KR101944613B1 - Medium composite of shiitake and cultivation method using thereof - Google Patents

Abstract

The present invention relates to a culture composition of a mushroom which can produce mushroom with enhanced skin care and anti-cancer effect, and a method of cultivating mushroom using the mushroom. The present invention relates to a method of cultivating mushroom, Preparing a medium material to prepare sawdust, rice bran, calcium carbonate, and rice bran separately from sweet potato and acorn residue, which are left in the cloth; Preparing a medium mixture by mixing the materials prepared in the medium material preparation step and feeding the medium mixture to the medium mixture so that the water content of the medium mixture is 25 to 50% by weight based on the total weight of the medium mixture; Placing the water-containing medium mixture in a transparent container and maintaining the mixture for 1 month to 10 months under sealed anaerobic conditions; Sterilizing the anaerobic treatment-completed medium mixture at 45 to 70 DEG C and cooling to room temperature; Inoculating shiitake mushroom seeds into the sterilized medium mixture; Culturing the medium mixture in which the seedling has been inoculated, culturing and culturing the medium; harvesting; .

Description

TECHNICAL FIELD [0001] The present invention relates to a medium composition for shiitake mushroom and a method for cultivating shiitake mushroom using the same,

The present invention relates to a culture composition of mushroom and a method of cultivating mushroom using the same.

In general, Lentinula edodes is an oriental specialty, rich in high quality protein, fat, various vitamins and minerals, and is treated as a nutritious food with a unique flavor. In addition, recently, pharmacological efficacy has been scientifically proven and widely recognized as a health food.

The natural distribution of shiitake mushrooms is on the Korean Peninsula, Japan, China, Taiwan, the Philippines, Cerebes, New Guinea and Nepal. The natural shiitake mushroom grows in the natural forest by attaching the spore of the shiitake mushroom to the tree which fell on the wind or the dried tree.

In the past, we took advantage of this point and used a primitive cultivation method that leaves an ax mark on the wood slice and waits for the seed to grow with it.

After that, the mushroom cultivated by artificially cultivating mushroom fungus and then inoculating it into the logs has developed into a highland logging method, and the productivity of the elevation has been greatly improved.

However, when the method of planting logs is used, the utilization rate is about 10% of the plant weight, resulting in shortage of hardwood resources, and it is difficult to secure a large amount of plant weight when necessary.

In addition, due to the decrease of rural labor force and the aging of growers, the method of cultivating the logs, which requires a lot of labor, is gradually becoming difficult.

Therefore, it has been used for cultivation of shiitake mushroom for a long time in cultivation of mushroom, mushroom, and mushroom.

In general, rice straw, barley straw, compost, waste paper and the like are used as the medium for the method of culturing the fungus, but sawdust of the hardwood is mainly used in the cultivation method of the mushroom, which is also called sawdust cultivation.

The shiitake cultivation method of shiitake mushrooms can utilize the hardwood resources more efficiently than the log cultivation method with a utilization rate of about 30% to 40% and a utilization rate of about 10%, and the cultivation period is very short, and a lot of the cultivation process can be mechanized Not only is it economical, but the yield of mushrooms is 2-3 times higher than the method of growing logs, which is very active now.

However, the sawdust used in the cultivation of shiitake mushrooms only supplies the basic nutrients related to the growth of mushrooms, and thus it does not properly reflect the needs of Soji bars, which prefer healthier foods.

In other words, recently, consumers are preferred to materials that are easy to ingest, easy to store, and able to simultaneously ingest various nutrients from one material, and mushrooms do not sufficiently reflect the demands of such consumers. But consumption has not been explosively increased.

Reference Document: Korean Patent No. 1632393

The present invention has been made in view of the above-mentioned problems, and it is an object of the present invention to provide a method of cultivating mushroom by mixing sweet potato, acorn, To be able to do so.

In order to achieve the above object, the present invention provides a method for producing a mushroom-containing mushroom culture comprising mixing 80 wt% of sawdust, 10 wt% of rice bran, 1 wt% of calcium carbonate, 2.5 wt% of sweet potato residue, 2.5 wt% of acorn residue, A composition can be prepared.

In addition, the mushroom culture composition may further contain 10 wt% of chalk charcoal, 5 wt% of peanut shell, 2.5 wt% of cottonseed, and 2 wt% of oilseed rape in relation to the weight of the sawdust.

In the case of cultivating mushroom using the mushroom medium composition, the sweet potatoes and acorns with the husks removed are ground with water in a mixer, and then squeezed and squeezed. Then, sweet potatoes and acorn residues left over from the cloth and sawdust, Preparing a medium material for preparing rice bran, calcium carbonate and rice bran; Preparing a medium mixture by mixing the materials prepared in the medium material preparation step and feeding the medium mixture to the medium mixture so that the water content of the medium mixture is 25 to 50% by weight based on the total weight of the medium mixture; Placing the water-containing medium mixture in a transparent container and maintaining the mixture for 1 month to 10 months under sealed anaerobic conditions; Sterilizing the anaerobic treatment-completed medium mixture at 45 to 70 DEG C and cooling to room temperature; Inoculating shiitake mushroom seeds into the sterilized medium mixture; Culturing the medium mixture in which the seedling has been inoculated, culturing and culturing the medium; harvesting; .

At this time, the mulberry bark extract is prepared by mixing the mulberry bark extract and water at a ratio of 1:50 to 1: 100, boiling at high temperature for 2 hours to 3 hours, and then cooling.

The mulberry bark extract is fed to the medium material which has been harvested for one cycle or two cycles.

The culture composition of the mushroom according to the embodiment of the present invention and the method of cultivating the mushroom using the mushroom according to the present invention are obtained by cultivating the mushroom by mixing sweet potato, acorn, It is possible to provide a variety of nutrients in addition to the effect of contributing significantly to the health promotion of consumers.

Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings. In the drawings, the same reference numerals are used to designate the same or similar components throughout the drawings. In the following description of the present invention, a detailed description of known functions and configurations incorporated herein will be omitted when it may make the subject matter of the present invention rather unclear. In addition, the preferred embodiments of the present invention will be described below, but it is needless to say that the technical idea of the present invention is not limited thereto and can be variously modified by those skilled in the art.

The culture composition of mushroom according to an embodiment of the present invention can be prepared by mixing sawdust with rice bran, calcium carbonate, sweet potato starch sludge, acorn residue and rice bran.

The composition ratio of each composition may be 80 wt% of sawdust, 10 wt% of rice bran, 1 wt% of calcium carbonate, 2.5 wt% of sweet potato starch residue, 2.5 wt% of acorn residue and 4 wt% of rice bran.

Also, the mushroom culture medium composition may further contain 10 wt% of chalk charcoal, 5 wt% of peanut shell, 2.5 wt% of cottonseed, and 2 wt% of oil cake based on the weight percentage of sawdust.

That is, the mushroom culture composition of the present invention provides nourishment to mushroom with sawdust, rice bran, calcium carbonate, and rice bran as a main ingredient, and additionally supplies sweet potato starch residue and acorn residue to the vitamins E, C Beta-carotene will provide fatigue recovery and anti-cancer effects to shiitake mushrooms.

In addition, it is possible to further enhance the efficacy of the mushroom by providing antioxidative capacity, prevention of adult disease, blood pressure enhancement, and vascular improvement effect, contained in sweet potato, through the medium composition during the growth of the mushroom.

Acorn residue also contains aconic acid, which acts to absorb and release heavy metals and various harmful substances inside the human body. It is said that there is an excellent effect on fatigue recovery as well as hangover.

Acorn scum is also known to be effective in preventing adult diseases such as diabetes and cancer, and it is known that it has a significant effect on obesity.

Thus, the debris containing the excellent ingredients of sweet potato and acorn can be supplied with the sawdust, rice bran, calcium carbonate and rice bran when the nutrients necessary for the growth of the mushroom are mixed with the mushroom medium composition, It can be greatly increased.

In addition, in the embodiment of the present invention, chalk charcoal, peanut shell, cottonseed, and oil cake may be further included to promote the growth of shiitake mushroom.

It is known that charcoal, charcoal, peanut shell, cottonseed, etc. are not in short supply in supplying nutrients needed for plant growth. Especially, it is effective in growing and fruiting of crops and increasing sugar content of fruits and vegetables. do.

Therefore, when the mushroom is included in the mushroom culture medium composition, the mushroom grows, as well as the taste and sugar content of the mushroom, which is higher than that of the existing mushroom.

In this case, in case of oilseed rape, oil is squeezed out from oil crops such as sesame and perilla, and if remaining remnant is put in the mushroom medium without fermenting, the mushroom germination may not proceed properly, It may be preferable to use them in the next mixing.

Hereinafter, the production of the mushroom culture medium composition and the mushroom cultivation method will be described.

1. Preparation of mushroom medium

10 wt% of chalk carbonized charcoal based on 80 wt% of hardwood sawdust, 10 wt% of rice bran, 1 wt% of calcium carbonate, 2.5 wt% of sweet potato residue, 2.5 wt% of acorn residue, 5% by weight of shell, 2.5% by weight of cottonseed, and 2% by weight of oil cake are prepared.

Rice bran refers to a crushed mixture of crumbs, seed roots, hothouse layers and the like, which are produced when rice cakes are prepared and made into rice cakes. The rice bran can be used within 1 to 30% by weight.

Calcium carbonate acts to control the pH of the sawdust medium and maintains the pH in the range of 5.5 to 6.0 to promote the growth of hyphae.

At this time, the sweet potatoes and acorns removed from the shell are ground with water in a blender, then squeezed in a cloth, and then sweet potatoes and acorn residues left in a cloth are used. Sweet potatoes and acorn scraps are dried in the sun and filtered.

Once all of the ingredients are ready, mix the ingredients together to produce a mixture of media.

2. Moisture supply to media materials

In the process of mixing the medium materials, the mulberry bark extract solution prepared by mixing the mulberry bark extract and water is added to the medium, and until the water content is 25 to 50% by weight based on the mixture of the medium materials, .

Mulberry bark bark is the root bark of mulberry, and it is known that it has an excellent effect on the anti-cancer effect and the improvement of diabetes. It is well known that mulberry bark is excellent for kidney disease, chronic pyelitis and cystitis.

The mulberry bark is dried in the shade and finely ground to mix with the shiitake mushroom medium. However, since the mulberry bark is relatively expensive and the amount produced by mulberry is very limited, Can fall.

Therefore, in the present invention, the mulberry tree bark is crushed and put in the mushroom medium without setting the ratio, and the ratio is set so that the mulberry bark bark and water are mixed in the heating vessel in a ratio of 1:50 to 1: 100, followed by boiling at a high temperature for 2 to 3 hours Use air cooled mulberry bark extract.

The heating time can exceed 3 hours, but boiling for a long time does not greatly improve the effect, so it only concerns about 2 to 3 hours.

Such a mulberry bark extract can be supplied to the medium which has been subjected to the first and one or two cycles in which moisture is supplied to the medium. That is, it is possible to repeatedly inject the mulberry root mallow solution at regular intervals in the process of growing the mushroom, but this case can be applied when the mixing ratio of mulberry root mugwort and water is increased to 1: 500 or more.

3. On the badge material  Maintain anaerobic conditions

In the present invention, the anaerobic condition means a condition in which oxygen is not present in a state in which contact with oxygen is blocked.

In addition, anaerobic means "micro-anaerobic" or "convergent" rather than "absolute anaerobic" with no oxygen.

Place the media material in a clear container. Transparent containers can be made of various materials such as transparent plastic, transparent plastic bags, and zipper packs. The important point is that the structure should satisfy the anaerobic conditions that can be sealed.

If a structure satisfying the anaerobic condition is selected, the anaerobic condition is maintained for at least one month. Preferably for a period of 1 to 10 months or less.

One month is the minimum period to induce anaerobic fermentation, and 10 months is the maximum period. It is not impossible to maintain the anaerobic condition for more than 10 months but it is reasonable to consider the maximum period of 10 months considering the minimum production cost.

Thus, when the medium material maintains the anaerobic condition, the fermentation of alcohol, which is anaerobic fermentation, is induced. In the course of maintaining the anaerobic condition, the medium material maintains a temperature of about 50 ° C to 60 ° C.

The principle that the temperature of the medium mixture can be maintained at 50 캜 to 60 캜 is that 10 to 30% by weight of sugar components such as sucrose, glucose and fructose are contained in the stem of the wood, which is a raw material of sawdust, Since sap contains about 0.1% by weight of sugar, microorganisms release heat while using anaerobic fermentation as a nutrient source.

At the same time, microorganisms secrete enzymes such as cellulase, glycosidase, and xylanase, and the strong structure of the plant cells of sawdust is partially degraded by the action of this enzyme. Therefore, the carbon source and the nitrogen source, which can easily be used by other microorganisms, are almost lost in the anaerobic alcohol fermented medium mixture, and the anaerobic microorganism cells, cellulose and lignin complexes which are not easily decomposed and difficult to use by conventional microorganisms are contained in large amounts .

Shiitake mushroom is a white rot fungus that decomposes cellulosic and lignin complexes well and can be used as an energy source.

In this case, the microorganisms involved in the anaerobic fermentation are anaerobic microorganisms existing in a natural state. Examples of these microorganisms include, for example, Saccharomyces cerevisiae, anaerobic fungus Neocalimumas furontalis, Neocalimumas petrisaarum, Caucasians, and Caucusplaefaciens. These microorganisms exist in the natural state and then enter into the loaded sawdust and cause anaerobic fermentation.

4. Of media material  Sterilization and cooling

The anaerobically treated medium mixture is sterilized at a temperature ranging from 45 to 70 ° C. The sterilization time is set within 1 ~ 10 hours. During the sterilization process, the anaerobic fermenting bacteria that have grown during the anaerobic fermentation process are killed.

After the sterilization treatment has been performed as described above, it is cooled at room temperature.

5. On the badge material  Shiitake mushroom inoculation

The seed material is mixed with the sterilized medium material and inoculated.

The ratio of the seed culture to the culture medium mixture at the time of inoculation of the seed culture is not particularly limited, but the volume ratio of the seed culture: sawdust culture medium = 1: 5: 5-9 is set at a volume ratio.

In this case, the important part is that the moisture content of the medium is maintained at 55 ~ 60%, and care should be taken not to exceed 65% moisture.

This is because when excess water and the number of respiration generated while growing the microorganism are added to the bottom of the mixture of the medium, the problem of hindering the growth of the mycelium occurs.

6. After inoculation Of media material Cancer Culture Cultivation

After inoculation of shiitake mushroom seeds, the inoculated medium is incubated for 30 days at 22 ° C and 60% humidity, and browning is carried out through the medium mixture for 30 days after the completion of the incubation for 30 days.

After the browning process is completed, the mixture is placed in a grower, cultured for one week, and then harvested.

It will be apparent to those skilled in the art that various modifications, substitutions and substitutions are possible, without departing from the scope and spirit of the invention as disclosed in the accompanying claims. will be. Therefore, the embodiments disclosed in the present invention and the accompanying drawings are intended to illustrate and not to limit the technical spirit of the present invention, and the scope of the technical idea of the present invention is not limited by these embodiments and the accompanying drawings . The scope of protection of the present invention should be construed according to the following claims, and all technical ideas within the scope of equivalents should be construed as falling within the scope of the present invention.

Claims (5)

delete delete Preparing a medium for preparing sweet potato and acorn residue, sawdust, rice bran, calcium carbonate, and rice bran each of which is made by wringing sweet potatoes and acorns from which a shell has been removed, grinding it with water in a blender, putting it in a cloth and squeezing it;
Preparing a medium mixture by mixing the materials prepared in the medium material preparation step and feeding the medium mixture to the medium mixture so that the water content of the medium mixture is 25 to 50% by weight based on the total weight of the medium mixture;
Placing the water-containing medium mixture in a transparent container and maintaining the mixture for 1 month to 10 months under sealed anaerobic conditions;
Sterilizing the anaerobic treatment-completed medium mixture at 45 to 70 DEG C and cooling to room temperature;
Inoculating shiitake mushroom seeds into the sterilized medium mixture; And
Culturing and culturing the medium mixture in which the inoculation of seeds has been completed with arm culturing and harvesting; ≪ / RTI >
The method of claim 3,
The mulberry bark extract is prepared by mixing mulberry bark extract and water at a ratio of 1:50 to 1: 100, boiling at a high temperature for 2 hours to 3 hours, and then cooling.
The method of claim 3,
The method of cultivating mushroom cultivation according to claim 1, wherein the mulberry bark extract is fed to a medium material which is added at the time of moisture supply of the medium and has been harvested for one or two cycles.