CN103159570A - Solid microbial fertilizer and preparation method thereof - Google Patents
Solid microbial fertilizer and preparation method thereof Download PDFInfo
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- CN103159570A CN103159570A CN2011103035070A CN201110303507A CN103159570A CN 103159570 A CN103159570 A CN 103159570A CN 2011103035070 A CN2011103035070 A CN 2011103035070A CN 201110303507 A CN201110303507 A CN 201110303507A CN 103159570 A CN103159570 A CN 103159570A
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- 239000003337 fertilizer Substances 0.000 title claims abstract description 32
- 239000007787 solid Substances 0.000 title claims abstract description 29
- 238000002360 preparation method Methods 0.000 title claims abstract description 14
- 230000000813 microbial effect Effects 0.000 title abstract 2
- 230000001580 bacterial effect Effects 0.000 claims abstract description 18
- 241000894006 Bacteria Species 0.000 claims abstract description 14
- 241000193830 Bacillus <bacterium> Species 0.000 claims abstract description 13
- 241000193744 Bacillus amyloliquefaciens Species 0.000 claims abstract description 8
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 5
- 240000008042 Zea mays Species 0.000 claims abstract description 4
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims abstract description 4
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims abstract description 4
- 235000005822 corn Nutrition 0.000 claims abstract description 4
- 238000000855 fermentation Methods 0.000 claims abstract description 4
- 230000004151 fermentation Effects 0.000 claims abstract description 4
- 210000003608 fece Anatomy 0.000 claims description 19
- 239000010871 livestock manure Substances 0.000 claims description 19
- 239000007788 liquid Substances 0.000 claims description 18
- 235000015097 nutrients Nutrition 0.000 claims description 9
- 238000009423 ventilation Methods 0.000 claims description 5
- 239000002054 inoculum Substances 0.000 claims description 4
- 239000000725 suspension Substances 0.000 claims description 4
- 238000010298 pulverizing process Methods 0.000 claims description 3
- 230000001954 sterilising effect Effects 0.000 claims description 3
- 238000012546 transfer Methods 0.000 claims description 3
- 239000002028 Biomass Substances 0.000 claims description 2
- 238000004659 sterilization and disinfection Methods 0.000 claims description 2
- 238000001035 drying Methods 0.000 claims 2
- 240000002024 Gossypium herbaceum Species 0.000 claims 1
- 235000004341 Gossypium herbaceum Nutrition 0.000 claims 1
- 239000002689 soil Substances 0.000 abstract description 14
- 230000000694 effects Effects 0.000 abstract description 8
- 238000004519 manufacturing process Methods 0.000 abstract description 5
- 239000000126 substance Substances 0.000 abstract description 4
- 230000003213 activating effect Effects 0.000 abstract description 2
- 201000010099 disease Diseases 0.000 abstract description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 2
- 235000014469 Bacillus subtilis Nutrition 0.000 abstract 3
- 239000001963 growth medium Substances 0.000 abstract 3
- 238000012258 culturing Methods 0.000 abstract 1
- 230000035699 permeability Effects 0.000 abstract 1
- 230000002265 prevention Effects 0.000 abstract 1
- 230000001737 promoting effect Effects 0.000 abstract 1
- 238000011218 seed culture Methods 0.000 abstract 1
- 239000002881 soil fertilizer Substances 0.000 abstract 1
- 238000003860 storage Methods 0.000 abstract 1
- 241000196324 Embryophyta Species 0.000 description 6
- 229920001817 Agar Polymers 0.000 description 4
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
- 239000008272 agar Substances 0.000 description 4
- 229920000742 Cotton Polymers 0.000 description 3
- 244000299507 Gossypium hirsutum Species 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 230000035558 fertility Effects 0.000 description 3
- 244000005700 microbiome Species 0.000 description 3
- 239000002893 slag Substances 0.000 description 3
- 239000001888 Peptone Substances 0.000 description 2
- 108010080698 Peptones Proteins 0.000 description 2
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 2
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 235000015278 beef Nutrition 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 239000000284 extract Substances 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- 235000019319 peptone Nutrition 0.000 description 2
- 229910052698 phosphorus Inorganic materials 0.000 description 2
- 239000011574 phosphorus Substances 0.000 description 2
- 239000011591 potassium Substances 0.000 description 2
- 229910052700 potassium Inorganic materials 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 241000220317 Rosa Species 0.000 description 1
- 241001655322 Streptomycetales Species 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 239000003905 agrochemical Substances 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 229940041514 candida albicans extract Drugs 0.000 description 1
- 238000005056 compaction Methods 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 238000009313 farming Methods 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 239000003673 groundwater Substances 0.000 description 1
- 230000008595 infiltration Effects 0.000 description 1
- 238000001764 infiltration Methods 0.000 description 1
- 238000002386 leaching Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 238000009335 monocropping Methods 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
- 230000021715 photosynthesis, light harvesting Effects 0.000 description 1
- 244000144977 poultry Species 0.000 description 1
- 229930000044 secondary metabolite Natural products 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
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Abstract
The invention provides a solid biological bacterial fertilizer, wherein the biological bacteria are bacillus, and the viable count of the bacillus is more than or equal to 10 hundred million/g. The bacillus comprises bacillus amyloliquefaciens and bacillus subtilis. The fertilizer is free of substances harmful to the environment, has good fertilizer efficiency and strong stress resistance, and has a comprehensive effect of improving the soil micro-ecological structure. The preparation method comprises the following steps: respectively culturing bacillus amyloliquefaciens and bacillus subtilis on a BPY solid culture medium and a NA solid culture medium, then inoculating the bacillus amyloliquefaciens and the bacillus subtilis into a seed culture medium, fermenting to obtain seed bacteria fermentation liquor, crushing corncobs into 3-6mm, then uniformly mixing the corn cobs with the two seed bacteria fermentation liquors under an aseptic condition according to the proportion of 1: 1.5-2, and continuously fermenting to obtain the microbial fertilizer. The preparation process is simple, the cost is low, the storage is easy, and the automatic production is easy to realize. The invention has the functions of activating soil, increasing soil fertilizer efficiency and air permeability, promoting the growth of crops and improving the yield of the crops. And has certain prevention and treatment effect on soil-borne diseases of various crops.
Description
Technical field
The present invention relates to a kind of bacterial manure of agricultural technology field, particularly a kind of solid microbe bacterial manure that is suitable for multiple kinds of crops and preparation method thereof.
Background technology
In recent years, along with rural urbanization and the specialized development of livestock-raising, the use of person poultry muck in the farmland is fewer and feweri, and inorganic fertilizer becomes to take as the leading factor as the use that improves crop yield.For many years, the peasant only payes attention to the use of nitrogen, phosphorus, potassium, the pattern of farming of each department is single in addition, continuous cropping year after year, a large amount of harmful secondary metabolites of crop secretion increase year by year, cause the soil microorganisms profitable strain fewer and feweri, cause soil fertility to descend, soil compaction, nutrient imbalance, resistance appears in crop.Conventional chemical fertilizer utilization ratio is very low, and the utilization ratio of nitrogen is about 30%-35%, and the utilization ratio of phosphorus is about 10%-20%, and the utilization ratio of potassium is about 30%-35%.A large amount of chemical fertilizer enters into river and groundwater resource by effects such as infiltration and leachings, causes a large amount of wastes and the environmental pollution of the energy.Therefore, be necessary to increase the use of some beneficial microorganism fertilizer, improving the certain effects of aspect performance such as farmland micro-ecological environment, environmental contamination reduction and energy dissipation.
Yet, the at present production of microbial-bacterial fertilizer and use and also have this some difficult problems, 1. manufacture with the wrapping process condition strict, carrying and preserve and need good condition, so production cost is high and easy pollution, often bacterial manure becomes waste product in batch; 2. the shelf life of finished product is short; 3. after the bacterial manure microorganism enters soil, be not easy to form dominant colony, bacterial manure effect validity period is short and effect is unstable; 4. also need avoid the restriction of the agricultural measures such as agricultural chemicals, chemical fertilizer and environmental factor when use in the field.
Summary of the invention
The purpose of this invention is to provide and do not contain environmentally harmful material, have fertilizer efficiency good, strong stress resistance has a kind of solid microbe bacterial manure of the net effect of improvement soil little ecologic structure.
Another object of the present invention provides a kind of preparation technology simple, and cost is low, easily preserves, and easily is automated the preparation method of the solid microbe bacterial manure of production.
Another object of the present invention provides a kind of solid microbe bacterial manure applicable to activating soil, increases soil fertility and ventilation property, promotes the growth of farm crop and improves crop yield.
The present invention has a purpose to provide a kind of solid microbe bacterial manure to bring into play certain effect to the control of multiple kinds of crops soil-borne disease.
The invention provides a kind of solids bio-bacterial manure, biomass is genus bacillus, described bacillus living number 〉=1,000,000,000/gram.Described genus bacillus comprises bacillus amyloliquefaciens and subtilis.
The present invention also provides a kind of preparation method of solid microbe fertilizer, concrete steps:
Step 1 is got the bacillus amyloliquefaciens after purifying, and it is inoculated on the BPY solid medium, cultivates, and after 48 hours, is linked in the BPY liquid nutrient medium, on shaking table, continues to cultivate 48 hours, obtains the seed fermented liquid.
Described BPY solid medium is: beef extract 0.5%, and peptone 1%, yeast extract 0.5%, NaCl 0.5%, glucose 0.5%, agar 1%-2% adds water to 1000mL, pH value=7.
Described BPY liquid nutrient medium is the above-mentioned substratum that agar is mixed with that do not add.
Step 2 is got the subtilis after purifying, and it is inoculated on the NA solid medium, cultivates, and after 48 hours, is linked on the NA liquid nutrient medium, continues to cultivate 48 hours on shaking table, obtains the seed fermented liquid.
Described NA solid medium is: beef extract 0.5%-0.6%, peptone 1%, sucrose 2%.Agar 1.4%-2% adds water to 1000mL, pH value=7.
Described NA liquid nutrient medium is the above-mentioned substratum that does not make agar be mixed with.
Step 3, after pulverizing through pulverizer, and sterilization corn cob and two all daughter bacteria fermented liquids of 3 hours in 121 ℃ of Autoclaves, according to 1: 1.5-2: the ratio of 1.5-2 is evenly mixed under aseptic condition, and transfers in biochemical cultivation case and further cultivate.30 ± 1 ℃, cultivated 48 hours, then improve temperature to 35 ± 1 ℃, continue to cultivate 24 hours.
Step 4 after cultivation is completed, is taken out, and is placed in dry ventilation under 35 ± 1 ℃ of air dry ovens or natural condition.Dry 6-7 days, humidity≤10%.Can obtain this solid microbe bacterial manure.
Accompanying drawing 1 solid microbe bacterial manure of the present invention.
Embodiment:
Step 1 is got the bacillus amyloliquefaciens after purifying, and it is inoculated on the BPY solid medium, cultivates, and after 48 hours, making genus bacillus content is 10
9The bacteria suspension of individual/ML, the inoculum size according to 5% are linked in the BPY liquid nutrient medium, on the 200r/min shaking table, and 30 ± 1 ℃, cultivated 48 hours, obtain the seed fermented liquid.
Step 2 is got the subtilis after purifying, and it is inoculated on the NA solid medium, cultivates, and after 48 hours, making genus bacillus content is 10
9The bacteria suspension of individual/ML, the inoculum size according to 5% are linked on the NA liquid nutrient medium, at 200r/min, and on shaking table 28 ± 1 ℃, cultivated 48 hours, obtain the seed fermented liquid,
Step 3, after pulverizing through pulverizer, and corn cob and two all daughter bacteria fermented liquids of sterilizing 3 hours in 121 ℃ of Autoclaves, according to 1: 1.5-2: 1.5-2 evenly mixes than under row aseptic condition.Transfer in biochemical cultivation case and further cultivate, 30 ± 1 ℃, cultivated 48 hours, then improve temperature to 35 ± 1 ℃, continue to cultivate 24 hours.
Step 4 after cultivation is completed, is taken out the dry ventilation in the air dry oven of 35 ℃ or under natural condition with the solid fermentation thing, dry 6-7 days, humidity≤10%.Can obtain this solid microbe bacterial manure.The granular size of microbial-bacterial fertilizer is 3～6mm.
In order to understand better essence of the present invention, the below provides this solid microbe bacterial manure indoor, the short of cotton seedling to be given birth to test, and compares with patent bacterial manure " rose yellow streptomycete mutation 0116 fermented liquid bacterium slag as fertilizer sources ".
Result is as follows:
The impact of table 1 microbial-bacterial fertilizer on the crops seedling stage fertility
As can be seen from Table 1,
Microbial-bacterial fertilizer: the cotton seedling of soil=processing in 1: 200, average root is long is 15.37cm, several 32 of lateral root, seedling stage, the stem height was 39.63cm, individual plant is heavily 9.97g.
Bacterium slag fertilizer: the cotton seedling of soil=processing in 1: 200, average root is long is 13.43cm, several 28 of lateral root, seedling stage, the stem height was 31.33cm, individual plant is heavily 7.7g.
In the situation of not applying fertilizer, average root is long is 9.63cm, several 25.67 of lateral root, and seedling stage, the stem height was 27.5cm, individual plant is heavily 6.1g.
Significance analysis:
Microbial-bacterial fertilizer: soil=with do not apply fertilizer compare at 1: 200, root length is remarkable at 5% level difference, and is remarkable at 1% level error heteropole.The lateral root number is not remarkable at 5% level difference, and the level difference 1% is not remarkable.The high level difference 5% of stem is remarkable, and is remarkable at 1% level error heteropole.It is remarkable that individual plant focuses on 5% level difference, remarkable at 1% level error heteropole.
Microbial-bacterial fertilizer: soil=with do not apply fertilizer compare at 1: 200, root length is remarkable at 5% level difference, and is remarkable at 1% level error heteropole.The lateral root number is not remarkable at 5% level difference, and is remarkable at 1% level error heteropole.The high level difference 5% of stem is remarkable, and is remarkable at 1% level error heteropole.It is remarkable that individual plant focuses on 5% level difference, remarkable at 1% level error heteropole.
Bacterium slag fertilizer: soil=with do not apply fertilizer compare at 1: 200, root length is remarkable at 5% level difference, and is remarkable at 1% level error heteropole.The lateral root number is not remarkable at 5% level difference, and the level difference 1% is not remarkable.The high level difference 5% of stem is not remarkable, and is remarkable at 1% level error heteropole.It is not remarkable that individual plant focuses on 5% level difference, remarkable at 1% level error heteropole.
Claims (8)
1. a solid microbe bacterial manure, is characterized in that, biomass is genus bacillus, and the viable count of affiliated genus bacillus is 〉=10 hundred million/gram.
2. a kind of bio-bacterial manure as claimed in claim 1, is characterized in that, described genus bacillus comprises bacillus amyloliquefaciens and subtilis, all comes from the body of healthy cotton plant, belongs to endogenetic bacteria.
3. the preparation method of a solid microbe fertilizer, is characterized in that, comprises the steps:
Step 1 is got bacillus amyloliquefaciens, and it is inoculated on the BPY solid medium, inserts in 30 ± 1 ℃ of growth cabinets and cultivates, and after 48 hours, making genus bacillus content is 10
9The bacteria suspension of individual/mL, the inoculum size according to 5% are linked in the BPY liquid nutrient medium, on shaking table, continue to cultivate 48 hours, obtain the seed fermented liquid.
Step 2 is got subtilis, and it is inoculated on the NA solid medium, inserts in 30 ± 1 ℃ of growth cabinets and cultivates, and after 48 hours, making genus bacillus content is 10
9The bacteria suspension of individual/mL, the inoculum size according to 5% are linked on the NA liquid nutrient medium, continue to cultivate 48 hours on shaking table, obtain the seed fermented liquid,
Step 3, after pulverizing through pulverizer, and sterilization corn cob and two all daughter bacteria fermented liquids of 3 hours in 121 ℃ of Autoclaves, according to 1: 1.5-2: 1.5-2 evenly mixes than under row aseptic condition, transfers in biochemical cultivation case and further cultivates.
Step 4 after cultivation is completed, is taken out, and is placed in dry ventilation under air dry oven or natural condition, drying.Can obtain this solid microbe bacterial manure.
4. according to the preparation method of claims 3 described microbial-bacterial fertilizers, it is characterized in that, the culture condition of step 1 is 30 ± 1 ℃, and 200r/min cultivated 48 hours.
5. according to the preparation method of claims 3 described microbial-bacterial fertilizers, it is characterized in that, the culture condition of step 2 is 28 ± 1 ℃, and 200r/min cultivated 48 hours.
6. according to the preparation method of claims 3 described microbial-bacterial fertilizers, it is characterized in that, the culture condition of step 3 is 30 ± 1 ℃, cultivates 48 hours, then improves temperature to 35 ± 1 ℃, cultivates 24 hours.
7. according to the preparation method of claims 3 described microbial-bacterial fertilizers, it is characterized in that, in step 4, described drying, in the air dry oven of 35 ℃ or under natural condition, dry dry ventilation with the solid fermentation thing, and dry 6-7 days, humidity≤10%.
8. according to the preparation method of claims 3 described microbial-bacterial fertilizers, it is characterized in that, the granular size of described microbial-bacterial fertilizer is 3～6mm.
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| CN2011103035070A CN103159570A (en) | 2011-12-12 | 2011-12-12 | Solid microbial fertilizer and preparation method thereof |
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| CN2011103035070A CN103159570A (en) | 2011-12-12 | 2011-12-12 | Solid microbial fertilizer and preparation method thereof |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105016839A (en) * | 2014-04-18 | 2015-11-04 | 河北农业大学 | Nutritional biological tablet |
| CN105272407A (en) * | 2015-11-17 | 2016-01-27 | 山东佐田氏生物科技有限公司 | Method and application for preparing biological polypeptide organic fertilizer through microbial fermentation |
| CN106119143A (en) * | 2016-03-16 | 2016-11-16 | 河北润雨肥料科技有限公司 | A kind of disease resistance growth promotion microbial-bacterial fertilizer and preparation method thereof |
| CN107163949A (en) * | 2017-06-07 | 2017-09-15 | 刘铜 | Soil conditioner Trichoderma atroviride DQ1 compound powders TLB is prepared and applied |
| CN107711891A (en) * | 2017-10-12 | 2018-02-23 | 天津坤禾生物科技股份有限公司 | A kind of method prevented and treated matrimony vine root rot and extremely set |
| CN108118000A (en) * | 2017-11-29 | 2018-06-05 | 山东神农氏生物技术有限公司 | A kind of high concentration disease-resistant compound microbial bacterial manure and production method |
| CN108863550A (en) * | 2018-07-25 | 2018-11-23 | 龙岩市农业科学研究所 | A kind of dedicated disease prevention growth-promoting composite microbic bacterial fertilizer of tea tree and preparation method thereof |
| CN112409100A (en) * | 2020-11-19 | 2021-02-26 | 广东唯新生物科技有限公司 | Microbial organic functional fertilizer for preventing and treating ginger soil-borne diseases and preparation method thereof |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105016839A (en) * | 2014-04-18 | 2015-11-04 | 河北农业大学 | Nutritional biological tablet |
| CN105272407A (en) * | 2015-11-17 | 2016-01-27 | 山东佐田氏生物科技有限公司 | Method and application for preparing biological polypeptide organic fertilizer through microbial fermentation |
| CN106119143A (en) * | 2016-03-16 | 2016-11-16 | 河北润雨肥料科技有限公司 | A kind of disease resistance growth promotion microbial-bacterial fertilizer and preparation method thereof |
| CN107163949A (en) * | 2017-06-07 | 2017-09-15 | 刘铜 | Soil conditioner Trichoderma atroviride DQ1 compound powders TLB is prepared and applied |
| CN107711891A (en) * | 2017-10-12 | 2018-02-23 | 天津坤禾生物科技股份有限公司 | A kind of method prevented and treated matrimony vine root rot and extremely set |
| CN107711891B (en) * | 2017-10-12 | 2018-08-14 | 天津坤禾生物科技集团股份有限公司 | A method of prevention matrimony vine root rot is extremely set |
| CN108118000A (en) * | 2017-11-29 | 2018-06-05 | 山东神农氏生物技术有限公司 | A kind of high concentration disease-resistant compound microbial bacterial manure and production method |
| CN108863550A (en) * | 2018-07-25 | 2018-11-23 | 龙岩市农业科学研究所 | A kind of dedicated disease prevention growth-promoting composite microbic bacterial fertilizer of tea tree and preparation method thereof |
| CN112409100A (en) * | 2020-11-19 | 2021-02-26 | 广东唯新生物科技有限公司 | Microbial organic functional fertilizer for preventing and treating ginger soil-borne diseases and preparation method thereof |
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