CN103865860A - Ternary compound inoculant for fermentation bed for pigs - Google Patents
Ternary compound inoculant for fermentation bed for pigs Download PDFInfo
- Publication number
- CN103865860A CN103865860A CN201410130166.5A CN201410130166A CN103865860A CN 103865860 A CN103865860 A CN 103865860A CN 201410130166 A CN201410130166 A CN 201410130166A CN 103865860 A CN103865860 A CN 103865860A
- Authority
- CN
- China
- Prior art keywords
- fungus agent
- composite fungus
- inoculant
- bedding
- padding
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Fodder In General (AREA)
Abstract
The invention provides a ternary compound inoculant for a fermentation bed for pigs. The ternary compound inoculant is prepared from a compound inoculant I, a compound inoculant II and a compound inoculant III, wherein the compound inoculant I is a starting inoculant and consists of trichoderma koningii, bacillus licheniformis and bacillus amyloliquefaciens; the compound inoculant II is a maintaining inoculant and consists of azotobacter chroococcum, enterococcus faecalis, bacillus laterosporus, bacillus amyloliquefaciens and bacillus subtilis; the compound inoculant III is a stacking inoculant and consists of aspergillus oryzae, streptomycete, azotobacter chroococcum, trichoderma koningii and bacillus licheniformis. The ternary compound inoculant provided by the invention is an efficient conversion flora system with complementary function and complementary enzyme system; the compound inoculant I is fermented to produce heat before entering; the compound inoculant II is subjected to biological deodorization, bacteria inhibition and waste transformation in cultivation; the compound inoculant III is decayed at high temperature after being discharged; nutrients are preserved in situ. The ternary compound inoculant system is collaboratively progressive, a continuous circulating fermentation process for cultivation of the fermentation bed is achieved, and healthy and clean production of pigs is facilitated.
Description
Technical field
The present invention relates to a boar fermentation bed tri compound microbial inoculum, belong to microbial engineering field.
Background technology
In recent years; along with the fast development of China's mass-producing, intensive industrialized piggery; production efficiency is greatly improved; and traditional cement flooring pattern of raising has also produced some drawbacks; the one, pig manure is concentrated discharge; need large-area middenstead and wash away water, wasting resource and labour, and causing the severe contamination of breeding environment and surrounding enviroment.
Fermentation bed to raise pig technology, based on controlling feces of livestock and poultry discharge and a kind of Novel cultivation mode of polluting, its ultimate principle is to realize the original position degraded of livestock and poultry fecaluria, improve greatly breeding environment, be an environmental protection agrotechnique, significant at the aspect such as livestock birds health cultivation and prevention and cure of pollution.
The process that fermentation bed to raise pig pad material fermentation decomposes fecaluria is the result of microbial process, so the height of fermented bacterium vigor has determined that ight soil decomposes and the efficiency of pad material fermentation, is the core link of fermenting bed of microorganism engineering technology.
At present, China applies the rise stage in fermentation bed, concentrate on the development of fermentation bed bacteria and the application in fermentation bed system thereof for the research of deep-litter systems, relating to more is single culture or the microorganism mix bacterium agent with degraded different substances ability, as a kind of by subtilis, plant lactobacillus, healthy and free from worry wood is mould, candida tropicalis forms starter in disclosed at patent documentation " a kind of high-efficiency compound microbial inoculum for fermenting beds and its production and use (CN200910180285.0) "; At patent documentation " a kind of microbial inoculum for high-efficiency fermenting bed and preparation method thereof (CN201110166962.0) ", a kind of starter being made up of subtilis, Bacillus licheniformis, Candida utilis is proposed; Disclose a kind of by morel at patent documentation " biological environmental production pig-raising fermentation bacterial and preparation method thereof (CN201110044903.0) ", bacstearothermophilus, bacstearothermophilus, thermophilic sour bifidus bacillus, subtilis, A Nandeshi streptomycete, the fermenting agent of yeast saccharomyces cerevisiae composition.Existing invention microbial inoculum all uses a kind of composite fungus agent in fermentation bed cultivation process.But entering hurdle, fatten and deliver sterilization for sale along with pig, nutrient, porosity (oxygen level) in fermenting bed padding are dynamic changes, in the culture-cycle of five wheat harvesting periods of a batch, along with the excretion of pig manure urine, the C/N of bedding and padding drops to 30:1 than from 250:1, porosity drops to 48% by 75%, and the change of essence has occurred the physico-chemical property of bedding and padding.And bedding and padding are environment support of microbial physiology activity, the change of bedding and padding physico-chemical property directly has influence on beneficial microorganism vigor in bedding and padding, has also determined the kind of bedding and padding microorganisms.Therefore, according to the different times (enter hurdle, cultivate, deliver for sale) of cultivation, the types of spawn that compatibility is different, the complex micro organism fungicide of select safety, stable, degradation rate is high and function has a bacterial strain composition of complementary action is the favourable assurance of the clean cultivation of fermentation bed.
Summary of the invention
The design deficiency that the object of the invention is to overcome existing a kind of microbial inoculum of culture-cycle, provides a boar fermentation bed tri compound microbial inoculum.The present invention is in the different steps (enter hurdle, cultivate, deliver for sale) of cultivation, take three kinds of different complex microbial inoculum combinations, give full play to the synergy of each microorganism in microbial inoculum, reduce the loss of the nutritive substances such as functional microorganism and nitrogen, suppress pathogenic bacteria, improve the efficiency of pig manure original position degraded, ensure the healthy cleaner production of pig.
Pig fermentation bed tri compound microbial inoculum of the present invention, by composite fungus agent I (startup microbial inoculum), composite fungus agent II (safeguarding microbial inoculum) and composite fungus agent III (accumulation microbial inoculum) composition.
Composite fungus agent I (startup microbial inoculum) starts fermentation for the bedding and padding matrix that pig enters before hurdle for the first time, composite fungus agent II (safeguarding microbial inoculum) is entered behind hurdle 1-2 month for pig, the routine maintenance of bedding and padding management phase, improve pig manure original position degradation efficiency, composite fungus agent III (accumulation microbial inoculum) is delivered the high temperature stacking fermentation of rear bedding and padding for sale for pig, promote the degree of depth of bedding and padding matrix to become thoroughly decomposed.
Described composite fungus agent I, for starting microbial inoculum, consists of the following composition according to weight percent: healthy and free from worry wood is mould 30%, Bacillus licheniformis 30%, bacillus amyloliquefaciens 40%;
Described composite fungus agent II, for safeguarding microbial inoculum, consists of the following composition according to weight percent: blown-ball Azotobacter 20%, enterococcus faecalis 20%, bacillus laterosporus 15%, bacillus amyloliquefaciens 25%, subtilis 20%;
Described composite fungus agent III, for piling up microbial inoculum, consists of the following composition according to weight percent: aspergillus oryzae 15%, and streptomycete 20%, blown-ball Azotobacter 25%, healthy and free from worry wood is mould 20%, Bacillus licheniformis 20%.
The know-why of pig fermentation bed tri compound microbial inoculum of the present invention is as follows:
1) composite fungus agent I (startup microbial inoculum), consists of the following composition according to weight percent: healthy and free from worry wood is mould 30%, Bacillus licheniformis 30%, bacillus amyloliquefaciens 40%.This composite fungus agent is entering for the first time while adding when bedding and padding matrix before pig is laid.In the bedding and padding matrix of just laying, the C/N ratio of bedding and padding is at 250:1-400:1, and a large amount of carbons is contained in the inside, and the microorganism of interpolation is all active stronger C source transformed bacterias.The mould breeding of healthy and free from worry wood is rapid, often breaks out at short notice, growth, metabolism, has stronger cellulolytic ability, can be take bagasse, stalk, agricultural byproducts, factory's Mierocrystalline cellulose waste residue as the mould song of substrate production of cellulose.Bacillus licheniformis is the genus bacillus that xylogen is decomposed in a strain, strong stress resistance, and breeding is rapid, quick heating.Bacillus amyloliquefaciens produces proteolytic enzyme, amylase, can utilize rapidly the organic carbons such as albumen, fat, carbohydrate, starch, promotes at short notice the easily conversion of degradation product in matrix, accelerates the intensification of matrix bedding and padding.With these three kinds of excellent species selection optimum proportion composition complex micro organism fungicides, function stem extracellular proteinase, amylase, cellulase, decompose xylogen, make the organic carbon fast decoupled in bedding and padding, the nutrient substance such as carbohydrate, nitrogen class that each bacterial strain transforms, promotes mutually the fast breeding of other bacterial strains.Enzyme between bacterial strain system is complementary, and nutrition is provided mutually, collaborative when decomposing organic carbon, can start fast the fermentation of fermentation bed matrix, and kill harmful germ, plays the effect of high-temperature sterilization, and what ensure swinery turns group smoothly, reduce swinery turn group stress.
2) composite fungus agent II (safeguarding microbial inoculum), consists of the following composition according to weight percent: blown-ball Azotobacter 20%, enterococcus faecalis 20%, bacillus laterosporus 15%, bacillus amyloliquefaciens 25%, subtilis 20%.This composite fungus agent is after pig enters hurdle 1-2 month, is used in the matrix routine maintenance of fermentation bed feeding and management phase, improves pig manure original position degradation efficiency.Along with a large amount of excretions of pig manure urine, the organism that bedding and padding matrix contains a large amount of carbon containings, nitrogen and fecal microorganism.In the microbiobacterial agent adding, except there being active stronger carbon source transformed bacteria, also need to select germ to suppress the deodorizing bacterium of bacterium and fixed nitrogen.Blown-ball Azotobacter can utilize starch, N.F,USP MANNITOL, utilizes nitrate, ammonia and amino acid as nitrogenous source, containing can fixed member nitrogen in a kind of nitrogen-free agar of organic carbon source.Enterococcus faecalis amphimicrobian, lactic acid producing, Gu ammonia reduces stink, effectively suppresses harmful microbe activity, promotes organic fermentation to decompose.Bacillus laterosporus strong stress resistance, can resistance to 1%-7%NaCl concentration, can suppress the pathogenic bacterias such as streptococcus aureus.Bacillus amyloliquefaciens produces proteolytic enzyme, amylase, can utilize rapidly the organic carbons such as albumen, fat, carbohydrate, starch, promotes at short notice the organic conversion of ight soil, reduces the corruption of ight soil.Subtilis is the probiotics that fodder industry is conventional, due to gemma protection, and energy tolerate fermentable high temperature; growth vigor is strong; this strain bacterium, except enzymes such as secretion catalase, urase, proteolytic enzyme, can utilize pig manure ramp, can also suppress the pathogenic bacteria such as Salmonellas, suis.Select optimum proportion composition complex micro organism fungicide with these five kinds of excellent species, suppress bacterium (bacillus laterosporus, subtilis, enterococcus faecalis) containing organic transformed bacteria (bacillus amyloliquefaciens, subtilis), solid ammonia deodorizing bacterium (blown-ball Azotobacter, enterococcus faecalis) and germ.Bacillus laterosporus, subtilis, the restraining effect of enterococcus faecalis to pathogenic bacteria, reduced the corruption of ight soil, promotes bacillus amyloliquefaciens, the conversion of blown-ball Azotobacter to C, N element.Meanwhile, C, N transformed bacteria are also for the metabolism of cause of disease inhibition bacterium provides good nutrient environment.Each bacterial strain, in the multiple functions of performance self, acts synergistically, energy rapid conversion ight soil, and the solid ammonia of deodorizing, suppresses pathogenic bacteria, has promoted the healthy growth of swinery.On the other hand, existing microbial inoculum is mostly containing lignin degrading, cellulosic bacterial strain.In the routine maintenance of fermentation bed, should be to transform ight soil, solid ammonia deodorizing as production object.Can decompose bedding and padding matrix containing lignin degrading, cellulosic bacterial strain, affect bedding and padding working lives.Composite fungus agent II in the present invention safeguards that microbial inoculum does not add the fungus strain combination of degraded cellulose, xylogen, thereby degraded bedding and padding that can be not meaningless can make fermenting bed padding increase the service life.
3) composite fungus agent III (accumulation microbial inoculum), consists of the following composition according to weight percent: aspergillus oryzae 15%, streptomycete 20%, blown-ball Azotobacter 25%, healthy and free from worry wood are mould 20%, Bacillus licheniformis 20%.This composite fungus agent is the high temperature stacking fermentation of delivering rear bedding and padding for pig for sale.Through the feeding period of five months, the organism of pig manure is most of conversion, contains the Mierocrystalline cellulose and the xylogen that are difficult in a large number conversion in bedding and padding, N element bulk deposition, and the C/N of bedding and padding is than dropping to 30:1 from the 250:1 entering pig.Traditional cement is raised, swinery whole enter whole go out after, all can carry out not the swinery of next batch by chemical disinfection and enter hurdle and carry out epidemic preventing working.Chemical disinfection can suppress the activity of bedding and padding probiotics, is not suitable for the disinfectant anti-epidemic that fermentation bed is raised.Therefore, by high-temperature sterilization kill intestinal bacteria, parasite is an important process link after fermentation bed swinery is delivered for sale.The microbiobacterial agent adding, except there being the high temperature bacterium of becoming thoroughly decomposed, also will comprise vinelandii.Aspergillus oryzae has very high protease activity and cellulase activity, for fermented feed.Streptomycete is Gram positive actinomycetes, transforms nitrogen phosphorus, produces various antibiotics, can directly suppress pathogenic bacteria.Blown-ball Azotobacter can utilize starch, N.F,USP MANNITOL, utilizes nitrate, ammonia and amino acid as nitrogenous source.Containing can fixed member nitrogen in a kind of nitrogen-free agar of organic carbon source.The mould breeding of healthy and free from worry wood is rapid, often breaks out at short notice, growth, metabolism, has stronger cellulolytic ability, can be take bagasse, stalk, agricultural byproducts, factory's Mierocrystalline cellulose waste residue as the mould song of substrate production of cellulose.Bacillus licheniformis is the genus bacillus that xylogen is decomposed in a strain, strong stress resistance, and breeding is rapid, quick heating.With these five kinds of excellent species selection optimum proportion composition composite bacteria agents, wherein, healthy and free from worry wood is mould, aspergillus oryzae, Bacillus licheniformis energy fast degradation Mierocrystalline cellulose, xylogen, thereby make the intensification fermentation of fermentation bed matrix, kill harmful bacterium, plays the effect of high-temperature sterilization, promotes the degree of depth of pig manure to become thoroughly decomposed, streptomycete, blown-ball Azotobacter can reduce nitrogen, phosphorus loss in the process heating up simultaneously, increase bedding and padding matrix fertilizer efficiency.
The using method of pig fermentation bed tri compound microbial inoculum of the present invention:
1) composite fungus agent I new bedding and padding before entering pig for the first time use after entering bed, by 20-40 gram/m
2usage ratio add composite fungus agent I, after composite fungus agent I is dissolved with 35-40 ℃ of warm water, spray and add bedding and padding surface to, after fully mixing, start fermentation;
2) composite fungus agent II is used after swinery is raised 1-2 month, by 10-20 gram/m
2usage ratio add composite fungus agent II, after composite fungus agent II is dissolved with 35-40 ℃ of warm water, spray and add bedding and padding surface to, turn over rake and mix, for the bedding and padding routine maintenance of management phase, improve pig manure original position degradation efficiency;
3) composite fungus agent III is to use after swinery feeding period finishes to deliver for sale, by 25-50 gram/m
2usage ratio add composite fungus agent III, after composite fungus agent III is dissolved with 35-40 ℃ of warm water, be sprayed onto bedding and padding surface, after fully mixing, carry out high temperature stacking fermentation, promote the degree of depth of bedding and padding matrix to become thoroughly decomposed.
Compared with prior art, the invention has the beneficial effects as follows:
1), according to the pig production reality of cultivation phase, in conjunction with the physicochemical characteristics of bedding and padding, set up (enter hurdle, cultivate, deliver for sale) collaborative tri compound microbial inoculum going forward one by one, because of ground matching function bacterial classification aptly.
2) ternary microorganism microbial inoculum be all have complementary functions, enzyme is complementary Efficient Conversion flora system.Enter hurdle primary fermentation heat production; Biological deodorizing, inhibition germ, conversion ight soil in cultivation; Deliver rear high temperature for sale and become thoroughly decomposed, nutrient original position is saved from damage.Because the each component of microbial inoculum can be adjusted with the process of cultivation phase, reach and effectively utilized Microbial resources, improve the goal of the invention of pig manure original position degradation efficiency.
3) in cultivation routine maintenance, use composite fungus agent II, take ight soil conversion, inhibition germ as the composite bacterial strain of target, reduce the material conversion of inert base, extend the work-ing life of bedding and padding.
Accompanying drawing explanation
Fig. 1 is the temperature variation curve that starts the each test group of fermentation;
Fig. 2 is the temperature variation curve of the each test group of heap fermentation.
Embodiment
Following embodiment is intended to further illustrate the present invention, and unrestricted the present invention.
The 9 strain bacterium titles that the present invention adopts and bacterial classification source are as follows: healthy and free from worry wood mould (CICC40634), Bacillus licheniformis (CICC20085), bacillus amyloliquefaciens (CICC20164), blown-ball Azotobacter (CICC21685), enterococcus faecalis (CICC21869), bacillus laterosporus (CICC21185), subtilis (CICC21164), aspergillus oryzae (CICC40186) and streptomycete (CICC23624) are all purchased from CICC (Chinese industrial microbial strains preservation administrative center).
Embodiment 1 prepares tri compound microbial inoculum
Pig fermentation bed tri compound microbial inoculum of the present invention, is made up of composite fungus agent I, composite fungus agent II and composite fungus agent III.
Composite fungus agent I, for starting microbial inoculum, consists of the following composition according to weight percent: healthy and free from worry wood is mould 30%, Bacillus licheniformis 30%, bacillus amyloliquefaciens 40%;
Composite fungus agent II, for safeguarding microbial inoculum, consists of the following composition according to weight percent: blown-ball Azotobacter 20%, enterococcus faecalis 20%, bacillus laterosporus 15%, bacillus amyloliquefaciens 25%, subtilis 20%;
Composite fungus agent III, for piling up microbial inoculum, consists of the following composition according to weight percent: aspergillus oryzae 15%, and streptomycete 20%, blown-ball Azotobacter 25%, healthy and free from worry wood is mould 20%, Bacillus licheniformis 20%.
The preparation method of pig fermentation bed tri compound microbial inoculum of the present invention, comprises the steps:
The amplification of the configuration of every kind of composite fungus agent from slant strains to finished product will be passed through following three steps.
1) one-level spreads cultivation: the bacterial classification of each purified cultivation, after the inclined-plane of preservation takes out, is inoculated into respectively in the seed culture medium preparing and cultivates respectively, in the bottle of each 1L, fills substratum 300ml, is then placed in 36 ℃ of shaking culture 48h-72h on shaking table;
During one-level spreads cultivation, the slant medium of various bacterium and seed culture medium are known basic medium.Wherein, the seed culture medium of Bacillus licheniformis, bacillus amyloliquefaciens, bacillus laterosporus, subtilis---nutrient broth medium (culturing bacterium), healthy and free from worry wood is mould, aspergillus oryzae---PDA substratum (cultivating mould), enterococcus faecalis---MRS substratum (cultivating milk-acid bacteria), streptomycete---Gao Shi substratum (cultivating actinomycetes), blown-ball Azotobacter---No. 77 fixed nitrogen substratum (cultivating vinelandii).
2) secondary spreads cultivation: during detect respectively each bacteria culture fluid viable count, treat that each bacterium viable count reaches (1-6) × 10
9after/ml, take out seed liquor, make composite bacteria liquid.
The each bacterium seed liquor of each composite bacteria liquid composition and content as follows:
For the preparation of the composite bacteria liquid of composite fungus agent I---the mould seed liquor 120ml of healthy and free from worry wood, Bacillus licheniformis seed liquor 120ml, bacillus amyloliquefaciens seed liquor 160ml;
For the preparation of the composite bacteria liquid of composite fungus agent II---blown-ball Azotobacter seed liquor 80ml, enterococcus faecalis seed liquor 80ml, bacillus laterosporus seed liquor 60ml, bacillus amyloliquefaciens seed liquor 100ml, subtilis seed liquor 80ml;
For the preparation of the composite bacteria liquid of composite fungus agent III---aspergillus oryzae seed liquor 60ml, streptomycete seed liquid 80ml, blown-ball Azotobacter seed liquor 100ml, the mould seed liquor 80ml of healthy and free from worry wood, Bacillus licheniformis seed liquor 80ml.
Then be the ratio of composite bacteria liquid 10% and solid medium 90% according to weight ratio, the secondary that each composite bacteria liquid is inoculated in respectively separately spreads cultivation in substratum, mixes, and be jointly placed under 30-35 ℃ of culture condition and cultivate 72h-96h,
For the preparation of the secondary of the composite bacteria liquid of the composite fungus agent II substratum that spreads cultivation, every 1kg is grouped into by following one-tenth: wheat bran 200g, Semen Maydis powder 22g, dregs of beans 100g, glucose 20g, NaCl5g, potassium primary phosphate 3g, wood chip 200g, water 450g.
For the preparation of the secondary of the composite bacteria liquid of the composite fungus agent II substratum that spreads cultivation, every 1kg is grouped into by following one-tenth: dregs of beans 150g, Semen Maydis powder 62g, wheat bran 100g, glucose 30g, NaCl5g, potassium primary phosphate 3g, wood chip 200g, water 450g.
For the preparation of the secondary of the composite bacteria liquid of the composite fungus agent III substratum that spreads cultivation, every 1kg is grouped into by following one-tenth: wheat bran 160g, Semen Maydis powder 100g, glucose 35g, NaCl5g, wood chip 250g, water 450g.
3) solid material is dry: under 50-55 ℃ of condition, dry wet feed, can be made into composite fungus agent powder product, composite fungus agent I powder product bacteria containing amount is 2 × 10
9-4 × 10
9between cfu/g, composite fungus agent II powder product bacteria containing amount is 4 × 10
9-8 × 10
9cfu/g, composite fungus agent III powder product bacteria containing amount is 2 × 10
8-4 × 10
8cfu/g.
Embodiment 2 detects pig fermentation bed to raise pig effect that tri compound microbial inoculum of the present invention is made
Product fermentation bed cultivation technology pig-keeping experiment of the present invention has carried out on certain pig farm year October in July, 2013-2013.Bedding and padding are made up of by the weight ratio of 1: 1 saw dust, rice husk.By term, the principle that body weight is close, select same kind, with batch 60 of hybridized pigs, be divided at random 2 groups, 30 every group.Test I group is control group, traditional concrete floor hutch; Test II group, the pig fermentation bed cultivation made from tri compound microbial inoculum of the present invention.
Before entering pig, use composite fungus agent I (startup microbial inoculum), by 30 grams/m
2usage ratio add composite fungus agent I, after composite fungus agent I is dissolved with 35-40 ℃ of warm water 2L, consumption is by 30 grams/m
2ratio, spray add bedding and padding surface to, after fully mixing, start fermentation;
Enter pig after 45 days, using composite fungus agent II (safeguarding microbial inoculum), by 15 grams/m
2usage ratio add composite fungus agent II, after composite fungus agent II is dissolved with 35-40 ℃ of warm water 2L, spray and add bedding and padding surface to, turn over rake and mix, for the bedding and padding routine maintenance of management phase, improve pig manure original position degradation efficiency;
After finishing to deliver for sale, swinery feeding period uses composite fungus agent III (accumulation microbial inoculum), by 35 grams/m
2usage ratio add composite fungus agent III, after composite fungus agent III is dissolved with 35-40 ℃ of warm water 2L, be sprayed onto bedding and padding surface, after fully mixing, carry out high temperature stacking fermentation, promote the degree of depth of bedding and padding matrix to become thoroughly decomposed.
Control group is identical with test group basal diet.
Table 1 Performance of Finishing Pigs is measured
Table 2 fermentation bed and the comparison of traditional water punching circle growing and fattening pigs seroimmunity index
As shown in Table 1, from production performance, compared with effect that employing fermentation bed is raised and raise pigs in cement flooring, average weight gain and day weight gain improve respectively 17.69% and 17.71%, significant difference (P<0.05), feedstuff-meat ratio is with the obvious advantage, and table 2 shows that biological environmental production in this test (fermentation bed) pig house pig IgA, IgG, IgM concentration are higher than cement flooring pig house, but not remarkable (P > 0.05) of difference.Reach a conclusion thus, tri compound microbial inoculum of the present invention, has significantly improved day weight gain and the feed conversion rate of pig, for pig has been created good living environment, increase the immunizing power of pig, and has realized high yield, high-quality, the efficient target of raising pigs.
Embodiment 3 detects the bed body effect of tri compound microbial inoculum of the present invention in fermentation bed to raise pig is produced
The application experiment of product of the present invention in bed surface matrix carried out on certain pig farm year January in October, 2013-2014.
Test 1 group: commercially available external fermentation bed bacteria, containing subtilis, amylase, proteolytic enzyme, viable count 10
7cfu/g, additive capacity is 200g/m
3
Test 2 groups: sell domestic fermentation bed bacteria, containing milk-acid bacteria, sporeformer, yeast, viable count 10
9cfu/g, additive capacity is 20g/m
2
Test 3 groups: tri compound microbial inoculum of the present invention, entering before pig to use composite fungus agent I (startup microbial inoculum), by 30 grams/m
2ratio add, entering pig after 45 days, use composite fungus agent II (safeguarding microbial inoculum).By 15 grams/m
2ratio add.After pig is delivered for sale for 110 days, use composite fungus agent III (accumulation microbial inoculum), by 35 grams/m
2ratio be added to row heap fermentation.Respectively before production, produce in, produce after monitoring bedding and padding physico-chemical property.The temperature variation of bedding and padding matrix when monitoring starts fermentation, heap fermentation respectively.The nutrient content of measuring fermenting bed padding test group, result is shown in respectively Fig. 1, Fig. 2, table 3.
The nutrient content of table 3 fermenting bed padding
Fig. 1 is the temperature variation curve that starts fermentation.As shown in Figure 1, in the time starting fermentation, each test group is energy jigger mast material temperature degree all, and test group 3 can reach 52 ℃ at the 5th day, and temperature is the highest, intensification best results.
Fig. 2 is the temperature variation curve of heap fermentation.Compared with other two groups, test group 3, the highest heap temperature reaches between 50-55 ℃, continues 6 days, and the matrix of can high temperature becoming thoroughly decomposed, kills pathogenic bacteria.
As shown in Table 3, compared with test group 1,2, the ammonium nitrogen of test group 3, organic minimum, test group 2 is the highest, and test group 3 is than test group 2, and ammonia-nitrogen content has declined 33.23%, organic content has declined 9.59%, total N content, and test group 3 has improved 8.33% than test group 2.
Result shows, the effectively deodorizing of tri compound microbial inoculum, and degraded is organic, reduces the loss of N element, improves bedding and padding fertilizer efficiency.
Claims (2)
1. a boar fermentation bed tri compound microbial inoculum, is characterized in that, formed by composite fungus agent I, composite fungus agent II and composite fungus agent III,
Described composite fungus agent I, for starting microbial inoculum, consists of the following composition according to weight percent: healthy and free from worry wood is mould 30%, Bacillus licheniformis 30%, bacillus amyloliquefaciens 40%;
Described composite fungus agent II, for safeguarding microbial inoculum, consists of the following composition according to weight percent: blown-ball Azotobacter 20%, enterococcus faecalis 20%, bacillus laterosporus 15%, bacillus amyloliquefaciens 25%, subtilis 20%;
Described composite fungus agent III, for piling up microbial inoculum, consists of the following composition according to weight percent: aspergillus oryzae 15%, and streptomycete 20%, blown-ball Azotobacter 25%, healthy and free from worry wood is mould 20%, Bacillus licheniformis 20%.
2. the using method of pig fermentation bed tri compound microbial inoculum as claimed in claim 1, is characterized in that, comprises the steps:
1) composite fungus agent I new bedding and padding before entering pig for the first time use after entering bed, by 20-40 gram/m
2usage ratio add composite fungus agent I, after composite fungus agent I is dissolved with 35-40 ℃ of warm water, spray and add bedding and padding surface to, after fully mixing, start fermentation;
2) composite fungus agent II is used after swinery is raised 1-2 month, by 10-20 gram/m
2usage ratio add composite fungus agent II, after composite fungus agent II is dissolved with 35-40 ℃ of warm water, spray and add bedding and padding surface to, turn over rake and mix, for the bedding and padding routine maintenance of management phase, improve pig manure original position degradation efficiency;
3) composite fungus agent III is to use after swinery feeding period finishes to deliver for sale, by 25-50 gram/m
2usage ratio add composite fungus agent III, after composite fungus agent III is dissolved with 35-40 ℃ of warm water, be sprayed onto bedding and padding surface, after fully mixing, carry out high temperature stacking fermentation, promote the degree of depth of bedding and padding matrix to become thoroughly decomposed.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410130166.5A CN103865860B (en) | 2014-04-01 | 2014-04-01 | One boar fermentation bed tri compound microbial inoculum |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410130166.5A CN103865860B (en) | 2014-04-01 | 2014-04-01 | One boar fermentation bed tri compound microbial inoculum |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103865860A true CN103865860A (en) | 2014-06-18 |
| CN103865860B CN103865860B (en) | 2016-03-02 |
Family
ID=50904872
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410130166.5A Active CN103865860B (en) | 2014-04-01 | 2014-04-01 | One boar fermentation bed tri compound microbial inoculum |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103865860B (en) |
Cited By (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104498369A (en) * | 2014-12-05 | 2015-04-08 | 中国农业科学院植物保护研究所 | Trichoderma koningii and bacterial agent containing same and application thereof in prevention of cylindrocarpon destructans |
| CN104719170A (en) * | 2015-02-07 | 2015-06-24 | 江苏省农业科学院 | Pig house and method for resource utilization of straw as fermentation bed padding for pigs |
| CN105384480A (en) * | 2015-11-16 | 2016-03-09 | 北京嘉博文生物科技有限公司 | Biological humic acid organic material decomposition agent having deodorization function |
| CN105462878A (en) * | 2015-12-02 | 2016-04-06 | 江苏绿科生物技术有限公司 | Livestock excrement efficient decomposition agent and fermentation method thereof |
| CN106244481A (en) * | 2016-07-29 | 2016-12-21 | 河南普爱饲料股份有限公司 | A kind of compound micro-ecological preparation, its preparation method and application processed for farm animal excrement |
| CN106754571A (en) * | 2017-03-04 | 2017-05-31 | 江西顺泉生物科技有限公司 | A kind of complex microorganism deodorant and preparation method thereof |
| CN106754572A (en) * | 2017-03-04 | 2017-05-31 | 江西顺泉生物科技有限公司 | One bacillus amyloliquefaciens and application thereof |
| CN106905003A (en) * | 2017-04-12 | 2017-06-30 | 天津海泰市政绿化有限公司 | A kind of microbial inoculum combination of city grass cuttings fermentation and its zymotechnique |
| CN108144386A (en) * | 2018-02-05 | 2018-06-12 | 邱榕生 | A kind of method of poultry house air multistage cleaning deodorization |
| CN108211692A (en) * | 2017-12-29 | 2018-06-29 | 广州广牧丰生物技术有限公司 | A kind of biological deodorant and its application |
| CN109136127A (en) * | 2018-08-22 | 2019-01-04 | 宝蓝环保科技霸州有限公司 | A kind of kitchen garbage fermentation deodorization compound bacteria and preparation method thereof |
| CN109809659A (en) * | 2019-02-22 | 2019-05-28 | 武汉益锦祥生物环保有限公司 | A kind of microbial deoderizer and its preparation method and application |
| CN110024701A (en) * | 2019-04-23 | 2019-07-19 | 周宁县生隆科技养殖有限公司 | A kind of ecologically raising pigs fermentation bed and its management method |
| CN110038874A (en) * | 2019-04-04 | 2019-07-23 | 广西大自然再生资源有限公司 | Categorized consumer waste processing method |
| CN110129233A (en) * | 2019-03-15 | 2019-08-16 | 江苏省农业科学院 | A kind of microbial bacterial agent and preparation method thereof being used to prepare cage bird fermenting bed padding |
| CN110143835A (en) * | 2019-06-10 | 2019-08-20 | 刘坚 | A method of bio-fertilizer is prepared using discarded pig fermenting bed padding |
| CN113215055A (en) * | 2021-05-31 | 2021-08-06 | 湖北金地天成农业发展有限公司 | Organic fertilizer fermentation inoculant and preparation process thereof |
| WO2022104772A1 (en) * | 2020-11-23 | 2022-05-27 | 南京溧水高新创业投资管理有限公司 | Cyanobacteria composting composite microbial agent and preparation method therefor |
| CN117229938A (en) * | 2023-05-24 | 2023-12-15 | 贵州煜宏生物科技有限公司 | Fermentation bed microbial inoculum suitable for Zhenfeng Guizhou and preparation method |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101186879A (en) * | 2007-12-05 | 2008-05-28 | 中国科学院南京土壤研究所 | Agriculture castoff compost ternary microorganism composite microbial inoculum |
| CN101948763A (en) * | 2009-10-13 | 2011-01-19 | 中国农业科学院饲料研究所 | High-efficiency compound microbial inoculum for fermenting beds, and preparation method and application thereof |
| CN102286376A (en) * | 2011-06-21 | 2011-12-21 | 湖南省微生物研究所 | Microbial inoculum for high-efficiency fermenting bed and preparation method thereof |
| CN103409357A (en) * | 2012-09-21 | 2013-11-27 | 宋彦耕 | Microbial agent and application for treating cultivating wastes |
-
2014
- 2014-04-01 CN CN201410130166.5A patent/CN103865860B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101186879A (en) * | 2007-12-05 | 2008-05-28 | 中国科学院南京土壤研究所 | Agriculture castoff compost ternary microorganism composite microbial inoculum |
| CN101948763A (en) * | 2009-10-13 | 2011-01-19 | 中国农业科学院饲料研究所 | High-efficiency compound microbial inoculum for fermenting beds, and preparation method and application thereof |
| CN102286376A (en) * | 2011-06-21 | 2011-12-21 | 湖南省微生物研究所 | Microbial inoculum for high-efficiency fermenting bed and preparation method thereof |
| CN103409357A (en) * | 2012-09-21 | 2013-11-27 | 宋彦耕 | Microbial agent and application for treating cultivating wastes |
Non-Patent Citations (2)
| Title |
|---|
| 秦竹等: "发酵床生猪养殖中菌种与垫料的研究进展", 《安徽农业科学》, vol. 40, no. 30, 31 December 2012 (2012-12-31) * |
| 蒋建明等: "微生物发酵床养猪模式的关键技术研究与应用", 《江苏农业科学》, vol. 41, no. 9, 31 December 2013 (2013-12-31) * |
Cited By (25)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104498369A (en) * | 2014-12-05 | 2015-04-08 | 中国农业科学院植物保护研究所 | Trichoderma koningii and bacterial agent containing same and application thereof in prevention of cylindrocarpon destructans |
| CN104719170A (en) * | 2015-02-07 | 2015-06-24 | 江苏省农业科学院 | Pig house and method for resource utilization of straw as fermentation bed padding for pigs |
| CN105384480A (en) * | 2015-11-16 | 2016-03-09 | 北京嘉博文生物科技有限公司 | Biological humic acid organic material decomposition agent having deodorization function |
| CN105384480B (en) * | 2015-11-16 | 2019-05-28 | 北京嘉博文生物科技有限公司 | A kind of biological humic type organic matter decomposing inoculant with deodorization functions |
| CN105462878B (en) * | 2015-12-02 | 2019-01-25 | 江苏绿科生物技术有限公司 | A kind of efficient decomposing agent of feces of livestock and poultry and its fermentation process |
| CN105462878A (en) * | 2015-12-02 | 2016-04-06 | 江苏绿科生物技术有限公司 | Livestock excrement efficient decomposition agent and fermentation method thereof |
| CN106244481A (en) * | 2016-07-29 | 2016-12-21 | 河南普爱饲料股份有限公司 | A kind of compound micro-ecological preparation, its preparation method and application processed for farm animal excrement |
| CN106754572A (en) * | 2017-03-04 | 2017-05-31 | 江西顺泉生物科技有限公司 | One bacillus amyloliquefaciens and application thereof |
| CN106754571B (en) * | 2017-03-04 | 2019-06-21 | 江西顺泉生物科技有限公司 | A kind of complex microorganism deodorant and preparation method thereof |
| CN106754571A (en) * | 2017-03-04 | 2017-05-31 | 江西顺泉生物科技有限公司 | A kind of complex microorganism deodorant and preparation method thereof |
| CN106905003A (en) * | 2017-04-12 | 2017-06-30 | 天津海泰市政绿化有限公司 | A kind of microbial inoculum combination of city grass cuttings fermentation and its zymotechnique |
| CN108211692A (en) * | 2017-12-29 | 2018-06-29 | 广州广牧丰生物技术有限公司 | A kind of biological deodorant and its application |
| CN108144386A (en) * | 2018-02-05 | 2018-06-12 | 邱榕生 | A kind of method of poultry house air multistage cleaning deodorization |
| CN109136127A (en) * | 2018-08-22 | 2019-01-04 | 宝蓝环保科技霸州有限公司 | A kind of kitchen garbage fermentation deodorization compound bacteria and preparation method thereof |
| CN109809659A (en) * | 2019-02-22 | 2019-05-28 | 武汉益锦祥生物环保有限公司 | A kind of microbial deoderizer and its preparation method and application |
| CN109809659B (en) * | 2019-02-22 | 2021-09-07 | 湖北金旭农业资源开发有限公司 | Microbial deodorizing microbial inoculum and preparation method and application thereof |
| CN110129233A (en) * | 2019-03-15 | 2019-08-16 | 江苏省农业科学院 | A kind of microbial bacterial agent and preparation method thereof being used to prepare cage bird fermenting bed padding |
| CN110129233B (en) * | 2019-03-15 | 2022-10-04 | 江苏省农业科学院 | Microbial agent for preparing fermentation bed padding for cage-raised chickens and preparation method thereof |
| CN110038874A (en) * | 2019-04-04 | 2019-07-23 | 广西大自然再生资源有限公司 | Categorized consumer waste processing method |
| CN110024701A (en) * | 2019-04-23 | 2019-07-19 | 周宁县生隆科技养殖有限公司 | A kind of ecologically raising pigs fermentation bed and its management method |
| CN110143835A (en) * | 2019-06-10 | 2019-08-20 | 刘坚 | A method of bio-fertilizer is prepared using discarded pig fermenting bed padding |
| WO2022104772A1 (en) * | 2020-11-23 | 2022-05-27 | 南京溧水高新创业投资管理有限公司 | Cyanobacteria composting composite microbial agent and preparation method therefor |
| CN113215055A (en) * | 2021-05-31 | 2021-08-06 | 湖北金地天成农业发展有限公司 | Organic fertilizer fermentation inoculant and preparation process thereof |
| CN117229938A (en) * | 2023-05-24 | 2023-12-15 | 贵州煜宏生物科技有限公司 | Fermentation bed microbial inoculum suitable for Zhenfeng Guizhou and preparation method |
| CN117229938B (en) * | 2023-05-24 | 2024-05-10 | 贵州煜宏生物科技有限公司 | Fermentation bed microbial inoculum suitable for Zhenfeng Guizhou and preparation method |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103865860B (en) | 2016-03-02 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103865860B (en) | One boar fermentation bed tri compound microbial inoculum | |
| CN101558821B (en) | Animal feed and preparation method thereof | |
| CN104293694B (en) | A kind of preparation method of sludge aerobic compost composite bacteria agent | |
| CN101248845B (en) | Method for processing raw material of aquatic product feed stuff by fermenting | |
| CN107129953B (en) | Microbial additive for treating cattle manure and preparation method and application thereof | |
| CN105296394A (en) | Microbial decomposition agent for animal wastes and straws and preparation method thereof | |
| CN105218254A (en) | A kind of Microbial and ecological organic fertilizers material and preparation method thereof | |
| CN107325990B (en) | Composite microorganism decomposing agent and application thereof in tobacco cultivation | |
| CN101565684A (en) | Method for making fermenting bed for ecological pig breeding by using composite strain culture | |
| CN102531720A (en) | Fermented biofertilizer prepared from waste vegetables, straw and livestock and poultry feces and preparation method thereof | |
| CN106478167A (en) | Organic fermentation fertilizer and preparation method thereof | |
| CN107867939A (en) | A kind of livestock and poultry feces give up outer fermentation bed | |
| CN106554240A (en) | A kind of crops soil improving agent for one-crop succession and preparation method thereof | |
| CN112028684A (en) | Loss-control long-acting sugarcane pesticide fertilizer and preparation method thereof | |
| CN104326774A (en) | Method for preparing microbial liquid fertilizer fermented animal dung transformed organic fertilizer | |
| CN104293711A (en) | Sheep-and-cow dung fermented compound bacteria, preparation method thereof and method for preparing organic fertilizer by utilizing fermentation of compound bacteria | |
| CN111789004B (en) | Method for realizing ecological cycle by combining sugarcane field planting and breeding | |
| CN103305446A (en) | Culture medium, culture method and application of bacillus coagulans in fermentation bed | |
| CN106305487A (en) | Biological active padding material of fermentation bed for poultry, preparation method and application | |
| CN104304666A (en) | Method for producing feed by fermenting straw by compound microorganisms | |
| CN1955276A (en) | Organic microbial composite and use | |
| CN102351593A (en) | Method for preparing microbial biocontrol organic fertilizer from sludge and chaff of edible fungi | |
| KR20080019333A (en) | Method for producing microbial fermentation litter for livestock using mushroom medium | |
| CN107779416A (en) | A kind of high-efficiency fermenting bed complex micro organism fungicide | |
| CN102138637A (en) | Formula and preparation process of transforming agent of organic feed of grain-free biomass |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |