CN107311707A - A kind of preparation method of selenium-enriched earthworm organic foliar fertilizer - Google Patents
A kind of preparation method of selenium-enriched earthworm organic foliar fertilizer Download PDFInfo
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- CN107311707A CN107311707A CN201710371259.0A CN201710371259A CN107311707A CN 107311707 A CN107311707 A CN 107311707A CN 201710371259 A CN201710371259 A CN 201710371259A CN 107311707 A CN107311707 A CN 107311707A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F1/00—Fertilisers made from animal corpses, or parts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Abstract
The present invention relates to a kind of preparation method of selenium-enriched earthworm organic foliar fertilizer, it comprises the following steps：1) stalk is handled：Stalk is crushed, is placed in together with ammoniacal liquor in obturator, microwave softened is carried out, softening stalk is obtained；2) preparation of matrix is cultivated：Animal wastes, softening stalk, microbial inoculum are mixed in proportion with sodium selenite, fermentation maturity is carried out, end of becoming thoroughly decomposed adds phagostimulant, mixes, and obtains earthworm cultivation substratum；3) feeding management：Earthworm cultivation substratum is laid on culturing bed, carries out vermiculture, obtains selenium-enriched earthworm and selenium-enriched earthworm excrement；4) earthworm is digested：Digested using the enzyme of earthworm itself, the water extract of selenium-enriched earthworm excrement is added in enzymolysis liquid is obtained, is stirred, filtered after sterilizing, obtain selenium-enriched earthworm organic foliar fertilizer.The inventive method has straw utilization rate high, and earthworm growth is quick, and selenium-rich effect is efficient, the advantage such as organic selenium concentration height of foliar fertilizer for having no toxic side effect and preparing to earthworm.
Description
Technical field
The present invention relates to a kind of preparation method of organic fertilizer, more particularly to a kind of preparation side of selenium-enriched earthworm organic foliar fertilizer
Method.
Background technology
Selenium is the necessary trace element of humans and animals, and it can promote the growth of animal, and development, raising immunity and reduction are dead
Rate is died, but China belongs to the country of serious selenium deficiency, is tiltedly worn from three provinces in the northeast of China to the Yunnan-Guizhou Plateau, accounts for Chinese area
There is one low selenium area in 72% area, wherein 30% is that serious selenium deficiency is regional, the natural food Se content such as grain is relatively low.China
The big and medium-sized cities such as north, northeast, northwest belong to selenium deficiency area.Some areas of middle 12 provinces and cities of state-two, about 700,000,000 people life
In low selenium area, most area Selenium In Foods content is in below 0.02mg/kg, and southeastern coast is Chinese district rich in selenium, also only
There is 0.10mg/kg, be minimum as defined in the United Nations's health organization.Do not reach daily that everyone is 40 micro- in Chinese population food still
Standard selenium-supply level more than gram, especially selenium deficiency area crowd, it more difficult to the purpose of selenium-supply is reached from food.So in order to
Make selenium element that plant absorption is enough and then obtain the food of selenium-rich, it is necessary to which the selenium member of external source is added to the plant in poor selenium soil
Element.
Earthworm contains the nutriments such as abundant amino acid, vitamin and the mineral matter element of a large amount of protein, species, is one
Plant good amino acid foliage fertilizer making material.The foliar fertilizer prepared with earthworm, which has, absorbs fast, strong, the low-consuming effect of effect
The advantages of rate is high.Though earthworm is to inorganic selenium with certain enrichment, inorganic selenium is to earthworm also toxic side effect, grandson
The research of small striking (2014) shows that the survival rate of earthworm is reduced with the raising of inorganic Se content in cultivation matrix, causes
Earthworm selenium-rich effect it is not good.
The content of the invention
The technical problem to be solved in the present invention is to provide a kind of preparation side of high, the simple for production foliar fertilizer of organic selenium concentration
Method.
In order to solve the above-mentioned technical problem, the present invention is achieved by the following technical solutions：A kind of selenium-enriched earthworm is organic
The preparation method of foliar fertilizer, it comprises the following steps：
1) stalk is handled：Stalk is crushed, is placed in together with ammoniacal liquor in obturator, microwave softened is carried out, softening terminates,
Obtain softening stalk；
2) preparation of matrix is cultivated：Animal wastes, softening stalk, microbial inoculum are mixed in proportion with sodium selenite, sent out
Ferment becomes thoroughly decomposed, and adds phagostimulant after end of becoming thoroughly decomposed, mixes, obtains earthworm cultivation substratum；
3) feeding management：Earthworm cultivation substratum is laid on culturing bed, is added earthworm seedling, is cultivated, and keeps earthworm
The water content of matrix is cultivated, every 5~10 days addition identical earthworm cultivation substratums and phagostimulant is sprayed on surface, cultivates 45
~50 days, obtain selenium-enriched earthworm and selenium-enriched earthworm excrement；
4) earthworm is digested：By selenium-enriched earthworm clear water washes clean, homogenate is digested using the enzyme of earthworm itself, obtained
Enzymolysis liquid is obtained, water extract, the photosynthetic bacteria of selenium-enriched earthworm excrement are added in enzymolysis liquid, is fermented under stirring, fermentation knot
Clasmatosis is carried out after beam, is filtered, selenium-enriched earthworm organic foliar fertilizer is obtained.
Preferably, described stalk is the one or more in maize straw, wheat stalk, rice straw, powder of straw
Broken size is 0.01~1cm, and the mass fraction of described ammoniacal liquor is 10~20%, and the mass ratio of stalk and ammoniacal liquor is 10：(3
~
5), the time of described microwave softened is 5~10h, and temperature is 110~150 DEG C.
Preferably, described animal wastes are the one or more in pig manure, cow dung, chicken manure, described animal wastes,
The mass ratio for softening stalk, microbial inoculum and sodium selenite mixture each component is (40~50)：(30~50)：(1~3)：(0.05~
0.1)。
Preferably, described microbial inoculum is saccharomycete, lactic acid bacteria, bacillus subtilis, photosynthetic bacteria, actinomyces, green
In trichoderma and white-rot fungi, wherein microbial inoculum mass percent number shared by each component be saccharomycete 15~25%, lactic acid bacteria 10~
15%th, bacillus subtilis 10~15%, photosynthetic bacteria 5~10%, actinomyces 10~15%, Trichoderma viride 10~15% and white
Rotten fungi 25~35%.
Preferably, the time of described fermentation maturity is 6~8 days, and the addition of described phagostimulant is cultivation matrix
The 2~5% of gross mass.
Preferably, the preparation method of described phagostimulant is that 1~2Kg rotten melon and fruit is worn into slurries, adds sub- selenium
Sour 10~20mg of sodium, 100~200g of molasses, 10~20g of microcrystalline cellulose and 10~20g of hawthorn powder are well mixed to be obtained.
Preferably, described rotten melon and fruit is wax gourd, balsam pear, apple, banana, pears, pineapple, watermelon, muskmelon, Hami
One or more in melon, mango.
Preferably, described earthworm seedling is compatriot love victory earthworm, and the water content of described earthworm cultivation substratum is 50
~70%.
Preferably, the time of enzymolysis is 10~15h, enzymolysis liquid, the water extract of selenium-enriched earthworm excrement and photosynthetic bacteria
Mass ratio is 10:(10~30)：(0.5~2), fermentation time is 5~10 days, and the method for described clasmatosis is to use
Ultrasonic wave carries out clasmatosis, and the method for described filtering is to be filtered using 0.45 μm of filter membrane.
Advantages of the present invention：The stalk softening method of the inventive method, bating effect good feature short with the softening time,
The utilization rate more than 10% of stalk can be improved；Stalk with animal wastes add sodium selenite together with fermentation maturity, selenous acid
Sodium is converted to Organic Selenium through microbial action, reduces toxicity of the selenium element to earthworm, improves the ability of earthworm selenium-rich, make
The selenium rich ability of earthworm improves more than 300%, and stalk passes through the decomposition of microorganism, adds mycoprotein, also makes stalk
Utilization rate improve more than 5%；Phagostimulant is added in cultivation matrix, the desire of ingesting of earthworm can be increased, strengthen its digestible energy
Power, promotes it to grow, and makes the output increased more than 10% of earthworm；Sodium selenite causes system by the multiple trans-utilization of organism
Standby foliar fertilizer has organic selenium content high, it is easy to the features such as plant absorption is utilized, and improves the content of Organic Selenium in plant
More than 10%；The water extract of enzymolysis liquid and selenium-enriched earthworm excrement passes through the fermentation again of photosynthetic bacteria, makes obtained foliar fertilizer tool
There are the physiologically actives such as species abundant amino acid, mineral matter, various B family vitamins, somatomedin, ubiquinone and disease-resistant factor
Material, the cytoactive of energy activated plant, improves photosynthesis more than 15%, strengthens the resistance against diseases of plant；The inventive method
The foliar fertilizer of preparation have make simple, steady quality, fertilizer efficiency it is higher, using scope it is wide the features such as.
Embodiment
Further advantage, target and the feature of the present invention embodies part by following explanation, and part will also be by this
The research and practice of invention and be understood by the person skilled in the art.
Following embodiments are used to further illustrate but be not limited to the present invention.
A kind of a kind of way of realization of the preparation method of selenium-enriched earthworm organic foliar fertilizer of the present invention of embodiment 1, it includes
Following steps：
1) stalk is handled：Stalk is crushed, is placed in together with ammoniacal liquor in obturator, microwave softened is carried out, softening terminates,
Obtain softening stalk；
2) preparation of matrix is cultivated：Animal wastes, softening stalk, microbial inoculum are mixed in proportion with sodium selenite, sent out
Ferment becomes thoroughly decomposed, and end of becoming thoroughly decomposed adds phagostimulant, mixes, and obtains earthworm cultivation substratum；
3) feeding management：Earthworm cultivation substratum is laid on culturing bed, is added earthworm seedling, is cultivated, and keeps earthworm
The water content of matrix is cultivated, every 5 days addition identical earthworm cultivation substratums and phagostimulant is sprayed on surface, cultivates 45 days, obtain
Obtain selenium-enriched earthworm and selenium-enriched earthworm excrement；
4) earthworm is digested：By selenium-enriched earthworm clear water washes clean, homogenate is digested using the enzyme of earthworm itself, obtained
Enzymolysis liquid is obtained, water extract, the photosynthetic bacteria of selenium-enriched earthworm excrement are added in enzymolysis liquid, is fermented under stirring, fermentation knot
Clasmatosis is carried out after beam, is filtered, selenium-enriched earthworm organic foliar fertilizer is obtained.
It is longer the time required to straw ammoniation softening, the effect of microwave is added during ammonification softens, can effectively be subtracted
The time softened less, the efficiency of softening is improved, while the bating effect for the stalk that the effect of microwave also makes is more preferable；Stalk is through soft
Fermented after change together with animal wastes, the degradation rate of stalk can be improved；Sodium selenite is added during becoming thoroughly decomposed, nothing can be made
The sodium selenite of machine is converted into Organic Selenium, and reduction inorganic selenium also causes the effect of earthworm bioaccumulation selenium more to the toxic side effect of earthworm
Ideal, compared with the concentration effect of inorganic selenium is used alone, makes the selenium-rich effect of earthworm high more than 3 times using Organic Selenium；Sub- selenium
Sour sodium passes through bacterium, and earthworm, the multiple trans-utilization of bacterium causes the foliar fertilizer prepared to have organic selenium content high, it is easy to
The features such as plant absorption is utilized, compared with the effect of earthworm bioaccumulation selenium is used alone, having for acquisition is repeatedly converted by organism
Machine selenium can make the content of Organic Selenium in plant improve more than 10% when in use；The water extract of enzymolysis liquid and selenium-enriched earthworm excrement
By the fermentation again of photosynthetic bacteria, make obtained foliar fertilizer that there is the abundant amino acid of species, mineral matter, the dimension life of various B races
The physiological activators such as element, somatomedin, ubiquinone and disease-resistant factor, the cytoactive of energy activated plant improves photosynthetic make
With more than 15%, strengthen the resistance against diseases of plant.
A kind of a kind of way of realization of the preparation method of selenium-enriched earthworm organic foliar fertilizer of the present invention of embodiment 2, it includes
Following steps：
1) stalk is handled：By 100Kg corn straw smashing, 1cm sizes are ground into, are 10% with 50Kg mass fractions
Ammoniacal liquor is placed in obturator together, carries out microwave heating and softening, and temperature is 110 DEG C, and time 10h, softening terminates, and is obtained soft
Change stalk；
2) preparation of matrix is cultivated：Animal wastes, softening stalk, microbial inoculum are mixed in proportion with sodium selenite, sent out
Ferment becomes thoroughly decomposed, and end of becoming thoroughly decomposed adds phagostimulant, mixes, and obtains earthworm cultivation substratum；
3) feeding management：Earthworm cultivation substratum is laid on culturing bed, is added earthworm seedling, is cultivated, and keeps earthworm
The water content of matrix is cultivated, every 10 days addition identical earthworm cultivation substratums and phagostimulant is sprayed on surface, cultivates 50 days,
Obtain selenium-enriched earthworm and selenium-enriched earthworm excrement；
4) earthworm is digested：By selenium-enriched earthworm clear water washes clean, homogenate is digested using the enzyme of earthworm itself, obtained
Enzymolysis liquid is obtained, water extract, the photosynthetic bacteria of selenium-enriched earthworm excrement are added in enzymolysis liquid, is fermented under stirring, fermentation knot
Clasmatosis is carried out after beam, is filtered, selenium-enriched earthworm organic foliar fertilizer is obtained.
Composition (such as cellulose, hemicellulose are decomposed) decomposed is easily decomposed in the heating process of microwave, in stalk, is made
Ammonia is more easy to occur ammonolysis reaction with organic substance, by ammonolysis reaction, and ester bond between destruction lignin and polysaccharide weakens wooden
Contacting between element and cellulose, is made stalk be easier to be decomposed by the microorganisms, compared with the effect only softened using ammonia, carried
High straw utilization rate more than 10%.
A kind of a kind of way of realization of the preparation method of selenium-enriched earthworm organic foliar fertilizer of the present invention of embodiment 3, it includes
Following steps：
1) stalk is handled：By 100Kg corn straw smashing, 0.5cm sizes are ground into, are 20% with 30Kg mass fractions
Ammoniacal liquor be placed in together in obturator, carry out microwave heating and softening, temperature be 150 DEG C, time 5h, softening terminates, obtain
Soften stalk；
2) preparation of matrix is cultivated：Swine excrement, softening stalk, microbial inoculum are mixed in proportion with sodium selenite, described ox
Excrement, softening stalk, the mass ratio of microbial inoculum and sodium selenite mixture each component are 50：30：3：0.05, fermentation maturity is carried out,
Become thoroughly decomposed and add phagostimulant after terminating, mix, obtain earthworm cultivation substratum；
3) feeding management：Earthworm cultivation substratum is laid on culturing bed, is added earthworm seedling, is cultivated, and keeps earthworm
The water content of matrix is cultivated, identical earthworm cultivation substratum was added every 10 days, is cultivated 50 days, selenium-enriched earthworm and selenium-rich is obtained
Wormcast；
4) earthworm is digested：By selenium-enriched earthworm clear water washes clean, homogenate is digested using the enzyme of earthworm itself, obtained
Enzymolysis liquid is obtained, water extract, the photosynthetic bacteria of selenium-enriched earthworm excrement are added in enzymolysis liquid, is fermented under stirring, fermentation knot
Clasmatosis is carried out after beam, is filtered, selenium-enriched earthworm organic foliar fertilizer is obtained.
Effective decomposition can be carried out to stalk by adding microbial inoculum, and it is mycoprotein to make the carbohydrate-modifying of stalk,
Make earthworm digesting and assimilating more fully to stalk, the utilization rate more than 5% of raising stalk.
A kind of a kind of way of realization of the preparation method of selenium-enriched earthworm organic foliar fertilizer of the present invention of embodiment 4, it includes
Following steps：
1) stalk is handled：By 100Kg corn straw smashing, 0.5cm sizes are ground into, are 15% with 40Kg mass fractions
Ammoniacal liquor be placed in together in obturator, carry out microwave heating and softening, temperature be 130 DEG C, time 7h, softening terminates, obtain
Soften stalk；
2) preparation of matrix is cultivated：By cow dung just, softening stalk, microbial inoculum mixed in proportion with sodium selenite, described ox
Excrement, softening stalk, the mass ratio of microbial inoculum and sodium selenite mixture each component are 50：30：3：0.05, described microbial inoculum is
It is each in saccharomycete, lactic acid bacteria, bacillus subtilis, photosynthetic bacteria, actinomyces, Trichoderma viride and white-rot fungi, wherein microbial inoculum
Mass percent number shared by component is saccharomycete 15%, lactic acid bacteria 10%, bacillus subtilis 10%, photosynthetic bacteria 10%, put
Line bacterium 10%, Trichoderma viride 15% and white-rot fungi 30%, carry out fermentation maturity, and end of becoming thoroughly decomposed adds phagostimulant, mixes, obtains
Obtain earthworm cultivation substratum；
3) feeding management：Earthworm cultivation substratum is laid on culturing bed, is added earthworm seedling, is cultivated, and keeps earthworm
The water content of matrix is cultivated, every 7 days addition identical earthworm cultivation substratums and phagostimulant is sprayed on surface, cultivates 47 days, obtain
Obtain selenium-enriched earthworm and selenium-enriched earthworm excrement；
4) earthworm is digested：By selenium-enriched earthworm clear water washes clean, homogenate is digested using the enzyme of earthworm itself, obtained
Enzymolysis liquid is obtained, water extract, the photosynthetic bacteria of selenium-enriched earthworm excrement are added in enzymolysis liquid, is fermented under stirring, fermentation knot
Clasmatosis is carried out after beam, is filtered, selenium-enriched earthworm organic foliar fertilizer is obtained.
A kind of a kind of way of realization of the preparation method of selenium-enriched earthworm organic foliar fertilizer of the present invention of embodiment 5, it includes
Following steps：
1) stalk is handled：By 100Kg corn straw smashing, 0.5cm sizes are ground into, are 15% with 40Kg mass fractions
Ammoniacal liquor be placed in together in obturator, carry out microwave heating and softening, temperature be 130 DEG C, time 7h, softening terminates, obtain
Soften stalk；
2) preparation of matrix is cultivated：Chicken manure, softening stalk, microbial inoculum are mixed in proportion with sodium selenite, described chicken
Excrement, softening stalk, the mass ratio of microbial inoculum and sodium selenite mixture each component are 40：50：1：0.1, described microbial inoculum is ferment
Each component in female bacterium, lactic acid bacteria, bacillus subtilis, photosynthetic bacteria, actinomyces, Trichoderma viride and white-rot fungi, wherein microbial inoculum
Shared mass percent number is saccharomycete 15%, lactic acid bacteria 10%, bacillus subtilis 10%, photosynthetic bacteria 10%, actinomyces
10%th, Trichoderma viride 15 and white-rot fungi 30%, carry out fermentation maturity 6 days, and end of becoming thoroughly decomposed adds earthworm cultivation substratum gross mass
5% phagostimulant, is mixed, and obtains earthworm cultivation substratum；
3) feeding management：Earthworm cultivation substratum is laid on culturing bed, is added earthworm seedling, is cultivated, and keeps earthworm
The water content of matrix is cultivated, every 8 days addition identical earthworm cultivation substratums and phagostimulant is sprayed on surface, cultivates 46 days, obtain
Obtain selenium-enriched earthworm and selenium-enriched earthworm excrement；
4) earthworm is digested：By selenium-enriched earthworm clear water washes clean, homogenate is digested using the enzyme of earthworm itself, obtained
Enzymolysis liquid is obtained, water extract, the photosynthetic bacteria of selenium-enriched earthworm excrement are added in enzymolysis liquid, is fermented under stirring, fermentation knot
Clasmatosis is carried out after beam, is filtered, selenium-enriched earthworm organic foliar fertilizer is obtained.
Stalk is even across sofening treatment, but its content of cellulose is high, and nutriment is few, and earthworm is not liked to ingest, and increase is lured
Food agent can increase the desire of ingesting of earthworm, can not only increase the ratio of stalk in cultivation matrix, moreover it is possible to promote the growth of earthworm,
Make the output increased more than 10% of earthworm.
A kind of a kind of way of realization of the preparation method of selenium-enriched earthworm organic foliar fertilizer of the present invention of embodiment 6, it includes
Following steps：
1) stalk is handled：By 100Kg corn straw smashing, 0.5cm sizes are ground into, are placed in obturator, added
40Kg mass fractions are 15% ammoniacal liquor, carry out microwave heating and softening, and temperature is 130 DEG C, and time 7h, softening terminates, and is obtained soft
Change stalk；
2) preparation of matrix is cultivated：Chicken manure, softening stalk, microbial inoculum are mixed in proportion with sodium selenite, described chicken
Excrement, softening stalk, the mass ratio of microbial inoculum and sodium selenite mixture each component are 40：50：1：0.1, described microbial inoculum is ferment
Each component in female bacterium, lactic acid bacteria, bacillus subtilis, photosynthetic bacteria, actinomyces, Trichoderma viride and white-rot fungi, wherein microbial inoculum
Shared mass percent number is saccharomycete 15%, lactic acid bacteria 10%, bacillus subtilis 10%, photosynthetic bacteria 10%, actinomyces
10%th, Trichoderma viride 15% and white-rot fungi 30%, carry out fermentation maturity 6 days, and end of becoming thoroughly decomposed adds the total matter of earthworm cultivation substratum
The phagostimulant of amount 5%, the preparation method of described phagostimulant is that 1Kg rotten melon and fruit is worn into slurries, adds sodium selenite
10mg, molasses 100g, microcrystalline cellulose 10g and hawthorn powder 10g, earthworm cultivation substratum are mixed with phagostimulant, obtain vermiculture
Matrix；
3) feeding management：Earthworm cultivation substratum is laid on culturing bed, is added earthworm seedling, is cultivated, and keeps earthworm
The water content of matrix is cultivated, every 8 days addition identical earthworm cultivation substratums and phagostimulant is sprayed on surface, cultivates 46 days, obtain
Obtain selenium-enriched earthworm and selenium-enriched earthworm excrement；
4) earthworm is digested：By selenium-enriched earthworm clear water washes clean, homogenate is digested using the enzyme of earthworm itself, obtained
Enzymolysis liquid is obtained, water extract, the photosynthetic bacteria of selenium-enriched earthworm excrement are added in enzymolysis liquid, is fermented under stirring, fermentation knot
Clasmatosis is carried out after beam, is filtered, selenium-enriched earthworm organic foliar fertilizer is obtained.
It can increase the desire of ingesting of earthworm by adding molasses, promote the growth of earthworm；It can be increased by adding microcrystalline cellulose
Plus in earthworm body cellulase secretion, improve stalk digestibility more than 5%；It can be stimulated by adding hawthorn powder in earthworm
Chamber, makes digestion rate accelerate more than 5%.
A kind of a kind of way of realization of the preparation method of selenium-enriched earthworm organic foliar fertilizer of the present invention of embodiment 7, it includes
Following steps：
1) stalk is handled：By 100Kg corn straw smashing, 0.5cm sizes are ground into, are 15% with 40Kg mass fractions
Ammoniacal liquor be placed in together in obturator, carry out microwave heating and softening, temperature be 130 DEG C, time 7h, softening terminates, obtain
Soften stalk；
2) preparation of matrix is cultivated：Chicken manure, softening stalk, microbial inoculum are mixed in proportion with sodium selenite, described chicken
Excrement, softening stalk, the mass ratio of microbial inoculum and sodium selenite mixture each component are 40：50：1：0.1, described microbial inoculum is ferment
Each component in female bacterium, lactic acid bacteria, bacillus subtilis, photosynthetic bacteria, actinomyces, Trichoderma viride and white-rot fungi, wherein microbial inoculum
Shared mass percent number is saccharomycete 15%, lactic acid bacteria 10%, bacillus subtilis 10%, photosynthetic bacteria 10%, actinomyces
10%th, Trichoderma viride 15 and white-rot fungi 30%, carry out fermentation maturity 6 days, and end of becoming thoroughly decomposed adds earthworm cultivation substratum gross mass
5% phagostimulant, the preparation method of described phagostimulant is that 2Kg rotten banana is worn into slurries, adds sodium selenite
20mg, molasses 200g, microcrystalline cellulose 20g and hawthorn powder 20g, earthworm cultivation substratum are mixed with phagostimulant, obtain vermiculture
Matrix；
3) feeding management：Earthworm cultivation substratum is laid on culturing bed, is added earthworm seedling, is cultivated, and keeps earthworm
The water content of matrix is cultivated, every 8 days addition identical earthworm cultivation substratums and phagostimulant is sprayed on surface, cultivates 46 days, obtain
Obtain selenium-enriched earthworm and selenium-enriched earthworm excrement；
4) earthworm is digested：By selenium-enriched earthworm clear water washes clean, homogenate is digested using the enzyme of earthworm itself, obtained
Enzymolysis liquid is obtained, water extract, the photosynthetic bacteria of selenium-enriched earthworm excrement are added in enzymolysis liquid, is fermented under stirring, fermentation knot
Clasmatosis is carried out after beam, is filtered, selenium-enriched earthworm organic foliar fertilizer is obtained.
A kind of a kind of way of realization of the preparation method of selenium-enriched earthworm organic foliar fertilizer of the present invention of embodiment 8, it includes
Following steps：
1) stalk is handled：By 100Kg corn straw smashing, 0.5cm sizes are ground into, are 15% with 40Kg mass fractions
Ammoniacal liquor be placed in together in obturator, carry out microwave heating and softening, temperature be 130 DEG C, time 7h, softening terminates, obtain
Soften stalk；
2) preparation of matrix is cultivated：Chicken manure, softening stalk, microbial inoculum are mixed in proportion with sodium selenite, described chicken
Excrement, softening stalk, the mass ratio of microbial inoculum and sodium selenite mixture each component are 40：50：1：0.1, described microbial inoculum is ferment
Each component in female bacterium, lactic acid bacteria, bacillus subtilis, photosynthetic bacteria, actinomyces, Trichoderma viride and white-rot fungi, wherein microbial inoculum
Shared mass percent number is saccharomycete 15%, lactic acid bacteria 10%, bacillus subtilis 10%, photosynthetic bacteria 10%, actinomyces
10%th, Trichoderma viride 15% and white-rot fungi 30%, carry out fermentation maturity 6 days, and end of becoming thoroughly decomposed adds the total matter of earthworm cultivation substratum
The phagostimulant of amount 5%, the preparation method of described phagostimulant is that 2Kg rotten pawpaw is worn into slurries, adds sodium selenite
20mg, molasses 200g, microcrystalline cellulose 20g and hawthorn powder 20g, earthworm cultivation substratum are mixed with phagostimulant, obtain vermiculture
Matrix；
3) feeding management：Earthworm cultivation substratum is laid on culturing bed, is added compatriot's love victory earthworm seedling, is cultivated,
The water content 50% of earthworm cultivation substratum is kept, every 8 days addition identical earthworm cultivation substratums and food calling is sprayed on surface
Agent, is cultivated 46 days, obtains selenium-enriched earthworm and selenium-enriched earthworm excrement；
4) earthworm is digested：By selenium-enriched earthworm clear water washes clean, homogenate is digested using the enzyme of earthworm itself, obtained
Enzymolysis liquid is obtained, water extract, the photosynthetic bacteria of selenium-enriched earthworm excrement are added in enzymolysis liquid, is fermented under stirring, fermentation knot
Clasmatosis is carried out after beam, is filtered, selenium-enriched earthworm organic foliar fertilizer is obtained.
Compatriot love victory earthworm has the advantages that fast, individual small, the meat ratio of strong adaptability, breeding is high.
A kind of a kind of way of realization of the preparation method of selenium-enriched earthworm organic foliar fertilizer of the present invention of embodiment 9, it includes
Following steps：
1) stalk is handled：By 100Kg corn straw smashing, 0.5cm sizes are ground into, are 15% with 40Kg mass fractions
Ammoniacal liquor be placed in together in obturator, carry out microwave heating and softening, temperature be 130 DEG C, time 7h, softening terminates, obtain
Soften stalk；
2) preparation of matrix is cultivated：Chicken manure, softening stalk, microbial inoculum are mixed in proportion with sodium selenite, described chicken
Excrement, softening stalk, the mass ratio of microbial inoculum and sodium selenite mixture each component are 40：50：1：0.1, described microbial inoculum is ferment
Each component in female bacterium, lactic acid bacteria, bacillus subtilis, photosynthetic bacteria, actinomyces, Trichoderma viride and white-rot fungi, wherein microbial inoculum
Shared mass percent number is saccharomycete 15%, lactic acid bacteria 10%, bacillus subtilis 10%, photosynthetic bacteria 10%, actinomyces
10%th, Trichoderma viride 15% and white-rot fungi 30%, carry out fermentation maturity 6 days, and end of becoming thoroughly decomposed adds the total matter of earthworm cultivation substratum
The phagostimulant of amount 5%, the preparation method of described phagostimulant is that 2Kg rotten pawpaw is worn into slurries, adds sodium selenite
20mg, molasses 200g, microcrystalline cellulose 20g and hawthorn powder 20g, earthworm cultivation substratum are mixed with phagostimulant, obtain vermiculture
Matrix；
3) feeding management：Earthworm cultivation substratum is laid on culturing bed, is added compatriot's love victory earthworm seedling, is cultivated,
The water content 50% of earthworm cultivation substratum is kept, every 8 days addition identical earthworm cultivation substratums and food calling is sprayed on surface
Agent, is cultivated 46 days, obtains selenium-enriched earthworm and selenium-enriched earthworm excrement；
4) earthworm is digested：By selenium-enriched earthworm clear water washes clean, homogenate carries out enzymolysis 10h using the enzyme of earthworm itself,
Enzymolysis liquid is obtained, the mass ratio of enzymolysis liquid, the water extract of selenium-enriched earthworm excrement and photosynthetic bacteria is 10:10：0.5, during fermentation
Between be 5 days, the method for described clasmatosis is to carry out clasmatosis using ultrasonic wave, and the method for described filtering is uses
0.45 μm of filter membrane is filtered, and obtains selenium-enriched earthworm organic foliar fertilizer.
Carrying out crushing energy using ultrasonic wave makes active material in photosynthetic bacteria cell be discharged into solution, beneficial to the suction of plant
Receive and utilize；Filtration sterilization is carried out using 0.45 μm of filter membrane, obtained selenium-enriched earthworm organic foliar fertilizer is kept steady quality.
Although embodiment of the present invention is disclosed as above, it is not restricted in specification and embodiment listed
With it can be applied to various suitable the field of the invention completely, for those skilled in the art, can be easy
Other modification is realized on ground, therefore under the universal limited without departing substantially from claim and equivalency range, the present invention is not
It is limited to specific details.
Claims (9)
1. a kind of preparation method of selenium-enriched earthworm organic foliar fertilizer, it is characterised in that comprise the following steps：
1) stalk is handled：Stalk is crushed, is placed in together with ammoniacal liquor in obturator, microwave softened is carried out, softening terminates, is obtained
Soften stalk；
2) preparation of matrix is cultivated：Animal wastes, softening stalk, microbial inoculum are mixed in proportion with sodium selenite, fermentation is carried out rotten
It is ripe, phagostimulant is added after end of becoming thoroughly decomposed, is mixed, earthworm cultivation substratum is obtained；
3) feeding management：Earthworm cultivation substratum is laid on culturing bed, is added earthworm seedling, is cultivated, and keeps vermiculture
The water content of matrix, every 5~10 days addition identical earthworm cultivation substratums and sprays phagostimulant on surface, cultivates 45~50
My god, obtain selenium-enriched earthworm and selenium-enriched earthworm excrement；
4) earthworm is digested：By selenium-enriched earthworm clear water washes clean, homogenate is digested using the enzyme of earthworm itself, obtains enzyme
Liquid is solved, water extract, the photosynthetic bacteria of selenium-enriched earthworm excrement are added in enzymolysis liquid, is fermented under stirring, after fermentation ends
Clasmatosis is carried out, filtering obtains selenium-enriched earthworm organic foliar fertilizer.
2. the preparation method of selenium-enriched earthworm organic foliar fertilizer according to claim 1, it is characterised in that described stalk is
One or more in maize straw, wheat stalk, rice straw, the size that stalk is crushed is 0.01~1cm, described ammoniacal liquor
Mass fraction be 10~20%, the mass ratio of stalk and ammoniacal liquor is 10：(3~5), time of described microwave softened for 5~
10h, temperature is 110~150 DEG C.
3. the preparation method of selenium-enriched earthworm organic foliar fertilizer according to claim 1, it is characterised in that described animal excreta
Just it is the one or more in pig manure, cow dung, chicken manure, described animal wastes, softening stalk, microbial inoculum and sodium selenite mixture
The mass ratio of each component is (40~50)：(30~50)：(1~3)：(0.05~0.1).
4. the preparation method of selenium-enriched earthworm organic foliar fertilizer according to claim 1, it is characterised in that described microbial inoculum is
Each group in saccharomycete, lactic acid bacteria, bacillus subtilis, photosynthetic bacteria, actinomyces, Trichoderma viride and white-rot fungi, wherein microbial inoculum
Point shared mass percent number is saccharomycete 15~25%, lactic acid bacteria 10~15%, bacillus subtilis 10~15%, photosynthetic thin
Bacterium 5~10%, actinomyces 10~15%, Trichoderma viride 10~15% and white-rot fungi 25~35%.
5. the preparation method of selenium-enriched earthworm organic foliar fertilizer according to claim 1, it is characterised in that described fermentation is rotten
The ripe time is 6~8 days, and the addition of described phagostimulant is the 2~5% of cultivation matrix gross mass.
6. the preparation method of the selenium-enriched earthworm organic foliar fertilizer according to any one of Claims 1 to 5, it is characterised in that institute
The preparation method for the phagostimulant stated is that 1~2Kg rotten melon and fruit is worn into slurries, adds 10~20mg of sodium selenite, molasses 100
~200g, 10~20g of microcrystalline cellulose and the well mixed acquisitions of 10~20g of hawthorn powder.
7. the preparation method of selenium-enriched earthworm organic foliar fertilizer according to claim 7, it is characterised in that described rotten melon
Fruit is the one or more in wax gourd, balsam pear, apple, banana, pears, pineapple, watermelon, muskmelon, "Hami" melon, mango.
8. the preparation method of selenium-enriched earthworm organic foliar fertilizer according to claim 1, it is characterised in that described earthworm kind
Seedling is compatriot love victory earthworm, and the water content of described earthworm cultivation substratum is 50~70%.
9. the preparation method of selenium-enriched earthworm organic foliar fertilizer according to claim 1, it is characterised in that the time of enzymolysis is
10~15h, the mass ratio of enzymolysis liquid, the water extract of selenium-enriched earthworm excrement and photosynthetic bacteria is 10:(10~30)：(0.5~2),
Fermentation time is 5~10 days, and the method for described clasmatosis is to carry out clasmatosis using ultrasonic wave, the side of described filtering
Method is to be filtered using 0.45 μm of filter membrane.
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| CN113261567A (en) * | 2021-05-31 | 2021-08-17 | 天津天丰泽田生物科技有限公司 | Preparation method and application of earthworm polypeptide for promoting crop growth and resisting diseases |
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Application publication date: 20171103 |