KR102191161B1 - The Mixture for cultivation of Fragrant Mushroom for increased cell growth speed by invigoration of giant mitochondria - Google Patents

Abstract

The present invention relates to a new method of cultivating shiitake mushrooms, and to a mixed composition capable of improving the growth rate of shiitake mushrooms by activating giant mitochondria of shiitake mushrooms to promote cell metabolism and thereby improving the cell division rate of shiitake mushroom cells. will be.
In other words, the “mixed composition for improving the growth rate through activation of giant mitochondria of shiitake mushrooms” according to the present invention can promote the metabolism of shiitake mushrooms by activating cells in the giant mitochondria, thereby improving cell division speed I can make it. Therefore, as a result, it is possible to increase the growth rate of shiitake mushrooms.
The “mixed composition for improving the growth rate through activation of shiitake mushroom giant mitochondria” according to the present invention includes sodium dehydroacetic acid, citronellyl acetate, propylene glycol alginate, and alginic acid. It is characterized in that it is composed of "ammonium (Ammonium Alginate)".

Description

The Mixture for cultivation of Fragrant Mushroom for increased cell growth speed by invigoration of giant mitochondria}

The present invention relates to a new method of cultivation of shiitake mushrooms, which promotes cell metabolism by activating giant mitochondria of shiitake mushrooms, thereby improving the cell division rate of shiitake mushroom cells, thereby improving the growth rate of shiitake mushrooms. It relates to a new composition that can be mixed.

Shiitake (Lentinus edodes) is an edible mushroom belonging to the genus Basidiomyces and Pleurotus oleracea oleifera pine mushrooms. It is a white edema of wood that grows alone or grows on stumps and stumps from spring to autumn. , It is distributed in regions such as New Zealand (Kim et al., 2003). As a component of shiitake mushrooms, it contains the most moisture, 81.8~90%, and contains protein, amino acids, fats, ash, minerals, vitamins, and dietary fiber. Dietary fiber contains fiber components and various polysaccharides in the cell wall of mushrooms (Bartnicki-Garcia, 1968). As physiological characteristics of shiitake mushrooms, there have been reports of anti-cancer effects, antibacterial properties, hepatitis B treatment effects, and antiviral studies. Among the studies, it has been reported that lentinan, a β-1,3 glucan isolated from the fruiting body of shiitake mushrooms, does not directly exert an anticancer effect, but is involved in host-mediated immune responses to enhance immune function (Chihara ,1992).

The natural distribution of shiitake mushrooms is on the Korean Peninsula, Japan, China, Taiwan, the Philippines, Celebes, New Guinea, and Nepal. Wild shiitake mushrooms are grown by attaching spores (spores) of shiitake mushrooms to trees that have fallen or died in the wind in natural forests.

Conventionally, focusing on this point, a primitive cultivation method was used to make an ax mark on a piece of wood and wait for the spore to grow. After that, the productivity of shiitake has been greatly improved by developing a method of growing shiitake logs by artificially culturing shiitake fungi and then inoculating them on logs.

In particular, oak trees have a high density and a hard material, so a method of cultivating oak shiitake mushrooms has been developed using the characteristics of such oak trees. In other words, a method of cultivating shiitake mushrooms using an open field cultivation method, a facility house cultivation method, and a sawdust cultivation method has already been developed.

However, the existing shiitake mushroom cultivation method is merely a method for cultivating shiitake mushrooms, and few studies or attempts have been made to improve the growth rate by stimulating the substrate in the mitochondria of shiitake mushrooms.

Mitochondria (Mitochondria) is one of the organelles involved in cellular respiration. Therefore, cells with active respiration contain more mitochondria and are called energy-producing factories. In 1897, Carl Benda proved the existence of mitochondria in cells, and the shape was ball-shaped and spring-shaped, and it was said to exist in almost all cytoplasm. As a result of observing the shape, the name was called mitochondria. In Greek, the word'Mitos' meaning thread and'Chondrin' meaning grain or particle were synthesized. I named it. Even before Carl Bender proved the existence of mitochondria, it was known through several scientists. In 1886, German biologist Richard Altmann called mitochondria bioblast, which is a part of life. It was the first to grasp the importance. In addition, mitochondria were called by various names such as chondrosomes, chromidia, and chondroitins, but their existence was not accepted by the scientific community. The mitochondria are surrounded by a double membrane, and the inside is made up of a serpentine inner membrane called Cristae. DNA and RNA exist in it, and the size is 0.2~3㎛ and is involved in cellular respiration. The shape is characterized by each species, and the size is also different depending on the type of cell, but it is usually about 0.5㎛ wide and 2㎛ long. Using a phase-contrast microscope, it can be observed in living cells, and it is stained with Janus Green B to easily distinguish it from other parts. It is also detected by staining by Nadi reaction to cytochrome oxidase and tetrazolium salt reduction reaction by succinate dehydrogenase. The number of mitochondria contained in one cell is related to the energy reception of the cell, and in general, cells with active respiration contain more mitochondria. For example, 1,000-3,000 mitochondria per stem cell and 100-200 mitochondria can be found in plant cells. It is known that there are about 1 caliber of mitochondria in the human body, and about 100,000 in female eggs and about 100 in male sperm. When mitochondria are observed with an electron microscope, very complex structures can be seen. It has a dual structure of the outer membrane and the inner membrane, and the inner membrane looks like a folded maze. Inside the inner membrane, DNA (deoxyribonucleic acid) that carries genetic information is bound. This intimal protrusion is called a crista. Crystas are often arranged in a direction perpendicular to the major axis of the mitochondria. Some cells are arranged in parallel or irregularly. Biologists also regard it as an independent bacterium because of its mitochondrial double membrane and its unique DNA. It is said that there is a high possibility that it was active as an independent bacterium in the beginning.

There are many different enzymes present in the mitochondria.

① Enzymes present in the outer membrane: monoamine oxidase, fatty acid thiokinase, kynurenine hydroxylase, cytochrome C reductase.

② Enzymes present between the inner and outer membranes: adenylate kinase, nucleotide diphosphate kinase

③ Enzymes present in the inner membrane: Ketosan dehydrogenase, succinic acid dehydrogenase, α-β-oxybutyric acid dehydrogenase, carnitine-acyltransferase, terminal electron transfer system ATP synthase

④ Enzymes present in stroma: citric acid synthase, isocitrate dehydrogenase, fumarase, malic acid dehydrogenase, aconitase, glutamic acid dehydrogenase, fatty acid β-oxidase, etc.

The most important function of mitochondria is to synthesize ATP, which is an energy source, through food entering the body. A protein called'ATP synthase' exists in the inner membrane of the mitochondria, which plays a role in producing ATP. Through food, hydrogen ions made between the inner and outer membranes of the mitochondria flow into the inner mitochondrial membrane, and phosphoric acid and ADP (a form of combining two phosphates and adenosine) are combined by ATP synthase to form ATP (three phosphates and adenosine). Combined form) is created. It was Paul Boyer, an American chemist, who discovered the ability of mitochondria to synthesize ATP.

Mitochondria also play a role in killing cells that have lost their function. This is called apoptosis, and the DNA absorbs the destroyed cells or fragments the DNA, leading to death. This is to prevent the cells that have already functioned from being transformed into cancer cells or other cells.

Mitochondria are structures that play a central role in respiration, and are involved in the oxidation of pyruvate and acetyl CoA, which are produced by the catabolic action of lipids, and oxidative phosphorylation through the electron transport system. do. In this way, mitochondria play a central role in the oxidation of sugars and fatty acids and the accompanying oxidative phosphorylation.

In addition, nitrogen is collected as an α-amino group of glutamic acid through an amino group transfer mechanism in various amino acids. There is also an enzyme activity in the urea synthesis system that converts this nitrogen into ammonia by the action of glutamic acid dehydrogenase and then converts it into urea. In addition, part of the reaction of extending the chain of fatty acids by transferring acetyl CoA to fatty acids in the mitochondria is carried out. Mitochondrial functions are sensitive to changes in their structure. For example, mitochondria swollen with thyroxine breathe, but oxidative phosphorylation does not occur.

When the body's muscles are stretched, more mitochondria are produced in more muscle cells, resulting in more energy, that is, ATP. Therefore, stamina is increased and endurance is strengthened. However, if you don't exercise constantly, your mitochondria will be destroyed or your volume will be reduced, so your body's energy will also decrease. However, mitochondria induce free radicals in the process of producing ATP. These free radicals destroy proteins or adversely affect DNA. Cells have the function of defending against free radicals, but their role gradually weakens as they age, resulting in various diseases such as cancer cells, diabetes, high blood pressure, and heart disease.

Mitochondria are extranuclear autoproliferative systems and can multiply themselves. It was confirmed that there is a unique DNA in the mitochondria and a unique protein synthesis system. The mitochondrial DNA and protein synthesis system is similar to that of bacteria. This fact suggests that cardiolipin (a kind of 燐脂質) present in the bacterial cytoplasm is ubiquitous in mitochondria, and that mitochondria have a similar origin to bacteria. In addition, mutations in genes specific to mitochondria are known.

The mitochondria in shiitake mushroom cells are as follows. In shiitake mushrooms, there are several round and circular mitochondria extending close to 50 μm. The mitochondria of shiitake mushrooms are wrapped in two layers of inner and outer mitochondrial membranes. The thickness of the film is about 6 nm, and between the films is 6 to 8 nm, and the inner film protrudes toward the inside and forms a fence-shaped crista with a width of 20 to 30 μm. Mitochondria have a citric acid cycle, an electron transport system, and a group of oxidative phosphorylation enzymes that conjugate to both, and become a field for energy production under aerobic conditions. The substrate contains an enzyme that produces acetyl CoA from pyruvic acid or fatty acids, and an enzyme that oxidizes acetyl CoA by the citric acid cycle. The end products of this oxidation process are CO2 and NADH, CO2 is released from the cells, and NADH is the main source of electrons flowing through the respiratory chain. Crystals and inner membranes are buried with various enzymes involved in electron transport and ATPases involved in oxidative phosphorylation conjugating to them. The basic particle is a part of ATPase, which contains a transmembrane proton carrier, and synthesizes ATP when the proton passes through it according to an electrochemical gradient.

Mitochondria are organelles that produce energy and are surrounded by two layers of membranes. The inner space surrounded by the inner membrane is called a mitochondrial matrix, and it contains not only unique DNA, RNA, and ribosomes, but also various enzymes involved in the TCA cycle, which is the cell respiration stage.

The pyruvate dehydrogenase complex contained in the mitochondrial matrix reacts with the pyruvate produced by the corresponding action when oxygen is sufficient. In this process, CO₂ is released and NADPH, a di-carbon compound, acetyl CoA is produced.

Mitochondria synthesize proteins by themselves using the genetic information present in the matrix, and they can also multiply.

Publication Patent Publication No. 10-2014-0046515 (Publication date 2014.04.21) Unexamined Patent Publication No. 10-2018-0122789 (Publication date 2018.11.14.)

Accordingly, the present invention has been devised to solve the above problems, and provides a mixed composition capable of promoting the cell division of shiitake mushrooms by activating giant mitochondria in shiitake mushroom cells, and thereby improving the growth rate of shiitake mushrooms. It has its main purpose.

Other objects of the present invention are not limited to the objects mentioned above, and other objects that are not mentioned will be clearly understood by those skilled in the art from the following description.

Features of the shiitake mushroom cultivation method according to the present invention for achieving the above object include (A) preparing an oak plant with an epidermis, (B) forming a seed hole in the log, and shiitake in the formed seed hole. Steps of inoculating by injecting mushroom spawns, and (C) laying the wood (alley) into which the shiitake mushroom spawn was added under the conditions of 60% to 70% in shade and 40% to 30% in sunny places, and after 15 days a week The step of performing inoculation tree management through sprinkling once at an interval, (D) the step of erecting an alley in the fall season, which is from November to December or February of the following year, with the scattered logs that have grown more than 80% of mycelium; (E) dissolving a special mixed composition in water to generate a liquid for spraying shiitake mushrooms, and (F) repeatedly spraying the generated liquid for spraying shiitake mushrooms on the alleys every 15 days before harvest. There is a loss.

Preferably, the step (A) is a step of harvesting an oak tree with an epidermis having a diameter of 10 to 20 cm in November-February and drying it in the dark, and reducing the civil engineering length of the dried log oak tree to 1 to 1.2 m. It characterized in that it comprises the step of cutting at intervals and transporting to the inoculation site.

Preferably, in the step (C), the log (alley) into which the shiitake mushroom spawns are put in a well-ventilated and drained place, maintains 70 to 80% humidity, and maintains a temperature of 20 to 28°C. It characterized in that it comprises the steps of performing a bone laying through (井) ruler stacking or pillow stacking, and performing a step of flipping the wood (alley) into which the shiitake mushroom spawns are put into place once a month. .

Preferably, in step (D), prior to erecting the alley, lime or benoram hydrating agent is sprayed in advance, and 20 to 30 pieces per pyeong are erected so that the thin part of the log goes down, so that the distance between the log and the log is about 15 cm in diameter. It characterized in that it comprises a step of making.

Preferably, the constituent material for improving the growth rate of shiitake mushrooms in step (E) is characterized in that it is composed of "sodium dehydroacetate, citroneryl acetate, propylene glycol alginate, ammonium alginate".

Preferably, the amount of sprinkling of the mixed composition solution for improving the growth rate of shiitake mushrooms sprayed in the step (F) is characterized in that sprinkling for about 20 seconds per alley.

As described above, the “mixed composition for improving the growth rate through activation of shiitake mushroom giant mitochondria” according to the present invention can improve the cell division rate of shiitake mushrooms by activating the giant mitochondria. As a result, it is possible to increase the growth rate of shiitake mushrooms.

The "mixed composition for improving the growth rate through activation of shiitake mushroom giant mitochondria" according to the present invention is characterized by consisting of "sodium dehydroacetate, citroneryl acetate, propylene glycol alginate, ammonium alginate".

A detailed description of the “mixed composition for improving the growth rate through activation of shiitake mushroom giant mitochondria” according to the present invention is as follows.

The chemical formula of sodium dehydroacetic acid is C8H7O4Na·H2O. It is a white crystalline powder with no or slight odor and has a slightly distinct taste. It is soluble in water, propylene glycol, and glycerin. It is slightly soluble in alcohol, but is not soluble in ether. It is relatively stable to heat or light and does not decompose even when heat is applied at 120℃ for 2 hours.

When it interacts with metals such as iron or copper, it changes color and does not melt in a metal container. It is easily decomposed by hydrogen peroxide or potassium permanganate and loses its effectiveness. Be careful when using it with sodium chloride solution because it reacts with various metals and is colored. At this time, if the generated metal salt is well dispersed in the food, it does not affect the preservation effect, but if the degree of coloring is large, the value as a product may decrease.

Sodium dihydroacetate is more acidic, the better it is as a preservative, but it is easy to dissociate and has a slight effect in neutral. It is not effective against anaerobic lactic acid bacteria and bacteria of the genus Clostridium, but its effect is excellent against mold, yeast, and anaerobic Gram-positive bacteria at approximately the same concentration. Good for carbohydrate foods like dihydroacetic acid

Citronellyl acetate is a flavoring agent. Used for apricot and peach flavors. It is also used in combinations of rose-based fragrances. It should not be used for any purpose other than flavoring. Citronelol is made by heating with acetic anhydride and sodium acetate. It is a colorless and transparent liquid and has a unique scent. The boiling point is 172~173℃/38mm.

Propylene Glycol Alginate is a food additive to increase the adhesiveness and viscosity of food, improve emulsion stability, and improve the physical properties and texture of food. It is resistant to acid and is used as a thickener and stabilizer for acidic foods.

It is white to yellowish white powder, without smell and tasteless. It dissolves in water or a dilute organic acid solution to become a viscous colloidal solution, and is not soluble in alcohol, chloroform, benzene, and other organic solvents such as petroleum ether. However, depending on the degree of esterification, it is soluble in a 60% aqueous ethanol solution. Sodium alginate is weak in acidity and has a limited range of use, but propylene glycol alginate, which is esterified with propylene oxide of the carboxy group of alginic acid, gels like sodium alginate even in the acidic range of pH 3-4, or does not decrease viscosity like carboxymethylcellulose. Viscosity increases when acid, citric acid, or lactic acid are added. In addition, unlike sodium alginate, which is easily dehydrated and precipitated by alcohol, propylene glycol alginate is highly hydrophilic and stable to alcohol. In the case of a polymer electrolyte, the viscosity decreases when sodium chloride is added, but propylene glycol alginate has a characteristic that the viscosity decreases once and then increases again. In particular, it has excellent resistance to most metal salts except for some heavy metals, so it does not gel or precipitate. In particular, in the case of aqueous solutions, compared to other colloidal solutions, it is stable and does not spoil, but the viscosity gradually decreases. In general, it is stable up to 60℃, but when boiled, the viscosity decreases rapidly, and it is weak to alkali and decomposes into alginate and propylene glycol.

The FAO/WHO Expert Committee's daily allowable intake (ADI) is 0.0~70.0mg/kg. The amount of propylene glycol alginate and the formulation containing it should be less than 1% of food. It is resistant to acid and is used as a thickener and stabilizer for acidic foods. Be sure to use it after dissolving it in water and keep it in a sealed container as it is hygroscopic.

Ammonium Alginate is a food additive to increase the adhesiveness and viscosity of food, improve emulsion stability, and improve the physical properties and texture of food. It is used as a thickener, stabilizer, emulsifier, gelling agent, etc. in food.

The chemical formula is (C6H7O6NH4)n. It is white to pale yellow fibrous, granules, granules, or powder, almost without odor and tasteless. When dissolved in water, it becomes a viscous solution and slowly dissolves in sodium carbonate, sodium hydroxide, and sodium phosphate. In particular, it is not soluble in alcohol, 30% or more alcohol solution, chloroform, ether, and pH 3 or less.

There is no established daily intake (ADI). However, it has been reported that diarrhea symptoms appear when alginates such as alginic acid, ammonium alginate, calcium alginate, potassium alginate and sodium alginate are consumed at high concentrations. It is mainly used as a thickener, stabilizer, emulsifier, gelling agent, etc. in food. When storing, keep in a sealed container.

[Table 1] shows the composition ratio of the “mixed composition for improving growth rate through activation of giant mitochondria of shiitake mushrooms” composed of the above-mentioned substances.

Table 1 is a table showing the composition ratio of the mixed composition for realizing the “mixed composition for improving the growth rate through activation of shiitake mushroom giant mitochondria” according to the present invention. The above composition ratio is the result of finding the most suitable golden ratio through repeated experiments.

Second, the nano-sized mixed composition prepared by the above manufacturing method is subjected to a laser irradiation process. Looking at this in more detail as follows.

(end). Prepare a silicon paper. For silicon paper, buy and use a piece of single crystal silicon with a purity of 99.9999999% cut thinly and smooth the surface. The silicone paper should not have defects or contamination, and it should maintain a high degree of flatness. It is recommended to use a disc shape with a thickness of 0.2 mm and a diameter of 20 to 25 cm.

(I). The nano-sized mixed composition prepared by the above-described method on silicon paper is evenly dispersed in a certain amount. However, the coating thickness is configured to be 0.3mm or less.

(All). After coating to a thickness of 0.3 mm or less as described above on silicon paper, the laser is irradiated thereto.

(la). The wavelength of the laser pulse is 1062nm±4nm, the frequency is 5.2~5.6Hz, and the average output light power is 78~82W laser for 3 minutes.

Through the first and second processes described above, the nassau material composed of the mixed composition has the property of activating the giant mitochondria in the shiitake mushroom cells. When the prepared nano-sized mixed composition is applied to shiitake mushrooms, the nanoparticles effectively activate cells in the giant mitochondria, thereby promoting metabolism. Through this, cell division is promoted and the growth rate of shiitake mushrooms is improved.

Example

1. Mixing process of mixed composition

Sodium dehydroacetic acid 30 parts by weight with an average particle diameter of about 120 nm, Citronellyl acetate 20 parts by weight with an average particle diameter of about 120 nm, Propylene Glycol Alginate with an average particle diameter of about 180 nm ) 30 parts by weight and 20 parts by weight of ammonium alginate having an average particle diameter of about 180 nm were evenly mixed.

2. Laser irradiation process

The mixed composition prepared in step 1 was subjected to laser irradiation in the following process.

(end). Prepare a silicon paper. For silicon paper, buy and use a piece of single crystal silicon with a purity of 99.9999999% cut thinly and smooth the surface. The silicon paper must be free from defects or contamination, as well as maintain a high degree of flatness. A disc-shaped one with a thickness of 0.2 mm and a diameter of 20 to 25 cm was used.

(I). The nano-sized mixed composition prepared by the above-described method on silicon paper is evenly dispersed in a certain amount. However, the coating thickness was configured to be 0.3 mm or less.

(All). After coating to a thickness of 0.3 mm or less as described above on silicon paper, a laser was irradiated thereto.

(la). The laser pulse was irradiated with a laser with a wavelength of 1062 nm±4 nm, a frequency of 5.2 to 5.6 Hz, and an average output light power of 78 to 82 W for 3 minutes.

3. Preparation of mixed composition solution

Water and the mixed composition are mixed at a weight ratio of 1,000:1, and then put into a stirrer and stirred at 500RPM for 5 minutes to prepare a mixed composition solution.

4. Mycelial Growth and Density by using “Water Mixture” in Lentinus edodes experiment with shiitake mushroom ( Lentinus edodes ) using the above-described mixed composition solution

(One). Collection of test materials: 8 types (control, undiluted solution, 200, 400, 600, 800, 1000, 1200 times)

(2). Test design: Mycelial Growth and Density by using OTM in Lentinus edodes of shiitake mushrooms ( Lentinus edodes ) through “Water Mixture”

(3). Mushroom basic sawdust medium composition and column repetition

-Volume 70cc/Weight 50g

-80% cotton thread + 20% rice bran (mix thoroughly and adjust 65%)

-3 repetitions x 5 treatments + Control = 18 columns

-High-pressure sterilization at 121℃ for 40 minutes

-Petri-dish hyphae complete 1cm x 1cm removed

-Dark culture in 25℃ incubation room (dark)

-Measurement of mycelial growth rate (length) every 5 days: marked with a name pen

-When the mycelial growth is completed, put the representative ones together and compare them

-Mycelial density investigation: If the hyphae has grown in a compact way, +++, if it is normal, ++, if it is weak, +

-Compare with control.

-Find the best treatment.

(4). Experiment 1. Shiitake mycelial growth rate experiment according to treatment by concentration of water mixture

-Disclosed strain: Sanjo 701(921)

-Water Mixture Concentration: Control, undiluted solution, 200, 400, 600, 800, 1000, 1200 times

-Material and method: Glass column (8 treatments X 3 repetitions X 1 strain = 24 columns)

Oak sawdust: Rice bran = 8:2 When adjusting moisture after mixing, 3 columns for each concentration of the mixed composition solution (Water Mixture)

-Survey item: Mycelial growth rate (check every 5 days)

Table 2 shows the shiitake hyphae according to the treatment of each concentration of the mixed composition solution (Water Mixture).

It is a table of growth rate.

(5). Experiment 2. Altitude mycelial density experiment by concentration of water mixture

-Disclosure strain: Chalam Aram

-OTM concentration: control, undiluted solution, 200, 400, 600, 800, 1000, 1200 times

-Material and method: Glass column (8 treatments X 3 repetitions X 1 strain = 24 columns)

Oak sawdust: rice bran = 8:2 When adjusting moisture after mixing, 3 columns for each concentration of the mixed composition solution (Water Mixture)

-Survey items: hyphae density (check every 5 days)

Table 3 shows the shiitake hyphae according to the treatment of each concentration of the mixed composition solution (Water Mixture).

It is a table of the results measured after passing 5 days after the start of the experiment on the density. Referring to Table 3 above, it can be seen that the hyphae density is dense in the stock solution, 200 times, 400 times, and 600 times.

As described above, in order to describe the mixed composition for improving the growth rate through activation of giant mitochondria of shiitake mushrooms of the present invention, a specific example was described. However, the technical idea of the present invention is not limited by these specific embodiments, and the degree to which a person skilled in the art can sufficiently change or deform from the claims and detailed description of the present invention is the scope of the present invention. It should be understood as.

Claims (3)

Sodium dehydroacetic acid 29~31wt%, Citronellyl acetate 19.5~20.5wt%, Propylene Glycol Alginate 29~31wt%, and Ammonium Alginate 19.5~20.5 A mixed composition for improving the growth rate through activation of giant mitochondria of shiitake mushrooms, characterized in that consisting of wt%.
The method of claim 1,
The sodium dehydroacetic acid has an average particle size of 120 nm, the citronellyl acetate has an average particle size of 120 nm, and the propylene glycol alginate (Propylene Glycol Alginate) has an average particle size The size is 180nm, the ammonium alginate (Ammonium Alginate) is a mixed composition for improving the growth rate through the activation of the giant mitochondria of shiitake mushrooms, characterized in that the average particle size is 180nm.
Preparing a silicon paper having a purity of 99.9999999% by cutting a single crystal silicon thinly and smoothing the surface thereof;
Uniformly dispersing a predetermined amount of the nano-sized mixed composition on the silicon paper; And
It characterized in that it is manufactured with a step of irradiating a laser; after applying the mixed composition to less than 0.3mm on the silicon paper (siliconpaper),
The silicon paper is free from defects or contamination, maintains a high degree of flatness, has a thickness of 0.2 mm, and a diameter of 20 to 25 cm in the shape of a disk,
The irradiated laser has a pulse wavelength of 1062 nm±4 nm, a frequency of 5.2 to 5.6 Hz, and an average output optical power of 78 to 82 W, and the laser is irradiated for 3 minutes,
The nano-sized mixed composition is sodium dehydroacetic acid 29 to 31 wt%, Citronellyl acetate 19.5 to 20.5 wt%, Propylene Glycol Alginate 29 to 31 wt%, and alginic acid A mixed composition for improving the growth rate through activation of giant mitochondria of shiitake mushrooms, characterized in that consisting of 19.5-20.5wt% of ammonium (Ammonium Alginate).