CN110384015A - A kind of systematic treating method of Citrus Huanglongbing pathogen - Google Patents

A kind of systematic treating method of Citrus Huanglongbing pathogen Download PDF

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CN110384015A
CN110384015A CN201910561662.9A CN201910561662A CN110384015A CN 110384015 A CN110384015 A CN 110384015A CN 201910561662 A CN201910561662 A CN 201910561662A CN 110384015 A CN110384015 A CN 110384015A
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parts
bacteria
thief zone
zone type
suspending agent
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王开运
王红艳
于颀
武玉国
王开元
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Shandong Huitaihua Biotechnology Co ltd
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Shandong Huitaihua Biotechnology Co ltd
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
    • A01G17/005—Cultivation methods
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
    • A01N25/02—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing liquids as carriers, diluents or solvents
    • A01N25/04—Dispersions, emulsions, suspoemulsions, suspension concentrates or gels
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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    • A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/36—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids
    • A01N37/38—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids having at least one oxygen or sulfur atom attached to an aromatic ring system
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
    • A01N43/40—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom six-membered rings
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/72—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms
    • A01N43/82—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms five-membered rings with three ring hetero atoms
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/72—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms
    • A01N43/88—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms six-membered rings with three ring hetero atoms
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N51/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds having the sequences of atoms O—N—S, X—O—S, N—N—S, O—N—N or O-halogen, regardless of the number of bonds each atom has and with no atom of these sequences forming part of a heterocyclic ring
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/10—Animals; Substances produced thereby or obtained therefrom
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05B—PHOSPHATIC FERTILISERS
    • C05B17/00—Other phosphatic fertilisers, e.g. soft rock phosphates, bone meal
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C05G3/80—Soil conditioners
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G5/00—Fertilisers characterised by their form
    • C05G5/10—Solid or semi-solid fertilisers, e.g. powders
    • C05G5/12—Granules or flakes

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  • Health & Medical Sciences (AREA)
  • Pest Control & Pesticides (AREA)
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  • Toxicology (AREA)
  • Soil Sciences (AREA)
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Abstract

The invention discloses a kind of systematic treating methods of Citrus Huanglongbing pathogen, in citrus orchard over-all application mandarin tree dedicated fertilizer, while it being directed to the aqueous suspensions symptomatic treatment that disease plant continuously sprays the first bactericidal suspending agent of Thief zone type, sprayed 1 time within 7-10 days, continuous processing 3-4 times kills pathogenetic bacteria；When mandarin tree wood louse occurs, to the aqueous suspensions of the second Thief zone type insecticidal suspending agent of full tree sprinkling, eliminates and pass malicious insect vector；Wherein the first bactericidal suspending agent of Thief zone type includes antibacterial peptide, chlorogenic acid and the gloomy copper of thiophene, and the second Thief zone type insecticidal suspending agent includes clothianidin, Buprofezin and flonicamid.This 3 technical combinations create the systematic treating method of Citrus Huanglongbing pathogen, good application effect, and morbidity mandarin tree is cured.

Description

A kind of systematic treating method of Citrus Huanglongbing pathogen
Technical field
The present invention relates to plant disease technical fields, particularly relate to a kind of systematic treating method of Citrus Huanglongbing pathogen.
Background technique
The necessary inorganic nutrients of plant growth are N, P, K (nitrogen, phosphorus, potassium) three big element.Nitrogen is protein, nucleic acid, phosphatide Main component, and this three is the important component of plasm, nucleus and biomembrane, they are accounted in vital movement There is special role, wherein nitrogen is otherwise known as the element of life.Fruit tree is in great demand to N, P, K, applies fertilizer in Orange Producing Purpose is exactly to meet fruit tree to the needs of three big elements.
In agricultural production the supply main source of nitrogenous fertilizer be people applied into soil humans and animals excrement, urea, The fertilizer such as ammonium nitrate, ammonium sulfate, ammonium hydrogen carbonate, wherein being that chemically synthesized nitrogenous fertilizer synthesizes ammonia in addition to night soil.Usually apply Phosphate fertilizer is mainly calcium superphosphate, double superhosphate, monoammonium phosphate and Diammonium phosphate (DAP).The potash fertilizer of application be mainly potassium chloride, Potassium sulfate, potassium nitrate, potassium phosphate (monopotassium phosphate, potassium dihydrogen phosphate, PA 800K, potassium polyphosphate) and plant ash, wherein removing It is also synthetics outside plant ash.
China begins to use chemical fertilizer (synthesis ammonia, phosphorus compound and potassium compound) after the 1950s, and especially 20 Since the eighties in century in agricultural volume increase and increasing income, a large amount of uses of chemical fertilizer are performed meritorious deeds never to be obliterated.But more than 40 Nian great Liang of process are applied With chemical fertilizer, also gradually expose that soil acidification, salination, impoverishment, hardened, agricultural product quality is relatively low and pollution of area source aggravates etc. Some defects.So being secure an adequate supply according to China's environment and the bearing capacity in arable land, sustainability, consumer demand difference On the basis of amount meets, with biological organic fertilizer and organic fertilizer replacing fertilizer, Efforts To Develop soil improvement improves agricultural product quality, It is still a very difficult task.
As it can be seen that in order to solve, above-mentioned soil acidification, salination, impoverishment, agricultural product quality is relatively low and pollution of area source aggravates Etc. some defects, a variety of improved procedures are proposed in the prior art, as patent CN101709018A discloses a kind of bulky grain fertilizer Material, discloses " according to the fertilizer requirement and uptake characters of Different Crop, by compound fertilizer, Controlled Release Nitrogen Fertilizer, nitrogen, potash fertilizer compound fertilizer, controlled release Nitrogen, K composite fertilizer, nitrogen, phosphorus, K composite fertilizer are made 2~500 grams, 2.5~12 centimetres of particle diameter " technical solution, however, should Bulky grain fertilizer is mainly made of inorganic and organic chemical fertilizer, long-term or be used for multiple times and will cause soil hardening, fertility decline；Together When, propose water-soluble fertilizer in the prior art, for opposite common fertilizer, water-soluble fertilizer have save it is artificial, save water resource, A variety of advantages such as easy-to-use, water-soluble fertilizer on the market includes the multiple types such as trickle irrigation is used, punching is applied, slight irrigation is used, In now While having the above advantages, there is also some drawbacks of common fertilizer for water-soluble fertilizer；In general, a large amount of in water-soluble fertilizer The raw materials for production of element are also only to be mentioned in water-soluble and insoluble matter residual using common npk nutrient raw material Height, there is no the forms and physicochemical properties that inherently change nutrient, this leads to the common npk nutrient in Water soluble fertilizer Utilization rate in soil is still very low, wherein nitrogen nutrient be largely lost or denitrification after become gaseous nitrogen oxide enter it is big Gas, and phosphorus and potassium then become invalid state by soil fixing, while water-soluble fertilizer, due to having the convenience of application, peasant household is applying During often application is excessive or application is excessively frequent, this causes soil acidification, secondary salinization and soil-borne disease again Evil frequently occurs.
Citrus Huanglongbing pathogen (CitrusHuanglongbing, HLB) is citrus quarantine disease, and major transmission path is Carry disease germs seedling, scion of carrying disease germs, sick tree of citrus is propagated, or is formed by the incoming of diaphorina citri of carrying disease germs (Diaphorinacitri) Then first bacterium source infects diffusion prevalence by infecting mediator diaphorina citri progress large area at mandarin tree young sprout pumping hair initial stage. Between orangery, between sick tree and strong tree mainly by the diaphorina citri spread and epidemic that carries disease germs.At a distance mainly by nursery stock and the scion of carrying disease germs And sick tree is propagated.The spread speed of its disease and diffusion prevalence depend primarily on the generation of mediator diaphorina citri population quantity and Its bacterial bearing rate just easily forms pandemic in cause of disease and diaphorina citri and in the case where deposit.The host range of Citrus Huanglongbing pathogen Including mandarin orange, tangerine, orange, lemon and shaddock class.The technology that control yellow twig is spread both at home and abroad at present is to root out diseased plant, removes hair Pathogeny, the method that the non-disease plant of surrounding is protected.But controlling for Citrus Huanglongbing pathogen sick tree can be cured by having not yet to see Treat technical measures.
Check and inspection discovery, because of chronic administration chemical fertilizer, south China farmland and citrus orchard soil pH value are generally smaller, pH value Generally 4 or so, soil aciditiy is excessive；For soil salt EC value generally 0.5% or so, salinity is also excessively high.Soil aciditiy and salt Divide height, be not suitable for the growth of citrus, root system is undeveloped, and nutrient supply is insufficient, and tree body growing way is weak, and disease-resistant performance reduces, and plant is easy Infection morbidity.
The cause of disease of yellow twig is to parasitize the gramnegative bacterium of bast, can bark tissue, leaf, flower, root and It is detected in fruit.Gramnegative bacterium route of transmission is wide, it is very difficult to be killed.Fruit tree once infects this germ, is It avoids other fruit trees infected, controls the propagation and sprawling of yellow twig, people can only excavate or surgery sick tree, lose huge. Therefore, in the daily management of fruit tree, the prevention and control for carrying out Citrus Huanglongbing pathogen become the most important thing.
Currently, the preventions for Citrus Huanglongbing pathogen are mainly to have two aspects: first is that cutting off the defeated of germ from the root Enter, mainly check on nursery stock, reinforces seedling-wood breeding management, exclude sick seedling in time, select disease-free seedling growth and scion.Two From the communication media of cutting germ, the main method using chemistry, physics prevents and treats wood louse.As it can be seen that in the prior art for citrus The communication media of yellow twig is paid close attention to fewer, mainly or on prevention and treatment wood louse, and has no the prevention and control to other communication medias Means.For example microbial manure and its preparation of a kind of prevention and control Citrus Huanglongbing pathogen are disclosed in patent No. CN201510445778.8 Method, provided microbial manure includes following components: Paecilomyces lilacinus, bacillus licheniformis, Trichoderma and organic matter；Institute State in microbial manure, the viable bacteria content of the Paecilomyces lilacinus not less than 0.2 hundred million/gram, the viable bacteria of the lichens bud pole bacterium Content not less than 0.1 hundred million/gram, the viable bacteria content of the Trichoderma not less than 0.05 hundred million/gram, Paecilomyces lilacinus in foregoing invention, Three kinds of lichens bud pole bacterium, Trichoderma strain collective effects, inhibit or destroy line eggs growth course, thus reduce nematode at Worm rate reduces orchard nematode basis occurrence quantity, cuts off the communication media of yellow twig, have the function that prevention and control Citrus Huanglongbing pathogen, though It can so reach said effect, but itself or a kind of biological organic bacterial manure, thallus content is low, and dosage is big, inconvenient to use.And The main media for propagating yellow twig is also not nematode, generally believes that wood louse is only the malicious insect vector of main biography.Such as Patent No. Disclosed in the patent of CN201610000686.3 a kind of microbial composite bacteria and preparation method thereof and its in prevention and treatment citrus Huanglong The application of sick aspect, the microbial composite bacteria, including Ke Liben series bacillus and bacillus subtilis, the Ke Liben The biological deposits number of series bacillus is CGMCCN0.7996, microbial composite bacteria specifically can for microbe composite bacterial liquid or Complex microbial inoculum can inhibit pathogen, but this composite bacteria agent cannot penetrate fabric tissue, have no therapeutic effect.And And this composite bacteria agent also not no function of desinsection, the communication media insect wood louse of yellow twig can not be cut off, prevention and control are also not achieved The effect of Citrus Huanglongbing pathogen.
As it can be seen that, it is frequent still to there is soil acidification, secondary salinization and soil-borne disease in fertilizer used in the prior art The problem of generation, causes the disease resistance of citrus plant to reduce, and is easy infection yellow twig；It and is in the prior art to yellow twig Unilaterally prevented and treated, and yellow twig route of infection has the biography of the seedling that carries disease germs, scion of carrying disease germs, sick tree propagation or diaphorina citri of carrying disease germs Enter and form the approach such as first bacterium source, only prevents and treats yellow twig from an approach, effect is bad.
Summary of the invention
For the shortcoming of the relevant technologies, the purpose of the present invention is to propose to a kind of systematic treating sides of Citrus Huanglongbing pathogen Method, which includes: application mandarin tree dedicated fertilizer, while continuously spraying the first Thief zone type for disease plant The aqueous suspensions symptomatic treatment of bactericidal suspending agent, and when mandarin tree wood louse occurs, to the second Thief zone type desinsection of full tree sprinkling The aqueous suspensions of suspending agent, this 3 technical combinations create the systematic treating method of Citrus Huanglongbing pathogen, and good application effect, fall ill mandarin orange Tangerine is cured.
Based on above-mentioned purpose, a kind of systematic treating method of Citrus Huanglongbing pathogen provided by the invention is applied comprehensively in citrus orchard With mandarin tree dedicated fertilizer, while the aqueous suspensions for continuously spraying the first bactericidal suspending agent of Thief zone type for disease plant are suited the medicine to the illness Treatment, sprays 1 time for 7-10 days, and continuous processing 3-4 times kills pathogenetic bacteria；When mandarin tree wood louse occurs, to full tree sprinkling second The aqueous suspensions of Thief zone type insecticidal suspending agent are eliminated and pass malicious insect vector；
Wherein the first bactericidal suspending agent of Thief zone type includes antibacterial peptide, chlorogenic acid and the gloomy copper of thiophene, and the second Thief zone type kills Worm suspending agent includes clothianidin, Buprofezin and flonicamid.
The present invention is by reducing soil in citrus orchard over-all application mandarin tree dedicated fertilizer, complete replacing fertilizer and organic fertilizer Earth acidity and salinity improve the content of organic matter, improve and optimize soil microenvironment, create the most suitable soil of Ci trus growth Earth ecological condition promotes root growth, keeps Growth status healthy and strong；Continuously spraying for disease plant includes antibacterial peptide, chlorogenic acid With the aqueous suspensions symptomatic treatment of the first bactericidal suspending agent of Thief zone type of the gloomy copper of thiophene, spray 1 time within 7-10 days, continuous processing 3-4 times, By symptomatic treatment, medicament is penetrated into plant tissue, kills pathogenetic bacteria, and tree body is made to get well growth；In citrus trees When lice occurs, sprinkling includes the second Thief zone type insecticidal suspending agent of clothianidin, Buprofezin and flonicamid, eliminates and passes malicious matchmaker Jie insect avoids being passed to pathogen again.The systematic treating method of Citrus Huanglongbing pathogen is created by above-mentioned 3 technical combinations.
In some embodiments of the invention, the mandarin tree dedicated fertilizer includes that function bacterium and mixed mineral particle carry Body；The function bacterium is adsorbed in the mixed mineral particulate vector, the function bacterium include ultra high efficiency nitrogen-fixing bacteria, phosphate solubilizing bacteria and Potassium solubilizing bacteria；
The ultra high efficiency nitrogen-fixing bacteria, are named as SDTB-0035, and classification naming is bacillus amyloliquefaciens (Bacillus Amyloliquefaciens), it is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, preservation address It is Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, the deposit date is on April 26th, 2019, deposit number CGMCC NO.17635；
The phosphate solubilizing bacteria, is named as SDTB-0043, and classification naming is bacillus amyloliquefaciens (Bacillus Amyloliquefaciens), it is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, preservation address It is Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, the deposit date is on April 26th, 2019, deposit number CGMCC NO.17637；
The potassium solubilizing bacteria, is named as SDTB-0052, and classification naming is bacillus amyloliquefaciens (Bacillus Amyloliquefaciens), it is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, preservation address It is Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, the deposit date is on April 26th, 2019, deposit number CGMCC NO.17639；
The mixed mineral particulate vector includes each component of following parts by weight: 20-40 parts of humic acid, 5-15 parts of carbon phosphorus ashes Stone, 5-15 part niter, 3-5 parts of shells, 5-15 parts of magnesium silicates, 5-15 parts of perlites, 5-20 parts of clays and 0.5-2 parts of trapping agents Hydroxypropyl methyl cellulose.
In some embodiments of the invention, the preparation method of the mixed mineral particulate vector includes the following steps:
(a) by 20-40 parts of humic acid, 5-15 parts of carbonatoapatites, 5-15 parts of niters, 3-5 parts of shells, 5-15 parts of silicic acid Magnesium, 5-15 part perlite and 5-20 parts of clays are crushed to diameter 3.5-5mm, form natural mixed mineral powder；
(b) by 0.5-2 parts of hydroxypropyl methyl celluloses be configured to mass concentration be 5% hydroxypropyl methyl cellulose it is water-soluble Liquid；
In natural mixed mineral powder input mixer, 5% hydroxypropyl methyl cellulose aqueous solution is sprayed under stiring, it will The capture of mineral powder body material；
(c) the mineral powder body material after capturing hydroxypropyl methyl cellulose is transported to pelletizer granulation, and by composite ore The composite ore containing various nutrient elements and porous structure is made in 150-200 DEG C of dryness finalization, quenching to room temperature in composition granule Composition granule carrier.
In some embodiments of the invention, the mandarin tree dedicated fertilizer is prepared using following methods:
(1) mineral powder body material (humic acid, carbonatoapatite, niter, shell, magnesium silicate, perlite and clay) is used It is raw material with trapping agent hydroxypropyl methyl cellulose, prepares mixed mineral particulate vector；
(2) ultra high efficiency nitrogen-fixing bacteria, phosphate solubilizing bacteria and potassium solubilizing bacteria are inoculated in first cell culture medium respectively to cultivate, obtain level-one Strain；
First class inoculum is inoculated in secondary medium respectively to cultivate, obtains second class inoculum；
Second class inoculum is inoculated in fermentation medium respectively to cultivate, obtains fermentation liquid；The fermentation liquid is efficiently solid The mother liquor of nitrogen bacterium thallus, phosphate solubilizing bacteria thallus and potassium solubilizing bacteria thallus；
(3) fermentation liquid of high-efficiency nitrogen-fixing bacterium, phosphate solubilizing bacteria and potassium solubilizing bacteria is uniformly mixed, mixed mineral particulate vector is carried out It is spraying, it is adsorbed in ultra high efficiency nitrogen-fixing bacteria, phosphate solubilizing bacteria and potassium solubilizing bacteria in mixed mineral particulate vector, obtains mandarin tree special fertilizer Material, the mandarin tree dedicated fertilizer are the characteristic natural green fertilizer without synthesis ammonia, phosphorus compound and potassium compound.
In some embodiments of the invention, the ingredient of the bactericidal suspending agent of the first Thief zone type is as follows: antibacterial peptide 1 ~5%, chlorogenic acid 1~5%, the gloomy copper 10~40% of thiophene, white carbon black 1~5%, diatomite 1~5%, lauryl sodium sulfate 1~ 3%, pull open powder 1~3%, sodium lignin sulfonate 1~5% and maleic acid di-sec-octyl 1~3%, remaining is deionized water. Using antibacterial peptide, chlorogenic acid and the gloomy copper of thiophene as effective component in the first bactericidal suspending agent of Thief zone type, high-efficient penetrant is added Maleic acid di-sec-octyl in medicament rapid osmotic to plant tissue, kills pathogenetic bacteria.
In some embodiments of the invention, by antibacterial peptide 1~5%, chlorogenic acid 1~5%, the gloomy copper 10~40% of thiophene is white Carbon black 1~5%, diatomite 1~5%, lauryl sodium sulfate 1~3%, pull open powder 1~3%, sodium lignin sulfonate 1~5% It with maleic acid di-sec-octyl 1~3%, is dissolved in deionized water, is killed carefully through sand mill attrition process at the first Thief zone type Bacterium suspending agent；
The first bactericidal suspending agent of Thief zone type is diluted with water 500-750 times and is made into aqueous suspensions, spraying to use.
In some embodiments of the invention, the ingredient of the second Thief zone type insecticidal suspending agent is as follows: clothianidin 5~ 20%, Buprofezin 10~30%, flonicamid 5~20%, white carbon black 1~5%, diatomite 1~5%, dodecyl sulphate Sodium 1~3%, pull open powder 1~3%, maleic acid di-sec-octyl 1~3%, remaining is deionized water.In the second Thief zone type Using clothianidin, Buprofezin and flonicamid as effective component in insecticidal suspending agent, high-efficient penetrant maleic acid two is added Secondary monooctyl ester is conducive to medicament rapid osmotic to plant tissue and insect body wall, so that eliminating Citrus Huanglongbing pathogen passes malicious insect vector Wood louse.
In some embodiments of the invention, by clothianidin 5~20%, Buprofezin 10~30%, flonicamid 5~ 20%, white carbon black 1~5%, diatomite 1~5%, lauryl sodium sulfate 1~3%, pull open powder 1~3% and maleic acid Di-sec-octyl 1~3%, is dissolved in deionized water, through sand mill attrition process at the second Thief zone type insecticidal suspending agent；
Second Thief zone type insecticidal suspending agent is diluted with water 1000-3000 times and is made into aqueous suspensions, spraying to use.
The present invention is matched according to the preferred mineral material of demand of Ci trus growth supplement mineral element, by multi mineral powder Body material is modified through hydroxypropyl methyl cellulose capture, obtains the mixed mineral particle with porous structure, and is utilized mixed It closes mineral grain function bacterium is adsorbed in the particulate vector as carrier, plays applied once and meet whole year NPK nutrition The effect of demand；And microelement abundant is provided for citrus；The application of mixed mineral particle with porous structure is also Soil aggregate structure can be improved, conducive to the breeding of functional microorganism, the conversion of nutrient and activating soil, promote root growth, battalion It is balanced to support supply, improves the yield and quality of fruit.Relative to existing organic fertilizer and inorganic fertilizer, dose is few, every year It only applies 1 time, can meet the needs of mandarin tree whole year growth nutrient, save labor, no pollution to the environment.
The present invention is by being 20-40 parts of humic acid, 5-15 parts of carbonatoapatites, 5-15 parts of niters, 3-5 portions of shellfishes by component Shell, 5-15 part magnesium silicate, 5-15 parts of perlites and 5-20 parts of clays carry out being crushed to diameter 3.5-5mm, form natural mixing Mineral powder；It is another to contain 0.5-2 parts of hydroxypropyl methyl celluloses for trapping agent.By in natural mixed mineral powder input mixer, stirring 5% hydroxypropyl methyl cellulose aqueous solution of lower penetrating is mixed, powder mineral material is captured.Hydroxypropyl methyl cellulose is captured again Mineral material afterwards is transported to pelletizer granulation, and by particle in 150-200 DEG C of dryness finalization, quenching to room temperature is made Mixed mineral particle containing various nutrient elements and porous structure.The adsorption function bacterium on above-mentioned particulate vector, containing useful natural The ultra high efficiency nitrogen-fixing bacteria (SDTB-0035) of bacillus azotobacter breeding, fixed nitrogen level improve 70.48 times compared with Natural strains；With drop The phosphate solubilizing bacteria (SDTB-0043) for solving the breeding of organophosphorus pesticide bacillus, directly can be changed into water for the phosphorus in carbonatoapatite powder Soluble phosphorus；It, can will be in kentite mountain flour with the potassium solubilizing bacteria (SDTB-0052) of the bacillus breeding of degradation carbamate chemicals for agriculture Potassium becomes water-soluble potassium, particulate vector is granulated into natural minerals materials such as humic acid, mixed mineral particle and clays, by function Bacterium is adsorbed in carrier the characteristic natural green fertilizer become without synthesis ammonia, phosphorus compound and potassium compound (chemical fertilizer).The fertilizer Material can obtain nitrogen, phosphorus decomposing and potassium decomposing by ultra high efficiency nitrogen-fixing bacteria according to citrus to nutrient demands such as nitrogen, phosphorus, potassium from atmosphere Bacterium dissociates rapid available phosphorus and available potassium, mixed mineral particle and humic from the minerals being rich in mixed mineral particle and soil The organic matter carriers such as acid and soil can provide middle microelement, meet the nutrition supply of citrus fruit trees comprehensively, which can be complete Full substitution ammonia, phosphorus compound and potassium compound (chemical fertilizer) organic fertilizer and all fertilizer, moreover, the mixed mineral The porous structure that grain has can guarantee the loose of soil, reduce soil aciditiy and salinity, improve the content of organic matter, improves and optimizes Soil microenvironment creates the most suitable Soil Ecological Condition of Ci trus growth, promotes root growth, keeps Growth status strong It is strong.It can be seen that above-mentioned improved fertilizer plays improvement soil, reduces soil aciditiy and salinity, optimizes soil microenvironment Effect promotes root growth, makes oranges and tangerines tree body robust growth, disease resistance is strong.
Meanwhile the ingredient that the medicament of Citrus Huanglongbing pathogen is prevented and treated in the present invention is as follows: antibacterial peptide 1~5%, chlorogenic acid 1~ 5%, white carbon black 1~5%, diatomite 1 is added using antibacterial peptide, chlorogenic acid and the gloomy copper of thiophene as effective component in the gloomy copper 10~40% of thiophene ~5%, lauryl sodium sulfate 1~3%, pull open powder 1~3%, sodium lignin sulfonate 1~5%, maleic acid di-sec-octyl 1~3%, remaining is deionized water, and attrition process is sanded into the first bactericidal suspending agent of Thief zone type.The Thief zone type kills carefully Bacterium suspending agent is diluted with water spraying use.Using antibacterial peptide, chlorogenic acid and the gloomy copper of thiophene, as the composition of effective component, there is not been reported, It is processed into the first bactericidal suspending agent of Thief zone type and adds water spray, is conducive to medicament rapid osmotic to plant tissue, kills Internal pathogenic bacteria.
Meanwhile the ingredient that the malicious insect vector medicament of Citrus Huanglongbing pathogen biography is eliminated in the present invention is as follows: clothianidin 5~20%, Buprofezin 10~30%, flonicamid 5~20% are added white using clothianidin, Buprofezin and flonicamid as effective component Carbon black 1~5%, diatomite 1~5%, lauryl sodium sulfate 1~3%, pull open powder 1~3%, maleic acid di-sec-octyl 1 ~3%, remaining is deionized water, and attrition process is sanded into the second Thief zone type insecticidal suspending agent composition.This is second hypertonic Saturating type insecticidal suspending agent is diluted with water spraying use.Clothianidin, Buprofezin and flonicamid be effective component composition still It has not been reported, it is processed into the second Thief zone type insecticidal suspending agent and adds water spray, is conducive to medicament rapid osmotic to plant group It knits and insect body wall, kill wood louse or accelerates death by a large amount of medicaments of wood louse piercing and sucking citrus tissue resorption, to eliminate Citrus Huanglongbing pathogen passes malicious insect vector.
Specific embodiment
To make the objectives, technical solutions, and advantages of the present invention clearer, below in conjunction with specific embodiment, to this hair Bright further description.
Ashby involved in following embodiment (A Xubei) nitrogen-free agar: mannitol 10g, CaCO35.0g, MgSO4· 7H2O 0.2g, KH2PO40.2g, CaSO4·2H2O 0.1g, NaCl 0.2g, distilled water are settled to 1L, pH7.0, agar powder 20g (fluid nutrient medium is not added), 121 DEG C of sterilizing 20min.
Pikovskaya culture medium: yeast extract 0.5g, glucose 10g, NaCl 0.3g, KCl 0.3g, MgSO4·7H2O 0.3g, MnSO4·H2O 0.03g, FeSO4·7H2O 0.03g, (NH4)2SO40.5g, Ca3(PO4)25.0g, distilled water constant volume To 1L, pH 7.0~7.5, agar powder 20g (fluid nutrient medium is not added), 121 DEG C of sterilizing 20min.
Alexandria Bao Luofu culture medium: sucrose 3g, potassium pin mountain flour (wash five times) 1g, MgSO with deionized water4·7H2O 0.5g, Na2PO42g, FeCl30.005g, CaCO30.1g, distilled water are settled to 1L, pH 7.0, agar 20g (Liquid Culture Base is not added), 121 DEG C of sterilizing 20min.
LB culture medium: peptone 10g, yeast extract 5g, NaCl 10g, distilled water are settled to 1L, pH7.0, agar powder 20g (fluid nutrient medium is not added), 121 DEG C of sterilizing 20min.
The screening of 1 ultra high efficiency nitrogen-fixing bacteria of embodiment
The primary dcreening operation of 1.1 nitrogen-fixing bacteria
(1) it prepares sample diluting liquid: weighing the soil-like for being derived from the planting vegetable for years in Tai'an area, Taian Shandong city Product 10g is fitted into the conical flask of 250mL, and appropriate bead and 100mL sterile water is added, and after being standing and soaking 20min, is shaken in constant temperature 200rpm vibrates 30min in bed, forms bacteria suspension mother liquor, is then successively diluted with sterile water, obtain 10-5、10-6、10-7Dilution The sample solution of degree.
(2) inverted plate: 60 DEG C are cooled to after the sterilizing of A Xubei nitrogen-free solid medium, pour plate.
(3) spread plate: 10-5、10-6、10-7The sample solution of dilution respectively draws 100 μ L, is scraped respectively with glass triangle It is uniform in culture medium coating.
(4) it cultivates: being inverted into 37 DEG C of constant temperature biochemical cultivation cases and train after the plate that coating finishes is sealed with sealed membrane Support 7d.
(5) primary dcreening operation: media surface picks out nitrogen-fixing bacteria from plate, then using the method for plate streaking in A Xubei It is isolated and purified in nitrogen-free solid medium, purifying bacterial strain is inoculated into LB slant medium respectively, is identified through nitrogen fixing capacity Being confirmed as Natural Nitrogen Fixation Strain Designation is SDTB-0001,4 DEG C of preservations in refrigerator.
The secondary screening of 1.2 nitrogen-fixing bacteria
(1) the azotobacter strain SDTB-0001 that primary dcreening operation obtains inoculation activation: is inoculated into Ah 's shellfish nitrogen-free fluid nutrient medium In, 37 DEG C of 200rpm shaken cultivations in constant-temperature table.
(2) nitrogen fixing capacity measures: taking one time fermentation liquid every 8h, using the culture solution of not inoculating strain as control group, uses Micro-kjoldahl method (kjeldahl apparatus) measures nitrogen pool in corresponding fermentation liquid.The bacterial strain that secondary screening is obtained is with 20% glycerol It is placed in -20 DEG C of preservations in refrigerator.
1.3 ultra high efficiency nitrogen-fixing bacteria domestication breedings
It is inoculated into the training of the LB liquid in 250 milliliters of triangular flasks respectively from one ring bacterium of picking each in SDTB-0001 with oese Base is supported, 1 milliliter of liquid nitrogen is added after sealing, 37 DEG C in shaking table, 200rpm shaken cultivation domestication breeding, switching time in every 15 days, even Continuous domestication breeding 120 times.Then in culture triangular flask 25cm overhung 20W ultraviolet lamp, irradiation 20 minutes, strengthen mutagenesis daily Effect continues continuous domestication breeding 30 times, obtains ultra high efficiency strains of nitrogen-fixing, is named as SDTB-0035.
The colony morphological observation of 1.4 nitrogen-fixing bacteria
With oese, each one ring ultra high efficiency nitrogen-fixing bacteria (SDTB-0035) of picking are inoculated into the training of LB liquid from the inclined-plane of preservation Base is supported, 37 DEG C in shaking table, 200rpm shaken cultivation to logarithmic phase observes microbial morphology under an optical microscope.Then it chooses Take fermentation liquid, in LB plate streaking, Yu Hengwen biochemical cultivation case 37 DEG C culture, observation colony shape, gloss, color, size, Situations such as transparency and chromogenic element.
2. the selection result of nitrogen-fixing bacteria primary dcreening operation
By isolate 10 plants of Natural Nitrogen Fixation bacterium, 1 plant of stronger bacterium of nitrogen fixing capacity is filtered out by measuring amount of nitrogen fixation Strain, is named as SDTB-0001.
3. high-efficiency nitrogen-fixing bacterium domestication breeding result
With oese, each one ring bacterium of picking is inoculated into 250 milliliters of triangular flasks respectively from Natural Nitrogen Fixation bacterium (SDTB-0001) In LB liquid medium, after sealing be added 1 milliliter of liquid nitrogen, 37 DEG C in shaking table, 200rpm shaken cultivation domestication breeding, every 15 Its switching time continuous domestication breeding 120 times, then hangs 20W ultraviolet lamp, daily irradiation 2 hours on culture triangular flask 25cm, Mutagenesis intensity is improved, is continued continuous domestication breeding 30 times, is obtained 1 plant of ultra high efficiency strains of nitrogen-fixing, be named as SDTB-0035.
Nitrogen pool in corresponding fermentation liquid is measured using Micro-kjoldahl method (kjeldahl apparatus), by fixed nitrogen domestication breeding The 1 plant of stronger bacterial strain of nitrogen fixing capacity obtained, is named as SDTB-0035.The nitrogen fixing capacity for screening naturalized strain is as shown in the table:
The nitrogen fixing capacity of the screening naturalized strain of table 1
Bacterial strain Amount of nitrogen fixation (mg/L)
Ultra high efficiency nitrogen-fixing bacteria (SDTB-0035) 835.48
Natural Nitrogen Fixation bacterium (SDTB-0001) 11.85
CK 0
Wherein, CK refers to blank control (not connecing the culture solution of bacterial strain), and Natural Nitrogen Fixation bacterium is this laboratory screening and protects The bacterial strain SDTB-0001 of the Natural Nitrogen Fixation bacterium of hiding.As shown in Table 1, the ultra high efficiency nitrogen-fixing bacteria (SDTB-0035) of domestication breeding, Gu Nitrogen level improves 70.48 times compared with Natural Nitrogen Fixation bacterium (SDTB-0001).
The colony diameter that the ultra high efficiency nitrogen-fixing bacteria (SDTB-0035) that domestication breeding obtains are formed on LB culture medium is about 1- 2mm, bacterium colony are round, white, opaque, center projections, rough surface.And the ultra high efficiency nitrogen-fixing bacteria for obtaining domestication breeding (SDTB-0035) commission Shanghai Sheng Gong bioengineering Co., Ltd carries out gene sequencing.The 16SrDNA sequence of the bacterium is submitted to Into GENBANK database carry out BLAST compare analysis, analysis the result shows that, the gene order and Xie Dian of the high-efficiency nitrogen-fixing bacterium The gene order very high homology of afnyloliquefaciens, homology is up to 99%.
Combining form and 16SrDNA sequencing result, the ultra high efficiency nitrogen-fixing bacteria that domestication breeding is obtained are named as SDTB- 0035, classification naming is bacillus amyloliquefaciens (Bacillus amyloliquefaciens), is preserved in China Microbiological bacterium Kind preservation administration committee common micro-organisms center, preservation address is Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, preservation day Phase is on April 26th, 2019, and deposit number is CGMCC NO.17635.
The screening of 2 phosphate solubilizing bacteria of embodiment
The screening of 1.1 phosphate solubilizing bacterias
Screening technique is as follows:
(1) it prepares sample diluting liquid: weighing to pick up from and be adopted from waste water treatment plant, Huayang Agricultural Chemial Group Co Ltd, Shandong Collection activated sludge sample 10g is fitted into the conical flask of 250mL, and appropriate bead and 100mL sterile water is added, is standing and soaking After 20min, 200rpm vibrates 30min in constant-temperature table, forms bacteria suspension mother liquor, is then successively diluted, obtained with sterile water 10-5、10-6、10-7The sample solution of dilution.
(2) inverted plate: 60 DEG C are cooled to after the sterilizing of Pikovskaya solid medium, pour plate.
(3) spread plate: 10-5、10-6、10-7The sample solution of dilution respectively draws 100 μ L, is scraped respectively with glass triangle It is uniform in culture medium coating.
(4) it cultivates: being inverted into 37 DEG C of constant temperature biochemical cultivation cases and train after the plate that coating finishes is sealed with sealed membrane Support 7d.
(5) primary dcreening operation: picking out the bacterium colony with Soluble phosphorus circle from Pikovskaya solid medium tablets, then using flat The method of plate scribing line is isolated and purified in Pikovskaya solid medium, and the purifying bacterial strain for having Soluble phosphorus circle phenomenon is distinguished It is inoculated into LB slant medium, 4 DEG C of preservations in refrigerator.
(6) mutagenic and breeding: the significant bacterial strain of Soluble phosphorus circle phenomenon is coated on Pikovskaya solid medium, plate It is inverted into after being sealed with sealed membrane in 37 DEG C of constant temperature biochemical cultivation cases, 20W ultraviolet lamp is hung on plate, daily 20 points of irradiation Clock improves mutagenesis intensity, cultivates 7d.By the most significant strain inoculated of Soluble phosphorus circle phenomenon to LB slant medium, 4 DEG C in refrigerator It saves.
The colony morphological observation of 1.2 phosphate solubilizing bacterias
With oese, each one ring bacterium of picking is inoculated into LB liquid medium from the inclined-plane of preservation, 37 DEG C in shaking table, 200rpm shaken cultivation observes microbial morphology to logarithmic phase under an optical microscope.Then picking fermentation liquid is drawn in LB plate Line, 37 DEG C of cultures in Yu Hengwen biochemical cultivation case, the feelings such as observation colony shape, gloss, color, size, transparency and chromogenic element Condition.
Degradation of 1.3 phosphate solubilizing bacterias to organophosphorus pesticide
By the seed liquor of phosphate solubilizing bacteria that screening obtains according to 5% inoculum concentration be inoculated in respectively containing phoxim, omethoate or In the selection culture medium of parathion, culture is added in organophosphorus pesticide in the form of organophosphorus pesticide solution in Selective agar medium Base, organophosphorus pesticide content is 35% in organophosphorus pesticide solution, and the additive amount of organophosphorus pesticide solution is in Selective agar medium 2 ‰ (mass ratioes)；Selective agar medium other compositions in addition to organophosphorus pesticide solution are 0.1g NaCl, 0.5g MgSO4, 0.3g K2SO4, 0.1gCaCO3, 0.001g FeSO4, 1000mL H2O, pH value are adjusted to 7.2, be placed in temperature be 37 DEG C, revolving speed 200rpm Environment in vibrate 48h, be then gradually increased the additive amount of 3 kinds of organophosphorus pesticide solution again, respectively additive amount be 4 ‰ (matter Amount ratio), 6 ‰ (mass ratioes), the upgrowth situation that each bacterial strain is observed under 8 ‰ (mass ratioes).Shaken cultivation 48h, gas Chromatographic Determination Degradation rate of the phosphate solubilizing bacteria to organophosphorus pesticide, determination condition are as follows: chromatographic column: silicon chromatographic column [DB-5MS, 30m (length) × 0.25mm (internal diameter) × 0.25 μm (film thickness)], sample volume: 1 μ L；Using dividing technology after column, split ratio 1: 10；Carrier gas: high-purity helium, Constant voltage mode: 91kPa；Injector temperature: 280 DEG C；Column oven temperature program: furnace temperature is in 70 DEG C of holding 2min after sample introduction, with 5 DEG C/speed of min is warming up to 290 DEG C, 3min is kept, then cool to 70 DEG C.
2. the selection result of phosphate solubilizing bacteria
By primary dcreening operation and ultraviolet mutagenesis, 5 plants of phosphate solubilizing bacterias are filtered out altogether, by measuring the degradation rate to organophosphorus pesticide, choosing Select phosphorus decomposing effect preferably and the highest bacterial strain of degradation efficiency, be named as phosphate solubilizing bacteria (SDTB-0043), gas Chromatographic Determination result table Bright, phosphate solubilizing bacteria (SDTB-0043) is respectively 73.1% to the degradation rate of organophosphorus pesticide phoxim, omethoate or parathion, 72.4% and 74.2%.
Screening the colony diameter that obtained phosphate solubilizing bacteria (SDTB-0043) is formed on LB culture medium is about 1-2mm, and bacterium colony is Round, white, opaque, center projections, rough surface.And the phosphate solubilizing bacteria (SDTB-0043) for obtaining screening entrusts Hua Da base Because company carries out gene sequencing.The 16SrDNA sequence of the bacterium is submitted in GENBANK database and carries out BLAST comparison point Analysis, analysis the result shows that, the gene order very high homology of the strain sequence and bacillus amyloliquefaciens, homology is up to 99%.
Combining form and 16SrDNA sequencing result, the phosphate solubilizing bacteria that screening is obtained are named as SDTB-0043, classification life Entitled bacillus amyloliquefaciens (Bacillus amyloliquefaciens) are preserved in Chinese microorganism strain preservation management committee Member's meeting common micro-organisms center, preservation address is Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, and the deposit date is in April, 2019 26, deposit number was CGMCC NO.17637.
Degradation of 3 phosphate solubilizing bacteria of embodiment (SDTB-0043) to carbonatoapatite powder
The seed liquor for the phosphate solubilizing bacteria (SDTB-0043) that screening obtains is inoculated according to 5% inoculum concentration containing carbonatoapatite In the selection culture medium of powder, culture medium is added in carbonatoapatite powder in the form of carbonatoapatite powder solution in Selective agar medium, Carbonatoapatite powder content is 35% in carbonatoapatite powder solution, and the additive amount of carbonatoapatite powder solution is 8 ‰ in Selective agar medium (mass ratio)；Selective agar medium other compositions in addition to organophosphorus pesticide solution are 0.1g NaCl, 0.5g MgSO4, 0.3g K2SO4, 0.1gCaCO3, 0.001g FeSO4, 1000mL H2O, pH value are adjusted to 7.2, be placed in temperature be 37 DEG C, revolving speed 200rpm Environment in vibrate 48h, using phosphomolybdic acid blue laws survey fermentation liquid in titanium pigment content.
The measuring method of titanium pigment content:
2mL fermentation liquid is drawn under aseptic condition, 4000rpm is centrifuged 10min, draws 1mL supernatant, and dilute 10 times.It inhales 2mL ammonium molybdate solution is added in supernatant after taking 2mL to dilute, mixes, and stands.1mL anhydrous sodium sulfite solution is added, mixes, Add 1mL quinol solution mixing.Add distilled water to 20mL, mixes, boil 10min.With nonvaccinated after sterilizing Pikovskaya fluid nutrient medium is control group.With absorbance value of the spectrophotometer sample at 660nm.Utilize phosphorus standard Curve calculates phosphorus content in sample.Phosphorus content is calculated with formula:
X=m × 10/V
X: phosphorus content in fermentation liquid, mg/mL
M: phosphorus content in the dilution obtained using phosphorus standard curve, mg
V: the volume of the supernatant after the dilution of absorption, mL
The measurement result of phosphomolybdic acid blue laws shows that the phosphorus decomposing amount of phosphate solubilizing bacteria (SDTB-0043) is 184.24mg/L.
It, can directly will be in carbonatoapatite powder with the phosphate solubilizing bacteria (SDTB-0043) of degrading organic phosphor pesticides bacillus breeding Phosphorus be changed into water-soluble phosphorus.
The screening of 4 potassium solubilizing bacteria of embodiment
The screening of 1.1 potassium solubilizing bacterias
Screening technique is as follows:
(1) it prepares sample diluting liquid: weighing to acquire from waste water treatment plant, Huayang Agricultural Chemial Group Co Ltd, Shandong and live Property mud sample 10g is fitted into the conical flask of 250mL, and appropriate bead and 100mL sterile water is added, after being standing and soaking 20min, 200rpm vibrates 30min in constant-temperature table, forms bacteria suspension mother liquor, is then successively diluted with sterile water, obtain 10-5、10-6、 10-7The sample solution of dilution.
(2) inverted plate: 60 DEG C are cooled to after the sterilizing of Alexandria Bao Luofu solid medium, pour plate.
(3) spread plate: 10-5、10-6、10-7The sample solution of dilution respectively draws 100 μ L, is scraped respectively with glass triangle It is uniform in culture medium coating.
(4) it cultivates: being inverted into 37 DEG C of constant temperature biochemical cultivation cases and train after the plate that coating finishes is sealed with sealed membrane Support 7d.
(5) primary dcreening operation: the bacterium colony with molten potassium circle is picked out from the Bao Luofu solid medium tablets of Alexandria, is then adopted It is isolated and purified in the Bao Luofu solid medium of Alexandria with the method for plate streaking, will there is the purifying of molten potassium circle phenomenon Bacterial strain is inoculated into LB slant medium respectively, 4 DEG C of preservations in refrigerator.
(6) mutagenic and breeding: the bacterium colony with molten potassium circle will be selected, using the method for plate streaking in Alexandria Bao Luofu In solid medium, 20W ultraviolet lamp is hung on plate, daily irradiation 20 minutes, improve mutagenesis intensity, cultivate 7d.By molten potassium The most significant strain inoculated of phenomenon is enclosed to LB slant medium, is saved for 4 DEG C in refrigerator.
The colony morphological observation of 1.2 potassium solubilizing bacterias
With oese, each one ring bacterium of picking is inoculated into LB liquid medium from the inclined-plane of preservation, 37 DEG C in shaking table, 200rpm shaken cultivation observes microbial morphology to logarithmic phase under an optical microscope.Then picking fermentation liquid is drawn in LB plate Line, 37 DEG C of cultures in Yu Hengwen biochemical cultivation case, the feelings such as observation colony shape, gloss, color, size, transparency and chromogenic element Condition.
Degradation of 1.3 potassium solubilizing bacterias to carbamates chemicals for agriculture such as sevins
The seed liquor for the potassium solubilizing bacteria that screening obtains is inoculated in respectively according to 5% inoculum concentration containing 100mg/L, 200mg/ L, in the LB liquid medium of 400mg/L sevin, 30mL/250mL conical flask is dispensed, is placed in that temperature is 37 DEG C, revolving speed is 72h is vibrated in the environment of 200rpm, observes the upgrowth situation of each bacterial strain；Shaken cultivation 72h, medium centrifugal take supernatant, on Clear liquid passes through degradation rate of the liquid chromatogram measuring potassium solubilizing bacteria to carbamates chemicals for agriculture such as sevins, determination condition: chromatographic column For WondaSil C18 column (150mm × 4.60mm, 5.0 μm)；Mobile phase is acetonitrile: water (85:15, V/V), flow velocity 0.5mL/ min；UV detector, wavelength 220nm；10 μ L of sample volume.
2. the selection result of potassium solubilizing bacteria
4 plants of potassium solubilizing bacterias are filtered out altogether by primary dcreening operation and ultraviolet mutagenesis, by measuring to the carbamates agriculture such as sevin The degradation rate of medicine selects potassium decomposing effect preferably and the highest bacterial strain of degradation efficiency, is named as potassium solubilizing bacteria (SDTB-0052), liquid phase Chromatographic determination the result shows that, potassium solubilizing bacteria (SDTB-0052) is 84.3% to carbamates chemicals for agriculture such as sevins.
Screening the colony diameter that obtained potassium solubilizing bacteria (SDTB-0052) is formed on LB culture medium is about 1-2mm, and bacterium colony is Round, white, opaque, center projections, rough surface.And the potassium solubilizing bacteria (SDTB-0052) for obtaining screening entrusts Hua Da base Because company carries out gene sequencing.The 16SrDNA sequence of the bacterium is submitted in GENBANK database and carries out BLAST comparison point Analysis, analysis the result shows that, the gene order very high homology of the strain sequence and bacillus amyloliquefaciens, homology is up to 99%.
Combining form and 16SrDNA sequencing result, the potassium solubilizing bacteria that screening is obtained are named as SDTB-0052, classification life Entitled bacillus amyloliquefaciens (Bacillus amyloliquefaciens) are preserved in Chinese microorganism strain preservation management committee Member's meeting common micro-organisms center, preservation address is Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, and the deposit date is in April, 2019 26, deposit number was CGMCC NO.17639.
Degradation of 5 potassium solubilizing bacteria of embodiment (SDTB-0052) to kentite mountain flour
The seed liquor for the potassium solubilizing bacteria (SDTB-0052) that screening obtains is inoculated in mountain flour containing kentite according to 5% inoculum concentration LB liquid medium in, in LB liquid medium the additive amount of kentite mountain flour be 400mg/L, be placed in temperature be 37 DEG C, revolving speed To vibrate 72h in the environment of 200rpm, culture solution 4000rpm is centrifuged 10min, takes supernatant flame spectrophotometer (rib light FP6410 formula) measure Content of soluble potassium in fermentation liquid.
Flame spectrophotometer measurement result shows that potassium decomposing amount is 34.78mg/L.
It, can be by kentite mountain flour with the potassium solubilizing bacteria (SDTB-0052) of the bacillus breeding of degradation carbamate chemicals for agriculture In potassium become water-soluble potassium.
The preparation of 6 mandarin tree dedicated fertilizer of embodiment
Mandarin tree dedicated fertilizer in the present embodiment includes function bacterium and mixed mineral particulate vector；Function bacterium is adsorbed in institute It states in mixed mineral particulate vector, function bacterium includes ultra high efficiency nitrogen-fixing bacteria SDTB-0035, phosphate solubilizing bacteria SDTB-0043 and potassium solubilizing bacteria SDTB-0052；
The mixed mineral particulate vector includes each component of following parts by weight: by 30kg humic acid, 10kg carbonatoapatite, 10kg niter, 10kg shell, 10kg magnesium silicate, 10kg perlite, 19kg clay and 1kg trapping agent hydroxypropyl methyl fiber Element.
In the present embodiment, the preparation method of mandarin tree dedicated fertilizer, comprising the following steps:
The preparation of A mixed mineral particulate vector
(1) by 30kg humic acid, 10kg carbonatoapatite, 10kg niter, 10kg shell, 10kg magnesium silicate, 10kg pearl Rock and 19kg clay carry out being crushed to diameter 3.5-5mm, form natural mixed mineral powder；
(2) 1kg hydroxypropyl methyl cellulose aqueous solution is configured to the hydroxypropyl methyl cellulose water that mass concentration is 5% Solution,
By in natural mixed mineral powder input mixer, it is fine that the hydroxypropyl methyl that mass concentration is 5% is sprayed under stiring Plain aqueous solution is tieed up, (includes: humic acid, carbonatoapatite, niter, shell, magnesium silicate, perlite and glutinous by mineral powder body material Soil) capture；
(3) mineral material after capturing hydroxypropyl methyl cellulose is transported to pelletizer granulation, and by mixed mineral Grain dryness finalization, quenching to room temperature at 150 DEG C, are made the mixed mineral containing various nutrient elements and porous structure Grain.
The preparation of B ultrasound high-efficiency nitrogen-fixing bacterium SDTB-0035, phosphate solubilizing bacteria SDTB-0043 and potassium solubilizing bacteria SDTB-0052
Culture medium: starch 100g/L, beancake powder 100g/L, dipotassium hydrogen phosphate 2g/L, ammonium sulfate 1g/L, magnesium sulfate 0.5g/ L, chlorination 0.01g/L, calcium carbonate 0.1g/L, yeast extract 0.1g/L, pH7.2.
Respectively by ultra high efficiency nitrogen-fixing bacteria SDTB-0035, phosphate solubilizing bacteria SDTB-0043 and potassium solubilizing bacteria SDTB-0052 actication of culture It accesses in the above-mentioned culture medium of 1L afterwards, triangular flask shaking flask 200rpm, 37 DEG C are cultivated 24 hours, are then inoculated by 1% inoculum concentration In 250L seed fermentation tank, ventilation, 37 DEG C are cultivated 24 hours, then are connected in 2500L fermentor by 10% inoculum concentration, 37 DEG C of cultures 24 hours, stops fermentation after 80% thallus generates gemma, obtain fermentation liquid；According to 100L fermentation liquid production 1000kg bacterial manure Ratio determines the volume of fermentation liquid.
The preparation of C mandarin tree dedicated fertilizer
By the fermentation liquid of ultra high efficiency nitrogen-fixing bacteria SDTB-0035, phosphate solubilizing bacteria SDTB-0043 and potassium solubilizing bacteria SDTB-0052 thallus It is uniformly mixed, is 1:10 according to the mass ratio of fermentation liquid and mixed mineral particulate vector, mixed mineral particulate vector is sprayed Mist makes ultra high efficiency nitrogen-fixing bacteria SDTB-0035, phosphate solubilizing bacteria SDTB-0043 and potassium solubilizing bacteria SDTB-0052 thallus be adsorbed in mixed mineral In particulate vector, mandarin tree dedicated fertilizer is obtained.
The preparation of 7 mandarin tree dedicated fertilizer of embodiment
The present embodiment and the difference of embodiment 6 be, the formula of mixed mineral particulate vector are as follows: 20kg humic acid, 15kg Carbonatoapatite, 15kg niter, 8kg shell, 12kg magnesium silicate, 12kg perlite and 17kg clay；Step (3) is also different, Specifically, the mineral material after (3) capture hydroxypropyl methyl cellulose is transported to pelletizer granulation, and by mixed mineral particle The mixed mineral particle containing various nutrient elements and porous structure is made in the dryness finalization at 200 DEG C, quenching to room temperature； Remaining step is same as Example 6.
The preparation of 8 mandarin tree dedicated fertilizer of embodiment
The present embodiment and the difference of embodiment 6 be, the formula of mixed mineral particulate vector are as follows: by 40kg humic acid, 7kg Carbonatoapatite, 7kg niter, 10kg shell, 10kg magnesium silicate, 10kg perlite and 15kg clay；Step (3) is also different, Specifically, the mineral material after (3) capture hydroxypropyl methyl cellulose is transported to pelletizer granulation, and by mixed mineral particle The mixed mineral particle containing various nutrient elements and porous structure is made in the dryness finalization at 180 DEG C, quenching to room temperature； Remaining step is same as Example 6.
Embodiment 9 prevents and treats the preparation of the medicament of Citrus Huanglongbing pathogen
The ingredient that the medicament of Citrus Huanglongbing pathogen is prevented and treated in the present embodiment is as follows: antibacterial peptide 3%, chlorogenic acid 3%, the gloomy copper of thiophene 24%, using antibacterial peptide, chlorogenic acid and the gloomy copper of thiophene as effective component, white carbon black 3%, diatomite 2%, lauryl sodium sulfate is added 2%, pull open powder 2%, sodium lignin sulfonate 2%, maleic acid di-sec-octyl 2%, remaining is deionized water；According to above-mentioned ratio Example is by antibacterial peptide, chlorogenic acid, the gloomy copper of thiophene, white carbon black, diatomite, lauryl sodium sulfate, pull open powder, sodium lignin sulfonate and suitable Butene dioic acid di-sec-octyl, is dissolved in deionized water, through sand mill attrition process at the first bactericidal suspending agent of Thief zone type.It will The first bactericidal suspending agent of Thief zone type is diluted with water 700 times and is made into aqueous suspensions, spraying to use.
Wherein, the metabolin that antibacterial peptide is generated by bacillus subtilis (Bacillus subtilis) fermentation, has sterilization It is acted on inducing plant resistance, the fermentation liquid of bacillus subtilis can be used directly, and can also therefrom extract active material antibacterial peptide Reprocessing uses afterwards.
Antibacterial peptide is isolated from the fermentation liquid of bacillus subtilis, and a kind of preparation of optional antibacterial peptide is provided below Method.
The preparation method of the antibacterial peptide, comprising the following steps:
1) bacterial strain activates
The intact inclined-plane bacillus subtilis of preservation is inoculated in slant medium, 35-37 DEG C of culture 16-20h carries out bacterium Kind activation；
2) liquid seeds culture
In seed culture medium after the access of activated bacillus subtilis slant strains is sterilized, in rotary shaker 180- 200r/min, 35-37 DEG C of constant-temperature shaking culture 16-20h, the bacteria containing amount of seed fermentation liquid are 107-108cfu/mL；
3) shake flask fermentation culture
The seed liquor that step 2) is obtained accesses triangular flask, the bottled shake flask fermentation of 1000mL triangle with the inoculum concentration of 4-10% Culture medium is 100-200mL, and triangle bottle stopper uses latex plug, 32-37 DEG C of fermentation temperature, revolving speed 160-200r/min shaking table culture 60-62h；
4) 15L gas formula Fermentation fermented and cultured
Gas-lifting type liquid deep layer fermenting: the canned fermentation medium 10L of 15L fermentation is carried out using fed batch fermentation mode, Fermentor is inoculated with according to 4-6% inoculum concentration after sterilization and cooling, by the shake flask fermentation liquid in step 3), fermentating controling condition are as follows: temperature 35-37 DEG C, time 60-62h, fermentation medium pH value 7.5-8.0 of degree, filtrated air ventilating ratio 1: 1 (v/v), tank pressure 0.02MPa ferments after 15-20h, adds glucose solution 100mL into fermentation liquid every 6-8h；
5) fermentation liquor pretreatment
Step 4) fermentation liquid 10000-12000r/min centrifugation 8-10min is removed into thallus, obtains fermented liquid supernatant.Take supernatant Liquid passes through the ultrafiltration membrane ultrafiltration that molecular cut off is 20kDa first, and the filtrate of collection is 1000Da's using molecular cut off Ultrafiltration membrane ultrafiltration, discards filtered solution, and antibacterial peptide solution is concentrated 10 times.The concentration antibacterial peptide solution that ultrafiltration obtains is heated to 82-85 DEG C, 32-35 DEG C is cooled the temperature to after keeping the temperature 12-15min, continues to be cooled to 4 DEG C of standings 4-6h, 10000-12000r/ Min centrifugation 8-10min removes the cotton-shaped suspension protein precipitation of heat denatured.At room temperature into the fermentation clear liquid after the above centrifugation Solid ammonium sulfate is slowly added into 20% saturation degree, after standing 4-6h in refrigerator, 1000-12000rmp is centrifuged 8-10min, collects Supernatant, then solid ammonium sulfate is added to saturation degree 80%, 4 DEG C of standings 4-6h, 1000-12000r/ into the supernatant of collection Min is centrifuged 8-10min, collects and precipitates and be dissolved in 0.02mol/L, in the Tris-HCl buffer of pH7.0, further using saturating Bag dialysis is analysed, therebetween every 3~4h, replaces 1 st pure water, must dialyse complete antibacterial peptide sample；
6) antibacterial peptide anion-exchange chromatography
By the complete antibacterial peptide sample loading DEAE-Sepharose FF anion-exchange column of step 5) dialysis.Using The Tris-HCl buffer solution of 0.02mol/L pH value 8.0 falls unbonded Impurity elution, is eluted to ultraviolet detection line always It does not change, then is carried out with the buffer solution of the Tris-HCl of the 0.02mol/L pH value 8.0 of the NaCl containing 0-1mol/L Linear gradient elution, elution speed 1.5ml/min are collected by every pipe 3ml, and the NaCl concentration that antibacterial peptide elutes is 0.50- 0.55mol/L.It is associated with the collecting pipe of antibacterial activity, after using molecular cut off to be concentrated by ultrafiltration for the cellulose membrane of 1000Da Anion-exchange chromatography active concentration liquid；
7) antibacterial peptide molecular sieve gel column filtration chromatography
By step 6) anion-exchange chromatography active concentration liquid loading Sephadex G-15 molecular sieve gel column, elution is slow Tris-HCl, the elution speed 1mL/min that solution is 0.02mol/L, pH value 8.0 are rushed, is collected by every pipe 2mL, is merged active Collecting pipe obtain molecular sieve gel filtration column chromatography antibacterial peptide living solution；
8) prepared by high-purity antibacterial peptide dry powder
Step 7) molecular sieve gel column filtration chromatography antibacterial peptide living solution is used to the ultrafiltration of molecular cut off 1000Da Film makes antibacterial peptide solution be concentrated 15 times for examination pathogen filtering.Then it is concentrated using rotavapor under vacuum, temperature 65-75 DEG C, when be concentrated into antibacterial peptide solution it is more sticky when, take out antibacterial peptide concentrate in -20 DEG C of freezing 8-10h, then by icing Antibacterial peptide is placed in vacuum freeze drier, and control vacuum degree is 30-50Pa, and temperature is -35 DEG C, drying time 15-18h, i.e., Obtain antibacterial peptide finished product.
The present embodiment produces antibacterial peptide using fermentation of bacillus subtilis, as long as the bacillus subtilis of antibacterial peptide can be generated Bacterium can prepare antibacterial peptide using the above method, and optionally, bacillus subtilis is bacillus subtilis BRT39 (CGMCC NO.5702), it is commercially available from China General Microbiological culture presevation administrative center, bacillus subtilis can also be used (Bacillus subtilis) mutation SDTB-0026, according to bacillus subtilis (Bacillus subtilis) mutation Its fermentation liquid can be used directly in SDTB-0026, reprocesses use after can also therefrom extracting active material antibacterial peptide.
Embodiment 10 prevents and treats the preparation of the medicament of Citrus Huanglongbing pathogen
The ingredient that the medicament of Citrus Huanglongbing pathogen is prevented and treated in the present embodiment is as follows: antibacterial peptide 2%, chlorogenic acid 2%, the gloomy copper of thiophene 26%, using antibacterial peptide, chlorogenic acid and the gloomy copper of thiophene as effective component, white carbon black 2%, diatomite 3%, lauryl sodium sulfate is added 2%, pull open powder 1%, sodium lignin sulfonate 3%, maleic acid di-sec-octyl 2%, remaining is deionized water；According to above-mentioned ratio Example is by antibacterial peptide, chlorogenic acid, the gloomy copper of thiophene, white carbon black, diatomite, lauryl sodium sulfate, pull open powder, sodium lignin sulfonate and suitable Butene dioic acid di-sec-octyl, is dissolved in deionized water, through sand mill attrition process at the first bactericidal suspending agent of Thief zone type.It will The first bactericidal suspending agent of Thief zone type is diluted with water 500 times and is made into aqueous suspensions, spraying to use.
Embodiment 11 prevents and treats the preparation of the medicament of Citrus Huanglongbing pathogen
The ingredient that the medicament of Citrus Huanglongbing pathogen is prevented and treated in the present embodiment is as follows: antibacterial peptide 2.5%, chlorogenic acid 2.5%, thiophene is gloomy White carbon black 2%, diatomite 3%, dodecyl sulphate is added using antibacterial peptide, chlorogenic acid and the gloomy copper of thiophene as effective component in copper 25% Sodium 2%, pull open powder 1%, sodium lignin sulfonate 3%, maleic acid di-sec-octyl 2%, remaining is deionized water；According to above-mentioned Ratio is by antibacterial peptide, chlorogenic acid, the gloomy copper of thiophene, white carbon black, diatomite, lauryl sodium sulfate, pull open powder, sodium lignin sulfonate and Maleic acid di-sec-octyl, is dissolved in deionized water, through sand mill attrition process at the first bactericidal suspending agent of Thief zone type. The first bactericidal suspending agent of Thief zone type is diluted with water 600 times and is made into aqueous suspensions, it is spraying to use.
Embodiment 12 eliminates the preparation that Citrus Huanglongbing pathogen passes the medicament of malicious insect vector
The ingredient that the medicament that Citrus Huanglongbing pathogen passes malicious insect vector is eliminated in the present embodiment is as follows: clothianidin 10%, thiazine White carbon black 3%, silicon is added using clothianidin, Buprofezin and flonicamid as effective component in ketone 20%, flonicamid 10% Diatomaceous earth 2%, lauryl sodium sulfate 2%, pull open powder 2%, maleic acid di-sec-octyl 2%, remaining is deionized water；According to Aforementioned proportion is by clothianidin, Buprofezin, flonicamid, white carbon black, diatomite, lauryl sodium sulfate, pull open powder, maleic Diacid di-sec-octyl, is dissolved in deionized water, through sand mill attrition process at the second Thief zone type insecticidal suspending agent.It is hypertonic by second Saturating type insecticidal suspending agent is diluted with water 3000 times and is made into aqueous suspensions, spraying to use.
In the present embodiment, each ingredient for eliminating the medicament that Citrus Huanglongbing pathogen passes malicious insect vector is commercially available.
Embodiment 13 eliminates the preparation that Citrus Huanglongbing pathogen passes the medicament of malicious insect vector
The ingredient that the medicament that Citrus Huanglongbing pathogen passes malicious insect vector is eliminated in the present embodiment is as follows: clothianidin 5%, Buprofezin 30%, white carbon black 3%, diatomite is added using clothianidin, Buprofezin and flonicamid as effective component in flonicamid 5% 2%, lauryl sodium sulfate 2%, pull open powder 2%, maleic acid di-sec-octyl 2%, remaining is deionized water；According to above-mentioned Ratio is by clothianidin, Buprofezin, flonicamid, white carbon black, diatomite, lauryl sodium sulfate, pull open powder, maleic acid Di-sec-octyl is dissolved in deionized water, through sand mill attrition process at the second Thief zone type insecticidal suspending agent.By the second Thief zone type Insecticidal suspending agent is diluted with water 1500 times and is made into aqueous suspensions, spraying to use.
Embodiment 14 eliminates the preparation that Citrus Huanglongbing pathogen passes the medicament of malicious insect vector
The ingredient that the medicament that Citrus Huanglongbing pathogen passes malicious insect vector is eliminated in the present embodiment is as follows: clothianidin 7.5%, thiazine White carbon black 3%, silicon is added using clothianidin, Buprofezin and flonicamid as effective component in ketone 25%, flonicamid 7.5% Diatomaceous earth 2%, lauryl sodium sulfate 2%, pull open powder 2%, maleic acid di-sec-octyl 2%, remaining is deionized water；According to Aforementioned proportion is by clothianidin, Buprofezin, flonicamid, white carbon black, diatomite, lauryl sodium sulfate, pull open powder, maleic Diacid di-sec-octyl, is dissolved in deionized water, through sand mill attrition process at the second Thief zone type insecticidal suspending agent.It is hypertonic by second Saturating type insecticidal suspending agent is diluted with water 2000 times and is made into aqueous suspensions, spraying to use.
The systematic treating method of Citrus Huanglongbing pathogen in the present embodiment, it is (real in citrus orchard over-all application mandarin tree dedicated fertilizer Apply the preparation of example 6,7 or 8), while the aqueous suspensions for continuously spraying the first bactericidal suspending agent of Thief zone type for disease plant (are implemented It is prepared by example 9,10 or 11) symptomatic treatment, sprays 1 time, continuous processing 3-4 times kills pathogenetic bacteria for 7-10 days；It is sent out in mandarin tree wood louse When raw, to the aqueous suspensions (preparation of embodiment 12,13 or 14) of the second Thief zone type insecticidal suspending agent of full tree sprinkling, eliminate and pass malicious matchmaker Jie insect.
The systematic treating method of Citrus Huanglongbing pathogen is created by above-mentioned 3 technical combinations.
Test case:
The present inventor is tried the systematic treating method of the Citrus Huanglongbing pathogen of the present embodiment on mandarin tree It tests, test result is as follows:
The present invention of example 1 Zhejiang Province's Huangyan City application attestation, in the tangerine garden that Citrus Huanglongbing pathogen is fragmentary, 2018 3 The moon 22, ring applies 300 kilograms of the mandarin tree dedicated fertilizer (preparation of embodiment 6) containing super nitrogen-fixing bacteria per acre；April 6 is in citrus Germination initial stage is diluted with water 600 times (in fact with the first bactericidal suspending agent of Thief zone type containing antibacterial peptide, chlorogenic acid and the gloomy copper of thiophene Apply the preparation of example 11) it uses by spraying, continuous sprinkling 4 times is spaced 7 days.By symptomatic treatment, pathogenetic bacteria is killed, makes tree body of falling ill It gets well growth.In wood louse early period of origination in 20 days or so May, contain clothianidin, Buprofezin and flonicamid 3 containing above-mentioned Second Thief zone type insecticidal suspending agent of kind insecticide is diluted with water 2000 times (preparations of embodiment 14) and uses by spraying.Eliminate mandarin orange The insect mediator diaphorina citri of tangerine yellow twig, prevents disease from propagating again.The tangerine garden treated through present system does not occur Huang Imperial disease symptoms, effective percentage are 100%.
The present invention of example 2 hazard prevention application attestation, in the granulated sugar orangery that Citrus Huanglongbing pathogen is fragmentary, 2018 3 The moon 15, ring applies 250 kilograms of the mandarin tree dedicated fertilizer (preparation of embodiment 7) containing super nitrogen-fixing bacteria per acre；April 12 is in citrus It is germination initial stage, (real with containing antibacterial peptide, chlorogenic acid and gloomy the first bactericidal suspending agent of bronze medal Thief zone type of thiophene, being diluted with water 700 times Apply the preparation of example 9) it uses by spraying, continuous sprinkling 3 times is spaced 10 days.By symptomatic treatment, pathogenetic bacteria is killed, makes tree body of falling ill It gets well growth.In wood louse early period of origination in 20 days or so May, contain clothianidin, Buprofezin and flonicamid 3 containing above-mentioned Second Thief zone type insecticidal suspending agent of kind insecticide is diluted with water 1500 times (preparations of embodiment 13) and uses by spraying.Eliminate mandarin orange The insect mediator diaphorina citri of tangerine yellow twig, prevents disease from propagating again.The granulated sugar orangery treated through present system does not occur Huanglong's disease symptoms, effective percentage are 100%.
The present invention is improved organic by reducing soil aciditiy and salinity in citrus orchard over-all application mandarin tree dedicated fertilizer Matter content improves and optimizes soil microenvironment, creates the most suitable Soil Ecological Condition of Ci trus growth, promotes root system raw It is long, keep Growth status healthy and strong.For occur Citrus Huanglongbing pathogen plant, with containing 3 kinds of antibacterial peptide, chlorogenic acid and the gloomy copper of thiophene effectively at The the first bactericidal suspending agent of Thief zone type divided, is diluted with water spraying use, continuously sprays symptomatic treatment, kills pathogen, makes Tree body is got well growth.Meanwhile in order to eliminate the insect mediator diaphorina citri of Citrus Huanglongbing pathogen, in its early period of origination, with containing There is the second Thief zone type insecticidal suspending agent of 3 kinds of clothianidin, Buprofezin and flonicamid insecticides, is diluted with water, makes by spraying With.The systematic treating method of Citrus Huanglongbing pathogen, good application effect are created by above-mentioned 3 technical combinations, morbidity mandarin tree obtains To healing.
The application by by 20-40 parts of humic acid of component, 5-15 parts of carbonatoapatites, 5-15 parts of niters, 3-5 parts of shells, 5-15 parts of magnesium silicates, 5-15 parts of perlites and 5-20 parts of clays carry out being crushed to diameter 3.5-5mm, form natural mixed mineral Powder；It is another to contain 0.5-2 parts of hydroxypropyl methyl celluloses for trapping agent.By in natural mixed mineral powder input mixer, under stiring 5% hydroxypropyl methyl cellulose aqueous solution is sprayed into, powder mineral material is captured.After hydroxypropyl methyl cellulose is captured again Mineral material is transported to pelletizer granulation, and by particle in 150-200 DEG C of dryness finalization, quenching to room temperature is made containing more The mixed mineral particle of kind nutrient and porous structure.The adsorption function bacterium on above-mentioned particulate vector contains useful Natural Nitrogen Fixation The ultra high efficiency nitrogen-fixing bacteria (SDTB-0035) of bacillus breeding, fixed nitrogen level improve 70.48 times compared with Natural strains；Have with degradation The phosphate solubilizing bacteria (SDTB-0043) of machine phosphorus insecticide bacillus breeding directly will can be changed into water-soluble phosphorus containing the phosphorus in ground phosphate rock；With The potassium solubilizing bacteria (SDTB-0052) of the bacillus breeding of degradation carbamate chemicals for agriculture, can will become containing the potassium in potassium breeze Water-soluble potassium is granulated into particulate vector with natural minerals materials such as humic acid, mixed mineral particle and clays, function bacterium is adsorbed Become the characteristic natural green fertilizer without synthesis ammonia, phosphorus compound and potassium compound (chemical fertilizer) in carrier.The fertilizer can root According to citrus to nutrient demands such as nitrogen, phosphorus, potassium, nitrogen, phosphorus decomposing and potassium solubilizing bacteria are obtained from atmosphere from mixed by ultra high efficiency nitrogen-fixing bacteria It closes the minerals being rich in mineral carrier and soil and dissociates rapid available phosphorus and available potassium, mixed mineral carrier in the miberal powder of addition It can provide middle microelement with the organic matter carriers such as humic acid and soil, meet the nutrition supply of fruit tree and crops comprehensively.It should Product can substitute ammonia, phosphorus compound and potassium compound (chemical fertilizer) organic fertilizer and all fertilizer completely, moreover, described mixed The porous structure that closing mineral grain has can guarantee the loose of soil, maintain the circulation of air, and not only be able to satisfy Orange Producing to feeding The needs divided, moreover it is possible to good quality and high output, improvement soil, it is environmental-friendly, realize the sustainable production of high-quality citrus, it is seen that above-mentioned improvement Fertilizer afterwards plays the effect of improvement soil, promotes root growth, makes oranges and tangerines tree body robust growth, disease resistance is strong.
Meanwhile the ingredient of the medicament of the prevention and treatment Citrus Huanglongbing pathogen in the application is as follows: antibacterial peptide 1~5%, chlorogenic acid 1~ 5%, white carbon black 1~5%, diatomite 1 is added using antibacterial peptide, chlorogenic acid and the gloomy copper of thiophene as effective component in the gloomy copper 10~40% of thiophene ~5%, lauryl sodium sulfate 1~3%, pull open powder 1~3%, sodium lignin sulfonate 1~5%, maleic acid di-sec-octyl 1~3%, remaining is deionized water, through sand mill attrition process at the first bactericidal suspension agent composition of Thief zone type.This first The bactericidal suspending agent of Thief zone type is diluted with water spraying use.And according to the retrieval of inventor, in the document of the prior art not See and uses antibacterial peptide, chlorogenic acid and the gloomy copper of thiophene for effective component to prepare Thief zone suspending agent and for treating Citrus Huanglongbing pathogen Report.
Meanwhile the ingredient that the malicious insect vector medicament of Citrus Huanglongbing pathogen biography is eliminated in the present invention is as follows: clothianidin 10%, thiazine White carbon black 3%, silicon is added using clothianidin, Buprofezin and flonicamid as effective component in ketone 20%, flonicamid 10% Diatomaceous earth 2%, lauryl sodium sulfate 2%, pull open powder 2%, maleic acid di-sec-octyl 2%, remaining is deionized water, through sand Grinding machine attrition process is at the second Thief zone type insecticidal suspending agent composition.And according to the retrieval of inventor, the text of the prior art It there are no in offering and use clothianidin, Buprofezin and flonicamid for effective component to prepare Thief zone suspending agent and for preventing and treating Citrus Huanglongbing pathogen passes the report of malicious insect vector wood louse.
The present embodiment is created the systematic treating method of Citrus Huanglongbing pathogen by 3 technical combinations, is applicable not only to treatment mandarin orange Tangerine yellow twig, the plant for being also suitable for occurring for sweet orange, lemon and shaddock class yellow twig carry out symptomatic treatment, restore disease plant Healthy growth, the scope of application is wider, and application value is big, and economic and social benefit is very significant.
It should be understood by those ordinary skilled in the art that: the discussion of any of the above embodiment is exemplary only, not It is intended to imply that the scope of the present disclosure (including claim) is limited to these examples；Under thinking of the invention, above embodiments Or it can also be combined between the technical characteristic in different embodiments, and there is being permitted for different aspect of the invention as above Mostly other variations, for simplicity, they are not provided in details.Therefore, all within the spirits and principles of the present invention, done Any omission, modification, equivalent replacement, improvement etc., should all be included in the protection scope of the present invention.

Claims (8)

1. a kind of systematic treating method of Citrus Huanglongbing pathogen, which is characterized in that in citrus orchard over-all application mandarin tree dedicated fertilizer, The aqueous suspensions symptomatic treatment of the first bactericidal suspending agent of Thief zone type is continuously sprayed for disease plant simultaneously, is sprayed 1 time within 7-10 days, Continuous processing 3-4 times kills pathogenetic bacteria；When mandarin tree wood louse occurs, suspend to the second Thief zone type desinsection of full tree sprinkling The aqueous suspensions of agent are eliminated and pass malicious insect vector wood louse；
Wherein the first bactericidal suspending agent of Thief zone type includes antibacterial peptide, chlorogenic acid and the gloomy copper of thiophene, and the second Thief zone type desinsection is outstanding Floating agent includes clothianidin, Buprofezin and flonicamid.
2. the systematic treating method of Citrus Huanglongbing pathogen according to claim 1, which is characterized in that the mandarin tree special fertilizer Material includes function bacterium and mixed mineral particulate vector；The function bacterium is adsorbed in the mixed mineral particulate vector, the function It can bacterium bag nitrogen-fixing bacteria containing ultra high efficiency, phosphate solubilizing bacteria and potassium solubilizing bacteria；
The ultra high efficiency nitrogen-fixing bacteria, are named as SDTB-0035, and classification naming is bacillus amyloliquefaciens (Bacillus Amyloliquefaciens), it is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, preservation address It is Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, the deposit date is on April 26th, 2019, deposit number CGMCC NO.17635；
The phosphate solubilizing bacteria, is named as SDTB-0043, and classification naming is bacillus amyloliquefaciens (Bacillus Amyloliquefaciens), it is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, preservation address It is Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, the deposit date is on April 26th, 2019, deposit number CGMCC NO.17637；
The potassium solubilizing bacteria, is named as SDTB-0052, and classification naming is bacillus amyloliquefaciens (Bacillus Amyloliquefaciens), it is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, preservation address It is Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, the deposit date is on April 26th, 2019, deposit number CGMCC NO.17639；
The mixed mineral particulate vector includes each component of following parts by weight: 20-40 parts of humic acid, 5-15 parts of carbonatoapatites, 5-15 parts of niters, 3-5 parts of shells, 5-15 parts of magnesium silicates, 5-15 parts of perlites, 5-20 parts of clays and 0.5-2 parts of trapping agent hydroxyls Propyl methocel.
3. the systematic treating method of Citrus Huanglongbing pathogen according to claim 2, which is characterized in that the mixed mineral particle The preparation method of carrier includes the following steps:
(a) by 20-40 parts of humic acid, 5-15 parts of carbonatoapatites, 5-15 parts of niters, 3-5 parts of shells, 5-15 parts of magnesium silicates, 5- 15 parts of perlites and 5-20 parts of clays are crushed to diameter 3.5-5mm, form natural mixed mineral powder；
(b) 0.5-2 parts of hydroxypropyl methyl celluloses are configured to the hydroxypropyl methyl cellulose aqueous solution that mass concentration is 5%；
In natural mixed mineral powder input mixer, 5% hydroxypropyl methyl cellulose aqueous solution is sprayed under stiring, by mineral Powder body material capture；
(c) the mineral powder body material after capturing hydroxypropyl methyl cellulose is transported to pelletizer granulation, and by mixed mineral For grain in 150-200 DEG C of dryness finalization, the mixed mineral containing various nutrient elements and porous structure is made in quenching to room temperature Grain carrier.
4. the systematic treating method of Citrus Huanglongbing pathogen according to claim 2, which is characterized in that the mandarin tree special fertilizer Material is prepared using following methods:
(1) fine using humic acid, carbonatoapatite, niter, shell, magnesium silicate, perlite, clay and trapping agent hydroxypropyl methyl Dimension element is raw material, prepares mixed mineral particulate vector；
(2) ultra high efficiency nitrogen-fixing bacteria, phosphate solubilizing bacteria and potassium solubilizing bacteria are inoculated in first cell culture medium respectively to cultivate, obtain level-one bacterium Kind；
First class inoculum is inoculated in secondary medium respectively to cultivate, obtains second class inoculum；
Second class inoculum is inoculated in fermentation medium respectively to cultivate, obtains bacterium containing high-efficiency nitrogen-fixing, phosphate solubilizing bacteria and potassium solubilizing bacteria Fermentation liquid；
(3) fermentation liquid of ultra high efficiency nitrogen-fixing bacteria, phosphate solubilizing bacteria and potassium solubilizing bacteria is uniformly mixed, mixed mineral particulate vector is sprayed Mist is adsorbed in ultra high efficiency nitrogen-fixing bacteria thallus, phosphate solubilizing bacteria thallus and potassium solubilizing bacteria thallus in mixed mineral particulate vector, obtains citrus Set dedicated fertilizer.
5. the systematic treating method of Citrus Huanglongbing pathogen according to claim 1, which is characterized in that the first Thief zone type The ingredient of bactericidal suspending agent is as follows: antibacterial peptide 1~5%, chlorogenic acid 1~5%, the gloomy copper 10~40% of thiophene, white carbon black 1~5%, Diatomite 1~5%, lauryl sodium sulfate 1~3%, pull open powder 1~3%, sodium lignin sulfonate 1~5% and maleic acid Di-sec-octyl 1~3%, remaining is deionized water.
6. the systematic treating method of Citrus Huanglongbing pathogen according to claim 4, which is characterized in that by antibacterial peptide 1~5%, Chlorogenic acid 1~5%, the gloomy copper 10~40% of thiophene, white carbon black 1~5%, diatomite 1~5%, lauryl sodium sulfate 1~3% are drawn Powder 1~3% is opened, sodium lignin sulfonate 1~5% and maleic acid di-sec-octyl 1~3% are dissolved in deionized water, through being sanded Machine attrition process is at the first bactericidal suspending agent of Thief zone type；
The first bactericidal suspending agent of Thief zone type is diluted with water 500-750 times and is made into aqueous suspensions, spraying to use.
7. the systematic treating method of Citrus Huanglongbing pathogen according to claim 1, which is characterized in that the second Thief zone type desinsection The ingredient of suspending agent is as follows: clothianidin 5~20%, Buprofezin 10~30%, flonicamid 5~20%, white carbon black 1~5%, Diatomite 1~5%, lauryl sodium sulfate 1~3%, pull open powder 1~3%, maleic acid di-sec-octyl 1~3%, remaining For deionized water.
8. the systematic treating method of Citrus Huanglongbing pathogen according to claim 7, which is characterized in that by clothianidin 5~20%, Buprofezin 10~30%, flonicamid 5~20%, white carbon black 1~5%, diatomite 1~5%, lauryl sodium sulfate 1~ 3%, pull open powder 1~3% and maleic acid di-sec-octyl 1~3% are dissolved in deionized water, through sand mill attrition process at Two Thief zone type insecticidal suspending agents；
Second Thief zone type insecticidal suspending agent is diluted with water 1000-3000 times and is made into aqueous suspensions, spraying to use.
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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NL2032080B1 (en) * 2022-06-05 2023-03-20 Shandong Huitaihua Biotechnology Co Ltd Natural bio-organic fertilizer for providing full nutrition elements for plants and application thereof
CN117770253A (en) * 2023-12-18 2024-03-29 华中农业大学 Application of chlorogenic acid and chlorogenic acid-rich substance in prevention and control of citrus yellow dragon disease

Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1411724A (en) * 2002-10-09 2003-04-23 华南农业大学 Nimbin and thiazinedone mixed pesticide preparation
CN101475420A (en) * 2009-01-23 2009-07-08 深圳市朗钛生物科技有限公司 Fertilizer water-dispersion granule and preparation thereof
CN101642097A (en) * 2009-09-07 2010-02-10 山东农业大学 Clothianidin sustained and controlled release granule composite
CN102992827A (en) * 2012-12-14 2013-03-27 山东新超农业科技有限公司 Active organic bacterial fertilizer and preparation method thereof
CN104222075A (en) * 2014-08-29 2014-12-24 上海大井生物工程有限公司 Temperature-sensitive trichoderma harzianum microbial agent carrier formula and preparation process thereof
CN104823626A (en) * 2015-03-31 2015-08-12 杨正庭 Agricultural ecological technique capable of preventing and controlling liberobacter asiaticum as well as harvesting organic oranges
CN106316543A (en) * 2015-06-15 2017-01-11 张玉琴 Special active organic microbial fertilizer for Sophora flavescens, and making method thereof
CN108484270A (en) * 2018-05-16 2018-09-04 陕西科技大学 A kind of antibacterial peptide bio-feritlizer and preparation method thereof
CN108925333A (en) * 2018-05-25 2018-12-04 杭州鲜直达农业科技有限公司 A kind of ecological preventing control method of Citrus Huanglongbing pathogen
CN109699658A (en) * 2017-10-26 2019-05-03 三江侗族自治县中吉牛势测土配肥中心 One kind containing insecticide and fungicide sustained-release granular formulation

Patent Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1411724A (en) * 2002-10-09 2003-04-23 华南农业大学 Nimbin and thiazinedone mixed pesticide preparation
CN101475420A (en) * 2009-01-23 2009-07-08 深圳市朗钛生物科技有限公司 Fertilizer water-dispersion granule and preparation thereof
CN101642097A (en) * 2009-09-07 2010-02-10 山东农业大学 Clothianidin sustained and controlled release granule composite
CN102992827A (en) * 2012-12-14 2013-03-27 山东新超农业科技有限公司 Active organic bacterial fertilizer and preparation method thereof
CN104222075A (en) * 2014-08-29 2014-12-24 上海大井生物工程有限公司 Temperature-sensitive trichoderma harzianum microbial agent carrier formula and preparation process thereof
CN104823626A (en) * 2015-03-31 2015-08-12 杨正庭 Agricultural ecological technique capable of preventing and controlling liberobacter asiaticum as well as harvesting organic oranges
CN106316543A (en) * 2015-06-15 2017-01-11 张玉琴 Special active organic microbial fertilizer for Sophora flavescens, and making method thereof
CN109699658A (en) * 2017-10-26 2019-05-03 三江侗族自治县中吉牛势测土配肥中心 One kind containing insecticide and fungicide sustained-release granular formulation
CN108484270A (en) * 2018-05-16 2018-09-04 陕西科技大学 A kind of antibacterial peptide bio-feritlizer and preparation method thereof
CN108925333A (en) * 2018-05-25 2018-12-04 杭州鲜直达农业科技有限公司 A kind of ecological preventing control method of Citrus Huanglongbing pathogen

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
刘新: "《农药安全使用技术》", 30 September 2014, 福建科学技术出版社 *
呙于明: "《家禽营养》", 30 April 2016, 中国农业大学出版社 *
李毅，洪华珠，陈振民: "《生物农药 第2版》", 30 April 2017, 华中师范大学出版社 *
王开运: "超高效固氮菌和生物有机肥的创新与应用前景", 《农业科技通讯》 *
王筠默: "《中药药理学》", 30 April 1985 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NL2032080B1 (en) * 2022-06-05 2023-03-20 Shandong Huitaihua Biotechnology Co Ltd Natural bio-organic fertilizer for providing full nutrition elements for plants and application thereof
CN117770253A (en) * 2023-12-18 2024-03-29 华中农业大学 Application of chlorogenic acid and chlorogenic acid-rich substance in prevention and control of citrus yellow dragon disease
CN117770253B (en) * 2023-12-18 2024-07-02 华中农业大学 Application of chlorogenic acid and chlorogenic acid-rich substance in prevention and control of citrus yellow dragon disease

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