CN104798601A - Cultivation method for lentinula edodes - Google Patents

Cultivation method for lentinula edodes Download PDF

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Publication number
CN104798601A
CN104798601A CN201510242568.9A CN201510242568A CN104798601A CN 104798601 A CN104798601 A CN 104798601A CN 201510242568 A CN201510242568 A CN 201510242568A CN 104798601 A CN104798601 A CN 104798601A
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China
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mushroom
parts
implantation methods
lentinula edodes
matrix
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CN201510242568.9A
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徐丹
范佳晨
赵维达
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Zhangjiagang Hongjia Digital Technology Co Ltd
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Zhangjiagang Hongjia Digital Technology Co Ltd
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    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00Cultivation of mushrooms

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  • Life Sciences & Earth Sciences (AREA)
  • Mycology (AREA)
  • Environmental Sciences (AREA)
  • Pest Control & Pesticides (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Mushroom Cultivation (AREA)

Abstract

The invention relates to the technical field of fungus cultivation, in particular to a cultivation method for lentinula edodes. The cultivation method for the lentinula edodes comprises the following steps: (1) preparing a substrate from 0.01 to 0.03 part of carbendazim, 2 to 8 parts of green tea liquor, 1.5 to 2.5 parts of amino acid and 5 to 8 parts of urea, wherein the water content of the substrate is controlled to be 55 to 65 percent, and the pH value of the substrate is controlled to be 5.6 to 5.8; (2) performing bagging; (3) sterilizing a fungus bag; (4) performing inoculating; (5) performing sterilizing; (6) germinating the lentinula edodes; (7) expediting the growth of the lentinula edodes; (8) enabling the lentinula edodes to grow; (9) performing harvesting. The cultivation method for the lentinula edodes has the benefits that the adopted raw materials are pollution-free and are rich in nutrients; chemical preparations, such as a chemical fertilizer, a pesticide and a sterilizing agent, are not used during a cultivating process, so that damage to a human body caused by the chemical preparations is eliminated; the problem of environmental pollution is solved on the premise of ensuring population health; the nutritional value of the lentinula edodes is further ensured; the lentinula edodes fruiting conversion rate is improved obviously, and the lentinula edodes fruiting amount is further improved.

Description

A kind of implantation methods of mushroom
Technical field
The present invention relates to planting fungi technical field, especially a kind of implantation methods of mushroom.
Background technology
Mushroom has high protein, low fat, polysaccharide, several amino acids and multivitamin mushroom food.1. improve body's immunity: lentinan can improve the phagocytic function of Turnover of Mouse Peritoneal Macrophages, also can promote the lymphocytic generation of T, and improves the lymphocytic killing activity of T.2. delay senility: the water extract of mushroom has scavenging action to hydrogen peroxide, have certain eliminating effect to the hydrogen peroxide in body.3. cancer-resisting: Lenlinus edodes cover contains the ribonucleic acid of duplex structure, after entering human body, can produce the interferon with antitumaous effect.4. hypotensive, reducing blood lipid, norcholesterol: containing purine, choline, tyrosine, oxidase and some nucleic acid substances in mushroom, can play the effect of hypotensive, norcholesterol, reducing blood lipid, again can the disease such as prevention of arterial sclerosis, cirrhosis.5. mushroom also plays therapeutic action to diabetes, pulmonary tuberculosis, catarrhal jaundice, neuritis etc., can be used for indigestion, constipation etc. again.Mushroom contains vitamin C, can play and reduce cholesterol, hypotensive effect.Mushroom juice can replace depressor completely, and is free from side effects.Have the black alcohol of wheat that a kind of general vegetables lack in mushroom, it can be converted into vitamin D, promotes the absorption of calcium in body, and can strengthen the ability of human body resist the disease.
At present, at south China most area anti-season mushroom (high temperature mushroom) because the many employings of effect " water culture " of needs moisturizing and cooling and " covering method " are cultivated.
Water culture be namely by water directly to plant provide its growth required for mineral matter, due to water culture plant time, need the water source that flowing is provided, make water consumption large, and because mushroom rod is immersed in water for a long time, not only easily occur the phenomenon of loose rod, and bacterium rod easily goes mouldy, and affects the quality of mushroom rod and the survival rate of mushroom rod.
Be emitted on ridge-up bed ground after covering method and the de-bag of bacterium rod, be close together between bacterium rod, then bacterium rod covers the sandy loam of 2-3cm, but this method have a lot of drawback.
(1) fruiting is slow, (2) product difference, (3) easy infection: owing to being namely placed on ridge-up bed after the de-bag of bacterium rod, and the now bacterium outside mycoderma of rod also prematurity, resist the scarce capacity of outside miscellaneous bacteria invasion, very easily mildew, rotten cylinder is especially very with plum rain season, now high humidity in air, and temperature on ridge-up bed, humidity are more suitable for the Cultivation condition of mushroom rod, thus most of bacterium rod generates " flower bud mushroom " below.Meanwhile, the mushroom that bacterium rod goes out below does not have shape owing to being squeezed and occupies sandy loam, and can not become rubbish as commodity mushroom, mushroom agriculture has just been toppled over after often plucking at all, defines a large amount of wastes.
Summary of the invention
The object of the invention is: overcome deficiency of the prior art, the implantation methods of the mushroom that a kind of output is high, rich in nutritive value, antioxidant effect are good is provided.
In order to solve the problems of the technologies described above, the technical solution used in the present invention is as follows:
An implantation methods for mushroom, described implantation methods comprises the following steps:
(1) substrate preparation: dehydrated potato powder 50-75 part, glucose 4-8 part, land plaster 0.8-1.5 part, wheat bran 10-20 part, rice bran 5-8 part, hard wood chip 10-20 part, carbendazim 0.01-0.03 part, green tea soup 2-8 part, the water content controlling matrix is 55-65%, and the pH value controlling matrix is 5.6-5.8, amino acid/11 .5-2.5 part, urea 5-8 part;
(2) pack: load in low-pressure polyethylene plastic sack by the matrix prepared, described matrix is 6-8h from stirring the time being accomplished to pack end, and every bag as substrate charging 1-1.5kg, obtains required bacterium bag;
(3) bacterium bag sterilizing: autoclaving is carried out to bacterium bag, described pressure is 0.016MPa, and described sterilization time is 2-2.5h;
(4) inoculate: bacterium bag is proceeded in the bacterial classification canopy of thorough disinfection, adopt Open-Architecture Controller, inoculate complete, first a resistant to elevated temperatures plastics collar is enclosed within plastic bag mouth, turnup sack, and by tampon on neck stopper, then with a fritter high pressure resistant plastic film, tampon is wrapped, tie up solid with rope yarn, immediately sterilizing;
(5) sterilizing: by inoculation complete bacterium bag carry out sterilizing at ambient pressure, be rapidly heated to 100 DEG C of retention times be 10-12h;
(6) send out bacterium: under the complete bacterium bag of inoculation is put into the condition of about 25 DEG C, bacterium sent out by " well " character code buttress, and after 30-45d cultivates, mycelium covers with cultivation bag, when mycelial growth, day and night temperature controls as 3-5 DEG C;
(7) urge mushroom: after mycelia is covered with, move to the long mushroom of booth, wall Peng River, refrigerator ground, the relative moisture of control air is 86%, pH is 5.8-6.2, and daytime gives light stimulus, windows evening and gives cold stimulation, makes day and night temperature control as 8-10 DEG C;
(8) long mushroom: through 6 ~ 8 days urge mushroom, sack finds that white former base is when be soya bean size, lays down outer pocket mouth, to bacterium bag water spray, promotes cap differentiation, maintenance air humidity 86-88%;
(9) gather: pluck in time after mushroom cap launches variable color.
Further, useless ear timber is also contained in described matrix.
Further, described useless ear timber adopts out the ear useless ear timber of 3 years, and the diameter of useless ear timber is 3-5cm.
Further, described low-pressure polyethylene plastic sack before charging first as 100-120 DEG C at keep 12-15h, be then cooled to room temperature.
Further, the gas concentration lwevel in described booth is lower than 1%.
Further, described implantation methods also comprises the extermination of disease and insect pest, adopts yellow plate or black light lamps mosquitos and flies in mushroom planting process, corrupt the paying no attention to of removing in time, and sterilizes with limewash.
Further, also the mineral elements such as magnesium, phosphorus, sulphur, potassium are contained in described matrix.
Further, the content of described magnesium, phosphorus, sulphur, potassium is controlled as 0.1%, 0.5%, 0.3%, 0.8%.
Further, also vitamin B1 is contained in described matrix; The content of described vitamin B1 controls to be 0.01%.
Further, the mass parts of described matrix is composed as follows: dehydrated potato powder 65 parts, glucose 6 parts, land plaster 1.2 parts, 15 parts, wheat bran, 7 parts, rice bran, hard wood chip 15 parts, carbendazim 0.02 part, green tea soup 5 parts, controlling the water content of matrix is 60%, and the pH value controlling matrix is 5.7,2 parts, amino acid, 6 parts, urea.
The Advantageous Effects of technical scheme of the present invention is adopted to be: the breeding method of the mushroom of the present invention program, the raw material adopted is pollution-free, nutritious, the chemicals such as chemical fertilizer, agricultural chemicals, disinfectant are not used in cultivating process, thus solve the injury that chemicals brings human body, the problem of environmental pollution is solved again under the prerequisite ensureing population health, also assures that the nutritive value of mushroom, and fruiting conversion ratio have also been obtained and significantly improves, and then improve fruiting amount.
Embodiment
Below in conjunction with specific embodiment, mushroom implantation methods of the present invention is described further.
Embodiment 1
An implantation methods for mushroom, described implantation methods comprises the following steps:
(1) substrate preparation: dehydrated potato powder 50 parts, glucose 4 parts, land plaster 0.8 part, 10 parts, wheat bran, 5 parts, rice bran, hard wood chip 10 parts, carbendazim 0.01 part, green tea soup 2 parts, the water content controlling matrix is 55-65%, and the pH value controlling matrix is 5.6-5.8, amino acid/11 .5 part, 5 parts, urea;
Also containing mineral elements such as magnesium, phosphorus, sulphur, potassium in described matrix; The content of described magnesium, phosphorus, sulphur, potassium is controlled as 0.1%, 0.5%, 0.3%, 0.8%;
Also containing vitamin B1 in described matrix; The content of described vitamin B1 controls to be 0.01%.
(2) pack: load in low-pressure polyethylene plastic sack by the matrix prepared, described matrix is 6-8h from stirring the time being accomplished to pack end, and every bag as substrate charging 1-1.5kg, obtains required bacterium bag;
Preferably, low-pressure polyethylene plastic sack before charging first as 100-120 DEG C at keep 12-15h, be then cooled to room temperature.
(3) bacterium bag sterilizing: autoclaving is carried out to bacterium bag, described pressure is 0.016MPa, and described sterilization time is 2-2.5h;
(4) inoculate: bacterium bag is proceeded in the bacterial classification canopy of thorough disinfection, adopt Open-Architecture Controller, inoculate complete, first a resistant to elevated temperatures plastics collar is enclosed within plastic bag mouth, turnup sack, and by tampon on neck stopper, then with a fritter high pressure resistant plastic film, tampon is wrapped, tie up solid with rope yarn, immediately sterilizing;
(5) sterilizing: by inoculation complete bacterium bag carry out sterilizing at ambient pressure, be rapidly heated to 100 DEG C of retention times be 10-12h;
(6) send out bacterium: under the complete bacterium bag of inoculation is put into the condition of about 25 DEG C, bacterium sent out by " well " character code buttress, and after 30-45d cultivates, mycelium covers with cultivation bag, when mycelial growth, day and night temperature controls as 3-5 DEG C;
(7) urge mushroom: after mycelia is covered with, move to the long mushroom of booth, wall Peng River, refrigerator ground, the relative moisture of control air is 86%, pH is 5.8-6.2, and daytime gives light stimulus, windows evening and gives cold stimulation, makes day and night temperature control as 8-10 DEG C;
(8) long mushroom: through 6 ~ 8 days urge mushroom, sack finds that white former base is when be soya bean size, lays down outer pocket mouth, to bacterium bag water spray, promotes cap differentiation, maintenance air humidity 86-88%;
(9) gather: pluck in time after mushroom cap launches variable color.
Preferably, the gas concentration lwevel in described booth is lower than 1%.
Embodiment 2
Embodiment 2 is compared with embodiment 1, and also containing useless ear timber in matrix, described useless ear timber adopts out the ear useless ear timber of 3 years, and the diameter of useless ear timber is 3-5cm.Other implantation methods are identical.
Embodiment 3
Embodiment 3 is compared with embodiment 1, and described implantation methods also comprises the extermination of disease and insect pest, adopts yellow plate or black light lamps mosquitos and flies in mushroom planting process, corrupt the paying no attention to of removing in time, and sterilizes with limewash.
Also containing useless ear timber in matrix, described useless ear timber adopts out the ear useless ear timber of 3 years, and the diameter of useless ear timber is 3-5cm.Other implantation methods are identical.
Embodiment 4
Embodiment 4 is compared with embodiment 1, in described matrix: dehydrated potato powder 65 parts, glucose 6 parts, land plaster 1.2 parts, 15 parts, wheat bran, 7 parts, rice bran, hard wood chip 15 parts, carbendazim 0.02 part, green tea soup 5 parts, controlling the water content of matrix is 60%, and the pH value controlling matrix is 5.7,2 parts, amino acid, 6 parts, urea.
Other implantation methods are with embodiment 1.
Embodiment 5
Embodiment 4 is compared with embodiment 1, in described matrix: dehydrated potato powder 75 parts, glucose 8 parts, land plaster 1.5 parts, 20 parts, wheat bran, 8 parts, rice bran, hard wood chip 20 parts, carbendazim 0.03 part, green tea soup 8 parts, the water content controlling matrix is 55-65%, and the pH value controlling matrix is 5.6-5.8,2.5 parts, amino acid, 8 parts, urea;
Other implantation methods are with embodiment 1.
Embodiment 3 is preferred embodiment.
Test:
In the present invention, mushroom strain is bought from Yutai County, Shandong Province practical fungus research institute.Composition in mesostroma of the present invention is commercially available.
Compared with the mushroom adopting the mushroom of the method for embodiments of the invention 3 plantation and conventional method to plant, there is following advantage, the low-priceder table 1 of outward appearance.
Relatively content The inventive method is planted General planting method
Mushroom fruiting body Dan Sheng Dan Sheng
Bacteria cover diameter 11.8-12.3cm 6-8cm
Surface color Dark brown is to dark cinnamon Light brown
Bacterial context White Yellow-white
Spore Smooth, colourless Light yellow
Mycelia White, fine hair shape is dense Sparse
Nutrient component compares in table 2
Relatively content The inventive method is planted General planting method
Moisture 12.28±0.02 9.23±0.01
Crude protein 24.18±0.02 19.78±0.02
Crude fat 2.66±0.01 1.58±0.03
Raw fiber 3.40±0.08 2.20±0.09
Coarse ash 6.58±0.05 5.13±0.04
Total reducing sugar 30.89±0.08 25.48±0.04
Dry matter 51.46±0.02 42.89±0.07
Macromolecule polysaccharide 14.12±0.04 9.58±0.06
The above is only the preferred embodiment of the present invention; be not limited to the present invention; should be understood that; for those skilled in the art; under the prerequisite not departing from the technology of the present invention principle; can also make some improvement and modification, these improve and modification also should be considered as protection scope of the present invention.

Claims (10)

1. an implantation methods for mushroom, is characterized in that described implantation methods comprises the following steps:
(1) substrate preparation: dehydrated potato powder 50-75 part, glucose 4-8 part, land plaster 0.8-1.5 part, wheat bran 10-20 part, rice bran 5-8 part, hard wood chip 10-20 part, carbendazim 0.01-0.03 part, green tea soup 2-8 part, the water content controlling matrix is 55-65%, and the pH value controlling matrix is 5.6-5.8, amino acid/11 .5-2.5 part, urea 5-8 part;
(2) pack: load in low-pressure polyethylene plastic sack by the matrix prepared, described matrix is 6-8h from stirring the time being accomplished to pack end, and every bag as substrate charging 1-1.5kg, obtains required bacterium bag;
(3) bacterium bag sterilizing: autoclaving is carried out to bacterium bag, described pressure is 0.016MPa, and described sterilization time is 2-2.5h;
(4) inoculate: bacterium bag is proceeded in the bacterial classification canopy of thorough disinfection, adopt Open-Architecture Controller, inoculate complete, first a resistant to elevated temperatures plastics collar is enclosed within plastic bag mouth, turnup sack, and by tampon on neck stopper, then with a fritter high pressure resistant plastic film, tampon is wrapped, tie up solid with rope yarn, immediately sterilizing;
(5) sterilizing: by inoculation complete bacterium bag carry out sterilizing at ambient pressure, be rapidly heated to 100 DEG C of retention times be 10-12h;
(6) send out bacterium: under the complete bacterium bag of inoculation is put into the condition of about 25 DEG C, bacterium sent out by " well " character code buttress, and after 30-45d cultivates, mycelium covers with cultivation bag, when mycelial growth, day and night temperature controls as 3-5 DEG C;
(7) urge mushroom: after mycelia is covered with, move to the long mushroom of booth, wall Peng River, refrigerator ground, the relative moisture of control air is 86%, pH is 5.8-6.2, and daytime gives light stimulus, windows evening and gives cold stimulation, makes day and night temperature control as 8-10 DEG C;
(8) long mushroom: through 6 ~ 8 days urge mushroom, sack finds that white former base is when be soya bean size, lays down outer pocket mouth, to bacterium bag water spray, promotes cap differentiation, maintenance air humidity 86-88%;
(9) gather: pluck in time after mushroom cap launches variable color.
2. the implantation methods of a kind of mushroom according to claim 1, is characterized in that: also containing useless ear timber in described matrix.
3. the implantation methods of a kind of mushroom according to claim 2, is characterized in that: described useless ear timber adopts out the ear useless ear timber of 3 years, and the diameter of useless ear timber is 3-5cm.
4. the implantation methods of a kind of mushroom according to claim 1, is characterized in that: described low-pressure polyethylene plastic sack before charging first as 100-120 DEG C at keep 12-15h, be then cooled to room temperature.
5. the implantation methods of a kind of mushroom according to claim 1, is characterized in that: the gas concentration lwevel in described booth is lower than 1%.
6. the implantation methods of a kind of mushroom according to claim 1, is characterized in that: described implantation methods also comprises the extermination of disease and insect pest, adopts yellow plate or black light lamps mosquitos and flies in mushroom planting process, corrupt the paying no attention to of removing in time, and sterilizes with limewash.
7. the implantation methods of a kind of mushroom according to claim 1, is characterized in that: also containing mineral elements such as magnesium, phosphorus, sulphur, potassium in described matrix.
8. the implantation methods of a kind of mushroom according to claim 7, is characterized in that: the content of described magnesium, phosphorus, sulphur, potassium is controlled as 0.1%, 0.5%, 0.3%, 0.8%.
9. the implantation methods of a kind of mushroom according to claim 1, is characterized in that: also containing vitamin B1 in described matrix; The content of described vitamin B1 controls to be 0.01%.
10. the implantation methods of a kind of mushroom according to claim 1, it is characterized in that, the mass parts of described matrix is composed as follows: dehydrated potato powder 65 parts, glucose 6 parts, land plaster 1.2 parts, 15 parts, wheat bran, 7 parts, rice bran, hard wood chip 15 parts, carbendazim 0.02 part, green tea soup 5 parts, controlling the water content of matrix is 60%, and the pH value controlling matrix is 5.7,2 parts, amino acid, 6 parts, urea.
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Cited By (14)

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CN105474993A (en) * 2015-11-30 2016-04-13 全椒县香妃农业专业合作社 Snake butter planting method
CN105613047A (en) * 2016-02-26 2016-06-01 云南神奕农业科技有限公司 Planting method of Letinus edodes
CN105706734A (en) * 2016-01-29 2016-06-29 柳州市宣荣农业有限公司 Planting method of shiitake mushrooms
CN105850505A (en) * 2016-04-29 2016-08-17 丽江中源绿色食品有限公司 Shiitake planting method
CN106472105A (en) * 2015-08-31 2017-03-08 佛山市高明区鹏鹄农业发展有限公司 A kind of implantation methods of mushroom
CN107173052A (en) * 2017-05-23 2017-09-19 李贤章 A kind of implantation methods of mushroom
CN107950287A (en) * 2017-11-02 2018-04-24 莫玉艳 A kind of implantation methods of mushroom
CN108271619A (en) * 2018-01-16 2018-07-13 郴州市棪玉种植农民专业合作社 A kind of implantation methods of mushroom
CN108605656A (en) * 2018-04-25 2018-10-02 习水县青野种植有限公司 A kind of mushroom implantation methods
CN108617402A (en) * 2018-04-24 2018-10-09 习水县青野种植有限公司 A kind of method for cultivating mushroom
CN108703015A (en) * 2018-08-23 2018-10-26 安徽农耕年华农业发展有限公司 A kind of mushroom culture medium matter and preparation method thereof
CN109122021A (en) * 2018-08-23 2019-01-04 安徽农耕年华农业发展有限公司 A kind of mushroom cultivating method
CN109275492A (en) * 2018-06-14 2019-01-29 陕西蓝米信息技术有限公司 A kind of mushroom implantation methods
CN109673377A (en) * 2017-08-29 2019-04-26 贵州普定印象朵贝农业开发有限公司 A kind of cultural method of mushroom

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Cited By (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106472105A (en) * 2015-08-31 2017-03-08 佛山市高明区鹏鹄农业发展有限公司 A kind of implantation methods of mushroom
CN105474993A (en) * 2015-11-30 2016-04-13 全椒县香妃农业专业合作社 Snake butter planting method
CN105706734A (en) * 2016-01-29 2016-06-29 柳州市宣荣农业有限公司 Planting method of shiitake mushrooms
CN105613047A (en) * 2016-02-26 2016-06-01 云南神奕农业科技有限公司 Planting method of Letinus edodes
CN105850505A (en) * 2016-04-29 2016-08-17 丽江中源绿色食品有限公司 Shiitake planting method
CN107173052A (en) * 2017-05-23 2017-09-19 李贤章 A kind of implantation methods of mushroom
CN109673377A (en) * 2017-08-29 2019-04-26 贵州普定印象朵贝农业开发有限公司 A kind of cultural method of mushroom
CN107950287A (en) * 2017-11-02 2018-04-24 莫玉艳 A kind of implantation methods of mushroom
CN108271619A (en) * 2018-01-16 2018-07-13 郴州市棪玉种植农民专业合作社 A kind of implantation methods of mushroom
CN108617402A (en) * 2018-04-24 2018-10-09 习水县青野种植有限公司 A kind of method for cultivating mushroom
CN108605656A (en) * 2018-04-25 2018-10-02 习水县青野种植有限公司 A kind of mushroom implantation methods
CN109275492A (en) * 2018-06-14 2019-01-29 陕西蓝米信息技术有限公司 A kind of mushroom implantation methods
CN108703015A (en) * 2018-08-23 2018-10-26 安徽农耕年华农业发展有限公司 A kind of mushroom culture medium matter and preparation method thereof
CN109122021A (en) * 2018-08-23 2019-01-04 安徽农耕年华农业发展有限公司 A kind of mushroom cultivating method

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