CN108484270A - A kind of antibacterial peptide bio-feritlizer and preparation method thereof - Google Patents
A kind of antibacterial peptide bio-feritlizer and preparation method thereof Download PDFInfo
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- CN108484270A CN108484270A CN201810469683.3A CN201810469683A CN108484270A CN 108484270 A CN108484270 A CN 108484270A CN 201810469683 A CN201810469683 A CN 201810469683A CN 108484270 A CN108484270 A CN 108484270A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D9/00—Other inorganic fertilisers
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/60—Biocides or preservatives, e.g. disinfectants, pesticides or herbicides; Pest repellants or attractants
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/10—Solid or semi-solid fertilisers, e.g. powders
- C05G5/12—Granules or flakes
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- Life Sciences & Earth Sciences (AREA)
- Pest Control & Pesticides (AREA)
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- Fertilizers (AREA)
Abstract
The invention discloses a kind of antibacterial peptide bio-feritlizers and preparation method thereof, belong to agricultural fertilizer technical field.The fertilizer is made of animal wastes, biomass power plant ash and microbial antibacterial peptide.The preparation method of the fertilizer includes：(1) animal wastes, biomass power plant ash and microorganism material and leavening are weighed by the weight proportion to mix, and adjusts the water content of mixture to 55% 65%, adjusted C/N values to 20 30, adjust pH value to 5.5 7.5；(2) fermentation is stacked for the first time；(3) fermentation is stacked second；(4) it takes the fermentation fertilizer that step (3) obtains to carry out screening and removing impurities matter, granulation, shaping, discharging, drying, cooling, dedusting, detection and packaging, obtains antibacterial peptide bio-feritlizer.The antibacterial peptide bio-feritlizer preparation method method of the present invention is simple, and prepared product has more superior antibacterial insecticidal properties, has the required nutrition of a variety of agriculture and forestry plants, is suitble to cultivate field use in agriculture and forestry plant, improves agriculture and forestry plant survival rate.
Description
Technical field
The invention belongs to agricultural fertilizer technical fields more particularly to a kind of antibacterial peptide bio-feritlizer and preparation method thereof.
Background technology
With the development of aquaculture, excreta environmental pollution getting worse, so how to make farm animal excrement
Turn waste into wealth, does the concern that cycle industry receives insider.On the one hand traditional organic fertilizer fermentation period is longer, often
Can be because fermentation time to be insufficient, fermentation is incomplete, and making it, there are a large amount of harmful bacterias.Therefore the traditional organic fertilizer of a large amount of applications
It often causes that there are harmful bacterias in soil, while soil hardening problem and nutrient loss can be caused.On the other hand, due to changing
Fertilizer largely uses in agricultural production, although agricultural output is greatly improved, brings under such as quality of agricultural product
The problems such as drop, soil hardening, pollution of waterhead, these are all since soil long-term lacking organic matter and chemical fertilizer are excessively used and cause
, to solve these problems most basic method is to reduce the usage amount increase soil organism of chemical fertilizer.And this fertilizer can be reduced
The usage amount of chemical fertilizer can also delay the release of the solid element of oneself formation, uniformly be merged with the nutritional ingredient in soil, simultaneously
Bacterium, fungi and the virus in soil can be effectively killed, to improve and improve soil environment quality.
For the main body of this fertilizer, antibacterial peptide has unique bactericidal mechanism and broad-spectrum antibacterial property, and pathogen is not easy to fight
Bacterium peptide generates the characteristics such as drug resistance, becomes the visual field that new-type fertilizer additive enters people.Studies have shown that in antibacterial peptide fertilizer
Addition antibacterial peptide can inhibit germ reproduction, be effectively improved soil fertility.
Invention content
In view of the above-mentioned deficiencies in the prior art, the technical problem to be solved by the present invention is that providing a kind of antibacterial peptide life
Object fertilizer and preparation method thereof improves antibacterial insecticidal properties after adding antibacterial peptide, has the required nutrition of a variety of agriculture and forestry plants,
It is suitble to cultivate field use in agriculture and forestry plant, improves agriculture and forestry plant survival rate, and beneficial bacterium can improve soil self-restraint nutrient
Ability, to gain factor.
The present invention is achieved through the following technical solutions above-mentioned purpose：
A kind of antibacterial peptide bio-feritlizer, includes the raw material of following weight percent：
Animal wastes 45%-65%, biomass power plant ash 35%-55% and microorganism material and leavening 0.01%-
1%.
The animal wastes are one or more arbitrary ratios in pig manure, cow dung, sheep manure, chicken manure, horsehit, rabbit excrement, birds droppings
Mixture.
The leavening be bacillus S103, bacillus 1.2408, microorganism material be photosynthetic bacteria, actinomyces and
The mixture of one or more arbitrary ratios in saccharomycete.
A kind of preparation method of antibacterial peptide bio-feritlizer, comprises the following steps that：
(1) 40%-65% animal wastes, 30%-55% biomass power plant ash and 0.01%-1% are weighed by weight percentage
Microorganism material and leavening mixing, and the water content for adjusting mixture adjusts C/N values to 20-30, adjusts to 55%-65%
PH value is to 5.5-7.5；
(2) (1) mixture that step obtains is stacked into installation for fermenting stack for the first time and be fermented, by turning over when fermentation
It heap and divulges information oxygen is provided into installation for fermenting, whenever mixture temperature is 55 DEG C~58 DEG C, carries out turning processing, stack fermentation
First time tunning is obtained after 20-25 days；
(3) (2) first time tunning that step obtains is banked up thickness and to 35-38 centimetres stack for the second time and fermented,
Second of stacking fermentation is completely decomposed up to first time tunning, obtains fermentation fertilizer；
(4) take (3) fermentation fertilizer that step obtains carry out screening and removing impurities matter, granulation, shaping, discharging, drying, cooling, dedusting,
Detection and packaging, obtain antibacterial peptide bio-feritlizer.
Preferably, step specifically including (1)：
S1, it animal wastes will be accounted for is dried, crushes and mixing, and take biomass power plant ash for use；By animal wastes, biology
Matter Power Plant Ash is uniformly mixed with microorganism material；
S2, leavening is subjected to fermented and cultured；
S3, zymotic fluid prepared by step S2 is uniformly sprayed in mixture prepared by S1, uniformly spraying potency is not less than
4500 active units/ml, it is to be mixed uniformly after, it is 20-30 and pH that the water content that adjusts product, which is 55%-65%, C/N value,
Value is 5.5-7.5, and semi-finished product are made.
The culture medium of fermented and cultured includes tryptone 10g, yeast powder 5g and NaCl 10g, pH value 7.0.
Condition of culture is：24-27 DEG C of cultivation temperature, agitation revolution 130-160r/min, incubation time 18-22h.
Step (1) in, using urea adjust C/N values, using ammonium hydroxide or phosphorus acid for adjusting pH.
The leavening is bacillus S103, bacillus 1.2408.
The microorganism material is the mixture of one or more arbitrary ratios in photosynthetic bacteria, actinomyces and saccharomycete.
Compared with the existing technology, the present invention has the following technical effects：
The antibacterial peptide fertilizer of the present invention prepared by animal wastes, biomass power plant ash and microorganism material and leavening and
At, use microbial fermentation generate microbial antibacterial peptide, using biomass power plant ash as carrier material, cooperation natural fertilizers move
Object excrement is produced a kind of green, safe and efficient novel microbial fermentation fertilizer by advanced fermentation technique, is suitable for
The plantation of a variety of melons and fruits, forest, vegetables etc..For the main body of this fertilizer, antibacterial peptide has unique bactericidal mechanism and antibacterial wide spectrum
Property, and pathogen is not easy to generate the characteristics such as drug resistance to antibacterial peptide, becomes the visual field that new-type fertilizer additive enters people.Research
Show that antibacterial peptide is added in antibacterial peptide fertilizer can inhibit germ reproduction, be effectively improved soil fertility.
The antibacterial peptide fertilizer of the present invention is that basis carrier prepares bio-feritlizer using biomass power plant ash, is effectively utilized
The ash that biomass electric power plant is come out of the stove, under the premise of controlling ash discharge process, the miscellaneous bacteria content in biomass power plant ash is low, is not required to go out
Bacterium is handled, and in resource set.The present invention has effectively widened the raw material range of microbial manure, and reduce antibacterial peptide fertilizer at
This.The mixture of animal wastes is added in antibacterial peptide fertilizer, nutrient is higher contained by animal wastes, and nutrient is balanced, and ingredient is multiple
It is miscellaneous to include protein, fats, organic acid, cellulose, hemicellulose and various inorganic salts etc., under normal circumstances animal wastes
Quality is coarse, loose porous, moisture is few, organic matter decomposition is fast, can effectively improve soil environment, provides required for the growth of seedling bud
Nutrient, while the temperature in seedbed can be improved, promote seedling development.The preparation method of the antibacterial peptide fertilizer of the present invention is adopted
Bio-feritlizer is prepared with biomass power plant ash is basic carrier, is effectively utilized the ash that biomass electric power plant is come out of the stove, is being controlled
Under the premise of ash discharge process, the miscellaneous bacteria content in biomass power plant ash is low, is not required to sterilization treatment, and in resource set.The present invention has
Effect has widened the raw material range of microbial manure, and reduces the cost of antibacterial peptide fertilizer.This method also improves Power Plant Ash
Utility value, and pollution of the biomass power plant ash to environment can be reduced.
Further, the present invention to number be S103,1.2408 high yield antibacterial peptide bacterial strain carry out fermented and cultured, fermented
Liquid simultaneously screens；Then microorganism material is admixed after mixing animal wastes, biomass power plant ash；Finally by it is above-mentioned the former sift out bacterium
Strain S103,1.2408 zymotic fluid be sprayed at the latter among fermentation fertilizer is made, have and improve forest defending and fighting against diseases ability, and is aobvious
Write the speed of growth for promoting plant seedlings.
Further, LB culture mediums are made of peptone, yeast extract and NaCl.It the advantage is that：Peptone is by pancreas
The albumen that enzymatic treatment is crossed, after processing, the molecular weight of albumen of macromolecular becomes smaller, and bacterium is easier to utilize；In addition, also contain purine,
Growth factor is provided.Peptone provides nitrogen source for the growth of bacterium.Secondly, yeast extract yeast extract in culture medium mainly at
It is amino acid to divide, small peptide, nucleic acid, and b family vitamins, predominantly bacterium provide vitamin and trace element, are bacterial growth institutes
It is necessary.The effect of sodium chloride in the medium is to maintain osmotic pressure, and can provide inorganic salts.
Further, selected bacillus comes from Beijing North and receives biological study institute, and strain is excellent pollution-free.It is produced
Raw antibacterial peptide has carried out test of many times in laboratory, has micro efficient action character, can significantly increase LB cultures
The nutritive validity of base.
Further, the potency that preparation is uniformly sprayed in the semi-finished product is not less than the fermentation of 4500 active units/ml
Liquid, the accurate antibacterial ability of potency are ensured that sprinkling mixing is more uniformly distributed.
Description of the drawings
Fig. 1 is the process flow chart of the preparation method of the antibacterial peptide fermentation fertilizer of the present invention.
Specific implementation mode
Referring to Fig. 1, the preparation method of antibacterial peptide fermentation fertilizer of the present invention is as follows：
S1, the always animal wastes than 45%-65% will be accounted for (one in pig manure, cow dung, sheep manure, chicken manure, horsehit, rabbit excrement, birds droppings
Kind or a variety of arbitrary ratios mixture) be dried, crush and mixing；
S2, measurement account for always more for use than the biomass power plant ash of 35%-55%；
S3, the animal wastes that step S1 is handled are uniformly mixed with the obtained biomass power plant ash of step S2；
S4, actinomyces, saccharomycete or other beneficial bacteriums are mixed into the product of step S3；
S5, by number be S103,1.2408 bacillus carry out fermented and cultured, with LB culture mediums (tryptone 10g,
Yeast powder 5g, NaCl 10g, pH value 7.0) it is cultivated；Condition of culture is：24-27 DEG C of cultivation temperature, agitation revolution 130-
160r/min, incubation time 18-22h；Culture obtains zymotic fluid for subsequently preparing use；
S6, zymotic fluid prepared by step S5 is uniformly sprayed in mixture prepared by step S4, it is not low uniformly to spray potency
In 4500 active units/ml, it is to be mixed uniformly after, it is 20-30 that the water content that adjusts product, which is 55%-65%, C/N value, and
PH value is 5.5-7.5, and semi-finished product are made；
S7, semi-finished product obtained in step S6 stack for the first time and are fermented, fermentation time is 20-25 days, when fermentation
It by turning and divulges information oxygen is provided into installation for fermenting, whenever mixture temperature is more than 55 DEG C, carry out turning processing；
S8, the tunning of step S7 is banked up to 35-38 centimetres of thickness and stack for the second time and is fermented, until hair for the first time
Ferment product is completely decomposed, and fermentation fertilizer is made；
S9, the fermentation fertilizer for obtaining step S8 carry out screening and removing impurities matter, granulation, shaping, discharging, drying, cooling, dedusting,
Detection and packaging, obtain antibacterial peptide biofermentation fertilizer.
Embodiment 1
1, the animal wastes (pig manure, chicken manure, cow dung mixture) of 54kg are weighed, are removed in excrement such as stone, wooden slag, leaf
Deng impurity, it is excrement is dry with machine, crush and mixing；
2, the biomass power plant ash for weighing 45kg carries out bacteria removing, and it is for use to be positioned over dry sterile place；
3, animal wastes and biomass power plant ash are uniformly mixed, photosynthetic bacteria, actinomyces material 0.4kg is added, is positioned over
Dry sterile place is for use；
4, by number be S103,1.2408 bacillus carry out fermented and cultured, with LB culture mediums (tryptone 10g, ferment
Female powder 5g, NaCl 10g, pH value 7.0) it is cultivated；Condition of culture is：24 DEG C, agitation revolution 120r/min of cultivation temperature, training
It supports time 19h, culture obtains zymotic fluid for subsequently preparing use；
5, the zymotic fluid of step 4 preparation is uniformly sprayed in the mixture of preparation, is uniformly sprayed potency and is lived not less than 4500
Property unit/ml, it is to be mixed uniformly after, the water content that adjusts product is 55%, C/N values are 23 and pH value is 6.0, is made half
Finished product；
6, semi-finished product obtained in step 5 stack for the first time and be fermented, fermentation time is 20 days, by turning over when fermentation
It heap and divulges information oxygen is provided into installation for fermenting, whenever mixture temperature is more than 55 DEG C, progress turning processing；
7, the tunning of step 6 is banked up into 35 centimetres of thickness and carries out second of stacking fermentation, until fermentation is produced for the first time
Object is completely decomposed, and fermentation fertilizer is made；
8, the fermentation fertilizer for obtaining step 7 carries out screening and removing impurities matter, granulation, shaping, discharging, drying, cooling, dedusting, inspection
It surveys and packs, obtain antibacterial peptide biofermentation fertilizer.
The bio-feritlizer of the present embodiment fermenting and producing in testing on the spot, under same condition of culture, using the culture of fertilizer
More preferably, faster, statistical data averagely improves growing height 2.5-3.0cm for the buds strains such as spinach, Lettuce, gold zone dish development.
Embodiment 2
1, the animal wastes (pig manure, chicken manure, cow dung mixture) of 56kg are weighed, are removed in excrement such as stone, wooden slag, leaf
Deng impurity, it is excrement is dry with machine, crush and mixing；
2, the biomass power plant ash for weighing 43kg carries out bacteria removing, and it is for use to be positioned over dry sterile place；
3, animal wastes and biomass power plant ash are uniformly mixed, photosynthetic bacteria, actinomyces material 0.5kg is added, is positioned over
Dry sterile place is for use；
4, by number be S103,1.2408 bacillus carry out fermented and cultured, with LB culture mediums (tryptone 10g, ferment
Female powder 5g, NaCl 10g, pH value 7.0) it is cultivated；Condition of culture is：25 DEG C, agitation revolution 140r/min of cultivation temperature, training
It supports time 20h, culture obtains zymotic fluid for subsequently preparing use；
5, the zymotic fluid of step 4 preparation is uniformly sprayed in the mixture of preparation, is uniformly sprayed potency and is lived not less than 4500
Property unit/ml, it is to be mixed uniformly after, the water content that adjusts product is 60%, C/N values are 26 and pH value is 6.5, is made half
Finished product；
6, semi-finished product obtained in step 5 stack for the first time and be fermented, fermentation time is 23 days, by turning over when fermentation
It heap and divulges information oxygen is provided into installation for fermenting, whenever mixture temperature is more than 55 DEG C, progress turning processing；
7, the tunning of step 6 is banked up into 37 centimetres of thickness and carries out second of stacking fermentation, until fermentation is produced for the first time
Object is completely decomposed, and fermentation fertilizer is made；
8, the fermentation fertilizer for obtaining step 7 carries out screening and removing impurities matter, granulation, shaping, discharging, drying, cooling, dedusting, inspection
It surveys and packs, obtain antibacterial peptide biofermentation fertilizer.
The bio-feritlizer of the present embodiment fermenting and producing in testing on the spot, under same condition of culture, using the culture of fertilizer
More preferably, faster, statistical data averagely improves growing height 2.5-3.0cm for the buds strain such as tomato, Brassica rapa L, celery development.
Embodiment 3
1, the animal wastes (pig manure, chicken manure, cow dung mixture) of 60kg are weighed, are removed in excrement such as stone, wooden slag, leaf
Deng impurity, it is excrement is dry with machine, crush and mixing；
2, the biomass power plant ash for weighing 39kg carries out bacteria removing, and it is for use to be positioned over dry sterile place；
3, animal wastes and biomass power plant ash are uniformly mixed, photosynthetic bacteria, saccharomycete material 0.4kg is added, is positioned over
Dry sterile place is for use；
4, by number be S103,1.2408 bacillus carry out fermented and cultured, with LB culture mediums (tryptone 10g, ferment
Female powder 5g, NaCl 10g, pH value 7.0) it is cultivated；Condition of culture is：26 DEG C, agitation revolution 160r/min of cultivation temperature, training
It supports time 21h, culture obtains zymotic fluid for subsequently preparing use；
5, the zymotic fluid of step 4 preparation is uniformly sprayed in the mixture of preparation, is uniformly sprayed potency and is lived not less than 4500
Property unit/ml, it is to be mixed uniformly after, the water content that adjusts product is 65%, C/N values are 29 and pH value is 7.0, is made half
Finished product；
6, semi-finished product obtained in step 5 stack for the first time and be fermented, fermentation time is 25 days, by turning over when fermentation
It heap and divulges information oxygen is provided into installation for fermenting, whenever mixture temperature is more than 55 DEG C, progress turning processing；
7, the tunning of step 6 is banked up into 38 centimetres of thickness and carries out second of stacking fermentation, until fermentation is produced for the first time
Object is completely decomposed, and fermentation fertilizer is made；
8, the fermentation fertilizer for obtaining step 7 carries out screening and removing impurities matter, granulation, shaping, discharging, drying, cooling, dedusting, inspection
It surveys and packs, obtain antibacterial peptide biofermentation fertilizer.
The bio-feritlizer of the present embodiment fermenting and producing in testing on the spot, under same condition of culture, using the culture of fertilizer
More preferably, faster, statistical data averagely improves growth girth diameter 4.0-4.5cm for the buds strains such as spinach, tomato, gold zone dish development.
Embodiment 4
1, the animal wastes (pig manure, chicken manure, cow dung mixture) of 45kg are weighed, are removed in excrement such as stone, wooden slag, leaf
Deng impurity, it is excrement is dry with machine, crush and mixing；
2, the biomass power plant ash for weighing 54.5kg carries out bacteria removing, and it is for use to be positioned over dry sterile place；
3, animal wastes and biomass power plant ash are uniformly mixed, photosynthetic bacteria, saccharomycete material 0.5kg is added, is positioned over
Dry sterile place is for use；
4, by number be S103,1.2408 bacillus carry out fermented and cultured, with LB culture mediums (tryptone 10g, ferment
Female powder 5g, NaCl 10g, pH value 7.0) it is cultivated；Condition of culture is：24 DEG C, agitation revolution 130r/min of cultivation temperature, training
It supports time 18h, culture obtains zymotic fluid for subsequently preparing use；
5, the zymotic fluid of step 4 preparation is uniformly sprayed in the mixture of preparation, is uniformly sprayed potency and is lived not less than 4500
Property unit/ml, it is to be mixed uniformly after, the water content that adjusts product is 55%, C/N values are 20 and pH value is 5.5, is made half
Finished product；
6, semi-finished product obtained in step 5 stack for the first time and be fermented, fermentation time is 25 days, by turning over when fermentation
It heap and divulges information oxygen is provided into installation for fermenting, whenever mixture temperature is more than 55 DEG C, progress turning processing；
7, the tunning of step 6 is banked up into 38 centimetres of thickness and carries out second of stacking fermentation, until fermentation is produced for the first time
Object is completely decomposed, and fermentation fertilizer is made；
8, the fermentation fertilizer for obtaining step 7 carries out screening and removing impurities matter, granulation, shaping, discharging, drying, cooling, dedusting, inspection
It surveys and packs, obtain antibacterial peptide biofermentation fertilizer.
Embodiment 5
1, the animal wastes (pig manure, chicken manure, cow dung mixture) of 65kg are weighed, are removed in excrement such as stone, wooden slag, leaf
Deng impurity, it is excrement is dry with machine, crush and mixing；
2, the biomass power plant ash for weighing 34.8kg carries out bacteria removing, and it is for use to be positioned over dry sterile place；
3, animal wastes and biomass power plant ash are uniformly mixed, saccharomycete, actinomyces material 0.5kg is added, is positioned over
Dry sterile place is for use；
4, by number be S103,1.2408 bacillus carry out fermented and cultured, with LB culture mediums (tryptone 10g, ferment
Female powder 5g, NaCl 10g, pH value 7.0) it is cultivated；Condition of culture is：24 DEG C, agitation revolution 130r/min of cultivation temperature, training
It supports time 18h, culture obtains zymotic fluid for subsequently preparing use；
5, the zymotic fluid of step 4 preparation is uniformly sprayed in the mixture of preparation, is uniformly sprayed potency and is lived not less than 4500
Property unit/ml, it is to be mixed uniformly after, the water content that adjusts product is 65%, C/N values are 30 and pH value is 7.5, is made half
Finished product；
6, semi-finished product obtained in step 5 stack for the first time and be fermented, fermentation time is 24 days, by turning over when fermentation
It heap and divulges information oxygen is provided into installation for fermenting, whenever mixture temperature is more than 55 DEG C, progress turning processing；
7, the tunning of step 6 is banked up into 38 centimetres of thickness and carries out second of stacking fermentation, until fermentation is produced for the first time
Object is completely decomposed, and fermentation fertilizer is made；
8, the fermentation fertilizer for obtaining step 7 carries out screening and removing impurities matter, granulation, shaping, discharging, drying, cooling, dedusting, inspection
It surveys and packs, obtain antibacterial peptide biofermentation fertilizer.
Protection scope of the present invention is not limited to the above embodiments, for those of ordinary skill in the art, if
If the various changes and deformations that carried out to the present invention belong within the scope of the claims in the present invention and equivalent technologies, the meaning of the present invention
Including figure also includes these changes and deforms.
Claims (10)
1. a kind of antibacterial peptide bio-feritlizer, which is characterized in that include the raw material of following weight percent：
Animal wastes 45%-65%, biomass power plant ash 35%-55% and microorganism material and leavening 0.01%-1%.
2. wanting the antibacterial peptide bio-feritlizer described in 1 according to right, which is characterized in that the animal wastes are pig manure, cow dung, sheep
The mixture of one or more arbitrary ratios in excrement, chicken manure, horsehit, rabbit excrement, birds droppings.
3. wanting the antibacterial peptide bio-feritlizer described in 1 according to right, which is characterized in that the leavening be bacillus S103,
Bacillus 1.2408, microorganism material are the mixture of one or more arbitrary ratios in photosynthetic bacteria, actinomyces and saccharomycete.
4. a kind of preparation method of antibacterial peptide bio-feritlizer, which is characterized in that comprise the following steps that：
(1) 40%-65% animal wastes, 30%-55% biomass power plant ash and the micro- lifes of 0.01%-1% are weighed by weight percentage
Object material and leavening mixing, and the water content for adjusting mixture adjusts C/N values to 20-30, adjusts pH value to 55%-65%
To 5.5-7.5；
(2) (1) mixture that step obtains is stacked into installation for fermenting stack for the first time and be fermented, when fermentation by turning and
It divulges information and oxygen is provided into installation for fermenting, whenever mixture temperature is 55 DEG C~58 DEG C, fermentation 20- is stacked in progress turning processing
First time tunning is obtained after 25 days；
(3) (2) first time tunning that step obtains is banked up thickness and to 35-38 centimetres stack for the second time and fermented, second
Secondary stacking fermentation is completely decomposed up to first time tunning, obtains fermentation fertilizer；
(4) (3) fermentation fertilizer that step obtains is taken to carry out screening and removing impurities matter, granulation, shaping, discharging, drying, cooling, dedusting, detection
And packaging, obtain antibacterial peptide bio-feritlizer.
5. the preparation method of antibacterial peptide bio-feritlizer according to claim 4, which is characterized in that the specific packet of step (1)
It includes：
S1, it animal wastes will be accounted for is dried, crushes and mixing, and take biomass power plant ash for use；By animal wastes, biomass electricity
Factory's ash and microorganism material are uniformly mixed；
S2, leavening is subjected to fermented and cultured；
S3, zymotic fluid prepared by step S2 is uniformly sprayed in mixture prepared by S1, uniformly spray potency and live not less than 4500
Property unit/ml, it is to be mixed uniformly after, the water content for adjusting product is that 55%-65%, C/N value are 20-30 and pH value is
5.5-7.5 semi-finished product are made.
6. the preparation method of antibacterial peptide bio-feritlizer according to claim 6, which is characterized in that the culture medium of fermented and cultured
Including tryptone 10g, yeast powder 5g and NaCl 10g, pH value 7.0.
7. the preparation method of antibacterial peptide bio-feritlizer according to claim 6, which is characterized in that the culture item of bacillus
Part is：24-27 DEG C of cultivation temperature, agitation revolution 130-160r/min, incubation time 18-22h.
8. the preparation method of antibacterial peptide bio-feritlizer according to claim 5, which is characterized in that step (1) in, using urine
Element adjusts C/N values, using ammonium hydroxide or phosphorus acid for adjusting pH.
9. the preparation method of antibacterial peptide bio-feritlizer according to claim 5, which is characterized in that the leavening is bud
Spore bacillus S103, bacillus 1.2408.
10. the preparation method of antibacterial peptide bio-feritlizer according to claim 5, which is characterized in that the microorganism object
Material is the mixture of one or more arbitrary ratios in photosynthetic bacteria, actinomyces and saccharomycete.
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| CN201810469683.3A Pending CN108484270A (en) | 2018-05-16 | 2018-05-16 | A kind of antibacterial peptide bio-feritlizer and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110384015A (en) * | 2019-06-26 | 2019-10-29 | 山东慧泰华生物技术有限公司 | A kind of systematic treating method of Citrus Huanglongbing pathogen |
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| CN101774856A (en) * | 2010-01-26 | 2010-07-14 | 凯迪阳光生物能源投资有限公司 | Preparation method of microbial fertilizer |
| CN102557829A (en) * | 2012-01-10 | 2012-07-11 | 袁池 | Antibacterial peptide active organic fertilizer and preparation method thereof |
| CN106747733A (en) * | 2016-12-26 | 2017-05-31 | 航天凯天环保科技股份有限公司 | A kind of method that utilization biomass power plant ash and feces of livestock and poultry produce organic fertilizer |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101774856A (en) * | 2010-01-26 | 2010-07-14 | 凯迪阳光生物能源投资有限公司 | Preparation method of microbial fertilizer |
| CN102557829A (en) * | 2012-01-10 | 2012-07-11 | 袁池 | Antibacterial peptide active organic fertilizer and preparation method thereof |
| CN106747733A (en) * | 2016-12-26 | 2017-05-31 | 航天凯天环保科技股份有限公司 | A kind of method that utilization biomass power plant ash and feces of livestock and poultry produce organic fertilizer |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110384015A (en) * | 2019-06-26 | 2019-10-29 | 山东慧泰华生物技术有限公司 | A kind of systematic treating method of Citrus Huanglongbing pathogen |
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Application publication date: 20180904 |