CN103409357A - Microbial agent and application for treating cultivating wastes - Google Patents
Microbial agent and application for treating cultivating wastes Download PDFInfo
- Publication number
- CN103409357A CN103409357A CN2013104086242A CN201310408624A CN103409357A CN 103409357 A CN103409357 A CN 103409357A CN 2013104086242 A CN2013104086242 A CN 2013104086242A CN 201310408624 A CN201310408624 A CN 201310408624A CN 103409357 A CN103409357 A CN 103409357A
- Authority
- CN
- China
- Prior art keywords
- organic fertilizer
- bacillus
- thiobacillus
- cow dung
- animal excrement
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/30—Fuel from waste, e.g. synthetic alcohol or diesel
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Abstract
The invention provides a live microbial composition for fermentation treatment of cultivating wastes. The composition is characterized by consisting of bean fiber monad, bacillus stearothermophilus, lactobacillus, methanobacteria, thiobacillus, bacillus licheniformis, bacillus polymyxa, streptomycete, thermoactinomycete, thermomonospora, nitrogen-fixing bacteria, nitrated monad, nitrobacteria, rhizobium, Aspergillus oryzae, yeasts, moulds, humicola, rhizopus and glomus mosseae. In addition, the invention further provides an organic fertilizer generated by virtue of fermentation of the microbial composition as well as an application and a method thereof. In production of the organic fertilizer, cultivating wastes such as animal wastes are objectively treated.
Description
Technical field
The present invention is dividing an application of No. 201210352489.Xth, Chinese patent, requires the applying date of enjoying this application.Due to the present invention to Genes For Plant Tolerance continuous cropping effect, anti-meet the improvement of the aspects such as batch effect and disease and insect resistance in, objectively dispose directlyly and beyond all doubtly and (resource utilization) breeding waste such as animal excrement.
Therefore, the invention belongs to the microbial fermentation technology field, particularly, the present invention relates to microbial composite, in the situation that substantially do not reduce the microbial inoculum Stability and adaptability by its generation, reduced bacterial classification quantity, reduced production cost, but the organic fertilizer that fermentation produces still can meet national standard fully, but also dispose, (resource utilization) breeding waste such as animal excrement, and to Genes For Plant Tolerance continuous cropping effect, anti-ly meet the aspects such as batch effect and disease and insect resistance and be improved.In addition, the invention still further relates to organic fertilizer and application and the method etc. that produce by the fermentation of mentioned microorganism composition.
Background technology
Current, along with the pay attention to day by day of people to healthy, food safety etc., more and more higher to the quality requirements of agricultural-food, wish that on the basis of agricultural-food increasing both production and income, the chemical fertilizer that can also eliminate agricultural-food is residual, is processed into real Organic food.For this reason, people have started to set about from the source that agricultural-food are cultivated, and the development organic fertilizer reduces or the use of replacing fertilizer fully.
For the fermentative production of organic fertilizer, its basis is that the composition of microbiobacterial agent has also played conclusive effect to its effect for the microbiobacterial agent of fermentation.The inventor's Chinese patent CN101643718 B and CN101629156 B disclose respectively the microbiobacterial agent as organic fertilizer and soil improvement agent, it adopts 25 kinds of microbial strainss to be composited, therefore when the raw material in the face of source difference, batch different organic fertilizer fermentation, can adapt to well the wherein existing differences such as carbon nitrogen content, pH value, inorganic salt, trace element, adapt to the required stability of large-scale commercial production, corresponding product can be increase soil fertility, and reduces harmful element content, eliminates pesticide residue and improvement saltings.But these prior aries are not studied whether to have anti-continuous cropping effect or resist and are met a batch effect, more there is no to enlighten the effect of the plant disease-resistant insect pest that can be improved.
Yet the inventor finds aborning, the microbial strains that crosses multiple types can bring the preparation flow complexity, the problem that preparation cost is high.But, multiple-microorganism has formed very complicated symbiotic relationship, the growing environment of different microorganisms requires also to have very big-difference, even wherein some bacterial classification can be secreted microbiotic, need other bacterial classifications to coordinate and produce collaborative tolerance, the destructing of these symbiotic relationships does not have the clearly instruction of prior art.So, rashly reduce the bacterial classification kind, can cause the unbalance of bacterial classification in whole microbial inoculum, for example, the inventor studies discovery, if remove cellulomonas cartae, it (may be to lack the growth microenvironment that cellulomonas cartae forms that the Bacterium lacticum in microbial inoculum, streptomycete and head mold also can't be survived substantially, also may lose the antibiotic ability of collaborative tolerance), the organic fertilizer that final fermentation produces can't be up to state standards; If remove the some mould, the microbial inoculum of production sometimes has a large amount of miscellaneous bacterias and produces, thereby suppresses the growth of the bacterium of adding in microbial inoculum, causes the quality of the microbial inoculum between each batch very unstable, thereby at all can't be as product promotion, and and so on and so forth.
For this reason, the inventor, through long-term arduous research, combines some fortune, if found, adopt the beans cellulomonas cartae (
Cellulomonasfabia) this specified strain, can in microbial inoculum, not add aspergillus niger and subtilis, thereby reduce production costs, but still can keep the stability of microbial inoculum to be suitable for suitability for industrialized production, and the organic fertilizer that makes fermentation produce can meet national standard fully, more surprisingly, by the product that this bacteria fermentation produces, not only can be increase soil fertility, also have anti-continuous cropping effect or anti-effect of meeting batch effect, more beyond thought is the characteristics that can also improve the plant disease-resistant insect pest, these characteristics make this tunning be particularly conducive to planting (namely continuously/in turn of soybean, continuous cropping/meet stubble to plant), and objectively dispose directlyly and beyond all doubtly and (resource utilization) breeding wastes such as animal excrement.
Summary of the invention
The object of the present invention is to provide microbial composite and/or microbial inoculum for the fermentative production organic fertilizer, in the situation that substantially do not reduce Stability and adaptability, reduced bacterial classification quantity, reduced production cost, but the organic fertilizer that fermentation produces still can meet national standard fully, but also brought Genes For Plant Tolerance continuous cropping effect, anti-improvement of meeting the aspects such as batch effect and disease and insect resistance, be specially adapted to the soybean planting industry.In addition, the present invention also aims to provide organic fertilizer and application and the method etc. that produce by mentioned microorganism composition and/or bacteria fermentation.
Particularly, in first aspect, the object of the present invention is to provide the microbial composite for the work of fermentative production organic fertilizer, it is characterized in that, described microbial composite by the beans cellulomonas cartae (
Cellulomonasfabia), bacstearothermophilus (
Bacillus sterothermophilus), Bacterium lacticum (
Lactobacillus), methagen (
Methanobacterium), thiobacillus (
Thiobacillus), Bacillus licheniformis (
Bacillus licheniformis), bacillus polymyxa (
Bacillus polymyxa), streptomycete (
Streptomyces), thermophilic actinomycete (
Thermoactinomyces), thermomonospora fusca (
Thermonospora), vinelandii (
Azotobacter), nitrated Zymomonas mobilis (
Nitrosomonas), bacterium nitrobacter (
Nitrobacter), root nodule bacterium (
Rhizobium), aspergillus oryzae (
Aspergillus oryzae), yeast (
Saccharomyces), mould (
Penicillium), humicola lanuginosa (
Humicola), head mold (
Rhizopus) and the VA Mycorrhizal Fungi composition.
Bacterial classification can be obtained from the nature separation by conventional strain identification separation method easily by those skilled in the art, but for the ease of the controlled fermentation quality of production, the bacterial classification preferably adopted is the type species of affiliated Pseudomonas or common bacterial classification, if passed through the bacterial classification of commercial channel open purchase, if bought by domestic and international spawn culture company, also can pass through biomaterial preservation center (as, ATCC of domestic CGMCC and CCTCC and the U.S. etc.) buys.Wherein, the bacterial classification of employing can be the bacterial classification in above-mentioned each Pseudomonas of mentioning, especially represents bacterial classification, and therefore, in a first aspect of the present invention, in described microbial composite, preferably Bacterium lacticum is common bacterial classification, most preferably be selected from lactobacterium casei (
Lactobacillus casei), it is particularly suited for the fermentation under slightly acidic pH environment;
The optimization methane bacillus is such as those disclosed in patent documentation US2008282733A, JP3250000A and US2008282733A etc., more preferably be selected from pselaphid alkane bacillus (
Methanobacterium formicium) and ruminate methagen (
Methanobacterium ruminantium) one or more, be most preferably pselaphid alkane bacillus (
Methanobacterium formicium) and ruminate methagen (
Methanobacterium ruminantium) mixture, it is particularly suited in neutrality to the fermentation under weakly alkaline pH environment;
Preferably thiobacillus is common bacterial classification, more preferably be selected from thiobacillus thiooxidans (
Thiobacillus thiooxidans) and thiobacillus ferrooxidant (
Thiobacillus ferrooxidans) one or more, be more preferably thiobacillus thiooxidans (
Thiobacillus thiooxidans) and thiobacillus ferrooxidant (
Thiobacillus ferrooxidans) mixture, it is particularly suited for the fermentation under the acid pH environment;
The preference chain mould is those disclosed in patent documentation US6399055B and JP3022921A, be most preferably streptomyces thermophilus (
Streptomyces thermophilus), can strengthen like this ability of microbiobacterial agent of the present invention at high temperature (55-65 ℃) bottom fermentation, it is particularly suited in neutrality to the fermentation under weakly alkaline pH environment;
Preferably thermophilic actinomycete is common bacterial classification, most preferably be selected from thermoactinomyces vulgaris (
Thermoactinomyces vulgaris), it is particularly suited for the fermentation under weakly alkaline pH environment;
Preferably thermomonospora fusca is those disclosed in patent documentations such as JP2108609A and JP59082085A, most preferably be selected from crooked thermomonospora fusca (
Thermonospora curvata), it is particularly suited for the fermentation under weakly alkaline pH environment;
Preferably vinelandii are common bacterial classifications, most preferably be selected from azotobacter vinelandii (
Azotobacter vinelandii), it is particularly suited for the fixed nitrogen under neutral pH environment;
Preferred nitrated Zymomonas mobilis is such as those disclosed in the patent documentations such as WO2007104268A, most preferably be selected from Nitrosomonas europaea (
Nitrosomonas europeae), it is particularly suited for from slightly acidic to the fixed nitrogen under weakly alkaline pH environment;
Preferably bacterium nitrobacter is such as those disclosed in the patent documentations such as CN101039692A, most preferably be selected from the Vickers bacterium nitrobacter (
Nitrobacter winogradskyi), it is particularly suited for from slightly acidic to the fixed nitrogen under weakly alkaline pH environment;
Preferably root nodule bacterium are common bacterial classifications, more preferably be selected from rihizobium japonicum (
Rhizobium japonicum) and rhizobium leguminosarum (
Rhizobium leguminosarum) one or more, be most preferably rihizobium japonicum (
Rhizobium japonicum) and rhizobium leguminosarum (
Rhizobium leguminosarum) mixture, it is particularly suited in neutrality to the fixed nitrogen under weakly acidic pH environment;
Preferred yeast is common bacterial classification, most preferably be selected from cereuisiae fermentum (
Saccharomyces cerevisiae), it is particularly suited for the fermentation under the acid pH environment;
Preferably mould is common bacterial classification, most preferably be selected from a mould (
Penicillium notatum), be particularly suited for like this fermentation under slightly acidic pH environment;
Preferably humicola lanuginosa is common bacterial classification, most preferably be selected from Humicola insolens (
Humicola insolens), it is particularly suited for from slightly acidic to the fermentation under neutral pH environment;
Preferably head mold is common bacterial classification, most preferably be selected from Rhizopus oligosporus (
Rhizopus oligosporus), it is particularly suited for the fermentation under slightly acidic pH environment;
And/or, preferred those disclosed in the Chinese patent literatures such as VA Mycorrhizal Fungi such as CN101144066A, CN 1307883C, more preferably be selected from rhizoctonia (
Rhizoctonia), most preferably be selected from Rhizoctonia cerealis (
Rhizoctonia cerealis), it is particularly suited for from slightly acidic to the fermentation under weakly alkaline pH environment.
Preferably in a first aspect of the present invention, described microbial composite by the beans cellulomonas cartae (
Cellulomonasfabia), bacstearothermophilus (
Bacillus sterothermophilus), lactobacterium casei (
Lactobacillus casei), pselaphid alkane bacillus (
Methanobacterium formicium), ruminate methagen (
Methanobacterium ruminantium), thiobacillus thiooxidans (
Thiobacillus thiooxidans), thiobacillus ferrooxidant (
Thiobacillus ferrooxidans), Bacillus licheniformis (
Bacillus licheniformis), bacillus polymyxa (
Bacillus polymyxa), streptomyces thermophilus (
Streptomyces thermophilus), thermoactinomyces vulgaris (
Thermoactinomyces vulgaris), crooked thermomonospora fusca (
Thermonospora curvata), azotobacter vinelandii (
Azotobacter vinelandii), Nitrosomonas europaea (
Nitrosomonas europeae), the Vickers bacterium nitrobacter (
Nitrobacter winogradskyi), rihizobium japonicum (
Rhizobium japonicum), rhizobium leguminosarum (
Rhizobium leguminosarum), aspergillus oryzae (
Aspergillus oryzae), cereuisiae fermentum (
Saccharomyces cerevisiae), the some mould (
Penicillium notatum), Humicola insolens (
Humicola insolens), Rhizopus oligosporus (
Rhizopus oligosporus) and Rhizoctonia cerealis (
Rhizoctonia cerealis) form.
In second aspect, the object of the present invention is to provide microbiobacterial agent, it is mixed and is obtained by the described microbial composite of first aspect present invention and matrix, and it comprises the described microbial composite of first aspect present invention.Wherein, it comprises the described microbial composite of first aspect, shows not and can make any bacterial classification inactivation in the described microbial composite of first aspect present invention due to antagonism and miscellaneous bacteria.Because wherein some bacterial classification can be secreted microbiotic, as streptomyces thermophilus and some mould, if there is no the microbiotic compacting, the miscellaneous bacteria of bringing from the stable matrix of difficult quality can be disturbed the stability of bacterial classification microbial inoculum, but in the time of in various bacterial classifications are mixed in a large number microbial inoculum matrix and finally are mixed in fermentation raw material, there is no observable microbiotic antagonistic effect, do not observe the unstable that miscellaneous bacteria is brought yet, this may be because in microbial inoculum preparation and fermenting process, microbiotic itself has collaborative tolerance to the antagonistic effect of other bacterial classifications between the limited or cooperation of bacterial classification, this is especially unforeseeable for reducing bacterial classification.In a word, in practice, all bacterial classifications have large number of viable in the microbial inoculum of preparation, and ratio is stable, can make fermenting process normally carry out.
Preferably in a second aspect of the present invention, the matrix that described microbiobacterial agent comprises can be that sterilising treatment is crossed, and can be also not pass through sterilising treatment.The main application of matrix is to make microorganic adhesion, is uniformly dispersed, so in matrix, vegetable fibre is often arranged in (normally plant fibrous waste); In order to make the bacterial classification in microbiobacterial agent can long-term surviving, in matrix, preferably also comprise the microbial nutrition composition, as animal excrement, animal-based protein, plant source protein and/or single cell protein etc.Common sponge has wood chip, rice husk, rice chaff, stalk etc.; Common animal excrement have human faecal mass, cow dung, pig manure, chicken manure etc.; Common animal-based protein has fish meal etc.; Common plant source protein has bean powder etc.; Single cell protein is generally used in feed, but when finding the preparing microorganism microbial inoculum, the inventor use single cell protein to help to improve the survival volume of bacterial classification, therefore in preferred substrate, contain single cell protein, common single cell protein such as Chinese patent application CN1426460A, CN1607908A etc. described those, can be by such as Norway Norferm DA, buying.Aspect preferred, described matrix comprises one or more of vegetable fibre, animal excrement, animal-based protein, plant source protein and single cell protein, more preferably described matrix comprises one or more of wood chip, rice husk, rice chaff, stalk, cow dung, pig manure, chicken manure, fish meal, bean powder and single cell protein, and most preferably described matrix is the mixture of rice husk, cow dung, fish meal, soyflour and single cell protein.
In addition, one independently aspect, the present invention also aims to be provided for the preparation method of the described microbiobacterial agent of second aspect present invention, it comprises the step that the described microbial composite of first aspect present invention is mixed with matrix.For handled easily, preferably with the bacterium liquid equal-volume of cultivating each bacterial classification, mix, also each mass mixing that becomes to grade in preferred substrate.After the step of preferably mixing, described preparation method also comprises the step that the mixture of gained is at room temperature placed.Like this, can make bacterial classification in matrix, breed and form stable ratio.In this article, room temperature has those skilled in the art's implication to understand, and for the sake of clarity, it refers to temperature between 15 and 30 degrees centigrade, preferably between 18 and 25 degrees centigrade, most preferably is 22 degrees centigrade.The time of usually placing is 2-10 week, until can form stable bacterial classification survival volume ratio, the time of placement is preferably 3-8 week, more preferably 4-7 week, most preferably is for 5 weeks.In placing, preferably to the mixture ventilation, can pass into gas by stirring, stirring, make the mixture ventilation if stirred weekly 1-5 time.
In the third aspect, the object of the present invention is to provide and have anti-continuous cropping effect and/or anti-organic fertilizer of meeting batch effect, it forms animal excrement and vegetable fibre fermentation by the described microbiobacterial agent of second aspect present invention.
In this article, term " continuous cropping " has the known meaning of agronomy those skilled in the art, refers on same plot continuous 2 years or several years plantation same crop.For example, on the same farmland, the First Year manufacturing soybean, the Second Year manufacturing soybean, the 3rd year manufacturing soybean, and the 4th year manufacturing soybean, after so wherein reaching in the 3rd year, the soybean of plantation all is called as secondary soybean.Continuous cropping can bring the continuous cropping effect, and directly index is exactly that the crop that its output reduces and crop quality is planted with respect to the main crop descends.The performance of continuous cropping effect in the plants such as soybean is particularly evident, distributes the handiness of plantation to bring restriction to farmland, easily produces " biennial bearing " phenomenon, by the demand growth belt, has carried out great difficulty to the peasant.
In this article, term " is met stubble " and is had the known meaning of agronomy those skilled in the art, refer on same piece of land, and a kind of crop of First Year plantation, Second Year is changed another kind of crop, the crop that the kind First Year was planted in the 3rd year.As, on the same farmland, the First Year manufacturing soybean, the Second Year maize planting, the 3rd year manufacturing soybean, so wherein the soybean of plantation in the 3rd year is for meeting a batch soybean.Meet stubble and can bring and meet a batch effect, directly to be exactly its output reduce with respect to the crop of the main crop plantation index.Meet batch performance of effect in the plants such as soybean also particularly evident, distribute the handiness of plantation to bring restriction to farmland, easily produce " biennial bearing " phenomenon, by the demand growth belt, carried out great difficulty to the peasant.
In this article, term " main crop " has the known meaning of agronomy those skilled in the art, refer on same piece of land, a kind of crop of plantation all do not plant in two years before this this kind crop.
When organic fertilizer of the present invention is used, can join the standard of using of existing machine fertilizer and use, for example can be used as base fertilizer and use, also can be used as topdresses uses.Amount of application can be determined according to the particular case in crop, farmland.
Preferably in a third aspect of the present invention, animal excrement are feces of livestock and poultry, are preferably selected from one or more of cow dung, pig manure, chicken manure, are most preferably cow dungs.In addition wherein, the preferred plant fiber is selected from one or more of wood chip, rice husk, rice chaff and stalk, is more preferably rice husk and/or stalk, is most preferably rice husk.
In fourth aspect, the object of the present invention is to provide the preparation method of the described organic fertilizer of third aspect present invention, it comprises the following steps successively:
(1) mix the described microbiobacterial agent of second aspect present invention, animal excrement and vegetable fibre;
(2) fermented mixture that step (1) is obtained, until be warmed up to 60-70 ℃, preferably be warmed up to 65 ℃;
(3) tunning that step (2) obtains is incubated at 60-70 ℃ of fermentation 5-18 days, the 7-15 days that preferably ferments, most preferably fermented 10 days;
(4) tunning that step (3) obtains is incubated at 45-55 ℃ of fermentation 7-20 days, preferably insulation, at 48 ~ 52 ℃, more preferably is incubated at 50 ℃; With
(5) the optional tunning that step (4) is obtained carries out high-temperature sterilization.
Due to the fermentation condition of common industrialized agriculture modifying agent, can't accomplish the clean level of food or industrial goods fermentation, constantly be subjected to the pollution of parasite and miscellaneous bacteria, in fermentation raw material, itself just exist simultaneously harmful parasite (as, roundworm egg) and disturb the miscellaneous bacteria of microbiobacterial agent growth of the present invention, the high temperature that therefore needs to utilize fermentation to produce is killed and suppresses.But, the inventor finds equally also can affect higher than the high temperature of 70 degrees centigrade growth, breeding and the fermentation of most of bacterial classifications in microbiobacterial agent of the present invention, even can thoroughly kill some bacterial classification in microbiobacterial agent of the present invention, fermentation is produced to extremely adverse influence.Therefore, the preparation method of industrialized agriculture modifying agent of the present invention should make killing parasite and suppress temperature in the step of miscellaneous bacteria breeding and should being controlled at below 70 ℃ of step (3).In the fermenting process of step (4), also should keep suitable high temperature (50 ℃ of left and right), the not the most suitable growth of most bacterial classifications, leavening temperature fermentation in microbiobacterial agent of the present invention, to avoid the impact of the too fast growth of miscellaneous bacteria under low temperature.For step (2) wherein, preferably insulation is at 65 ℃; Its heating-up time is generally 2-3 days.For step (4) wherein, the 10-15 days that preferably ferments, most preferably fermented 12 days.In the preparation method of industrialized agriculture modifying agent of the present invention, controlling temperature can only need by open frequency and the speed of stirring, stir mixture in fermenter, and the number of the extraneous air at room temperature passed into by control, just can control preferably automatization control.
Preferably in the step (5) of fourth aspect, the tunning step (4) not obtained carries out high-temperature sterilization.Yet, the inventor finds, for the stubble of meeting of soybean, plants at least, and sterilizing organic fertilizer of the present invention also can produce the advantageous effects significantly promoted with respect to contrast, therefore can be in the step (5) of fourth aspect, the tunning that step (4) is obtained carries out high-temperature sterilization.
Aspect the 5th, the object of the present invention is to provide the application of the described microbial composite of first aspect present invention in preparation has anti-continuous cropping effect and/or resists the organic fertilizer of meeting batch effect, preferably wherein organic fertilizer is the described organic fertilizer of third aspect present invention.
Aspect the 6th, the object of the present invention is to provide the application of the described microbiobacterial agent of second aspect present invention in preparation has anti-continuous cropping effect and/or resists the organic fertilizer of meeting batch effect, preferably wherein organic fertilizer is the described organic fertilizer of third aspect present invention.
Aspect the 7th, the object of the present invention is to provide the described organic fertilizer of third aspect present invention in crop-planting for anti-continuous cropping effect and/or anti-application of meeting batch effect.
In eight aspect, the object of the present invention is to provide the described microbial composite of first aspect present invention in preparation, to have the application of the organic fertilizer of disease and insect resistance, preferably wherein organic fertilizer is the described organic fertilizer of third aspect present invention, and more preferably it has anti-continuous cropping effect, anti-effect of meeting batch effect and disease and insect resistance simultaneously.
Aspect the 9th, the object of the present invention is to provide the described microbiobacterial agent of this second aspect in preparation, to have the application of the organic fertilizer of disease and insect resistance, preferably wherein organic fertilizer is the described organic fertilizer of third aspect present invention, and more preferably it has anti-continuous cropping effect, anti-effect of meeting batch effect and disease and insect resistance simultaneously.
Aspect the tenth, the object of the present invention is to provide the application for disease and insect resistance in crop-planting of the described organic fertilizer of third aspect present invention.
In preferably aspect of the present invention the 8th, nine and/or ten, disease and pest is root rot or root maggot disease preferably, is more preferably root rot and root maggot sick (that is, can resist simultaneously these two kinds of disease and pests).
In preferably aspect of the present invention the 5th, six, seven, eight, nine and/or ten, organic fertilizer for crop be soybean.For example, above-mentioned application is to have in preparation the application that Chinese People's Anti-Japanese Military and Political College's beans continuous cropping effect and/or Chinese People's Anti-Japanese Military and Political College's beans are met the organic fertilizer of batch effect, for anti-continuous cropping effect and/or anti-application of meeting batch effect in soybean planting, in preparation, to have the application of the organic fertilizer of Chinese People's Anti-Japanese Military and Political College's beans disease and pest, or in soybean planting for the application of disease and insect resistance.
The excellent effect that the present invention obtains comprises, in microbial composite/microbial inoculum of the present invention, bacterial classification quantity reduces to some extent, has saved production cost; Microbial composite/microbial inoculum of the present invention is good to the adaptability of fermentation raw material, and it is stable that wide accommodation, bacterial classification still can keep, and fermented quality is stable; The method of fermentative production of the present invention is easy, and the required equipment cost is low, both has been easy to promote, and also is easy to automatic production; The organic fertilizer that the present invention prepares not only can fertilizing soil, but also brought anti-continuous cropping effect and the anti-effect of meeting batch effect to plant, being conducive to the peasant selects flexibly and plants when the large plant variety of annual requirement, increase the handiness that the field-crop plantation distributes, be conducive to the kind that the peasant planting added value is high; The organic fertilizer that the present invention prepares also has the effect of disease and insect resistance, therefore can reduce the use of agricultural chemicals.
For the ease of understanding, below will to the present invention, be described in detail by specific embodiment.It needs to be noted, specific examples is only in order to illustrate, does not form limitation of the scope of the invention.Obviously those of ordinary skill in the art can illustrate according to this paper, within the scope of the invention the present invention is made to various corrections and change, and these corrections and change are also included in scope of the present invention.In addition, the present invention has quoted open source literature, and these documents are also in order more clearly to describe the present invention, and their full text content is all included the present invention in and carried out reference, just look like they full text in specification sheets of the present invention repeated description excessively the same.
Embodiment
Below by specific embodiment, describe, wherein special material, the step described in detail is well known to the skilled person, as can be referring to microorganism books or laboratory manuals such as " Bergey ' s Manual of Determinative Bacteriology ".
The production of embodiment 1 microbiobacterial agent
The present embodiment takes the preparing microorganism microbial inoculum with following bacterial classification: (1) beans cellulomonas cartae (
Cellulomonasfabia), (2) bacstearothermophilus (
Bacillus sterothermophilus), (3) lactobacterium casei (
Lactobacillus casei), (4) pselaphid alkane bacillus (
Methanobacterium formicium), (5) ruminate methagen (
Methanobacterium ruminantium), (6) thiobacillus thiooxidans (
Thiobacillus thiooxidans), (7) thiobacillus ferrooxidant (
Thiobacillus ferrooxidans), (8) Bacillus licheniformis (
Bacillus licheniformis), (9) bacillus polymyxa (
Bacillus polymyxa), (10) streptomyces thermophilus (
Streptomyces thermophilus), (11) thermoactinomyces vulgaris (
Thermoactinomyces vulgaris), (12) crooked thermomonospora fusca (
Thermonospora curvata), (13) azotobacter vinelandii (
Azotobacter vinelandii), (14) Nitrosomonas europaea (
Nitrosomonas europeae), (15) Vickers bacterium nitrobacter (
Nitrobacter winogradskyi), (16) rihizobium japonicum (
Rhizobium japonicum), (17) rhizobium leguminosarum (
Rhizobium leguminosarum), (18) aspergillus oryzae (
Aspergillus oryzae), (19) cereuisiae fermentum (
Saccharomyces cerevisiae), (20) some mould (
Penicillium notatum), (21) Humicola insolens (
Humicola insolens), (22) Rhizopus oligosporus (
Rhizopus oligosporus), (23) Rhizoctonia cerealis (
Rhizoctonia cerealis).Above bacterial classification can be cultivated according to existing ordinary method, and for convenient for production, in the embodiment of the present invention, the nutrient solution of each single culture used is all purchased from grand celebration Bao Si biotechnology limited liability company.Respectively get the bacteria culture fluid mixing that 1L buys, form microbial composite, and mix with the fresh pig manure of 5kg rice husk, 5kg, 5kg fish meal, 5kg soyflour and 5kg single cell protein through drying treatment, under room temperature, placed for 5 weeks, stir weekly 3 times and make the mixture ventilation, then cryodrying is to water content
<20%, after pulverizing, namely obtain microbiobacterial agent, it is for fermentative production organic fertilizer after this.Get the microbiobacterial agent of different production batchs, each bacterial classification is wherein carried out to culture identification, the results are shown in Table 1.1, show that in microbiobacterial agent, every kind of bacterial classification all has survival, and the amount of survival of every kind of bacterial classification all reaches 10
6Individual/more than the gram microbial inoculum, although and batch between bacterium count stability and be worse than slightly that Chinese patent CN101643718 B reports, still, in stablizing controlled scope, still meet the standard of industrial applications.
The quantity of bacterial classification in table 1.1 microbiobacterial agent
The production of embodiment 2 organic fertilizer
Getting water content is the 65%(weight ratio) cow dung (moisture deficit can be added water) and after crushed the particle rice chaff that is less than 5mm with the mass ratio of 85:15, mix and pulverize, make in mixed cow dung and rice chaff the caking that does not have 40mm above.
Then, the mixture of 1 ton of above-mentioned cow dung and rice chaff is mixed with the microbiobacterial agent that 1kg embodiment 1 produces, pile in fermenter and form fertile heap.Fertile stack put during along with fermenting process, fertile heap can heat up naturally, this fermentation calefaction process needs 2-3 days usually, during can stir 1-2 time the fertilizer heap.Then, when the temperature of fertilizer heap rises to 65 ℃, open screening apparatus and stir fertile heap, the heat produced to distribute fermentation, guarantee that temperature is no more than 70 ℃; When temperature, during lower than 63 ℃, close screening apparatus, by fermentation, fertile heap is heated up, this ferment at constant temperature process continues 10 days.Then, increase speed and frequency that screening apparatus stirs, make the temperature of fertile heap remain on 48 ~ 52 ℃, this slaking fermenting process continues 12 days.The organic fertilizer obtained gives out slight ammonia odor, does not make people's unplessantness displeasure.
Finally, by the end product of above-mentioned fermentation successively by dehumidifier dehumidify, granulator granulation, make the organic fertilizer particle, wherein in dehumidifying and the process of granulation, temperature all is no more than 70 ℃.This organic fertilizer particle, through check, meets China Ministry of Agriculture standard of the microorganism manure (NY227-94 and NY884-2002) fully.
Embodiment 3 organic fertilizer are on meeting the impact of batch soybean
The present embodiment has been described the inventor and has been entrusted national soybean Engineering Technical Research Centre in the continuous soybean planting test that the Harbin, Heilongjiang Province Acheng District carries out, to meet a batch volume analysis in 2009.
1, test materials and design
1.1 test materials
Biological organic fertilizer: in order to the organic fertilizer of the identical method production of embodiment 2
Soybean varieties: black agriculture 48.
1.2 test design
The crop of front stubble plantation is corn, and the soybean of epicycle plantation is for meeting a batch soybean.
Test is provided with four processing altogether:
(1) process 1: mu is executed Western composite fertilizer 20kg+ biological organic fertilizer 50kg of the present invention and is once applied as base fertilizer.
(2) process 2: the biological organic fertilizer 50kg of the present invention that mu is executed the processing of Western composite fertilizer 20kg+ process high-temperature sterilization once applies as base fertilizer.
(3) process 3: mu is executed Western composite fertilizer 20kg+ fine sand 50kg and is once applied as base fertilizer.
(4) process 4: mu is executed Western composite fertilizer 20kg and is once applied as base fertilizer.
2, test result analysis
2.1 the impact of biological organic fertilizer of the present invention on the soybean growing way
The soybean of using biological organic fertilizer of the present invention has growth soon, and seedling is strong, the characteristics that growing way is good.By table 3.1, can be found out, no matter whether biological organic fertilizer of the present invention, wherein exist viable bacteria, and the growing way that can make to meet batch soybean is obviously better, improves plant height 4 ~ 5cm; Exist in the situation of viable bacteria than non-existent, although growing way is better, not obvious.
The impact of table 3.1 different treatment on soybeans they grow
2.2 the impact of biological organic fertilizer on soybean yields
Result is as shown in table 3.2, use biological organic fertilizer of the present invention and can significantly improve single plant yield and grain weight, use sterilizing biological organic fertilizer of the present invention, per mu yield increases by 14.7% than contrast, and there is the biological organic fertilizer of the present invention of viable bacteria in use, per mu yield increases by 23.1% than contrast, also is significantly higher than sterilizing biological organic fertilizer of the present invention, shows the obvious effect of increasing production of the biological organic fertilizer of the present invention that has viable bacteria.
The impact of table 3.2 different treatment on soybean yields
| Treatment group | Plant height (cm) | The strain pod | The strain grain | 100-grain weight (g) | Per mu yield (kg) | Stimulation ratio (%) |
| Process 1 | 90.4 | 161.0 | 133.3 | 20.8 | 208.1 | 23.1 |
| Process 2 | 88.9 | 157.1 | 126.3 | 19.4 | 193.6 | 14.7 |
| Process 3 | 84.3 | 138.9 | 111.0 | 18.5 | 173.8 | 2.9 |
| Process 4 | 84.5 | 136.9 | 111.7 | 18.6 | 168.9 | - |
The impact of embodiment 4 organic fertilizer on secondary soybean
The present embodiment has been described the inventor and has been entrusted the continuous cropping volume analysis in the continuous soybean planting test that the Harbin, Heilongjiang Province Acheng District carries out in 2009 of national soybean Engineering Technical Research Centre.
1, test materials and design
1.1 test materials
Biological organic fertilizer: in order to the organic fertilizer of the identical method production of embodiment 2
Soybean varieties: dragon selects No. 1.
1.3 test design
The soybean of epicycle plantation is the secondary soybean of plantation in continuous the 3rd year, wards off in addition 5 mu of plantation main crop soybean in contrast.
Test is provided with four processing altogether:
(1) process 1: continuous cropping, mu is executed biological organic fertilizer 50kg of the present invention.
(2) process 2: continuous cropping, mu are executed biological organic fertilizer 25kg of the present invention and two ammonium 6kg, urea 2.5kg, vitriolate of tartar 2.5kg.
(3) process 3: continuous cropping, mu is executed biological organic fertilizer 25kg of the present invention.
(4) contrast: the main crop, mu is executed two ammonium 12kg, urea 5kg, vitriolate of tartar 5kg.
2, test result analysis
2.1 bio-bacterial manure affects continuous cropping soybeans they grow situation
By on September 14th, 2011 simultaneously to the investigation of plant height, strain root situation and disease and pest situation, the result drawn is as shown in table 4.1 and 4.2, biological organic fertilizer of the present invention can reduce the disadvantageous effect of continuous cropping to soybean plant height and fresh weight effectively, especially in the time of with the coupling of existing composite fertilizer, have the collaborative effect strengthened, significantly be better than the independent use of existing composite fertilizer; Formation for root nodule and lateral root also has significant enhancing ability, thereby can strengthen fixed nitrogen and root system, and when itself and the coupling of existing composite fertilizer, formation to root nodule has the collaborative effect strengthened, but to lateral root, but may certain inhibition be arranged due to the use of existing composite fertilizer, but effect also is better than the use of simple composite fertilizer; Biological organic fertilizer of the present invention is disease and insect resistance effectively, comprises anti-root rot and root maggot disease, in the time of especially with the coupling of existing composite fertilizer, has the collaborative ability that strengthens the disease and insect resistance effect.
The impact of table 4.1 different treatment on the soybean growing way
| Treatment group | Plant height (cm) | Strain fresh weight (g/ strain) | Root fresh weight (g/ strain) | 5 strain root nodule numbers (individual) | 5 strain root nodules heavy (g) | Strain lateral root number (individual) |
| Process 1 | 80.8 | 85.5 | 10.0 | 204 | 1.0 | 21.4 |
| Process 2 | 83.6 | 120.6 | 10.6 | 220 | 1.1 | 22.0 |
| Process 3 | 79.8 | 80.0 | 7.0 | 147 | 0.5 | 22.4 |
| Contrast | 79.3 | 95.5 | 9.0 | 184 | 0.8 | 19.6 |
The situation of soybean root disease and pest under table 4.2 different treatment
| Treatment group | Root rot sickness rate (%) | Root rot disease index (%) | Root maggot diseased plant rate (%) | Individual plant cyst roundworm number (individual) |
| Process 1 | 6 | 40.0 | 14.3 | 6 |
| Process 2 | 1 | 20.0 | 9.1 | 4 |
| Process 3 | 13 | 49.1 | 30 | 6 |
| Contrast | 8 | 46.7 | 25.9 | 12 |
2.2 the impact of bio-bacterial manure on soybean yields
The survey of output is produced the interpretation of result result as shown in table 4.3, biological organic fertilizer of the present invention does not have significant enhancement to soybean qualities such as soybean 100-grain weights, but with existing meet fertile coupling in, secondary soybean can be processed with respect to the main crop soybean yield-increasing 3.31% of conventional fertilizer application; Although use separately biological organic fertilizer of the present invention, secondary soybean output is with respect to the decline to some extent of the main crop soybean of conventional fertilizer application, but, with respect to approximately 19% output (according to local historical test data) that the secondary soybean of conventional fertilizer application descends, there is in addition the output of highly significant to promote.So, use biological organic fertilizer of the present invention and can effectively resist the continuous cropping effect.
The impact of table 4.3 different treatment on soybean yields
| Treatment group | 100-grain weight (g) | Per mu yield (kg) | Stimulation ratio (%) |
| Process 1 | 15.77 | 153.46 | -1.09 |
| Process 2 | 15.83 | 160.27 | 3.31 |
| Process 3 | 15.73 | 146.13 | -6.16 |
| Contrast | 15.83 | 155.13 | - |
Claims (10)
1. (preferably animal excrement, as cow dung for the fermentative processing breeding waste, pig manure, the microbial composite of work one or more of chicken manure), is characterized in that, described microbial composite is by beans cellulomonas cartae (Cellulomonas fabia), bacstearothermophilus (Bacillus sterothermophilus), Bacterium lacticum (Lactobacillus), methagen (Methanobacterium), thiobacillus (Thiobacillus), Bacillus licheniformis (Bacillus licheniformis), bacillus polymyxa (Bacillus polymyxa), streptomycete (Streptomyces), thermophilic actinomycete (Thermoactinomyces), thermomonospora fusca (Thermonospora), vinelandii (Azotobacter), nitrated Zymomonas mobilis (Nitrosomonas), bacterium nitrobacter (Nitrobacter), root nodule bacterium (Rhizobium), aspergillus oryzae (Aspergillus oryzae), yeast (Saccharomyces), mould (Penicillium), humicola lanuginosa (Humicola), head mold (Rhizopus) and VA Mycorrhizal Fungi form,
Preferably in described microbial composite, Bacterium lacticum is selected from lactobacterium casei (Lactobacillus casei); Methagen is selected from pselaphid alkane bacillus (Methanobacterium formicium) and ruminates one or more of methagen (Methanobacterium ruminantium); Thiobacillus is selected from one or more of thiobacillus thiooxidans (Thiobacillus thiooxidans) and thiobacillus ferrooxidant (Thiobacillus ferrooxidans); Streptomycete is selected from streptomyces thermophilus (Streptomyces thermophilus); Thermophilic actinomycete is selected from thermoactinomyces vulgaris (Thermoactinomyces vulgaris); Thermomonospora fusca is selected from crooked thermomonospora fusca (Thermonospora curvata); Vinelandii are selected from azotobacter vinelandii (Azotobacter vinelandii); Nitrated Zymomonas mobilis is selected from Nitrosomonas europaea (Nitrosomonas europeae); Bacterium nitrobacter is selected from Vickers bacterium nitrobacter (Nitrobacter winogradskyi); Root nodule bacterium are selected from one or more of rihizobium japonicum (Rhizobium japonicum) and rhizobium leguminosarum (Rhizobium leguminosarum); Yeast is selected from cereuisiae fermentum (Saccharomyces cerevisiae); Mould is selected from a mould (Penicillium notatum); Humicola lanuginosa is selected from Humicola insolens (Humicola insolens); Head mold is selected from Rhizopus oligosporus (Rhizopus oligosporus); And/or VA Mycorrhizal Fungi is selected from rhizoctonia (Rhizoctonia), more preferably be selected from Rhizoctonia cerealis (Rhizoctonia cerealis),
Most preferably described microbial composite is by beans cellulomonas cartae (Cellulomonas fabia), bacstearothermophilus (Bacillus sterothermophilus), lactobacterium casei (Lactobacillus casei), pselaphid alkane bacillus (Methanobacterium formicium), ruminate methagen (Methanobacterium ruminantium), thiobacillus thiooxidans (Thiobacillus thiooxidans), thiobacillus ferrooxidant (Thiobacillus ferrooxidans), Bacillus licheniformis (Bacillus licheniformis), bacillus polymyxa (Bacillus polymyxa), streptomyces thermophilus (Streptomyces thermophilus), thermoactinomyces vulgaris (Thermoactinomyces vulgaris), crooked thermomonospora fusca (Thermonospora curvata), azotobacter vinelandii (Azotobacter vinelandii), Nitrosomonas europaea (Nitrosomonas europeae), Vickers bacterium nitrobacter (Nitrobacter winogradskyi), rihizobium japonicum (Rhizobium japonicum), rhizobium leguminosarum (Rhizobium leguminosarum), aspergillus oryzae (Aspergillus oryzae), cereuisiae fermentum (Saccharomyces cerevisiae), point mould (Penicillium notatum), Humicola insolens (Humicola insolens), Rhizopus oligosporus (Rhizopus oligosporus) and Rhizoctonia cerealis (Rhizoctonia cerealis) form.
2. for (the preferably animal excrement of fermentative processing breeding waste, as cow dung, pig manure, one or more of chicken manure) microbiobacterial agent, it is mixed and is obtained by microbial composite claimed in claim 1 and matrix, and it comprises microbial composite claimed in claim 1, preferred wherein said matrix comprises vegetable fibre, animal excrement, animal-based protein, one or more of plant source protein and single cell protein, more preferably described matrix comprises wood chip, rice husk, rice chaff, stalk, cow dung, pig manure, chicken manure, fish meal, one or more of bean powder and single cell protein, most preferably described matrix is rice husk, cow dung, fish meal, the mixture of soyflour and single cell protein.
3. microbiobacterial agent claimed in claim 2, wherein in microbial composite claimed in claim 1, the amount of survival of every kind of bacterial classification all reaches 10
6Individual/more than the gram microbial inoculum, beans cellulomonas cartae, bacstearothermophilus, lactobacterium casei, pselaphid alkane bacillus in preferred every gram microbial inoculum, the quantity of ruminating methagen, thiobacillus thiooxidans, thiobacillus ferrooxidant, Bacillus licheniformis, bacillus polymyxa, streptomyces thermophilus, thermoactinomyces vulgaris, crooked thermomonospora fusca, azotobacter vinelandii, Nitrosomonas europaea, Vickers bacterium nitrobacter, rihizobium japonicum, rhizobium leguminosarum, aspergillus oryzae, cereuisiae fermentum, some mould, Humicola insolens, Rhizopus oligosporus and Rhizoctonia cerealis are respectively 1.63 x 10
8~ 1.51 x 10
8, 1.30 x 10
8~ 1.41 x 10
8, 1.12 x 10
8~ 9.77 x 10
7, 1.05x 10
8~ 1.29 x 10
8, 1.22 x 10
8~ 1.36x 10
8, 1.72 x 10
8~ 1.90 x 10
8, 1.53 x 10
8~ 1.37 x 10
8, 3.88 x 10
8~ 3.67 x 10
8, 7.96 x 10
7~ 7.58 x 10
7, 8.65 x 10
7~ 8.31 x 10
7, 8.60 x 10
7~ 9.12 x 10
7, 1.37 x 10
8~ 1.05 x 10
8, 5.33 x 10
7~ 5.80 x 10
7, 7.52 x 10
7~ 7.29 x 10
7, 9.31 x 10
7~ 8.84 x 10
7, 1.23 x 10
8~ 1.71 x 10
8, 1.65 x 10
8~ 1.38 x 10
8, 9.34 x 10
6~ 1.02 x 10
7, 2.28 x 10
7~ 2.35 x 10
7, 1.86 x 10
7~ 1.57 x 10
7, 4.09 x 10
6~ 4.22 x 10
6, 6.65 x 10
6~ 6.82 x 10
6With 1.68 x 10
7~ 1.84 x 10
7.
4. have anti-continuous cropping effect and/or anti-organic fertilizer of meeting batch effect, it forms animal excrement and vegetable fibre fermentation by microbiobacterial agent claimed in claim 2.
5. organic fertilizer claimed in claim 4, wherein animal excrement are feces of livestock and poultry, are preferably selected from one or more of cow dung, pig manure, chicken manure, are most preferably cow dungs; And/or vegetable fibre is selected from one or more of wood chip, rice husk, rice chaff and stalk, preferably rice husk.
6. the application of microbial composite claimed in claim 1 fermentative processing breeding waste (preferably animal excrement, as one or more of cow dung, pig manure, chicken manure) in preparing microbiobacterial agent.
7. the described microbiobacterial agent of claim 2 or 3 has the application of fermentative processing breeding waste in anti-continuous cropping effect and/or anti-organic fertilizer (the preferably described organic fertilizer of claim 4 or 5) of meeting batch effect (preferably animal excrement, as one or more of cow dung, pig manure, chicken manure) in preparation.
8. the described microbiobacterial agent of claim 2 or 3 has the application of fermentative processing breeding waste in the organic fertilizer (the preferably described organic fertilizer of claim 4 or 5) of disease and insect resistance (especially root rot and/or root maggot disease) (preferably animal excrement, as one or more of cow dung, pig manure, chicken manure) in preparation.
9. the described application of claim 7 or 8, wherein organic fertilizer for crop be soybean.
10. microbial composite claimed in claim 1 or the described microbiobacterial agent of claim 2 or 3 are in the application of fermentative processing breeding waste (preferably animal excrement, as one or more of cow dung, pig manure, chicken manure).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310408624.2A CN103409357B (en) | 2012-09-21 | 2012-09-21 | Microbial agent and application for treating cultivating wastes |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310408624.2A CN103409357B (en) | 2012-09-21 | 2012-09-21 | Microbial agent and application for treating cultivating wastes |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201210352489XA Division CN102839126B (en) | 2012-09-21 | 2012-09-21 | Microbial agent and application of antigravity and cropping effects of microbial agent |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103409357A true CN103409357A (en) | 2013-11-27 |
| CN103409357B CN103409357B (en) | 2015-05-13 |
Family
ID=49602400
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310408624.2A Active CN103409357B (en) | 2012-09-21 | 2012-09-21 | Microbial agent and application for treating cultivating wastes |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103409357B (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103865860A (en) * | 2014-04-01 | 2014-06-18 | 江苏省农业科学院 | Ternary compound inoculant for fermentation bed for pigs |
| CN106047770A (en) * | 2016-08-08 | 2016-10-26 | 江苏春语生物科技有限公司 | Composite liquid microbial agent with long quality guarantee period and preparation method thereof |
| CN110272843A (en) * | 2019-07-01 | 2019-09-24 | 温州科技职业学院 | It is a kind of for handling the composite bacteria agent and method of piggery waste water |
| CN112481159A (en) * | 2020-11-27 | 2021-03-12 | 广东丽豪生物农业有限公司 | Microbial agent and application thereof |
| WO2021080404A1 (en) * | 2019-10-24 | 2021-04-29 | Кунсулу Закарья | Method of producing biological substrate |
| CN113088464A (en) * | 2021-03-26 | 2021-07-09 | 张掖兰标生物科技有限公司 | Efficient microbial compound microbial inoculum for decomposing and treating feces and preparation method thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101643718A (en) * | 2009-08-26 | 2010-02-10 | 宋彦耕 | Microbial inoculum and organic fertilizers fermented by same |
| CN101781569A (en) * | 2009-11-27 | 2010-07-21 | 山东多利德生物科技有限公司 | Soil improvement and anti continuous cropping multifunctional biological agent |
| CN101843260A (en) * | 2009-03-25 | 2010-09-29 | 中国科学院沈阳应用生态研究所 | Method for preparing compound bactericide for preventing and controlling soybean root rot |
| CN102839126B (en) * | 2012-09-21 | 2013-10-09 | 宋彦耕 | Microbial agent and application of antigravity and cropping effects of microbial agent |
-
2012
- 2012-09-21 CN CN201310408624.2A patent/CN103409357B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101843260A (en) * | 2009-03-25 | 2010-09-29 | 中国科学院沈阳应用生态研究所 | Method for preparing compound bactericide for preventing and controlling soybean root rot |
| CN101643718A (en) * | 2009-08-26 | 2010-02-10 | 宋彦耕 | Microbial inoculum and organic fertilizers fermented by same |
| CN101781569A (en) * | 2009-11-27 | 2010-07-21 | 山东多利德生物科技有限公司 | Soil improvement and anti continuous cropping multifunctional biological agent |
| CN102839126B (en) * | 2012-09-21 | 2013-10-09 | 宋彦耕 | Microbial agent and application of antigravity and cropping effects of microbial agent |
Non-Patent Citations (3)
| Title |
|---|
| 李春金 等: "大豆专用复合药肥对大豆重茬产量的影响", 《辽宁农业科学》 * |
| 许艳丽 等: "复配生防菌株防治大豆根腐病的研究", 《大豆科学》 * |
| 邹德勋 等: "抗生菌剂在缓解大豆重迎茬根际微生态障碍中的作用", 《大豆科学》 * |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103865860A (en) * | 2014-04-01 | 2014-06-18 | 江苏省农业科学院 | Ternary compound inoculant for fermentation bed for pigs |
| CN106047770A (en) * | 2016-08-08 | 2016-10-26 | 江苏春语生物科技有限公司 | Composite liquid microbial agent with long quality guarantee period and preparation method thereof |
| CN110272843A (en) * | 2019-07-01 | 2019-09-24 | 温州科技职业学院 | It is a kind of for handling the composite bacteria agent and method of piggery waste water |
| WO2021080404A1 (en) * | 2019-10-24 | 2021-04-29 | Кунсулу Закарья | Method of producing biological substrate |
| CN112481159A (en) * | 2020-11-27 | 2021-03-12 | 广东丽豪生物农业有限公司 | Microbial agent and application thereof |
| CN112481159B (en) * | 2020-11-27 | 2023-04-11 | 广东丽豪生物农业有限公司 | Microbial agent and application thereof |
| CN113088464A (en) * | 2021-03-26 | 2021-07-09 | 张掖兰标生物科技有限公司 | Efficient microbial compound microbial inoculum for decomposing and treating feces and preparation method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103409357B (en) | 2015-05-13 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101643718B (en) | Microbial strain agent and organic fertilizers fermented by same | |
| CN101629156B (en) | Microbial agent and soil modifying agent produced by fermentation thereof | |
| CN104447038B (en) | A kind of biological organic inorganic composite fertiliser and its preparation method and application | |
| CN104560828B (en) | Microbial agent and application in prospect of passivating heavy metals of soil | |
| CN102229900B (en) | Compound probiotics preparation for biological organic fertilizers and preparation method thereof | |
| CN101659934B (en) | Antagonistic bacteria preventing and removing continuous cropping banana Panama wilt disease and microbial organic fertilizer thereof | |
| CN104560045B (en) | Microbial bacterial agent and chemical fertilizer excessively use growth-promoting secondary saline and alkaline and prone soil improved application | |
| CN102199049B (en) | Microbial organic manure and preparation method thereof | |
| CN104447095B (en) | A kind of biological activity organic medical fertilizer and its preparation method and application | |
| CN105218254A (en) | A kind of Microbial and ecological organic fertilizers material and preparation method thereof | |
| CN105296394A (en) | Microbial decomposition agent for animal wastes and straws and preparation method thereof | |
| CN103848698A (en) | Biological organic fertilizer prepared by aerobic fermentation of biogas residue and preparation method thereof | |
| CN102491802A (en) | Tea seed cake biological organic fertilizer and production method thereof | |
| CN104671860A (en) | Bioorganic fertilizer using agricultural waste as raw materials and preparation method of bioorganic fertilizer | |
| CN102827774B (en) | Microbial inoculum and application thereof in improvement of facility agriculture | |
| CN106083453A (en) | A kind of biomass organic fertilizer and preparation method thereof | |
| CN101717315B (en) | Special peanut organic and inorganic compound fertilizer for biologically preventing and treating root knot nematode disease and preparation method thereof | |
| CN103409357B (en) | Microbial agent and application for treating cultivating wastes | |
| CN106047764A (en) | Low-temperature beneficial microorganism preparation product applied to straw application | |
| CN109762765B (en) | Decomposed solid fermentation microbial inoculum and application thereof in agricultural wastes | |
| CN107646616A (en) | A kind of preparation method of floriculture substrate | |
| CN107445694A (en) | A kind of composite bio-chemical preparation and its applied in straw-returning | |
| CN108456021A (en) | A kind of biological organic fertilizer and preparation method thereof with decomposed stalk effect | |
| CN106045679A (en) | Preparation method of organic compound biological fertilizer special for peanuts | |
| CN103553831B (en) | A kind of agricultural biological fertilizer |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |