CN103964946B - A kind of paddy rice Special compound microbial fertilizer and preparation method thereof - Google Patents

A kind of paddy rice Special compound microbial fertilizer and preparation method thereof Download PDF

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CN103964946B
CN103964946B CN201410158319.7A CN201410158319A CN103964946B CN 103964946 B CN103964946 B CN 103964946B CN 201410158319 A CN201410158319 A CN 201410158319A CN 103964946 B CN103964946 B CN 103964946B
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fertilizer
component
powder
composite
complex microorganism
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CN103964946A (en
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丰来
罗志威
粟静
郭帅
吕黎
王蕾
周艳
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湖南泰谷生物科技股份有限公司
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Abstract

The invention provides a kind of paddy rice Special compound microbial fertilizer, this fertilizer consists of the following composition: fertilizer component, inorganic composite fertilizer component, wilkinite and complex microorganism component; Contain in described composite microbiological fertilizer: nitrogen 10-15%, phosphorus 4-8%, potassium 6-10%, organic matter 25%, complex microorganism number content 2-18 hundred million cfu/g.Paddy rice Special compound microbial fertilizer of the present invention, can meet the full nutrition needed for paddy growth, improves rice quality, realizes increasing both production and income, can improve soil fertility of paddy field simultaneously, improve paddy soil environment, effectively prevent and treat the generation of rice disease.

Description

A kind of paddy rice Special compound microbial fertilizer and preparation method thereof

Technical field

The present invention relates to agricultural fertilizer field, specifically, relate to a kind of paddy rice Special compound microbial fertilizer and preparation method thereof.

Background technology

China owing to using chemical fertilizer in a large number for a long time, causes soil compaction at present, and soil fertility declines, and underground water is contaminated, and biological activity declines, and soil organic matter content is extremely low, and agricultural product quality declines.For addressing this problem, there is all kinds of fertilizer in the market, as compound organic and inorganic fertilizer, compoiste fertilizer, microorganism fertilizer.Fertilizer fertilizer efficiency is longer, can increase and upgrade the soil organism, improves soil physico-chemical property and biological activity.Microorganism fertilizer can improve beneficial microorganism quantity in soil, strengthens crop to the absorption of soil moisture and nutrient and utilization.But these fertilizer can not fundamentally solve the soil compaction caused in fertilising process, the problem that soil fertility declines, can't fundamentally substituted chemistry fertilizer.So organic and inorganic and microorganism fertilizer combines become the new direction that a kind of Dasanyuan fertilizer becomes Chinese fertilizer.

The present Rice Cropping range of distribution of China is based on south, account for 94% of national rice growing area, Hunan have " land of plenty " and the good reputation of " province of the Yuan Dynasty is ripe, all over the world foot ", rice yield is the first in the nation position, produces the grain accounting for the whole nation 6% with the arable land accounting for the whole nation 3%.But due to for a long time, too focus on output in eating rice production, unreasonable use chemical fertilizer, chemical pesticide, cause larger impact to existing Rice Cropping pattern, soil fertility declines, and the quality safety of rice products also once received much concern.

At present, have a lot of fertilizer and microorganism fertilizer to be applied to paddy rice, fertilizer and microorganism fertilizer effectively can improve edatope, promote soil fertility, but the nutritive ingredient of simple fertilizer and microorganism fertilizer is not very comprehensive, also need a large amount of inorganic fertilizers come with the use of.Also there are now many companies at research organic and inorganic bio-fertilizer, if China Patent Publication No. is the patent of invention of CN102701884A, disclose a kind of bio-compound fertilizer containing organic and inorganic components and method for making thereof, this product nutritive ingredient relatively comprehensively fully, but the loss of microorganism is larger in actual production process, and not easily preserve, effective bacterial content is too low, the problem such as play a role limited.This method is by microorganism granulation, and adopt granulation post-drying means to process product, in actual production, bake out temperature more than 50 DEG C, time length longer, ambient moisture larger time, microorganism has larger loss, if lower than 50 DEG C, time of drying is longer, production capacity is very low, and production cost is higher.

Summary of the invention

In order to solve problems of the prior art, the object of this invention is to provide a kind of Novel rice Special compound microbial fertilizer.

Technical scheme of the present invention is as follows:

First the present invention provides a kind of paddy rice Special compound microbial fertilizer, consists of the following composition: fertilizer component, inorganic composite fertilizer component, wilkinite and complex microorganism component; Contain in described composite microbiological fertilizer: nitrogen 10-15%, phosphorus 4-8%, potassium 6-10%, organic matter 25%, complex microorganism number content 2-18 hundred million cfu/g.

Wherein, described fertilizer component is formed by dregs of beans, rapeseed meal, flue-cured tobacco straw powder compost fermentation; Wherein the mass ratio of dregs of beans, rapeseed meal, flue-cured tobacco straw powder is 6: 3: 1.

Wherein, described inorganic composite fertilizer component is made up of urea, potassium sulfate and calcium superphosphate.

Further, described inorganic composite fertilizer component is made up of 46% urea, 45% potassium sulfate and 53% calcium superphosphate; Wherein the mass ratio of 46% urea, 45% potassium sulfate and 53% calcium superphosphate is 18.8 ~ 29.7: 8.1 ~ 17: 8.2 ~ 15.7.

Wherein, described complex microorganism component is made up of subtilis, bacillus megaterium, trichoderma harziarum and Streptomyces hygrospinosus four kinds of microorganisms; The living bacteria count ratio of subtilis in described complex microorganism component, bacillus megaterium, trichoderma harziarum and Streptomyces hygrospinosus is 4: 2: 1: 1.

Present invention also offers the preparation method of aforementioned composite microbiological fertilizer, said method comprising the steps of:

1) fertilizer component, inorganic composite fertilizer component, wilkinite are mixed in the ratio of 33.3:35.1 ~ 62.4:0.3 ~ 27.6, be a granulated into the particle of particle diameter 4mm, control moisture about 12%, obtain the composite organic-inorganic fertilizer work in-process of nitrogenous 10-15%, phosphorus 4-8%, potassium 6-10%, organic matter 25%;

2) composite organic-inorganic fertilizer work in-process step 1) obtained and complex microorganism component mix for the ratio of 96:4 by weight, control moisture within 15%, get product under 30 DEG C of conditions.

Further, described step 2) be specially: complex microorganism component and tap water, dextrin are sprayed to composite organic-inorganic fertilizer semi-finished granules surface after mixing with the ratio of 5:12:10, after mixing, dry under 30 DEG C of conditions and control moisture below 15%, get product.

Further, the preparation of described complex microorganism component comprises:

I. the preparation of bacillus category spore powder:

A. slant culture: the original strain of subtilis and bacillus megaterium is aseptically inoculated on slant medium respectively, cultivates 36-48 hour under 28 ± 2 DEG C of conditions;

B. shaking table is cultivated: be aseptically inoculated in respectively in seed culture medium by the bacterial classification that step a cultivates, and under pH6.5-7.0,30 DEG C of conditions, 12-18 hour cultivated by 140-160r/min shaking table;

C. fermentor cultivation: the bacterial classification that step b cultivates aseptically is inoculated in liquid fermentation medium respectively, pH7.0-7.5, tank pressure 0.5kg, 30 DEG C, under ventilation 1:0.8-1.1 condition, cultivate after 33-40 hour, viable count is greater than 1.0 × 10 10cfu/mL, 80% thalline becomes gemma tank at present, obtains fermented liquid;

D. obtain in step c two kinds of fermented liquids are prepared into Bacillus globigii spores powder and bacillus megaterium spore powder respectively through concentrate drying.

Wherein, the formula of the slant medium used in step a is as follows: glucose 15g, fish peptone 5g, yeast extract paste 5g, agar 15g and water 1000mL; The seed culture based formulas used in step b is as follows: glucose 10g, extractum carnis 5g, yeast powder 5g, starch 10g, soybean cake powder 5g, potassium primary phosphate 0.5g, magnesium sulfate 0.2g and water 1000mL; The liquid fermentation medium formula used in step c is as follows: Semen Maydis powder 26kg, soybean cake powder 16kg, ammonium sulfate 4kg, glucose 8kg, yeast powder 2.5kg, peptone 1.7kg and defoamer 50mL, add water to 600L.

II. the preparation of trichoderma harziarum spore powder:

E. trichoderma harziarum original strain is aseptically inoculated on slant medium, cultivates 48 hours under 28 ± 2 DEG C of conditions;

F. shaking table is cultivated: be aseptically inoculated in seed culture medium by the bacterial classification that step e cultivates, and under pH6.5-6.8,30 DEG C of conditions, 160-200r/min shaking table is cultivated about 24 hours;

G. fermentor cultivation: the bacterial classification that step f cultivates aseptically is inoculated in liquid fermentation medium, be 1:0.6-0.8 in pH6.5-6.8, tank pressure 0.5kg, 27-28 DEG C, ventilation, after cultivation about 48 hours, mycelium stops fermentation when accounting for 20% of cumulative volume, carries out solid fermentation and produces spore;

H.. solid fermentation produces spore: be inoculated on solid fermentation substratum by the mycelium after step g by fermentation tank cultivation, cultivate 144-168 hour, when trichoderma harziarum 90% produces spore, stop fermentation;

I. the fermentation materials producing spore in step h is completely immersed in clear water and makes spore suspension, thus obtain trichoderma harziarum spore suspension;

J. the trichoderma harziarum spore suspension concentrate drying obtained in above-mentioned steps i is made spore powder.

Wherein, the formula of the slant medium used in step e is as follows: glucose 20g, murphy juice 200g, agar 20g and water 1000mL, pH nature; The seed culture based formulas used in step f is as follows: white sugar 20g, yeast extract paste 0.5g, starch 20g, potassium primary phosphate 0.5g, magnesium sulfate 0.2g, sodium-chlor 0.2g and water 1000mL; The liquid fermentation medium formula used in step g is as follows: starch 12kg, soybean cake powder 1.2kg, Semen Maydis powder 3kg, white sugar 12kg, yeast extract paste 0.3kg, magnesium sulfate 0.12kg, sodium-chlor 0.12kg, defoamer 50mL, add water to 600L; Solid fermentation culture medium prescription in step h is as follows: cotton seed hulls, Semen Maydis powder and wheat bran add water by the solid material of 5:2:13 weight ratio mixing composition, and obtain solid fermentation substratum, the weight ratio of solid material and water is 1:0.6.

III. the preparation of Streptomyces hygrospinosus bacterium powder:

K., aseptically, by Streptomyces hygrospinosus strain inoculation on slant medium, at 28 DEG C, 5-6 days is cultivated;

L. shaking table is cultivated: be aseptically inoculated in seed culture medium by the bacterial classification that step k cultivates, and under 28 DEG C of conditions, 220r/min shaking table is cultivated about 24 hours;

M. fermentor cultivation: aseptically, in the bacterial classification access liquid fermentation medium that step l is cultivated, at initial pH7.5, tank pressure 0.5kg, 28 DEG C, ventilation 1:0.5-1, cultivate after 48 hours, put tank and collect fermented liquid, obtained spore powder after concentrate drying.

Wherein, the formula of the slant medium used in step k is as follows: Zulkovsky starch 20g, saltpetre 1g, sodium-chlor 0.5g, dipotassium hydrogen phosphate 0.5g, magnesium sulfate 0.5g, FeSO 40.1g, agar powder 18g and distilled water 1000mL, pH7.2-7.4; The culture medium prescription used in step l, m is as follows: W-Gum 20g, bean powder 10g, sodium-chlor 0.5g, dipotassium hydrogen phosphate 0.5g, ammonium sulfate 5g, calcium carbonate 3g, defoamer 0.5g and distilled water 1000mL, pH7.5.

IV. above-mentioned obtained Bacillus globigii spores powder, Bacillus megatherium spore powder, trichoderma harziarum spore powder, Streptomyces hygrospinosus bacterium powder are mixed by a certain percentage, obtain complex microorganism component, subtilis, bacillus megaterium in this component, the living bacteria count ratio of trichoderma harziarum, Streptomyces hygrospinosus is 4: 2: 1: 1.

As further illustrating, above-mentioned substratum all adopts high pressure steam sterilization, namely at 121 DEG C, and sterilizing 30min under 0.1Mpa condition.

Further, the preparation method of described fertilizer component is:

Dregs of beans is mixed in mass ratio with rapeseed meal, flue-cured tobacco straw powder at 6: 3: 1, about regulating moisture to 60%, piles high 1.5 meters, stack retting 40 ~ 60 days; Coating film when fermenting initial, film can be removed when fermentation heap core temperature is more than 50 DEG C, then need to use stack turner turning when fermentation heap core temperature is more than 65 DEG C, ferment and suitably keep the skin wet for first 20 days, make fermentation heap moisture maintain in the scope of 45% ~ 55%; Repeatable operation like this, until fermentation heap temperature drops to about 40 DEG C, color becomes beige, no foul smell, fermentation ends when moisture is below 30%, obtains the fertilizer component of becoming thoroughly decomposed.

Beneficial effect of the present invention is:

The present invention is a kind of biological, organic, the Chemical Mixed Fertilizer of inorganic triplicity, containing four kinds of rice disease biological control agentses in product, and living bacteria count is up to 2-18 hundred million cfu/g, inorganic total nutrient reaches 20-33%, organic up to 25%, this invention is by the synergy of bio-fertilizer, the steady effect of short effect effect and fertilizer, the quick-acting effects of long-acting and inorganic fertilizer be combined with each other, the full nutrition needed for paddy growth can be met, improve rice quality, realize increasing both production and income, soil fertility of paddy field can be improved simultaneously, improve paddy soil environment, effectively prevent and treat the generation of rice disease.Relative to other like products, the present invention adopts dextrin parcel, cryodrying technology, can avoid occurring microorganism mortality situation in fertilizer and microorganism granulation process, effectively extend the storage life of microorganism.Simultaneously, in bacterial classification selection, we have selected targetedly and effectively can have again the bacterial strain of biological and ecological methods to prevent plant disease, pests, and erosion function concurrently by Promoting plant growth, screening through this center is tamed, it is long that bacterial strain has the shelf time, and field colonization ability is strong, the features such as reproduction speed is fast, and this product effective bacterium number content is high, can guarantee that microorganism can rapid field planting act on paddy rice in field.

Embodiment

Following examples for illustration of the present invention, but are not used for limiting the scope of the invention.

The preparation method of embodiment 1 one kinds of paddy rice Special compound microbial fertilizers

This paddy rice Special compound microbial fertilizer is containing following parts by weight material:

Composite organic-inorganic fertilizer work in-process 96 parts;

Complex microorganism component 4 parts.

Described composite organic-inorganic fertilizer work in-process are containing the raw material of following parts by weight:

The formation of described complex microorganism living bacteria count content is:

(1) preparation of above-mentioned complex microorganism component:

I. the preparation of genus bacillus mixing spore powder:

A. slant culture: the original strain of subtilis and bacillus megaterium is aseptically inoculated on slant medium respectively, cultivates 36-48 hour under 28 ± 2 DEG C of conditions;

B. shaking table is cultivated: be aseptically inoculated in respectively in seed culture medium by the bacterial classification that step a cultivates, and under pH6.5-7.0,30 DEG C of conditions, 12-18 hour cultivated by 140-160r/min shaking table;

C. fermentor cultivation: the bacterial classification that step b cultivates aseptically is inoculated in liquid fermentation medium respectively, pH7.0-7.5, tank pressure 0.5kg, 30 DEG C, under ventilation 1:0.8-1.1 condition, cultivate after 33-40 hour, viable count is greater than 1.0 × 10 9cfu/mL, 80% thalline becomes gemma tank at present, obtains fermented liquid;

D. obtain in step c two kinds of fermented liquids are prepared into Bacillus globigii spores powder and bacillus megaterium spore powder respectively through concentrate drying.

Wherein, the formula of the slant medium used in step a is as follows: glucose 15g, fish peptone 5g, yeast extract paste 5g, agar 15g and water 1000mL; The seed culture based formulas used in step b is as follows: glucose 10g, extractum carnis 5g, yeast powder 5g, starch 10g, soybean cake powder 5g, potassium primary phosphate 0.5g, magnesium sulfate 0.2g and water 1000mL; The liquid fermentation medium formula used in step c is as follows: Semen Maydis powder 26kg, soybean cake powder 16kg, ammonium sulfate 4kg, glucose 8kg, yeast powder 2.5kg, peptone 1.7kg and defoamer 50mL, add water to 600L.

II. the preparation of trichoderma harziarum spore powder:

E. trichoderma harziarum original strain is aseptically inoculated on slant medium, cultivates 48 hours under 28 ± 2 DEG C of conditions;

F. shaking table is cultivated: be aseptically inoculated in seed culture medium by the bacterial classification that step e cultivates, and under pH6.5-6.8,30 DEG C of conditions, 160-200r/min shaking table is cultivated about 24 hours;

G. fermentor cultivation: the bacterial classification that step f cultivates aseptically is inoculated in liquid fermentation medium, be 1:0.6-0.8 in pH6.5-6.8, tank pressure 0.5kg, 27-28 DEG C, ventilation, after cultivation about 48 hours, mycelium stops fermentation when accounting for 20% of cumulative volume, carries out solid fermentation and produces spore;

H.. solid fermentation produces spore: be inoculated on solid fermentation substratum by the mycelium after step g by fermentation tank cultivation, cultivate 144-168 hour, when trichoderma harziarum 90% produces spore, stop fermentation;

I. the fermentation materials producing spore in step h is completely immersed in clear water and makes spore suspension, thus obtain trichoderma harziarum spore suspension;

J. the trichoderma harziarum spore suspension concentrate drying obtained in above-mentioned steps i is made spore powder.

Wherein, the formula of the slant medium used in step e is as follows: glucose 20g, murphy juice 200g, agar 20g and water 1000mL, pH nature; The seed culture based formulas used in step f is as follows: white sugar 20g, yeast extract paste 0.5g, starch 20g, potassium primary phosphate 0.5g, magnesium sulfate 0.2g, sodium-chlor 0.2g and water 1000mL; The liquid fermentation medium formula used in step g is as follows: starch 12kg, soybean cake powder 1.2kg, Semen Maydis powder 3kg, white sugar 12kg, yeast extract paste 0.3kg, magnesium sulfate 0.12kg, sodium-chlor 0.12kg, defoamer 50mL, add water to 600L; Solid fermentation culture medium prescription in step h is as follows: cotton seed hulls, Semen Maydis powder and wheat bran add water by the solid material of 5:2:13 weight ratio mixing composition, and obtain solid fermentation substratum, the weight ratio of solid material and water is 1:0.6.

III. the preparation of Streptomyces hygrospinosus bacterium powder:

K., aseptically, by Streptomyces hygrospinosus strain inoculation on slant medium, at 28 DEG C, 5-6 days is cultivated;

L. shaking table is cultivated: be aseptically inoculated in seed culture medium by the bacterial classification that step k cultivates, and under 28 DEG C of conditions, 220r/min shaking table is cultivated about 24 hours;

M. fermentor cultivation: aseptically, in the bacterial classification access liquid fermentation medium that step l is cultivated, at initial pH7.5, tank pressure 0.5kg, 28 DEG C, ventilation 1:0.5-1, cultivate after 48 hours, put tank and collect fermented liquid, obtained spore powder after concentrate drying.

Wherein, the formula of the slant medium used in step k is as follows: Zulkovsky starch 20g, saltpetre 1g, sodium-chlor 0.5g, dipotassium hydrogen phosphate 0.5g, magnesium sulfate 0.5g, FeSO 40.1g, agar powder 18g and distilled water 1000mL, pH7.2-7.4; The culture medium prescription used in step l, m is as follows: W-Gum 20g, bean powder 10g, sodium-chlor 0.5g, dipotassium hydrogen phosphate 0.5g, ammonium sulfate 5g, calcium carbonate 3g, defoamer 0.5g and distilled water 1000mL, pH7.5.

IV. above-mentioned obtained genus bacillus mixing spore powder, trichoderma harziarum spore powder, Streptomyces hygrospinosus bacterium powder are mixed by a certain percentage, obtain complex microorganism component, the total viable count content of complex microorganism is 30,000,000,000 cfu/g herein.Subtilis, bacillus megaterium in this component, the living bacteria count ratio of trichoderma harziarum, Streptomyces hygrospinosus is 4: 2: 1: 1.

Above-mentioned substratum all adopts high pressure steam sterilization, namely at 121 DEG C, and sterilizing 30min under 0.1Mpa condition;

(2) preparation of fertilizer component:

Dregs of beans is mixed in mass ratio with rapeseed meal, flue-cured tobacco straw powder at 6: 3: 1, about regulating moisture to 60%, piles high 1.5 meters, stack retting 40 ~ 60 days.Coating film when fermenting initial, film can be removed when fermentation heap core temperature is more than 50 DEG C, then need to use stack turner turning when fermentation heap core temperature is more than 65 DEG C, ferment and suitably keep the skin wet for first 20 days, fermentation heap moisture is maintained in the scope of 45% ~ 55%.Repeatable operation like this, until fermentation heap temperature drops to about 40 DEG C, color becomes beige, no foul smell, when moisture is below 30% and fermentation ends, obtains the fertilizer component of becoming thoroughly decomposed.

(3) the half-finished preparation of composite organic-inorganic fertilizer

Fertilizer component, inorganic composite fertilizer component, wilkinite are mixed according to a certain percentage, are a granulated into the work in-process of the particle of particle diameter 4mm, control moisture about 12%, these work in-process are nitrogenous 14%, phosphorus 5%, potassium 9%, organic 25%.

(4) preparation of paddy rice Special compound microbial fertilizer:

By weight 96 parts of composite organic-inorganic fertilizer work in-process are mixed with 4 parts of complex microorganism components, under 30 DEG C of conditions, control moisture within 15%, paddy rice Special compound microbial fertilizer finished product.Concrete steps are as follows: by 4 parts of complex microorganism components and tap water, dextrin is to be sprayed to above-mentioned composite organic-inorganic fertilizer semi-finished granules surface with shotcrete machine after the mixing of the ratio of 5:12:10, after mixing, dry under 30 DEG C of conditions and control moisture in 15%, namely obtain this product paddy rice Special compound microbial fertilizer, the content of its each effective constituent is: nitrogen 14%, phosphorus 5%, potassium 9%, organic matter 25%, complex microorganism number content 1,200,000,000 cfu/g.

The preparation method of embodiment 2 one kinds of paddy rice Special compound microbial fertilizers

This paddy rice Special compound microbial fertilizer is containing following parts by weight material:

Composite organic-inorganic fertilizer work in-process 96 parts;

Complex microorganism component 4 parts.

Described composite organic-inorganic fertilizer work in-process are containing the raw material of following parts by weight:

The formation of described complex microorganism living bacteria count content:

When preparing complex microorganism combination, its total living bacteria count content is adjusted to 45,000,000,000 cfu/g, then with tap water, dextrin is to be sprayed to composite organic-inorganic fertilizer semi-finished granules surface with shotcrete machine after the mixing of the ratio of 5:12:10.All the other methods are with embodiment 1.

This product paddy rice Special compound microbial fertilizer, the content of its each effective constituent is: nitrogen 15%, phosphorus 8%, potassium 10%, organic matter 25%, complex microorganism number content 1,800,000,000 cfu/g.

Embodiment 3 paddy rice Special compound microbial fertilizer

This paddy rice Special compound microbial fertilizer is containing following parts by weight material:

Composite organic-inorganic fertilizer work in-process 96 parts;

Complex microorganism component 4 parts.

Described composite organic-inorganic fertilizer work in-process are containing the raw material of following parts by weight:

The formation of above-mentioned complex microorganism living bacteria count content:

When preparing complex microorganism combination, its total living bacteria count content is adjusted to 5,000,000,000 cfu/g, then with tap water, dextrin is to be sprayed to composite organic-inorganic fertilizer semi-finished granules surface with shotcrete machine after the mixing of the ratio of 5:12:10.All the other methods are with embodiment 1.

This product paddy rice Special compound microbial fertilizer, the content of its each effective constituent is: nitrogen 10%, phosphorus 4%, potassium 6%, organic matter 25%, complex microorganism number content 200,000,000 cfu/g.

The potted plant manure trial of test example 1 one kinds of paddy rice Special compound microbial fertilizers

One, test objective

Potted plant simultaneous test is carried out to the application of paddy rice Special compound microbial fertilizer of the present invention, verifies effect of the present invention.

Two, test design

For examination paddy rice: Jin You 297.

For examination soil: ortho-water paddy soil, its basic physical and chemical is pH5.3, organic 20.2g/kg, full nitrogen 1.02g/Kg, full phosphorus 0.41g/Kg, full potassium 24.5g/Kg.

Test method: test arranges 4 process, each process 3 is parallel, each process adopts following fertilizer as base manure respectively: process 1: adopt embodiment 1 paddy rice Special compound microbial fertilizer (nitrogen 14%, phosphorus 5%, potassium 9%, organic matter 25%, complex microorganism number content 1,200,000,000 cfu/g), total amount of application 100Kg/ mu; Process 2: adopt the paddy rice Special compound microbial fertilizer through Micvowave Sterilization process (nitrogen 14%, phosphorus 5%, potassium 9%, organic 25%) in embodiment 1, total amount of application 100Kg/ mu; Process 3: adopt peasant household's conventional fertilizer application method; Process 4: do not apply fertilizer.

Adopt pot experiment, select 60cm × 100cm plastic tub, the wind desiceted soil 20Kg of in-built mistake 10 mesh sieve, 45 root and stem of certain plants inserted by every basin, and every root and stem of certain plants inserts 2 seedlings, and spacing upper and lower about seedling is 10cm, by the management of Rice Cropping ordinary method, by basin results species test product after paddy rice maturation.

Three, test-results

The different base manure process of table 1 is on the impact of rice biological proterties

Known by test-results, the paddy growth situation using paddy rice Special compound microbial fertilizer from average plant height, average spike length, spike number, examples explain, each measurement index analysis of thousand seed weight obviously than through the paddy rice Special compound microbial fertilizer of Micvowave Sterilization process, peasant household's conventional fertilizer application method and the production effect that do not apply fertilizer good.

Test example 2

One, test objective

Community simultaneous test is carried out to the application of paddy rice Special compound microbial fertilizer of the present invention, verifies effect of the present invention.

Two, test design

Field experiment was in 2012, and we choose and are positioned at group one piece of Rice Cropping field, Cha Zi mountain, Ya Tou village, Chun Kou town, Liuyang City of Hunan Province, this field area 3 mu, its physiochemical properties of soil: pH5.1, organic 27.2g/kg, full nitrogen 1.5g/Kg, full phosphorus 0.71g/Kg, full potassium 15.3g/Kg.

Experimental plot is divided into 4 process, each process 3 communities, Gong12Ge community, and each process adopts following fertilizer to apply fertilizer respectively:

Process 1: adopt the embodiment of the present invention 1 paddy rice Special compound microbial fertilizer (nitrogen 14%, phosphorus 5%, potassium 9%, organic matter 25%, complex microorganism number content 1,200,000,000 cfu/g), amount of application 100Kg/ mu;

Process 2: adopt the paddy rice Special compound microbial fertilizer through Micvowave Sterilization process (nitrogen 14%, phosphorus 5%, potassium 9%, organic 25%) in embodiment 1, total amount of application 100Kg/ mu;

Process 3: peasant household's conventional fertilizer application method, family pig manure 150Kg/ mu, potash fertilizer 15Kg/ mu, composite fertilizer's (Nitrogen-and Phosphorus-containing potassium) 25Kg/ mu, urea 20Kg/ mu.

Process 4: do not apply fertilizer.

Field management, to topdress and the prevention and control of plant diseases, pest control: undertaken by Rice Cropping usual manner, suitably supplement the trace element fertilizers such as zinc, boron, silicon according to paddy growth situation.

Three, test-results

The process of table 2 different fertilizer is on the impact of rice yield constituent element

Known by test-results, the paddy growth situation of paddy rice Special compound microbial fertilizer is used from spike number, every total grain panicle number in whole production process, Defined daily doses, thousand seed weight and each measurement index analysis of last ultimate production see that the fertilizer efficiency of paddy rice Special compound microbial fertilizer of the present invention is better than peasant household's conventional fertilizer application method.Meanwhile, the fertilizer efficiency of paddy rice Special compound microbial fertilizer of the present invention is also better than the composite microbiological fertilizer after sterilizing, and this illustrates that microbe composition of the present invention has reasonable effect to increasing production of rice.

The process of table 3 different fertilizer is to rice pest preventive effect

Prevent and treat in time when occurring on a large scale with fooled disease and pest.From the results of view, paddy rice Special compound microbial fertilizer of the present invention can well prevent and treat bacterium and fungal disease, has reasonable prevention effect.

Although above the present invention is described in detail with a general description of the specific embodiments, on basis of the present invention, can make some modifications or improvements it, this will be apparent to those skilled in the art.Therefore, these modifications or improvements without departing from theon the basis of the spirit of the present invention, all belong to the scope of protection of present invention.

Claims (7)

1. a paddy rice Special compound microbial fertilizer, is characterized in that, this fertilizer consists of the following composition: fertilizer component, inorganic composite fertilizer component, wilkinite and complex microorganism component; Contain in described composite microbiological fertilizer: nitrogen 10-15%, phosphorus 4-8%, potassium 6-10%, organic matter 25%, complex microorganism number content 2-18 hundred million cfu/g;
The preparation method of described paddy rice Special compound microbial fertilizer comprises the following steps:
1) fertilizer component, inorganic composite fertilizer component, wilkinite are mixed in the ratio of 33.3:35.1 ~ 62.4:0.3 ~ 27.6, be a granulated into the particle of particle diameter 4mm, control moisture about 12%, obtain the composite organic-inorganic fertilizer work in-process of nitrogenous 10-15%, phosphorus 4-8%, potassium 6-10%, organic matter 25%;
2) by step 1) the composite organic-inorganic fertilizer work in-process that obtain and complex microorganism component by weight for the ratio of 96:4 mixes, control moisture within 15% under 30 DEG C of conditions, get product;
Described complex microorganism component is made up of subtilis, bacillus megaterium, trichoderma harziarum and Streptomyces hygrospinosus four kinds of microorganisms; The living bacteria count ratio of subtilis in described complex microorganism component, bacillus megaterium, trichoderma harziarum and Streptomyces hygrospinosus is 4: 2: 1: 1.
2. composite microbiological fertilizer according to claim 1, is characterized in that, described fertilizer component is formed by dregs of beans, rapeseed meal, flue-cured tobacco straw powder compost fermentation; Wherein the mass ratio of dregs of beans, rapeseed meal, flue-cured tobacco straw powder is 6: 3: 1.
3. composite microbiological fertilizer according to claim 1, is characterized in that, described inorganic composite fertilizer component is made up of urea, potassium sulfate and calcium superphosphate.
4. composite microbiological fertilizer according to claim 3, is characterized in that, described inorganic composite fertilizer component is made up of 46% urea, 45% potassium sulfate and 53% calcium superphosphate;
Wherein the mass ratio of 46% urea, 45% potassium sulfate and 53% calcium superphosphate is 18.8 ~ 29.7: 8.1 ~ 17: 8.2 ~ 15.7.
5. the preparation method of the composite microbiological fertilizer described in claim 1-4 any one, is characterized in that, said method comprising the steps of:
1) fertilizer component, inorganic composite fertilizer component, wilkinite are mixed in the ratio of 33.3:35.1 ~ 62.4:0.3 ~ 27.6, be a granulated into the particle of particle diameter 4mm, control moisture about 12%, obtain the composite organic-inorganic fertilizer work in-process of nitrogenous 10-15%, phosphorus 4-8%, potassium 6-10%, organic matter 25%;
2) by step 1) the composite organic-inorganic fertilizer work in-process that obtain and complex microorganism component by weight for the ratio of 96:4 mixes, control moisture within 15% under 30 DEG C of conditions, get product;
The preparation of described complex microorganism component comprises:
I. the preparation of bacillus category spore powder: after subtilis and bacillus megaterium original strain aseptically being carried out respectively successively slant culture, shaking table cultivation, fermentor cultivation, the fermentation liquor obtained is crossed concentrate drying and be prepared into Bacillus globigii spores powder and bacillus megaterium spore powder;
II. the preparation of trichoderma harziarum spore powder: after trichoderma harziarum original strain aseptically being carried out successively slant culture, shaking table cultivation, fermentor cultivation, solid fermentation product spore, the substratum producing spore completely obtained is immersed in clear water and makes spore suspension, thus obtain trichoderma harziarum spore suspension, then the trichoderma harziarum spore suspension concentrate drying obtained is prepared into trichoderma harziarum spore powder;
III. the preparation of Streptomyces hygrospinosus bacterium powder: after Streptomyces hygrospinosus original strain aseptically being carried out successively slant culture, shaking table cultivation, fermentor cultivation, be prepared into Streptomyces hygrospinosus bacterium powder through concentrate drying;
IV. the ratio being 4: 2: 1: 1 by above-mentioned obtained Bacillus globigii spores powder, Bacillus megatherium spore powder, trichoderma harziarum spore powder, Streptomyces hygrospinosus bacterium powder according to its living bacteria count ratio mixes composite, obtains complex microorganism component.
6. preparation method according to claim 5, it is characterized in that, described step 2) be specially: complex microorganism component and tap water, dextrin are sprayed to composite organic-inorganic fertilizer semi-finished granules surface after mixing with the ratio of 5:12:10, after mixing, dry under 30 DEG C of conditions and control moisture below 15%, get product.
7. preparation method according to claim 5, is characterized in that, the preparation method of described fertilizer component is:
Dregs of beans is mixed in mass ratio with rapeseed meal, flue-cured tobacco straw powder at 6: 3: 1, about regulating moisture to 60%, piles high 1.5 meters, stack retting 40 ~ 60 days; Coating film when fermenting initial, film can be removed when fermentation heap core temperature is more than 50 DEG C, then need to use stack turner turning when fermentation heap core temperature is more than 65 DEG C, ferment and suitably keep the skin wet for first 20 days, make fermentation heap moisture maintain in the scope of 45% ~ 55%; Repeatable operation like this, until fermentation heap temperature drops to about 40 DEG C, color becomes beige, no foul smell, fermentation ends when moisture is below 30%, obtains the fertilizer component of becoming thoroughly decomposed.
CN201410158319.7A 2014-04-18 2014-04-18 A kind of paddy rice Special compound microbial fertilizer and preparation method thereof CN103964946B (en)

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