherein, fresh ramulus mori is directly applied, and the ramulus mori parched to be soaked in water pulling out after 3 hours drain away the water just apply, ensure ramulus mori bits water content be 6%.
The preparation method of Schisandra sphenanthera Rehd.et WilS dry powder, garlic dry powder, cassia bark dry powder, wooden small pin for the case dry powder is as follows: get Chinese Magnolivine Fruit, garlic, cassia bark powder, wooden small pin for the case powder, respectively adopt volumetric concentration be 60% ethanol leach 2 times, each 1 hour, obtain extract, more namely gained extract removing ethanol is obtained corresponding dry powder.
B) batching pack sterilizing: the medium batching of step a) gained is loaded in pocket, obtained bacterium rod.To heat under bacterium rod is placed in condition of normal pressure sterilizing, keep 10-l2 hour when the temperature stabilization in bag is at 1O0 DEG C, then cooling naturally.
Medium batching is dispensed into rapidly in polypropylene bacterium bag after mixing, in order to avoid windrow is long for standing time, causes and becomes sour.Every bag approximately loads height 18-20cm.
C) inoculate: beat 3-5 inoculation cave in pocket front, when bacterium rod material temperature drop is to less than 25 DEG C, white Uricularia polytricha original seed is inoculated in inoculation cave.
Seeded process operates on aseptic operating platform, needs in advance to carry out ultraviolet disinfection 0.5-1h to operating desk, before inoculation, also will carry out disinfection to the hand medicinal alcohol of the table top in operating desk, article and operator.
D) cultivation: just the bacterium rod of step c gained moves into outdoor booth, the parallel adjacent stacking of bacterium rod.Inoculate after 9-16 days, in the inoculation cave place enlarging oxygenation of pocket, make mycelia obtain sufficient oxygen.Utilize spraying cooling in conjunction with forced ventilation simultaneously, control canopy temperature at 20-25 DEG C, relative moisture 60-70%.18-25 days after inoculation, former base starts to differentiate microtia bud, enters the young ear phase.
The ceiling of described booth covers black and white film, arranges sunshade net above ceiling, erection spray equipment.
Administration period is noted checking pollution condition, as miscellaneous bacteria appears in cave mouth, kills with formaldehyde injection.
Inoculate after 4 days, cave mouth engenders white projection fine hair shape hypha body, and now temperature should control at 20-23 DEG C, and relative moisture controls at 8O-85%.Along with mycelia physiological ripening, there is light yellow globule in white hair ball, now by cave mouth turning, yellow water should be flowed out outside cave, afterwards temperature is raised to about 25 DEG C, yellow water is shunk.
E) management of growth and development stage: long bacterium bag is moved to agaric growing field or agaric growing room, is built into wall type structure, temperature 20-25 DEG C, relative moisture 60-70%, when young ear length is to diameter about 12cm, sprays water in right amount to bacterium bag, makes its auricle moistening.
The method keeping auricle moistening covers newspaper on bacterium bag, then carry out water spray moisturizing.
F) become the management of ear phase: when entity grows to diameter about 12cm, fruit body comes to the ripening period, now stop water spray, temperature maintains 22-24 DEG C, and entering picking time can gather after 6-8 days.
Below the white Uricularia polytricha result of the test of enforcement 1:
Table 1, the test result of embodiment 1
| Biological transformation ratio (%) | White Uricularia polytricha output (g/ rod) | Bacterial contamination rate (%) | Disease condition | Insect pest situation | Pest-resistant, disease resistance |
| 15 | 33 | 0.5 | Nothing | Nothing | By force |
Heavy/dry culturing raw material weight of wherein biological transformation ratio=dry tremella.
Embodiment 2: ramulus mori of the present invention cultivates the method for white Uricularia polytricha, the white Uricularia polytricha bacterial classification selected is white fungus CA3.
A) make medium: culture medium prescription is as follows: ramulus mori consider to be worth doing 65 parts, 20 parts, Schisandra sphenanthera Rehd.et WilS dry powder, 10 parts, garlic dry powder, 10 parts, cassia bark dry powder, 10 parts, wooden small pin for the case dry powder, 20 parts, wheat bran, cotton seed hulls 20 parts, sugar 3 parts, land plaster 3 parts, superphosphate 0.7 part, 0.3 part, magnesium sulfate.
Manufacturing process is as follows: take the ramulus mori bits of respective amount, Schisandra sphenanthera Rehd.et WilS dry powder, garlic dry powder, cassia bark dry powder, wooden small pin for the case dry powder, wheat bran, cotton seed hulls be mixed into major ingredient, pour into as the sugar of clout, land plaster, superphosphate, magnesium sulfate in major ingredient after being dissolved in a small amount of water again, turn to water content reach 60% while add clear water limit.Adjust the pH value of white Uricularia polytricha medium between 6.5.
Described ramulus mori can be fresh mulberry bar, branch, also can be the ramulus mori parched.Wherein, fresh ramulus mori is directly applied, and the ramulus mori parched to be soaked in water pulling out after 5 hours drain away the water just apply, ensure ramulus mori bits water content be 10%.
The preparation method of Schisandra sphenanthera Rehd.et WilS dry powder, garlic dry powder, cassia bark dry powder, wooden small pin for the case dry powder is as follows: get Chinese Magnolivine Fruit, garlic, cassia bark powder, wooden small pin for the case powder, respectively adopt volumetric concentration be 80% ethanol leach 3 times, each 3 hours, obtain extract, more namely gained extract removing ethanol is obtained corresponding dry powder.
Step b), c), d), e), f) identical with case study on implementation 1.
Below the white Uricularia polytricha result of the test of enforcement 2:
Table 2, the test result of embodiment 2
| Biological transformation ratio (%) | White Uricularia polytricha output (g/ rod) | Bacterial contamination rate (%) | Disease condition | Insect pest situation | Pest-resistant, disease resistance |
| 12 | 28 | 0.5 | Nothing | Nothing | By force |
Heavy/dry culturing raw material weight of wherein biological transformation ratio=dry tremella.
Embodiment 3: ramulus mori of the present invention cultivates the method for white Uricularia polytricha, the white Uricularia polytricha bacterial classification selected is white fungus CA3.
A) make medium: culture medium prescription is as follows: ramulus mori consider to be worth doing 60 parts, 15 parts, Schisandra sphenanthera Rehd.et WilS dry powder, 8 parts, garlic dry powder, 8 parts, cassia bark dry powder, 8 parts, wooden small pin for the case dry powder, 15 parts, wheat bran, cotton seed hulls 15 parts, sugar 2 parts, land plaster 2 parts, superphosphate 0.5 part, 0.2 part, magnesium sulfate.
Manufacturing process is as follows: take the ramulus mori bits of respective amount, Schisandra sphenanthera Rehd.et WilS dry powder, garlic dry powder, cassia bark dry powder, wooden small pin for the case dry powder, wheat bran, cotton seed hulls be mixed into major ingredient, pour into as the sugar of clout, land plaster, superphosphate, magnesium sulfate in major ingredient after being dissolved in a small amount of water again, turn to water content reach 55% while add clear water limit.Adjust the pH value of white Uricularia polytricha medium between 6.
Described ramulus mori can be fresh mulberry bar, branch, also can be the ramulus mori parched.Wherein, fresh ramulus mori is directly applied, and the ramulus mori parched to be soaked in water pulling out after 4 hours drain away the water just apply, ensure ramulus mori bits water content be 8%.
The preparation method of Schisandra sphenanthera Rehd.et WilS dry powder, garlic dry powder, cassia bark dry powder, wooden small pin for the case dry powder is as follows: get Chinese Magnolivine Fruit, garlic, cassia bark powder, wooden small pin for the case powder, respectively adopt volumetric concentration be 75% ethanol leach 2 times, each 2 hours, obtain extract, more namely gained extract removing ethanol is obtained corresponding dry powder.
Step b), c), d), e), f) identical with case study on implementation 1.
Below the white Uricularia polytricha result of the test of enforcement 3:
Table 3, the test result of embodiment 3
| Biological transformation ratio (%) | White Uricularia polytricha output (g/ rod) | Bacterial contamination rate (%) | Disease condition | Insect pest situation | Pest-resistant, disease resistance |
| 18 | 38 | 0.3 | Nothing | Nothing | By force |
Heavy/dry culturing raw material weight of wherein biological transformation ratio=dry tremella.
Comparative example: the ramulus mori of prior art cultivates the method for white Uricularia polytricha, the white Uricularia polytricha bacterial classification selected equally is white fungus CA3.
Comparative example comprises the following steps: medium is prepared: weed tree sawdust 68%, cotton seed hulls 15%, wheat bran 15%, land plaster 1%, calcium carbonate 1%.Material and water ratio 1:1-1:2.Pack sterilizing: adopt low-pressure polyethylene plastics cultivation bag, pocket sterilizing more than 100 DEG C keeps 20-22 hour.Bacterium is sent out in inoculation: 4 inoculation caves are made a call in every bag of front, and inoculum concentration is by the bacterial classification of 13.5 × 24 centimetres of gage plastic bags, and every bag connects 25-30 cultivation bag; Bacteria developing period temperature is to be not less than 22 DEG C and do not surpass 32 DEG C as well, and after 30 days, mycelia can walk purseful.Go out syrinx reason: cultivated through 30 days after inoculation, mycelia physiological ripening can start bait operation, sets up earhole in bag side, relative moisture keeps 80%, until fruit body maturation is gathered.
Below the white Uricularia polytricha result of the test of comparative example:
Table 4, the test result of comparative example
Heavy/dry culturing raw material weight of wherein biological transformation ratio=dry tremella.
Integrated comparative embodiments of the invention 1-3 and comparative example obtain following result:
Table 5, Comprehensive Correlation result
Comprehensive Correlation is visible, and method of the present invention compares conventional art, and bacterial contamination rate significantly reduces, resistant to diseases and insects is stronger, is highly suitable for the universal use in south.
Claims (9)
1. ramulus mori cultivates a method for white Uricularia polytricha, it is characterized in that, comprises the following steps:
A) medium is made: culture medium prescription is as follows: ramulus mori bits 50-65 part, Schisandra sphenanthera Rehd.et WilS dry powder 10-20 part, garlic dry powder 5-10 part, cassia bark dry powder 5-10 part, wooden small pin for the case dry powder 5-10 part, wheat bran 10-20 part, cotton seed hulls 10-20 part, sugared 1-3 part, land plaster 1-3 part, superphosphate 0.2-0.7 part, magnesium sulfate 0.1-0.3 part;
Manufacturing process is as follows: take the ramulus mori bits of respective amount, Schisandra sphenanthera Rehd.et WilS dry powder, garlic dry powder, cassia bark dry powder, wooden small pin for the case dry powder, wheat bran, cotton seed hulls be mixed into major ingredient, pour into as the sugar of clout, land plaster, superphosphate, magnesium sulfate in major ingredient after being dissolved in a small amount of water again, turn to water content reach 50-60% while add clear water limit; Adjust the pH value of white Uricularia polytricha medium between 5.5-6.5;
B) batching pack sterilizing: the medium of step a gained batching is loaded in pocket, obtained bacterium rod; To heat under bacterium rod is placed in condition of normal pressure sterilizing, keep 10-l2 hour when the temperature stabilization in bag is at 1O0 DEG C, then cooling naturally;
C) inoculate: beat 3-5 inoculation cave in pocket front, when bacterium rod material temperature drop is to less than 25 DEG C, white Uricularia polytricha original seed is inoculated in inoculation cave;
D) cultivation: just the bacterium rod of step c gained moves into outdoor booth, the parallel adjacent stacking of bacterium rod; Inoculate after 9-16 days, in the inoculation cave place enlarging oxygenation of pocket, make mycelia obtain sufficient oxygen; Utilize spraying cooling in conjunction with forced ventilation simultaneously, control canopy temperature at 20-25 DEG C, relative moisture 60-70%; 18-25 days after inoculation, former base starts to differentiate microtia bud, enters the young ear phase;
E) management of growth and development stage: long bacterium bag is moved to agaric growing field or agaric growing room, is built into wall type structure, temperature 20-25 DEG C, relative moisture 60-70%, when young ear length is to diameter about 12cm, sprays water in right amount to bacterium bag, makes its auricle moistening;
F) become the management of ear phase: when entity grows to diameter about 12cm, fruit body comes to the ripening period, now stop water spray, temperature maintains 22-24 DEG C, and entering picking time can gather after 6-8 days.
2. ramulus mori according to claim 1 cultivates the method for white Uricularia polytricha, it is characterized in that: in step a, and described ramulus mori can be fresh mulberry bar, branch, also can be the ramulus mori parched; Wherein, fresh ramulus mori is directly applied, and the ramulus mori parched is pulled out after will being soaked in water 3-5 hour to drain away the water and just applied, and ensureing that ramulus mori considers water content to be worth doing is 6-10%.
3. ramulus mori according to claim 1 cultivates the method for white Uricularia polytricha, it is characterized in that: in step a, the preparation method of Schisandra sphenanthera Rehd.et WilS dry powder, garlic dry powder, cassia bark dry powder, wooden small pin for the case dry powder is as follows: get Chinese Magnolivine Fruit, garlic, cassia bark powder, wooden small pin for the case powder, respectively adopt volumetric concentration be 60-80% ethanol leaching 2-3 time, each 1-3 hour, obtain extract, more namely gained extract removing ethanol is obtained corresponding dry powder.
4. ramulus mori according to claim 1 cultivates the method for white Uricularia polytricha, it is characterized in that: in step b, and medium batching is dispensed into rapidly in polypropylene bacterium bag after mixing, in order to avoid windrow is long for standing time, causes and becomes sour; Every bag approximately loads height 18-20cm.
5. the method for white Uricularia polytricha is cultivated according to the arbitrary described ramulus mori of claim 1-4, it is characterized in that: in step c, seeded process operates on aseptic operating platform, need in advance to carry out ultraviolet disinfection 0.5-1h to operating desk, before inoculation, also to carry out disinfection to the hand medicinal alcohol of the table top in operating desk, article and operator.
6. ramulus mori according to claim 5 cultivates the method for white Uricularia polytricha, it is characterized in that: in steps d, and the ceiling of described booth covers black and white film, arranges sunshade net above ceiling, erection spray equipment.
7. cultivate the method for white Uricularia polytricha according to ramulus mori according to claim 6, it is characterized in that: in steps d, administration period is noted checking pollution condition, as miscellaneous bacteria appears in cave mouth, kills with formaldehyde injection.
8. ramulus mori according to claim 7 cultivates the method for white Uricularia polytricha, it is characterized in that: in step e, and the method keeping auricle moistening covers newspaper on bacterium bag, then carry out water spray moisturizing.
9. ramulus mori according to claim 8 cultivates the method for white Uricularia polytricha, it is characterized in that: in steps d, inoculates after 4 days, and cave mouth engenders white projection fine hair shape hypha body, and now temperature should control at 20-23 DEG C, and relative moisture controls at 8O-85%; Along with mycelia physiological ripening, there is light yellow globule in white hair ball, now by cave mouth turning, yellow water should be flowed out outside cave, afterwards temperature is raised to about 25 DEG C, yellow water is shunk.
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