CN106047770A - Composite liquid microbial agent with long quality guarantee period and preparation method thereof - Google Patents
Composite liquid microbial agent with long quality guarantee period and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a composite liquid microbial agent with a long quality guarantee period and a preparation method thereof. The composite liquid microbial agent comprises six liquid aerobic microbial agents and four liquid anaerobic microbial agents. The liquid aerobic microbial agents comprise a liquid candida utilis microbial agent, liquid nitrobacteria, a liquid Streptomyces microflavus agent, a liquid bacillus subtilis agent, a liquid bacillus licheniformis agent and a liquid bacillus natto agent. The liquid anaerobic microbial agents comprise a liquid Rhodopseudomonas palustris agent, a liquid bifidobacterium agent, a liquid lactobacillus plantarum agent and a liquid lactobacillus acidophilus agent. The preparation method comprises culturing six types of aerobic bacteria to obtain six liquid aerobic microbial agents, culturing four types of anaerobic bacteria to obtain four mixed liquid anaerobic microbial agents, and injecting the six liquid aerobic microbial agents into the liquid anaerobic microbial agents to obtain a composite liquid microbial agent. The composite liquid microbial agent has a quality guarantee period prolonged by several times than that of the common microbial agent and has effects of decomposing dirt, purifying water and repairing an ecological environment.
Description
Technical field
The present invention relates to belong to agricultural microorganism, ecologic planting and microbial bacterial agent technical field, particularly relate to a kind of guarantor
Complex microorganism liquid bacterial agent of matter phase length and preparation method thereof.
Background technology
At present, China's animal husbandry, culture fishery abuse of antibiotics phenomenon universal, the water body polluted by excess antibiotic
By ecological circulation, cause human body bacterial drug resistance to increase, compromise health.Culture fishery is abused chemical fertilizer rich water
Phenomenon is the most universal, and it is smelly that chemical fertilizer Use out of range accelerates water body corruption, and nitrite, ammonia nitrogen, hydrogen sulfide, methane etc. are harmful
Material exceeds standard, and the aquatic products medicineization residual of cultivation exceeds standard, and quality declines, and threatens health, also causes ecological environment sternly
Heavily destroy.
Recently, National agricultural portion has been proposed for the action scheme of fertilizer and pesticide zero growth rate.Fertilizer and pesticide zero to be realized increasing
Ensure while length that agricultural product increasing both production and income realizes the effective supply of supply side again, it is necessary to can effective replacing fertilizer pesticide
Product.The microorganism formulation that it is possible to replacing fertilizer and minimizing pesticide becomes the urgent need of the current development ecological agriculture, with micro-
Biological preparation improves breeding environment, improved products quality, it is achieved increasing both production and income, it has also become the inexorable trend of modern cultivation, is
Develop the inevitable choice of green sustainable Efficient Eco-agriculture.
Adding microorganism beneficial bacterium in feedstuff, can promote that feedstuff is digested and assimilated by animal, enhancing immunity is with anti-
Sick resistance against diseases, can be greatly reduced antibiotic dosage；Interpolation microorganism beneficial bacterium in breeding water body, decomposable asymmetric choice net pollutant,
Removing toxic substances, purifies water, and increases dissolved oxygen, chemical fertilizer rich water consumption can be greatly reduced, make Ecological water environment be repaired and improve.
The most various microbial manures emerge in multitude like the mushrooms after rain, but fertilizer efficiency substantially can meet or exceed chemical fertilizer
Product the most actually rare, its main cause: is that microorganism fungus kind is single, can't resolve the problems of plant growth；Two
Being that preservative time is short, fertilizer efficiency is inconspicuous, and the most single microbial inoculum or composite bacteria agent capable are all difficult to solve liquid bacterial agent corruption, have
The problem that effect bacterium is dead, storage life is short.
Summary of the invention
Complex microorganism liquid bacterial agent that it is an object of the invention to provide a kind of long shelf-life and preparation method thereof, this is combined
Microbial liquid microbial inoculum and preparation method thereof solves that microbial bacterial agent strain is single, storage life is short and fertilizer efficiency is unconspicuous asks
Topic.
To achieve the above object of the invention, the present invention uses the complex microorganism liquid bacterial agent of a kind of long shelf-life and system thereof
The technical scheme of Preparation Method is: described complex microorganism liquid bacterial agent includes 6 kinds of liquid aerobic bacterias cultivated with fluid medium
Agent and 4 kinds of liquid anaerobic microbial inoculums cultivated with fluid medium, the aerobic microbial inoculum of described liquid and the mass ratio of liquid anaerobic microbial inoculum
For 3:7 ~ 7:3；The aerobic microbial inoculum of described liquid is made up of the microbial inoculum of following mass parts:
Liquid Candida utilis agent 1 ~ 3 mass parts,
Liquid nitric acid microbial inoculum 1 ~ 3 mass parts,
Liquid streptomyces microflavus agent 1 mass parts,
Liquid bacillus subtilis microbial inoculum 1 ~ 2 mass parts,
Liquid Bacillus licheniformis agent 1 ~ 2 mass parts,
Liquid natto bacillus cereus agent 1 ~ 2 mass parts；
Described liquid anaerobic microbial inoculum is made up of the microbial inoculum of following mass parts:
Liquid Rhodopseudomonas palustris agent 1 ~ 2 mass parts,
Liquid bifidus bacillus agent 1 ~ 2 mass parts,
Liquid plant breast microbial inoculum 1 ~ 5 mass parts,
Liquid bacillus acidophilus's agent 1 ~ 5 mass parts；
Wherein, the good bacteria content in the aerobic microbial inoculum of each liquid is more than or equal to 200,000,000/milliliter, each liquid anaerobic bacterium
Anaerobe content in agent is more than or equal to 200,000,000/milliliter.
Relevant content in technique scheme is explained as follows:
1, in such scheme, described microbial liquid microbial inoculum also includes trace element, and the addition of trace element is described compound
The 0.1 ~ 0.2% of microbial liquid microbial inoculum quality, described trace element in B, Zn, Mn, Cu, Co any one or at least two
Plant above mixture.
To achieve the above object of the invention, the preparation of the complex microorganism liquid bacterial agent of a kind of long shelf-life that the present invention uses
The technical scheme of method is: described preparation method comprises the following steps:
6 kinds of aerobic bacterias are cultivated and are made 6 kinds of aerobic microbial inoculums of liquid by the first step respectively,
(1) prepare respectively Candida utilis, Nitromonas, Bafillus natt, streptomyces microflavus, bacillus subtilis and
The liquid aerobic bacteria culture medium of Bacillus licheniformis, more respectively to 6 kinds of each self-corresponding bacterium of liquid aerobic bacteria inoculation of medium
Kind, at 30 ~ 37 DEG C, 120 ~ 140r/min, pH value carries out the one-level amplification culture of strain under the conditions of 6.5 ~ 7.5, obtains 6 kind one
The aerobic bacterium solution of level；
(2) 6 kinds of aerobic bacterium solution of one-level are taken the most respectively to 6 kinds of described each self-corresponding strains of liquid aerobic bacteria inoculation of medium,
30 ~ 37 DEG C, carry out two grades of amplification culture of strain under the conditions of 120 ~ 140r/min, obtain 6 kinds of two grades of aerobic bacterium solution, wherein,
The inoculum concentration of every kind of aerobic bacterium solution of one-level is the 0.9 ~ 1.1% of its liquid aerobic bacteria culture medium quality；
(3) 6 kinds of two grades of aerobic bacterium solution are taken again to 6 kinds of described each self-corresponding strains of liquid aerobic bacteria inoculation of medium, 30 ~
37 DEG C, carry out three grades of amplification culture of strain under the conditions of 120 ~ 140r/min, obtain 6 kinds of aerobic microbial inoculums of liquid, wherein, every kind two
Inoculum concentration is its liquid aerobic bacteria culture medium quality the 1 ~ 1.5% of the aerobic bacterium solution of level；
The liquid anaerobic microbial inoculum of mixing is made in 4 kinds of anaerobic culturees by second step,
(1) the liquid anaerobic bacterium training of Rhodopseudomonas palustris, Lactobacillus plantarum, bacillus acidophilus and bifidus bacillus is prepared respectively
Support base, then in 4 kinds of liquid anaerobic bacterium culture mediums, inoculate each self-corresponding strain respectively, stand under the conditions of 30 ~ 37 DEG C, carry out
The one-level amplification culture of strain, obtains 4 kinds of one-level anaerobism bacterium solution；
(2) take 4 kinds of one-level anaerobism bacterium solution the most respectively in 4 kinds of described liquid anaerobic bacterium culture mediums, inoculate each self-corresponding strain,
Stand under the conditions of 30 ~ 37 DEG C, carry out two grades of amplification culture of strain, obtain 4 kinds of secondary anaerobic bacterium solution, wherein, every kind of one-level
The inoculum concentration of anaerobism bacterium solution is the 0.9 ~ 1.1% of its liquid anaerobic bacterium culture medium quality；
(3) take 4 kinds of secondary anaerobic bacterium solution respectively in 4 kinds of described liquid anaerobic bacterium culture mediums, inoculate each self-corresponding strain,
Stand under the conditions of 30 ~ 37 DEG C, carry out three grades of amplification culture of strain, obtain 4 kinds of three grades of anaerobism bacterium solution, wherein, detest for two grades every kind
The inoculum concentration of oxygen bacterium solution is the 1 ~ 1.5% of its liquid anaerobic bacterium culture medium quality；
(4) by the formula proportion of a upper technical scheme, described 4 kinds of three grades of anaerobism bacterium solution are mixed directly, obtain being combined
Described liquid anaerobic microbial inoculum；
3rd step, is slowly injected into compound described liquid anaerobic by 6 kinds of aerobic microbial inoculums of liquid formula proportion according to claim 1
In microbial inoculum and stir, stand, behind isotropic stable, obtain described complex microorganism liquid bacterial agent.
Relevant content in technique scheme is explained as follows:
1, in such scheme, in described 3rd step, being simultaneously introduced trace element, the addition of trace element is described compound micro-
The 0.1 ~ 0.2% of biofluid microbial inoculum quality, described trace element is selected from B, Zn, Mn, Cu, Co any one or at least two
Above mixture.
2, in such scheme, the liquid aerobic bacteria culture medium prescription of described Candida utilis is according to shared by each component
Weight/mass percentage composition is composed of the following components: sucrose 1%, dipotassium hydrogen phosphate 0.1%, ammonium sulfate 0.2%, soluble starch 2%, carbonic acid
Calcium 0.5%, Oleum Glycines 0.5%, sterilized water 95.7%；
The liquid aerobic bacteria culture medium prescription of described Nitromonas according to the weight/mass percentage composition shared by each component by following components group
Become: peptone 1%, dipotassium hydrogen phosphate 0.1%, yeast extract 0.5%, magnesium sulfate 0.03%, sodium chloride 0.5%, sterilized water 97.87%；
The liquid aerobic bacteria culture medium prescription of described Bafillus natt according to the weight/mass percentage composition shared by each component by following
Component forms: sucrose 3%, dipotassium hydrogen phosphate 0.1%, potassium dihydrogen phosphate 0.2%, magnesium sulfate 0.2%, sterilized water 96.5%；
The liquid aerobic bacteria culture medium prescription of described streptomyces microflavus according to the weight/mass percentage composition shared by each component by following group
It is grouped into: soluble starch 2%, dipotassium hydrogen phosphate 0.05%, potassium nitrate 0.1%, magnesium sulfate 0.05%, sodium chloride 0.005%, sulphuric acid
Ferrous 0.001%, sterilized water 97.794%；
The liquid aerobic bacteria culture medium prescription of described bacillus subtilis according to the weight/mass percentage composition shared by each component by following
Component forms: sucrose 1%, dipotassium hydrogen phosphate 0.05%, yeast extract 0.05%, Carnis Bovis seu Bubali cream 0.02%, peptone 0.05%, calcium carbonate
0.025%, sodium chloride 0.02%, manganese sulfate 0.001%, sterilized water 98.784%；
The liquid aerobic bacteria culture medium prescription of described Bacillus licheniformis according to the weight/mass percentage composition shared by each component by following
Component forms: sucrose 1%, dipotassium hydrogen phosphate 0.05%, yeast extract 0.05%, Carnis Bovis seu Bubali cream 0.02%, peptone 0.05%, calcium carbonate
0.025%, sodium chloride 0.02%, manganese sulfate 0.001%, sterilized water 98.784%；
The liquid anaerobic bacterium culture medium formula of described Rhodopseudomonas palustris is: the 3.3g Han sodium acetate in every 1 liter of sterilized water, chlorination
Ammonium 0.6g, magnesium sulfate 0.5g, yeast extract 1.5g；
The liquid anaerobic bacterium culture medium formula of described Lactobacillus plantarum is: the 20g Han sucrose in every 1 liter of sterilized water, yeast extract
25g, dipotassium hydrogen phosphate 2g；
The liquid anaerobic bacterium culture medium formula of described bacillus acidophilus is: the 5g Han sodium acetate in every 1 liter of sterilized water, ammonium citrate
2g, magnesium sulfate 0.5g, yeast extract 6g, Carnis Bovis seu Bubali cream 12g, dipotassium hydrogen phosphate 2g, peptone 10g, manganese sulfate 0.05g；
The liquid anaerobic bacterium culture medium formula of described bifidus bacillus is: the 50g Han whey powder, calcium carbonate 2g in every 1 liter of sterilized water,
Yeast extract 10g.
The design feature of the present invention is: the complex microorganism liquid bacterial agent of the present invention is that aerobic flora is combined with anaerobic bacteria flora
Microbial liquid microbial inoculum, its feature is for prepare A and B two class complex micro organism fungicide the most respectively, and A microbial inoculum is aerobic by 6 kinds
Bacterium and facultative aerobe composition, every kind of aerobic bacteria uses aerobic fermentation process individually to ferment.B microbial inoculum is by 4 kinds of anaerobe and facultative
Anaerobe forms, and 4 kinds of anaerobe every kind bacterial strains use the independent anaerobic fermentation of technique of anaerobic fermentation, then by straight for 4 kinds of anaerobism bacterium solution
Connect and be mixed to get B preparation.Finally 6 kinds of A microbial inoculums are mixed in B microbial inoculum the most in proportion.This feature is advantageous in that, at aerobic
The ferment stage to 6 kinds of bacterial strain single culture, can ensure strain purity and functional on the premise of, promote aerobic bacteria quickly increase numerous
Grow.After aerobic fermentation completes, after 6 kinds of aerobic microbial inoculums of liquid are mixed in the B microbial inoculum of anaerobic fermentation in proportion, aerobic flora is subject to
To anaerobic bacteria flora and the suppression of mild acid conditions, increasing breeding and become to slow down, most bacterial strains define spore, are formed between bacterial strain
The most not environmental condition of the excellent symbiosis of antagonism, liquid bacterial agent can preserve the long period at normal temperatures.
The present invention compared with prior art, has the advantages that
(1) conventional microbial bacterial agent is under the conditions of liquid, and bacterial strain several minutes to a few hours just breeding one generation, due to the fastest
Speed flourish, bacterial strain will soon deformity dead, microbial inoculum will blackening smelly, after dilution, most floras are for this microbial inoculum
Weak through becoming deformity, it is impossible to playing its effective efficiency, under normal temperature condition, storage life is the shortest, the most only the several months.And this
In bright complex microorganism liquid bacterial agent, living bacteria count is many, long shelf-life, and two kinds of microbial inoculums of A, B ferment the most respectively
Compounding, after compounding in the environment of faintly acid, the growth conditions of flora is inhibited, and nutrient and metabolizable energy are got back simultaneously
Complementation, defines the excellent symbiotic environment of interdependent symbiosis, the most not antagonism between flora.Thus, the growth of flora is inhibited, equally
Under conditions of liquid room temperature, flora becomes slowly to grow, and most bacterial strains form spore states, and bacterial strain vigor is maintained and temporarily
Time in a dormant state.Under normal temperature condition, the complex microorganism liquid bacterial agent of the present invention preserve effect duration can reach 1 year with
On, after dilution, bacterial strain again can quickly restoration ecosystem playing a role.
(2) multi-cultur es Synergistic in the complex microorganism liquid bacterial agent of the present invention, complete function.The present invention uses A(i.e.
6 kinds of aerobic floras), B(that is 4 kind anaerobic bacteria flora) two kinds of microbial inoculums ferment respectively method the most compounding, can make more
Many difference in functionality bacterial strains compound symbiosis.Decomposing pollutant can not only be played after using, purify water, produce immunity metabolite,
Antibacterial, pest-resistant, disease prevention, makes the prominent improvement of aquatic products quality, moreover it is possible to for realizing the biological treating that water pollutes, improves ecological ring
Border, creates favorable conditions.
Detailed description of the invention
Below in conjunction with embodiment, the invention will be further described:
Embodiment 1: complex microorganism liquid bacterial agent of a kind of long shelf-life and preparation method thereof
Described complex microorganism liquid bacterial agent include 6 kinds with fluid medium cultivate the aerobic microbial inoculums of liquid and 4 kinds train with liquid
Supporting the liquid anaerobic microbial inoculum that base is cultivated, the aerobic microbial inoculum of described liquid is 11:14 with the mass ratio of liquid anaerobic microbial inoculum；Described liquid
Aerobic microbial inoculum is made up of the microbial inoculum of following mass parts:
Liquid Candida utilis agent 2 mass parts,
Liquid nitric acid microbial inoculum 2 mass parts,
Liquid streptomyces microflavus agent 1 mass parts,
Liquid bacillus subtilis microbial inoculum 2 mass parts,
Liquid Bacillus licheniformis agent 2 mass parts,
Liquid natto bacillus cereus agent 2 mass parts；
Described liquid anaerobic microbial inoculum is made up of the microbial inoculum of following mass parts:
Liquid Rhodopseudomonas palustris agent 2 mass parts,
Liquid bifidus bacillus agent 2 mass parts,
Liquid plant breast microbial inoculum 5 mass parts,
Liquid bacillus acidophilus's agent 5 mass parts；
Wherein, the good bacteria content in the aerobic microbial inoculum of each liquid is more than or equal to 200,000,000/milliliter, each liquid anaerobic bacterium
Anaerobe content in agent is more than or equal to 200,000,000/milliliter.
Described microbial liquid microbial inoculum also includes trace element, and the addition of trace element is described complex microorganism liquid
The 0.1% of microbial inoculum quality, described trace element is the mixture of B, Zn, Mn three.
Described preparation method comprises the following steps:
6 kinds of aerobic bacterias are cultivated and are made 6 kinds of aerobic microbial inoculums of liquid by the first step respectively,
(1) prepare respectively Candida utilis, Nitromonas, Bafillus natt, streptomyces microflavus, bacillus subtilis and
The liquid aerobic bacteria culture medium of Bacillus licheniformis, more respectively to 6 kinds of each self-corresponding bacterium of liquid aerobic bacteria inoculation of medium
Kind, at 30 DEG C, 120r/min, pH value carries out the one-level amplification culture of strain under the conditions of 6.5 ~ 7.5, obtains 6 kinds of one-levels aerobic
Bacterium solution；
(2) 6 kinds of aerobic bacterium solution of one-level are taken the most respectively to 6 kinds of described each self-corresponding strains of liquid aerobic bacteria inoculation of medium,
30 DEG C, carry out two grades of amplification culture of strain under the conditions of 120r/min, obtain 6 kinds of two grades of aerobic bacterium solution, wherein, every kind of one-level
The inoculum concentration of aerobic bacterium solution is the 1% of its liquid aerobic bacteria culture medium quality；
(3) 6 kinds of two grades of aerobic bacterium solution are taken again to 6 kinds of described each self-corresponding strains of liquid aerobic bacteria inoculation of medium, 30
DEG C, carry out three grades of amplification culture of strain under the conditions of 120r/min, obtain 6 kinds of aerobic microbial inoculums of liquid, wherein, two grades every kind aerobic
The inoculum concentration of bacterium solution is the 1% of its liquid aerobic bacteria culture medium quality；
The liquid anaerobic microbial inoculum of mixing is made in 4 kinds of anaerobic culturees by second step,
(1) the liquid anaerobic bacterium training of Rhodopseudomonas palustris, Lactobacillus plantarum, bacillus acidophilus and bifidus bacillus is prepared respectively
Support base, then in 4 kinds of liquid anaerobic bacterium culture mediums, inoculate each self-corresponding strain respectively, stand under the conditions of 30 DEG C, carry out bacterium
The one-level amplification culture planted, obtains 4 kinds of one-level anaerobism bacterium solution；
(2) take 4 kinds of one-level anaerobism bacterium solution the most respectively in 4 kinds of described liquid anaerobic bacterium culture mediums, inoculate each self-corresponding strain,
Stand under the conditions of 30 DEG C, carry out two grades of amplification culture of strain, obtain 4 kinds of secondary anaerobic bacterium solution, wherein, every kind of one-level anaerobism
The inoculum concentration of bacterium solution is the 1% of its liquid anaerobic bacterium culture medium quality；
(3) take 4 kinds of secondary anaerobic bacterium solution respectively in 4 kinds of described liquid anaerobic bacterium culture mediums, inoculate each self-corresponding strain,
Stand under the conditions of 30 DEG C, carry out three grades of amplification culture of strain, obtain 4 kinds of three grades of anaerobism bacterium solution, wherein, every kind of secondary anaerobic bacterium
The inoculum concentration of liquid is the 1% of its liquid anaerobic bacterium culture medium quality；
(4) by above-mentioned formula proportion, described 4 kinds of three grades of anaerobism bacterium solution are mixed directly, obtain the described liquid anaerobic being combined
Microbial inoculum；
6 kinds of aerobic microbial inoculums of liquid are slowly injected in compound described liquid anaerobic microbial inoculum by the 3rd step by above-mentioned formula proportion
And stir, stand, behind isotropic stable, obtain described complex microorganism liquid bacterial agent.
In described 3rd step, being simultaneously introduced trace element, the addition of trace element is described complex microorganism liquid
The 0.1% of microbial inoculum quality, described trace element is the mixture of B, Zn, Mn three.
The liquid aerobic bacteria culture medium prescription of described Candida utilis is according to the weight/mass percentage composition shared by each component
Composed of the following components: sucrose 1%, dipotassium hydrogen phosphate 0.1%, ammonium sulfate 0.2%, soluble starch 2%, calcium carbonate 0.5%, Oleum Glycines
0.5%, sterilized water 95.7%；
The liquid aerobic bacteria culture medium prescription of described Nitromonas according to the weight/mass percentage composition shared by each component by following components group
Become: peptone 1%, dipotassium hydrogen phosphate 0.1%, yeast extract 0.5%, magnesium sulfate 0.03%, sodium chloride 0.5%, sterilized water 97.87%；
The liquid aerobic bacteria culture medium prescription of described Bafillus natt according to the weight/mass percentage composition shared by each component by following
Component forms: sucrose 3%, dipotassium hydrogen phosphate 0.1%, potassium dihydrogen phosphate 0.2%, magnesium sulfate 0.2%, sterilized water 96.5%；
The liquid aerobic bacteria culture medium prescription of described streptomyces microflavus according to the weight/mass percentage composition shared by each component by following group
It is grouped into: soluble starch 2%, dipotassium hydrogen phosphate 0.05%, potassium nitrate 0.1%, magnesium sulfate 0.05%, sodium chloride 0.005%, sulphuric acid
Ferrous 0.001%, sterilized water 97.794%；
The liquid aerobic bacteria culture medium prescription of described bacillus subtilis according to the weight/mass percentage composition shared by each component by following
Component forms: sucrose 1%, dipotassium hydrogen phosphate 0.05%, yeast extract 0.05%, Carnis Bovis seu Bubali cream 0.02%, peptone 0.05%, calcium carbonate
0.025%, sodium chloride 0.02%, manganese sulfate 0.001%, sterilized water 98.784%；
The liquid aerobic bacteria culture medium prescription of described Bacillus licheniformis according to the weight/mass percentage composition shared by each component by following
Component forms: sucrose 1%, dipotassium hydrogen phosphate 0.05%, yeast extract 0.05%, Carnis Bovis seu Bubali cream 0.02%, peptone 0.05%, calcium carbonate
0.025%, sodium chloride 0.02%, manganese sulfate 0.001%, sterilized water 98.784%；
The liquid anaerobic bacterium culture medium formula of described Rhodopseudomonas palustris is: the 3.3g Han sodium acetate in every 1 liter of sterilized water, chlorination
Ammonium 0.6g, magnesium sulfate 0.5g, yeast extract 1.5g；
The liquid anaerobic bacterium culture medium formula of described Lactobacillus plantarum is: the 20g Han sucrose in every 1 liter of sterilized water, yeast extract
25g, dipotassium hydrogen phosphate 2g；
The liquid anaerobic bacterium culture medium formula of described bacillus acidophilus is: the 5g Han sodium acetate in every 1 liter of sterilized water, ammonium citrate
2g, magnesium sulfate 0.5g, yeast extract 6g, Carnis Bovis seu Bubali cream 12g, dipotassium hydrogen phosphate 2g, peptone 10g, manganese sulfate 0.05g；
The liquid anaerobic bacterium culture medium formula of described bifidus bacillus is: the 50g Han whey powder, calcium carbonate 2g in every 1 liter of sterilized water,
Yeast extract 10g.
The complex microorganism liquid bacterial agent of embodiment 1 contrast experiment in aquaculture:
Sample plot: 10 mu of the shrimp pool, Xi Tang township, Hepu County Guangxi.
Performance: breed variety is Penaeus vannamei.Before cultivation, rich water-put shrimp Seedling-finished product shrimp goes out pool overall process, time
Between mid-June.Experiment sets 2 cultivation pools, 5 mu of each pool.2 pools are all the old pools cultivated for many years, are covered with plastics
Film is also having pressure bed mud at the bottom of the pool.
Experimental result: experiment set 2 cultivation pools, 2 pools are all the old pools cultivated for many years, be covered with plastic foil and
Pressure bed mud, mean depth 1.5 is had at the bottom of the pool.Wherein the complex microorganism liquid bacterial agent of embodiment 1 is used in A pool, and area is 4 mu；B
Pool is the comparison pool of conventional cultivation, and area is 5 mu.B pool cultivates water quality of fostering and apply fertilizer routinely, and mu uses (depth of water 1 meter) carbonic acid
Hydrogen ammonium 20kg+ calcium superphosphate 10kg+ fertilizer 10kg, after water quality is dark brown, throws in shrimp Seedling 60,000 tails/mu.For preventing and treating prawn disease,
Splash 1 time with the full pool of the medicine such as oxytetracycline, nitrofural weekly, and in feedstuff, puddle oxytetracycline, chloromycetin, vitamin, exempt from
Epidemic disease polysaccharide, the volt multi-medicament such as iodine, Radix Et Rhizoma Rhei.At cultivation middle and late stage for purifying substrate, applicating liquid photosynthetic microbial inoculum 3 ~ 5kg/ mu (water
Deep 1 meter), every 10 days 1 time.A pool trains the liquid manure pool, same input shrimp Seedling as B pool routinely.Difference has 3 points: one
After throwing in shrimp Seedling 1 week, i.e. come to use in the complex microorganism liquid bacterial agent 3 of the embodiment 1 depositing 6 months under room temperature ~
5kg/ mu (depth of water 1 meter), splash in the full pool, every 10 days 1 time；Two is that A pool prevents and treats the medication of prawn disease and decreases half than B pool；
Three is the photosynthetic microbial inoculum that A pool does not use B pool.In breeding process, A pool is purer than B pool water colour, and disease is few, when
When prawn grows to commercial specification, through a point thin harvesting, by several times after results, ultimate output, 960 pounds/acre of the A pool, 720 pounds/acre of the B pool, the A pool
Increase by 240 pounds/acre than the B pool, increase production 33%.
Embodiment 2: complex microorganism liquid bacterial agent of a kind of long shelf-life and preparation method thereof
Described complex microorganism liquid bacterial agent include 6 kinds with fluid medium cultivate the aerobic microbial inoculums of liquid and 4 kinds train with liquid
Supporting the liquid anaerobic microbial inoculum that base is cultivated, the aerobic microbial inoculum of described liquid is 5:3 with the mass ratio of liquid anaerobic microbial inoculum；Described liquid is good
Oxygen microbial inoculum is made up of the microbial inoculum of following mass parts:
Liquid Candida utilis agent 3 mass parts,
Liquid nitric acid microbial inoculum 3 mass parts,
Liquid streptomyces microflavus agent 1 mass parts,
Liquid bacillus subtilis microbial inoculum 1 mass parts,
Liquid Bacillus licheniformis agent 1 mass parts,
Liquid natto bacillus cereus agent 1 mass parts；
Described liquid anaerobic microbial inoculum is made up of the microbial inoculum of following mass parts:
Liquid Rhodopseudomonas palustris agent 1 mass parts,
Liquid bifidus bacillus agent 1 mass parts,
Liquid plant breast microbial inoculum 2 mass parts,
Liquid bacillus acidophilus's agent 2 mass parts；
Wherein, the good bacteria content in the aerobic microbial inoculum of each liquid is more than or equal to 200,000,000/milliliter, each liquid anaerobic bacterium
Anaerobe content in agent is more than or equal to 200,000,000/milliliter.
Described microbial liquid microbial inoculum also includes trace element, and the addition of trace element is described complex microorganism liquid
The 0.2% of microbial inoculum quality, described trace element is the mixture of Zn, Mn, Cu three.
Described preparation method comprises the following steps:
6 kinds of aerobic bacterias are cultivated and are made 6 kinds of aerobic microbial inoculums of liquid by the first step respectively,
(1) prepare respectively Candida utilis, Nitromonas, Bafillus natt, streptomyces microflavus, bacillus subtilis and
The liquid aerobic bacteria culture medium of Bacillus licheniformis, more respectively to 6 kinds of each self-corresponding bacterium of liquid aerobic bacteria inoculation of medium
Kind, at 37 DEG C, 140r/min, pH value carries out the one-level amplification culture of strain under the conditions of 6.5 ~ 7.5, obtains 6 kinds of one-levels aerobic
Bacterium solution；
(2) 6 kinds of aerobic bacterium solution of one-level are taken the most respectively to 6 kinds of described each self-corresponding strains of liquid aerobic bacteria inoculation of medium,
37 DEG C, carry out two grades of amplification culture of strain under the conditions of 140r/min, obtain 6 kinds of two grades of aerobic bacterium solution, wherein, every kind of one-level
The inoculum concentration of aerobic bacterium solution is the 1.1% of its liquid aerobic bacteria culture medium quality；
(3) 6 kinds of two grades of aerobic bacterium solution are taken again to 6 kinds of described each self-corresponding strains of liquid aerobic bacteria inoculation of medium, 37
DEG C, carry out three grades of amplification culture of strain under the conditions of 140r/min, obtain 6 kinds of aerobic microbial inoculums of liquid, wherein, two grades every kind aerobic
The inoculum concentration of bacterium solution is the 1 ~ 1.5% of its liquid aerobic bacteria culture medium quality；
The liquid anaerobic microbial inoculum of mixing is made in 4 kinds of anaerobic culturees by second step,
(1) the liquid anaerobic bacterium training of Rhodopseudomonas palustris, Lactobacillus plantarum, bacillus acidophilus and bifidus bacillus is prepared respectively
Support base, then in 4 kinds of liquid anaerobic bacterium culture mediums, inoculate each self-corresponding strain respectively, stand under the conditions of 30 ~ 37 DEG C, carry out
The one-level amplification culture of strain, obtains 4 kinds of one-level anaerobism bacterium solution；
(2) take 4 kinds of one-level anaerobism bacterium solution the most respectively in 4 kinds of described liquid anaerobic bacterium culture mediums, inoculate each self-corresponding strain,
Stand under the conditions of 30 ~ 37 DEG C, carry out two grades of amplification culture of strain, obtain 4 kinds of secondary anaerobic bacterium solution, wherein, every kind of one-level
The inoculum concentration of anaerobism bacterium solution is the 1.1% of its liquid anaerobic bacterium culture medium quality；
(3) take 4 kinds of secondary anaerobic bacterium solution respectively in 4 kinds of described liquid anaerobic bacterium culture mediums, inoculate each self-corresponding strain,
Stand under the conditions of 37 DEG C, carry out three grades of amplification culture of strain, obtain 4 kinds of three grades of anaerobism bacterium solution, wherein, every kind of secondary anaerobic bacterium
The inoculum concentration of liquid is the 1.5% of its liquid anaerobic bacterium culture medium quality；
(4) described 4 kinds of three grades of anaerobism bacterium solution being mixed directly by above-mentioned formula proportion, the described liquid obtaining being combined is detested
Oxygen microbial inoculum；
6 kinds of aerobic microbial inoculums of liquid are slowly injected into compound described liquid anaerobic microbial inoculum by above-mentioned formula proportion by the 3rd step
In and stir, stand, behind isotropic stable, obtain described complex microorganism liquid bacterial agent.
In described 3rd step, being simultaneously introduced trace element, the addition of trace element is described complex microorganism liquid
The 0.1 ~ 0.2% of microbial inoculum quality, described trace element is selected from the mixture of Zn, Mn, Cu three.
The liquid aerobic bacteria culture medium prescription of described Candida utilis is according to the weight/mass percentage composition shared by each component
Composed of the following components: sucrose 1%, dipotassium hydrogen phosphate 0.1%, ammonium sulfate 0.2%, soluble starch 2%, calcium carbonate 0.5%, Oleum Glycines
0.5%, sterilized water 95.7%；
The liquid aerobic bacteria culture medium prescription of described Nitromonas according to the weight/mass percentage composition shared by each component by following components group
Become: peptone 1%, dipotassium hydrogen phosphate 0.1%, yeast extract 0.5%, magnesium sulfate 0.03%, sodium chloride 0.5%, sterilized water 97.87%；
The liquid aerobic bacteria culture medium prescription of described Bafillus natt according to the weight/mass percentage composition shared by each component by following
Component forms: sucrose 3%, dipotassium hydrogen phosphate 0.1%, potassium dihydrogen phosphate 0.2%, magnesium sulfate 0.2%, sterilized water 96.5%；
The liquid aerobic bacteria culture medium prescription of described streptomyces microflavus according to the weight/mass percentage composition shared by each component by following group
It is grouped into: soluble starch 2%, dipotassium hydrogen phosphate 0.05%, potassium nitrate 0.1%, magnesium sulfate 0.05%, sodium chloride 0.005%, sulphuric acid
Ferrous 0.001%, sterilized water 97.794%；
The liquid aerobic bacteria culture medium prescription of described bacillus subtilis according to the weight/mass percentage composition shared by each component by following
Component forms: sucrose 1%, dipotassium hydrogen phosphate 0.05%, yeast extract 0.05%, Carnis Bovis seu Bubali cream 0.02%, peptone 0.05%, calcium carbonate
0.025%, sodium chloride 0.02%, manganese sulfate 0.001%, sterilized water 98.784%；
The liquid aerobic bacteria culture medium prescription of described Bacillus licheniformis according to the weight/mass percentage composition shared by each component by following
Component forms: sucrose 1%, dipotassium hydrogen phosphate 0.05%, yeast extract 0.05%, Carnis Bovis seu Bubali cream 0.02%, peptone 0.05%, calcium carbonate
0.025%, sodium chloride 0.02%, manganese sulfate 0.001%, sterilized water 98.784%；
The liquid anaerobic bacterium culture medium formula of described Rhodopseudomonas palustris is: the 3.3g Han sodium acetate in every 1 liter of sterilized water, chlorination
Ammonium 0.6g, magnesium sulfate 0.5g, yeast extract 1.5g；
The liquid anaerobic bacterium culture medium formula of described Lactobacillus plantarum is: the 20g Han sucrose in every 1 liter of sterilized water, yeast extract
25g, dipotassium hydrogen phosphate 2g；
The liquid anaerobic bacterium culture medium formula of described bacillus acidophilus is: the 5g Han sodium acetate in every 1 liter of sterilized water, ammonium citrate
2g, magnesium sulfate 0.5g, yeast extract 6g, Carnis Bovis seu Bubali cream 12g, dipotassium hydrogen phosphate 2g, peptone 10g, manganese sulfate 0.05g；
The liquid anaerobic bacterium culture medium formula of described bifidus bacillus is: the 50g Han whey powder, calcium carbonate 2g in every 1 liter of sterilized water,
Yeast extract 10g.
The complex microorganism liquid bacterial agent of embodiment 2 contrast experiment in processing pig manure urine on pig farm
Experiment place: pool plant of In The Luchuan of Guangxi county three
Performance: this plant market pig livestock on hand 5000, wherein selecting 1 pig house is that A pig house (80 pigs) uses this
Bright complex microorganism preparations, other pig house is that B pig house is as comparison.A pig house uses the complex microorganism liquid of embodiment 2
Microbial inoculum, using method is as follows: 1, add this microbial inoculum 1% in daily ration；2, in drinking water, this microbial inoculum 1% is added；3, by this microbial inoculum
Diluting 20 times with clear water, inside and outside pig body and colony house, every day sprays 1 time；4, mix in fecaluria pond and pour this microbial inoculum 1% into every day.
After using 1 month continuously, A pig house living environment has had than B pig house and has been obviously improved, and detects through environment protection and monitoring department, stench
Lowering of concentration 97.7%, odor strength is down to less than 2.5 grades, is reached national standard reference.
Above-described embodiment, only for technology design and the feature of the explanation present invention, its object is to allow person skilled in the art
Scholar will appreciate that present disclosure and implements according to this, and 6 kinds of the most heretofore described aerobic bacterias and 4 kinds of anaerobe are each
Culture medium prescription during single culture belongs to prior art, the liquid aerobic bacteria culture medium prescription of such as Candida utilis,
The liquid aerobic bacteria culture medium prescription of Bafillus natt and the liquid anaerobic bacterium culture medium formula etc. of Rhodopseudomonas palustris,
Those skilled in the art can do proper transformation in scope disclosed above, can not limit the scope of the invention with this.
All equivalence changes made according to spirit of the invention or modification, all should contain within protection scope of the present invention.
Claims (5)
1. the complex microorganism liquid bacterial agent of a long shelf-life, it is characterised in that: described complex microorganism liquid bacterial agent includes 6
The aerobic microbial inoculum of liquid of kind fluid medium cultivation and 4 kinds of liquid anaerobic microbial inoculums cultivated with fluid medium, described liquid
Aerobic microbial inoculum is 3:7 ~ 7:3 with the mass ratio of liquid anaerobic microbial inoculum；The aerobic microbial inoculum of described liquid is by the microbial inoculum group of following mass parts
Become:
Liquid Candida utilis agent 1 ~ 3 mass parts,
Liquid nitric acid microbial inoculum 1 ~ 3 mass parts,
Liquid streptomyces microflavus agent 1 mass parts,
Liquid bacillus subtilis microbial inoculum 1 ~ 2 mass parts,
Liquid Bacillus licheniformis agent 1 ~ 2 mass parts,
Liquid natto bacillus cereus agent 1 ~ 2 mass parts；
Described liquid anaerobic microbial inoculum is made up of the microbial inoculum of following mass parts:
Liquid Rhodopseudomonas palustris agent 1 ~ 2 mass parts,
Liquid bifidus bacillus agent 1 ~ 2 mass parts,
Liquid plant breast microbial inoculum 1 ~ 5 mass parts,
Liquid bacillus acidophilus's agent 1 ~ 5 mass parts；
Wherein, the good bacteria content in the aerobic microbial inoculum of each liquid is more than or equal to 200,000,000/milliliter, each liquid anaerobic bacterium
Anaerobe content in agent is more than or equal to 200,000,000/milliliter.
The complex microorganism liquid bacterial agent of a kind of long shelf-life the most according to claim 1, it is characterised in that: described micro-life
Thing liquid bacterial agent also includes trace element, the addition of trace element be described complex microorganism liquid bacterial agent quality 0.1 ~
0.2%, described trace element is selected from B, Zn, Mn, Cu, Co the mixture of more than any one or at least two.
3. a preparation method for the complex microorganism liquid bacterial agent of long shelf-life according to claim 1, its feature exists
In: described preparation method comprises the following steps:
6 kinds of aerobic bacterias are cultivated and are made 6 kinds of aerobic microbial inoculums of liquid by the first step respectively,
(1) prepare respectively Candida utilis, Nitromonas, Bafillus natt, streptomyces microflavus, bacillus subtilis and
The liquid aerobic bacteria culture medium of Bacillus licheniformis, more respectively to 6 kinds of each self-corresponding bacterium of liquid aerobic bacteria inoculation of medium
Kind, at 30 ~ 37 DEG C, 120 ~ 140r/min, pH value carries out the one-level amplification culture of strain under the conditions of 6.5 ~ 7.5, obtains 6 kind one
The aerobic bacterium solution of level；
(2) 6 kinds of aerobic bacterium solution of one-level are taken the most respectively to 6 kinds of described each self-corresponding strains of liquid aerobic bacteria inoculation of medium,
30 ~ 37 DEG C, carry out two grades of amplification culture of strain under the conditions of 120 ~ 140r/min, obtain 6 kinds of two grades of aerobic bacterium solution, wherein,
The inoculum concentration of every kind of aerobic bacterium solution of one-level is the 0.9 ~ 1.1% of its liquid aerobic bacteria culture medium quality；
(3) 6 kinds of two grades of aerobic bacterium solution are taken again to 6 kinds of described each self-corresponding strains of liquid aerobic bacteria inoculation of medium, 30 ~
37 DEG C, carry out three grades of amplification culture of strain under the conditions of 120 ~ 140r/min, obtain 6 kinds of aerobic microbial inoculums of liquid, wherein, every kind two
Inoculum concentration is its liquid aerobic bacteria culture medium quality the 1 ~ 1.5% of the aerobic bacterium solution of level；
The liquid anaerobic microbial inoculum of mixing is made in 4 kinds of anaerobic culturees by second step,
(1) the liquid anaerobic bacterium training of Rhodopseudomonas palustris, Lactobacillus plantarum, bacillus acidophilus and bifidus bacillus is prepared respectively
Support base, then in 4 kinds of liquid anaerobic bacterium culture mediums, inoculate each self-corresponding strain respectively, stand under the conditions of 30 ~ 37 DEG C, carry out
The one-level amplification culture of strain, obtains 4 kinds of one-level anaerobism bacterium solution；
(2) take 4 kinds of one-level anaerobism bacterium solution the most respectively in 4 kinds of described liquid anaerobic bacterium culture mediums, inoculate each self-corresponding strain,
Stand under the conditions of 30 ~ 37 DEG C, carry out two grades of amplification culture of strain, obtain 4 kinds of secondary anaerobic bacterium solution, wherein, every kind of one-level
The inoculum concentration of anaerobism bacterium solution is the 0.9 ~ 1.1% of its liquid anaerobic bacterium culture medium quality；
(3) take 4 kinds of secondary anaerobic bacterium solution respectively in 4 kinds of described liquid anaerobic bacterium culture mediums, inoculate each self-corresponding strain,
Stand under the conditions of 30 ~ 37 DEG C, carry out three grades of amplification culture of strain, obtain 4 kinds of three grades of anaerobism bacterium solution, wherein, detest for two grades every kind
The inoculum concentration of oxygen bacterium solution is the 1 ~ 1.5% of its liquid anaerobic bacterium culture medium quality；
(4) described 4 kinds of three grades of anaerobism bacterium solution are mixed directly by the formula proportion according to claim 1, and obtain being combined is described
Liquid anaerobic microbial inoculum；
3rd step, is slowly injected into compound described liquid anaerobic by 6 kinds of aerobic microbial inoculums of liquid formula proportion according to claim 1
In microbial inoculum and stir, stand, behind isotropic stable, obtain described complex microorganism liquid bacterial agent.
The preparation method of the complex microorganism liquid bacterial agent of a kind of long shelf-life the most according to claim 3, its feature exists
In: in described 3rd step, being simultaneously introduced trace element, the addition of trace element is described complex microorganism liquid bacterial agent matter
The 0.1 ~ 0.2% of amount, described trace element is selected from B, Zn, Mn, Cu, Co the mixture of more than any one or at least two.
The preparation method of the complex microorganism liquid bacterial agent of a kind of long shelf-life the most according to claim 3, its feature exists
In: the liquid aerobic bacteria culture medium prescription of described Candida utilis according to the weight/mass percentage composition shared by each component by following
Component forms: sucrose 1%, dipotassium hydrogen phosphate 0.1%, ammonium sulfate 0.2%, soluble starch 2%, calcium carbonate 0.5%, Oleum Glycines 0.5%, nothing
Bacterium water 95.7%；
The liquid aerobic bacteria culture medium prescription of described Nitromonas according to the weight/mass percentage composition shared by each component by following components group
Become: peptone 1%, dipotassium hydrogen phosphate 0.1%, yeast extract 0.5%, magnesium sulfate 0.03%, sodium chloride 0.5%, sterilized water 97.87%；
The liquid aerobic bacteria culture medium prescription of described Bafillus natt according to the weight/mass percentage composition shared by each component by following
Component forms: sucrose 3%, dipotassium hydrogen phosphate 0.1%, potassium dihydrogen phosphate 0.2%, magnesium sulfate 0.2%, sterilized water 96.5%；
The liquid aerobic bacteria culture medium prescription of described streptomyces microflavus according to the weight/mass percentage composition shared by each component by following group
It is grouped into: soluble starch 2%, dipotassium hydrogen phosphate 0.05%, potassium nitrate 0.1%, magnesium sulfate 0.05%, sodium chloride 0.005%, sulphuric acid
Ferrous 0.001%, sterilized water 97.794%；
The liquid aerobic bacteria culture medium prescription of described bacillus subtilis according to the weight/mass percentage composition shared by each component by following
Component forms: sucrose 1%, dipotassium hydrogen phosphate 0.05%, yeast extract 0.05%, Carnis Bovis seu Bubali cream 0.02%, peptone 0.05%, calcium carbonate
0.025%, sodium chloride 0.02%, manganese sulfate 0.001%, sterilized water 98.784%；
The liquid aerobic bacteria culture medium prescription of described Bacillus licheniformis according to the weight/mass percentage composition shared by each component by following
Component forms: sucrose 1%, dipotassium hydrogen phosphate 0.05%, yeast extract 0.05%, Carnis Bovis seu Bubali cream 0.02%, peptone 0.05%, calcium carbonate
0.025%, sodium chloride 0.02%, manganese sulfate 0.001%, sterilized water 98.784%；
The liquid anaerobic bacterium culture medium formula of described Rhodopseudomonas palustris is: the 3.3g Han sodium acetate in every 1 liter of sterilized water, chlorination
Ammonium 0.6g, magnesium sulfate 0.5g, yeast extract 1.5g；
The liquid anaerobic bacterium culture medium formula of described Lactobacillus plantarum is: the 20g Han sucrose in every 1 liter of sterilized water, yeast extract
25g, dipotassium hydrogen phosphate 2g；
The liquid anaerobic bacterium culture medium formula of described bacillus acidophilus is: the 5g Han sodium acetate in every 1 liter of sterilized water, ammonium citrate
2g, magnesium sulfate 0.5g, yeast extract 6g, Carnis Bovis seu Bubali cream 12g, dipotassium hydrogen phosphate 2g, peptone 10g, manganese sulfate 0.05g；
The liquid anaerobic bacterium culture medium formula of described bifidus bacillus is: the 50g Han whey powder, calcium carbonate 2g in every 1 liter of sterilized water,
Yeast extract 10g.
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