CN107337517A - It is a kind of to utilize long-acting organic biofertilizer of bean pulp fermentation and preparation method thereof - Google Patents

It is a kind of to utilize long-acting organic biofertilizer of bean pulp fermentation and preparation method thereof Download PDF

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CN107337517A
CN107337517A CN201710549284.3A CN201710549284A CN107337517A CN 107337517 A CN107337517 A CN 107337517A CN 201710549284 A CN201710549284 A CN 201710549284A CN 107337517 A CN107337517 A CN 107337517A
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parts
strain
flora
cfu
nutrient solution
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江凯
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Guangxi Sailing Investment Co Ltd
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Guangxi Sailing Investment Co Ltd
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    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05CNITROGENOUS FERTILISERS
    • C05C11/00Other nitrogenous fertilisers
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity

Abstract

The invention discloses a kind of long-acting organic biofertilizer using bean pulp fermentation, the long-acting organic biofertilizer using bean pulp fermentation is mainly made up of following parts by weight material:50 100 parts of dregs of beans, 30 50 parts of mushroom dreg, 30 50 parts of soy sauce waste liquid, 8 12 parts of seawood meal, 8 15 parts of shrimp shell meal, 10 15 parts of oyster shell whiting, 20 30 parts of attapulgite, 7 10 parts of tea seed cake, 20 30 parts of cassava grain stillage, 20 30 parts of corncob, 58 parts of Cortex Meliae extract, 15 parts of chelating amino acids agent, 15 parts of strain, 58 parts of chitosan, 50 100 parts of starch, 5 10 parts of sesbania gum, 20 25 parts of sodium alginate;The strain fermentation that the present invention was tamed as the main fermented ingredient of organic fertilizer using dregs of beans, mushroom dreg, soy sauce waste liquid with other materials by the present invention, mutually acts synergistically and enough nutritional ingredients is provided to plant.

Description

It is a kind of to utilize long-acting organic biofertilizer of bean pulp fermentation and preparation method thereof
Technical field
The present invention relates to technical field of fertilizers.It is more particularly related to a kind of have using the long-acting of bean pulp fermentation Machine bio-bacterial manure and preparation method thereof.
Background technology
Fertilizer, it is to provide nutrient necessary to one or more kinds of plants, improves soil property, increases soil fertility Horizontal a kind of material.Bio-bacterial manure is the one kind commonly used in fertilizer, and microbial manure is caused with the vital movement of microorganism Crop obtains a kind of product of specific fertilizer effect, and microbial manure is live body fertilizer, and its effect contains big mainly by it Beneficial microorganism vital movement is measured to complete.Only when these beneficial microbes are in vigorous breeding and metabolic feelings Under condition, material conversion and beneficial metabolic product could be formed constantly.Therefore, beneficial microorganism species, life in microbial manure It is the basis of its validity that whether life activity vigorous, is in the form of the essential elements such as nitrogen, phosphorus, potassium and more rather than other fertilizer Based on few.Just because of microbial manure is preparation living, so its fertilizer efficiency and number of viable, intensity and ambient environmental conditions are close Correlation, including temperature, moisture, acid-base value, nutritional condition and original are lived in indigenous microorganism repulsive interaction in soil and had necessarily Influence, therefore to be noted that in application.
The content of the invention
Present invention aim to address above mentioned problem, there is provided a kind of cost ineffective fruit significantly utilizes the long-acting of bean pulp fermentation Organic biofertilizer, the present invention use the main fermented ingredient and other materials of dregs of beans, mushroom dreg, soy sauce waste liquid as organic fertilizer The strain fermentation that material was tamed by the present invention, mutually act synergistically and enough nutritional ingredients, wherein most are provided to plant Organic matter, which is absorbed by plants, is converted into the organic matter of plant in itself;
The present invention also provides a kind of preparation method of the long-acting organic biofertilizer using bean pulp fermentation, by the way that material is passed through Cross irradiation field to irradiate so that organic matter therein is easily absorbed by plants, significantly improve the utilization rate of fertilizer, starch, amino acid chela Mixture, chitosan, sesbania gum, sodium alginate hybrid energy form multilayer long-acting slow-release diaphragm, extend the usage time of fertilizer.
Technical scheme provided by the invention is:
A kind of long-acting organic biofertilizer using bean pulp fermentation, the long-acting organic biofertilizer using bean pulp fermentation Mainly it is made up of following parts by weight material:Dregs of beans 50-100 parts, mushroom dreg 30-50 parts, soy sauce waste liquid 30-50 parts, seawood meal 8-12 parts, shrimp shell meal 8-15 parts, oyster shell whiting 10-15 parts, attapulgite 20-30 parts, tea seed cake 7-10 parts, cassava grain stillage 20-30 parts, Corncob 20-30 parts, Cortex Meliae extract 5-8 parts, chelating amino acids agent 1-5 parts, strain 1-5 parts, chitosan 5-8 parts, starch 50-100 parts, sesbania gum 5-10 parts, sodium alginate 20-25 parts;Wherein, the strain consists of:Saccharomycete, gemma bar Bacterium, streptomycete, nitrogen-fixing bacteria, lactic acid bacteria, each strain are that 1: 1: 0.2: 0.5: 0.8 mixing forms according to weight ratio, the bacterium of saccharomycete Group's number is 10 × 106-15×106CFU/g, the flora number of bacillus is 8 × 106-10×106CFU/g, the flora of streptomycete Number is 6 × 106-12×106CFU/g, the flora number of nitrogen-fixing bacteria is 12 × 106-15×106CFU/g, the flora number of lactic acid bacteria is 8 ×106-9×106CFU/g。
Preferably, the long-acting organic biofertilizer using bean pulp fermentation is mainly made up of following parts by weight material: 80 parts of dregs of beans, 35 parts of mushroom dreg, 45 parts of soy sauce waste liquid, 10 parts of seawood meal, 12 parts of shrimp shell meal, 12 parts of oyster shell whiting, attapulgite 25 parts, 8 parts of tea seed cake, 25 parts of cassava grain stillage, 25 parts of corncob, 7 parts of Cortex Meliae extract, 4 parts of chelating amino acids agent, 3 parts of strain, 7 parts of chitosan, 85 parts of starch, 8 parts of sesbania gum, 23 parts of sodium alginate.
Preferably, the saccharomycete, bacillus, streptomycete, nitrogen-fixing bacteria, lactic acid bacteria are tamed and dociled by following steps respectively Change:Saccharomycete, bacillus, streptomycete, nitrogen-fixing bacteria, lactic acid bacteria are moved into the first blake bottle addition nutrient solution, nutrient solution respectively Preparation:Sucrose containing 20-50% mass fractions, 1% mass fraction potassium chloride, 10% mass fraction gelatinized starch, surplus For water, nutrient solution PH is adjusted to 4-5, temperature is -10 to 0 degree, is cultivated 2-5 days;The strain that lives is moved to second training respectively Support in bottle and add nutrient solution again, nutrient solution PH is adjusted to 5-6, temperature is 0 to 10 degree, is cultivated 2-5 days;The strain that will live is distinguished Move to and add new nutrient solution in the 3rd blake bottle again, nutrient solution PH is adjusted to 6-7, temperature is 10 to 20 degree, cultivates 2-5 My god;The strain that will live moves in the 4th blake bottle respectively adds new nutrient solution again, and nutrient solution PH is adjusted to 7-8, temperature For 20-30 degree, cultivate 2-5 days;The strain that will live moves in the 5th blake bottle respectively adds new nutrient solution again, by nutrient solution PH is adjusted to 7-8, and temperature is 30-40 degree, is cultivated 3-7 days, adjusts each flora number to following scope after purifies and separates:Saccharomycete Flora number be 10 × 106-15×106CFU/g, the flora number of bacillus is 8 × 106-10×106CFU/g, streptomycete Flora number is 6 × 106-12×106CFU/g, the flora number of nitrogen-fixing bacteria is 12 × 106-15×106CFU/g, the flora of lactic acid bacteria Number is 8 × 106-9×106CFU/g, obtain the saccharomycete, bacillus, streptomycete, nitrogen-fixing bacteria, lactic acid bacteria;Given birth to for strain Than more sensitive two Conditions Temperatures and PH for length, after the present invention is by strain substep culture domestication, strain adapts to various PH Temperature survives, and after repurity separation, obtains the resistance to strain for freezing heat-resisting acid-fast alkali-proof, is adapted to plant throughout the year each a variety of Environment is planted, is advantageous to the growth of plant.
The present invention also provides a kind of preparation method of the described long-acting organic biofertilizer using bean pulp fermentation, the system Preparation Method comprises the following steps:
Step 1: by dregs of beans, mushroom dreg, soy sauce waste liquid, seawood meal, shrimp shell meal, oyster shell whiting, attapulgite, tea seed cake, wood High-speed stirred 1-2 hours after the water of potato vinasse, corncob and 2-3 times of volume is mixed, at the uniform velocity by irradiation field, irradiation dose For 30-50KGy, exposure time is the 10-20 seconds, obtains mixture A;
Step 2: mixture A is well mixed after fermentation 3-5 days with strain, by being dried to obtain fermentation material, by fermentation material 100 mesh sieve, which are crushed, after being mixed with Cortex Meliae extract, chelating amino acids agent obtains powder;
Step 3: the chitosan, starch, sesbania gum, sodium alginate are added into isometric water, after being heated to dissolving, Obtain mixing liquid B;
Step 4: mixing liquid B is mixed with the powder that step 2 obtains, after stirring, fertiliser granulates are made, dry Obtain the long-acting organic biofertilizer using bean pulp fermentation.
Effective time 3-6 month that the present invention uses.
The present invention comprises at least following beneficial effect:
The present invention is led to using dregs of beans, mushroom dreg, soy sauce waste liquid as the main fermented ingredient and other materials of organic fertilizer The strain fermentation that the present invention tamed is crossed, mutually acts synergistically and enough nutritional ingredients is provided to plant, wherein most is organic Matter, which is absorbed by plants, is converted into the organic matter of plant in itself, and the strain of addition of the invention passes through the low temperature resistant high temperature resistant of acid-fast alkali-proof Purifying obtains strain after domestication, and activity is adapted to well various growing environments, can not only by the organic matter decomposition in fertilizer into small molecule, Easily it is absorbed by plants, moreover it is possible to the organic matter in soil is decomposed, and there is fixed nitrogen, bacillus, the streptomycete of addition Synergy, the illness rate of root system of plant is significantly reduced, the present invention is mainly using starch as fertilizer slow release moisturizing carrier, cost It is low, the effect for playing that significantly sustained release moisturizing is long-acting is mixed with other auxiliary material chitosans, sesbania gum, sodium alginate, wherein beautiful Meter Xin acts not only as nutrient by processing and is absorbed by plants while can also be used as fertilizer NMF;
The present invention also provides a kind of preparation method of the long-acting organic biofertilizer using bean pulp fermentation, by the way that material is passed through Irradiation field irradiation is crossed again after everfermentation so that the organic matter in fertilizer is easily absorbed by plants the utilization rate for improving fertilizer, shallow lake Powder, chelating amino acids agent, chitosan, sesbania gum, sodium alginate combination drying can form multilayer long-acting slow-release diaphragm, extend fertilizer The usage time of material.
Further advantage, target and the feature of the present invention embodies part by following explanation, and part will also be by this The research and practice of invention and be understood by the person skilled in the art.
Embodiment
With reference to example below, the present invention is described in further detail, to make those skilled in the art with reference to specification Word can be implemented according to this.
Wherein 1 part of the embodiment of the present invention is 1kg
Embodiment 1
A kind of long-acting organic biofertilizer using bean pulp fermentation, mainly it is made up of following parts by weight material:50 parts of dregs of beans, 30 parts of mushroom dreg, 30 parts of soy sauce waste liquid, 8 parts of seawood meal, 8 parts of shrimp shell meal, 10 parts of oyster shell whiting, 20 parts of attapulgite, tea seed cake 7 Part, 20 parts of cassava grain stillage, 20 parts of corncob, 5 parts of Cortex Meliae extract, 1 part of chelating amino acids agent, 1 part of strain, 5 parts of chitosan, 50 parts of starch, 5 parts of sesbania gum, 20 parts of sodium alginate;Wherein, the strain consists of:Saccharomycete, bacillus, strepto- Bacterium, nitrogen-fixing bacteria, lactic acid bacteria, each strain are that 1: 1: 0.2: 0.5: 0.8 mixing forms according to weight ratio, and the flora number of saccharomycete is 10 ×106-15×106CFU/g, the flora number of bacillus is 8 × 106-10×106CFU/g, the flora number of streptomycete for 6 × 106-12×106CFU/g, the flora number of nitrogen-fixing bacteria is 12 × 106-15×106CFU/g, the flora number of lactic acid bacteria is 8 × 106-9 ×106CFU/g。
The preparation method of the described long-acting organic biofertilizer using bean pulp fermentation, comprises the following steps:
Step 1: by dregs of beans, mushroom dreg, soy sauce waste liquid, seawood meal, shrimp shell meal, oyster shell whiting, attapulgite, tea seed cake, wood High-speed stirred speed is 2000 turn/part 1 hour after the water of potato vinasse, corncob and 2 times of volumes is mixed, at the uniform velocity by irradiation , irradiation dose 30KGy, exposure time is 10 seconds, obtains mixture A;
Step 2: mixture A to be well mixed to after fermentation 3 days with strain, by being dried to obtain fermentation material, by fermentation material with 100 mesh sieve, which are crushed, after Cortex Meliae extract, chelating amino acids agent mixing obtains powder;
Step 3: the chitosan, starch, sesbania gum, sodium alginate are added into isometric water, after being heated to dissolving, Obtain mixing liquid B;
Step 4: mixing liquid B is mixed with the powder that step 2 obtains, after stirring, fertiliser granulates are made, dry Obtain the long-acting organic biofertilizer using bean pulp fermentation.
Embodiment 2
A kind of long-acting organic biofertilizer using bean pulp fermentation, mainly it is made up of following parts by weight material:Dregs of beans 100 Part, 50 parts of mushroom dreg, 50 parts of soy sauce waste liquid, 12 parts of seawood meal, 15 parts of shrimp shell meal, 15 parts of oyster shell whiting, 30 parts of attapulgite, tea 10 parts of the dregs of rice, 30 parts of cassava grain stillage, 30 parts of corncob, 8 parts of Cortex Meliae extract, 5 parts of chelating amino acids agent, 5 parts of strain, chitosan 8 parts, 100 parts of starch, 10 parts of sesbania gum, 25 parts of sodium alginate;Wherein, the strain consists of:Saccharomycete, gemma bar Bacterium, streptomycete, nitrogen-fixing bacteria, lactic acid bacteria, each strain are that 1: 1: 0.2: 0.5: 0.8 mixing forms according to weight ratio, the bacterium of saccharomycete Group's number is 10 × 106-15×106CFU/g, the flora number of bacillus is 8 × 106-10×106CFU/g, the flora of streptomycete Number is 6 × 106-12×106CFU/g, the flora number of nitrogen-fixing bacteria is 12 × 106-15×106CFU/g, the flora number of lactic acid bacteria is 8 ×106-9×106CFU/g。
Wherein, the saccharomycete, bacillus, streptomycete, nitrogen-fixing bacteria, lactic acid bacteria are tamed by following steps respectively:Will Saccharomycete, bacillus, streptomycete, nitrogen-fixing bacteria, lactic acid bacteria move to the first blake bottle addition nutrient solution, the system of nutrient solution respectively It is standby:Sucrose containing 20% mass fraction, 1% mass fraction potassium chloride, 10% mass fraction gelatinized starch, surplus are water, will It is -10 to 0 degree that nutrient solution PH, which is adjusted to 4-5, temperature, and PH is slowly heightened to threshold value, temperature and slowly raised during culture To threshold value, then stable pH value and temperature culture, co-culture 4 days;The strain that lives is moved in second blake bottle respectively and added again Nutrient solution, nutrient solution PH is adjusted to 5-6, temperature is 0 to 10 degree, and PH is slowly heightened to threshold value, temperature during culture Threshold value, then stable pH value and temperature culture are slowly raised to, is co-cultured 2 days;The strain that lives is moved to the 3rd blake bottle respectively In add new nutrient solution again, nutrient solution PH is adjusted to 6-7, temperature is 10 to 20 degree, and PH is slowly adjusted during culture Up to threshold value, temperature are slowly raised to threshold value, then stable pH value and temperature culture, co-cultured 5 days;The strain that will live moves to respectively New nutrient solution is added in 4th blake bottle again, nutrient solution PH is adjusted to 7-8, temperature is 20-30 degree, in the process of culture Middle PH is slowly heightened to threshold value, and temperature is slowly raised to threshold value, then stable pH value and temperature culture, co-cultures 3 days;To be lived bacterium Kind is moved in the 5th blake bottle and adds new nutrient solution again respectively, and nutrient solution PH is adjusted to 7-8, and temperature is 30-40 degree, PH is slowly heightened to threshold value during culture, and temperature is slowly raised to threshold value, then stable pH value and temperature culture, co-cultures 7 My god, each flora number is adjusted to following scope after purifies and separates:The flora number of saccharomycete is 10 × 106-15×106CFU/g, bud The flora number of spore bacillus is 8 × 106-10×106CFU/g, the flora number of streptomycete is 6 × 106-12×106CFU/g, nitrogen-fixing bacteria Flora number be 12 × 106-15×106CFU/g, the flora number of lactic acid bacteria is 8 × 106-9×106CFU/g, obtain the yeast Bacterium, bacillus, streptomycete, nitrogen-fixing bacteria, lactic acid bacteria;Wherein PH slowly heightens heightens 0.1 point for 1-2 hours, and temperature is slow Heighten and heighten 1 degree for 1-2 hours, the preferred PH of the present embodiment heightens for 1 hour at 0.1 point, and temperature heightens 1 degree in 1 hour.
The preparation method of the described long-acting organic biofertilizer using bean pulp fermentation, comprises the following steps:
Step 1: by dregs of beans, mushroom dreg, soy sauce waste liquid, seawood meal, shrimp shell meal, oyster shell whiting, attapulgite, tea seed cake, wood High-speed stirred 2 hours after the water of potato vinasse, corncob and 3 times of volumes is mixed, at the uniform velocity it is by irradiation field, irradiation dose 50KGy, exposure time are 20 seconds, obtain mixture A;
Step 2: mixture A to be well mixed to after fermentation 5 days with strain, by being dried to obtain fermentation material, by fermentation material with 100 mesh sieve, which are crushed, after Cortex Meliae extract, chelating amino acids agent mixing obtains powder;
Step 3: the chitosan, starch, sesbania gum, sodium alginate are added into isometric water, after being heated to dissolving, Obtain mixing liquid B;
Step 4: mixing liquid B is mixed with the powder that step 2 obtains, after stirring, fertiliser granulates are made, dry Obtain the long-acting organic biofertilizer using bean pulp fermentation.
Embodiment 3
A kind of long-acting organic biofertilizer using bean pulp fermentation, mainly it is made up of following parts by weight material:80 parts of dregs of beans, 35 parts of mushroom dreg, 45 parts of soy sauce waste liquid, 10 parts of seawood meal, 12 parts of shrimp shell meal, 12 parts of oyster shell whiting, 25 parts of attapulgite, tea seed cake 8 Part, 25 parts of cassava grain stillage, 25 parts of corncob, 7 parts of Cortex Meliae extract, 4 parts of chelating amino acids agent, 3 parts of strain, 7 parts of chitosan, 85 parts of starch, 8 parts of sesbania gum, 23 parts of sodium alginate;Wherein, the strain consists of:Saccharomycete, bacillus, strepto- Bacterium, nitrogen-fixing bacteria, lactic acid bacteria, each strain are that 1: 1: 0.2: 0.5: 0.8 mixing forms according to weight ratio, and the flora number of saccharomycete is 10 ×106-15×106CFU/g, the flora number of bacillus is 8 × 106-10×106CFU/g, the flora number of streptomycete for 6 × 106-12×106CFU/g, the flora number of nitrogen-fixing bacteria is 12 × 106-15×106CFU/g, the flora number of lactic acid bacteria is 8 × 106-9 ×106CFU/g。
Wherein, the saccharomycete, bacillus, streptomycete, nitrogen-fixing bacteria, lactic acid bacteria are tamed by following steps respectively:Will Saccharomycete, bacillus, streptomycete, nitrogen-fixing bacteria, lactic acid bacteria move to the first blake bottle addition nutrient solution, the system of nutrient solution respectively It is standby:Sucrose containing 20-50% mass fractions, 1% mass fraction potassium chloride, 10% mass fraction gelatinized starch, surplus are water, It is -10 to 0 degree that nutrient solution PH, which is adjusted to 4-5, temperature, and PH is heightened rapidly to threshold value, temperature and risen rapidly during culture Up to threshold value, then stable pH value and temperature culture, are co-cultured 5 days;The strain that lives is moved in second blake bottle respectively and added again Add nutrient solution, nutrient solution PH is adjusted to 5-6, temperature is 0 to 10 degree, and PH is heightened rapidly to threshold value, temperature during culture Degree is quickly raised to threshold value, then stable pH value and temperature culture, co-cultures 2 days;The strain that lives is moved to the 3rd culture respectively New nutrient solution is added again in bottle, nutrient solution PH is adjusted to 6-7, temperature is 10 to 20 degree, and PH is rapid during culture Heighten to threshold value, temperature is quickly raised to threshold value, then stable pH value and temperature culture, co-cultures 3 days;The strain that will live moves respectively New nutrient solution is added again into the 4th blake bottle, nutrient solution PH is adjusted to 7-8, temperature is 20-30 degree, in the mistake of culture PH is heightened rapidly to threshold value in journey, and temperature is quickly raised to threshold value, then stable pH value and temperature culture, co-cultures 5 days;It will live Strain is moved in the 5th blake bottle and adds new nutrient solution again respectively, and nutrient solution PH is adjusted to 7-8, and temperature is 30-40 degree, PH is heightened rapidly to threshold value during culture, and temperature is quickly raised to threshold value, then stable pH value and temperature culture, co-cultures 6 days, each flora number is adjusted to following scope after purifies and separates:The flora number of saccharomycete is 10 × 106-15×106CFU/g, bud The flora number of spore bacillus is 8 × 106-10×106CFU/g, the flora number of streptomycete is 6 × 106-12×106CFU/g, nitrogen-fixing bacteria Flora number be 12 × 106-15×106CFU/g, the flora number of lactic acid bacteria is 8 × 106-9×106CFU/g, obtain the yeast Bacterium, bacillus, streptomycete, nitrogen-fixing bacteria, lactic acid bacteria;Wherein, PH heightens rapidly rapid to threshold value, temperature as PH is adjusted immediately Heighten and heightened for hour in 1 to threshold value.
The preparation method of the described long-acting organic biofertilizer using bean pulp fermentation, comprises the following steps:
Step 1: by dregs of beans, mushroom dreg, soy sauce waste liquid, seawood meal, shrimp shell meal, oyster shell whiting, attapulgite, tea seed cake, wood High-speed stirred 1 hour after the water of potato vinasse, corncob and 3 times of volumes is mixed, at the uniform velocity it is by irradiation field, irradiation dose 45KGy, exposure time are 15 seconds, obtain mixture A;
Step 2: mixture A is well mixed after fermentation 3-5 days with strain, by being dried to obtain fermentation material, by fermentation material 100 mesh sieve, which are crushed, after being mixed with Cortex Meliae extract, chelating amino acids agent obtains powder;
Step 3: the chitosan, starch, sesbania gum, sodium alginate are added into isometric water, after being heated to dissolving, Obtain mixing liquid B;
Step 4: mixing liquid B is mixed with the powder that step 2 obtains, after stirring, fertiliser granulates are made, dry Obtain the long-acting organic biofertilizer using bean pulp fermentation.
Comparative example 1, same as Example 1, difference is that strain such as changes at the saccharomycete of parts by weight.
Comparative example 2, same as Example 1, difference is that strain such as changes at the bacillus of parts by weight.
Comparative example 3, same as Example 1, difference is that strain such as changes at saccharomycete and the streptomycete of parts by weight, wherein heavy Score is measured with also same as Example 1.
Comparative example 4, same as Example 1, difference is that strain such as changes at saccharomycete, bacillus and the strepto- of parts by weight Bacterium, wherein weight score are with also same as Example 1.
Comparative example 5, same as Example 1, difference is that strain such as changes at the bacillus and lactic acid bacteria of parts by weight, wherein Weight score is with also same as Example 1.
Experiment 1:Experiment in cultivation is done to dragon fruit using embodiment 1-3 and comparative example 1-5, chooses 900 plants of dragon fruit seedlings, with Machine is divided into 9 groups, every group of 100 young plants, is planted in respectively in 9 blocks of soil, equirotal planting site, 100 plants of fire of every piece of ground plantation Long Guomiao, respectively A, B, C, D, E, F, G, H, I group, A, B, C, D, E, F, G, H, I be respectively it is corresponding spray embodiment 1-3 with it is right Ratio 1-5 fertilizer and blank group, different amounts is sprayed according to growth each stage of dragon fruit, wherein in same stage, together When spray identical embodiment 1-3 and comparative example 1-5 fertilizer, Seedling Stage applied fertilizer every plant of 50g, growth stage every 15 days every 10 days Apply fertilizer every plant of 200g, yield positive results the phase every 15 days apply fertilizer every plant of 200g, other plantation method it is identical, record first plant of flue The average insect pest number of fruit Post flowering result maturation time, every plant of yield of the first annual and every plant, such as following table:
Table 1
Embodiment Packet Maturation time Single plant yield Average pest and disease damage number
Embodiment 1 A 9.5 months 32kg 1
Embodiment 2 B 8.5 months 35kg 0
Embodiment 3 C 8.5 months 36.8kg 0
Comparative example 1 D 16 months 24kg 6
Comparative example 2 E 15 months 23.5kg 5
Comparative example 3 F 14.5 individual month 24.5kg 6
Comparative example 4 H 14 months 25.2kg 7
Comparative example 5 I 12.5 individual month 26.4kg 5
Blank Sky is certainly 22 months 10kg 12
Significantly shortened using fertilizer dragon fruit growth cycle of the present invention by table 1 is visible, yield significantly improves, pest and disease damage number Significantly reduce.
Although embodiment of the present invention is disclosed as above, it is not restricted in specification and embodiment listed With it can be applied to various suitable the field of the invention completely, can be easily for those skilled in the art Other modification is realized, therefore under the universal limited without departing substantially from claim and equivalency range, it is of the invention and unlimited In specific details and shown here as the embodiment with description.

Claims (4)

1. a kind of long-acting organic biofertilizer using bean pulp fermentation, it is characterised in that described to have using the long-acting of bean pulp fermentation Machine bio-bacterial manure is mainly made up of following parts by weight material:Dregs of beans 50-100 parts, mushroom dreg 30-50 parts, soy sauce waste liquid 30-50 Part, seawood meal 8-12 parts, shrimp shell meal 8-15 parts, oyster shell whiting 10-15 parts, attapulgite 20-30 parts, tea seed cake 7-10 parts, cassiri Poor 20-30 parts, corncob 20-30 parts, Cortex Meliae extract 5-8 parts, chelating amino acids agent 1-5 parts, strain 1-5 parts, chitosan 5-8 parts, starch 50-100 parts, sesbania gum 5-10 parts, sodium alginate 20-25 parts;Wherein, the strain consists of:Yeast Bacterium, bacillus, streptomycete, nitrogen-fixing bacteria, lactic acid bacteria, each strain are that 1: 1: 0.2: 0.5: 0.8 mixing forms according to weight ratio, ferment The flora number of female bacterium is 10 × 106-15×106CFU/g, the flora number of bacillus is 8 × 106-10×106CFU/g, strepto- The flora number of bacterium is 6 × 106-12×106CFU/g, the flora number of nitrogen-fixing bacteria is 12 × 106-15×106CFU/g, lactic acid bacteria Flora number is 8 × 106-9×106CFU/g。
2. the long-acting organic biofertilizer according to claim 1 using bean pulp fermentation, it is characterised in that described to utilize beans The long-acting organic biofertilizer of dregs of rice fermentation is mainly made up of following parts by weight material:80 parts of dregs of beans, 35 parts of mushroom dreg, soy sauce give up 45 parts of liquid, 10 parts of seawood meal, 12 parts of shrimp shell meal, 12 parts of oyster shell whiting, 25 parts of attapulgite, 8 parts of tea seed cake, 25 parts of cassava grain stillage, jade 25 parts of core of rice, 7 parts of Cortex Meliae extract, 4 parts of chelating amino acids agent, 3 parts of strain, 7 parts of chitosan, 85 parts of starch, sesbania gum 8 Part, 23 parts of sodium alginate.
3. the long-acting organic biofertilizer according to claim 2 using bean pulp fermentation, it is characterised in that the yeast Bacterium, bacillus, streptomycete, nitrogen-fixing bacteria, lactic acid bacteria are tamed by following steps respectively:By saccharomycete, bacillus, strepto- Bacterium, nitrogen-fixing bacteria, lactic acid bacteria move to the first blake bottle addition nutrient solution, the preparation of nutrient solution respectively:Contain 20-50% mass fractions Sucrose, 1% mass fraction potassium chloride, 10% mass fraction gelatinized starch, surplus be water, nutrient solution PH is adjusted to 4-5, temperature Spend for -10 to 0 degree, cultivate 2-5 days;The strain that will live moves in second blake bottle respectively adds nutrient solution again, by nutrient solution PH is adjusted to 5-6, and temperature is 0 to 10 degree, is cultivated 2-5 days;The strain that will live moves in the 3rd blake bottle respectively to be added newly again Nutrient solution, nutrient solution PH is adjusted to 6-7, temperature is 10 to 20 degree, is cultivated 2-5 days;The strain that will live moves to the 4th respectively Blake bottle in add new nutrient solution again, nutrient solution PH is adjusted to 7-8, temperature is 20-30 degree, cultivate 2-5 days;It will live Strain is moved in the 5th blake bottle and adds new nutrient solution again respectively, and nutrient solution PH is adjusted to 7-8, and temperature is 30-40 degree, Cultivate 3-7 days, adjust each flora number to following scope after purifies and separates:The flora number of saccharomycete is 10 × 106-15× 106CFU/g, the flora number of bacillus is 8 × 106-10×106CFU/g, the flora number of streptomycete is 6 × 106-12× 106CFU/g, the flora number of nitrogen-fixing bacteria is 12 × 106-15×106CFU/g, the flora number of lactic acid bacteria is 8 × 106-9×106CFU/ G, obtain the saccharomycete, bacillus, streptomycete, nitrogen-fixing bacteria, lactic acid bacteria.
A kind of 4. preparation side of any described long-acting organic biofertilizer using bean pulp fermentation in 1-3 according to claim Method, it is characterised in that the preparation method comprises the following steps:
Step 1: by dregs of beans, mushroom dreg, soy sauce waste liquid, seawood meal, shrimp shell meal, oyster shell whiting, attapulgite, tea seed cake, cassiri High-speed stirred 1-2 hours after the water of grain, corncob and 2-3 times of volume is mixed, at the uniform velocity it is by irradiation field, irradiation dose 30-50KGy, exposure time are the 10-20 seconds, obtain mixture A;
Step 2: mixture A is well mixed after fermentation 3-5 days with strain, by being dried to obtain fermentation material, by fermentation material and hardship 100 mesh sieve, which are crushed, after chinaberry bark extract, chelating amino acids agent mixing obtains powder;
Step 3: the chitosan, starch, sesbania gum, sodium alginate are added into isometric water, after being heated to dissolving, obtain Mixing liquid B;
Step 4: mixing liquid B is mixed with the powder that step 2 obtains, after stirring, fertiliser granulates are made, are dried to obtain The long-acting organic biofertilizer using bean pulp fermentation.
CN201710549284.3A 2017-07-07 2017-07-07 It is a kind of to utilize long-acting organic biofertilizer of bean pulp fermentation and preparation method thereof Pending CN107337517A (en)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109438068A (en) * 2018-12-13 2019-03-08 中山市承铭农业技术开发有限公司 A method of Liquid Fertilizer is made using soy sauce factory waste liquid
CN109956781A (en) * 2017-12-25 2019-07-02 郴州市通源生物科技有限公司 A kind of organic fertilizer of high organic content and its preparation method and application

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CN101531548A (en) * 2008-12-11 2009-09-16 广东海洋大学 Method for preparing pre-vulcanized natural latex based slow release fertilizer
CN103819267A (en) * 2014-02-07 2014-05-28 湖南泰谷生物科技股份有限公司 Bacterial manure for peanut planting and preparation method of bacterial manure

Patent Citations (2)

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Publication number Priority date Publication date Assignee Title
CN101531548A (en) * 2008-12-11 2009-09-16 广东海洋大学 Method for preparing pre-vulcanized natural latex based slow release fertilizer
CN103819267A (en) * 2014-02-07 2014-05-28 湖南泰谷生物科技股份有限公司 Bacterial manure for peanut planting and preparation method of bacterial manure

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109956781A (en) * 2017-12-25 2019-07-02 郴州市通源生物科技有限公司 A kind of organic fertilizer of high organic content and its preparation method and application
CN109438068A (en) * 2018-12-13 2019-03-08 中山市承铭农业技术开发有限公司 A method of Liquid Fertilizer is made using soy sauce factory waste liquid

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Application publication date: 20171110