CN107129953A - A kind of microbe additive and preparation method and application for ox treatment for cow manure - Google Patents

A kind of microbe additive and preparation method and application for ox treatment for cow manure Download PDF

Info

Publication number
CN107129953A
CN107129953A CN201710473905.4A CN201710473905A CN107129953A CN 107129953 A CN107129953 A CN 107129953A CN 201710473905 A CN201710473905 A CN 201710473905A CN 107129953 A CN107129953 A CN 107129953A
Authority
CN
China
Prior art keywords
parts
bacterium powder
cfu
bacterium
powder
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CN201710473905.4A
Other languages
Chinese (zh)
Other versions
CN107129953B (en
Inventor
王诚
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Shandong Jianyuan Biotechnology Co., Ltd.
Original Assignee
王诚
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 王诚 filed Critical 王诚
Priority to CN201710473905.4A priority Critical patent/CN107129953B/en
Publication of CN107129953A publication Critical patent/CN107129953A/en
Application granted granted Critical
Publication of CN107129953B publication Critical patent/CN107129953B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20Bacteria; Culture media therefor
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K1/00Housing animals; Equipment therefor
    • A01K1/015Floor coverings, e.g. bedding-down sheets ; Stable floors
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05FORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F17/00Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05FORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F3/00Fertilisers from human or animal excrements, e.g. manure
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14Fungi; Culture media therefor
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02WCLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00Technologies for solid waste management
    • Y02W30/40Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse

Abstract

The invention belongs to treatment for cow manure technical field, more particularly to a kind of microbe additive and preparation method and application for ox treatment for cow manure.The microbe additive active component for being used for ox treatment for cow manure is made up of following raw material:Trichoderma reesei bacterium powder, aspergillus niger bacterium powder, cellulase, sporotrichum thermophile, Trichoderma viride bacterium powder, koning trichoderma bacterium solution, denitrifying bacteria microbial inoculum, bacillus megaterium bacterium powder, Bacillus licheniformis powder, Rhodopseudomonas palustris bacterium powder, jelly bacillus bacterium powder, white light gray streptomycete bacterium powder, streptomyces tendae bacterium powder, thermoactinomyces sacchari bacterium powder, dark red rotation silk actinomyces bacterium powder, streptomyces roseochromogenes bacterium powder, cyromazine, potassium fulvate, diatomite.This is used for the microbe additive reasonable recipe of ox treatment for cow manure, preparation is simple, the decomposition of original strain, reinforcement to organic matter can be supplemented in compost, can improve crop nutrition, improved soil structure, permeability, the fertilizer conservation water conservation for improving soil, environmental protection.

Description

A kind of microbe additive and preparation method and application for ox treatment for cow manure
(1) technical field
The invention belongs to treatment for cow manure technical field, more particularly to a kind of microbe additive for ox treatment for cow manure and Preparation method and application.
(2) background technology
With the development of national economy, the continuous improvement of living standards of the people, requirement of the people to food is by plant type Animal-type is turned to, requires that food has trophism and unique local flavor from being satisfied with to have enough to eat and wear to change into, thus aquaculture obtains Fast development, especially in the 1980s, since " vegetable basket project " is carried out by country, animal husbandry is solving urban and rural residents The self-supporting aspect of meat, egg, milk, plays a significantly greater role.But substantial amounts of feces of livestock and poultry not only causes serious environmental pollution, and The serious sustainable and stable development for constraining animal husbandry.
In environmental protection consciousness increasingly enhanced today, the following pollution problems of people's common concern:First, feces of livestock and poultry pair The pollution of environment;2nd, the pollution of nitrogen and phosphorus:Compound containing substantial amounts of nitrogen and phosphorus in feces of livestock and poultry, especially in feed Amino acid imbalance, using nutrient it is low in the case of, content is higher, and these nitrogen and phosphorus enter after soil, can be converted into nitric acid Salt and phosphate, too high levels can make soil lose productive value, cause the pollution of surface water and groundwater, make nitre state in water Nitrogen, hardness and total number of bacteria are exceeded.After body eutrophication, mosquitos and flies and other insects largely multiply, algae and other aquatic plants Thing amount reproduction, reduces oxygen in water, and the aquatic animal such as fishes and shrimps is dead because of anoxic.
Cow dung is the major pollutants during milk cattle cultivating, and the processing major way of existing cow dung excrement is sent out including compost Ferment method.The main degradation using microorganism into fertilizer or fuel, resource is re-used cattle manure.According to oxygen The supply situation of gas, is divided into aerobic fermentation and anaerobic fermentation by fermentation, both can the organic matter decomposition in discarded object into CO2、H2O and organic fertilizer or bio-fuel etc., and release big calorimetric.The activity of microorganism is to whole Composting It is vital.In composting process, whole microbial community structure develops rapidly alternating with the progress of compost, is that microorganism lives The dynamic result for interacting and influenceing with compost index (temperature, moisture, pH, carbon-nitrogen ratio etc.).Traditional compost is utilized in raw material Indigenous microorganism carrys out degradation of organic substances, because the firm incipient stage functional microorganism quantity of compost is few, causes fermentation period long, simultaneously Also the loss of nitrogen is easily caused, so as to pollute environment;Moreover, being influenceed by composting cycle and condition, micro- life in composting process Thing survival rate is low, and night soil-treatment effect is poor, the need for the fertilizer that compost is obtained can not meet plant growth well to nutrient.
(3) content of the invention
The present invention in order to make up the deficiencies in the prior art there is provided a kind of reasonable recipe, prepare it is simple, in composting process Original strain can be supplemented, strengthen decomposition to organic matter, as fertilizer can improve using ground crop nutrition, improved soil structure, Permeability, the fertilizer conservation water conservation of raising soil, the environmentally friendly microbe additive and preparation method and application for ox treatment for cow manure, It is adapted to modern large-scale breeding enterprise processing cow dung excrement dirty, solves problems of the prior art.
The present invention is achieved through the following technical solutions:
A kind of microbe additive for ox treatment for cow manure, its active component is prepared from the following raw materials in parts by weight:
It is 1-5 parts of trichoderma reesei bacterium powder, 8-12 parts of aspergillus niger bacterium powder, 8-12 parts of cellulase, 1-3 parts of sporotrichum thermophile, green 1-3 parts of color trichoderma bacterium powder, 1-3 parts of koning trichoderma bacterium powder, 2-6 parts of denitrifying bacteria microbial inoculum, 1-3 parts of bacillus megaterium bacterium powder, 0.5-2 parts of Bacillus licheniformis powder, 2-6 parts of Rhodopseudomonas palustris bacterium powder, 2-6 parts of jelly bacillus bacterium powder, white light gray 1-3 parts of streptomycete bacterium powder, 1-3 parts of streptomyces tendae bacterium powder, 0.5-1.5 parts of thermoactinomyces sacchari bacterium powder, dark red rotation silk actinomyces 0.5-2 parts of bacterium powder, 0.5-2 parts of streptomyces roseochromogenes bacterium powder, 1 part of cyromazine, 1-3 parts of potassium fulvate, 40-45 parts of diatomite.
It is preferred that, this is used for the microbe additive of ox treatment for cow manure, its active component by following parts by weight raw material It is made:
3 parts of trichoderma reesei, 10 parts of aspergillus niger, 10 parts of cellulase, 2 parts of sporotrichum thermophile, 2 parts of Trichoderma viride, Kang Shi wood 2 parts of mould liquid, 4 parts of denitrifying bacteria, 2 parts of bacillus megaterium, 1 part of bacillus licheniformis, 4 parts of Rhodopseudomonas palustris, glue 1 part of 4 parts of bacillus of jelly, white 2 parts of light gray streptomycete bacterium powder, 2 parts of streptomyces tendae bacterium powder and thermoactinomyces sacchari bacterium powder, Dark red 1 part of rotation silk actinomyces bacterium powder, 1 part of streptomyces roseochromogenes bacterium powder, 1 part of cyromazine, 2 parts of potassium fulvate, 43 parts of diatomite.
This is used for the preparation method of the microbe additive of ox treatment for cow manure, including following operating procedure:Take above-mentioned weight Each raw material of number, is mixed and stirred for uniformly, 24h being stood under the conditions of 28-32 DEG C, dispenses, produce;
Wherein:The viable count index of each raw material is:Trichoderma reesei 2.0 × 109Cfu/g, aspergillus niger 1.5 × 1010Cfu/g, sporotrichum thermophile 1.0 × 109Cfu/g, Trichoderma viride 2.0 × 109Cfu/g, koning trichoderma 2.0 × 109Cfu/g, Denitrifying bacteria 1.0 × 1010Cfu/g, bacillus megaterium 2.0 × 1010Cfu/g, bacillus licheniformis 2.0 × 1010Cfu/g, Rhodopseudomonas palustris 1.0 × 1010Cfu/g, jelly bacillus 1.0 × 1010Cfu/g, white light gray streptomycete bacterium powder 1.0 × 109Cfu/g, streptomyces tendae bacterium powder 2.0 × 108Cfu/g, and thermoactinomyces sacchari bacterium powder 2.0 × 108Cfu/g, dark red rotation Silk actinomyces bacterium powder 2.0 × 108Cfu/g, streptomyces roseochromogenes bacterium powder 2.0 × 108cfu/g。
This is used for the microbe additive of ox treatment for cow manure and raises the application on bed in milk cow:Addition is used for ox treatment for cow manure The fermented cow dung of microbe additive dirty used as cow dung poultry bed.It is used for the microbe additive heap of ox treatment for cow manure through this The treated solid cow dung of fertilizer is also a kind of selection well as milk cow fermenting bed padding, had both solved the dirty pollution of excrement and has asked Topic, solves the source problem that comes of ox bed padding, had both opened up the utilization channel of cow dung, ox bed padding cost, one are reduced again again Lift obtain more.
Each bacterium bacterium powder and bacterium solution used in the present invention with the addition of through certain content mixture and can decompose various macromolecular complex The enzyme of matter, and cyromazine, potassium fulvate, diatomite, stand through 24h thermophilics, can fully adapt to material environment after mixing, apply For in pending cow dung dirt, can effectively prevent and treat the disease in cow dung dirt or suppress pathogenic microorganism, and a variety of tools can be produced There is the enzyme system of bioactivity, quickly the harmful microorganism and disease pest in kill excrement, and decompose larger molecular organicses and form easy suction The organic or inorganic molecules of receipts.Wherein, there is the koning trichoderma spore that koning trichoderma is provided very strong pathogenic microorganism to suppress work( Energy;The activation Trichoderma viride that Trichoderma viride bacterium powder is provided can produce cellulase, chitinase, zytase etc.;Trichoderma reesei The activation trichoderma reesei that bacterium powder is provided produces cellulase, hemicellulase, protease, amylase;Aspergillus niger has cracking big Molecular organic and indissoluble inorganic matter, can produce amylase, acid protease, cellulase, pectase, glucose oxidase, Citric acid, gluconic acid and gallic acid etc..These moulds are in manure fermentation processing and improve soil texture, strengthen soil fertility In have great importance.Each actinomyces actinoides used in the present invention can produce a variety of enzymes such as cellulase, in the excrement that can degrade Various insoluble organic substances generate various nutrient molecules, to the mineralising of organic matter also important role, so as to purify ring Border, improved soil, have a positive effect in muck fermentation.
Thermoactinomyces sacchari and sporotrichum thermophile used in the present invention belong to typical high temperature fiber and decompose strain, can be fast Speed improves the temperature of muck, and at 40-50 DEG C can the plain enzymes of a large amount of eccrine fibers, by the quick decomposition of the organic matters such as feces of livestock and poultry into Organic matter.The microbe additive is with the rise of temperature in the dirty composting process of cow dung, it may appear that recovery, breeding accelerated growth and The process withered away after final metabolism, the beneficial metabolic product that whole process is produced fully meet cow dung it is dirty in harmful substance and have The removal and decomposition of machine material.In addition, when during excrement layer heap temperature up to 55-65 DEG C, up to 70 DEG C when Become thoroughly decomposed under effect, not only eliminate stink, it is often more important that high temperature can kill germ, worm's ovum, grass-seed in compost, largely have Machine pollution can be eliminated.On this condition, final nearly all microorganism is stopped action, and makes fermentation muck nutriment good Preserve.
The microbe additive for being used for ox treatment for cow manure is a kind of complex microorganism preparations, the environment friend containing high concentration Microorganism in good type microorganism, product can produce the protein that digestive ferment comes in decomposing and fermenting compost in composting process Grain and organic matter.Original strain can be supplemented in composting process, strengthens the decomposition to organic matter to utilize excrement composting.Make excrement Just a large amount of beneficial microbes are provided after fermenting, decomposing organic matter, activation indissoluble mineral element improve crop nutrition, promote crop Growth, strengthens resistance against diseases, and improved soil structure improves the permeability of soil, fertilizer conservation, water conservation.
To sum up, beneficial effect of the present invention for the microbe additive of ox treatment for cow manure:
1st, greatly reduce the input of chemical fertilizer, shorten the fermentation time of compost;Reduce cost.
2nd, the land pollution caused by the dirty leachate of cow dung and water pollution are reduced;It can be used as the administration in farmland or vegetable plot Fertilizer.
3rd, can be to connect treatment for cow manure and improve the output of milk, save the knob of bedding and padding spending as milk cow fermenting bed padding Band, economic benefit is clearly.
Usage and dosage:With 5-8% weight ratio, the microorganism for ox treatment for cow manure is added into the dirt of pending cow dung Additive is used.
(4) embodiment
With reference to embodiment, the invention will be further described, but the invention is not limited in this.
Embodiment 1:
This is used for the microbe additive of ox treatment for cow manure, and its active component is made up of the raw material of following weight:
Trichoderma reesei 3g, aspergillus niger 10g, cellulase 10g, sporotrichum thermophile 2g, Trichoderma viride 2g, koning trichoderma bacterium powder 2g, denitrifying bacteria microbial inoculum 4g, bacillus megaterium bacterium powder 2g, Bacillus licheniformis powder 1g, Rhodopseudomonas palustris bacterium powder 4g, jelly bacillus bacterium powder 4g, white light gray streptomycete 2g, streptomyces tendae bacterium powder 2g and thermoactinomyces sacchari bacterium powder 1g, Dark red rotation silk actinomyces bacterium powder 1g, streptomyces roseochromogenes bacterium powder 1g, cyromazine 1g, potassium fulvate 2g, diatomite 43g;
This is used for the preparation method of the microbe additive of ox treatment for cow manure, including following operating procedure:
Each raw material of above-mentioned weight is taken, is mixed and stirred for uniformly, 24h being stood under the conditions of 28-32 DEG C, dispenses, produce.
Usage and dosage:With 5-8% weight ratio, the microorganism for ox treatment for cow manure is added into the dirt of pending cow dung Additive is used.
Embodiment 2:
This is used for the microbe additive of ox treatment for cow manure, and its active component is made up of the raw material of following weight:
Trichoderma reesei bacterium powder 1g, aspergillus niger bacterium powder 8g, cellulase 8g, sporotrichum thermophile 1g, Trichoderma viride bacterium powder 1g, health Family name's trichoderma bacterium powder 1g, denitrifying bacteria microbial inoculum 2g, bacillus megaterium bacterium powder 1g, Bacillus licheniformis powder 0.5g, marsh are red Pseudomonad bacterium powder 2g, jelly bacillus bacterium powder 2g, white light gray streptomycete bacterium powder 1g, streptomyces tendae bacterium powder 1g, sugarcane are blue Uncommon Salmonella bacterium powder 0.5g, dark red rotation silk actinomyces bacterium powder 0.5g, streptomyces roseochromogenes bacterium powder 0.5g, 1 part of cyromazine, fulvic acid Potassium 1g, diatomite 40g.
Preparation method and usage and dosage be the same as Example 1.
Embodiment 3
This is used for the microbe additive of ox treatment for cow manure, and its active component is made up of the raw material of following weight:
Trichoderma reesei bacterium powder 5g, aspergillus niger bacterium powder 12g, cellulase 12g, sporotrichum thermophile 3g, Trichoderma viride bacterium powder 3g, Koning trichoderma bacterium powder 3g, denitrifying bacteria microbial inoculum 6g, bacillus megaterium bacterium powder 3g, Bacillus licheniformis powder 2g, marsh are red Pseudomonad bacterium powder 6g, jelly bacillus bacterium powder 6g, white light gray streptomycete bacterium powder 3g, streptomyces tendae bacterium powder 3g, sugarcane are blue Uncommon Salmonella bacterium powder 1.5g, dark red rotation silk actinomyces bacterium powder 2g, streptomyces roseochromogenes bacterium powder 2g, cyromazine 1g, potassium fulvate 3g, Diatomite 45g.
Preparation method and usage and dosage be the same as Example 1.
The viable count index of foregoing each raw material is:Trichoderma reesei 2.0 × 109Cfu/g, aspergillus niger 1.5 × 1010Cfu/g, it is thermophilic Hot side spore mould 1.0 × 109Cfu/g, Trichoderma viride 2.0 × 109Cfu/g, koning trichoderma 2.0 × 109Cfu/g, denitrifying bacteria 1.0×1010Cfu/g, bacillus megaterium 2.0 × 1010Cfu/g, bacillus licheniformis 2.0 × 1010Cfu/g, marsh is red false single Born of the same parents bacterium 1.0 × 1010Cfu/g, jelly bacillus 1.0 × 1010Cfu/g, white light gray streptomycete bacterium powder 1.0 × 109Cfu/g, Tang Moral streptomycete bacterium powder 2.0 × 108Cfu/g, and thermoactinomyces sacchari bacterium powder 2.0 × 108Cfu/g, dark red rotation silk actinomyces bacterium powder 2.0×108Cfu/g, streptomyces roseochromogenes bacterium powder 2.0 × 108cfu/g。
Above-mentioned each bacterium bacterium powder, microbial inoculum are common commercially available microbial inoculum product.
Experimental observation
First, the microbe additive for ox treatment for cow manure of the present invention is used in cow dung compost, studies it to compost The influence of fermentation.
1st, the cow dung that with the addition of the present invention for the microbe additive of ox treatment for cow manure is dirty, and compost temperature was in the 7th day temperature Degree rises to 69 DEG C, and the 11st day temperature of the dirty compost control group of cow dung for being not added with any microbial inoculum compost is just 63 DEG C, individually inoculation The 9th day up to 66 DEG C of the temperature of compost of cellulose-degrading bacteria L-06 processing;Above-mentioned each bacterium powder or microbial inoculum, the 9th is used alone Up to 50 DEG C of its temperature;Using above-mentioned wantonly 2 kinds of microbial inoculums, prepared using the preparation method of the present invention, in the 7th day temperature highest Rise to 45 DEG C;Using above-mentioned wantonly 3 kinds of microbial inoculums, prepared using the preparation method of the present invention, in the 7th day up to 50 DEG C of temperature; Using above-mentioned wantonly 4 kinds of microbial inoculums, prepared using the preparation method of the present invention, in the 7th day up to 49 DEG C of temperature;Use above-mentioned 5 kinds of microbial inoculums, are prepared using the preparation method of the present invention, in the 7th day up to 55 DEG C of temperature;Using above-mentioned wantonly 6 kinds of microbial inoculums, adopt Prepared with the preparation method of the present invention, in the 7th day up to 52 DEG C of temperature;Using above-mentioned wantonly 7 kinds of microbial inoculums, using the present invention's Prepared by preparation method, in the 7th day up to 55 DEG C of temperature;Using above-mentioned wantonly 8 kinds of microbial inoculums, using the preparation method system of the present invention It is standby, in the 7th day up to 52 DEG C of temperature;Using above-mentioned wantonly 9 kinds of microbial inoculums, prepared using the preparation method of the present invention, at the 7th day Up to 50 DEG C of temperature;Using above-mentioned wantonly 10 kinds of microbial inoculums, prepared using the preparation method of the present invention, in the 7th day temperature highest Rise to 56 DEG C;Using above-mentioned wantonly 11 kinds of microbial inoculums, prepared using the preparation method of the present invention, in the 7th day up to 52 DEG C of temperature; Using above-mentioned wantonly 12 kinds of microbial inoculums, prepared using the preparation method of the present invention, in the 7th day up to 50 DEG C of temperature;Using above-mentioned Wantonly 13 kinds of microbial inoculums, are prepared using the preparation method of the present invention, in the 7th day up to 60 DEG C of temperature;Use above-mentioned wantonly 14 kinds of bacterium Agent, is prepared using the preparation method of the present invention, in the 7th day up to 54 DEG C of temperature;I.e. maximum temperature is not only advanced by also Risen, cycle time.
2nd, the cow dung that with the addition of the present invention for the microbe additive of ox treatment for cow manure is dirty, and compost moisture content becomes in decline Gesture, decrease speed is faster than the dirty compost control group of cow dung, compared to the compost moisture content of independent inoculation cellulose-degrading bacteria L-06 processing Decline fast;Moreover, compost moisture content decrease speed is compared to being used alone above-mentioned each single bacterium processing, above-mentioned wantonly 2 kinds of microbial inoculums It is used in mixed way processing, the be used in mixed way processing, above-mentioned wantonly 4 kinds of microbial inoculums of above-mentioned wantonly 3 kinds of microbial inoculums are used in mixed way processing, above-mentioned wantonly 5 Kind of microbial inoculum be used in mixed way processing, above-mentioned wantonly 6 kinds of microbial inoculums be used in mixed way processing, above-mentioned wantonly 7 kinds of microbial inoculums be used in mixed way processing, The be used in mixed way processing, above-mentioned wantonly 9 kinds of microbial inoculums of above-mentioned wantonly 8 kinds of microbial inoculums are used in mixed way processing, the mixing of above-mentioned wantonly 10 kinds of microbial inoculums Using processing, above-mentioned wantonly 11 kinds of microbial inoculums be used in mixed way processing, above-mentioned wantonly 12 kinds of microbial inoculums be used in mixed way processing, above-mentioned wantonly 13 kinds Microbial inoculum be used in mixed way processing, the compost moisture content for being used in mixed way processing of above-mentioned wantonly 14 kinds of microbial inoculums decline it is fast.
Above-mentioned moisture content trend, which determines to use to determine the dirty water content of cow dung at interval of 2 days and carry out curve tracing, observes aqueous Rate decrease speed.
3rd, the cow dung that with the addition of the present invention for the microbe additive of ox treatment for cow manure is dirty, and C/N fall is maximum; Individually inoculation cellulose-decomposing bacterium L-06 processing is more or less the same with the dirty compost control group fall of cow dung;It is used alone above-mentioned each Single microbial inoculum, using above-mentioned wantonly 2 kinds of microbial inoculums, using above-mentioned wantonly 3 kinds of microbial inoculums, using above-mentioned wantonly 4 kinds of microbial inoculums, use above-mentioned wantonly 5 kinds Microbial inoculum, using above-mentioned wantonly 6 kinds of microbial inoculums, use above-mentioned wantonly 7 kinds of microbial inoculums, wantonly 8 kinds of microbial inoculums, wantonly 9 kinds of microbial inoculums, wantonly 10 kinds of microbial inoculums, wantonly 11 Microbial inoculum, wantonly 12 kinds of microbial inoculums, wantonly 13 kinds of microbial inoculums, the processing of wantonly 14 kinds of microbial inoculums are planted, is differed not with the dirty compost control group fall of cow dung Greatly.
4th, the cow dung that with the addition of the present invention for the microbe additive of ox treatment for cow manure is dirty, and ammonium nitrogen content reaches in 7d To peak value 2.96g/kg, 1.29g/kg is reduced to after compost terminates, 1.67g/kg is have dropped than maximum;Individually it is inoculated with cellulose The dirty ammonium nitrogen content of cow dung of decomposer L-06 processing reaches maximum 2.82g/kg in 10d, and compost terminates rear ammonium nitrogen and contained Measure as 0.64g/kg, 2.18g/kg is have dropped than maximum;After fermentation ends, the present invention is with the addition of for the micro- of ox treatment for cow manure The dirty nitrate nitrogen content of the cow dung of bio-additive reaches 5.52g/kg, is 6.67 times before fermentation;Individually it is inoculated with cellulose decomposition Bacterium L-06 cow dung is dirty and the dirty compost control group of cow dung is respectively 2.12g/kg and 2.10g/kg, 2.94 Hes before only fermenting 2.92 again.
2nd, cow house environment is to ensure milk cow health, improve the key factor of production capacity, and poultry bed has special to milk cow Important meaning, the time that milk cow there are about 50%-60% daily lies prone and bed rest and ruminated, and the CBF that mammary gland is flowed through when lying prone sleeping can Increase 20%-25%, so that have additional nutrients efficiency and the output of milk, bad poultry bed environment can be such that the sleeping time of lying prone of milk cow substantially subtracts It is few, milk cow rest and the output of milk are had a strong impact on, cutting improves the disease incidences such as limping, toe hoof disease and mammitis.
Experiment mastitis for milk cows disease condition under different poultry bed padding environment counts as shown in the table, clinic mastitis The incidence of disease raises bed highest with dry cow dung, fermented cow dung, common of the addition present invention for the microbe additive of ox treatment for cow manure Compost cow dung and sandy soil poultry bed take second place, and rubber blanket poultry bed is minimum;Recessive mastitis incidence of disease incidence of disease dry cow dung raises bed highest, adds Plus the fermented cow dung poultry bed of the present invention, as sandy soil poultry bed, rubber blanket poultry bed is minimum.
Input cost is adjusted:By taking the 2000 cow head numbers of animals raised as an example, rubber blanket existing market price at every about 500 yuan, Service life is generally 5 years, needs within 10 years input cost to be about 2,000,000 yuan using rubber blanket as poultry bed.Cow dung separating dry space from moist space Equipment price is about 1,200,000 yuan, is calculated with 20 years service life, annual cost is about 60,000 yuan, and annual maintenance cost is about 50,000 Member, the electricity charge are about 20,000 yuan, the cow dung of separating dry space from moist space after harmless treatment in addition to being used every year for this as poultry bed padding, Other purposes can be used for export trade, such as organic fertilizer and plantation White mushroom, profit perannum is about 100,000 yuan, uses addition The present invention as poultry bed needs input cost about in 10 years for the dirty fermentation material of cow dung of the microbe additive of ox treatment for cow manure For 300,000 yuan.Annual cost is about 40,000 yuan to sandy soil on demand, because sandy soil as bedding and padding can not use automatic dung cleaning system, Artificial cleaning up excrement is needed, the 2000 cow head numbers of animals raised need manually about 10 people, and wage is about 50,000 yuan for each person every year, and poultry is used as using sandy soil Bed needs input cost to be about 900,000 yuan for 10 years, it can be seen that, it is used for the microorganism of ox treatment for cow manure using with the addition of the present invention The dirty fermentation material of the cow dung of additive will be well below common compost cow dung rubber blanket and sandy soil as poultry bed padding cost.

Claims (4)

1. a kind of microbe additive for ox treatment for cow manure, it is characterized in that:Its active component by following parts by weight original Material is made:
1-5 parts of trichoderma reesei bacterium powder, 8-12 parts of aspergillus niger bacterium powder, 8-12 parts of cellulase, 1-3 parts of sporotrichum thermophile, green wood 1-3 parts of mould powder, 1-3 parts of koning trichoderma bacterium powder, 2-6 parts of denitrifying bacteria microbial inoculum, 1-3 parts of bacillus megaterium bacterium powder, lichens 0.5-2 parts of bacillus bacterium powder, 2-6 parts of Rhodopseudomonas palustris bacterium powder, 2-6 parts of jelly bacillus bacterium powder, white light gray strepto- 1-3 parts of bacterium bacterium powder, 1-3 parts of streptomyces tendae bacterium powder, 0.5-1.5 parts of thermoactinomyces sacchari bacterium powder, dark red rotation silk actinomyces bacterium powder 0.5-2 parts, 0.5-2 parts of streptomyces roseochromogenes bacterium powder, 1 part of cyromazine, 1-3 parts of potassium fulvate, 40-45 parts of diatomite.
2. a kind of microbe additive for ox treatment for cow manure according to claim 1, it is characterized in that:Its active component It is prepared from the following raw materials in parts by weight:
3 parts of trichoderma reesei, 10 parts of aspergillus niger, 10 parts of cellulase, 2 parts of sporotrichum thermophile, 2 parts of Trichoderma viride, koning trichoderma bacterium 2 parts of powder, 4 parts of denitrifying bacteria microbial inoculum, 2 parts of bacillus megaterium bacterium powder, 1 part of Bacillus licheniformis powder, the red false unit cell in marsh 4 parts of bacterium bacterium powder, 4 parts of jelly bacillus bacterium powder, white 2 parts of light gray streptomycete bacterium powder, 2 parts of streptomyces tendae bacterium powder and sugarcane Uncommon 1 part of the Salmonella bacterium powder of orchid, dark red 1 part of rotation silk actinomyces bacterium powder, 1 part of streptomyces roseochromogenes bacterium powder, 1 part of cyromazine, fulvic acid 2 parts of potassium, 43 parts of diatomite.
3. a kind of preparation method of the microbe additive as claimed in claim 1 or 2 for ox treatment for cow manure, its feature It is, including following operating procedure:Each raw material of above-mentioned parts by weight is taken, uniform, the standing under the conditions of 28-32 DEG C is mixed and stirred for 24h, packing, is produced;
Wherein:The viable count index of each raw material is:Trichoderma reesei 2.0 × 109Cfu/g, aspergillus niger 1.5 × 1010Cfu/g, Sporotrichum thermophile 1.0 × 109Cfu/g, Trichoderma viride 2.0 × 109Cfu/g, koning trichoderma 2.0 × 109Cfu/g, denitrifying bacteria 1.0×1010Cfu/g, bacillus megaterium 2.0 × 1010Cfu/g, bacillus licheniformis 2.0 × 1010Cfu/g, marsh is red false single Born of the same parents bacterium 1.0 × 1010Cfu/g, jelly bacillus 1.0 × 1010Cfu/g, white light gray streptomycete bacterium powder 1.0 × 109Cfu/g, Tang Moral streptomycete bacterium powder 2.0 × 108Cfu/g, and thermoactinomyces sacchari bacterium powder 2.0 × 108Cfu/g, dark red rotation silk actinomyces bacterium powder 2.0×108Cfu/g, streptomyces roseochromogenes bacterium powder 2.0 × 108cfu/g。
4. a kind of microbe additive as claimed in claim 1 or 2 for ox treatment for cow manure raises the application on bed in milk cow, It is characterized in that:The fermented cow dung dirt for adding the microbe additive for being used for ox treatment for cow manure is used as cow dung poultry bed.
CN201710473905.4A 2017-06-21 2017-06-21 Microbial additive for treating cattle manure and preparation method and application thereof Active CN107129953B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201710473905.4A CN107129953B (en) 2017-06-21 2017-06-21 Microbial additive for treating cattle manure and preparation method and application thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201710473905.4A CN107129953B (en) 2017-06-21 2017-06-21 Microbial additive for treating cattle manure and preparation method and application thereof

Publications (2)

Publication Number Publication Date
CN107129953A true CN107129953A (en) 2017-09-05
CN107129953B CN107129953B (en) 2021-02-19

Family

ID=59736139

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201710473905.4A Active CN107129953B (en) 2017-06-21 2017-06-21 Microbial additive for treating cattle manure and preparation method and application thereof

Country Status (1)

Country Link
CN (1) CN107129953B (en)

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107509433A (en) * 2017-09-19 2017-12-26 孙立成 A kind of fertilizing method of the high sugar content spring maize of high yield
CN108117415A (en) * 2017-12-19 2018-06-05 浙江沃华环境科技有限公司 A kind of complex microorganism bacterium solution and the decomposed method of poultry urine
CN111925964A (en) * 2020-08-21 2020-11-13 扬州大学 Bacillus megaterium and application thereof
CN114315027A (en) * 2021-12-28 2022-04-12 生态环境部华南环境科学研究所 Harmless treatment method for natural storage of liquid manure and natural storage pool thereof
CN114958641A (en) * 2021-09-27 2022-08-30 上海玖霖环保科技有限公司 Environment-friendly biological agent for treating livestock and poultry manure organic garbage
CN116103204A (en) * 2023-02-28 2023-05-12 山东中新农业发展有限公司 Microbial decomposing inoculant for organic compost and preparation method thereof
CN116514605A (en) * 2023-05-05 2023-08-01 山东健源生物科技有限公司 Livestock and poultry manure microbial organic fertilizer for improving soil and preparation method thereof
CN117402020A (en) * 2023-12-13 2024-01-16 中农金瑞肥业有限公司 Yield-increasing biological organic fertilizer and preparation method thereof

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101914443A (en) * 2010-07-14 2010-12-15 周剑平 Complex microbial inoculum for swine production by fermentation bed started quickly at low temperature and preparation method thereof
CN102531766A (en) * 2011-09-15 2012-07-04 北京世纪阿姆斯生物技术股份有限公司 Microbial decomposing agent and production method thereof
CN105296394A (en) * 2015-11-04 2016-02-03 广西多得乐生物科技有限公司 Microbial decomposition agent for animal wastes and straws and preparation method thereof

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101914443A (en) * 2010-07-14 2010-12-15 周剑平 Complex microbial inoculum for swine production by fermentation bed started quickly at low temperature and preparation method thereof
CN102531766A (en) * 2011-09-15 2012-07-04 北京世纪阿姆斯生物技术股份有限公司 Microbial decomposing agent and production method thereof
CN105296394A (en) * 2015-11-04 2016-02-03 广西多得乐生物科技有限公司 Microbial decomposition agent for animal wastes and straws and preparation method thereof

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
BLOOR JMG: "Additive effects of dung amendment and plant species identity on soil processes and soil inorganic nitrogen in grass monocultures", 《PLANT AND SOIL》 *
施庆珊等: "一株高温放线菌及其在造纸污泥堆肥过程中的应用", 《农业环境科学学报》 *
汪保根等: "应用发酵牛粪垫料提高奶牛生产性能的研究", 《安徽农业科学》 *

Cited By (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107509433A (en) * 2017-09-19 2017-12-26 孙立成 A kind of fertilizing method of the high sugar content spring maize of high yield
CN108117415A (en) * 2017-12-19 2018-06-05 浙江沃华环境科技有限公司 A kind of complex microorganism bacterium solution and the decomposed method of poultry urine
CN111925964A (en) * 2020-08-21 2020-11-13 扬州大学 Bacillus megaterium and application thereof
CN111925964B (en) * 2020-08-21 2022-04-22 扬州大学 Bacillus megaterium and application thereof
CN114958641A (en) * 2021-09-27 2022-08-30 上海玖霖环保科技有限公司 Environment-friendly biological agent for treating livestock and poultry manure organic garbage
CN114315027A (en) * 2021-12-28 2022-04-12 生态环境部华南环境科学研究所 Harmless treatment method for natural storage of liquid manure and natural storage pool thereof
CN114315027B (en) * 2021-12-28 2023-01-06 生态环境部华南环境科学研究所 Harmless treatment method for natural storage of liquid manure and natural storage pool thereof
CN116103204A (en) * 2023-02-28 2023-05-12 山东中新农业发展有限公司 Microbial decomposing inoculant for organic compost and preparation method thereof
CN116103204B (en) * 2023-02-28 2024-02-23 山东中新农业发展有限公司 Microbial decomposing inoculant for organic compost and preparation method thereof
CN116514605A (en) * 2023-05-05 2023-08-01 山东健源生物科技有限公司 Livestock and poultry manure microbial organic fertilizer for improving soil and preparation method thereof
CN117402020A (en) * 2023-12-13 2024-01-16 中农金瑞肥业有限公司 Yield-increasing biological organic fertilizer and preparation method thereof
CN117402020B (en) * 2023-12-13 2024-03-15 中农金瑞肥业有限公司 Yield-increasing biological organic fertilizer and preparation method thereof

Also Published As

Publication number Publication date
CN107129953B (en) 2021-02-19

Similar Documents

Publication Publication Date Title
CN107129953A (en) A kind of microbe additive and preparation method and application for ox treatment for cow manure
CN101869184B (en) Microbial feed additive and preparation method thereof
CN101317625B (en) Comprehensive treatment method for pollution of livestock and poultry aquaculture, special feedstuff bridging agent, biological edible warm pad and biological organic fertilizer
CN101874445B (en) Method for culturing organic Chinese chives
CN105218254A (en) A kind of Microbial and ecological organic fertilizers material and preparation method thereof
CN106260541A (en) The method producing cattle and sheep complete feed for raw material ferment in second time with Ramulus Mori
CN104529573A (en) Multi-effect ferment fertilizer prepared from agricultural wastes
CN103039727B (en) Preparation method of stichopus japonicus fermented feed capable of replacing alga
CN104232551A (en) Pig-chicken excrement fermenting compound bacteria, preparation method and method of utilizing compound bacteria to ferment and prepare organic fertilizer
CN104255538A (en) Technology for preparing fermentation bed padding by recycling livestock excrements and crop straws
CN101390566A (en) Manifold microbe mixed culture fermentation agent and method for producing high energy protein biology feedstuff
CN105176881A (en) High-efficiency engineering bacteria agent and method for producing active biological organic fertilizer
CN105255767B (en) A kind of complex microorganism preparations and fowl fermenting bed padding
CN106212916B (en) The method for producing cattle and sheep complete feed as raw material staged fermentation using sugarcane tail
CN106173365A (en) The method producing cattle and sheep complete feed for raw material ferment in second time with Flos Jasmini Sambac slag
CN103274831A (en) Method for preparing traditional Chinese medicine microbial fertilizer of spinach
CN100408673C (en) Organic microbial composite and use
CN106173364A (en) The method producing cattle and sheep complete feed for raw material ferment in second time with corn straw
CN107311707A (en) A kind of preparation method of selenium-enriched earthworm organic foliar fertilizer
CN106173361A (en) The method producing cattle and sheep complete feed for raw material ferment in second time with herbage
CN104293711A (en) Sheep-and-cow dung fermented compound bacteria, preparation method thereof and method for preparing organic fertilizer by utilizing fermentation of compound bacteria
CN104232728B (en) Rapeseed peptide (rsp) albumen and preparation method thereof
CN106234755B (en) The method for producing cattle and sheep complete feed as raw material staged fermentation using bagasse
CN106212915A (en) Ferment in second time produces method and the product of cattle and sheep complete feed
CN114586890A (en) Method for preparing composite flora feed by utilizing juncao

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
TA01 Transfer of patent application right

Effective date of registration: 20180503

Address after: 271000 building 7, Wanda Plaza, Taishan District, Tai'an, Shandong, 1018

Applicant after: Shandong Jianyuan Biotechnology Co., Ltd.

Address before: 271000 building 7, Wanda Plaza, Taishan District, Tai'an, Shandong, 1018

Applicant before: Wang Cheng

TA01 Transfer of patent application right
GR01 Patent grant
GR01 Patent grant