CN108703015A - A kind of mushroom culture medium matter and preparation method thereof - Google Patents

A kind of mushroom culture medium matter and preparation method thereof Download PDF

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Publication number
CN108703015A
CN108703015A CN201810963949.XA CN201810963949A CN108703015A CN 108703015 A CN108703015 A CN 108703015A CN 201810963949 A CN201810963949 A CN 201810963949A CN 108703015 A CN108703015 A CN 108703015A
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parts
culture medium
mushroom
degrees celsius
medium matter
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王汉青
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Anhui Farming Times Agriculture Development Co Ltd
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Anhui Farming Times Agriculture Development Co Ltd
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00Cultivation of mushrooms
    • A01G18/20Culture media, e.g. compost
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05DINORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
    • C05D1/00Fertilisers containing potassium
    • C05D1/02Manufacture from potassium chloride or sulfate or double or mixed salts thereof
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05FORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F17/00Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02WCLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00Technologies for solid waste management
    • Y02W30/40Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Health & Medical Sciences (AREA)
  • Inorganic Chemistry (AREA)
  • Mycology (AREA)
  • Environmental Sciences (AREA)
  • Pest Control & Pesticides (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Mushroom Cultivation (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The invention discloses a kind of mushroom culture medium matter and preparation method thereof, it is related to matrix field, including the raw material of following parts by weight meter:0.1-0.6 parts of 70-80 parts of waste wood, 20-30 parts of rice husk, 8-18 parts of cotton seed hull, 20-25 parts of wheatfeed, 10-18 parts of shuck, 6-12 parts of coffee grounds, 4-10 parts of soybean residue, 1-3 parts of calcium carbonate, 0.2-0.5 parts of potassium sulfate, 1-3 parts of gypsum, 0.8-1.5 parts of sucrose and carbendazim;Culture substrate of the present invention is primary raw material using waste wood the like waste, not only reduce the production cost of mushroom, it reduces environmental pollution simultaneously, by the coordinated of each raw material in matrix, abundant ingredient is contained in matrix, contributes to the growth of mushroom mycelium, the mushroom moral character cultivated is good, the miscellaneous bacteria in matrix is reduced by heat sterilization in matrix preparation process simultaneously, to prevent polluting mushroom mycelium.

Description

A kind of mushroom culture medium matter and preparation method thereof
Technical field
The present invention relates to matrix fields, and in particular to a kind of mushroom culture medium matter and preparation method thereof.
Background technology
Mushroom is one of second-biggest-in-the-world edible mushroom and China's specialty, and delicious flavour is full of nutrition, is had prodigious Edible and medical value.Segment wood cultivated and two kinds of substituting stuff cultivation is broadly divided into existing mushroom cultivating method.But it needs a large amount of Timber is only suitable in Development of Forest Area.Substituting stuff cultivation input-output ratio is only 1:2.But substituting stuff cultivation is with short production cycle, biology effect Rate is also high, and can utilize various agricultural wastes, can be environmentally protective in Urban-rural Development.
Chinese patent CN104030822B discloses a kind of mushroom strain matrix and makes the cultivation of mushroom L808 cap yellows Method, matrix mainly include the component of following portions by weight:40-80 parts of Styrax tonkinensis sawdust, common weed tree sawdust 0-40 parts, bran 19 parts of skin, 1 part of land plaster.By the formula and specific cultivation technique is combined, produces that cap is of light color, mushroom is big, rounding The more excellent mushroom product of commodity property, greatly improves the economic benefit of cultivating champignon.But the main material in matrix Material selects Styrax tonkinensis sawdust, and base starting material source is few, limits the large-scale production amount of mushroom.
Chinese patent CN104892102B discloses a kind of culture substrate of mushroom and preparation method thereof;The culture substrate Mass parts composition it is as follows:20-30 parts of wheat bran, 10-20 parts of class reed, 5-8 parts of Jujun grasses, 5-10 parts of napier grass, 5-15 parts of sorghum, stone 2-6 parts of cream powder, 12-25 parts of dregs of beans.Protein from lentinus edodes content height obtained is produced by the formula, it is in good taste, it is full of nutrition, still Relative to using sawdust as the culture substrate of matrix, using Jujun grasses and napier grass as culture substrate, mycelia growth is poor, easily mouldy, fragrant Mushroom finished product moral character is bad.
Invention content
For problems of the prior art, the present invention provides a kind of mushroom culture medium matter and preparation method thereof, originally Invention culture substrate is primary raw material using waste wood the like waste, not only reduces the production cost of mushroom, while reducing ring Border is polluted, and by the coordinated of each raw material in matrix, abundant ingredient is contained in matrix, contributes to the life of mushroom mycelium Long, the mushroom moral character cultivated is good, while the miscellaneous bacteria in matrix is reduced, by heat sterilization with antifouling in matrix preparation process Contaminate mushroom mycelium.
In order to achieve the above object, the present invention is achieved through the following technical solutions:
A kind of mushroom culture medium matter includes the raw material of following parts by weight meter:
70-80 parts of waste wood, 20-30 parts of rice husk, 8-18 parts of cotton seed hull, 20-25 parts of wheatfeed, 10-18 parts of shuck, 6-12 parts of coffee grounds, 4-10 parts of soybean residue, 1-3 parts of calcium carbonate, 0.2-0.5 parts of potassium sulfate, 1-3 parts of gypsum, 0.8-1.5 parts of sucrose With 0.1-0.6 parts of carbendazim.
Preferably, include the raw material of following parts by weight meter:72-78 parts of waste wood, 22-26 parts of rice husk, cotton seed hull 12-14 Part, 21-24 parts of wheatfeed, 12-16 parts of shuck, 8-10 parts of coffee grounds, 6-9 parts of soybean residue, 1.5-2.5 parts of calcium carbonate, sulfuric acid 0.3-0.4 parts of 0.3-0.4 parts of potassium, 1.8-2.6 parts of gypsum, 1-1.3 parts of sucrose and carbendazim.
Preferably, include the raw material of following parts by weight meter:76 parts of waste wood, 24 parts of rice husk, 13 parts of cotton seed hull, wheatfeed 23 parts, 14 parts of shuck, 9 parts of coffee grounds, 8 parts of soybean residue, 2 parts of calcium carbonate, 0.35 part of potassium sulfate, 2.2 parts of gypsum, sucrose 1.1 0.35 part of part and carbendazim.
A kind of preparation method of above-mentioned mushroom culture medium matter is also disclosed in the present invention specifically to include the following steps:
(1) waste wood is placed in pulverizer and be crushed, cross 40-80 mesh sieve, be placed in containing 0.5% hydrogen peroxide and After being impregnated 24 hours in 0.5% sodium hydroxide solution, filtering, merging boiling storehouse is heat-treated 50-60 minutes, and it is broken to obtain waste wood Material;
(2) it after being uniformly mixed rice husk and suitable sodium sulfite, is added in boiling storehouse, at 100-150 degrees Celsius, Under 0.2-0.5MPa, sofening treatment is carried out 5-10 minutes;
(3) shuck crush in merging pulverizer, cross 80-100 mesh sieve, obtain shuck powder;
(4) by nut made from waste wood particle made from step (1), step (2) treated rice husk and step (3) After shell powder is uniformly mixed with coffee grounds, soybean residue, calcium carbonate, potassium sulfate, gypsum and sucrose, mixture is obtained, is added thereto It after entering a certain amount of water, is uniformly mixed, carries out fermentation reactor system, 55-60 degrees Celsius of fermentation temperature, fermentation time 4-6 days, hair It is primary every turning in 8 hours during ferment;
(5) by carbendazim water according to 1:It is mixed with fermentation material and surplus stock stirring after 5 mass ratio carries out mixed diluting It closes uniformly, is packed, material bag is made;
(6) material bag made from step (5) be put into autoclave heating sterilization, after taking the dish out of the pot, material bag is put into modeling 30 degrees Celsius are naturally cooled in material frame hereinafter, to obtain the final product.
Preferably, the heat treatment condition in the step (1) is:80-90 degrees Celsius of vapor (steam) temperature, steam pressure 1.5- 2MPa。
Preferably, the dosage of the sodium sulfite in the step (2) is the 0.8-1.6% of rice husk dosage.
Preferably, the dosage of water is 1.6-2 times of mixture weight in the step (4).
Preferably, the material bag material in the step (5) is low-pressure polyethylene film.
Preferably, the heating sterilization in the step (6) the specific steps are:Big fire is warming up to 100 in 3-5 hours and takes the photograph Family name's degree, moderate heat temperature control maintain 8-10 hours to 100 degrees Celsius, then a stewing night, take the dish out of the pot when material temperature is reduced to 70 degrees Celsius.
Preferably, the frame of plastic in the step (6) is used and need to be sterilized through 75% alcohol wipe.
The present invention has following advantageous effect:
(1) culture substrate of the present invention is primary raw material using waste wood the like waste, not only reduces being produced into for mushroom This, reduces environmental pollution simultaneously, by the coordinated of each raw material in matrix, abundant ingredient is contained in matrix, is contributed to The growth of mushroom mycelium, the mushroom moral character cultivated is good, while being reduced in matrix by heat sterilization in matrix preparation process Miscellaneous bacteria, to prevent polluting mushroom mycelium.
(2) present invention use shuck, coffee grounds and soybean residue to be used as raw material, contain in shuck abundant lignin with Cellulose is beneficial to the growth of mushroom mycelium, and abundant nitride is contained in coffee grounds, can be with the growth of mushroom mycelium, while coffee Coffee slag has good adsorptivity, can adsorb the peculiar smell in culture substrate, contribute to environment pure and fresh, containing abundant in soybean residue Protein, amino acid and Flavonoid substances, the content of lentinus edodes-protein can be improved, enrich mushroom nutritional ingredient, improve The edible value of mushroom.
(3) carbendazim is added in the present invention, a small amount of carbendazim is added in matrix, improves the antimildew and antibacterial effect of matrix Fruit inhibits the breeding of miscellaneous bacteria in matrix, reduces the pollution of miscellaneous bacteria.
(4) waste wood and rice husk are subjected to boiling processing respectively first in preparation method of the present invention, improve rice husk and given up The water absorbency and water retentivity of timber is abandoned, while the volatilization of the nutritional ingredient in culture substrate can be slowed down, is lost in, is beneficial to mycelia Survival and growth.
Specific implementation mode
The specific implementation mode of the present invention is further described with reference to embodiment, following embodiment is only used for more Technical scheme of the present invention is clearly demonstrated, and not intended to limit the protection scope of the present invention.
Embodiment 1
A kind of mushroom culture medium matter includes the raw material of following parts by weight meter:
70 parts of waste wood, 20 parts of rice husk, 8 parts of cotton seed hull, 20 parts of wheatfeed, 10 parts of shuck, 6 parts of coffee grounds, bean curd 0.1 part of 4 parts of slag, 1 part of calcium carbonate, 0.2 part of potassium sulfate, 1 part of gypsum, 0.8 part of sucrose and carbendazim.
The preparation method of mushroom culture medium matter in the present embodiment specifically includes the following steps:
(1) waste wood is placed in pulverizer and be crushed, cross 40 mesh sieve, be placed in containing 0.5% hydrogen peroxide and After being impregnated 24 hours in 0.5% sodium hydroxide solution, filtering, merging boiling storehouse is heat-treated 50 minutes, obtains waste wood particle;
(2) it after being uniformly mixed rice husk and suitable sodium sulfite, is added in boiling storehouse, at 100 degrees Celsius, Under 0.2MPa, sofening treatment is carried out 5 minutes;
(3) shuck crush in merging pulverizer, cross 80 mesh sieve, obtain shuck powder;
(4) by nut made from waste wood particle made from step (1), step (2) treated rice husk and step (3) After shell powder is uniformly mixed with coffee grounds, soybean residue, calcium carbonate, potassium sulfate, gypsum and sucrose, mixture is obtained, is added thereto It after entering a certain amount of water, is uniformly mixed, carries out fermentation reactor system, 55 degrees Celsius of fermentation temperature, fermentation time 4 days fermented Cheng Zhong, it is primary every turning in 8 hours;
(5) by carbendazim water according to 1:It is mixed with fermentation material and surplus stock stirring after 5 mass ratio carries out mixed diluting It closes uniformly, is packed, material bag is made;
(6) material bag made from step (5) be put into autoclave heating sterilization, after taking the dish out of the pot, material bag is put into modeling 30 degrees Celsius are naturally cooled in material frame hereinafter, to obtain the final product.
Wherein, the heat treatment condition in step (1) is:80 degrees Celsius of vapor (steam) temperature, steam pressure 1.5MPa.
The dosage of sodium sulfite in step (2) is the 0.8% of rice husk dosage.
The dosage of water is 1.6 times of mixture weight in step (4).
Material bag material in step (5) is low-pressure polyethylene film.
Heating sterilization in step (6) the specific steps are:Big fire is warming up to 100 degrees Celsius in 3 hours, moderate heat temperature control It to 100 degrees Celsius, maintains 8 hours, then a stewing night, takes the dish out of the pot when material temperature is reduced to 70 degrees Celsius.
Frame of plastic in step (6) is used and need to be sterilized through 75% alcohol wipe.
Embodiment 2
A kind of mushroom culture medium matter includes the raw material of following parts by weight meter:
80 parts of waste wood, 30 parts of rice husk, 18 parts of cotton seed hull, 25 parts of wheatfeed, 18 parts of shuck, 12 parts of coffee grounds, beans 0.6 part of 10 parts of rotten slag, 3 parts of calcium carbonate, 0.5 part of potassium sulfate, 3 parts of gypsum, 1.5 parts of sucrose and carbendazim.
The preparation method of mushroom culture medium matter in the present embodiment specifically includes the following steps:
(1) waste wood is placed in pulverizer and be crushed, cross 80 mesh sieve, be placed in containing 0.5% hydrogen peroxide and After being impregnated 24 hours in 0.5% sodium hydroxide solution, filtering, merging boiling storehouse is heat-treated 60 minutes, obtains waste wood particle;
(2) it after being uniformly mixed rice husk and suitable sodium sulfite, is added in boiling storehouse, at 150 degrees Celsius, Under 0.5MPa, sofening treatment is carried out 10 minutes;
(3) shuck crush in merging pulverizer, sieve with 100 mesh sieve, obtain shuck powder;
(4) by nut made from waste wood particle made from step (1), step (2) treated rice husk and step (3) After shell powder is uniformly mixed with coffee grounds, soybean residue, calcium carbonate, potassium sulfate, gypsum and sucrose, mixture is obtained, is added thereto It after entering a certain amount of water, is uniformly mixed, carries out fermentation reactor system, 60 degrees Celsius of fermentation temperature, fermentation time 6 days fermented Cheng Zhong, it is primary every turning in 8 hours;
(5) by carbendazim water according to 1:It is mixed with fermentation material and surplus stock stirring after 5 mass ratio carries out mixed diluting It closes uniformly, is packed, material bag is made;
(6) material bag made from step (5) be put into autoclave heating sterilization, after taking the dish out of the pot, material bag is put into modeling 30 degrees Celsius are naturally cooled in material frame hereinafter, to obtain the final product.
Wherein, the heat treatment condition in step (1) is:90 degrees Celsius of vapor (steam) temperature, steam pressure 2MPa.
The dosage of sodium sulfite in step (2) is the 1.6% of rice husk dosage.
The dosage of water is 2 times of mixture weight in step (4).
Material bag material in step (5) is low-pressure polyethylene film.
Heating sterilization in step (6) the specific steps are:Big fire is warming up to 100 degrees Celsius in 5 hours, moderate heat temperature control It to 100 degrees Celsius, maintains 10 hours, then a stewing night, takes the dish out of the pot when material temperature is reduced to 70 degrees Celsius.
Frame of plastic in step (6) is used and need to be sterilized through 75% alcohol wipe.
Embodiment 3
A kind of mushroom culture medium matter includes the raw material of following parts by weight meter:
72 parts of waste wood, 22 parts of rice husk, 12 parts of cotton seed hull, 21 parts of wheatfeed, 12 parts of shuck, 8 parts of coffee grounds, bean curd 0.3 part of 6 parts of slag, 1.5 parts of calcium carbonate, 0.3 part of potassium sulfate, 1.8 parts of gypsum, 1 part of sucrose and carbendazim.
The preparation method of mushroom culture medium matter in the present embodiment specifically includes the following steps:
(1) waste wood is placed in pulverizer and be crushed, cross 60 mesh sieve, be placed in containing 0.5% hydrogen peroxide and After being impregnated 24 hours in 0.5% sodium hydroxide solution, filtering, merging boiling storehouse is heat-treated 58 minutes, obtains waste wood particle;
(2) it after being uniformly mixed rice husk and suitable sodium sulfite, is added in boiling storehouse, at 130 degrees Celsius, Under 0.4MPa, sofening treatment is carried out 8 minutes;
(3) shuck crush in merging pulverizer, cross 90 mesh sieve, obtain shuck powder;
(4) by nut made from waste wood particle made from step (1), step (2) treated rice husk and step (3) After shell powder is uniformly mixed with coffee grounds, soybean residue, calcium carbonate, potassium sulfate, gypsum and sucrose, mixture is obtained, is added thereto It after entering a certain amount of water, is uniformly mixed, carries out fermentation reactor system, 58 degrees Celsius of fermentation temperature, fermentation time 5 days fermented Cheng Zhong, it is primary every turning in 8 hours;
(5) by carbendazim water according to 1:It is mixed with fermentation material and surplus stock stirring after 5 mass ratio carries out mixed diluting It closes uniformly, is packed, material bag is made;
(6) material bag made from step (5) be put into autoclave heating sterilization, after taking the dish out of the pot, material bag is put into modeling 30 degrees Celsius are naturally cooled in material frame hereinafter, to obtain the final product.
Wherein, the heat treatment condition in step (1) is:86 degrees Celsius of vapor (steam) temperature, steam pressure 1.8MPa.
The dosage of sodium sulfite in step (2) is the 1.2% of rice husk dosage.
The dosage of water is 1.8 times of mixture weight in step (4).
Material bag material in step (5) is low-pressure polyethylene film.
Heating sterilization in step (6) the specific steps are:Big fire is warming up to 100 degrees Celsius in 4.5 hours, middle fire control Temperature maintains 9 hours to 100 degrees Celsius, then a stewing night, takes the dish out of the pot when material temperature is reduced to 70 degrees Celsius.
Frame of plastic in step (6) is used and need to be sterilized through 75% alcohol wipe.
Embodiment 4
A kind of mushroom culture medium matter includes the raw material of following parts by weight meter:
78 parts of waste wood, 26 parts of rice husk, 14 parts of cotton seed hull, 24 parts of wheatfeed, 16 parts of shuck, 10 parts of coffee grounds, beans 0.4 part of 9 parts of rotten slag, 2.5 parts of calcium carbonate, 0.4 part of potassium sulfate, 2.6 parts of gypsum, 1.3 parts of sucrose and carbendazim.
The preparation method of mushroom culture medium matter in the present embodiment specifically includes the following steps:
(1) waste wood is placed in pulverizer and be crushed, cross 70 mesh sieve, be placed in containing 0.5% hydrogen peroxide and After being impregnated 24 hours in 0.5% sodium hydroxide solution, filtering, merging boiling storehouse is heat-treated 54 minutes, obtains waste wood particle;
(2) it after being uniformly mixed rice husk and suitable sodium sulfite, is added in boiling storehouse, at 120 degrees Celsius, Under 0.35MPa, sofening treatment is carried out 8 minutes;
(3) shuck crush in merging pulverizer, cross 90 mesh sieve, obtain shuck powder;
(4) by nut made from waste wood particle made from step (1), step (2) treated rice husk and step (3) After shell powder is uniformly mixed with coffee grounds, soybean residue, calcium carbonate, potassium sulfate, gypsum and sucrose, mixture is obtained, is added thereto It after entering a certain amount of water, is uniformly mixed, carries out fermentation reactor system, 58 degrees Celsius of fermentation temperature, fermentation time 6 days fermented Cheng Zhong, it is primary every turning in 8 hours;
(5) by carbendazim water according to 1:It is mixed with fermentation material and surplus stock stirring after 5 mass ratio carries out mixed diluting It closes uniformly, is packed, material bag is made;
(6) material bag made from step (5) be put into autoclave heating sterilization, after taking the dish out of the pot, material bag is put into modeling 30 degrees Celsius are naturally cooled in material frame hereinafter, to obtain the final product.
Wherein, the heat treatment condition in step (1) is:88 degrees Celsius of vapor (steam) temperature, steam pressure 1.8MPa.
The dosage of sodium sulfite in step (2) is the 1.4% of rice husk dosage.
The dosage of water is 1.8 times of mixture weight in step (4).
Material bag material in step (5) is low-pressure polyethylene film.
Heating sterilization in step (6) the specific steps are:Big fire is warming up to 100 degrees Celsius in 5 hours, moderate heat temperature control It to 100 degrees Celsius, maintains 9 hours, then a stewing night, takes the dish out of the pot when material temperature is reduced to 70 degrees Celsius.
Frame of plastic in step (6) is used and need to be sterilized through 75% alcohol wipe.
Embodiment 5
A kind of mushroom culture medium matter includes the raw material of following parts by weight meter:
76 parts of waste wood, 24 parts of rice husk, 13 parts of cotton seed hull, 23 parts of wheatfeed, 14 parts of shuck, 9 parts of coffee grounds, bean curd 0.35 part of 8 parts of slag, 2 parts of calcium carbonate, 0.35 part of potassium sulfate, 2.2 parts of gypsum, 1.1 parts of sucrose and carbendazim.
The preparation method of mushroom culture medium matter in the present embodiment specifically includes the following steps:
(1) waste wood is placed in pulverizer and be crushed, cross 50 mesh sieve, be placed in containing 0.5% hydrogen peroxide and After being impregnated 24 hours in 0.5% sodium hydroxide solution, filtering, merging boiling storehouse is heat-treated 52 minutes, obtains waste wood particle;
(2) it after being uniformly mixed rice husk and suitable sodium sulfite, is added in boiling storehouse, at 140 degrees Celsius, Under 0.4MPa, sofening treatment is carried out 8 minutes;
(3) shuck crush in merging pulverizer, cross 90 mesh sieve, obtain shuck powder;
(4) by nut made from waste wood particle made from step (1), step (2) treated rice husk and step (3) After shell powder is uniformly mixed with coffee grounds, soybean residue, calcium carbonate, potassium sulfate, gypsum and sucrose, mixture is obtained, is added thereto It after entering a certain amount of water, is uniformly mixed, carries out fermentation reactor system, 58 degrees Celsius of fermentation temperature, fermentation time 5 days fermented Cheng Zhong, it is primary every turning in 8 hours;
(5) by carbendazim water according to 1:It is mixed with fermentation material and surplus stock stirring after 5 mass ratio carries out mixed diluting It closes uniformly, is packed, material bag is made;
(6) material bag made from step (5) be put into autoclave heating sterilization, after taking the dish out of the pot, material bag is put into modeling 30 degrees Celsius are naturally cooled in material frame hereinafter, to obtain the final product.
Wherein, the heat treatment condition in step (1) is:86 degrees Celsius of vapor (steam) temperature, steam pressure 1.8MPa.
The dosage of sodium sulfite in step (2) is the 1.5% of rice husk dosage.
The dosage of water is 1.7 times of mixture weight in step (4).
Material bag material in step (5) is low-pressure polyethylene film.
Heating sterilization in step (6) the specific steps are:Big fire is warming up to 100 degrees Celsius in 4.5 hours, middle fire control Temperature maintains 9 hours to 100 degrees Celsius, then a stewing night, takes the dish out of the pot when material temperature is reduced to 70 degrees Celsius.
Frame of plastic in step (6) is used and need to be sterilized through 75% alcohol wipe.
Using mushroom culture medium matter culture mushroom made from embodiment 1-5, control group is using on the market using mushroom culture Matrix carries out culture mushroom, and mushroom growth situation see the table below 1:
Table 1
In conclusion the present invention has the following advantages:
(1) culture substrate of the present invention is primary raw material using waste wood the like waste, not only reduces being produced into for mushroom This, reduces environmental pollution simultaneously, by the coordinated of each raw material in matrix, abundant ingredient is contained in matrix, is contributed to The growth of mushroom mycelium, the mushroom moral character cultivated is good, while being reduced in matrix by heat sterilization in matrix preparation process Miscellaneous bacteria, to prevent polluting mushroom mycelium.
(2) present invention use shuck, coffee grounds and soybean residue to be used as raw material, contain in shuck abundant lignin with Cellulose is beneficial to the growth of mushroom mycelium, and abundant nitride is contained in coffee grounds, can be with the growth of mushroom mycelium, while coffee Coffee slag has good adsorptivity, can adsorb the peculiar smell in culture substrate, contribute to environment pure and fresh, containing abundant in soybean residue Protein, amino acid and Flavonoid substances, the content of lentinus edodes-protein can be improved, enrich mushroom nutritional ingredient, improve The edible value of mushroom.
(3) carbendazim is added in the present invention, a small amount of carbendazim is added in matrix, improves the antimildew and antibacterial effect of matrix Fruit inhibits the breeding of miscellaneous bacteria in matrix, reduces the pollution of miscellaneous bacteria.
(4) waste wood and rice husk are subjected to boiling processing respectively first in preparation method of the present invention, improve rice husk and given up The water absorbency and water retentivity of timber is abandoned, while the volatilization of the nutritional ingredient in culture substrate can be slowed down, is lost in, is beneficial to mycelia Survival and growth.
The foregoing is only a preferred embodiment of the present invention, is not intended to restrict the invention, although with reference to aforementioned reality Applying example, invention is explained in detail, for those skilled in the art, still can be to aforementioned each implementation Technical solution recorded in example is modified or equivalent replacement of some of the technical features.All essences in the present invention With within principle, any modification, equivalent replacement, improvement and so on should all be included in the protection scope of the present invention god.

Claims (10)

1. a kind of mushroom culture medium matter, is characterized in that, include the raw material of following parts by weight meter:70-80 parts of waste wood, rice husk 20-30 parts, 8-18 parts of cotton seed hull, 20-25 parts of wheatfeed, 10-18 parts of shuck, 6-12 parts of coffee grounds, 4-10 parts of soybean residue, carbon 0.1-0.6 parts of sour calcium 1-3 parts, 0.2-0.5 parts of potassium sulfate, 1-3 parts of gypsum, 0.8-1.5 parts of sucrose and carbendazim.
2. mushroom culture medium matter according to claim 1, which is characterized in that include the raw material of following parts by weight meter:It is discarded 72-78 parts of timber, 22-26 parts of rice husk, 12-14 parts of cotton seed hull, 21-24 parts of wheatfeed, 12-16 parts of shuck, coffee grounds 8-10 Part, 6-9 parts of soybean residue, 1.5-2.5 parts of calcium carbonate, 0.3-0.4 parts of potassium sulfate, 1.8-2.6 parts of gypsum, 1-1.3 parts of sucrose and more 0.3-0.4 parts of bacterium spirit.
3. mushroom culture medium matter according to claim 1, which is characterized in that include the raw material of following parts by weight meter:It is discarded 76 parts of timber, 24 parts of rice husk, 13 parts of cotton seed hull, 23 parts of wheatfeed, 14 parts of shuck, 9 parts of coffee grounds, 8 parts of soybean residue, calcium carbonate 0.35 part of 2 parts, 0.35 part of potassium sulfate, 2.2 parts of gypsum, 1.1 parts of sucrose and carbendazim.
4. a kind of preparation method of mushroom culture medium matter as described in any one of claims 1-3, which is characterized in that including following Step:
(1) waste wood is placed in pulverizer and be crushed, cross 40-80 mesh sieve, be placed in containing 0.5% hydrogen peroxide and After being impregnated 24 hours in 0.5% sodium hydroxide solution, filtering, merging boiling storehouse is heat-treated 50-60 minutes, and it is broken to obtain waste wood Material;
(2) it after being uniformly mixed rice husk and suitable sodium sulfite, is added in boiling storehouse, at 100-150 degrees Celsius, 0.2- Under 0.5MPa, sofening treatment is carried out 5-10 minutes;
(3) shuck crush in merging pulverizer, cross 80-100 mesh sieve, obtain shuck powder;
(4) by shuck powder made from waste wood particle made from step (1), step (2) treated rice husk and step (3) After being uniformly mixed with coffee grounds, soybean residue, calcium carbonate, potassium sulfate, gypsum and sucrose, mixture is obtained, one is added thereto It after quantitative water, is uniformly mixed, carries out fermentation reactor system, 55-60 degrees Celsius of fermentation temperature, fermentation time 4-6 days fermented Cheng Zhong, it is primary every turning in 8 hours;
(5) by carbendazim water according to 1:After 5 mass ratio carries out mixed diluting, it is stirred with fermentation material and surplus stock It is even, it is packed, material bag is made;
(6) material bag made from step (5) be put into autoclave heating sterilization, after taking the dish out of the pot, material bag is put into frame of plastic In naturally cool to 30 degrees Celsius hereinafter, to obtain the final product.
5. the preparation method of mushroom culture medium matter according to claim 4, which is characterized in that the heat in the step (1) Treatment conditions are:80-90 degrees Celsius of vapor (steam) temperature, steam pressure 1.5-2MPa.
6. the preparation method of mushroom culture medium matter according to claim 4, which is characterized in that the Asia in the step (2) The dosage of sodium sulphate is the 0.8-1.6% of rice husk dosage.
7. the preparation method of mushroom culture medium matter according to claim 4, which is characterized in that water in the step (4) Dosage is 1.6-2 times of mixture weight.
8. the preparation method of mushroom culture medium matter according to claim 4, which is characterized in that the material in the step (5) Bag material is low-pressure polyethylene film.
9. the preparation method of mushroom culture medium matter according to claim 4, which is characterized in that adding in the step (6) Warm sterilization the specific steps are:Big fire is warming up to 100 degrees Celsius in 3-5 hours, moderate heat temperature control maintains 8- to 100 degrees Celsius 10 hours, then a stewing night, it takes the dish out of the pot when material temperature is reduced to 70 degrees Celsius.
10. the preparation method of mushroom culture medium matter according to claim 4, which is characterized in that the modeling in the step (6) Material frame is used and need to be sterilized through 75% alcohol wipe.
CN201810963949.XA 2018-08-23 2018-08-23 A kind of mushroom culture medium matter and preparation method thereof Pending CN108703015A (en)

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CN113951046A (en) * 2021-09-03 2022-01-21 广西南亚热带农业科学研究所 Planting method for interplanting macadamia nut and clitocybe maxima

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Application publication date: 20181026